Cucsa.038070 (gene) Cucumber (Gy14) v1 | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Cucsa.038070
Type	gene
Organism	Cucumis sativus (Cucumber (Gy14) v1)
Description	Pectin lyase-like superfamily protein
Location	scaffold00493 : 119024 .. 120702 (-)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCCAACCCAACAAAAAGTTTAGTCTTCGTTCTCTTCTTCCATTTGTTCATTCAAATTTCAAGTGCAGGCAACTACAATGTGATAACATTCGGTGCAAAACCGGATGGAAAAACTGACTCAACCCAATCATTTCTGAAAGCGTGGATGTCTGCATGTAGCTCCTTGACACGATCCACCATCAACGTCCCTAAGGGAAGGTTCTTGCTTACATCCATCACATTTAGAGGTCCATGTAAGAACAGCAACATTACATTTCAATTGAATGGAACACTCGTGGCACCTCTTGACTATAATGCTCTTGGAGATTCTAGGTATTGGATTTTGTTCACCAAAGTTAATGGAATTTCTTTCATCGGTGGCAATATTGATGGAAAAGGCACCGACTATTGGGCTTGCAAGGGCTCTGGAAAAAAATGTCCCCCGGGAGCAAGGGTATGCGTGTTAATTACTCAATACTTTATTGAATTCCAAAACTGCACATCGACTATCTTCATTAGAAAGCAGAAAAAAAACACCAAAAATATTACTTTTTACCCAAAAATTGACCAACATACTAACTTTACAATTGAGAGCTTGTTTAGATATTAATTTGAGAAAAAAAGTGCTTTCCAAACGTCTTATTTTTATTTAAACGAAAGGGGTTTAAATGCATTTTAAAAGTTATTTTGTAGTAGACATGGGACATTTTGATCTTTTTAAAATTTCATTTTTTAAATTAAAACTTGAAAATGTATTATTCCAAACAACACACCCTGAGTAAGCTACTATATATCCAAAAGCATCCAAGTGTATTTTTTAAAATTTATTGTGACATGTTTATTTATATATATATGTATAGTAACACATGTTTTCTTTTGTTTTAACAAATGAAATACAATCAATAACATTCAATTCGGCAAGCAACGTCATAATTAGTGGACTAAGATCAATCAACACTCGACAAACTCATATTGTGATCAATAGTTGCAACAATGTGATGGTCAAGAATGTGAAGATAATGGCTCCAGACCAAGGCCCCAATACTGATGGCATCCATGTGCAATCCTCCACTGCTGTAACCATCTCTTGAACTACCATTAAAACGGGAGATGATTGCATTTCTATTGGTGATGGAACAAATAATTTGTTTATGAGTGATATTAAATGTGGGCCCGCCATGGTGTAAGGTAACAAATCTAATATATGCACACACTAAAATGATGGCCGGTAATCTTTTTTTTTTTGAGTTAATAAAATAATATGATAATAATTTCAATTAATGCAGTATCGGGAGCCTAGGAAAAGAGGTCAATGAAAATGGAGTTGAAAATATAAGATTGATGAATACAGTGTTTACAAAATCAGATAATGGTGTGAGGATAAAGACATCGCCTACGTCTAGCAATGGTTTCGTAAAACATGTTGTGTTTGAAAATATAACTATGCATAATGTTAAACATCCAATCTTAATCGATCAAAATTATTGTCCCAATAATCAAGGTTGTTCTGTCGAGGTAATTATTATTAAATTAATTAAATTGTTGGCATACATATACTTAATTACCTCTCATGTTTGCTAATATTTATTTTTAATTGTTTTCATCGTTGTTTTAAAGGATTCTGGAGTGAAGATAAGTGATGTAACATACAAAAATATTGAAGGAACGTCAGCAACGGCAGAAGAAGAAGGAGAATAA

mRNA sequence

ATGGCCAACCCAACAAAAAGTTTAGTCTTCGTTCTCTTCTTCCATTTGTTCATTCAAATTTCAAGTGCAGGCAACTACAATGTGATAACATTCGGTGCAAAACCGGATGGAAAAACTGACTCAACCCAATCATTTCTGAAAGCGTGGATGTCTGCATGTAGCTCCTTGACACGATCCACCATCAACGTCCCTAAGGGAAGGTTCTTGCTTACATCCATCACATTTAGAGGTCCATGTAAGAACAGCAACATTACATTTCAATTGAATGGAACACTCGTGGCACCTCTTGACTATAATGCTCTTGGAGATTCTAGGTATTGGATTTTGTTCACCAAAGTTAATGGAATTTCTTTCATCGGTGGCAATATTGATGGAAAAGGCACCGACTATTGGGCTTGCAAGGGCTCTGGAAAAAAATGTCCCCCGGGAGCAAGGTtgcaacaatgtgatggtcaagaatatgattgcatttctattggtgatggaacaaataatttgtttatgagtgatattaaatgtgggcccgccatggtTATCGGGAGCCTAGGAAAAGAGGTCAATGAAAATGGAGTTGAAAATATAAGATTGATGAATACAGTGTTTACAAAATCAGATAATGGTGTGAGGATAAAGACATCGCCTACGTCTAGCAATGGTTTCGTAAAACATGTTGTGTTTGAAAATATAACTATGCATAATGTTAAACATCCAATCTTAATCGATCAAAATTATTGTCCCAATAATCAAGGTTGTTCTGTCGAGGATTCTGGAGTGAAGATAAGTGATGTAACATACAAAAATATTGAAGGAACGTCAGCAACGGCAGAAGAAGAAGGAGAATaa

Coding sequence (CDS)

ATGGCCAACCCAACAAAAAGTTTAGTCTTCGTTCTCTTCTTCCATTTGTTCATTCAAATTTCAAGTGCAGGCAACTACAATGTGATAACATTCGGTGCAAAACCGGATGGAAAAACTGACTCAACCCAATCATTTCTGAAAGCGTGGATGTCTGCATGTAGCTCCTTGACACGATCCACCATCAACGTCCCTAAGGGAAGGTTCTTGCTTACATCCATCACATTTAGAGGTCCATGTAAGAACAGCAACATTACATTTCAATTGAATGGAACACTCGTGGCACCTCTTGACTATAATGCTCTTGGAGATTCTAGGTATTGGATTTTGTTCACCAAAGTTAATGGAATTTCTTTCATCGGTGGCAATATTGATGGAAAAGGCACCGACTATTGGGCTTGCAAGGGCTCTGGAAAAAAATGTCCCCCGGGAGCAAGGTTGCAACAATGTGATGGTCAAGAATATGATTGCATTTCTATTGGTGATGGAACAAATAATTTGTTTATGAGTGATATTAAATGTGGGCCCGCCATGGTTATCGGGAGCCTAGGAAAAGAGGTCAATGAAAATGGAGTTGAAAATATAAGATTGATGAATACAGTGTTTACAAAATCAGATAATGGTGTGAGGATAAAGACATCGCCTACGTCTAGCAATGGTTTCGTAAAACATGTTGTGTTTGAAAATATAACTATGCATAATGTTAAACATCCAATCTTAATCGATCAAAATTATTGTCCCAATAATCAAGGTTGTTCTGTCGAGGATTCTGGAGTGAAGATAAGTGATGTAACATACAAAAATATTGAAGGAACGTCAGCAACGGCAGAAGAAGAAGGAGAATAA

Protein sequence

MANPTKSLVFVLFFHLFIQISSAGNYNVITFGAKPDGKTDSTQSFLKAWMSACSSLTRSTINVPKGRFLLTSITFRGPCKNSNITFQLNGTLVAPLDYNALGDSRYWILFTKVNGISFIGGNIDGKGTDYWACKGSGKKCPPGARLQQCDGQEYDCISIGDGTNNLFMSDIKCGPAMVIGSLGKEVNENGVENIRLMNTVFTKSDNGVRIKTSPTSSNGFVKHVVFENITMHNVKHPILIDQNYCPNNQGCSVEDSGVKISDVTYKNIEGTSATAEEEGE*

