Cucsa.100990 (gene) Cucumber (Gy14) v1 | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Cucsa.100990
Type	gene
Organism	Cucumis sativus (Cucumber (Gy14) v1)
Description	Pectinesterase inhibitor
Location	scaffold00927 : 271935 .. 273132 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGTATGAAGAATTTCTCTATTTCTCTCATTTtCTTtGCAATCCCATTAAtCTTTTtCCACAAAAaTAATGGAGTTTCATTAGCATCAGCTGACCAAACCCTAATCCAAAAAaCATGCACAAACACTCTTTACTACAAACTTTGCATGTCATCCCTTAAATCCGACCCCGCCAGCCTCACCGCCGACACCAAAGGCCTTGCCGTCATCATGGCTTCCATCGGTGCTGCTAACGCCACCGCCACCGCCAGCTACCTCTCGTCTCAGCTCCCTACATCCTCCTCCGGTGCCGGTGCCAACAACAACAAAACAAAATTGCTGAGACAATGCTCCGAGAAATACGCATTTGCAGCTGAAGCACTTAGAGAATCTTTGAAAGATTTGGGTGATGAGACTTTTGACTATGCTTATATGCATGTTTCTGCGGCGGCGGATTATGCCAATGTTTGCCGTGATGCCTTTAAAGGGTTCCCAGCGGTGAGTTATCCGACGAAGCTTGGCCGGAGAGAAGAAGGGTTGAAGCGTATTTGCAGAGTGGTTTTGGGGATTTTGGATCTTCTTGGCTGGTGATTTTCATCAATCATTATGTTCTCTCTCTAATCATATTTTTGTTTTTCTTTTTTTTttACTTTTACTTTTTGCTTTTACTTTTTTTtGGGTAATAAACTTGTTTGTCTTTTTAGGTGAAACGTTGATGATGCTTTTCTTTAAGGGATTTAATGACTCCTTTATTTGTTAAAATAATTTATTTCTTCTACAGTAAGATCTCATGTGTTTGAATTAGGTTTAGTAATGACACTTTTGATGTTGTAAACTTATAGAGGATTTTATATTTTTGTCATTCTTTTTTtAAGTTTTGAGTTATGATGGATAATATATAATAGGTCGAATCAAAACCTCTTATCTATCTAAGTTGTAATATTTTTAATTTTGTATGTTCGAATTTTTTGATATCTATCCGTTTTCTTATAATATTTTATTCATATATCGAGTAAAGTTTAGTATATATGACCTTACACGTTATCGGAATTATTAATAAAACCATCACGTAATAATATTAATTTTTtAAAAGAATTTGATAAATTTTAGTGGTTAGTGAATAAAATGATAGTTGTTGTGGTTCTCTTCCCTAATCAAAGTTGAGATGGGGTTTTTGCTTTTGCTTTTAAAGTATATGAACCAAATATATCCATAGACA

mRNA sequence

ATGGGTATGAAGAATTTCTCTATTTCTCTCATTTTCTTTGCAATCCCATTAATCTTTTTCCACAAAAATAATGGAGTTTCATTAGCATCAGCTGACCAAACCCTAATCCAAAAAACATGCACAAACACTCTTTACTACAAACTTTGCATGTCATCCCTTAAATCCGACCCCGCCAGCCTCACCGCCGACACCAAAGGCCTTGCCGTCATCATGGCTTCCATCGGTGCTGCTAACGCCACCGCCACCGCCAGCTACCTCTCGTCTCAGCTCCCTACATCCTCCTCCGGTGCCGGTGCCAACAACAACAAAACAAAATTGCTGAGACAATGCTCCGAGAAATACGCATTTGCAGCTGAAGCACTTAGAGAATCTTTGAAAGATTTGGGTGATGAGACTTTTGACTATGCTTATATGCATGTTTCTGCGGCGGCGGATTATGCCAATGTTTGCCGTGATGCCTTTAAAGGGTTCCCAGCGGTGAGTTATCCGACGAAGCTTGGCCGGAGAGAAGAAGGGTTGAAGCGTATTTGCAGAGTGGTTTTGGGGATTTTGGATCTTCTTGGCTGGTGATTTTCATCAATCATTATGTTCTCTCTCTAATCATAtttttgtttttcttttttttttacttttactttttgcttttacttttttttGGGTAATAAACTTGTTTGTCTTTTTAGGTGAAACGTTGATGATGCTTTTCTTTAAGGGATTTAATGACTCCTTTATTTGTTAAAATAATTTATTTCTTCTACAGTAAGATCTCATGTGTTTGAATTAGGTTTAGTAATGACACTTTTGATGTTGTAAACTTATAGAGGATTTTATATTTTTGTCATTCTTTTTTTAAGTTTTGAGTTATGATGGATAATATATAATAGGTCGAATCAAAACCTCTTATCTATCTAAGTTGTAATATTTTTAATTTTGTATGTTCGAATTTTTTGATATCTATCCGTTTTCTTATAATATTTTATTCATATATCGAGTAAAGTTTAGTATATATGACCTTACACGTTATCGGAATTATTAATAAAACCATCACGtaataatattaattttttaaaagaatttgataaattttaGTGGTTAGTGAATAAAATGATAGTTGTTGTGGTTCTCTTCCCTAATCAAAGTTGAGATGGGGTTTTTGCTTTTGCTTTTAAAGTATATGAACCAAATATATCCATAGACA

