Cucsa.143010 (gene) Cucumber (Gy14) v1

NameCucsa.143010
Typegene
OrganismCucumis sativus (Cucumber (Gy14) v1)
DescriptionNon-structural maintenance of chromosome element 4
Locationscaffold01079 : 1308929 .. 1311717 (-)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ACAACGCCAAATTTCAGTACTCAGACAAACCCCCGCTCTTTTCCTCTTCTCTTTTCTTCCTCTCCAAATTCAAAAAAAAATCAACTAAATTTCTCGATTTCATTTCTCAAATCCCCTCTCCCATTCTCTGCTTCCTCACAATTTCTCTATTTTTTCCCTGAATTCGAAACCCTATTTCAGAATTAGGGTTTCCGTTGATTAATCGTAACCGATGAGGCCTGTCAAACGCGAACCCACCGTTAGCACCCGCAGTAATCGAAATAGAGGCGGCGACGGCGGTGGCGGTGGCGGTGGCGGCGCTGTTGACGGTGCGAGTTTAAGGGATAGTAAGCGGGAGAGGGTTGCCCGGGAGAGAGAGAACTCCGGAGAGGAGAATATGAATGAAGAAGTGGGTCAGCAGGATTTGACGAAGAGGCGGGTCCTTCGCTCTCGGTACCTCGCGGTTATTCATGAGATTAGTGGTATTGTGAATCTTTGCATTTTGGATTTTGAGATGTGGGTTTGATTTGTTTGTGTGTGTAATTGGTTTTTGTTTGTTTCTTGAACTTGAAGAAAGAAGAGAGGATTTGTCGAAGGATTTGGATAAGTTTAATGTTATCATCAATGAAGTGGAAAAGTTGCACGAGCAAGGTATTTCTGTAGTCTTTTCTTATTGTTGTCCTTGTATCTAATTCTAACACAAATTTAAAGCGACGAAAGTGTGCATTTTTGCCCTTTGCCTTTGTGTTCTTATATTCATACAAGTTTTTATGTCAGTTTGAATAGTTGTTAATTTTCCTTTTGATGTTGTTATCTACGTGACATGGTCGTGTTTTCGTTTGGGTTTCAGTTCAGAAGCCAAGGGAACAAGTTGCAGATGCAGAAGCTTTGCAAGATATTGCCAACTCCCTTGTGACCTCTATTAGGTCCCAGTCAAATGAAGGTGTTACTCCATCAGATTTTGTTAGTTGTCTACTCAGAGAGTTTGCTGACATTAACGGTAGTATAAACTCTGAAGAACAAGACCTGGTTTCTGTCAATTGGAAAGATATTGGACTCAGTGTTTCGTCTATTTTCATGAATGGTTATGGATGCCGCACCATGTGAGATTTATGGATTCCTTTTTTCCACTATCCGGATCCTTAAGTGTCTTGTATTGATGTTCTTACTCAACTATATAAATTTCTTCTTTTTGTGCGTGCTTTTTTTCTTCTCAAGGCTTGGACCTATGAGCAATCAGTTGAAGCAGCGAAAGACTAATAATGTTCCCAGGAAACGTGTCAGGCCTACTGAAAGTTCCAGACCTGCAGAGGTTAGAGCAAAAAAAAATTATATTTGAAAGTACTTGGGATTTAGGATTGAGTCATCTGTCACTCTGTTTTGAGCTTTCGTTATTCTGTTGCCTTGACCTGAAAAAGGCTTTCCTTTCAAAATTCCTAGCACGTTTACTATTTCAGCACTCTATTAGTTTCGAGTCTAACAGAAGTACGGTTATTCTTTCTTGGCTGGACATACATCATTGAACTTGCTAATGTTTCATCTTGACCTTGACAAAGTTTGTTTGGAGTCACTCCTCAATGTCTGCGTTAATGACGTTCTTCACTTAACATTATCAACCTTTTTTTTTTTTGGTTTAAGTGGTCTGTGAAGTAGATTGTGAAATAGAAGTTTGTTTCTTAAGAATTTGACATTCTATCATCCCGTTTTTGATTGTTTCAATTCATACTGCAGGTTGAAGATAATGGATCCGAACCAAAAACAGATACAGATAAGAACATGGGCATCATGTTTGGAATTTTGAGGAGGAATAAAACCGTTAAACTAGAACATTTGATATTGAATAGAAAGTCTTTCGGACAGACAGTGGAGAATTTATTTGCTCTATCCTTTCTGGTTAAGGATGGTCGAGCTGAGATAACTATTGATAAAAATGGTTCTCATTTTGTGTGTGAGTAGACATCTACAAACTAAACATTTTATGCTTTTGTTTGAAAACATAGTGTTCTTTTCACTATACTAACATTTCCTGCCAACATTTTCAGCACCGAAGAATGCCCCTGCTCATAGTGCCATTATGTCACATGAAGTTAATTACAGTCATTTTGTATTCAGATTCGATTACAAGGATTGGAAGGTACGAACTTAGCATTCATGTTTATGATAATAACTTCACATATTGCGGTGTCATCTAACATACATCAAATATCAAGAAAATCATTCTGCTCCCTTGATTAGAGTGTATACAATATTGGATAGTGTTTCATGATATATGCAAAATGCTGTGTGCTATTTAGATCCTTGGATCCAATTGAGGAACACATACTTGTAATGCTTTATTTGCAGCCAATGGTGGATATGGTACCAGTTGGAGAGGAGCTGATGCCCCACAGGACCTTTCTGAACTCAGTGCAGGTGTCCCAAGAAGAAGTGGCGGCGGACAATTCTGAATCTGCAGGCTTACCTACCACACCAATCCGAAAGCTGTCGAGAAATCGAGGTTTGGTTATGCAATCTGTTGTTGAAGATTCTCCTGACAATGGCTCTCAATCAGGAGCAACTGCAATTTTGAGGGGCAAGAGAAAGCTCAGTCAAGGTTGGGTTAATTAGAGCTCTGAAAGCTACCAAATGTGAATGCCTCTTTTTGTTCTGTGAGTTGTATATTTAACGGTTGCCTTGTAAATTGGTAATTAGGTTCTAAGAGTAAATGATCGCCAAATTTAACTTTTATAACAGAGTTTCAATTAAGTTGCAATGATGATTGCAAAGTTCCATACCTTTTTGGGTAGCTTAGATCAAGCTCCAATCATTTT

mRNA sequence

ACAACGCCAAATTTCAGTACTCAGACAAACCCCCGCTCTTTTCCTCTTCTCTTTTCTTCCTCTCCAAATTCAAAAAAAAATCAACTAAATTTCTCGATTTCATTTCTCAAATCCCCTCTCCCATTCTCTGCTTCCTCACAATTTCTCTATTTTTTCCCTGAATTCGAAACCCTATTTCAGAATTAGGGTTTCCGTTGATTAATCGTAACCGATGAGGCCTGTCAAACGCGAACCCACCGTTAGCACCCGCAGTAATCGAAATAGAggcggcgacggcggtggcggtggcggtggcggcgctgttgacggtgCGAGTTTAAGGGATAGTAAGCGGGAGAGGGTTGCCCGGGAGAGAGAGAACTCCGGAGAGGAGAATATGAATGAAGAAGTGGGTCAGCAGGATTTGACGAAGAGGCGGGTCCTTCGCTCTCGGTACCTCGCGGTTATTCATGAGATTAGTGAAAGAAGAGAGGATTTGTCGAAGGATTTGGATAAGTTTAATGTTATCATCAATGAAGTGGAAAAGTTGCACGAGCAAGTTCAGAAGCCAAGGGAACAAGTTGCAGATGCAGAAGCTTTGCAAGATATTGCCAACTCCCTTGTGACCTCTATTAGGTCCCAGTCAAATGAAGGTGTTACTCCATCAGATTTTGTTAGTTGTCTACTCAGAGAGTTTGCTGACATTAACGGTAGTATAAACTCTGAAGAACAAGACCTGGTTTCTGTCAATTGGAAAGATATTGGACTCAGTGTTTCGTCTATTTTCATGAATGGTTATGGATGCCGCACCATGCTTGGACCTATGAGCAATCAGTTGAAGCAGCGAAAGACTAATAATGTTCCCAGGAAACGTGTCAGGCCTACTGAAAGTTCCAGACCTGCAGAGGTTGAAGATAATGGATCCGAACCAAAAACAGATACAGATAAGAACATGGGCATCATGTTTGGAATTTTGAGGAGGAATAAAACCGTTAAACTAGAACATTTGATATTGAATAGAAAGTCTTTCGGACAGACAGTGGAGAATTTATTTGCTCTATCCTTTCTGGTTAAGGATGGTCGAGCTGAGATAACTATTGATAAAAATGGTTCTCATTTTGTGTCACCGAAGAATGCCCCTGCTCATAGTGCCATTATGTCACATGAAGTTAATTACAGTCATTTTGTATTCAGATTCGATTACAAGGATTGGAAGCCAATGGTGGATATGGTACCAGTTGGAGAGGAGCTGATGCCCCACAGGACCTTTCTGAACTCAGTGCAGGTGTCCCAAGAAGAAGTGGCGGCGGACAATTCTGAATCTGCAGGCTTACCTACCACACCAATCCGAAAGCTGTCGAGAAATCGAGGTTTGGTTATGCAATCTGTTGTTGAAGATTCTCCTGACAATGGCTCTCAATCAGGAGCAACTGCAATTTTGAGGGGCAAGAGAAAGCTCAGTCAAGGTTGGGTTAATTAGAGCTCTGAAAGCTACCAAATGTGAATGCCTCTTTTTGTTCTGTGAGTTGTATATTTAACGGTTGCCTTGTAAATTGGTAATTAGGTTCTAAGAGTAAATGATCGCCAAATTTAACTTTTATAACAGAGTTTCAATTAAGTTGCAATGATGATTGCAAAGTTCCATACCTTTTTGGGTAGCTTAGATCAAGCTCCAATCATTTT