Homology

[Close]

BLAST of Cucsa.038070 vs. Swiss-Prot
Match: PGLR_PRUPE (Polygalacturonase OS=Prunus persica PE=2 SV=1)

HSP 1 Score: 147.1 bits (370), Expect = 2.8e-34
Identity = 72/139 (51.80%), Postives = 92/139 (66.19%), Query Frame = 1


Query: 7   SLVFVLFFHLFIQISSAGNYNVITFGAKPDGKTDSTQSFLKAWMSACSSLTRSTINVPKG 66
           SL+FV  F +   I+S   YNV + GAK DGKTDST++FL AW  AC+S+    I VP G
Sbjct: 11  SLIFV--FMINSAIASPLTYNVASLGAKADGKTDSTKAFLSAWAKACASMNPGVIYVPAG 70

Query: 67  RFLLTSITFRGPCKNSNITFQLNGTLVAPLDYNALGDSRYWILFTKVNGISFIGGNIDGK 126
            F L  + F GPCKN+ ITF++ GTLVAP DY  +G++  WI F  VNG++  GG +DG+
Sbjct: 71  TFFLRDVVFSGPCKNNAITFRIAGTLVAPSDYRVIGNAANWIFFHHVNGVTISGGILDGQ 130

Query: 127 GTDYWACKG-SGKKCPPGA 145
           GT  WACK   G+ CP GA
Sbjct: 131 GTALWACKACHGESCPSGA 147

HSP 2 Score: 135.6 bits (340), Expect = 8.5e-31
Identity = 63/122 (51.64%), Postives = 92/122 (75.41%), Query Frame = 1


Query: 155 DCISIGDGTNNLFMSDIKCGPA--MVIGSLGKEVNENGVENIRLMNTVFTKSDNGVRIKT 214
           DC+SIG GT+NL++  + CGP   + IGSLGKE  E GV+N+ +    F+ + NG+RIK+
Sbjct: 220 DCVSIGPGTSNLWIEGVACGPGHGISIGSLGKEQEEAGVQNVTVKTVTFSGTQNGLRIKS 279

Query: 215 SPTSSNGFVKHVVFENITMHNVKHPILIDQNYCPNNQGCSVEDSGVKISDVTYKNIEGTS 274
               S GF ++++F++ TM NV++PI+IDQ+YCP+N+GC  + SGV+ISDVTY++I GTS
Sbjct: 280 WGRPSTGFARNILFQHATMVNVENPIVIDQHYCPDNKGCPGQVSGVQISDVTYEDIHGTS 339

[Close]

BLAST of Cucsa.038070 vs. Swiss-Prot
Match: PGLR_GOSBA (Polygalacturonase OS=Gossypium barbadense GN=G9 PE=2 SV=1)

HSP 1 Score: 129.0 bits (323), Expect = 8.0e-29
Identity = 66/124 (53.23%), Postives = 83/124 (66.94%), Query Frame = 1


Query: 155 DCISIGDGTNNLFMSDIKCGPA--MVIGSLGKEVNENGVENIRLMNTVFTKSDNGVRIKT 214
           DCISIGDGT N+ + +I CGP   + IGSLGK  NE  VE I++ N   T + NG RIKT
Sbjct: 222 DCISIGDGTKNMVIKEITCGPGHGISIGSLGKFQNEEPVEGIKISNCTITNTSNGARIKT 281

Query: 215 SPTSSNGFVKHVVFENITMHNVKHPILIDQNYCPNNQGCSVEDSGVKISDVTYKNIEGTS 274
            P    G V  + FE+ITM+NV  PILIDQ YCP N+    E+S VK+S++++KNI GTS
Sbjct: 282 WPGEHGGAVSEIHFEDITMNNVSSPILIDQQYCPWNKCKKNEESKVKLSNISFKNIRGTS 341

Query: 275 ATAE 277
           A  E
Sbjct: 342 ALPE 345

HSP 2 Score: 89.7 bits (221), Expect = 5.4e-17
Identity = 53/124 (42.74%), Postives = 73/124 (58.87%), Query Frame = 1


Query: 9   VFVLFFHLFIQISSA--GNYNVIT-FGAKPDGKTDSTQSFLKAWMSACSSLTRSTINVPK 68
           +FVL   LFI  S      ++V+  FGAK DGKTD ++ FL AW  AC+S+T ST+ +PK
Sbjct: 11  MFVLLL-LFISASKVQPDAFDVVAKFGAKADGKTDLSKPFLDAWKEACASVTPSTVVIPK 70

Query: 69  GRFLLTSITFRGPCKNSNITFQLNGTLVAPLDYNALGDSRYWILFTKVNGISFIGGNI-D 128
           G +LL+ +   GPCK + I   + GT+ AP D +A  D   W+ F  V      GG I D
Sbjct: 71  GTYLLSKVNLEGPCK-APIEINVQGTIQAPADPSAFKDPN-WVRFYSVENFKMFGGGIFD 130

[Close]

BLAST of Cucsa.038070 vs. Swiss-Prot
Match: PGLR_GOSHI (Polygalacturonase OS=Gossypium hirsutum GN=G9 PE=2 SV=1)

HSP 1 Score: 129.0 bits (323), Expect = 8.0e-29
Identity = 66/124 (53.23%), Postives = 83/124 (66.94%), Query Frame = 1


Query: 155 DCISIGDGTNNLFMSDIKCGPA--MVIGSLGKEVNENGVENIRLMNTVFTKSDNGVRIKT 214
           DCISIGDGT N+ + +I CGP   + IGSLGK  NE  VE I++ N   T + NG RIKT
Sbjct: 222 DCISIGDGTKNMVIKEITCGPGHGISIGSLGKFQNEEPVEGIKISNCTITNTSNGARIKT 281

Query: 215 SPTSSNGFVKHVVFENITMHNVKHPILIDQNYCPNNQGCSVEDSGVKISDVTYKNIEGTS 274
            P    G V  + FE+ITM+NV  PILIDQ YCP N+    E+S VK+S++++KNI GTS
Sbjct: 282 WPGEHGGAVSEIHFEDITMNNVSSPILIDQQYCPWNKCKKNEESKVKLSNISFKNIRGTS 341

Query: 275 ATAE 277
           A  E
Sbjct: 342 ALPE 345

HSP 2 Score: 90.5 bits (223), Expect = 3.1e-17
Identity = 50/121 (41.32%), Postives = 69/121 (57.02%), Query Frame = 1


Query: 9   VFVLFFHLFIQISSAGNYNVITFGAKPDGKTDSTQSFLKAWMSACSSLTRSTINVPKGRF 68
           V +L F    ++ S     V  FGAK DGKTD ++ FL AW  AC+S+T ST+ +PKG +
Sbjct: 13  VLLLLFISASKVQSDAFDVVAKFGAKADGKTDLSKPFLDAWKEACASVTPSTVVIPKGTY 72

Query: 69  LLTSITFRGPCKNSNITFQLNGTLVAPLDYNALGDSRYWILFTKVNGISFIGGNI-DGKG 128
           LL+ +   GPCK + I   + GT+ AP D +A  D   W+ F  V      GG I DG+G
Sbjct: 73  LLSKVNLEGPCK-APIEINVQGTIQAPADPSAFKDPN-WVRFYSVENFKMFGGGIFDGQG 131

[Close]

BLAST of Cucsa.038070 vs. Swiss-Prot
Match: PGLR6_ARATH (Probable polygalacturonase At2g43860 OS=Arabidopsis thaliana GN=At2g43860 PE=2 SV=1)

HSP 1 Score: 122.5 bits (306), Expect = 7.5e-27
Identity = 72/217 (33.18%), Postives = 116/217 (53.46%), Query Frame = 1


