Cucsa.065490 (gene) Cucumber (Gy14) v1

Overview
NameCucsa.065490
Typegene
OrganismCucumis sativus (Cucumber (Gy14) v1)
DescriptionE3 ubiquitin ligase BIG BROTHER
Locationscaffold00696 : 210453 .. 214613 (+)
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AATAAATTAACTGCTTGAAGTTATGTCAAGAATAGGTTTGGAGAACTTTCAGGACGCTTAGGTTTGTTACTGCTAGTGTATTTCCCCTTTGGGCTCTGATTTTTATTTTTGCTACGTTACTTGTGCCTGAAGCAAATACTTATTGAAACAGAATTGTCTAAAATGAGCTGGAACTCAAACATCGAGGTTCATTGCATGAATAACGGTTATCCTTATAGTACTGCTTCTTTTATGGAATACTTTGAAGGTCTTACGTATGATCATGTGAATTTCATTTTTtCGGGTGCCTCACATTCTCAGGTACTATAAATCTTTTTtATTTTAGATGCTCTCCATGAATGTCTTGTCTGGAAGCTGGACTTATAGAACTTGTGGCATTTAAATGCTGCTTTATCTTTACCTTATAGACTTGATTCTCTGTTCTGTAGATTTTTTGACAATTGTCTCAAGTAAAAAaGGGGAAATCATGTGACATTTCATCTTCTAACTACTTTTGGCTTCTCAACTATGGCCTTTTCATTTTGCTTTAACTTCCTGATGCATGCTTGATTTGACATTTATTCTCAAGATCAAAGGGTCTTTATATCTGATTTTCATGATTCCAAATTAATAAACTTATTTTCCCGACATGGAAGTATAATATTTTAATTTCATTCAGGAGACTGTTTGTTCATCAACTAACTCAAGTTACAAGTTTGGGCATTCTGATTTATGGAGCGCATCATACTTTGATGCTCAATCGTTTGAGATTCAAGATCATGAATCCACTATTGATGAACACAGGAGGCCGCTCTTGACAGTCCAGAATGAACAGAGTTCAGGAAATAGCGTGTGGGAAGAAAATGCCAATCCTAATATGTCCGGTAACAGCATGGAATGTAAGGGTTAATGCCTGTTTTGTTTCAAGCTTTTGAATGAACACTATAGTATGATTGTTTGTTAAACAGAAGTTTTCAAATAGTATGATTGTTTGTTAAACAGAAGTTGTCAAGTTAAGGTAATGGAACAAACATTCAGATATTGAATGTTACGGTTCTATAAAATCACACGCTGCAAAGAAGAGATACTTATTGACTCTTTATTTAGATTATTTTTCTCAGTTCATGAATGGAGGCATTTGTAAGAGAAAGCAGTTGATGTCATCCATTTCTGGTCAATCGACAAACTTCATTTTTAGGCTGTTTATTATTTCTCTCAGTTCATAAATGATAGTGCAATATGGTTCTGAGGGTTTTCTTATGCTACATGTAGAAAAATTGGCTTGTGCCATATTAGTCATGTTGTAAGATGGGTAACTTATTCAATGCACGAAATCAAATTTAGGGTGACTTAACGAGTATCTTGTAGATGAGATACAATCTTTCTCAGTTCATAAACGATAATGTAAAATATGTTCATTTGTTTTCTTATGCTACACATAGGAAAATGGCTTTAGACGTAGTAGTCATGCTGTAAAGTGGGAAGCTTATTTGATGCAACAATTGAATTTGGGGTGATTTAATGAGTATCCTGTAAATGAGATACAATCGCACCATCCATTTATACACATATCTTCTATCATATTTCAACGTTAACTTGTAATTACTTGGTGCATCTCATTTTACCAAGTACTGTTCTGTTGTAGTATTAACTATCCTGCTAGGTATAGTACCAAAGATATGCACAAAATCTAACAGCAAAATCATAATACAGAAAAATCCTATTTTATATCATTTTATTACTCATGATTGTTTGCTCCTTTTCTTCTTTCTGATTCAATTATAATGTTTATGGAAATTTAAATACCAGTTCCTAAACTCCGTTTTTCTTTATTACTTTTTCAGGCCCTCGGAGGCATTCAAATTATCATGAGTATCAGGTCTGCAGCTTATCGATTCATAAACATGTTATGCCACTAAGTTTCAGTTGTAGTGTTAAGGTTGTTTTCGGTGGAGTACATTTTTGGTTAGTGTCCTGTATTCTGTGGTTCATGCTGGCAACCAGTCACATACTTGTGTTAATACATAAAATAAAATTATTCCTGTAAGATATAAAACTGGCTTATCTGAAATCACAGAATAAAGGAAAAGATAGAGGATATTAGAATACAAATTCATTTCTAATCCAGTGGTGACAAACTTGGTGGCGGTTCAACTCCCTTAACATCTATTATTCCAGATTTTCTATTCCGCGCCAAATAGACCTGGAATTTACAGTTCCAATGCTTCAGAAGACAACTATAAATGCATGATTCCTAATCTGTTCCATCTTGAAATGTGCAGACTATTTGGCAAGAGATTGTTGATCCTGATAACATGACTTATGAGGTTAGAGCAGCTGATTTTCTTTCCTATTTACTTTTGTATATTACTGAACAAAATGTTTTCTCCTGTAACTTTATATGTTCTTATTGAAGCTATATTTACTTCAATGGCTATTTATTATGATGGATATTGCTAATTGAAATGTTATATTACGGCCAGTCAGTAAGAAGTCTTGCTGCTGTGTAGCAAAACCAGTAGCCATTACAGCTAATAGGCTAGGTGTTGGACGCTTTCTTGCTAAGGTAGAAAGGATAGAAATGATGGCAGAAGTTAACCTAATTGTTTATTCTGCGTTTTGGTTGTTGGTACACTTCGACGTAATTTTCTATCCTTGAACTTGGTTTCTTGGAGGGCCTTACATATATACCAAAGAAAGCTTGCTTCTTTGGAGCGCTCTGCCTGTTGACGCTGCTTTTTAAGAAGCTAAAATTGATTAGATAGGAGCATAGCTGCATAAGTAATCAGCTGTAAATCCACTCAATACATTCTGAAATCAAGTTCCATGTTTAATAAACGTAAAATTTGTCTGGCTAACTGTTATATGTAAAGTCAACTATATGCCTCCTGAATTATGTCGATTACTCTCTTCAATAAAGTGACGGTTTAAAGAAACTTATTTCAGGAATTAGTAGATTTAGGTGAGACAGTAGGAACTCAAAGTAGAGGCCTTACACAAGAACTAATTGCATTGCTTCCAGTATCGAGGTACAGATGGGGATTTTtCTCAAGGAAGAAATCACGAAATGAAAGGTAACACTAGATTGATTGATCATCTTATCTCGAAATATTTTAAACTTTGTATTATTTTtCGGTTGTTTTTtAGAATTGTTCCAGCTATCAAGATGTAGTAATTCTCACCAATGCCCTTATACTTAGTAACGCCTATAACAAGTGGGGTATCGGGGGGTTTGATAAGCAACACATAATAATTATTGCCAAGCTATGTTTATTTTGACTTGTGTACCTCATATTTTCATTTTCATTATTATTGTTAAGAACAATATACAGTTACGATGAATGATCTTTCTCAATATTATCTCATTTACTCAAAATCATCTCAATAAAGAGAGAAAACAAGTGGTTTGGCCGACTAGCCTGCCTGCTACTTGCAACGCCACCCTTTATTCAATGGGGAAGAAGGAAAACGATCAATAGATTCAAATTGGTGCACAAGCCTTCCTCATTAACTTTTTTGAGGAATTACCGATTGACAATGATATATTTCTTGCATTAATTTTTTTAACATTATATATATGAAGACACATCTTGATTTGTATACAGGTGTGTGATATGCCAAATGGAATACAAACGTGGGGATAAAATAATTACTCTACCATGCAAACACAAGTACCATACAAGTTGCGGGACCAAGTGGCTTAGTATCAAGAAGGTGACTTTCTGATATCTTTTATCCCTCTTTTAGTAAAACATACAATCAGCAATTTTACTCAATATTGCATAATCTCTCTCCTCCAGGCATGTCCCATTTGCTACACTGAGGTTTTTGGTGACACTTCAAAACGATAAAGAAAAGAAGGAAAaGAAAAAAAaGGCTTTGATTAATTGTCATGAGAAAGGGAACCCTTAAAAAGGTTTGGAGTATACTTATTTCGTTTCCTTTCTCATTTACAACTTTACACAATCGTCGTTTGATGTTAAAGATTAGGAAAAATCTTTGATTTCAATACAGAGGATGATAGAGCCAGATAGTAGAATTTGATTGAAATCAATTTGTTTGTTTGTTTGTTTGGTTTTGTTTTTGTTATTTTTTCAACTTGGCTCATATTGAACCACGACGACTTGGTTTTTGGCTCTACCGATTATAAATATTTCTTATTAGTTGGAGCCTGGAGGGGGC