Coding sequence (CDS)

ATGAGGCCTGTCAAACGCGAACCCACCGTTAGCACCCGCAGTAATCGAAATAGAGGCGGCGACGGCGGTGGCGGTGGCGGTGGCGGCGCTGTTGACGGTGCGAGTTTAAGGGATAGTAAGCGGGAGAGGGTTGCCCGGGAGAGAGAGAACTCCGGAGAGGAGAATATGAATGAAGAAGTGGGTCAGCAGGATTTGACGAAGAGGCGGGTCCTTCGCTCTCGGTACCTCGCGGTTATTCATGAGATTAGTGAAAGAAGAGAGGATTTGTCGAAGGATTTGGATAAGTTTAATGTTATCATCAATGAAGTGGAAAAGTTGCACGAGCAAGTTCAGAAGCCAAGGGAACAAGTTGCAGATGCAGAAGCTTTGCAAGATATTGCCAACTCCCTTGTGACCTCTATTAGGTCCCAGTCAAATGAAGGTGTTACTCCATCAGATTTTGTTAGTTGTCTACTCAGAGAGTTTGCTGACATTAACGGTAGTATAAACTCTGAAGAACAAGACCTGGTTTCTGTCAATTGGAAAGATATTGGACTCAGTGTTTCGTCTATTTTCATGAATGGTTATGGATGCCGCACCATGCTTGGACCTATGAGCAATCAGTTGAAGCAGCGAAAGACTAATAATGTTCCCAGGAAACGTGTCAGGCCTACTGAAAGTTCCAGACCTGCAGAGGTTGAAGATAATGGATCCGAACCAAAAACAGATACAGATAAGAACATGGGCATCATGTTTGGAATTTTGAGGAGGAATAAAACCGTTAAACTAGAACATTTGATATTGAATAGAAAGTCTTTCGGACAGACAGTGGAGAATTTATTTGCTCTATCCTTTCTGGTTAAGGATGGTCGAGCTGAGATAACTATTGATAAAAATGGTTCTCATTTTGTGTCACCGAAGAATGCCCCTGCTCATAGTGCCATTATGTCACATGAAGTTAATTACAGTCATTTTGTATTCAGATTCGATTACAAGGATTGGAAGCCAATGGTGGATATGGTACCAGTTGGAGAGGAGCTGATGCCCCACAGGACCTTTCTGAACTCAGTGCAGGTGTCCCAAGAAGAAGTGGCGGCGGACAATTCTGAATCTGCAGGCTTACCTACCACACCAATCCGAAAGCTGTCGAGAAATCGAGGTTTGGTTATGCAATCTGTTGTTGAAGATTCTCCTGACAATGGCTCTCAATCAGGAGCAACTGCAATTTTGAGGGGCAAGAGAAAGCTCAGTCAAGGTTGGGTTAATTAG

Protein sequence

MRPVKREPTVSTRSNRNRGGDGGGGGGGGAVDGASLRDSKRERVARERENSGEENMNEEVGQQDLTKRRVLRSRYLAVIHEISERREDLSKDLDKFNVIINEVEKLHEQVQKPREQVADAEALQDIANSLVTSIRSQSNEGVTPSDFVSCLLREFADINGSINSEEQDLVSVNWKDIGLSVSSIFMNGYGCRTMLGPMSNQLKQRKTNNVPRKRVRPTESSRPAEVEDNGSEPKTDTDKNMGIMFGILRRNKTVKLEHLILNRKSFGQTVENLFALSFLVKDGRAEITIDKNGSHFVSPKNAPAHSAIMSHEVNYSHFVFRFDYKDWKPMVDMVPVGEELMPHRTFLNSVQVSQEEVAADNSESAGLPTTPIRKLSRNRGLVMQSVVEDSPDNGSQSGATAILRGKRKLSQGWVN*
BLAST of Cucsa.143010 vs. Swiss-Prot
Match: NSE4A_ARATH (Non-structural maintenance of chromosomes element 4 homolog A OS=Arabidopsis thaliana GN=NSE4A PE=2 SV=1)

HSP 1 Score: 365.5 bits (937), Expect = 7.5e-100
Identity = 205/404 (50.74%), Postives = 273/404 (67.57%), Query Frame = 1

Query: 13  RSNRNRGGDGGGGGGGGAVDGASLRDSKRERVARERENS--GEENMNEEVGQQDLTKRRV 72
           R    R  +  GG      +   LR  K+E+  +   +S   +E    +  +Q ++ RR+
Sbjct: 2   RKTVKRESEATGGKREADDEPEKLRSVKKEKQRKTEADSVRPDEPPPPQEEEQGISDRRI 61

Query: 73  LRSRYLAVIHEISERREDLSK-DLDKFNVIINEVEKLHEQVQKPREQVADAEALQDIANS 132
           LRS+YL++ +EI++ ++DL K D DKF+ IIN VE LH+QV+KPREQ+ADAEAL DIAN+
Sbjct: 62  LRSKYLSLQNEINDCKDDLMKIDSDKFSRIINAVENLHQQVRKPREQIADAEALLDIANT 121

Query: 133 LVTSIRSQSNEGVTPSDFVSCLLREFADINGSINSEEQDLVSVNWKDIGLSVSSIFMNGY 192
           L++S++SQS  GV+P++FV+ L+  F   +  I+++E   VS+ WKD+G +V S  +   
Sbjct: 122 LMSSVKSQSAHGVSPAEFVNALISGFGQGSLGIDTDETAQVSLKWKDLGFAVCSTVLVSC 181

Query: 193 GCRTMLGPMSNQLKQRKTNNVPRKRVRPTESSRPAEVEDNGSEPKTDTDKNMGIMFGILR 252
           GC TMLGPM  +LKQRK     RKR +P E  RP EV+D+ SE KTDTDKNM IMF IL 
Sbjct: 182 GCSTMLGPMDTELKQRK-RAPNRKRTKPGEGVRPDEVDDSQSEEKTDTDKNMTIMFNILG 241

Query: 253 RNKTVKLEHLILNRKSFGQTVENLFALSFLVKDGRAEITIDKNGSHFVSPKNAPAHSAIM 312
           + K V+LE+L+LNR+SF QTVENLFALSFL KDGR EI +DK+GSHF  P+NAP  + +M
Sbjct: 242 KKKRVQLENLVLNRRSFAQTVENLFALSFLAKDGRVEIIVDKSGSHFAMPRNAPDANVVM 301

Query: 313 SHEVNYSHFVFRFDYKDWKPMVDMVPVGEELMPHR-TFLNSVQVSQEEVAADNSESAGLP 372
           S EV Y+HFVFR D+KDWK M +MVP+GEELMPHR T + S         A    +    
Sbjct: 302 SGEVIYNHFVFRLDFKDWKLMSEMVPLGEELMPHRQTAVASSSCPAASAPASADFTQDTQ 361

Query: 373 TTPIRKLSRNRGLVMQ--SVVEDSPDNGSQSGATAILRGKRKLS 411
           TTPIRKLSRNRGLV+Q  +VVED+PD   + G     R KR+L+
Sbjct: 362 TTPIRKLSRNRGLVVQEETVVEDTPD---KEGDGTRRRCKRRLT 401

BLAST of Cucsa.143010 vs. Swiss-Prot
Match: NSE4B_ARATH (Non-structural maintenance of chromosomes element 4 homolog B OS=Arabidopsis thaliana GN=NSE4B PE=2 SV=1)

HSP 1 Score: 338.6 bits (867), Expect = 9.8e-92
Identity = 175/337 (51.93%), Postives = 241/337 (71.51%), Query Frame = 1

Query: 58  EEVGQQDLTKRRVLRSRYLAVIHEISERREDLSK-DLDKFNVIINEVEKLHEQVQKPREQ 117
           +E  +Q +  RR +RS+YLA+ H+I + ++DL+K D +KFN IINEVE LH++V+KPREQ
Sbjct: 52  QEEEEQGIADRRAIRSQYLALTHKIKDAKDDLTKIDSNKFNSIINEVENLHQKVRKPREQ 111