Query: 27  NVITFGAKPDGKTDSTQSFLKAWMSACSSLTRSTINVPKGRFLLTSITFRG-PCKNSNIT 86
           NV+++GAKPDG  DST++FL AW  AC+S   +TI VPKGRFL+ ++ F G  CK + I+
Sbjct: 34  NVLSYGAKPDGSKDSTKAFLAAWDVACASANPTTIIVPKGRFLVGNLVFHGNECKQAPIS 93

Query: 87  FQLNGTLVAPLDYNALGDSRYWILFTKVNGISFIGGNIDGKGTDYWACKGS-GKKCPPGA 146
            ++ G++VAP D+  +  S++WI F  V  +S  GG +D +GT  W CK + G  CP GA
Sbjct: 94  IRIAGSIVAPEDFRIIASSKHWIWFEDVTDVSIYGGILDAQGTSLWKCKNNGGHNCPTGA 153

Query: 147 RLQQCDGQEYDCIS------------IGDGTNNLFMSDIKCGPAMVIGSLGKEVNENGV- 206
           +     G     IS            + D +NN+ +  +K      + +     N +G+ 
Sbjct: 154 KSLVFSGSNNIKISGLTSINSQKFHIVIDNSNNVNIDGVK------VSADENSPNTDGIH 213

Query: 207 ----ENIRLMNTVFTKSDNGVRIKTSPTSSNGFVKHV 225
                ++ + N+     D+ + I   P S+N F++ +
Sbjct: 214 VESSHSVHITNSRIGTGDDCISI--GPGSTNVFIQTI 242

HSP 2 Score: 121.3 bits (303), Expect = 1.7e-26
Identity = 60/122 (49.18%), Postives = 87/122 (71.31%), Query Frame = 1


Query: 155 DCISIGDGTNNLFMSDIKCGPA--MVIGSLGKEVNENGVENIRLMNTVFTKSDNGVRIKT 214
           DCISIG G+ N+F+  I+CGP   + IGSLG+   E GV+N+ + N  F  ++NGVRIKT
Sbjct: 226 DCISIGPGSTNVFIQTIRCGPGHGISIGSLGRAEEEQGVDNVTVSNVDFMGTNNGVRIKT 285

Query: 215 SPTSSNGFVKHVVFENITMHNVKHPILIDQNYCPNNQGCSVEDSGVKISDVTYKNIEGTS 274
               SN F +++VF++I M  VK+PI+IDQ+YC  ++ C  ++SGVK+S+V Y++I GTS
Sbjct: 286 WGKDSNSFARNIVFQHINMKMVKNPIIIDQHYC-LHKPCPKQESGVKVSNVRYEDIHGTS 345

[Close]

BLAST of Cucsa.038070 vs. Swiss-Prot
Match: PGLR2_ARATH (Exopolygalacturonase clone GBGA483 OS=Arabidopsis thaliana GN=At3g07850 PE=2 SV=2)

HSP 1 Score: 121.7 bits (304), Expect = 1.3e-26
Identity = 64/134 (47.76%), Postives = 87/134 (64.93%), Query Frame = 1


Query: 143 GARLQQCDGQEYDCISIGDGTNNLFMSDIKCGPA--MVIGSLGKEVNENGVENIRLMNTV 202
           GA+++  D    DC+SIGDGT NL + +++CGP   + IGSLG+  NE  V+ + +   +
Sbjct: 254 GAKIKTGD----DCVSIGDGTENLIVENVECGPGHGISIGSLGRYPNEQPVKGVTVRKCL 313

Query: 203 FTKSDNGVRIKTSPTSSNGFVKHVVFENITMHNVKHPILIDQNYCPNNQGCSVEDSGVKI 262
              +DNGVRIKT P S  G   +++FE+ITM NV  P+LIDQ YCP     +   S VK+
Sbjct: 314 IKNTDNGVRIKTWPGSPPGIASNILFEDITMDNVSLPVLIDQEYCPYGHCKAGVPSQVKL 373

Query: 263 SDVTYKNIEGTSAT 275
           SDVT K I+GTSAT
Sbjct: 374 SDVTIKGIKGTSAT 383

HSP 2 Score: 73.6 bits (179), Expect = 4.0e-12
Identity = 42/112 (37.50%), Postives = 58/112 (51.79%), Query Frame = 1


Query: 32  GAKPDGKTDSTQSFLKAWMSACSSLTRSTINVPKGRFLLTSITFRGPCKNSNITFQLNGT 91
           GAK D KTD + +F  AW  AC++   STI VPKG +++ S+ F+GPCK   +T +LNG 
Sbjct: 76  GAKGDSKTDDSAAFAAAWKEACAA--GSTITVPKGEYMVESLEFKGPCKGP-VTLELNGN 135

Query: 92  LVAPLDYNALGDSRYWILFTKVNGISFIGGN--IDGKGTDYWA---CKGSGK 139
             AP           WI F  +   +  G     DG+G+  W    C  +GK
Sbjct: 136 FKAPATVKTTKPHAGWIDFENIADFTLNGNKAIFDGQGSLAWKANDCAKTGK 184

[Close]

BLAST of Cucsa.038070 vs. TrEMBL
Match: A0A0A0KZM5_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_4G153300 PE=3 SV=1)

HSP 1 Score: 305.4 bits (781), Expect = 6.9e-80
Identity = 147/154 (95.45%), Postives = 149/154 (96.75%), Query Frame = 1


Query: 1   MANPTKSLVFVLFFHLFIQISSAGNYNVITFGAKPDGKTDSTQSFLKAWMSACSSLTRST 60
           MANPTKSLVFVLFFHLFIQISSAGNYNVITFGAKPDGKTDSTQSFLKAWMSACSSLTRST
Sbjct: 1   MANPTKSLVFVLFFHLFIQISSAGNYNVITFGAKPDGKTDSTQSFLKAWMSACSSLTRST 60

Query: 61  INVPKGRFLLTSITFRGPCKNSNITFQLNGTLVAPLDYNALGDSRYWILFTKVNGISFIG 120
           INVPKGRFLLTSITFRGPCKNSNITFQLNGTLVAPLDYNALGDSRYWILFTKVNGISFIG
Sbjct: 61  INVPKGRFLLTSITFRGPCKNSNITFQLNGTLVAPLDYNALGDSRYWILFTKVNGISFIG 120

Query: 121 GNIDGKGTDYWACKGSGKKCPPGARLQQCDGQEY 155
           GNIDGKGTDYWACKGSGKKCPPGAR  +   Q+Y
Sbjct: 121 GNIDGKGTDYWACKGSGKKCPPGARKAEKKHQKY 154

[Close]

BLAST of Cucsa.038070 vs. TrEMBL
Match: M1A9G5_SOLTU (Uncharacterized protein OS=Solanum tuberosum GN=PGSC0003DMG403006909 PE=3 SV=1)

HSP 1 Score: 266.5 bits (680), Expect = 3.6e-68
Identity = 137/274 (50.00%), Postives = 184/274 (67.15%), Query Frame = 1


Query: 14  FHLFI---QISSAGNYNVITFGAKPDGKTDSTQSFLKAWMSACSSLTRSTINVPKGRFLL 73
           F LF+    ++S  NYNV  +GAK DG+TDS+++FL AW +AC+S   STI VP+G +L+
Sbjct: 7   FFLFLINSSLASNTNYNVQNYGAKSDGETDSSEAFLSAWSAACASTRSSTIYVPRGNYLI 66

Query: 74  TSITFRGP-CKNSNITFQLNGTLVAPLDYNALGDSRYWILFTKVNGISFIGGNIDGKGTD 133
            +  F G  C++  IT +++GTL+AP DYN +G+   WI F KVNGIS  GG  DG+G  
Sbjct: 67  RNAYFNGKTCQSKAITIRIDGTLLAPSDYNVIGNDENWIKFEKVNGISIYGGTFDGQGAA 126