mRNA sequence

AATAAATTAACTGCTTGAAGTTATGTCAAGAATAGGTTTGGAGAACTTTCAGGACGCTTAGGAGACTGTTTGTTCATCAACTAACTCAAGTTACAAGTTTGGGCATTCTGATTTATGGAGCGCATCATACTTTGATGCTCAATCGTTTGAGATTCAAGATCATGAATCCACTATTGATGAACACAGGAGGCCGCTCTTGACAGTCCAGAATGAACAGAGTTCAGGAAATAGCGTGTGGGAAGAAAATGCCAATCCTAATATGTCCGGTAACAGCATGGAATGCCCTCGGAGGCATTCAAATTATCATGAGTATCAGACTATTTGGCAAGAGATTGTTGATCCTGATAACATGACTTATGAGGAATTAGTAGATTTAGGTGAGACAGTAGGAACTCAAAGTAGAGGCCTTACACAAGAACTAATTGCATTGCTTCCAGTATCGAGGTACAGATGGGGATTTTTCTCAAGGAAGAAATCACGAAATGAAAGGTGTGTGATATGCCAAATGGAATACAAACGTGGGGATAAAATAATTACTCTACCATGCAAACACAAGTACCATACAAGTTGCGGGACCAAGTGGCTTAGTATCAAGAAGGCATGTCCCATTTGCTACACTGAGGTTTTTGGTGACACTTCAAAACGATAAAGAAAAGAAGGAAAAGAAAAAAAAGGCTTTGATTAATTGTCATGAGAAAGGGAACCCTTAAAAAGGTTTGGAGTATACTTATTTCGTTTCCTTTCTCATTTACAACTTTACACAATCGTCGTTTGATGTTAAAGATTAGGAAAAATCTTTGATTTCAATACAGAGGATGATAGAGCCAGATAGTAGAATTTGATTGAAATCAAtttgtttgtttgtttgtttgGTTTTGTTTTTGTTATTTTTTCAACTTGGCTCATATTGAACCACGACGACTTGGTTTTTGGCTCTACCGATTATAAATATTTCTTATTAGTTGGAGCCTGGAGGGGGC

Coding sequence (CDS)

