CSPI06G26530 (gene) Wild cucumber (PI 183967) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	CSPI06G26530
Type	gene
Organism	Cucumis sativus (Wild cucumber (PI 183967))
Description	Pectinesterase inhibitor
Location	Chr6 : 23397454 .. 23399061 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATATAAATTTAGCTTTTCATCATTCCTCCTCACATTCTCTTCACAGTTCTTTCTTAATTATTCCTTCTCTCTCTCTCTCTCTTTTCCCTCAATCTCTCCAATTTATCCAATCTCGAGATCACTCTCATAAGCCTTAGCCTTGCAGAGATAGTTGTTTTTTTTCTTAATACAGATCAGTGATATATATCTTCTTCATTCTCTTCCCTAACAAGAACTCTCATATCTTCCTTTTCGTAACCCTATCTACCATTTGGAAAACACCAAAGAAATTAAACCTTCTCTTTTTCATCTCTCTCACTCAAAAAGAAGTAATTTGAGGTATTTTTTTTATATCATTTTTCTCTCATTTTGTCCTATGAACATTGTGAGATTTTTCTTTAGAATGTTATAAGTGTCATATCTTCTTCCATTGTCCCTCTCTATATCTCTCTCTTAACACCCAAAAAGAGAACTTGAGAGAGTGCCTTATTCATTATCCTCTCTCTCTCTCTCTCTCTCTCTCTTTCCCACATTCAGAAAAAGGATAGACTCTCATATGGATATATGAAGATCTTGCTTTTCTCCAATCTCTCTACCATTTAAAACAACCAAAAAGAAAAAAACCATCTCCTTCATCTCTCTCTCCAAAAAGAAGTAATCTTTGAGGAAAATCTCCAATGGGTATGAAGAATTTCTCTATTTCTCTCATTTTCTTTGCAATCCCATTAATCTTTTTCCACAAAAATAATGGAGTTTCATTAGCATCAGCTGACCAAACCCTAATCCAAAAAACATGCACAAACACTCTTTACTACAAACTTTGCATGTCATCCCTTAAATCCGACCCCGCCAGCCTCACCGCCGACACCAAAGGCCTTGCCGTCATCATGGCTTCCATCGGTGCTGCTAACGCCACCGCCACCGCCAGCTACCTCTCGTCTCAGCTCCCTACATCCTCCTCCGGTGCCGCTGCCAACAACAACAAAACAAAATTGCTGAGACAATGCTCCGAGAAATACGCATTTGCAGCTGAAGCACTTAGAGAATCTTTGAAAGATTTGGGTGATGAGACTTTTGACTATGCTTATATGCATGTTTCTGCGGCGGCGGATTATGCCAATGTTTGCCGTGATGCCTTTAAAGGGTTCCCAGCGGTGAGTTATCCGACGAAGCTTGGCCGGAGAGAAGAAGGGTTGAAGCGTATTTGCAGAGTGGTTTTGGGGATTTTGGATCTTCTTGGCTGGTGATTTTCATCAATCATTATGTTCTCTCTCTAATCATATTTTTGTTTTTCTTTTTTTTACTTTTACTTTTTGCTTTTACTTTTTTTTGGGGTAATAAACTTGTTTGTCTTTTTAGGTGAAACGTTGATGATGCTTTTCTTTAAGGGATTTAATGACTCCTTTATTTGTTAAAATAATTTATTTCTTCTACAGTAAGATCTCATGTGTTTGAATTAGGTTTAGTAATGACACTTTTGATGTTGTAAACTTATAGAGGATTTTATATTTTTGTCATTCTTTTTTTAAGTTTTGAGTTATGATGGATAATATATAATAGGTCGAATCAAAACCTCTTATCTATCTAAGTTGTAATATTTTTAATTTTGTATGTTCGAATTTTTTGATA

mRNA sequence

ATGGGTATGAAGAATTTCTCTATTTCTCTCATTTTCTTTGCAATCCCATTAATCTTTTTCCACAAAAATAATGGAGTTTCATTAGCATCAGCTGACCAAACCCTAATCCAAAAAACATGCACAAACACTCTTTACTACAAACTTTGCATGTCATCCCTTAAATCCGACCCCGCCAGCCTCACCGCCGACACCAAAGGCCTTGCCGTCATCATGGCTTCCATCGGTGCTGCTAACGCCACCGCCACCGCCAGCTACCTCTCGTCTCAGCTCCCTACATCCTCCTCCGGTGCCGCTGCCAACAACAACAAAACAAAATTGCTGAGACAATGCTCCGAGAAATACGCATTTGCAGCTGAAGCACTTAGAGAATCTTTGAAAGATTTGGGTGATGAGACTTTTGACTATGCTTATATGCATGTTTCTGCGGCGGCGGATTATGCCAATGTTTGCCGTGATGCCTTTAAAGGGTTCCCAGCGGTGAGTTATCCGACGAAGCTTGGCCGGAGAGAAGAAGGGTTGAAGCGTATTTGCAGAGTGGTTTTGGGGATTTTGGATCTTCTTGGCTGGTGA