Query: 134 YWACKGSG-KKCPPGARLQQCD------GQEYDCISIGDGTNNLFMSDIKCGPA--MVIG 193
            WACK S  KKCP G             G   DC+SIG GT+NL++  I CGP   + IG
Sbjct: 127 LWACKKSNNKKCPDGTTSSGVSIVNSQIGTGDDCVSIGPGTSNLWIEGIACGPGHGISIG 186

Query: 194 SLGKEVNENGVENIRLMNTVFTKSDNGVRIKTSPTSSNGFVKHVVFENITMHNVKHPILI 253
           SLG +  E GV+N+ +    F+ + NGVR+KT    SNGFV++V+F++I M NVK+PI+I
Sbjct: 187 SLGWKEQELGVQNVTIKTVTFSGTTNGVRVKTWARPSNGFVRNVLFQHIVMVNVKNPIII 246

Query: 254 DQNYCPNNQGCSVEDSGVKISDVTYKNIEGTSAT 275
           DQNYCPN++ C  + SGVKISDVTY++I GTSAT
Sbjct: 247 DQNYCPNHESCPHKGSGVKISDVTYQDIHGTSAT 280

[Close]

BLAST of Cucsa.038070 vs. TrEMBL
Match: A0A0A0M0F3_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_1G689640 PE=3 SV=1)

HSP 1 Score: 259.2 bits (661), Expect = 5.7e-66
Identity = 134/214 (62.62%), Postives = 158/214 (73.83%), Query Frame = 1


Query: 1   MANPTKSLVFVLFFHLFIQISSAGNYNVITFGAKPDGKTDSTQSFLKAWMSACSSLTRST 60
           MANPTKSLVF++FFHLFIQIS+A NYNVI+FGAKPDGKTDSTQSFLKAW SACSS TRST
Sbjct: 1   MANPTKSLVFIVFFHLFIQISTAANYNVISFGAKPDGKTDSTQSFLKAWTSACSSSTRST 60

Query: 61  INVPKGRFLLTSITFRGPCKNSNITFQLNGTLVAPLDYNALGDSRYWILFTKVNGISFIG 120
           INVPKGRFLLT ITFRGPCKN  ITF LNGTLVAPLDY ALGDS YWILF KV+G+SF+G
Sbjct: 61  INVPKGRFLLTPITFRGPCKN-KITFLLNGTLVAPLDYRALGDSGYWILFIKVDGVSFVG 120

Query: 121 GNIDGKGTDYWACKGSGKKCPPGARLQQCDGQEYDCISIG-DGTNNLFMSDIKCGPAMVI 180
           G IDGKGT YWACK S K CPPGAR            SI  +  NN+ +S +    +   
Sbjct: 121 GTIDGKGTAYWACKNSAKNCPPGAR------------SITFNWANNIILSGLTSINSQQT 180

Query: 181 GSLGKEVNENGVENIRLMNTVFTKSDNGVRIKTS 214
             +    N   V+N+++M    + + +G+ +++S
Sbjct: 181 HVVINSCNNVVVKNVKIMAPDQSPNTDGIHVQSS 201

[Close]

BLAST of Cucsa.038070 vs. TrEMBL
Match: A0A0A0M0F3_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_1G689640 PE=3 SV=1)

HSP 1 Score: 202.2 bits (513), Expect = 8.3e-49
Identity = 97/124 (78.23%), Postives = 110/124 (88.71%), Query Frame = 1


Query: 155 DCISIGDGTNNLFMSDIKCGPA--MVIGSLGKEVNENGVENIRLMNTVFTKSDNGVRIKT 214
           DCISIGDGT NLFMSDIKCGP   + IGSLGKE NENGVEN+ L+N VFTKSDNGVRIKT
Sbjct: 216 DCISIGDGTKNLFMSDIKCGPGHGVSIGSLGKEANENGVENVTLINAVFTKSDNGVRIKT 275

Query: 215 SPTSSNGFVKHVVFENITMHNVKHPILIDQNYCPNNQGCSVEDSGVKISDVTYKNIEGTS 274
            PT SNGFV+HV+F+NI M NVK+PILIDQNYCPN+Q CS++ SGVKI+DVTYK+IEGTS
Sbjct: 276 WPTPSNGFVRHVIFQNIVMINVKNPILIDQNYCPNHQSCSLQSSGVKINDVTYKSIEGTS 335

Query: 275 ATAE 277
           AT+E
Sbjct: 336 ATSE 339

HSP 2 Score: 254.6 bits (649), Expect = 1.4e-64
Identity = 134/250 (53.60%), Postives = 169/250 (67.60%), Query Frame = 1


Query: 27  NVITFGAKPDGKTDSTQSFLKAWMSACSSLTRSTINVPKGRFLLTSITFRGPCKNSNITF 86
           NV+ FGAK DGK+D T++FLKAW SAC+S + +TI VPKGR+LL +  FRGPCK   +TF
Sbjct: 9   NVVNFGAKADGKSDCTKAFLKAWESACTSQSPATIYVPKGRYLLRNTNFRGPCKRK-VTF 68

Query: 87  QLNGTLVAPLDYNALGDSRYWILFTKVNGISFIGGNIDGKGTDYWACKGSGKKCPPGARL 146
            ++GTLVAP DY ALG+S YWILF  V  I   GG +D KG  +W C+ SGK  P  ++ 
Sbjct: 69  LIDGTLVAPEDYRALGNSGYWILFNHVENIVVSGGKLDAKGAAFWNCRRSGKGLPRWSKG 128

Query: 147 QQCDGQEYDCISIGDGTNNLFMSDIKCGPAMVIGSLGKEVNENGVENIRLMNTVFTKSDN 206
                  Y C +I      LF+  I       IGSLGKE+ E GVEN+ L N +F+ SD+
Sbjct: 129 M------YMC-AIFPMPMLLFLF-INYKKLSNIGSLGKEMKEQGVENVTLTNAIFSGSDS 188

Query: 207 GVRIKTSPTSSNGFVKHVVFENITMHNVKHPILIDQNYCPNNQGCSVEDSGVKISDVTYK 266
           GVRIKT    S GFV++V F+NI M NV++PI+IDQNYCPNNQGC  + SG+K S +TY 
Sbjct: 189 GVRIKTWARPSKGFVRNVTFQNIIMDNVENPIIIDQNYCPNNQGCPGQTSGIKSSQITYS 248

Query: 267 NIEGTSATAE 277
           N+ GTSAT E
Sbjct: 249 NMNGTSATPE 249

[Close]

BLAST of Cucsa.038070 vs. TrEMBL
Match: B9SSX4_RICCO (Polygalacturonase, putative OS=Ricinus communis GN=RCOM_1267850 PE=3 SV=1)

HSP 1 Score: 248.4 bits (633), Expect = 1.0e-62
Identity = 127/278 (45.68%), Postives = 174/278 (62.59%), Query Frame = 1


Query: 1   MANPTKS-LVFVLFFHLFIQISS---AGNYNVITFGAKPDGKTDSTQSFLKAWMSACSSL 60
           MA P  + LV +    +F+  SS   A  YNV ++GAKPDG TDST++F+ AW  ACSSL
Sbjct: 1   MALPVSTRLVVLAIIFVFVTSSSFATAAIYNVSSYGAKPDGTTDSTEAFIAAWKQACSSL 60

Query: 61  TRSTINVPKGRFLLTSITFRGPCKNSNITFQLNGTLVAPLDYNALGDSRYWILFTKVNGI 120
             +TI VP GRFLL ++ FRG C+N+ I  +++G LVAP DY  +G S  W+LF  V+G+
Sbjct: 61  EPTTIYVPLGRFLLRNVAFRGKCRNNAIQIRIDGMLVAPSDYRVIGHSENWLLFENVDGV 120

Query: 121 SFIGGNIDGKGTDYWACKGSGKKCPPGARLQQCDGQEYDCISIGDGTNNLFMSDIKCGPA 180
           SF GG +D +GT  W CK + K CP GA   +               NN+ +  +    +
Sbjct: 121 SFFGGVLDAQGTGLWNCKATAKNCPSGATSLRF-----------TNANNIEIKGLSSLNS 180