ATGTCCGGTAACAGCATGGAATGCCCTCGGAGGCATTCAAATTATCATGAGTATCAGACTATTTGGCAAGAGATTGTTGATCCTGATAACATGACTTATGAGGAATTAGTAGATTTAGGTGAGACAGTAGGAACTCAAAGTAGAGGCCTTACACAAGAACTAATTGCATTGCTTCCAGTATCGAGGTACAGATGGGGATTTTtCTCAAGGAAGAAATCACGAAATGAAAGGTGTGTGATATGCCAAATGGAATACAAACGTGGGGATAAAATAATTACTCTACCATGCAAACACAAGTACCATACAAGTTGCGGGACCAAGTGGCTTAGTATCAAGAAGGCATGTCCCATTTGCTACACTGAGGTTTTTGGTGACACTTCAAAACGATAA

Protein sequence

MSGNSMECPRRHSNYHEYQTIWQEIVDPDNMTYEELVDLGETVGTQSRGLTQELIALLPVSRYRWGFFSRKKSRNERCVICQMEYKRGDKIITLPCKHKYHTSCGTKWLSIKKACPICYTEVFGDTSKR*
Homology
[Close]
BLAST of Cucsa.065490 vs. Swiss-Prot
Match: BB_ARATH (E3 ubiquitin ligase BIG BROTHER OS=Arabidopsis thaliana GN=BB PE=1 SV=1)

HSP 1 Score: 149.8 bits (377), Expect = 2.0e-35
Identity = 66/121 (54.55%), Postives = 86/121 (71.07%), Query Frame = 1 

Query: 7   ECPRRHSNYHEYQTIWQEIVDPDNMTYEELVDLGETVGTQSRGLTQELIALLPVSRYRWG 66
           EC     +    Q  WQ+ +DPD MTYEELV+LGE VGT+SRGL+QELI  LP  +Y++G
Sbjct: 126 ECIANEHDPDSPQVSWQDDIDPDTMTYEELVELGEAVGTESRGLSQELIETLPTKKYKFG 185

Query: 67  FFSRKKSRNERCVICQMEYKRGDKIITLPCKHKYHTSCGTKWLSIKKACPICYTEVFGDT 126
               +K   ERCVICQ++YK G++ + LPCKH YH+ C +KWLSI K CP+C +EVFG+ 
Sbjct: 186 SIFSRKRAGERCVICQLKYKIGERQMNLPCKHVYHSECISKWLSINKVCPVCNSEVFGEP 245

Query: 127 S 128
           S
Sbjct: 246 S 246

[Close]
BLAST of Cucsa.065490 vs. Swiss-Prot
Match: BBR_ARATH (E3 ubiquitin ligase BIG BROTHER-related OS=Arabidopsis thaliana GN=BBR PE=2 SV=1)

HSP 1 Score: 117.1 bits (292), Expect = 1.5e-25
Identity = 54/112 (48.21%), Postives = 72/112 (64.29%), Query Frame = 1 

Query: 16  HEYQTIWQEIVDPDNMTYEELVDLGETVGTQSRGLTQELIALLPVSRYRWGFFSRKKSRN 75
           H  Q  W E+ DPD ++YEEL+ LG+ VGT+SRGL+ + IA LP  RY+ G    +   N
Sbjct: 229 HTSQDAWDEM-DPDELSYEELLALGDIVGTESRGLSADTIASLPSKRYKEG--DNQNGTN 288

Query: 76  ERCVICQMEYKRGDKIITLPCKHKYHTSCGTKWLSIKKACPICYTEVFGDTS 128
           E CVIC+++Y+  + +I LPCKH YH+ C   WL I K CP+C  EV   TS
Sbjct: 289 ESCVICRLDYEDDEDLILLPCKHSYHSECINNWLKINKVCPVCSAEVSTSTS 337

[Close]
BLAST of Cucsa.065490 vs. Swiss-Prot
Match: MBR2_ARATH (E3 ubiquitin-protein ligase MBR2 OS=Arabidopsis thaliana GN=MBR2 PE=1 SV=1)

HSP 1 Score: 86.7 bits (213), Expect = 2.1e-16
Identity = 40/95 (42.11%), Postives = 56/95 (58.95%), Query Frame = 1 

Query: 26  VDPDNMTYEELVDLGETVGTQSRGLTQELIALLPVSRYRWGFFSRKKSRNERCVICQMEY 85
           +D DNM+YEEL+ LGE +G  S GL++E+I  +          +      E C +CQ EY
Sbjct: 567 LDVDNMSYEELLALGERIGDVSTGLSEEVILKVMKQHKHTSSAAGSHQDMEPCCVCQEEY 626

Query: 86  KRGDKIITLPCKHKYHTSCGTKWLSIKKACPICYT 121
             GD + TL C H++HT+C  +WL +K  CPIC T
Sbjct: 627 AEGDDLGTLGCGHEFHTACVKQWLMLKNLCPICKT 661

[Close]
BLAST of Cucsa.065490 vs. Swiss-Prot
Match: MBR1_ARATH (E3 ubiquitin-protein ligase MBR1 OS=Arabidopsis thaliana GN=MBR1 PE=1 SV=1)

HSP 1 Score: 82.4 bits (202), Expect = 4.0e-15
Identity = 43/100 (43.00%), Postives = 59/100 (59.00%), Query Frame = 1 

Query: 26  VDPDNMTYEELVDLGETVGTQSRGLTQELIALLPVSRYRWGFFSRKK----SRNERCVIC 85
           +D DNM+YEEL+ LGE +G  S GL++E+I L  + +++    S          E C IC
Sbjct: 601 LDVDNMSYEELLALGERIGDVSTGLSEEVI-LKAMKQHKHTSSSPSSVELHQNIEPCCIC 660

Query: 86  QMEYKRGDKIITLPCKHKYHTSCGTKWLSIKKACPICYTE 122
           Q EY  GD + TL C H++H  C  +W+ IK  CPIC TE
Sbjct: 661 QEEYVEGDNLGTLKCGHEFHKDCIKQWVMIKNLCPICKTE 699