Coding sequence (CDS)

ATGGGTATGAAGAATTTCTCTATTTCTCTCATTTTCTTTGCAATCCCATTAATCTTTTTCCACAAAAATAATGGAGTTTCATTAGCATCAGCTGACCAAACCCTAATCCAAAAAACATGCACAAACACTCTTTACTACAAACTTTGCATGTCATCCCTTAAATCCGACCCCGCCAGCCTCACCGCCGACACCAAAGGCCTTGCCGTCATCATGGCTTCCATCGGTGCTGCTAACGCCACCGCCACCGCCAGCTACCTCTCGTCTCAGCTCCCTACATCCTCCTCCGGTGCCGCTGCCAACAACAACAAAACAAAATTGCTGAGACAATGCTCCGAGAAATACGCATTTGCAGCTGAAGCACTTAGAGAATCTTTGAAAGATTTGGGTGATGAGACTTTTGACTATGCTTATATGCATGTTTCTGCGGCGGCGGATTATGCCAATGTTTGCCGTGATGCCTTTAAAGGGTTCCCAGCGGTGAGTTATCCGACGAAGCTTGGCCGGAGAGAAGAAGGGTTGAAGCGTATTTGCAGAGTGGTTTTGGGGATTTTGGATCTTCTTGGCTGGTGA

Homology

[Close]

BLAST of CSPI06G26530 vs. Swiss-Prot
Match: CVIF2_ARATH (Cell wall / vacuolar inhibitor of fructosidase 2 OS=Arabidopsis thaliana GN=C/VIF2 PE=1 SV=1)

HSP 1 Score: 164.5 bits (415), Expect = 1.2e-39
Identity = 89/179 (49.72%), Postives = 118/179 (65.92%), Query Frame = 1


Query: 9   SLIFFAIPLIFFHKNNGVSLASADQTLIQKTCTNTLYYKLCMSSLKSDPASLTADTKGLA 68
           SLIF  +  + F  +  +S  S   T+I+ TC  T YYK C+S+LKSDP S TADTKGLA
Sbjct: 4   SLIFLLLVTLTFSASTLISAKSNTTTIIESTCKTTNYYKFCVSALKSDPRSPTADTKGLA 63

Query: 69  VIMASIGAANATATASYLSSQLPTSSSGAAANNNKTKLLRQCSEKYAFAAEALRESLKDL 128
            IM  +G  NAT+TA+Y++  L    S    +    K+L+ CSEKYA AA++LR +++DL
Sbjct: 64  SIMVGVGMTNATSTANYIAGNL----SATVKDTVLKKVLQDCSEKYALAADSLRLTIQDL 123

Query: 129 GDETFDYAYMHVSAAADYANVCRDAFKGFPAVSYPTKLGRREEGLKRICRVVLGILDLL 188
            DE +DYA MHV AA DY NVCR+ F+    ++YP ++ RRE  L+RIC VV GILD L
Sbjct: 124 DDEAYDYASMHVLAAQDYPNVCRNIFRRVKGLAYPVEIRRREASLRRICGVVSGILDRL 178

[Close]

BLAST of CSPI06G26530 vs. Swiss-Prot
Match: PMEI_ACTDE (Pectinesterase inhibitor OS=Actinidia deliciosa GN=PMEI PE=1 SV=2)

HSP 1 Score: 70.5 bits (171), Expect = 2.3e-11
Identity = 50/157 (31.85%), Postives = 82/157 (52.23%), Query Frame = 1


Query: 31  ADQTLIQKTCTNTLYYKLCMSSLKSDPASLTADTKGLAVIMASIGAANATATASYLSSQL 90
           A+  LI + C  T    LC+ +L+SDP S + D KGL      I  A+A  T+  ++S  
Sbjct: 32  AENHLISEICPKTRNPSLCLQALESDPRSASKDLKGLGQFSIDIAQASAKQTSKIIASLT 91

Query: 91  PTSSSGAAANNNKTKLLRQCSEKYAFAAEALRESLKDLGDETFDYAYMHVSAAADYANVC 150
             ++        +T     CSE YA A ++L ++ + L    ++   ++ SAA D A  C
Sbjct: 92  NQATDPKLKGRYET-----CSENYADAIDSLGQAKQFLTSGDYNSLNIYASAAFDGAGTC 151

Query: 151 RDAFKGFPAVSYPTKLGRREEGLKRICRVVLGILDLL 188
            D+F+G P +  PT+L + +  L+ +C +VL I +LL
Sbjct: 152 EDSFEGPPNI--PTQLHQADLKLEDLCDIVLVISNLL 181

[Close]

BLAST of CSPI06G26530 vs. Swiss-Prot
Match: PLA1_PLAAC (Putative invertase inhibitor OS=Platanus acerifolia PE=1 SV=1)