Query: 181 MVIGSLGKEVNENGVENIRLMNTVFTKSDNGVRIKTSPTSSNGFVKHVVFENITMHNVKH 240
             IGSLGKE +E GV+N+ +    FT ++NGVRIK     S GF +++ F+N  M NV +
Sbjct: 181 QFIGSLGKEFHEPGVQNVTVKTVTFTGTENGVRIKAWARPSTGFARNISFQNAIMKNVHN 240

Query: 241 PILIDQNYCPNNQGCSVEDSGVKISDVTYKNIEGTSAT 275
           PI+IDQNYCP+ + C  + SG+KIS+V Y+NI GTSAT
Sbjct: 241 PIVIDQNYCPHKRNCPHQGSGIKISNVCYQNIYGTSAT 267

[Close]

BLAST of Cucsa.038070 vs. TAIR10
Match: AT2G43890.1 (AT2G43890.1 Pectin lyase-like superfamily protein)

HSP 1 Score: 174.1 bits (440), Expect = 1.2e-43
Identity = 83/148 (56.08%), Postives = 104/148 (70.27%), Query Frame = 1


Query: 1   MANPTKSLVFVLFFHLFIQISS---AGNYNVITFGAKPDGKTDSTQSFLKAWMSACSSLT 60
           M N     V ++FF  F+ + S   A NYNV++FGAKPDG+TDST++FL AW +AC S  
Sbjct: 1   MDNNKLVAVLLMFFSSFLLMKSSTAASNYNVVSFGAKPDGRTDSTKAFLGAWQAACRSAA 60

Query: 61  RSTINVPKGRFLLTSITFRGPCKNSNITFQLNGTLVAPLDYNALGDSRYWILFTKVNGIS 120
             T+ VP+G FLL  + FRGPC+ S ITFQ+ GT+VAP DY  LG+S YWILF KVN IS
Sbjct: 61  AVTVTVPRGSFLLKPVEFRGPCR-SRITFQIYGTIVAPSDYRGLGNSGYWILFVKVNRIS 120

Query: 121 FIGGNIDGKGTDYWACKGSGKKCPPGAR 146
            IGG +D +G  +WAC+ SGK CP GAR
Sbjct: 121 IIGGTLDARGASFWACRKSGKSCPVGAR 147

HSP 2 Score: 161.8 bits (408), Expect = 6.3e-40
Identity = 76/124 (61.29%), Postives = 98/124 (79.03%), Query Frame = 1


Query: 155 DCISIGDGTNNLFMSDIKCGPA--MVIGSLGKEVNENGVENIRLMNTVFTKSDNGVRIKT 214
           DCISIG GT NL+MS + CGP   + IGSLG++ NE GVENI L+N+VF+ SDNGVRIKT
Sbjct: 219 DCISIGPGTRNLYMSKLNCGPGHGISIGSLGRDANEAGVENITLINSVFSGSDNGVRIKT 278

Query: 215 SPTSSNGFVKHVVFENITMHNVKHPILIDQNYCPNNQGCSVEDSGVKISDVTYKNIEGTS 274
               S GFV++V+F+N+ M NV++PI++DQNYCP+NQGC  + SGVKIS V Y+NI+GTS
Sbjct: 279 WARQSTGFVRNVLFQNLIMKNVQNPIIVDQNYCPSNQGCPKQGSGVKISQVVYRNIQGTS 338

Query: 275 ATAE 277
            T +
Sbjct: 339 RTQQ 342

[Close]

BLAST of Cucsa.038070 vs. TAIR10
Match: AT1G05660.1 (AT1G05660.1 Pectin lyase-like superfamily protein)

HSP 1 Score: 163.7 bits (413), Expect = 1.6e-40
Identity = 75/139 (53.96%), Postives = 98/139 (70.50%), Query Frame = 1


Query: 7   SLVFVLFFHLFIQISSAGNYNVITFGAKPDGKTDSTQSFLKAWMSACSSLTRSTINVPKG 66
           SL+F L   + + IS++  +NV++FGAKPDG TDST +FLKAW  AC S + +T+ VPKG
Sbjct: 9   SLLFTLLTFIDVSISASNVFNVVSFGAKPDGVTDSTGAFLKAWQGACVSASSATVVVPKG 68

Query: 67  RFLLTSITFRGPCKNSNITFQLNGTLVAPLDYNALGDSRYWILFTKVNGISFIGGNIDGK 126
            FLL  ITF GPCK S ITFQ+ GT++AP DY   G+S +WILF KVN  S +GG  D +
Sbjct: 69  TFLLKVITFGGPCK-SKITFQVAGTVIAPEDYRTFGNSGFWILFNKVNRFSLVGGTFDAR 128

Query: 127 GTDYWACKGSGKKCPPGAR 146
              +W+C+ SG+ CPPG R
Sbjct: 129 ANGFWSCRKSGQNCPPGVR 146

HSP 2 Score: 150.2 bits (378), Expect = 1.9e-36
Identity = 72/124 (58.06%), Postives = 95/124 (76.61%), Query Frame = 1


Query: 155 DCISIGDGTNNLFMSDIKCGPA--MVIGSLGKEVNENGVENIRLMNTVFTKSDNGVRIKT 214
           DC++IG GT NL ++ + CGP   + IGSL KE+ E+GVEN+ + ++VFT S NGVRIK+
Sbjct: 218 DCVAIGPGTRNLLITKLACGPGHGVSIGSLAKELKEDGVENVTVSSSVFTGSQNGVRIKS 277

Query: 215 SPTSSNGFVKHVVFENITMHNVKHPILIDQNYCPNNQGCSVEDSGVKISDVTYKNIEGTS 274
               SNGFV+ V F+++ M NV++PI+IDQNYCP ++GC  E SGVKIS VTYKNI+GTS
Sbjct: 278 WARPSNGFVRTVFFQDLVMKNVENPIIIDQNYCPTHEGCPNEYSGVKISQVTYKNIQGTS 337

Query: 275 ATAE 277
           AT E
Sbjct: 338 ATQE 341

[Close]

BLAST of Cucsa.038070 vs. TAIR10
Match: AT3G59850.1 (AT3G59850.1 Pectin lyase-like superfamily protein)

HSP 1 Score: 163.3 bits (412), Expect = 2.2e-40
Identity = 103/276 (37.32%), Postives = 148/276 (53.62%), Query Frame = 1


Query: 7   SLVFVLFFHLFIQISSAGNYNVITFGAKPDGKTDSTQSFLKAWMSACSSLTRSTINVPKG 66
           SL+ VL F L +  SSA  YN++++GAKPDGKTDST++F   W  AC+S+   TI VPKG
Sbjct: 4   SLLIVLLFLLSVSSSSAQTYNILSYGAKPDGKTDSTKAFTVLWAKACASVKPVTILVPKG 63

Query: 67  RFLLTSITFRGP-CKNSNITFQLNGTLVAPLDYNALGDSRYWILFTKVNGISFIGGNIDG 126
           RFLL SI F G  CK  ++TF++ GTLVAP DY  +G   YWILF  ++GIS  GG +D 
Sbjct: 64  RFLLRSIIFDGSKCKRKSVTFRIQGTLVAPSDYRVIGKENYWILFQHLDGISVYGGVLDA 123

Query: 127 KGTDYWACKGSGKKCPPGARLQQCDGQEYDCISIG-DGTNNLFMSDIKCGPAMVIGSLGK 186
           +G   W+CK SGK CP GA             SIG   + N+ +S +       + S   
Sbjct: 124 QGASLWSCKKSGKNCPSGA------------TSIGFQSSRNVVISGLTS-----LNSQMF 183