[Close]
BLAST of Cucsa.065490 vs. Swiss-Prot
Match: HIP1_ORYSJ (Probable E3 ubiquitin-protein ligase HIP1 OS=Oryza sativa subsp. japonica GN=HIP1 PE=1 SV=2)

HSP 1 Score: 80.1 bits (196), Expect = 2.0e-14
Identity = 39/93 (41.94%), Postives = 51/93 (54.84%), Query Frame = 1 

Query: 26  VDPDNMTYEELVDLGETVGTQSRGLTQELIALLPVSRYRWGFFSRKKSRNERCVICQMEY 85
           +D DNM+YEEL+ L E +G  S GL++E +  L   R    +        E C ICQ EY
Sbjct: 568 LDIDNMSYEELLALEERIGNVSTGLSEEEVTKLLKQRKFSSWRLEASVEEEPCCICQEEY 627

Query: 86  KRGDKIITLPCKHKYHTSCGTKWLSIKKACPIC 119
             GD + TL C H +H  C  +WL +K  CPIC
Sbjct: 628 VDGDDLGTLDCGHDFHVGCVRQWLVVKNTCPIC 660

[Close]
BLAST of Cucsa.065490 vs. TrEMBL
Match: A0A0A0L1Z5_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_4G290860 PE=4 SV=1)

HSP 1 Score: 279.3 bits (713), Expect = 2.5e-72
Identity = 129/129 (100.00%), Postives = 129/129 (100.00%), Query Frame = 1 

Query: 1   MSGNSMECPRRHSNYHEYQTIWQEIVDPDNMTYEELVDLGETVGTQSRGLTQELIALLPV 60
           MSGNSMECPRRHSNYHEYQTIWQEIVDPDNMTYEELVDLGETVGTQSRGLTQELIALLPV
Sbjct: 121 MSGNSMECPRRHSNYHEYQTIWQEIVDPDNMTYEELVDLGETVGTQSRGLTQELIALLPV 180

Query: 61  SRYRWGFFSRKKSRNERCVICQMEYKRGDKIITLPCKHKYHTSCGTKWLSIKKACPICYT 120
           SRYRWGFFSRKKSRNERCVICQMEYKRGDKIITLPCKHKYHTSCGTKWLSIKKACPICYT
Sbjct: 181 SRYRWGFFSRKKSRNERCVICQMEYKRGDKIITLPCKHKYHTSCGTKWLSIKKACPICYT 240

Query: 121 EVFGDTSKR 130
           EVFGDTSKR
Sbjct: 241 EVFGDTSKR 249

[Close]
BLAST of Cucsa.065490 vs. TrEMBL
Match: A0A0A0LX98_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_1G613470 PE=4 SV=1)

HSP 1 Score: 228.4 bits (581), Expect = 5.0e-57
Identity = 101/126 (80.16%), Postives = 114/126 (90.48%), Query Frame = 1 

Query: 4   NSMECPRRHSNYHEYQTIWQEIVDPDNMTYEELVDLGETVGTQSRGLTQELIALLPVSRY 63
           N +ECPRRH N H++Q +WQ+ VDPDNMTYEEL++LGETVGTQSRGL+QELIALLP+S+Y
Sbjct: 123 NHVECPRRHHNSHDFQVVWQDNVDPDNMTYEELLELGETVGTQSRGLSQELIALLPISKY 182

Query: 64  RWGFFSRKKSRNERCVICQMEYKRGDKIITLPCKHKYHTSCGTKWLSIKKACPICYTEVF 123
           ++ FFSRKKSR ERCVICQMEYKRGD+ ITLPCKH YH  CGTKWLSI KACPICYTEVF
Sbjct: 183 KYSFFSRKKSRGERCVICQMEYKRGDRRITLPCKHIYHAGCGTKWLSINKACPICYTEVF 242

Query: 124 GDTSKR 130
           GDTSKR
Sbjct: 243 GDTSKR 248

[Close]
BLAST of Cucsa.065490 vs. TrEMBL
Match: A0A067F1T9_CITSI (Uncharacterized protein OS=Citrus sinensis GN=CISIN_1g025212mg PE=4 SV=1)

HSP 1 Score: 219.2 bits (557), Expect = 3.0e-54
Identity = 94/123 (76.42%), Postives = 112/123 (91.06%), Query Frame = 1 

Query: 6   MECPRRHSNYHEYQTIWQEIVDPDNMTYEELVDLGETVGTQSRGLTQELIALLPVSRYRW 65
           +ECPRRH N HE+Q +WQ+ +DPD MTYEEL++LGETVGTQSRGL+QELI+LLP+S+Y+ 
Sbjct: 125 IECPRRHQNTHEHQVVWQDNIDPDQMTYEELLELGETVGTQSRGLSQELISLLPISKYKC 184

Query: 66  GFFSRKKSRNERCVICQMEYKRGDKIITLPCKHKYHTSCGTKWLSIKKACPICYTEVFGD 125
           GFF+RK+SRNERCVICQMEYKRGD+ +TLPCKH YH  CGT+WLSI KACPICYTEVFGD
Sbjct: 185 GFFTRKRSRNERCVICQMEYKRGDRRMTLPCKHVYHAGCGTRWLSINKACPICYTEVFGD 244

Query: 126 TSK 129
           +SK
Sbjct: 245 SSK 247

[Close]
BLAST of Cucsa.065490 vs. TrEMBL
Match: A0A0D2TL58_GOSRA (Uncharacterized protein OS=Gossypium raimondii GN=B456_009G072700 PE=4 SV=1)

HSP 1 Score: 215.3 bits (547), Expect = 4.4e-53
Identity = 96/125 (76.80%), Postives = 110/125 (88.00%), Query Frame = 1 