HSP 1 Score: 57.0 bits (136), Expect = 2.6e-07
Identity = 51/190 (26.84%), Postives = 89/190 (46.84%), Query Frame = 1


Query: 5   NFSISLIFFAIPLIFFHKNNGVSLASADQTLIQKTCTNT------LYYKLCMSSLKSDPA 64
           +FS+ + FF + L+        ++ SAD  ++Q TC         + Y  C+ SL +DP 
Sbjct: 4   SFSLCIFFFNLLLLL------QAVISAD--IVQGTCKKVAQRSPNVNYDFCVKSLGADPK 63

Query: 65  SLTADTKGLAVIMASIGAANATATASYLSSQLPTSSSGAAANNNKTKLLRQCSEKYAFAA 124
           S TAD +GL VI A++   + +   +++   L +    A       K L  C   YA A 
Sbjct: 64  SHTADLQGLGVISANLAIQHGSKIQTFIGRILKSKVDPAL-----KKYLNDCVGLYADAK 123

Query: 125 EALRESLKDLGDETFDYAYMHVSAAADYANVCRDAFKGFPAVSYPTKLGRREEGLKRICR 184
            +++E++ D   + +  A + +SAA D +  C D FK    +  P  + +  +   ++  
Sbjct: 124 SSVQEAIADFKSKDYASANVKMSAALDDSVTCEDGFKEKKGIVSP--VTKENKDYVQLTA 178

Query: 185 VVLGILDLLG 189
           + L I  LLG
Sbjct: 184 ISLAITKLLG 178

[Close]

BLAST of CSPI06G26530 vs. TrEMBL
Match: A0A0A0KJ03_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_6G483430 PE=4 SV=1)

HSP 1 Score: 364.0 bits (933), Expect = 1.1e-97
Identity = 188/189 (99.47%), Postives = 188/189 (99.47%), Query Frame = 1


Query: 1   MGMKNFSISLIFFAIPLIFFHKNNGVSLASADQTLIQKTCTNTLYYKLCMSSLKSDPASL 60
           MGMKNFSISLIFFAIPLIFFHKNNGVSLASADQTLIQKTCTNTLYYKLCMSSLKSDPASL
Sbjct: 1   MGMKNFSISLIFFAIPLIFFHKNNGVSLASADQTLIQKTCTNTLYYKLCMSSLKSDPASL 60

Query: 61  TADTKGLAVIMASIGAANATATASYLSSQLPTSSSGAAANNNKTKLLRQCSEKYAFAAEA 120
           TADTKGLAVIMASIGAANATATASYLSSQLPTSSSGA ANNNKTKLLRQCSEKYAFAAEA
Sbjct: 61  TADTKGLAVIMASIGAANATATASYLSSQLPTSSSGAGANNNKTKLLRQCSEKYAFAAEA 120

Query: 121 LRESLKDLGDETFDYAYMHVSAAADYANVCRDAFKGFPAVSYPTKLGRREEGLKRICRVV 180
           LRESLKDLGDETFDYAYMHVSAAADYANVCRDAFKGFPAVSYPTKLGRREEGLKRICRVV
Sbjct: 121 LRESLKDLGDETFDYAYMHVSAAADYANVCRDAFKGFPAVSYPTKLGRREEGLKRICRVV 180

Query: 181 LGILDLLGW 190
           LGILDLLGW
Sbjct: 181 LGILDLLGW 189

[Close]

BLAST of CSPI06G26530 vs. TrEMBL
Match: M5W386_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa021753mg PE=4 SV=1)

HSP 1 Score: 201.4 bits (511), Expect = 9.5e-49
Identity = 105/183 (57.38%), Postives = 133/183 (72.68%), Query Frame = 1


Query: 7   SISLIFFAIPLIFFHKNNGVSLASADQTLIQKTCTNTLYYKLCMSSLKSDPASLTADTKG 66
           SI    F + L F H+ +     +AD  LIQKTC NT YY LC+S+LKSDP S TADTKG
Sbjct: 3   SIIFSLFLLSLAFPHQTHNF-FVTADSNLIQKTCKNTKYYGLCVSTLKSDPTSKTADTKG 62

Query: 67  LAVIMASIGAANATATASYLSSQLPTSSSGAAANNNKTKLLRQCSEKYAFAAEALRESLK 126
           LAVIM  IG ANATAT+SYLSSQL +S +    + N  K+L++C++KY FA +AL+ S+K
Sbjct: 63  LAVIMVGIGMANATATSSYLSSQLVSSRNN---DTNMKKVLKECADKYGFAGDALQASVK 122

Query: 127 DLGDETFDYAYMHVSAAADYANVCRDAFKGFPAVSYPTKLGRREEGLKRICRVVLGILDL 186
           DL  E++DYA MH++AAADY N C +AFK +PA++YP +L RREEGLK IC V LGI+D 
Sbjct: 123 DLASESYDYASMHINAAADYPNACHNAFKRYPALAYPPELARREEGLKHICDVALGIIDN 181