Coding sequence (CDS)

ATGGGTATGAAGAATTTCTCTATTTCTCTCATTTtCTTtGCAATCCCATTAAtCTTTTtCCACAAAAaTAATGGAGTTTCATTAGCATCAGCTGACCAAACCCTAATCCAAAAAaCATGCACAAACACTCTTTACTACAAACTTTGCATGTCATCCCTTAAATCCGACCCCGCCAGCCTCACCGCCGACACCAAAGGCCTTGCCGTCATCATGGCTTCCATCGGTGCTGCTAACGCCACCGCCACCGCCAGCTACCTCTCGTCTCAGCTCCCTACATCCTCCTCCGGTGCCGGTGCCAACAACAACAAAACAAAATTGCTGAGACAATGCTCCGAGAAATACGCATTTGCAGCTGAAGCACTTAGAGAATCTTTGAAAGATTTGGGTGATGAGACTTTTGACTATGCTTATATGCATGTTTCTGCGGCGGCGGATTATGCCAATGTTTGCCGTGATGCCTTTAAAGGGTTCCCAGCGGTGAGTTATCCGACGAAGCTTGGCCGGAGAGAAGAAGGGTTGAAGCGTATTTGCAGAGTGGTTTTGGGGATTTTGGATCTTCTTGGCTGGTGA

Protein sequence

MGMKNFSISLIFFAIPLIFFHKNNGVSLASADQTLIQKTCTNTLYYKLCMSSLKSDPASLTADTKGLAVIMASIGAANATATASYLSSQLPTSSSGAGANNNKTKLLRQCSEKYAFAAEALRESLKDLGDETFDYAYMHVSAAADYANVCRDAFKGFPAVSYPTKLGRREEGLKRICRVVLGILDLLGW*

Homology

[Close]

BLAST of Cucsa.100990 vs. Swiss-Prot
Match: CVIF2_ARATH (Cell wall / vacuolar inhibitor of fructosidase 2 OS=Arabidopsis thaliana GN=C/VIF2 PE=1 SV=1)

HSP 1 Score: 170.6 bits (431), Expect = 1.6e-41
Identity = 89/179 (49.72%), Postives = 118/179 (65.92%), Query Frame = 1


Query: 9   SLIFFAIPLIFFHKNNGVSLASADQTLIQKTCTNTLYYKLCMSSLKSDPASLTADTKGLA 68
           SLIF  +  + F  +  +S  S   T+I+ TC  T YYK C+S+LKSDP S TADTKGLA
Sbjct: 4   SLIFLLLVTLTFSASTLISAKSNTTTIIESTCKTTNYYKFCVSALKSDPRSPTADTKGLA 63

Query: 69  VIMASIGAANATATASYLSSQLPTSSSGAGANNNKTKLLRQCSEKYAFAAEALRESLKDL 128
            IM  +G  NAT+TA+Y++  L    S    +    K+L+ CSEKYA AA++LR +++DL
Sbjct: 64  SIMVGVGMTNATSTANYIAGNL----SATVKDTVLKKVLQDCSEKYALAADSLRLTIQDL 123

Query: 129 GDETFDYAYMHVSAAADYANVCRDAFKGFPAVSYPTKLGRREEGLKRICRVVLGILDLL 188
            DE +DYA MHV AA DY NVCR+ F+    ++YP ++ RRE  L+RIC VV GILD L
Sbjct: 124 DDEAYDYASMHVLAAQDYPNVCRNIFRRVKGLAYPVEIRRREASLRRICGVVSGILDRL 178

[Close]

BLAST of Cucsa.100990 vs. Swiss-Prot
Match: PLA1_PLAAC (Putative invertase inhibitor OS=Platanus acerifolia PE=1 SV=1)

HSP 1 Score: 65.1 bits (157), Expect = 9.6e-10
Identity = 51/190 (26.84%), Postives = 89/190 (46.84%), Query Frame = 1


Query: 5   NFSISLIFFAIPLIFFHKNNGVSLASADQTLIQKTCTNT------LYYKLCMSSLKSDPA 64
           +FS+ + FF + L+        ++ SAD  ++Q TC         + Y  C+ SL +DP 
Sbjct: 4   SFSLCIFFFNLLLLL------QAVISAD--IVQGTCKKVAQRSPNVNYDFCVKSLGADPK 63

Query: 65  SLTADTKGLAVIMASIGAANATATASYLSSQLPTSSSGAGANNNKTKLLRQCSEKYAFAA 124
           S TAD +GL VI A++   + +   +++   L +    A       K L  C   YA A 
Sbjct: 64  SHTADLQGLGVISANLAIQHGSKIQTFIGRILKSKVDPA-----LKKYLNDCVGLYADAK 123

Query: 125 EALRESLKDLGDETFDYAYMHVSAAADYANVCRDAFKGFPAVSYPTKLGRREEGLKRICR 184
            +++E++ D   + +  A + +SAA D +  C D FK    +  P  + +  +   ++  
Sbjct: 124 SSVQEAIADFKSKDYASANVKMSAALDDSVTCEDGFKEKKGIVSP--VTKENKDYVQLTA 178