Query: 4   NSMECPRRHSNYHEYQTIWQEIVDPDNMTYEELVDLGETVGTQSRGLTQELIALLPVSRY 63
           NS++CPRR  N H+YQ IWQ+ VDPDNMTYEEL++LGE+VGTQSRGLTQELI+LLPVS+Y
Sbjct: 125 NSVDCPRRQHNAHDYQVIWQDCVDPDNMTYEELLELGESVGTQSRGLTQELISLLPVSKY 184

Query: 64  RWGFFSRKKSRNERCVICQMEYKRGDKIITLPCKHKYHTSCGTKWLSIKKACPICYTEVF 123
           +   FSRKKSR ERCVICQMEYKRG++ ITLPCKH YH  CGT+WLSI KACPICYTEVF
Sbjct: 185 KCSLFSRKKSRKERCVICQMEYKRGERQITLPCKHVYHAGCGTRWLSINKACPICYTEVF 244

Query: 124 GDTSK 129
           G+ SK
Sbjct: 245 GNGSK 249

[Close]
BLAST of Cucsa.065490 vs. TrEMBL
Match: I1LQI9_SOYBN (Uncharacterized protein OS=Glycine max GN=GLYMA_12G057100 PE=4 SV=1)

HSP 1 Score: 214.2 bits (544), Expect = 9.7e-53
Identity = 99/126 (78.57%), Postives = 110/126 (87.30%), Query Frame = 1 

Query: 4   NSMECPRRH-SNYHEYQTIWQEIVDPDNMTYEELVDLGETVGTQSRGLTQELIALLPVSR 63
           NS+ECPRRH SN ++YQ IWQ+ +DPDNMTYEEL++LGE VGTQSRGLTQE I+ LPVS+
Sbjct: 121 NSIECPRRHHSNSNDYQVIWQDNIDPDNMTYEELLELGEAVGTQSRGLTQEQISSLPVSK 180

Query: 64  YRWGFFSRKKSRNERCVICQMEYKRGDKIITLPCKHKYHTSCGTKWLSIKKACPICYTEV 123
           Y+ GFF RKKSR+ERCVICQMEYKRGDK ITLPCKH YH SCG KWLSI KACPICYTEV
Sbjct: 181 YKCGFFLRKKSRDERCVICQMEYKRGDKRITLPCKHVYHASCGNKWLSINKACPICYTEV 240

Query: 124 FGDTSK 129
           F D SK
Sbjct: 241 FADKSK 246

[Close]
BLAST of Cucsa.065490 vs. TAIR10
Match: AT3G63530.1 (AT3G63530.1 RING/U-box superfamily protein)

HSP 1 Score: 149.8 bits (377), Expect = 1.1e-36
Identity = 66/121 (54.55%), Postives = 86/121 (71.07%), Query Frame = 1 

Query: 7   ECPRRHSNYHEYQTIWQEIVDPDNMTYEELVDLGETVGTQSRGLTQELIALLPVSRYRWG 66
           EC     +    Q  WQ+ +DPD MTYEELV+LGE VGT+SRGL+QELI  LP  +Y++G
Sbjct: 126 ECIANEHDPDSPQVSWQDDIDPDTMTYEELVELGEAVGTESRGLSQELIETLPTKKYKFG 185

Query: 67  FFSRKKSRNERCVICQMEYKRGDKIITLPCKHKYHTSCGTKWLSIKKACPICYTEVFGDT 126
               +K   ERCVICQ++YK G++ + LPCKH YH+ C +KWLSI K CP+C +EVFG+ 
Sbjct: 186 SIFSRKRAGERCVICQLKYKIGERQMNLPCKHVYHSECISKWLSINKVCPVCNSEVFGEP 245

Query: 127 S 128
           S
Sbjct: 246 S 246

[Close]
BLAST of Cucsa.065490 vs. TAIR10
Match: AT3G19910.1 (AT3G19910.1 RING/U-box superfamily protein)

HSP 1 Score: 117.1 bits (292), Expect = 8.2e-27
Identity = 54/112 (48.21%), Postives = 72/112 (64.29%), Query Frame = 1 

Query: 16  HEYQTIWQEIVDPDNMTYEELVDLGETVGTQSRGLTQELIALLPVSRYRWGFFSRKKSRN 75
           H  Q  W E+ DPD ++YEEL+ LG+ VGT+SRGL+ + IA LP  RY+ G    +   N
Sbjct: 229 HTSQDAWDEM-DPDELSYEELLALGDIVGTESRGLSADTIASLPSKRYKEG--DNQNGTN 288

Query: 76  ERCVICQMEYKRGDKIITLPCKHKYHTSCGTKWLSIKKACPICYTEVFGDTS 128
           E CVIC+++Y+  + +I LPCKH YH+ C   WL I K CP+C  EV   TS
Sbjct: 289 ESCVICRLDYEDDEDLILLPCKHSYHSECINNWLKINKVCPVCSAEVSTSTS 337

[Close]
BLAST of Cucsa.065490 vs. TAIR10
Match: AT5G52140.1 (AT5G52140.1 RING/U-box superfamily protein)

HSP 1 Score: 100.9 bits (250), Expect = 6.1e-22
Identity = 50/105 (47.62%), Postives = 65/105 (61.90%), Query Frame = 1 

Query: 27  DPDNMTYEELVDLGETVGTQSRGLTQELIALLPVSRY-----RWGFFSRKK---SRNERC 86
           DP+NMTYEEL +LG++VG   +GL+QE I+ L   +Y      W     KK   + + +C
Sbjct: 175 DPENMTYEELTELGDSVGDVGKGLSQERISRLRTHKYGTKTKSWYCLHMKKKFVADDSQC 234