Query: 187 LGW 190
            GW
Sbjct: 183 FGW 181

[Close]

BLAST of CSPI06G26530 vs. TrEMBL
Match: W9SL32_9ROSA (Pectinesterase inhibitor OS=Morus notabilis GN=L484_013831 PE=4 SV=1)

HSP 1 Score: 194.9 bits (494), Expect = 8.9e-47
Identity = 101/183 (55.19%), Postives = 136/183 (74.32%), Query Frame = 1


Query: 8   ISLIFFAIPLIFFHKNNGVSLASADQTLIQKTCTNTLYYKLCMSSLKSDPASLTA-DTKG 67
           +SL+  + P    H ++   LA+AD T IQ+TC  T +Y LC+SSLKSDP SL A D KG
Sbjct: 14  LSLLLLSFP----HGHHRRLLAAADATFIQQTCKTTKHYDLCVSSLKSDPTSLQASDAKG 73

Query: 68  LAVIMASIGAANATATASYLSSQLPTSSSGAAANNNKTKLLRQCSEKYAFAAEALRESLK 127
           LA IM +IG AN+TAT++Y+SSQLP++++  AA   K  + ++C++KY +AAEAL+ S +
Sbjct: 74  LANIMIAIGVANSTATSAYISSQLPSTTTTTAAAAKKKAVFKECADKYGYAAEALQASAQ 133

Query: 128 DLGDETFDYAYMHVSAAADYANVCRDAFKGFPAVSYPTKLGRREEGLKRICRVVLGILDL 187
           DL  E+FDY YMHV+AAADY N C +AF+ +P + YPT+L RRE+GLKRIC VVLGI+D 
Sbjct: 134 DLAAESFDYTYMHVTAAADYPNSCHNAFRRYPGLPYPTELARREDGLKRICDVVLGIIDN 192

Query: 188 LGW 190
           LGW
Sbjct: 194 LGW 192

[Close]

BLAST of CSPI06G26530 vs. TrEMBL
Match: A0A0R0JHD5_SOYBN (Uncharacterized protein OS=Glycine max GN=GLYMA_06G152400 PE=4 SV=1)

HSP 1 Score: 187.2 bits (474), Expect = 1.9e-44
Identity = 95/181 (52.49%), Postives = 128/181 (70.72%), Query Frame = 1


Query: 9   SLIFFAIPLIFFHKNNGVSLASADQTLIQKTCTNTLYYKLCMSSLKSDPASLTADTKGLA 68
           S IFF   L   H +   S+   D +LI++TC NT YY LC SSLKSDP+S  AD KGLA
Sbjct: 4   SKIFFLFLLFLAHLHQHASV-EGDSSLIKRTCKNTKYYNLCFSSLKSDPSSPNADPKGLA 63

Query: 69  VIMASIGAANATATASYLSSQLPTSSSGAAANNNKTKLLRQCSEKYAFAAEALRESLKDL 128
           VIM  IG  NAT+T+SYLSS+LPT S+    N    ++L++C++KY++A +AL++S++DL
Sbjct: 64  VIMIGIGMTNATSTSSYLSSKLPTPSN----NTTWKRVLKECADKYSYAGDALQDSVQDL 123

Query: 129 GDETFDYAYMHVSAAADYANVCRDAFKGFPAVSYPTKLGRREEGLKRICRVVLGILDLLG 188
            +E +DYAYMH++AA DY N C +AFK +P + YP  L RRE+GLK IC V +GI+D L 
Sbjct: 124 ANEAYDYAYMHITAAKDYPNACHNAFKRYPGLVYPRDLARREDGLKHICDVAMGIIDNLD 179

Query: 189 W 190
           W
Sbjct: 184 W 179

[Close]

BLAST of CSPI06G26530 vs. TrEMBL
Match: A0A0S3RF88_PHAAN (Uncharacterized protein OS=Vigna angularis var. angularis GN=Vigan.02G207200 PE=4 SV=1)

HSP 1 Score: 183.7 bits (465), Expect = 2.1e-43
Identity = 93/181 (51.38%), Postives = 130/181 (71.82%), Query Frame = 1


Query: 9   SLIFFAIPLIFFHKNNGVSLASADQTLIQKTCTNTLYYKLCMSSLKSDPASLTADTKGLA 68
           S IFF + L+F    +  +  + D TLI++TC NT YY LC SSLKSDP+S  AD KGLA
Sbjct: 3   SKIFFLLFLLFLLYPHQHACVNGDATLIKRTCKNTKYYNLCFSSLKSDPSSPNADPKGLA 62