Query: 118 VADAEALQDIANSLVTSIRSQS-NEGVTPSDFVSCLLREFADINGSINSEEQDLVSVNWK 177
           +ADAEAL D+ NS+V+S++SQS + GV+P++FV+ L+  F   +  I+++E   VS+ WK
Sbjct: 112 IADAEALLDLTNSVVSSVKSQSAHGGVSPAEFVNALINGFGKTSLRIDADENTQVSMKWK 171

Query: 178 DIGLSVSSIFMNGYGCRTMLGPMSNQLKQRKTNNVPRKRVRPTESSRPAEVEDNGSEPKT 237
           D+G +V S  +   GC TM+GPM +++KQRK+    RKR +P    +P EV+D  +E K+
Sbjct: 172 DLGFTVCSTVLVSCGCTTMMGPMYSEMKQRKSRVGNRKRTKPGAGVKPEEVDDTEAEKKS 231

Query: 238 DTDKNMGIMFGILRRNKTVKLEHLILNRKSFGQTVENLFALSFLVKDGRAEITIDKNGSH 297
           DTD NM +MF ILR+NK VK+E+L+LNRKSF QT EN+FALSFLVKDGR EIT+D NGSH
Sbjct: 232 DTDNNMAVMFNILRKNKRVKIENLVLNRKSFAQTAENMFALSFLVKDGRVEITVDNNGSH 291

Query: 298 FVSPKNAPAHSAIMSHEVNYSHFVFRFDYKDWKPMVDMVPVGEELMPHRTFLNSVQVSQE 357
           FV P+NAPA + ++S EV Y+HFV RFDYKDW+PM  MV VGEELMP    L   +V+Q+
Sbjct: 292 FVEPRNAPAANLVLSGEVAYNHFVLRFDYKDWEPMSKMVAVGEELMP----LRETKVAQD 351

Query: 358 EVAADNSESAGLPTTPIRKLSRNRGLVMQ--SVVEDS 391
            +                +LSR +G V+Q  +VVEDS
Sbjct: 352 SI----------------ELSRKQGSVIQEETVVEDS 368

BLAST of Cucsa.143010 vs. Swiss-Prot
Match: NSE4A_BOVIN (Non-structural maintenance of chromosomes element 4 homolog A OS=Bos taurus GN=NSMCE4A PE=2 SV=1)

HSP 1 Score: 84.7 bits (208), Expect = 2.6e-15
Identity = 73/316 (23.10%), Postives = 140/316 (44.30%), Query Frame = 1

Query: 47  ERENSGEENMNEEVGQQ--DLTKRRVLRSRYLAVIHEISERREDLSKDLDKFNVIINEVE 106
           E  +SG+E ++    ++  D +  R +R +Y A+I+ + + RED+    DK   ++ E  
Sbjct: 59  EPSDSGDEMIDPASLEEETDPSLCRQIRHQYRALINSVQQNREDILNASDKLTEVLEEAN 118

Query: 107 KLHEQVQKPREQVADAEAL---QDIANSLVTSIRSQSNEGVTPSDFVSCLLREFAD---- 166
            L   V + RE V DA  L    D+       +RS  N       +V  LL         
Sbjct: 119 TLFNGVSRAREAVLDAHFLVLASDLGKEKAKQLRSDLN-SFDMLRYVETLLTHMGVNPLE 178

Query: 167 ----INGSINSEEQDLVSVNWKDIGLSVSSIFMNGYGCRTMLGPMSNQLKQRKTNNVPRK 226
               I    +S+ + +V  +WK  G +  + F   +    +LG +  +    K      +
Sbjct: 179 AEELIRDEDSSDLEFIVYDSWKISGKTAENTFNKTHTFHFLLGSIQGECPVPKPRIERPR 238

Query: 227 RVRPTESSR--PAEVEDNGSEPKTDTDKNMGIMFGILRR------NKTVKLEHLILNRKS 286
           +VR  E  +  PA+++      +  T+K +  + G+L+       +  +     +++  S
Sbjct: 239 KVRMIEEQQAMPAQLKRMEESHQEATEKEVERILGLLQTYFQEDPDTPMSFFDFVVDPHS 298

Query: 287 FGQTVENLFALSFLVKDGRAEITIDKNGSHFVSPKNAPAHSAIMSHEVNYSHFVFRFDYK 342
           F +TVEN+F +SF+++DG A I +D++    + P N    S   +   N +  +    Y+
Sbjct: 299 FPRTVENIFHVSFIIRDGFARIRLDRDRLPVIEPVNINEESGGNTQIRNQA--IIALSYR 358

BLAST of Cucsa.143010 vs. Swiss-Prot
Match: NSE4A_HUMAN (Non-structural maintenance of chromosomes element 4 homolog A OS=Homo sapiens GN=NSMCE4A PE=1 SV=2)

HSP 1 Score: 82.0 bits (201), Expect = 1.7e-14
Identity = 72/319 (22.57%), Postives = 137/319 (42.95%), Query Frame = 1

Query: 47  ERENSGEENMNEEV--GQQDLTKRRVLRSRYLAVIHEISERREDLSKDLDKFNVIINEVE 106
           E  +SG+E M+      + D    R +R +Y A+I+ + + RED+    DK   ++ E  
Sbjct: 59  EPSDSGDEMMDPASLEAEADQGLCRQIRHQYRALINSVQQNREDILNAGDKLTEVLEEAN 118

Query: 107 KLHEQVQKPREQVADAEALQDIANSLVTSIRSQSNEGVTPSD---FVSCLLREFA----D 166
            L  +V + RE V DA  L  +A+ L      Q    ++  D   +V  LL        +
Sbjct: 119 TLFNEVSRAREAVLDAHFLV-LASDLGKEKAKQLRSDLSSFDMLRYVETLLTHMGVNPLE 178

Query: 167 INGSINSEE----QDLVSVNWKDIGLSVSSIFMNGYGCRTMLG--------PMSNQLKQR 226
               I  E+    + +V  +WK  G +  + F   +    +LG        P     + R
Sbjct: 179 AEELIRDEDSPDFEFIVYDSWKITGRTAENTFNKTHTFHFLLGSIYGECPVPKPRVDRPR 238

Query: 227 KTNNVPRKRVRPTESSRPAEVEDNGSEPKTDTDKNMGIMFGILRRNKTVKLE--HLILNR 286
           K   +  +R  P +  R  E     +E   + ++ +G++    R +    +     +++ 
Sbjct: 239 KVPVIQEERAMPAQLRRMEESHQEATE--KEVERILGLLQTYFREDPDTPMSFFDFVVDP 298

Query: 287 KSFGQTVENLFALSFLVKDGRAEITIDKNGSHFVSPKNAPAHSAIMSHEVNY-SHFVFRF 342
            SF +TVEN+F +SF+++DG A I +D++    + P +    +    H     +  +   
Sbjct: 299 HSFPRTVENIFHVSFIIRDGFARIRLDQDRLPVIEPVSINEENEGFEHNTQVRNQGIIAL 358

BLAST of Cucsa.143010 vs. Swiss-Prot
Match: NSE4_SCHPO (Non-structural maintenance of chromosome element 4 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) GN=nse4 PE=1 SV=1)

HSP 1 Score: 75.1 bits (183), Expect = 2.0e-12
Identity = 65/283 (22.97%), Postives = 126/283 (44.52%), Query Frame = 1

Query: 68  RRVLRSRYLAVIHEISERREDL-SKDLDKFNVIINEVEKLHEQVQKPREQVADAEALQDI 127
           +R LR RY  +I+++ E R +L  ++ +     I     L   V  P E   DA  L   
Sbjct: 6   KRDLRKRYRNLINKVQESRLELVDEENNNLYETITTANDLFSSVDAPTEATLDALLLTKT 65

Query: 128 ANSLVTSIRSQSNEGVTPSDFVSCL---LREFADINGS-INSEEQDLVSVNWKDIGLSVS 187
            +  + SI+++      P   +      +++F +   S  N      +   W  +G   S
Sbjct: 66  VD--LASIKARQLHIGRPKFNIELFTKNIKQFLNYPTSHSNVTRIQEIDTAWSRLGKLAS 125

Query: 188 SIFMNGYGCRTMLGPMSNQLKQRKTNNVPRKRVRPTESSRPAEV-EDNGSEPKTDTDKNM 247
           +          M+GP+S + K+R      R +  P   ++P  + E N +  + +T KN+
Sbjct: 126 NCEKQPASLNLMVGPLSFRKKERNIQRRERLQKAPNVLTQPTMLNERNITTQENNTTKNV 185