Query: 87  VICQMEYKRGDKIITLPCKHKYHTSCGTKWLSIKKACPICYTEVF 124
            IC MEY +GDKI TLPCKH YH  C ++WL   K C IC  EV+
Sbjct: 235 SICLMEYAKGDKITTLPCKHIYHKDCISQWLKQNKVCCICKAEVY 279

[Close]
BLAST of Cucsa.065490 vs. TAIR10
Match: AT3G47180.1 (AT3G47180.1 RING/U-box superfamily protein)

HSP 1 Score: 100.9 bits (250), Expect = 6.1e-22
Identity = 51/120 (42.50%), Postives = 73/120 (60.83%), Query Frame = 1 

Query: 6   MECPRRHSNYHEYQTIWQEI--VDPDNMTYEELVDLGETVGTQSRGLTQ-ELIALLPVSR 65
           +E    +SN  E     +E   +DPD ++YEEL+ LG+ +G ++RGLT  E+   L  S 
Sbjct: 90  LEDQESNSNLEEEDDFLEEEDEIDPDQLSYEELIALGDFIGVENRGLTPIEISTCLNAST 149

Query: 66  YRWGFFSRKKSRNERCVICQMEYKRGDKIITL-PCKHKYHTSCGTKWLSIKKACPICYTE 122
           Y    FS  K+  +RCV+CQME++  + ++ L PC H YH+ C TKWL  KK CPIC +E
Sbjct: 150 Y---VFSHNKNEIDRCVVCQMEFEERESLVVLRPCDHPYHSECITKWLETKKICPICCSE 206

[Close]
BLAST of Cucsa.065490 vs. TAIR10
Match: AT5G24870.1 (AT5G24870.1 RING/U-box superfamily protein)

HSP 1 Score: 88.6 bits (218), Expect = 3.1e-18
Identity = 48/100 (48.00%), Postives = 62/100 (62.00%), Query Frame = 1 

Query: 26  VDPDNMTYEELVDLGETVGTQSRGLTQE-LIALLPVSRYRWGFFSR----KKSRNERCVI 85
           +D DNM+YEEL+ L E +GT S  L++E L+  L  S YR    S      K  + +C I
Sbjct: 411 LDIDNMSYEELLALEEKMGTVSTALSEEALLKSLKSSIYRPNDESDDICLNKDDDVKCSI 470

Query: 86  CQMEYKRGDKIITLPCKHKYHTSCGTKWLSIKKACPICYT 121
           CQ EY  GD++ TLPC+HKYH SC  +WL +K  CPIC T
Sbjct: 471 CQEEYVDGDEVGTLPCQHKYHVSCAQQWLRMKNWCPICKT 510

[Close]
BLAST of Cucsa.065490 vs. NCBI nr
Match: gi|449448944|ref|XP_004142225.1| (PREDICTED: E3 ubiquitin ligase BIG BROTHER isoform X1 [Cucumis sativus])

HSP 1 Score: 279.3 bits (713), Expect = 3.5e-72
Identity = 129/129 (100.00%), Postives = 129/129 (100.00%), Query Frame = 1 

Query: 1   MSGNSMECPRRHSNYHEYQTIWQEIVDPDNMTYEELVDLGETVGTQSRGLTQELIALLPV 60
           MSGNSMECPRRHSNYHEYQTIWQEIVDPDNMTYEELVDLGETVGTQSRGLTQELIALLPV
Sbjct: 113 MSGNSMECPRRHSNYHEYQTIWQEIVDPDNMTYEELVDLGETVGTQSRGLTQELIALLPV 172

Query: 61  SRYRWGFFSRKKSRNERCVICQMEYKRGDKIITLPCKHKYHTSCGTKWLSIKKACPICYT 120
           SRYRWGFFSRKKSRNERCVICQMEYKRGDKIITLPCKHKYHTSCGTKWLSIKKACPICYT
Sbjct: 173 SRYRWGFFSRKKSRNERCVICQMEYKRGDKIITLPCKHKYHTSCGTKWLSIKKACPICYT 232

Query: 121 EVFGDTSKR 130
           EVFGDTSKR
Sbjct: 233 EVFGDTSKR 241

[Close]
BLAST of Cucsa.065490 vs. NCBI nr
Match: gi|700199013|gb|KGN54171.1| (hypothetical protein Csa_4G290860 [Cucumis sativus])

HSP 1 Score: 279.3 bits (713), Expect = 3.5e-72
Identity = 129/129 (100.00%), Postives = 129/129 (100.00%), Query Frame = 1 

Query: 1   MSGNSMECPRRHSNYHEYQTIWQEIVDPDNMTYEELVDLGETVGTQSRGLTQELIALLPV 60
           MSGNSMECPRRHSNYHEYQTIWQEIVDPDNMTYEELVDLGETVGTQSRGLTQELIALLPV
Sbjct: 121 MSGNSMECPRRHSNYHEYQTIWQEIVDPDNMTYEELVDLGETVGTQSRGLTQELIALLPV 180

Query: 61  SRYRWGFFSRKKSRNERCVICQMEYKRGDKIITLPCKHKYHTSCGTKWLSIKKACPICYT 120
           SRYRWGFFSRKKSRNERCVICQMEYKRGDKIITLPCKHKYHTSCGTKWLSIKKACPICYT
Sbjct: 181 SRYRWGFFSRKKSRNERCVICQMEYKRGDKIITLPCKHKYHTSCGTKWLSIKKACPICYT 240

Query: 121 EVFGDTSKR 130
           EVFGDTSKR
Sbjct: 241 EVFGDTSKR 249

[Close]
BLAST of Cucsa.065490 vs. NCBI nr
Match: gi|659097625|ref|XP_008449725.1| (PREDICTED: E3 ubiquitin ligase BIG BROTHER-like isoform X1 [Cucumis melo])