Query: 185 VVLGILDLLG 189
           + L I  LLG
Sbjct: 184 ISLAITKLLG 178

[Close]

BLAST of Cucsa.100990 vs. TrEMBL
Match: A0A0A0KJ03_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_6G483430 PE=4 SV=1)

HSP 1 Score: 374.4 bits (960), Expect = 8.2e-101
Identity = 189/189 (100.00%), Postives = 189/189 (100.00%), Query Frame = 1


Query: 1   MGMKNFSISLIFFAIPLIFFHKNNGVSLASADQTLIQKTCTNTLYYKLCMSSLKSDPASL 60
           MGMKNFSISLIFFAIPLIFFHKNNGVSLASADQTLIQKTCTNTLYYKLCMSSLKSDPASL
Sbjct: 1   MGMKNFSISLIFFAIPLIFFHKNNGVSLASADQTLIQKTCTNTLYYKLCMSSLKSDPASL 60

Query: 61  TADTKGLAVIMASIGAANATATASYLSSQLPTSSSGAGANNNKTKLLRQCSEKYAFAAEA 120
           TADTKGLAVIMASIGAANATATASYLSSQLPTSSSGAGANNNKTKLLRQCSEKYAFAAEA
Sbjct: 61  TADTKGLAVIMASIGAANATATASYLSSQLPTSSSGAGANNNKTKLLRQCSEKYAFAAEA 120

Query: 121 LRESLKDLGDETFDYAYMHVSAAADYANVCRDAFKGFPAVSYPTKLGRREEGLKRICRVV 180
           LRESLKDLGDETFDYAYMHVSAAADYANVCRDAFKGFPAVSYPTKLGRREEGLKRICRVV
Sbjct: 121 LRESLKDLGDETFDYAYMHVSAAADYANVCRDAFKGFPAVSYPTKLGRREEGLKRICRVV 180

Query: 181 LGILDLLGW 190
           LGILDLLGW
Sbjct: 181 LGILDLLGW 189

[Close]

BLAST of Cucsa.100990 vs. TrEMBL
Match: M5W386_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa021753mg PE=4 SV=1)

HSP 1 Score: 208.8 bits (530), Expect = 6.0e-51
Identity = 105/183 (57.38%), Postives = 133/183 (72.68%), Query Frame = 1


Query: 7   SISLIFFAIPLIFFHKNNGVSLASADQTLIQKTCTNTLYYKLCMSSLKSDPASLTADTKG 66
           SI    F + L F H+ +     +AD  LIQKTC NT YY LC+S+LKSDP S TADTKG
Sbjct: 3   SIIFSLFLLSLAFPHQTHNF-FVTADSNLIQKTCKNTKYYGLCVSTLKSDPTSKTADTKG 62

Query: 67  LAVIMASIGAANATATASYLSSQLPTSSSGAGANNNKTKLLRQCSEKYAFAAEALRESLK 126
           LAVIM  IG ANATAT+SYLSSQL +S +    + N  K+L++C++KY FA +AL+ S+K
Sbjct: 63  LAVIMVGIGMANATATSSYLSSQLVSSRNN---DTNMKKVLKECADKYGFAGDALQASVK 122

Query: 127 DLGDETFDYAYMHVSAAADYANVCRDAFKGFPAVSYPTKLGRREEGLKRICRVVLGILDL 186
           DL  E++DYA MH++AAADY N C +AFK +PA++YP +L RREEGLK IC V LGI+D 
Sbjct: 123 DLASESYDYASMHINAAADYPNACHNAFKRYPALAYPPELARREEGLKHICDVALGIIDN 181

Query: 187 LGW 190
            GW
Sbjct: 183 FGW 181

[Close]

BLAST of Cucsa.100990 vs. TrEMBL
Match: W9SL32_9ROSA (Pectinesterase inhibitor OS=Morus notabilis GN=L484_013831 PE=4 SV=1)

HSP 1 Score: 200.7 bits (509), Expect = 1.6e-48
Identity = 100/183 (54.64%), Postives = 135/183 (73.77%), Query Frame = 1


Query: 8   ISLIFFAIPLIFFHKNNGVSLASADQTLIQKTCTNTLYYKLCMSSLKSDPASLTA-DTKG 67
           +SL+  + P    H ++   LA+AD T IQ+TC  T +Y LC+SSLKSDP SL A D KG
Sbjct: 14  LSLLLLSFP----HGHHRRLLAAADATFIQQTCKTTKHYDLCVSSLKSDPTSLQASDAKG 73

Query: 68  LAVIMASIGAANATATASYLSSQLPTSSSGAGANNNKTKLLRQCSEKYAFAAEALRESLK 127
           LA IM +IG AN+TAT++Y+SSQLP++++   A   K  + ++C++KY +AAEAL+ S +
Sbjct: 74  LANIMIAIGVANSTATSAYISSQLPSTTTTTAAAAKKKAVFKECADKYGYAAEALQASAQ 133