Query: 248 GIMFGILRRNKTVKLEHLILNRKSFGQTVENLFALSFLVKDGRAEITIDKNGSHFVSPKN 307
             +  +L+ ++ V     I N +S+ QTVENLF +SFL K+G+A +  +++G   +  + 
Sbjct: 186 LHISRLLQAHQPVNFLKFITNPQSYPQTVENLFYVSFLFKEGKAALVENESGILMLETRI 245

Query: 308 APAHSAIMSHEVNYSHFVFRFDYKDWKPMVDMVPVGEELMPHR 345
            P    +++ E+     V       ++ ++    + E ++P R
Sbjct: 246 PPTDDQVVAGEIRNIQLVLDMTMDLYENIIKEYNIKESIIPTR 286

BLAST of Cucsa.143010 vs. TrEMBL
Match: A0A0A0LZJ0_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_1G605680 PE=4 SV=1)

HSP 1 Score: 821.6 bits (2121), Expect = 4.3e-235
Identity = 415/415 (100.00%), Postives = 415/415 (100.00%), Query Frame = 1

Query: 1   MRPVKREPTVSTRSNRNRGGDGGGGGGGGAVDGASLRDSKRERVARERENSGEENMNEEV 60
           MRPVKREPTVSTRSNRNRGGDGGGGGGGGAVDGASLRDSKRERVARERENSGEENMNEEV
Sbjct: 1   MRPVKREPTVSTRSNRNRGGDGGGGGGGGAVDGASLRDSKRERVARERENSGEENMNEEV 60

Query: 61  GQQDLTKRRVLRSRYLAVIHEISERREDLSKDLDKFNVIINEVEKLHEQVQKPREQVADA 120
           GQQDLTKRRVLRSRYLAVIHEISERREDLSKDLDKFNVIINEVEKLHEQVQKPREQVADA
Sbjct: 61  GQQDLTKRRVLRSRYLAVIHEISERREDLSKDLDKFNVIINEVEKLHEQVQKPREQVADA 120

Query: 121 EALQDIANSLVTSIRSQSNEGVTPSDFVSCLLREFADINGSINSEEQDLVSVNWKDIGLS 180
           EALQDIANSLVTSIRSQSNEGVTPSDFVSCLLREFADINGSINSEEQDLVSVNWKDIGLS
Sbjct: 121 EALQDIANSLVTSIRSQSNEGVTPSDFVSCLLREFADINGSINSEEQDLVSVNWKDIGLS 180

Query: 181 VSSIFMNGYGCRTMLGPMSNQLKQRKTNNVPRKRVRPTESSRPAEVEDNGSEPKTDTDKN 240
           VSSIFMNGYGCRTMLGPMSNQLKQRKTNNVPRKRVRPTESSRPAEVEDNGSEPKTDTDKN
Sbjct: 181 VSSIFMNGYGCRTMLGPMSNQLKQRKTNNVPRKRVRPTESSRPAEVEDNGSEPKTDTDKN 240

Query: 241 MGIMFGILRRNKTVKLEHLILNRKSFGQTVENLFALSFLVKDGRAEITIDKNGSHFVSPK 300
           MGIMFGILRRNKTVKLEHLILNRKSFGQTVENLFALSFLVKDGRAEITIDKNGSHFVSPK
Sbjct: 241 MGIMFGILRRNKTVKLEHLILNRKSFGQTVENLFALSFLVKDGRAEITIDKNGSHFVSPK 300

Query: 301 NAPAHSAIMSHEVNYSHFVFRFDYKDWKPMVDMVPVGEELMPHRTFLNSVQVSQEEVAAD 360
           NAPAHSAIMSHEVNYSHFVFRFDYKDWKPMVDMVPVGEELMPHRTFLNSVQVSQEEVAAD
Sbjct: 301 NAPAHSAIMSHEVNYSHFVFRFDYKDWKPMVDMVPVGEELMPHRTFLNSVQVSQEEVAAD 360

Query: 361 NSESAGLPTTPIRKLSRNRGLVMQSVVEDSPDNGSQSGATAILRGKRKLSQGWVN 416
           NSESAGLPTTPIRKLSRNRGLVMQSVVEDSPDNGSQSGATAILRGKRKLSQGWVN
Sbjct: 361 NSESAGLPTTPIRKLSRNRGLVMQSVVEDSPDNGSQSGATAILRGKRKLSQGWVN 415

BLAST of Cucsa.143010 vs. TrEMBL
Match: M5VYK3_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa006782mg PE=4 SV=1)

HSP 1 Score: 436.4 bits (1121), Expect = 3.9e-119
Identity = 236/386 (61.14%), Postives = 288/386 (74.61%), Query Frame = 1

Query: 28  GGAVDGASLRDSKRERVARERENSGE-ENMNEEVGQQDLTKRRVLRSRYLAVIHEISERR 87
           G   D   LR  KRERV     N+G  EN ++   +QD  +RR LRS YLAV + ISE+R
Sbjct: 15  GDVDDAEQLRAVKRERV---NANAGRVENESDLPSEQDSVQRRTLRSDYLAVKNLISEKR 74

Query: 88  EDLS-KDLDKFNVIINEVEKLHEQVQKPREQVADAEALQDIANSLVTSIRSQSNEGVTPS 147
           +DL   + ++F +IIN+VEKLHEQVQKPREQVADAEAL DI N+L TS++SQS+ G++PS
Sbjct: 75  DDLMCPESERFCLIINKVEKLHEQVQKPREQVADAEALLDITNTLWTSVKSQSSAGISPS 134

Query: 148 DFVSCLLREFADINGSINSEEQDLVSVNWKDIGLSVSSIFMNGYGCRTMLGPMSNQLKQR 207
           DFV+C+L  F  +N S+ S+E   VS+ WKDIGL+V+ IF   +GC TMLGPM+ +LKQR
Sbjct: 135 DFVTCVLNNFKQLNSSLASQEDAPVSIKWKDIGLAVAPIFKRAHGCCTMLGPMNTELKQR 194

Query: 208 KTNNVPRKRVRPTESSRPAEVEDNGSEPKTDTDKNMGIMFGILRRNKTVKLEHLILNRKS 267
           K   V  K  +PT + RP E++D   E KTDTDKNM  MF ILRRNK V+LE LILNRKS
Sbjct: 195 KAV-VRSKHAKPTTTDRPDEIDDTQGEEKTDTDKNMSTMFDILRRNKRVRLEGLILNRKS 254

Query: 268 FGQTVENLFALSFLVKDGRAEITIDKNGSHFVSPKNAPAHSAIMSHEVNYSHFVFRFDYK 327
           F QTVENLFALSFLVKDGRAEIT+D NGSH VSP+NAPA + + S EV Y HFVFRFD+K
Sbjct: 255 FAQTVENLFALSFLVKDGRAEITVDANGSHLVSPRNAPAANLVASREVVYDHFVFRFDFK 314

Query: 328 DWKPMVDMVPVGEELMPHRTFLNSVQVSQEEVAADNSESAGLPTTPIRKLSRNRGLVMQ- 387
           DWK M DM+PVGEELMPHR+  NS+  SQEE AA +S++A LPTTPIRKLSRNRG ++Q 
Sbjct: 315 DWKLMKDMLPVGEELMPHRSPPNSLPASQEEPAAYSSQTA-LPTTPIRKLSRNRGRLVQE 374

Query: 388 -SVVEDSPDNGSQSGATAILRGKRKL 410
            SVVE+SP+N   +G   + R KRKL
Sbjct: 375 ESVVEESPENDDAAGTNVVRRSKRKL 395

BLAST of Cucsa.143010 vs. TrEMBL
Match: A0A059D627_EUCGR (Uncharacterized protein OS=Eucalyptus grandis GN=EUGRSUZ_B02593 PE=4 SV=1)

HSP 1 Score: 426.4 bits (1095), Expect = 4.0e-116
Identity = 241/414 (58.21%), Postives = 293/414 (70.77%), Query Frame = 1

Query: 4   VKRE---PTVSTRSNRNRGGDGGGGGGGGAVDGASLRDSKRERVARERENSGEENMNEEV 63
           VKRE     VS+      G    G GGG A +   LR +KRER +R RE   E+  + + 
Sbjct: 68  VKRERLGKAVSSDGGAGVGSKRKGAGGGAAPE--QLRAAKRERSSRNRE---EKEPSSQA 127

Query: 64  GQQDLTKRRVLRSRYLAVIHEISERREDLSK-DLDKFNVIINEVEKLHEQVQKPREQVAD 123
            +QD ++RRVLRS YLAV + I++ R+DL + D DKF  II EVE L+E VQKPREQVAD
Sbjct: 128 TEQDDSERRVLRSHYLAVKNLINDERDDLLRADSDKFATIITEVENLYELVQKPREQVAD 187

Query: 124 AEALQDIANSLVTSIRSQSNEGVTPSDFVSCLLREFADINGSINSEEQDLVSVNWKDIGL 183
           AEAL  IAN+LV+S++S S++G+TPSDFV+CLL+EF    G +   E D V   WKD+G+
Sbjct: 188 AEALLGIANTLVSSVKSYSSDGITPSDFVNCLLKEFGQPMGGLGIGENDQVPFIWKDLGV 247