HSP 1 Score: 274.6 bits (701), Expect = 8.7e-71
Identity = 126/129 (97.67%), Postives = 128/129 (99.22%), Query Frame = 1 

Query: 1   MSGNSMECPRRHSNYHEYQTIWQEIVDPDNMTYEELVDLGETVGTQSRGLTQELIALLPV 60
           MSGNSMECPRRHSNYHEYQTIWQ+IVDPDNMTYEELVDLGETVGTQSRGLTQELIALLPV
Sbjct: 114 MSGNSMECPRRHSNYHEYQTIWQDIVDPDNMTYEELVDLGETVGTQSRGLTQELIALLPV 173

Query: 61  SRYRWGFFSRKKSRNERCVICQMEYKRGDKIITLPCKHKYHTSCGTKWLSIKKACPICYT 120
           S YRWGFFSRKKSRNERCVICQMEYKRGDKIITLPCKH+YHTSCGTKWLSIKKACPICYT
Sbjct: 174 SSYRWGFFSRKKSRNERCVICQMEYKRGDKIITLPCKHRYHTSCGTKWLSIKKACPICYT 233

Query: 121 EVFGDTSKR 130
           EVFGDTSKR
Sbjct: 234 EVFGDTSKR 242

[Close]
BLAST of Cucsa.065490 vs. NCBI nr
Match: gi|778693179|ref|XP_011653591.1| (PREDICTED: E3 ubiquitin ligase BIG BROTHER isoform X2 [Cucumis sativus])

HSP 1 Score: 263.5 bits (672), Expect = 2.0e-67
Identity = 122/125 (97.60%), Postives = 123/125 (98.40%), Query Frame = 1 

Query: 5   SMECPRRHSNYHEYQTIWQEIVDPDNMTYEELVDLGETVGTQSRGLTQELIALLPVSRYR 64
           +M  PRRHSNYHEYQTIWQEIVDPDNMTYEELVDLGETVGTQSRGLTQELIALLPVSRYR
Sbjct: 112 NMSGPRRHSNYHEYQTIWQEIVDPDNMTYEELVDLGETVGTQSRGLTQELIALLPVSRYR 171

Query: 65  WGFFSRKKSRNERCVICQMEYKRGDKIITLPCKHKYHTSCGTKWLSIKKACPICYTEVFG 124
           WGFFSRKKSRNERCVICQMEYKRGDKIITLPCKHKYHTSCGTKWLSIKKACPICYTEVFG
Sbjct: 172 WGFFSRKKSRNERCVICQMEYKRGDKIITLPCKHKYHTSCGTKWLSIKKACPICYTEVFG 231

Query: 125 DTSKR 130
           DTSKR
Sbjct: 232 DTSKR 236

[Close]
BLAST of Cucsa.065490 vs. NCBI nr
Match: gi|659097627|ref|XP_008449726.1| (PREDICTED: E3 ubiquitin ligase BIG BROTHER-like isoform X2 [Cucumis melo])

HSP 1 Score: 258.8 bits (660), Expect = 4.9e-66
Identity = 119/125 (95.20%), Postives = 122/125 (97.60%), Query Frame = 1 

Query: 5   SMECPRRHSNYHEYQTIWQEIVDPDNMTYEELVDLGETVGTQSRGLTQELIALLPVSRYR 64
           +M  PRRHSNYHEYQTIWQ+IVDPDNMTYEELVDLGETVGTQSRGLTQELIALLPVS YR
Sbjct: 113 NMSGPRRHSNYHEYQTIWQDIVDPDNMTYEELVDLGETVGTQSRGLTQELIALLPVSSYR 172

Query: 65  WGFFSRKKSRNERCVICQMEYKRGDKIITLPCKHKYHTSCGTKWLSIKKACPICYTEVFG 124
           WGFFSRKKSRNERCVICQMEYKRGDKIITLPCKH+YHTSCGTKWLSIKKACPICYTEVFG
Sbjct: 173 WGFFSRKKSRNERCVICQMEYKRGDKIITLPCKHRYHTSCGTKWLSIKKACPICYTEVFG 232