Query: 69  VIMASIGAANATATASYLSSQLPTSSSGAAANNNKTKLLRQCSEKYAFAAEALRESLKDL 128
           +IM  IG +NAT+T+SYLSS+L + S+    N    +++++C++KY +A +AL+ S++DL
Sbjct: 63  MIMIGIGMSNATSTSSYLSSKLLSPSN----NTTLKRVIKECADKYTYAGDALQASVEDL 122

Query: 129 GDETFDYAYMHVSAAADYANVCRDAFKGFPAVSYPTKLGRREEGLKRICRVVLGILDLLG 188
            +E +DYAYMH++AA DY N C +AFK +PA+SYP  L RRE+GLK IC V +GI+D L 
Sbjct: 123 LNEAYDYAYMHITAAKDYPNACHNAFKRYPALSYPLDLARREDGLKHICDVAMGIIDNLD 179

Query: 189 W 190
           W
Sbjct: 183 W 179

[Close]

BLAST of CSPI06G26530 vs. TAIR10
Match: AT5G64620.1 (AT5G64620.1 cell wall / vacuolar inhibitor of fructosidase 2)

HSP 1 Score: 164.5 bits (415), Expect = 6.5e-41
Identity = 89/179 (49.72%), Postives = 118/179 (65.92%), Query Frame = 1


Query: 9   SLIFFAIPLIFFHKNNGVSLASADQTLIQKTCTNTLYYKLCMSSLKSDPASLTADTKGLA 68
           SLIF  +  + F  +  +S  S   T+I+ TC  T YYK C+S+LKSDP S TADTKGLA
Sbjct: 4   SLIFLLLVTLTFSASTLISAKSNTTTIIESTCKTTNYYKFCVSALKSDPRSPTADTKGLA 63

Query: 69  VIMASIGAANATATASYLSSQLPTSSSGAAANNNKTKLLRQCSEKYAFAAEALRESLKDL 128
            IM  +G  NAT+TA+Y++  L    S    +    K+L+ CSEKYA AA++LR +++DL
Sbjct: 64  SIMVGVGMTNATSTANYIAGNL----SATVKDTVLKKVLQDCSEKYALAADSLRLTIQDL 123

Query: 129 GDETFDYAYMHVSAAADYANVCRDAFKGFPAVSYPTKLGRREEGLKRICRVVLGILDLL 188
            DE +DYA MHV AA DY NVCR+ F+    ++YP ++ RRE  L+RIC VV GILD L
Sbjct: 124 DDEAYDYASMHVLAAQDYPNVCRNIFRRVKGLAYPVEIRRREASLRRICGVVSGILDRL 178

[Close]

BLAST of CSPI06G26530 vs. TAIR10
Match: AT5G46940.1 (AT5G46940.1 Plant invertase/pectin methylesterase inhibitor superfamily protein)

HSP 1 Score: 48.1 bits (113), Expect = 6.8e-06
Identity = 50/183 (27.32%), Postives = 81/183 (44.26%), Query Frame = 1


Query: 12  FFAIPLIFFHKNNGVSLASADQTLIQKTCTNT------LYYKLCMSSLKSDPASLTA-DT 71
           F    + FF  +NG++     QTLI+ +C           Y LC++SL+++P + TA D 
Sbjct: 3   FLLYLVTFFVLSNGLANG---QTLIRNSCKKATATSPKFKYNLCVTSLETNPQAKTAKDL 62

Query: 72  KGLAVIMASIGAANATATASYLSSQLPTSSSGAAANNNKTKLLRQCSEKYAFAAEALRES 131
            GL  +MAS    NA   A+ L   +     G   N      LR C + Y  A  +L E+
Sbjct: 63  AGL--VMAS--TKNAVTKATTLKGTVDKIIKGKKVNKMTAMPLRDCLQLYTDAIGSLNEA 122

Query: 132 LKDLGDETFDYAYMHVSAAADYANVCRDAFKGFPAVSYPTKLGRREEGLKRICRVVLGIL 188
           L  +    +      +SAA D  + C   FK   A   P+ + +  + L ++  + L   
Sbjct: 123 LAGVKSRNYPTVKTVLSAAMDTPSTCETGFKERKA---PSPVTKENDNLYQMILIPLAFT 175

[Close]

BLAST of CSPI06G26530 vs. NCBI nr
Match: gi|449461841|ref|XP_004148650.1| (PREDICTED: cell wall / vacuolar inhibitor of fructosidase 2 [Cucumis sativus])

HSP 1 Score: 364.0 bits (933), Expect = 1.6e-97
Identity = 188/189 (99.47%), Postives = 188/189 (99.47%), Query Frame = 1


Query: 1   MGMKNFSISLIFFAIPLIFFHKNNGVSLASADQTLIQKTCTNTLYYKLCMSSLKSDPASL 60
           MGMKNFSISLIFFAIPLIFFHKNNGVSLASADQTLIQKTCTNTLYYKLCMSSLKSDPASL
Sbjct: 1   MGMKNFSISLIFFAIPLIFFHKNNGVSLASADQTLIQKTCTNTLYYKLCMSSLKSDPASL 60