Query: 184 SVSSIFMNGYGCRTMLGPMSNQLKQRKTNNVPRKRVRPTESSRPAEVEDNGSEPKTDTDK 243
            VS IF N  GC TMLGPM+ QLKQRK   V R+  RPTE+SRP E++D G E KTDTDK
Sbjct: 248 RVSPIFRNFQGCCTMLGPMNTQLKQRKVT-VHRRHTRPTENSRPEEIDDAGVEKKTDTDK 307

Query: 244 NMGIMFGILRRNKTVKLEHLILNRKSFGQTVENLFALSFLVKDGRAEITIDKNGSHFVSP 303
           NM  MF ILRR K V L+ LILNR+SF QTVENLFALSFLVKDGRAE+T+D+ G HFVSP
Sbjct: 308 NMSTMFEILRRKKRVGLDSLILNRQSFAQTVENLFALSFLVKDGRAEVTVDEKGYHFVSP 367

Query: 304 KNAPAHSAIMSHEVNYSHFVFRFDYKDWKPMVDMVPVGEELMPHRTFLNSVQVSQEEVAA 363
           KNAP  S ++S EV Y HFVFRFDY+DWK M+  +P GEELMPHR        SQ ++ +
Sbjct: 368 KNAPVSSLVLSGEVAYGHFVFRFDYRDWKMMISTLPHGEELMPHREQQTCSAASQADLVS 427

Query: 364 DNSESAGLPTTPIRKLSRNRGLVMQ--SVVEDSP--DNGSQSGATAILRGKRKL 410
            NS+S  LPTTPIRKL+RNRGLV+Q  SVVEDSP  D+ + S A AI + KRKL
Sbjct: 428 CNSQSV-LPTTPIRKLTRNRGLVIQEESVVEDSPVVDDDTSSRANAIRKCKRKL 474

BLAST of Cucsa.143010 vs. TrEMBL
Match: F6HLJ8_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_08s0007g06380 PE=4 SV=1)

HSP 1 Score: 421.0 bits (1081), Expect = 1.7e-114
Identity = 230/380 (60.53%), Postives = 277/380 (72.89%), Query Frame = 1

Query: 36  LRDSKRERVARERENSGEENMNEEVGQQDLTKRRVLRSRYLAVIHEISERREDLSK-DLD 95
           +R  KRE  +  R N+ E   +   G Q +T+RRVLRSRYLAV ++I + RED++K D D
Sbjct: 2   VRAVKREVGSSSRANANEAGADNS-GHQGVTERRVLRSRYLAVKNQICDGREDITKVDSD 61

Query: 96  KFNVIINEVEKLHEQVQKPREQVADAEALQDIANSLVTSIRSQSNEGVTPSDFVSCLLRE 155
           KF  IINEVE LH+QVQKPREQVADAEAL DI N+LVTS+++  NEG+TPSDFVSCLL+E
Sbjct: 62  KFKSIINEVESLHQQVQKPREQVADAEALLDITNTLVTSVKAHGNEGITPSDFVSCLLQE 121

Query: 156 FADINGSINSEEQDLVSVNWKDIGLSVSSIFMNGYGCRTMLGPMSNQLKQRKTNNVPRKR 215
           F    G   S E    S+ WKDIGL VS IF    GC TMLGPM+ ++KQRK   V  KR
Sbjct: 122 FGQNPGVSTSAEDAGNSIVWKDIGLVVSHIFKRASGCCTMLGPMNTEMKQRKPV-VHNKR 181

Query: 216 VRPTESSRPAEVEDNGSEPKTDTDKNMGIMFGILRRNKTVKLEHLILNRKSFGQTVENLF 275
           VRPTES+RP E++D   E K+DTDKNM IMF ILR+N+ V+LE+LILNR SF QTVENLF
Sbjct: 182 VRPTESARPEELDDTVVEEKSDTDKNMSIMFDILRKNRRVRLENLILNRNSFAQTVENLF 241

Query: 276 ALSFLVKDGRAEITIDKNGSHFVSPKNAPAHSAIMSHEVNYSHFVFRFDYKDWKPMVDMV 335
           ALSFLVKDGRAEIT+D  G H VSP+NAPA +A++S EV Y+HFVFRFD+KDWK M   V
Sbjct: 242 ALSFLVKDGRAEITVDGRGCHLVSPRNAPAANAVVSGEVCYNHFVFRFDFKDWKLMATSV 301

Query: 336 PVGEELMPHRTFLNSVQVSQEEVAADNSESAGLPTTPIRKLSRNRGLVM--QSVVEDSPD 395
             GEELMP R  +N    SQ +  A+  E+A  PTTPIRKLSRNRGLV+  Q+VVEDSP+
Sbjct: 302 GAGEELMPQRNSVNLPNNSQTDSVAEECEAAAAPTTPIRKLSRNRGLVLQEQAVVEDSPE 361

Query: 396 NGSQSGATAILR---GKRKL 410
           +       A++R   GKRKL
Sbjct: 362 SDDAGTRAAVIRKGKGKRKL 379

BLAST of Cucsa.143010 vs. TrEMBL
Match: A0A061GSH5_THECC (Nse4, component of Smc5/6 DNA repair complex, putative isoform 1 OS=Theobroma cacao GN=TCM_040402 PE=4 SV=1)

HSP 1 Score: 421.0 bits (1081), Expect = 1.7e-114
Identity = 224/373 (60.05%), Postives = 286/373 (76.68%), Query Frame = 1

Query: 41  RERVARE-RENSGEENMNEEVGQQDLTKRRVLRSRYLAVIHEISERREDLSK-DLDKFNV 100
           R RVAR  R +S +   ++   Q+D   RR+LRS+YLAV+ +I++ R+++SK D +KFN+
Sbjct: 21  RTRVARPARHDSADVESDQLSRQEDAANRRLLRSKYLAVMTKINDARDEISKVDSNKFNI 80

Query: 101 IINEVEKLHEQVQKPREQVADAEALQDIANSLVTSIRSQSNEGVTPSDFVSCLLREFADI 160
           IINEV+ LH+QV KPREQVADAEAL DIAN+L TS++S S EGV+ +D+V+CL+REF   
Sbjct: 81  IINEVDNLHQQVSKPREQVADAEALLDIANTLATSVKSISCEGVSLADYVNCLVREFGKS 140

Query: 161 NGSINSEEQDLVSVNWKDIGLSVSSIFMNGYGCRTMLGPMSNQLKQRKTNNVPRKRVRPT 220
           N S++++E + +SVNWKDIG++VS  F    G  TMLGPM N+LKQRK+    ++ +RPT
Sbjct: 141 NRSLDTQENEQISVNWKDIGMAVSPFFTTCKGICTMLGPMKNELKQRKSIVTRKRAIRPT 200

Query: 221 ESSRPAEVEDNGSEPKTDTDKNMGIMFGILRRNKTVKLEHLILNRKSFGQTVENLFALSF 280
           +++RP EV+D G+E KTDTDKNM  MF ILRR + VKLE LILNR SF QTVENLFALSF
Sbjct: 201 DTARPEEVDDTGAEEKTDTDKNMATMFEILRRKRQVKLESLILNRSSFAQTVENLFALSF 260

Query: 281 LVKDGRAEITIDKNGSHFVSPKNAPAHSAIMSHEVNYSHFVFRFDYKDWKPMVDMVPVGE 340
           LVKDGRAEI +DK+GSH VSPKNAP   ++ + EV YSHFVFRFD+KDWK M+  VPVGE
Sbjct: 261 LVKDGRAEIVVDKSGSHIVSPKNAPGTMSVTTGEVTYSHFVFRFDFKDWKLMMSGVPVGE 320

Query: 341 ELMPHRTFLNSVQVSQEEVAADNSESAGLPTTPIRKLSRNRGLVMQ-SVVEDSPDNGSQS 400
           ELMPHR   +S+ +S+ E AA+NSE A L TTPIRKLSRNRGLV+Q S+VEDSP+    S
Sbjct: 321 ELMPHREESHSI-LSKAESAANNSEGA-LTTTPIRKLSRNRGLVLQESIVEDSPEIDDGS 380

Query: 401 GATAILRGKRKLS 411
               I R +RKL+
Sbjct: 381 KGPGIRRCRRKLN 391

BLAST of Cucsa.143010 vs. TAIR10
Match: AT1G51130.1 (AT1G51130.1 Nse4, component of Smc5/6 DNA repair complex)

HSP 1 Score: 365.5 bits (937), Expect = 4.2e-101
Identity = 205/404 (50.74%), Postives = 273/404 (67.57%), Query Frame = 1