Query: 128 DLGDETFDYAYMHVSAAADYANVCRDAFKGFPAVSYPTKLGRREEGLKRICRVVLGILDL 187
           DL  E+FDY YMHV+AAADY N C +AF+ +P + YPT+L RRE+GLKRIC VVLGI+D 
Sbjct: 134 DLAAESFDYTYMHVTAAADYPNSCHNAFRRYPGLPYPTELARREDGLKRICDVVLGIIDN 192

Query: 188 LGW 190
           LGW
Sbjct: 194 LGW 192

[Close]

BLAST of Cucsa.100990 vs. TrEMBL
Match: A0A0R0JHD5_SOYBN (Uncharacterized protein OS=Glycine max GN=GLYMA_06G152400 PE=4 SV=1)

HSP 1 Score: 194.1 bits (492), Expect = 1.5e-46
Identity = 95/181 (52.49%), Postives = 128/181 (70.72%), Query Frame = 1


Query: 9   SLIFFAIPLIFFHKNNGVSLASADQTLIQKTCTNTLYYKLCMSSLKSDPASLTADTKGLA 68
           S IFF   L   H +   S+   D +LI++TC NT YY LC SSLKSDP+S  AD KGLA
Sbjct: 4   SKIFFLFLLFLAHLHQHASV-EGDSSLIKRTCKNTKYYNLCFSSLKSDPSSPNADPKGLA 63

Query: 69  VIMASIGAANATATASYLSSQLPTSSSGAGANNNKTKLLRQCSEKYAFAAEALRESLKDL 128
           VIM  IG  NAT+T+SYLSS+LPT S+    N    ++L++C++KY++A +AL++S++DL
Sbjct: 64  VIMIGIGMTNATSTSSYLSSKLPTPSN----NTTWKRVLKECADKYSYAGDALQDSVQDL 123

Query: 129 GDETFDYAYMHVSAAADYANVCRDAFKGFPAVSYPTKLGRREEGLKRICRVVLGILDLLG 188
            +E +DYAYMH++AA DY N C +AFK +P + YP  L RRE+GLK IC V +GI+D L 
Sbjct: 124 ANEAYDYAYMHITAAKDYPNACHNAFKRYPGLVYPRDLARREDGLKHICDVAMGIIDNLD 179

Query: 189 W 190
           W
Sbjct: 184 W 179

[Close]

BLAST of Cucsa.100990 vs. TrEMBL
Match: A0A0S3RF88_PHAAN (Uncharacterized protein OS=Vigna angularis var. angularis GN=Vigan.02G207200 PE=4 SV=1)

HSP 1 Score: 190.7 bits (483), Expect = 1.7e-45
Identity = 93/181 (51.38%), Postives = 130/181 (71.82%), Query Frame = 1


Query: 9   SLIFFAIPLIFFHKNNGVSLASADQTLIQKTCTNTLYYKLCMSSLKSDPASLTADTKGLA 68
           S IFF + L+F    +  +  + D TLI++TC NT YY LC SSLKSDP+S  AD KGLA
Sbjct: 3   SKIFFLLFLLFLLYPHQHACVNGDATLIKRTCKNTKYYNLCFSSLKSDPSSPNADPKGLA 62

Query: 69  VIMASIGAANATATASYLSSQLPTSSSGAGANNNKTKLLRQCSEKYAFAAEALRESLKDL 128
           +IM  IG +NAT+T+SYLSS+L + S+    N    +++++C++KY +A +AL+ S++DL
Sbjct: 63  MIMIGIGMSNATSTSSYLSSKLLSPSN----NTTLKRVIKECADKYTYAGDALQASVEDL 122

Query: 129 GDETFDYAYMHVSAAADYANVCRDAFKGFPAVSYPTKLGRREEGLKRICRVVLGILDLLG 188
            +E +DYAYMH++AA DY N C +AFK +PA+SYP  L RRE+GLK IC V +GI+D L 
Sbjct: 123 LNEAYDYAYMHITAAKDYPNACHNAFKRYPALSYPLDLARREDGLKHICDVAMGIIDNLD 179

Query: 189 W 190
           W
Sbjct: 183 W 179

[Close]

BLAST of Cucsa.100990 vs. TAIR10
Match: AT5G64620.1 (AT5G64620.1 cell wall / vacuolar inhibitor of fructosidase 2)

HSP 1 Score: 170.6 bits (431), Expect = 9.1e-43
Identity = 89/179 (49.72%), Postives = 118/179 (65.92%), Query Frame = 1


Query: 9   SLIFFAIPLIFFHKNNGVSLASADQTLIQKTCTNTLYYKLCMSSLKSDPASLTADTKGLA 68
           SLIF  +  + F  +  +S  S   T+I+ TC  T YYK C+S+LKSDP S TADTKGLA
Sbjct: 4   SLIFLLLVTLTFSASTLISAKSNTTTIIESTCKTTNYYKFCVSALKSDPRSPTADTKGLA 63

Query: 69  VIMASIGAANATATASYLSSQLPTSSSGAGANNNKTKLLRQCSEKYAFAAEALRESLKDL 128
            IM  +G  NAT+TA+Y++  L    S    +    K+L+ CSEKYA AA++LR +++DL
Sbjct: 64  SIMVGVGMTNATSTANYIAGNL----SATVKDTVLKKVLQDCSEKYALAADSLRLTIQDL 123