Query: 187 EVNENGVENIRLMNTVFTKSDN-----GVRIKTSPTSSNGFVKHVVFEN-ITMHNVKHPI 246
            V  NG  N++L     +   N     G+ +++S T S    K    ++ +++    + +
Sbjct: 184 HVAINGCSNVKLDGVKVSADGNSPNTDGIHVQSSSTVSILNSKISTGDDCVSIGPGTNGL 243

Query: 247 LIDQNYCPNNQGCSVEDSGVKISDVTYKNIEGTSAT 275
            I+   C    G S+   G +  +V  +NI   +AT
Sbjct: 244 WIENVACGPGHGISIGSLGKESVEVGVQNITVKTAT 262

HSP 2 Score: 135.6 bits (340), Expect = 4.8e-32
Identity = 65/122 (53.28%), Postives = 89/122 (72.95%), Query Frame = 1


Query: 155 DCISIGDGTNNLFMSDIKCGPA--MVIGSLGKEVNENGVENIRLMNTVFTKSDNGVRIKT 214
           DC+SIG GTN L++ ++ CGP   + IGSLGKE  E GV+NI +    FT ++NGVRIK+
Sbjct: 215 DCVSIGPGTNGLWIENVACGPGHGISIGSLGKESVEVGVQNITVKTATFTGTENGVRIKS 274

Query: 215 SPTSSNGFVKHVVFENITMHNVKHPILIDQNYCPNNQGCSVEDSGVKISDVTYKNIEGTS 274
               SNGF K++ F++  M+NV++PI+IDQNYCP N+ C  + SG+KISDV + +I GTS
Sbjct: 275 WARPSNGFAKNIRFQHCVMNNVQNPIVIDQNYCPGNENCPNQVSGIKISDVMFFDIHGTS 334

[Close]

BLAST of Cucsa.038070 vs. TAIR10
Match: AT1G05650.1 (AT1G05650.1 Pectin lyase-like superfamily protein)

HSP 1 Score: 161.8 bits (408), Expect = 6.3e-40
Identity = 101/275 (36.73%), Postives = 147/275 (53.45%), Query Frame = 1


Query: 5   TKSLV-FVLFFHL--FIQISSAGN--YNVITFGAKPDGKTDSTQSFLKAWMSACSSLTRS 64
           TKS + F L F L  FI +SS+ +  +NV++FGAKPDG TDST +FLKAW  AC S   +
Sbjct: 2   TKSAITFPLIFTLLTFIDVSSSASIVFNVVSFGAKPDGVTDSTAAFLKAWQGACGSAASA 61

Query: 65  TINVPKGRFLLTSITFRGPCKNSNITFQLNGTLVAPLDYNALGDSRYWILFTKVNGISFI 124
           T+ VP G FLL  ITF GPCK S ITFQ+ GT+VAP DY   G+S  WILF KVN  S +
Sbjct: 62  TVVVPTGTFLLKVITFGGPCK-SKITFQVTGTVVAPEDYRTFGNSGSWILFNKVNRFSLV 121

Query: 125 GGNIDGKGTDYWACKGSGKKCPPGARLQQCDGQEYDCISIGDGTNNLFMSDIKCGPAMVI 184
           GG  D +G+ +W+C+ SG+ CPPG R           IS  +   ++ +S +K   + V 
Sbjct: 122 GGTFDARGSGFWSCRKSGQNCPPGVR----------SISF-NSAKDVIISGVKSMNSQVS 181

Query: 185 GSLGKEVNENGVENIRLMNTVFTKSDNGVRIKTSP-TSSNGFVKHVVFENITMHNVKHPI 244
                      V NIRL+    + + +G  ++ S   +  G       + + +       
Sbjct: 182 HMTLNGCTNVAVRNIRLVAPGDSPNTDGFTVQFSTGVTLTGSTVQTGDDCVAIGQGTRNF 241

Query: 245 LIDQNYCPNNQGCSVEDSGVKISDVTYKNIEGTSA 274
           LI +  C    G S+     ++++   +N+  +S+
Sbjct: 242 LISKLACGPGHGVSIGSLAKQLNEDGVENVTVSSS 264

HSP 2 Score: 157.5 bits (397), Expect = 1.2e-38
Identity = 80/155 (51.61%), Postives = 107/155 (69.03%), Query Frame = 1


Query: 124 DGKGTDYWACKGSGKKCPPGARLQQCDGQEYDCISIGDGTNNLFMSDIKCGPA--MVIGS 183
           D   TD +  + S      G+ +Q  D    DC++IG GT N  +S + CGP   + IGS
Sbjct: 191 DSPNTDGFTVQFSTGVTLTGSTVQTGD----DCVAIGQGTRNFLISKLACGPGHGVSIGS 250

Query: 184 LGKEVNENGVENIRLMNTVFTKSDNGVRIKTSPTSSNGFVKHVVFENITMHNVKHPILID 243
           L K++NE+GVEN+ + ++VFT S NGVRIK+    S GFV++V F+N+ M NV++PI+ID
Sbjct: 251 LAKQLNEDGVENVTVSSSVFTGSQNGVRIKSWARPSTGFVRNVFFQNLIMRNVQNPIIID 310

Query: 244 QNYCPNNQGCSVEDSGVKISDVTYKNIEGTSATAE 277
           QNYCP+NQGC  E SGVKI+ VTYKNI+GTSAT E
Sbjct: 311 QNYCPSNQGCPTEHSGVKITQVTYKNIQGTSATQE 341

[Close]

BLAST of Cucsa.038070 vs. TAIR10
Match: AT2G43870.1 (AT2G43870.1 Pectin lyase-like superfamily protein)

HSP 1 Score: 156.8 bits (395), Expect = 2.0e-38
Identity = 73/137 (53.28%), Postives = 95/137 (69.34%), Query Frame = 1


Query: 9   VFVLFFHLFIQISSAGNYNVITFGAKPDGKTDSTQSFLKAWMSACSSLTRSTINVPKGRF 68
           + VLF   FI   SA +YNV++FGAKPDGKTD+T++F+  W +AC+S    TI VPKGRF
Sbjct: 4   LLVLFVFFFISSCSAQSYNVLSFGAKPDGKTDATKAFMAVWQTACASSRPVTIVVPKGRF 63

Query: 69  LLTSITFRG-PCKNSNITFQLNGTLVAPLDYNALGDSRYWILFTKVNGISFIGGNIDGKG 128
           LL S+TF G  CK   +TF+++GTLVAP DY  +G+  YWI F  ++GI+  GG +D +G
Sbjct: 64  LLRSVTFDGSKCKPKPVTFRIDGTLVAPADYRVIGNEDYWIFFQHLDGITVYGGVLDARG 123

Query: 129 TDYWACKGSGKKCPPGA 145
              W CK SGK CP GA
Sbjct: 124 ASLWDCKKSGKNCPSGA 140

HSP 2 Score: 129.0 bits (323), Expect = 4.5e-30
Identity = 62/122 (50.82%), Postives = 90/122 (73.77%), Query Frame = 1


Query: 155 DCISIGDGTNNLFMSDIKCGPA--MVIGSLGKEVNENGVENIRLMNTVFTKSDNGVRIKT 214
           DC+SIG GTN L++ ++ CGP   + IGSLGK+  E+GV+N+ +    FT +DNGVRIK+
Sbjct: 213 DCVSIGPGTNGLWIENVACGPGHGISIGSLGKDSVESGVQNVTVKTVTFTGTDNGVRIKS 272

Query: 215 SPTSSNGFVKHVVFENITMHNVKHPILIDQNYCPNNQGCSVEDSGVKISDVTYKNIEGTS 274
               S+GF K++ F++  M+NV++PI+IDQNYCP++  C  + SG+KISDV + +I GTS
Sbjct: 273 WARPSSGFAKNIRFQHCVMNNVENPIIIDQNYCPDHD-CPRQVSGIKISDVLFVDIHGTS 332

[Close]

BLAST of Cucsa.038070 vs. NCBI nr
Match: gi|700198669|gb|KGN53827.1| (hypothetical protein Csa_4G153300 [Cucumis sativus])