Query: 125 DTSKR 130
           DTSKR
Sbjct: 233 DTSKR 237

The following BLAST results are available for this feature:
BLAST of Cucsa.065490 vs. Swiss-Prot
Match NameE-valueIdentityDescription
BB_ARATH2.0e-3554.55E3 ubiquitin ligase BIG BROTHER OS=Arabidopsis thaliana GN=BB PE=1 SV=1[more]
BBR_ARATH1.5e-2548.21E3 ubiquitin ligase BIG BROTHER-related OS=Arabidopsis thaliana GN=BBR PE=2 SV=1[more]
MBR2_ARATH2.1e-1642.11E3 ubiquitin-protein ligase MBR2 OS=Arabidopsis thaliana GN=MBR2 PE=1 SV=1[more]
MBR1_ARATH4.0e-1543.00E3 ubiquitin-protein ligase MBR1 OS=Arabidopsis thaliana GN=MBR1 PE=1 SV=1[more]
HIP1_ORYSJ2.0e-1441.94Probable E3 ubiquitin-protein ligase HIP1 OS=Oryza sativa subsp. japonica GN=HIP... [more]
BLAST of Cucsa.065490 vs. TrEMBL
Match NameE-valueIdentityDescription
A0A0A0L1Z5_CUCSA2.5e-72100.00Uncharacterized protein OS=Cucumis sativus GN=Csa_4G290860 PE=4 SV=1[more]
A0A0A0LX98_CUCSA5.0e-5780.16Uncharacterized protein OS=Cucumis sativus GN=Csa_1G613470 PE=4 SV=1[more]
A0A067F1T9_CITSI3.0e-5476.42Uncharacterized protein OS=Citrus sinensis GN=CISIN_1g025212mg PE=4 SV=1[more]
A0A0D2TL58_GOSRA4.4e-5376.80Uncharacterized protein OS=Gossypium raimondii GN=B456_009G072700 PE=4 SV=1[more]
I1LQI9_SOYBN9.7e-5378.57Uncharacterized protein OS=Glycine max GN=GLYMA_12G057100 PE=4 SV=1[more]
BLAST of Cucsa.065490 vs. TAIR10
Match NameE-valueIdentityDescription
AT3G63530.11.1e-3654.55 RING/U-box superfamily protein[more]
AT3G19910.18.2e-2748.21 RING/U-box superfamily protein[more]
AT5G52140.16.1e-2247.62 RING/U-box superfamily protein[more]
AT3G47180.16.1e-2242.50 RING/U-box superfamily protein[more]
AT5G24870.13.1e-1848.00 RING/U-box superfamily protein[more]
BLAST of Cucsa.065490 vs. NCBI nr
Match NameE-valueIdentityDescription
gi|449448944|ref|XP_004142225.1|3.5e-72100.00PREDICTED: E3 ubiquitin ligase BIG BROTHER isoform X1 [Cucumis sativus][more]
gi|700199013|gb|KGN54171.1|3.5e-72100.00hypothetical protein Csa_4G290860 [Cucumis sativus][more]
gi|659097625|ref|XP_008449725.1|8.7e-7197.67PREDICTED: E3 ubiquitin ligase BIG BROTHER-like isoform X1 [Cucumis melo][more]
gi|778693179|ref|XP_011653591.1|2.0e-6797.60PREDICTED: E3 ubiquitin ligase BIG BROTHER isoform X2 [Cucumis sativus][more]
gi|659097627|ref|XP_008449726.1|4.9e-6695.20PREDICTED: E3 ubiquitin ligase BIG BROTHER-like isoform X2 [Cucumis melo][more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR001841Znf_RING
IPR013083Znf_RING/FYVE/PHD
Vocabulary: Molecular Function
TermDefinition
GO:0004842ubiquitin-protein transferase activity
GO:0005515protein binding
GO:0008270zinc ion binding
Vocabulary: Biological Process
TermDefinition
GO:0016567protein ubiquitination
GO:0046621negative regulation of organ growth
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0046621 negative regulation of organ growth
biological_process GO:0016567 protein ubiquitination
biological_process GO:0008150 biological_process
cellular_component GO:0005575 cellular_component
molecular_function GO:0016874 ligase activity
molecular_function GO:0005515 protein binding
molecular_function GO:0004842 ubiquitin-protein transferase activity
molecular_function GO:0008270 zinc ion binding
molecular_function GO:0003674 molecular_function
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cucsa.065490.1Cucsa.065490.1mRNA


InterPro
Analysis Name: InterPro Annotations of cucumber (Gy14)
Date Performed: 2017-01-17
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001841Zinc finger, RING-typePFAMPF13639zf-RING_2coord: 77..118
score: 2.0
IPR001841Zinc finger, RING-typeSMARTSM00184ring_2coord: 78..118
score: 2.
IPR001841Zinc finger, RING-typePROFILEPS50089ZF_RING_2coord: 78..118
score: 11
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3DG3DSA:3.30.40.10coord: 74..122
score: 7.5
NoneNo IPR availablePANTHERPTHR14155RING FINGER DOMAIN-CONTAININGcoord: 9..125
score: 1.6
NoneNo IPR availableunknownSSF57850RING/U-boxcoord: 77..124
score: 1.45

Synteny
The following gene(s) are orthologous to this gene:
GeneOrthologueOrganismBlock
Cucsa.065490Cla008540Watermelon (97103) v1cgywmB065
Cucsa.065490Cla015236Watermelon (97103) v1cgywmB060
Cucsa.065490Csa4G290860Cucumber (Chinese Long) v2cgycuB055
Cucsa.065490MELO3C023546Melon (DHL92) v3.5.1cgymeB061
Cucsa.065490MELO3C014603Melon (DHL92) v3.5.1cgymeB063
Cucsa.065490ClCG02G023330Watermelon (Charleston Gray)cgywcgB062
Cucsa.065490CSPI07G19300Wild cucumber (PI 183967)cgycpiB061
Cucsa.065490CSPI04G13830Wild cucumber (PI 183967)cgycpiB055
Cucsa.065490CmaCh20G000770Cucurbita maxima (Rimu)cgycmaB0101
Cucsa.065490Lsi10G010550Bottle gourd (USVL1VR-Ls)cgylsiB057
Cucsa.065490Lsi02G025800Bottle gourd (USVL1VR-Ls)cgylsiB062
Cucsa.065490Cp4.1LG05g05080Cucurbita pepo (Zucchini)cgycpeB0102
Cucsa.065490MELO3C014603.2Melon (DHL92) v3.6.1cgymedB062
Cucsa.065490MELO3C023546.2Melon (DHL92) v3.6.1cgymedB059
Cucsa.065490CsaV3_4G024180Cucumber (Chinese Long) v3cgycucB057
Cucsa.065490CsaV3_7G031110Cucumber (Chinese Long) v3cgycucB063
Cucsa.065490Cla97C02G049410Watermelon (97103) v2cgywmbB059
Cucsa.065490Bhi10G000248Wax gourdcgywgoB066
Cucsa.065490Bhi09G000526Wax gourdcgywgoB070
Cucsa.065490Carg02717Silver-seed gourdcarcgyB1034
The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:
GeneOrganismBlock
Cucsa.065490Silver-seed gourdcarcgyB0594
Cucsa.065490Cucurbita maxima (Rimu)cgycmaB0103
Cucsa.065490Cucurbita moschata (Rifu)cgycmoB0096
Cucsa.065490Cucurbita moschata (Rifu)cgycmoB0098
Cucsa.065490Cucurbita pepo (Zucchini)cgycpeB0097