Query: 129 GDETFDYAYMHVSAAADYANVCRDAFKGFPAVSYPTKLGRREEGLKRICRVVLGILDLL 188
            DE +DYA MHV AA DY NVCR+ F+    ++YP ++ RRE  L+RIC VV GILD L
Sbjct: 124 DDEAYDYASMHVLAAQDYPNVCRNIFRRVKGLAYPVEIRRREASLRRICGVVSGILDRL 178

[Close]

BLAST of Cucsa.100990 vs. TAIR10
Match: AT5G46940.1 (AT5G46940.1 Plant invertase/pectin methylesterase inhibitor superfamily protein)

HSP 1 Score: 55.8 bits (133), Expect = 3.3e-08
Identity = 50/183 (27.32%), Postives = 81/183 (44.26%), Query Frame = 1


Query: 12  FFAIPLIFFHKNNGVSLASADQTLIQKTCTNT------LYYKLCMSSLKSDPASLTA-DT 71
           F    + FF  +NG++     QTLI+ +C           Y LC++SL+++P + TA D 
Sbjct: 3   FLLYLVTFFVLSNGLANG---QTLIRNSCKKATATSPKFKYNLCVTSLETNPQAKTAKDL 62

Query: 72  KGLAVIMASIGAANATATASYLSSQLPTSSSGAGANNNKTKLLRQCSEKYAFAAEALRES 131
            GL  +MAS    NA   A+ L   +     G   N      LR C + Y  A  +L E+
Sbjct: 63  AGL--VMAS--TKNAVTKATTLKGTVDKIIKGKKVNKMTAMPLRDCLQLYTDAIGSLNEA 122

Query: 132 LKDLGDETFDYAYMHVSAAADYANVCRDAFKGFPAVSYPTKLGRREEGLKRICRVVLGIL 188
           L  +    +      +SAA D  + C   FK   A   P+ + +  + L ++  + L   
Sbjct: 123 LAGVKSRNYPTVKTVLSAAMDTPSTCETGFKERKA---PSPVTKENDNLYQMILIPLAFT 175

[Close]

BLAST of Cucsa.100990 vs. TAIR10
Match: AT1G55770.1 (AT1G55770.1 Plant invertase/pectin methylesterase inhibitor superfamily protein)

HSP 1 Score: 51.2 bits (121), Expect = 8.1e-07
Identity = 46/183 (25.14%), Postives = 76/183 (41.53%), Query Frame = 1


Query: 7   SISLIFFAIPLIFFHKNNGVSLASADQTLIQKTCTNTLYYKLCMSSLKSDPAS--LTADT 66
           S  ++F  I LI F   N      A+  +I   C ++    LC+SS+ S P S      +
Sbjct: 4   SAGVLFLCIVLISFVTGN------ANSGMISDLCKHSDDPNLCLSSITSRPESGEFAGTS 63

Query: 67  KGLAVIMASIGAANATATASYLSSQLPTSSSGAGANNNKTKLLRQCSEKYAFAAEALRES 126
             + +I  S  +ANA+AT+SY+  +L          +     L  C + Y  A E L +S
Sbjct: 64  NQIEIIAISAASANASATSSYIKQKLSNEDLEPAIEDT----LEDCQKDYQDAVEQLDDS 123

Query: 127 LKDLGDETFDYAYMHVSAAADYANVCRDAFKGFPAVSYPTKLGRREEGLKRICRVVLGIL 186
           +  +  +      + +SAA      C  A           +L +R E   ++C+  L I 
Sbjct: 124 ISAMLADAHTDVDVWLSAAISAIESCGSALGS--RAGNDAELSQRNEVFLKLCKNALMIN 174

Query: 187 DLL 188
            +L
Sbjct: 184 KML 174

[Close]

BLAST of Cucsa.100990 vs. TAIR10
Match: AT1G47960.1 (AT1G47960.1 cell wall / vacuolar inhibitor of fructosidase 1)

HSP 1 Score: 48.5 bits (114), Expect = 5.2e-06
Identity = 39/144 (27.08%), Postives = 67/144 (46.53%), Query Frame = 1


Query: 26  VSLASADQTLIQKTCTNTLYYKLCMSSLKSDPASLTADTKGLAVIMASIGAANATATASY 85
           V +  ++ ++I+ TC  T  + LC+S L SDP   +ADT GLA+I+       AT T + 
Sbjct: 16  VMVMVSEGSIIEPTCKETPDFNLCVSLLNSDPRGSSADTSGLALILIDKIKGLATKTLNE 75

Query: 86  LSSQLPTSSSGAGANNNKTKLLR---QCSEKYAFAAEA-LRESLKDLGDETFDYAYMHVS 145
           ++          G    + +L R   +CS +Y     A + E+++ +      +    V 
Sbjct: 76  IN----------GLYKKRPELKRALDECSRRYKTILNADVPEAIEAISKGVPKFGEDGVI 135

Query: 146 AAADYANVCRDAFKGFPAVSYPTK 166
            A   A+VC+  F G   ++  TK
Sbjct: 136 DAGVEASVCQGGFNGSSPLTSLTK 149

[Close]