HSP 1 Score: 305.4 bits (781), Expect = 9.9e-80
Identity = 147/154 (95.45%), Postives = 149/154 (96.75%), Query Frame = 1


Query: 1   MANPTKSLVFVLFFHLFIQISSAGNYNVITFGAKPDGKTDSTQSFLKAWMSACSSLTRST 60
           MANPTKSLVFVLFFHLFIQISSAGNYNVITFGAKPDGKTDSTQSFLKAWMSACSSLTRST
Sbjct: 1   MANPTKSLVFVLFFHLFIQISSAGNYNVITFGAKPDGKTDSTQSFLKAWMSACSSLTRST 60

Query: 61  INVPKGRFLLTSITFRGPCKNSNITFQLNGTLVAPLDYNALGDSRYWILFTKVNGISFIG 120
           INVPKGRFLLTSITFRGPCKNSNITFQLNGTLVAPLDYNALGDSRYWILFTKVNGISFIG
Sbjct: 61  INVPKGRFLLTSITFRGPCKNSNITFQLNGTLVAPLDYNALGDSRYWILFTKVNGISFIG 120

Query: 121 GNIDGKGTDYWACKGSGKKCPPGARLQQCDGQEY 155
           GNIDGKGTDYWACKGSGKKCPPGAR  +   Q+Y
Sbjct: 121 GNIDGKGTDYWACKGSGKKCPPGARKAEKKHQKY 154

[Close]

BLAST of Cucsa.038070 vs. NCBI nr
Match: gi|659121741|ref|XP_008460791.1| (PREDICTED: polygalacturonase-like [Cucumis melo])

HSP 1 Score: 279.6 bits (714), Expect = 5.8e-72
Identity = 149/270 (55.19%), Postives = 188/270 (69.63%), Query Frame = 1


Query: 1   MANPTKSLVFVLFFHLFIQISSAGNYNVITFGAKPDGKTDSTQSFLKAWMSACSSLTRST 60
           MAN  KSLVF++FFHLFIQISSAG+YNVITFGAKPDGKTDSTQSFLKAW SACSSLTRST
Sbjct: 1   MANSRKSLVFIVFFHLFIQISSAGSYNVITFGAKPDGKTDSTQSFLKAWKSACSSLTRST 60

Query: 61  INVPKGRFLLTSITFRGPCKNSNITFQLNGTLVAPLDYNALGDSRYWILFTKVNGISFIG 120
           INVPKGRFLLT+ITFRGPCKN+NI F LNGTLVAPLDYNALGDS YWILFTKVNG+SFIG
Sbjct: 61  INVPKGRFLLTAITFRGPCKNNNIIFLLNGTLVAPLDYNALGDSGYWILFTKVNGVSFIG 120

Query: 121 GNIDGKGTDYWACKGSGKKCPPGARLQQCDGQEYDCISIG-DGTNNLFMSDIKCGPAMVI 180
           GNIDGKGT YWACKGSGKKCPPGAR            SI  +  +N+ +S +    +   
Sbjct: 121 GNIDGKGTAYWACKGSGKKCPPGAR------------SITFNWASNIIISGLTSINSRQT 180

Query: 181 GSLGKEVNENGVENIRLMNTVFTKSDNGVRIKTSP-TSSNGFVKHVVFENITMHNVKHPI 240
             +    N   V+N+++M    + + +G+ +++S   + +G       + I++ +    +
Sbjct: 181 HIVINSCNNVVVKNVKIMAPDQSPNTDGIHVQSSTGVTISGTTIKTGDDCISIGDGTKNL 240

Query: 241 LIDQNYCPNNQGCSVEDSGVKISDVTYKNI 269
            +    C    G S+   G ++++   +N+
Sbjct: 241 FMSDIKCGPGHGVSIGSLGKEVNENGVENV 258

[Close]

BLAST of Cucsa.038070 vs. NCBI nr
Match: gi|659121741|ref|XP_008460791.1| (PREDICTED: polygalacturonase-like [Cucumis melo])

HSP 1 Score: 213.4 bits (542), Expect = 5.1e-52
Identity = 105/124 (84.68%), Postives = 112/124 (90.32%), Query Frame = 1


Query: 155 DCISIGDGTNNLFMSDIKCGPA--MVIGSLGKEVNENGVENIRLMNTVFTKSDNGVRIKT 214
           DCISIGDGT NLFMSDIKCGP   + IGSLGKEVNENGVEN+ L+N VFTKSDNGVRIKT
Sbjct: 217 DCISIGDGTKNLFMSDIKCGPGHGVSIGSLGKEVNENGVENVTLVNAVFTKSDNGVRIKT 276

Query: 215 SPTSSNGFVKHVVFENITMHNVKHPILIDQNYCPNNQGCSVEDSGVKISDVTYKNIEGTS 274
            PT SNGFVKHVVFENI M++VKHPILIDQNYCPNNQ CSV+ SGVKISDVTYKNIEGTS
Sbjct: 277 WPTPSNGFVKHVVFENIIMNDVKHPILIDQNYCPNNQRCSVQGSGVKISDVTYKNIEGTS 336

Query: 275 ATAE 277
           AT+E
Sbjct: 337 ATSE 340

HSP 2 Score: 259.2 bits (661), Expect = 8.2e-66
Identity = 134/214 (62.62%), Postives = 158/214 (73.83%), Query Frame = 1


Query: 1   MANPTKSLVFVLFFHLFIQISSAGNYNVITFGAKPDGKTDSTQSFLKAWMSACSSLTRST 60
           MANPTKSLVF++FFHLFIQIS+A NYNVI+FGAKPDGKTDSTQSFLKAW SACSS TRST
Sbjct: 1   MANPTKSLVFIVFFHLFIQISTAANYNVISFGAKPDGKTDSTQSFLKAWTSACSSSTRST 60

Query: 61  INVPKGRFLLTSITFRGPCKNSNITFQLNGTLVAPLDYNALGDSRYWILFTKVNGISFIG 120
           INVPKGRFLLT ITFRGPCKN  ITF LNGTLVAPLDY ALGDS YWILF KV+G+SF+G
Sbjct: 61  INVPKGRFLLTPITFRGPCKN-KITFLLNGTLVAPLDYRALGDSGYWILFIKVDGVSFVG 120

Query: 121 GNIDGKGTDYWACKGSGKKCPPGARLQQCDGQEYDCISIG-DGTNNLFMSDIKCGPAMVI 180
           G IDGKGT YWACK S K CPPGAR            SI  +  NN+ +S +    +   
Sbjct: 121 GTIDGKGTAYWACKNSAKNCPPGAR------------SITFNWANNIILSGLTSINSQQT 180

Query: 181 GSLGKEVNENGVENIRLMNTVFTKSDNGVRIKTS 214
             +    N   V+N+++M    + + +G+ +++S
Sbjct: 181 HVVINSCNNVVVKNVKIMAPDQSPNTDGIHVQSS 201

[Close]

BLAST of Cucsa.038070 vs. NCBI nr
Match: gi|778665426|ref|XP_004142576.2| (PREDICTED: polygalacturonase-like [Cucumis sativus])

HSP 1 Score: 202.2 bits (513), Expect = 1.2e-48
Identity = 97/124 (78.23%), Postives = 110/124 (88.71%), Query Frame = 1


Query: 155 DCISIGDGTNNLFMSDIKCGPA--MVIGSLGKEVNENGVENIRLMNTVFTKSDNGVRIKT 214
           DCISIGDGT NLFMSDIKCGP   + IGSLGKE NENGVEN+ L+N VFTKSDNGVRIKT
Sbjct: 216 DCISIGDGTKNLFMSDIKCGPGHGVSIGSLGKEANENGVENVTLINAVFTKSDNGVRIKT 275