Query: 61  TADTKGLAVIMASIGAANATATASYLSSQLPTSSSGAAANNNKTKLLRQCSEKYAFAAEA 120
           TADTKGLAVIMASIGAANATATASYLSSQLPTSSSGA ANNNKTKLLRQCSEKYAFAAEA
Sbjct: 61  TADTKGLAVIMASIGAANATATASYLSSQLPTSSSGAGANNNKTKLLRQCSEKYAFAAEA 120

Query: 121 LRESLKDLGDETFDYAYMHVSAAADYANVCRDAFKGFPAVSYPTKLGRREEGLKRICRVV 180
           LRESLKDLGDETFDYAYMHVSAAADYANVCRDAFKGFPAVSYPTKLGRREEGLKRICRVV
Sbjct: 121 LRESLKDLGDETFDYAYMHVSAAADYANVCRDAFKGFPAVSYPTKLGRREEGLKRICRVV 180

Query: 181 LGILDLLGW 190
           LGILDLLGW
Sbjct: 181 LGILDLLGW 189

[Close]

BLAST of CSPI06G26530 vs. NCBI nr
Match: gi|659080910|ref|XP_008441045.1| (PREDICTED: cell wall / vacuolar inhibitor of fructosidase 2 [Cucumis melo])

HSP 1 Score: 340.9 bits (873), Expect = 1.4e-90
Identity = 179/190 (94.21%), Postives = 184/190 (96.84%), Query Frame = 1


Query: 1   MGMKNFSISLIFFAIPLIFFHKNNGVSLASADQTLIQKTCTNTLYYKLCMSSLKSDPASL 60
           MGMKNFSISLIFFAIPLIFFHKN GVSLASADQTLIQKTCTNTLYYKLCMSSLKSDP+SL
Sbjct: 1   MGMKNFSISLIFFAIPLIFFHKN-GVSLASADQTLIQKTCTNTLYYKLCMSSLKSDPSSL 60

Query: 61  TADTKGLAVIMASIGAANATATASYLSSQLPTSSSGAA-ANNNKTKLLRQCSEKYAFAAE 120
           TADTKGLA+IMASIGAANATAT++YLSSQLPTSSS A  ANNNKTKLLRQCSEKYAFAAE
Sbjct: 61  TADTKGLAIIMASIGAANATATSTYLSSQLPTSSSAATNANNNKTKLLRQCSEKYAFAAE 120

Query: 121 ALRESLKDLGDETFDYAYMHVSAAADYANVCRDAFKGFPAVSYPTKLGRREEGLKRICRV 180
           ALRESLKDL DET+DYAYMHVSAAADYANVCRDAFKGFPAVSYP KLGRREEGLKRICRV
Sbjct: 121 ALRESLKDLADETYDYAYMHVSAAADYANVCRDAFKGFPAVSYPAKLGRREEGLKRICRV 180

Query: 181 VLGILDLLGW 190
           VLGILDLLGW
Sbjct: 181 VLGILDLLGW 189

[Close]

BLAST of CSPI06G26530 vs. NCBI nr
Match: gi|595833188|ref|XP_007206622.1| (hypothetical protein PRUPE_ppa021753mg [Prunus persica])

HSP 1 Score: 201.4 bits (511), Expect = 1.4e-48
Identity = 105/183 (57.38%), Postives = 133/183 (72.68%), Query Frame = 1


Query: 7   SISLIFFAIPLIFFHKNNGVSLASADQTLIQKTCTNTLYYKLCMSSLKSDPASLTADTKG 66
           SI    F + L F H+ +     +AD  LIQKTC NT YY LC+S+LKSDP S TADTKG
Sbjct: 3   SIIFSLFLLSLAFPHQTHNF-FVTADSNLIQKTCKNTKYYGLCVSTLKSDPTSKTADTKG 62

Query: 67  LAVIMASIGAANATATASYLSSQLPTSSSGAAANNNKTKLLRQCSEKYAFAAEALRESLK 126
           LAVIM  IG ANATAT+SYLSSQL +S +    + N  K+L++C++KY FA +AL+ S+K
Sbjct: 63  LAVIMVGIGMANATATSSYLSSQLVSSRNN---DTNMKKVLKECADKYGFAGDALQASVK 122

Query: 127 DLGDETFDYAYMHVSAAADYANVCRDAFKGFPAVSYPTKLGRREEGLKRICRVVLGILDL 186
           DL  E++DYA MH++AAADY N C +AFK +PA++YP +L RREEGLK IC V LGI+D 
Sbjct: 123 DLASESYDYASMHINAAADYPNACHNAFKRYPALAYPPELARREEGLKHICDVALGIIDN 181

Query: 187 LGW 190
            GW
Sbjct: 183 FGW 181

[Close]

BLAST of CSPI06G26530 vs. NCBI nr
Match: gi|645219391|ref|XP_008235482.1| (PREDICTED: cell wall / vacuolar inhibitor of fructosidase 2-like [Prunus mume])