BLAST of Cucsa.100990 vs. NCBI nr
Match: gi|449461841|ref|XP_004148650.1| (PREDICTED: cell wall / vacuolar inhibitor of fructosidase 2 [Cucumis sativus])

HSP 1 Score: 374.4 bits (960), Expect = 1.2e-100
Identity = 189/189 (100.00%), Postives = 189/189 (100.00%), Query Frame = 1


Query: 1   MGMKNFSISLIFFAIPLIFFHKNNGVSLASADQTLIQKTCTNTLYYKLCMSSLKSDPASL 60
           MGMKNFSISLIFFAIPLIFFHKNNGVSLASADQTLIQKTCTNTLYYKLCMSSLKSDPASL
Sbjct: 1   MGMKNFSISLIFFAIPLIFFHKNNGVSLASADQTLIQKTCTNTLYYKLCMSSLKSDPASL 60

Query: 61  TADTKGLAVIMASIGAANATATASYLSSQLPTSSSGAGANNNKTKLLRQCSEKYAFAAEA 120
           TADTKGLAVIMASIGAANATATASYLSSQLPTSSSGAGANNNKTKLLRQCSEKYAFAAEA
Sbjct: 61  TADTKGLAVIMASIGAANATATASYLSSQLPTSSSGAGANNNKTKLLRQCSEKYAFAAEA 120

Query: 121 LRESLKDLGDETFDYAYMHVSAAADYANVCRDAFKGFPAVSYPTKLGRREEGLKRICRVV 180
           LRESLKDLGDETFDYAYMHVSAAADYANVCRDAFKGFPAVSYPTKLGRREEGLKRICRVV
Sbjct: 121 LRESLKDLGDETFDYAYMHVSAAADYANVCRDAFKGFPAVSYPTKLGRREEGLKRICRVV 180

Query: 181 LGILDLLGW 190
           LGILDLLGW
Sbjct: 181 LGILDLLGW 189

[Close]

BLAST of Cucsa.100990 vs. NCBI nr
Match: gi|659080910|ref|XP_008441045.1| (PREDICTED: cell wall / vacuolar inhibitor of fructosidase 2 [Cucumis melo])

HSP 1 Score: 349.0 bits (894), Expect = 5.3e-93
Identity = 179/190 (94.21%), Postives = 184/190 (96.84%), Query Frame = 1


Query: 1   MGMKNFSISLIFFAIPLIFFHKNNGVSLASADQTLIQKTCTNTLYYKLCMSSLKSDPASL 60
           MGMKNFSISLIFFAIPLIFFHKN GVSLASADQTLIQKTCTNTLYYKLCMSSLKSDP+SL
Sbjct: 1   MGMKNFSISLIFFAIPLIFFHKN-GVSLASADQTLIQKTCTNTLYYKLCMSSLKSDPSSL 60

Query: 61  TADTKGLAVIMASIGAANATATASYLSSQLPTSSSGA-GANNNKTKLLRQCSEKYAFAAE 120
           TADTKGLA+IMASIGAANATAT++YLSSQLPTSSS A  ANNNKTKLLRQCSEKYAFAAE
Sbjct: 61  TADTKGLAIIMASIGAANATATSTYLSSQLPTSSSAATNANNNKTKLLRQCSEKYAFAAE 120

Query: 121 ALRESLKDLGDETFDYAYMHVSAAADYANVCRDAFKGFPAVSYPTKLGRREEGLKRICRV 180
           ALRESLKDL DET+DYAYMHVSAAADYANVCRDAFKGFPAVSYP KLGRREEGLKRICRV
Sbjct: 121 ALRESLKDLADETYDYAYMHVSAAADYANVCRDAFKGFPAVSYPAKLGRREEGLKRICRV 180

Query: 181 VLGILDLLGW 190
           VLGILDLLGW
Sbjct: 181 VLGILDLLGW 189

[Close]

BLAST of Cucsa.100990 vs. NCBI nr
Match: gi|595833188|ref|XP_007206622.1| (hypothetical protein PRUPE_ppa021753mg [Prunus persica])

HSP 1 Score: 208.8 bits (530), Expect = 8.6e-51
Identity = 105/183 (57.38%), Postives = 133/183 (72.68%), Query Frame = 1


Query: 7   SISLIFFAIPLIFFHKNNGVSLASADQTLIQKTCTNTLYYKLCMSSLKSDPASLTADTKG 66
           SI    F + L F H+ +     +AD  LIQKTC NT YY LC+S+LKSDP S TADTKG
Sbjct: 3   SIIFSLFLLSLAFPHQTHNF-FVTADSNLIQKTCKNTKYYGLCVSTLKSDPTSKTADTKG 62

Query: 67  LAVIMASIGAANATATASYLSSQLPTSSSGAGANNNKTKLLRQCSEKYAFAAEALRESLK 126
           LAVIM  IG ANATAT+SYLSSQL +S +    + N  K+L++C++KY FA +AL+ S+K
Sbjct: 63  LAVIMVGIGMANATATSSYLSSQLVSSRNN---DTNMKKVLKECADKYGFAGDALQASVK 122