Query: 13  RSNRNRGGDGGGGGGGGAVDGASLRDSKRERVARERENS--GEENMNEEVGQQDLTKRRV 72
           R    R  +  GG      +   LR  K+E+  +   +S   +E    +  +Q ++ RR+
Sbjct: 2   RKTVKRESEATGGKREADDEPEKLRSVKKEKQRKTEADSVRPDEPPPPQEEEQGISDRRI 61

Query: 73  LRSRYLAVIHEISERREDLSK-DLDKFNVIINEVEKLHEQVQKPREQVADAEALQDIANS 132
           LRS+YL++ +EI++ ++DL K D DKF+ IIN VE LH+QV+KPREQ+ADAEAL DIAN+
Sbjct: 62  LRSKYLSLQNEINDCKDDLMKIDSDKFSRIINAVENLHQQVRKPREQIADAEALLDIANT 121

Query: 133 LVTSIRSQSNEGVTPSDFVSCLLREFADINGSINSEEQDLVSVNWKDIGLSVSSIFMNGY 192
           L++S++SQS  GV+P++FV+ L+  F   +  I+++E   VS+ WKD+G +V S  +   
Sbjct: 122 LMSSVKSQSAHGVSPAEFVNALISGFGQGSLGIDTDETAQVSLKWKDLGFAVCSTVLVSC 181

Query: 193 GCRTMLGPMSNQLKQRKTNNVPRKRVRPTESSRPAEVEDNGSEPKTDTDKNMGIMFGILR 252
           GC TMLGPM  +LKQRK     RKR +P E  RP EV+D+ SE KTDTDKNM IMF IL 
Sbjct: 182 GCSTMLGPMDTELKQRK-RAPNRKRTKPGEGVRPDEVDDSQSEEKTDTDKNMTIMFNILG 241

Query: 253 RNKTVKLEHLILNRKSFGQTVENLFALSFLVKDGRAEITIDKNGSHFVSPKNAPAHSAIM 312
           + K V+LE+L+LNR+SF QTVENLFALSFL KDGR EI +DK+GSHF  P+NAP  + +M
Sbjct: 242 KKKRVQLENLVLNRRSFAQTVENLFALSFLAKDGRVEIIVDKSGSHFAMPRNAPDANVVM 301

Query: 313 SHEVNYSHFVFRFDYKDWKPMVDMVPVGEELMPHR-TFLNSVQVSQEEVAADNSESAGLP 372
           S EV Y+HFVFR D+KDWK M +MVP+GEELMPHR T + S         A    +    
Sbjct: 302 SGEVIYNHFVFRLDFKDWKLMSEMVPLGEELMPHRQTAVASSSCPAASAPASADFTQDTQ 361

Query: 373 TTPIRKLSRNRGLVMQ--SVVEDSPDNGSQSGATAILRGKRKLS 411
           TTPIRKLSRNRGLV+Q  +VVED+PD   + G     R KR+L+
Sbjct: 362 TTPIRKLSRNRGLVVQEETVVEDTPD---KEGDGTRRRCKRRLT 401

BLAST of Cucsa.143010 vs. TAIR10
Match: AT3G20760.1 (AT3G20760.1 Nse4, component of Smc5/6 DNA repair complex)

HSP 1 Score: 338.6 bits (867), Expect = 5.5e-93
Identity = 175/337 (51.93%), Postives = 241/337 (71.51%), Query Frame = 1

Query: 58  EEVGQQDLTKRRVLRSRYLAVIHEISERREDLSK-DLDKFNVIINEVEKLHEQVQKPREQ 117
           +E  +Q +  RR +RS+YLA+ H+I + ++DL+K D +KFN IINEVE LH++V+KPREQ
Sbjct: 52  QEEEEQGIADRRAIRSQYLALTHKIKDAKDDLTKIDSNKFNSIINEVENLHQKVRKPREQ 111

Query: 118 VADAEALQDIANSLVTSIRSQS-NEGVTPSDFVSCLLREFADINGSINSEEQDLVSVNWK 177
           +ADAEAL D+ NS+V+S++SQS + GV+P++FV+ L+  F   +  I+++E   VS+ WK
Sbjct: 112 IADAEALLDLTNSVVSSVKSQSAHGGVSPAEFVNALINGFGKTSLRIDADENTQVSMKWK 171

Query: 178 DIGLSVSSIFMNGYGCRTMLGPMSNQLKQRKTNNVPRKRVRPTESSRPAEVEDNGSEPKT 237
           D+G +V S  +   GC TM+GPM +++KQRK+    RKR +P    +P EV+D  +E K+
Sbjct: 172 DLGFTVCSTVLVSCGCTTMMGPMYSEMKQRKSRVGNRKRTKPGAGVKPEEVDDTEAEKKS 231

Query: 238 DTDKNMGIMFGILRRNKTVKLEHLILNRKSFGQTVENLFALSFLVKDGRAEITIDKNGSH 297
           DTD NM +MF ILR+NK VK+E+L+LNRKSF QT EN+FALSFLVKDGR EIT+D NGSH
Sbjct: 232 DTDNNMAVMFNILRKNKRVKIENLVLNRKSFAQTAENMFALSFLVKDGRVEITVDNNGSH 291

Query: 298 FVSPKNAPAHSAIMSHEVNYSHFVFRFDYKDWKPMVDMVPVGEELMPHRTFLNSVQVSQE 357
           FV P+NAPA + ++S EV Y+HFV RFDYKDW+PM  MV VGEELMP    L   +V+Q+
Sbjct: 292 FVEPRNAPAANLVLSGEVAYNHFVLRFDYKDWEPMSKMVAVGEELMP----LRETKVAQD 351

Query: 358 EVAADNSESAGLPTTPIRKLSRNRGLVMQ--SVVEDS 391
            +                +LSR +G V+Q  +VVEDS
Sbjct: 352 SI----------------ELSRKQGSVIQEETVVEDS 368

BLAST of Cucsa.143010 vs. NCBI nr
Match: gi|449462613|ref|XP_004149035.1| (PREDICTED: non-structural maintenance of chromosomes element 4 homolog A-like [Cucumis sativus])

HSP 1 Score: 821.6 bits (2121), Expect = 6.1e-235
Identity = 415/415 (100.00%), Postives = 415/415 (100.00%), Query Frame = 1

Query: 1   MRPVKREPTVSTRSNRNRGGDGGGGGGGGAVDGASLRDSKRERVARERENSGEENMNEEV 60
           MRPVKREPTVSTRSNRNRGGDGGGGGGGGAVDGASLRDSKRERVARERENSGEENMNEEV
Sbjct: 1   MRPVKREPTVSTRSNRNRGGDGGGGGGGGAVDGASLRDSKRERVARERENSGEENMNEEV 60

Query: 61  GQQDLTKRRVLRSRYLAVIHEISERREDLSKDLDKFNVIINEVEKLHEQVQKPREQVADA 120
           GQQDLTKRRVLRSRYLAVIHEISERREDLSKDLDKFNVIINEVEKLHEQVQKPREQVADA
Sbjct: 61  GQQDLTKRRVLRSRYLAVIHEISERREDLSKDLDKFNVIINEVEKLHEQVQKPREQVADA 120

Query: 121 EALQDIANSLVTSIRSQSNEGVTPSDFVSCLLREFADINGSINSEEQDLVSVNWKDIGLS 180
           EALQDIANSLVTSIRSQSNEGVTPSDFVSCLLREFADINGSINSEEQDLVSVNWKDIGLS
Sbjct: 121 EALQDIANSLVTSIRSQSNEGVTPSDFVSCLLREFADINGSINSEEQDLVSVNWKDIGLS 180

Query: 181 VSSIFMNGYGCRTMLGPMSNQLKQRKTNNVPRKRVRPTESSRPAEVEDNGSEPKTDTDKN 240
           VSSIFMNGYGCRTMLGPMSNQLKQRKTNNVPRKRVRPTESSRPAEVEDNGSEPKTDTDKN
Sbjct: 181 VSSIFMNGYGCRTMLGPMSNQLKQRKTNNVPRKRVRPTESSRPAEVEDNGSEPKTDTDKN 240

Query: 241 MGIMFGILRRNKTVKLEHLILNRKSFGQTVENLFALSFLVKDGRAEITIDKNGSHFVSPK 300
           MGIMFGILRRNKTVKLEHLILNRKSFGQTVENLFALSFLVKDGRAEITIDKNGSHFVSPK
Sbjct: 241 MGIMFGILRRNKTVKLEHLILNRKSFGQTVENLFALSFLVKDGRAEITIDKNGSHFVSPK 300

Query: 301 NAPAHSAIMSHEVNYSHFVFRFDYKDWKPMVDMVPVGEELMPHRTFLNSVQVSQEEVAAD 360
           NAPAHSAIMSHEVNYSHFVFRFDYKDWKPMVDMVPVGEELMPHRTFLNSVQVSQEEVAAD
Sbjct: 301 NAPAHSAIMSHEVNYSHFVFRFDYKDWKPMVDMVPVGEELMPHRTFLNSVQVSQEEVAAD 360