HSP 1 Score: 196.8 bits (499), Expect = 3.4e-47
Identity = 104/189 (55.03%), Postives = 133/189 (70.37%), Query Frame = 1


Query: 1   MGMKNFSISLIFFAIPLIFFHKNNGVSLASADQTLIQKTCTNTLYYKLCMSSLKSDPASL 60
           MG   FS+ L+  A P             +AD  LIQKTC NT YY LC+S+LKSDP S 
Sbjct: 1   MGSIIFSLFLLSLAFP-----PQTHNFFVTADSNLIQKTCKNTKYYGLCVSTLKSDPTSK 60

Query: 61  TADTKGLAVIMASIGAANATATASYLSSQLPTSSSGAAANNNKTKLLRQCSEKYAFAAEA 120
           TADTKGLAVIM  IG ANA+AT+SYLSSQL +S +    + N  K+L++C++KY FA +A
Sbjct: 61  TADTKGLAVIMVGIGMANASATSSYLSSQLVSSKNN---DTNMKKVLKECADKYGFAGDA 120

Query: 121 LRESLKDLGDETFDYAYMHVSAAADYANVCRDAFKGFPAVSYPTKLGRREEGLKRICRVV 180
           L+ S++DL  E++DYA MH++AAADY N C +AFK +PA++YP +L RREEGLK IC V 
Sbjct: 121 LQASVQDLASESYDYASMHINAAADYPNACHNAFKRYPALAYPPELARREEGLKHICDVA 180

Query: 181 LGILDLLGW 190
           LGI+D  GW
Sbjct: 181 LGIIDNFGW 181

[Close]

BLAST of CSPI06G26530 vs. NCBI nr
Match: gi|658047079|ref|XP_008359213.1| (PREDICTED: cell wall / vacuolar inhibitor of fructosidase 2-like [Malus domestica])

HSP 1 Score: 195.7 bits (496), Expect = 7.5e-47
Identity = 103/183 (56.28%), Postives = 132/183 (72.13%), Query Frame = 1


Query: 6   FSISLIFFAIPLIFFHKNNGVSLASADQTLIQKTCTNTLYYKLCMSSLKSDPASLTADTK 65
           +S   + F +   F H+N    L  AD TLIQ TC NT YY LC+SSLKS P S  ADTK
Sbjct: 43  YSTIFLLFLLTTTFPHQNPNF-LVHADSTLIQNTCKNTKYYGLCVSSLKSVPTSKNADTK 102

Query: 66  GLAVIMASIGAANATATASYLSSQLPTSSSGAAANNNKTKLLRQCSEKYAFAAEALRESL 125
           GLAVIM  IG ANATAT+SYLSSQL +SS+    + N  K+L++C++KY +A +AL+ S+
Sbjct: 103 GLAVIMVGIGMANATATSSYLSSQLISSST---TDTNLKKVLKECADKYGYAGDALQASV 162

Query: 126 KDLGDETFDYAYMHVSAAADYANVCRDAFKGFPAVSYPTKLGRREEGLKRICRVVLGILD 185
           +DLG E++DYAYMH++AAADY N C +AFK +P ++YP +L RREEGLK IC V LGI+D
Sbjct: 163 QDLGSESYDYAYMHITAAADYPNACHNAFKRYPGLAYPPELARREEGLKHICDVALGIID 221

Query: 186 LLG 189
             G
Sbjct: 223 NFG 221

The following BLAST results are available for this feature:

BLAST of CSPI06G26530 vs. Swiss-Prot


Match Name	E-value	Identity	Description
CVIF2_ARATH	1.2e-39	49.72	Cell wall / vacuolar inhibitor of fructosidase 2 OS=Arabidopsis thaliana GN=C/VI...	[more]
PMEI_ACTDE	2.3e-11	31.85	Pectinesterase inhibitor OS=Actinidia deliciosa GN=PMEI PE=1 SV=2	[more]
PLA1_PLAAC	2.6e-07	26.84	Putative invertase inhibitor OS=Platanus acerifolia PE=1 SV=1	[more]

BLAST of CSPI06G26530 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KJ03_CUCSA	1.1e-97	99.47	Uncharacterized protein OS=Cucumis sativus GN=Csa_6G483430 PE=4 SV=1	[more]
M5W386_PRUPE	9.5e-49	57.38	Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa021753mg PE=4 SV=1	[more]
W9SL32_9ROSA	8.9e-47	55.19	Pectinesterase inhibitor OS=Morus notabilis GN=L484_013831 PE=4 SV=1	[more]
A0A0R0JHD5_SOYBN	1.9e-44	52.49	Uncharacterized protein OS=Glycine max GN=GLYMA_06G152400 PE=4 SV=1	[more]
A0A0S3RF88_PHAAN	2.1e-43	51.38	Uncharacterized protein OS=Vigna angularis var. angularis GN=Vigan.02G207200 PE=...	[more]