Query: 127 DLGDETFDYAYMHVSAAADYANVCRDAFKGFPAVSYPTKLGRREEGLKRICRVVLGILDL 186
           DL  E++DYA MH++AAADY N C +AFK +PA++YP +L RREEGLK IC V LGI+D 
Sbjct: 123 DLASESYDYASMHINAAADYPNACHNAFKRYPALAYPPELARREEGLKHICDVALGIIDN 181

Query: 187 LGW 190
            GW
Sbjct: 183 FGW 181

[Close]

BLAST of Cucsa.100990 vs. NCBI nr
Match: gi|658047079|ref|XP_008359213.1| (PREDICTED: cell wall / vacuolar inhibitor of fructosidase 2-like [Malus domestica])

HSP 1 Score: 203.8 bits (517), Expect = 2.8e-49
Identity = 103/183 (56.28%), Postives = 132/183 (72.13%), Query Frame = 1


Query: 6   FSISLIFFAIPLIFFHKNNGVSLASADQTLIQKTCTNTLYYKLCMSSLKSDPASLTADTK 65
           +S   + F +   F H+N    L  AD TLIQ TC NT YY LC+SSLKS P S  ADTK
Sbjct: 43  YSTIFLLFLLTTTFPHQNPNF-LVHADSTLIQNTCKNTKYYGLCVSSLKSVPTSKNADTK 102

Query: 66  GLAVIMASIGAANATATASYLSSQLPTSSSGAGANNNKTKLLRQCSEKYAFAAEALRESL 125
           GLAVIM  IG ANATAT+SYLSSQL +SS+    + N  K+L++C++KY +A +AL+ S+
Sbjct: 103 GLAVIMVGIGMANATATSSYLSSQLISSST---TDTNLKKVLKECADKYGYAGDALQASV 162

Query: 126 KDLGDETFDYAYMHVSAAADYANVCRDAFKGFPAVSYPTKLGRREEGLKRICRVVLGILD 185
           +DLG E++DYAYMH++AAADY N C +AFK +P ++YP +L RREEGLK IC V LGI+D
Sbjct: 163 QDLGSESYDYAYMHITAAADYPNACHNAFKRYPGLAYPPELARREEGLKHICDVALGIID 221

Query: 186 LLG 189
             G
Sbjct: 223 NFG 221

[Close]

BLAST of Cucsa.100990 vs. NCBI nr
Match: gi|645219391|ref|XP_008235482.1| (PREDICTED: cell wall / vacuolar inhibitor of fructosidase 2-like [Prunus mume])

HSP 1 Score: 203.4 bits (516), Expect = 3.6e-49
Identity = 104/189 (55.03%), Postives = 133/189 (70.37%), Query Frame = 1


Query: 1   MGMKNFSISLIFFAIPLIFFHKNNGVSLASADQTLIQKTCTNTLYYKLCMSSLKSDPASL 60
           MG   FS+ L+  A P             +AD  LIQKTC NT YY LC+S+LKSDP S 
Sbjct: 1   MGSIIFSLFLLSLAFP-----PQTHNFFVTADSNLIQKTCKNTKYYGLCVSTLKSDPTSK 60

Query: 61  TADTKGLAVIMASIGAANATATASYLSSQLPTSSSGAGANNNKTKLLRQCSEKYAFAAEA 120
           TADTKGLAVIM  IG ANA+AT+SYLSSQL +S +    + N  K+L++C++KY FA +A
Sbjct: 61  TADTKGLAVIMVGIGMANASATSSYLSSQLVSSKNN---DTNMKKVLKECADKYGFAGDA 120

Query: 121 LRESLKDLGDETFDYAYMHVSAAADYANVCRDAFKGFPAVSYPTKLGRREEGLKRICRVV 180
           L+ S++DL  E++DYA MH++AAADY N C +AFK +PA++YP +L RREEGLK IC V 
Sbjct: 121 LQASVQDLASESYDYASMHINAAADYPNACHNAFKRYPALAYPPELARREEGLKHICDVA 180

Query: 181 LGILDLLGW 190
           LGI+D  GW
Sbjct: 181 LGIIDNFGW 181

The following BLAST results are available for this feature:

BLAST of Cucsa.100990 vs. Swiss-Prot


Match Name	E-value	Identity	Description
CVIF2_ARATH	1.6e-41	49.72	Cell wall / vacuolar inhibitor of fructosidase 2 OS=Arabidopsis thaliana GN=C/VI...	[more]
PLA1_PLAAC	9.6e-10	26.84	Putative invertase inhibitor OS=Platanus acerifolia PE=1 SV=1	[more]

BLAST of Cucsa.100990 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KJ03_CUCSA	8.2e-101	100.00	Uncharacterized protein OS=Cucumis sativus GN=Csa_6G483430 PE=4 SV=1	[more]
M5W386_PRUPE	6.0e-51	57.38	Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa021753mg PE=4 SV=1	[more]
W9SL32_9ROSA	1.6e-48	54.64	Pectinesterase inhibitor OS=Morus notabilis GN=L484_013831 PE=4 SV=1	[more]
A0A0R0JHD5_SOYBN	1.5e-46	52.49	Uncharacterized protein OS=Glycine max GN=GLYMA_06G152400 PE=4 SV=1	[more]
A0A0S3RF88_PHAAN	1.7e-45	51.38	Uncharacterized protein OS=Vigna angularis var. angularis GN=Vigan.02G207200 PE=...	[more]