Query: 361 NSESAGLPTTPIRKLSRNRGLVMQSVVEDSPDNGSQSGATAILRGKRKLSQGWVN 416
           NSESAGLPTTPIRKLSRNRGLVMQSVVEDSPDNGSQSGATAILRGKRKLSQGWVN
Sbjct: 361 NSESAGLPTTPIRKLSRNRGLVMQSVVEDSPDNGSQSGATAILRGKRKLSQGWVN 415

BLAST of Cucsa.143010 vs. NCBI nr
Match: gi|659100485|ref|XP_008451119.1| (PREDICTED: non-structural maintenance of chromosomes element 4 homolog A [Cucumis melo])

HSP 1 Score: 770.4 bits (1988), Expect = 1.6e-219
Identity = 393/412 (95.39%), Postives = 399/412 (96.84%), Query Frame = 1

Query: 1   MRPVKREPTVSTRSNRNRGGDGGGGGGGGAVDGASLRDSKRERVARERENSGEENMNEEV 60
           MRPVKREPTVSTRS RNRGGDGGG    GAVDGASLRDSKRERVARERENSGEENM+EEV
Sbjct: 1   MRPVKREPTVSTRSKRNRGGDGGG----GAVDGASLRDSKRERVARERENSGEENMSEEV 60

Query: 61  GQQDLTKRRVLRSRYLAVIHEISERREDLSKDLDKFNVIINEVEKLHEQVQKPREQVADA 120
           GQQDLTKRRVLRSRYLAVIHEISERREDLSKDLDKFNVIINEVEKLHEQVQKPREQVADA
Sbjct: 61  GQQDLTKRRVLRSRYLAVIHEISERREDLSKDLDKFNVIINEVEKLHEQVQKPREQVADA 120

Query: 121 EALQDIANSLVTSIRSQSNEGVTPSDFVSCLLREFADINGSINSEEQDLVSVNWKDIGLS 180
           EALQDIANSLVTSIRSQSNEGVTPSDFVSCLLREFADINGSINSEEQ+LVSVNWKDIGLS
Sbjct: 121 EALQDIANSLVTSIRSQSNEGVTPSDFVSCLLREFADINGSINSEEQELVSVNWKDIGLS 180

Query: 181 VSSIFMNGYGCRTMLGPMSNQLKQRKTNNVPRKRVRPTESSRPAEVEDNGSEPKTDTDKN 240
           VSSIFMNGYGCRTMLGPMSNQLKQRKTNNVPRKR +PTESSRPAEVEDNGSEPKTDTDKN
Sbjct: 181 VSSIFMNGYGCRTMLGPMSNQLKQRKTNNVPRKRSKPTESSRPAEVEDNGSEPKTDTDKN 240

Query: 241 MGIMFGILRRNKTVKLEHLILNRKSFGQTVENLFALSFLVKDGRAEITIDKNGSHFVSPK 300
           MGIMFGILRRNKTVKLEHLILNRKSF QTVENLFALSFLVKDGRAEITIDKNGSHFVSPK
Sbjct: 241 MGIMFGILRRNKTVKLEHLILNRKSFAQTVENLFALSFLVKDGRAEITIDKNGSHFVSPK 300

Query: 301 NAPAHSAIMSHEVNYSHFVFRFDYKDWKPMVDMVPVGEELMPHRTFLNSVQVSQEEVAAD 360
           NAPAHSAIMSHEVNYSHFVFRFDYKDWKPMV+MVPVGEELMPHR FLNSV VS+EE  AD
Sbjct: 301 NAPAHSAIMSHEVNYSHFVFRFDYKDWKPMVNMVPVGEELMPHRNFLNSVPVSEEEATAD 360

Query: 361 NSESAGLPTTPIRKLSRNRGLVMQSVVEDSPDNGSQSGATAILRGKRKLSQG 413
           +SESA LPTTPIRKLSRNRGLVMQSVVEDSPDNGSQSG TAILRGKRKLSQG
Sbjct: 361 DSESASLPTTPIRKLSRNRGLVMQSVVEDSPDNGSQSGPTAILRGKRKLSQG 408

BLAST of Cucsa.143010 vs. NCBI nr
Match: gi|595824841|ref|XP_007205305.1| (hypothetical protein PRUPE_ppa006782mg [Prunus persica])

HSP 1 Score: 436.4 bits (1121), Expect = 5.5e-119
Identity = 236/386 (61.14%), Postives = 288/386 (74.61%), Query Frame = 1

Query: 28  GGAVDGASLRDSKRERVARERENSGE-ENMNEEVGQQDLTKRRVLRSRYLAVIHEISERR 87
           G   D   LR  KRERV     N+G  EN ++   +QD  +RR LRS YLAV + ISE+R
Sbjct: 15  GDVDDAEQLRAVKRERV---NANAGRVENESDLPSEQDSVQRRTLRSDYLAVKNLISEKR 74

Query: 88  EDLS-KDLDKFNVIINEVEKLHEQVQKPREQVADAEALQDIANSLVTSIRSQSNEGVTPS 147
           +DL   + ++F +IIN+VEKLHEQVQKPREQVADAEAL DI N+L TS++SQS+ G++PS
Sbjct: 75  DDLMCPESERFCLIINKVEKLHEQVQKPREQVADAEALLDITNTLWTSVKSQSSAGISPS 134

Query: 148 DFVSCLLREFADINGSINSEEQDLVSVNWKDIGLSVSSIFMNGYGCRTMLGPMSNQLKQR 207
           DFV+C+L  F  +N S+ S+E   VS+ WKDIGL+V+ IF   +GC TMLGPM+ +LKQR
Sbjct: 135 DFVTCVLNNFKQLNSSLASQEDAPVSIKWKDIGLAVAPIFKRAHGCCTMLGPMNTELKQR 194

Query: 208 KTNNVPRKRVRPTESSRPAEVEDNGSEPKTDTDKNMGIMFGILRRNKTVKLEHLILNRKS 267
           K   V  K  +PT + RP E++D   E KTDTDKNM  MF ILRRNK V+LE LILNRKS
Sbjct: 195 KAV-VRSKHAKPTTTDRPDEIDDTQGEEKTDTDKNMSTMFDILRRNKRVRLEGLILNRKS 254

Query: 268 FGQTVENLFALSFLVKDGRAEITIDKNGSHFVSPKNAPAHSAIMSHEVNYSHFVFRFDYK 327
           F QTVENLFALSFLVKDGRAEIT+D NGSH VSP+NAPA + + S EV Y HFVFRFD+K
Sbjct: 255 FAQTVENLFALSFLVKDGRAEITVDANGSHLVSPRNAPAANLVASREVVYDHFVFRFDFK 314

Query: 328 DWKPMVDMVPVGEELMPHRTFLNSVQVSQEEVAADNSESAGLPTTPIRKLSRNRGLVMQ- 387
           DWK M DM+PVGEELMPHR+  NS+  SQEE AA +S++A LPTTPIRKLSRNRG ++Q 
Sbjct: 315 DWKLMKDMLPVGEELMPHRSPPNSLPASQEEPAAYSSQTA-LPTTPIRKLSRNRGRLVQE 374

Query: 388 -SVVEDSPDNGSQSGATAILRGKRKL 410
            SVVE+SP+N   +G   + R KRKL
Sbjct: 375 ESVVEESPENDDAAGTNVVRRSKRKL 395

BLAST of Cucsa.143010 vs. NCBI nr
Match: gi|645277126|ref|XP_008243618.1| (PREDICTED: non-structural maintenance of chromosomes element 4 homolog A [Prunus mume])

HSP 1 Score: 433.3 bits (1113), Expect = 4.7e-118
Identity = 235/382 (61.52%), Postives = 285/382 (74.61%), Query Frame = 1

Query: 32  DGASLRDSKRERVARERENSGE-ENMNEEVGQQDLTKRRVLRSRYLAVIHEISERREDLS 91
           D   LR  KRERV     N+G  EN ++   +QD  +RR LRS YLAV + I E+R+DL 
Sbjct: 19  DAEQLRAVKRERV---HANAGRVENESDLPSEQDSVQRRTLRSDYLAVKNLICEKRDDLM 78

Query: 92  -KDLDKFNVIINEVEKLHEQVQKPREQVADAEALQDIANSLVTSIRSQSNEGVTPSDFVS 151
             + ++F +IIN+VEKLHEQVQKPREQVADAEAL DI N+L TS++SQS+ G++PSDFV+
Sbjct: 79  CPESERFCLIINKVEKLHEQVQKPREQVADAEALLDITNTLWTSVKSQSSAGISPSDFVT 138

Query: 152 CLLREFADINGSINSEEQDLVSVNWKDIGLSVSSIFMNGYGCRTMLGPMSNQLKQRKTNN 211
           C+L  F   N S+ S+E   VS+ WKDIGL+VS IF   +GC TMLGPM+ +LKQRK   
Sbjct: 139 CVLNNFKQSNSSLASQEDAPVSIKWKDIGLAVSPIFKRAHGCCTMLGPMNTELKQRKAV- 198