BLAST of CSPI06G26530 vs. TAIR10


Match Name	E-value	Identity	Description
AT5G64620.1	6.5e-41	49.72	cell wall / vacuolar inhibitor of fructosidase 2	[more]
AT5G46940.1	6.8e-06	27.32	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]

BLAST of CSPI06G26530 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|449461841\|ref\|XP_004148650.1\|	1.6e-97	99.47	PREDICTED: cell wall / vacuolar inhibitor of fructosidase 2 [Cucumis sativus]	[more]
gi\|659080910\|ref\|XP_008441045.1\|	1.4e-90	94.21	PREDICTED: cell wall / vacuolar inhibitor of fructosidase 2 [Cucumis melo]	[more]
gi\|595833188\|ref\|XP_007206622.1\|	1.4e-48	57.38	hypothetical protein PRUPE_ppa021753mg [Prunus persica]	[more]
gi\|645219391\|ref\|XP_008235482.1\|	3.4e-47	55.03	PREDICTED: cell wall / vacuolar inhibitor of fructosidase 2-like [Prunus mume]	[more]
gi\|658047079\|ref\|XP_008359213.1\|	7.5e-47	56.28	PREDICTED: cell wall / vacuolar inhibitor of fructosidase 2-like [Malus domestic...	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR006501	Pectinesterase_inhib_dom

Vocabulary: **Molecular Function**
Term	Definition
GO:0004857	enzyme inhibitor activity

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0043086	negative regulation of catalytic activity
biological_process	GO:0005982	starch metabolic process
biological_process	GO:0005985	sucrose metabolic process
biological_process	GO:0080167	response to karrikin
biological_process	GO:0008150	biological_process
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0004857	enzyme inhibitor activity
molecular_function	GO:0030599	pectinesterase activity

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI06G26530.1	CSPI06G26530.1	mRNA

InterPro

Analysis Name: InterPro Annotations of cucumber (PI183967)
Date Performed: 2017-01-17


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006501	Pectinesterase inhibitor domain	GENE3D	G3DSA:1.20.140.40		coord: 31..187 score: 7.5
IPR006501	Pectinesterase inhibitor domain	PFAM	PF04043	PMEI	coord: 35..182 score: 2.2
IPR006501	Pectinesterase inhibitor domain	SMART	SM00856	PMEI_2	coord: 31..183 score: 1.0
IPR006501	Pectinesterase inhibitor domain	TIGRFAMs	TIGR01614	TIGR01614	coord: 5..186 score: 7.6
IPR006501	Pectinesterase inhibitor domain	unknown	SSF101148	Plant invertase/pectin methylesterase inhibitor	coord: 33..187 score: 3.14
None	No IPR available	PANTHER	PTHR31080	FAMILY NOT NAMED	coord: 6..189 score: 1.8
None	No IPR available	PANTHER	PTHR31080:SF1	CELL WALL / VACUOLAR INHIBITOR OF FRUCTOSIDASE 2-RELATED	coord: 6..189 score: 1.8

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
CSPI06G26530	Cla008440	Watermelon (97103) v1	cpiwmB521
CSPI06G26530	Csa6G483430	Cucumber (Chinese Long) v2	cpicuB313
CSPI06G26530	MELO3C008049	Melon (DHL92) v3.5.1	cpimeB513
CSPI06G26530	ClCG01G015470	Watermelon (Charleston Gray)	cpiwcgB453
CSPI06G26530	Lsi01G011460	Bottle gourd (USVL1VR-Ls)	cpilsiB439
CSPI06G26530	MELO3C008049.2	Melon (DHL92) v3.6.1	cpimedB497
CSPI06G26530	CsaV3_6G041710	Cucumber (Chinese Long) v3	cpicucB363
CSPI06G26530	Bhi03G001051	Wax gourd	cpiwgoB628
CSPI06G26530	Cucsa.100990	Cucumber (Gy14) v1	cgycpiB122
CSPI06G26530	CmoCh07G008200	Cucurbita moschata (Rifu)	cmocpiB873
CSPI06G26530	Cp4.1LG19g12140	Cucurbita pepo (Zucchini)	cpecpiB503
CSPI06G26530	Cp4.1LG10g03490	Cucurbita pepo (Zucchini)	cpecpiB080
CSPI06G26530	CsGy6G025980	Cucumber (Gy14) v2	cgybcpiB287
CSPI06G26530	Carg10539	Silver-seed gourd	carcpiB0271
CSPI06G26530	Carg05307	Silver-seed gourd	carcpiB0846

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
CSPI06G26530	Watermelon (97103) v2	cpiwmbB433
CSPI06G26530	Cucurbita maxima (Rimu)	cmacpiB689
CSPI06G26530	Cucurbita maxima (Rimu)	cmacpiB893
CSPI06G26530	Cucurbita moschata (Rifu)	cmocpiB681