BLAST of Cucsa.100990 vs. TAIR10


Match Name	E-value	Identity	Description
AT5G64620.1	9.1e-43	49.72	cell wall / vacuolar inhibitor of fructosidase 2	[more]
AT5G46940.1	3.3e-08	27.32	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT1G55770.1	8.1e-07	25.14	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT1G47960.1	5.2e-06	27.08	cell wall / vacuolar inhibitor of fructosidase 1	[more]

BLAST of Cucsa.100990 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|449461841\|ref\|XP_004148650.1\|	1.2e-100	100.00	PREDICTED: cell wall / vacuolar inhibitor of fructosidase 2 [Cucumis sativus]	[more]
gi\|659080910\|ref\|XP_008441045.1\|	5.3e-93	94.21	PREDICTED: cell wall / vacuolar inhibitor of fructosidase 2 [Cucumis melo]	[more]
gi\|595833188\|ref\|XP_007206622.1\|	8.6e-51	57.38	hypothetical protein PRUPE_ppa021753mg [Prunus persica]	[more]
gi\|658047079\|ref\|XP_008359213.1\|	2.8e-49	56.28	PREDICTED: cell wall / vacuolar inhibitor of fructosidase 2-like [Malus domestic...	[more]
gi\|645219391\|ref\|XP_008235482.1\|	3.6e-49	55.03	PREDICTED: cell wall / vacuolar inhibitor of fructosidase 2-like [Prunus mume]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR006501	Pectinesterase_inhib_dom

Vocabulary: **Molecular Function**
Term	Definition
GO:0004857	enzyme inhibitor activity

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0043086	negative regulation of catalytic activity
biological_process	GO:0005982	starch metabolic process
biological_process	GO:0005985	sucrose metabolic process
biological_process	GO:0008150	biological_process
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0004857	enzyme inhibitor activity
molecular_function	GO:0030599	pectinesterase activity

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cucsa.100990.1	Cucsa.100990.1	mRNA

InterPro

Analysis Name: InterPro Annotations of cucumber (Gy14)
Date Performed: 2017-01-17


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006501	Pectinesterase inhibitor domain	GENE3D	G3DSA:1.20.140.40		coord: 31..187 score: 5.3
IPR006501	Pectinesterase inhibitor domain	PFAM	PF04043	PMEI	coord: 35..182 score: 1.5
IPR006501	Pectinesterase inhibitor domain	SMART	SM00856	PMEI_2	coord: 31..183 score: 7.1
IPR006501	Pectinesterase inhibitor domain	TIGRFAMs	TIGR01614	TIGR01614	coord: 5..186 score: 6.9
IPR006501	Pectinesterase inhibitor domain	unknown	SSF101148	Plant invertase/pectin methylesterase inhibitor	coord: 33..187 score: 3.01
None	No IPR available	PANTHER	PTHR31080	FAMILY NOT NAMED	coord: 6..189 score: 1.5
None	No IPR available	PANTHER	PTHR31080:SF1	CELL WALL / VACUOLAR INHIBITOR OF FRUCTOSIDASE 2-RELATED	coord: 6..189 score: 1.5

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Cucsa.100990	Cla008440	Watermelon (97103) v1	cgywmB137
Cucsa.100990	Csa6G483430	Cucumber (Chinese Long) v2	cgycuB121
Cucsa.100990	MELO3C008049	Melon (DHL92) v3.5.1	cgymeB132
Cucsa.100990	ClCG01G015470	Watermelon (Charleston Gray)	cgywcgB134
Cucsa.100990	CSPI06G26530	Wild cucumber (PI 183967)	cgycpiB122
Cucsa.100990	CmoCh07G008200	Cucurbita moschata (Rifu)	cgycmoB0233
Cucsa.100990	Lsi01G011460	Bottle gourd (USVL1VR-Ls)	cgylsiB125
Cucsa.100990	Cp4.1LG10g03490	Cucurbita pepo (Zucchini)	cgycpeB0211
Cucsa.100990	Cp4.1LG19g12140	Cucurbita pepo (Zucchini)	cgycpeB0220
Cucsa.100990	MELO3C008049.2	Melon (DHL92) v3.6.1	cgymedB132
Cucsa.100990	CsaV3_6G041710	Cucumber (Chinese Long) v3	cgycucB126
Cucsa.100990	Bhi03G001051	Wax gourd	cgywgoB153
Cucsa.100990	Carg10539	Silver-seed gourd	carcgyB0280
Cucsa.100990	Carg05307	Silver-seed gourd	carcgyB0918

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Cucsa.100990	Cucurbita maxima (Rimu)	cgycmaB0231
Cucsa.100990	Cucurbita maxima (Rimu)	cgycmaB0235
Cucsa.100990	Cucurbita moschata (Rifu)	cgycmoB0227
Cucsa.100990	Watermelon (97103) v2	cgywmbB127