Query: 212 VPRKRVRPTESSRPAEVEDNGSEPKTDTDKNMGIMFGILRRNKTVKLEHLILNRKSFGQT 271
           V  K  +PT + RP E++D   E KTDTDKNM  MF ILRRNK V+LE LILNRKSF QT
Sbjct: 199 VRSKHAKPTTTDRPDEIDDTQGEEKTDTDKNMSTMFDILRRNKRVRLEGLILNRKSFAQT 258

Query: 272 VENLFALSFLVKDGRAEITIDKNGSHFVSPKNAPAHSAIMSHEVNYSHFVFRFDYKDWKP 331
           VENLFALSFLVKDGRAEIT+D NGSH VSP+NAPA + + S EV Y HFVFRFD+KDWK 
Sbjct: 259 VENLFALSFLVKDGRAEITVDANGSHLVSPRNAPAANLVASREVVYDHFVFRFDFKDWKL 318

Query: 332 MVDMVPVGEELMPHRTFLNSVQVSQEEVAADNSESAGLPTTPIRKLSRNRGLVMQ--SVV 391
           M DM+PVGEELMPHR+  NS+  SQEE AA +S++A LPTTPIRKLSRNRG ++Q  SVV
Sbjct: 319 MKDMLPVGEELMPHRSPPNSLPASQEEPAAYSSQTA-LPTTPIRKLSRNRGRLVQEESVV 378

Query: 392 EDSPDNGSQSGATAILRGKRKL 410
           E+SP+N   +G   + R KRKL
Sbjct: 379 EESPENDDAAGTNVVRRSKRKL 395

BLAST of Cucsa.143010 vs. NCBI nr
Match: gi|702273231|ref|XP_010043861.1| (PREDICTED: non-structural maintenance of chromosomes element 4 homolog A [Eucalyptus grandis])

HSP 1 Score: 426.4 bits (1095), Expect = 5.7e-116
Identity = 241/414 (58.21%), Postives = 293/414 (70.77%), Query Frame = 1

Query: 4   VKRE---PTVSTRSNRNRGGDGGGGGGGGAVDGASLRDSKRERVARERENSGEENMNEEV 63
           VKRE     VS+      G    G GGG A +   LR +KRER +R RE   E+  + + 
Sbjct: 68  VKRERLGKAVSSDGGAGVGSKRKGAGGGAAPE--QLRAAKRERSSRNRE---EKEPSSQA 127

Query: 64  GQQDLTKRRVLRSRYLAVIHEISERREDLSK-DLDKFNVIINEVEKLHEQVQKPREQVAD 123
            +QD ++RRVLRS YLAV + I++ R+DL + D DKF  II EVE L+E VQKPREQVAD
Sbjct: 128 TEQDDSERRVLRSHYLAVKNLINDERDDLLRADSDKFATIITEVENLYELVQKPREQVAD 187

Query: 124 AEALQDIANSLVTSIRSQSNEGVTPSDFVSCLLREFADINGSINSEEQDLVSVNWKDIGL 183
           AEAL  IAN+LV+S++S S++G+TPSDFV+CLL+EF    G +   E D V   WKD+G+
Sbjct: 188 AEALLGIANTLVSSVKSYSSDGITPSDFVNCLLKEFGQPMGGLGIGENDQVPFIWKDLGV 247

Query: 184 SVSSIFMNGYGCRTMLGPMSNQLKQRKTNNVPRKRVRPTESSRPAEVEDNGSEPKTDTDK 243
            VS IF N  GC TMLGPM+ QLKQRK   V R+  RPTE+SRP E++D G E KTDTDK
Sbjct: 248 RVSPIFRNFQGCCTMLGPMNTQLKQRKVT-VHRRHTRPTENSRPEEIDDAGVEKKTDTDK 307

Query: 244 NMGIMFGILRRNKTVKLEHLILNRKSFGQTVENLFALSFLVKDGRAEITIDKNGSHFVSP 303
           NM  MF ILRR K V L+ LILNR+SF QTVENLFALSFLVKDGRAE+T+D+ G HFVSP
Sbjct: 308 NMSTMFEILRRKKRVGLDSLILNRQSFAQTVENLFALSFLVKDGRAEVTVDEKGYHFVSP 367

Query: 304 KNAPAHSAIMSHEVNYSHFVFRFDYKDWKPMVDMVPVGEELMPHRTFLNSVQVSQEEVAA 363
           KNAP  S ++S EV Y HFVFRFDY+DWK M+  +P GEELMPHR        SQ ++ +
Sbjct: 368 KNAPVSSLVLSGEVAYGHFVFRFDYRDWKMMISTLPHGEELMPHREQQTCSAASQADLVS 427

Query: 364 DNSESAGLPTTPIRKLSRNRGLVMQ--SVVEDSP--DNGSQSGATAILRGKRKL 410
            NS+S  LPTTPIRKL+RNRGLV+Q  SVVEDSP  D+ + S A AI + KRKL
Sbjct: 428 CNSQSV-LPTTPIRKLTRNRGLVIQEESVVEDSPVVDDDTSSRANAIRKCKRKL 474

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
NSE4A_ARATH7.5e-10050.74Non-structural maintenance of chromosomes element 4 homolog A OS=Arabidopsis tha... [more]
NSE4B_ARATH9.8e-9251.93Non-structural maintenance of chromosomes element 4 homolog B OS=Arabidopsis tha... [more]
NSE4A_BOVIN2.6e-1523.10Non-structural maintenance of chromosomes element 4 homolog A OS=Bos taurus GN=N... [more]
NSE4A_HUMAN1.7e-1422.57Non-structural maintenance of chromosomes element 4 homolog A OS=Homo sapiens GN... [more]
NSE4_SCHPO2.0e-1222.97Non-structural maintenance of chromosome element 4 OS=Schizosaccharomyces pombe ... [more]
Match NameE-valueIdentityDescription
A0A0A0LZJ0_CUCSA4.3e-235100.00Uncharacterized protein OS=Cucumis sativus GN=Csa_1G605680 PE=4 SV=1[more]
M5VYK3_PRUPE3.9e-11961.14Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa006782mg PE=4 SV=1[more]
A0A059D627_EUCGR4.0e-11658.21Uncharacterized protein OS=Eucalyptus grandis GN=EUGRSUZ_B02593 PE=4 SV=1[more]
F6HLJ8_VITVI1.7e-11460.53Putative uncharacterized protein OS=Vitis vinifera GN=VIT_08s0007g06380 PE=4 SV=... [more]
A0A061GSH5_THECC1.7e-11460.05Nse4, component of Smc5/6 DNA repair complex, putative isoform 1 OS=Theobroma ca... [more]
Match NameE-valueIdentityDescription
AT1G51130.14.2e-10150.74 Nse4, component of Smc5/6 DNA repair complex[more]
AT3G20760.15.5e-9351.93 Nse4, component of Smc5/6 DNA repair complex[more]
Match NameE-valueIdentityDescription
gi|449462613|ref|XP_004149035.1|6.1e-235100.00PREDICTED: non-structural maintenance of chromosomes element 4 homolog A-like [C... [more]
gi|659100485|ref|XP_008451119.1|1.6e-21995.39PREDICTED: non-structural maintenance of chromosomes element 4 homolog A [Cucumi... [more]
gi|595824841|ref|XP_007205305.1|5.5e-11961.14hypothetical protein PRUPE_ppa006782mg [Prunus persica][more]
gi|645277126|ref|XP_008243618.1|4.7e-11861.52PREDICTED: non-structural maintenance of chromosomes element 4 homolog A [Prunus... [more]
gi|702273231|ref|XP_010043861.1|5.7e-11658.21PREDICTED: non-structural maintenance of chromosomes element 4 homolog A [Eucaly... [more]
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR014854Nse4_C
IPR027786Nse4/EID
Vocabulary: Cellular Component
TermDefinition
GO:0005634nucleus
GO:0030915Smc5-Smc6 complex
Vocabulary: Biological Process
TermDefinition
GO:0006281DNA repair
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006281 DNA repair
cellular_component GO:0005634 nucleus
cellular_component GO:0030915 Smc5-Smc6 complex
molecular_function GO:0003674 molecular_function

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cucsa.143010.1Cucsa.143010.1mRNA


Analysis Name: InterPro Annotations of cucumber (Gy14)
Date Performed: 2017-01-17
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR014854Non-structural maintenance of chromosome element 4, C-terminalPFAMPF08743Nse4_Ccoord: 254..343
score: 2.7
IPR027786Nse4/EID familyPANTHERPTHR16140UNCHARACTERIZEDcoord: 55..402
score: 9.3E
NoneNo IPR availablePANTHERPTHR16140:SF5SUBFAMILY NOT NAMEDcoord: 55..402
score: 9.3E

The following gene(s) are paralogous to this gene:

None