Query: 215 SPTSSNGFVKHVVFENITMHNVKHPILIDQNYCPNNQGCSVEDSGVKISDVTYKNIEGTS 274
            PT SNGFV+HV+F+NI M NVK+PILIDQNYCPN+Q CS++ SGVKI+DVTYK+IEGTS
Sbjct: 276 WPTPSNGFVRHVIFQNIVMINVKNPILIDQNYCPNHQSCSLQSSGVKINDVTYKSIEGTS 335

Query: 275 ATAE 277
           AT+E
Sbjct: 336 ATSE 339

HSP 2 Score: 254.6 bits (649), Expect = 2.0e-64
Identity = 134/250 (53.60%), Postives = 169/250 (67.60%), Query Frame = 1


Query: 27  NVITFGAKPDGKTDSTQSFLKAWMSACSSLTRSTINVPKGRFLLTSITFRGPCKNSNITF 86
           NV+ FGAK DGK+D T++FLKAW SAC+S + +TI VPKGR+LL +  FRGPCK   +TF
Sbjct: 9   NVVNFGAKADGKSDCTKAFLKAWESACTSQSPATIYVPKGRYLLRNTNFRGPCKRK-VTF 68

Query: 87  QLNGTLVAPLDYNALGDSRYWILFTKVNGISFIGGNIDGKGTDYWACKGSGKKCPPGARL 146
            ++GTLVAP DY ALG+S YWILF  V  I   GG +D KG  +W C+ SGK  P  ++ 
Sbjct: 69  LIDGTLVAPEDYRALGNSGYWILFNHVENIVVSGGKLDAKGAAFWNCRRSGKGLPRWSKG 128

Query: 147 QQCDGQEYDCISIGDGTNNLFMSDIKCGPAMVIGSLGKEVNENGVENIRLMNTVFTKSDN 206
                  Y C +I      LF+  I       IGSLGKE+ E GVEN+ L N +F+ SD+
Sbjct: 129 M------YMC-AIFPMPMLLFLF-INYKKLSNIGSLGKEMKEQGVENVTLTNAIFSGSDS 188

Query: 207 GVRIKTSPTSSNGFVKHVVFENITMHNVKHPILIDQNYCPNNQGCSVEDSGVKISDVTYK 266
           GVRIKT    S GFV++V F+NI M NV++PI+IDQNYCPNNQGC  + SG+K S +TY 
Sbjct: 189 GVRIKTWARPSKGFVRNVTFQNIIMDNVENPIIIDQNYCPNNQGCPGQTSGIKSSQITYS 248

Query: 267 NIEGTSATAE 277
           N+ GTSAT E
Sbjct: 249 NMNGTSATPE 249

[Close]

BLAST of Cucsa.038070 vs. NCBI nr
Match: gi|727604446|ref|XP_010475274.1| (PREDICTED: polygalacturonase-like isoform X2 [Camelina sativa])

HSP 1 Score: 251.1 bits (640), Expect = 2.2e-63
Identity = 141/304 (46.38%), Postives = 187/304 (61.51%), Query Frame = 1


Query: 5   TKSLVFVLFFHLFIQISSAGN-YNVITFGAKPDGKTDSTQSFLKAWMSACSSLTRSTINV 64
           T  L+F L   L    SSA N +NV++FGAKPDG TDST +FLKAW  AC S   +T+ V
Sbjct: 7   TFPLLFTLVTTLINVSSSASNVFNVVSFGAKPDGVTDSTAAFLKAWQGACGSAASATVVV 66

Query: 65  PKGRFLLTSITFRGPCKNSNITFQLNGTLVAPLDYNALGDSRYWILFTK----------- 124
           PKG FLL +ITF GPCK S ITFQ++GT+VAP DY A G+S YWILF K           
Sbjct: 67  PKGTFLLKAITFGGPCK-SKITFQVSGTVVAPADYRAFGNSGYWILFNKSISFNSAKDVI 126

Query: 125 VNGISFIGGNIDG---KGTDYWACKGSGKKCP------PGARLQQCDGQEY--------- 184
           ++G+  +   +      G      +      P       G  +Q   G  +         
Sbjct: 127 ISGVKSMNSQVTHMTLNGCTNVVVRNVKLVAPGNSPNTDGFHVQFSTGVTFTGSTVQTGD 186

Query: 185 DCISIGDGTNNLFMSDIKCGP--AMVIGSLGKEVNENGVENIRLMNTVFTKSDNGVRIKT 244
           DC++IG GT N  ++ + CGP   + IGSL K++ E+GVEN+ L ++VFT + NGVRIK+
Sbjct: 187 DCVAIGPGTRNFLITKLACGPGHGVSIGSLAKDLKEDGVENVTLSSSVFTGTQNGVRIKS 246

Query: 245 SPTSSNGFVKHVVFENITMHNVKHPILIDQNYCPNNQGCSVEDSGVKISDVTYKNIEGTS 277
               S G+V++V F+N+ M NV++PI+IDQNYCP++QGC  E SGVKIS VTYKNI+GTS
Sbjct: 247 WARPSTGYVRNVFFQNLIMKNVQNPIIIDQNYCPSHQGCPTEHSGVKISGVTYKNIQGTS 306

The following BLAST results are available for this feature:

BLAST of Cucsa.038070 vs. Swiss-Prot


Match Name	E-value	Identity	Description
PGLR_PRUPE	2.8e-34	51.80	Polygalacturonase OS=Prunus persica PE=2 SV=1	[more]
PGLR_GOSBA	8.0e-29	53.23	Polygalacturonase OS=Gossypium barbadense GN=G9 PE=2 SV=1	[more]
PGLR_GOSHI	8.0e-29	53.23	Polygalacturonase OS=Gossypium hirsutum GN=G9 PE=2 SV=1	[more]
PGLR6_ARATH	7.5e-27	33.18	Probable polygalacturonase At2g43860 OS=Arabidopsis thaliana GN=At2g43860 PE=2 S...	[more]
PGLR2_ARATH	1.3e-26	47.76	Exopolygalacturonase clone GBGA483 OS=Arabidopsis thaliana GN=At3g07850 PE=2 SV=...	[more]

BLAST of Cucsa.038070 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KZM5_CUCSA	6.9e-80	95.45	Uncharacterized protein OS=Cucumis sativus GN=Csa_4G153300 PE=3 SV=1	[more]
M1A9G5_SOLTU	3.6e-68	50.00	Uncharacterized protein OS=Solanum tuberosum GN=PGSC0003DMG403006909 PE=3 SV=1	[more]
A0A0A0M0F3_CUCSA	5.7e-66	62.62	Uncharacterized protein OS=Cucumis sativus GN=Csa_1G689640 PE=3 SV=1	[more]
A0A0A0M0F3_CUCSA	8.3e-49	78.23	Uncharacterized protein OS=Cucumis sativus GN=Csa_1G689640 PE=3 SV=1	[more]
B9SSX4_RICCO	1.0e-62	45.68	Polygalacturonase, putative OS=Ricinus communis GN=RCOM_1267850 PE=3 SV=1	[more]

BLAST of Cucsa.038070 vs. TAIR10


Match Name	E-value	Identity	Description
AT2G43890.1	1.2e-43	56.08	Pectin lyase-like superfamily protein	[more]
AT1G05660.1	1.6e-40	53.96	Pectin lyase-like superfamily protein	[more]
AT3G59850.1	2.2e-40	37.32	Pectin lyase-like superfamily protein	[more]
AT1G05650.1	6.3e-40	36.73	Pectin lyase-like superfamily protein	[more]
AT2G43870.1	2.0e-38	53.28	Pectin lyase-like superfamily protein	[more]

BLAST of Cucsa.038070 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|700198669\|gb\|KGN53827.1\|	9.9e-80	95.45	hypothetical protein Csa_4G153300 [Cucumis sativus]	[more]
gi\|659121741\|ref\|XP_008460791.1\|	5.8e-72	55.19	PREDICTED: polygalacturonase-like [Cucumis melo]	[more]
gi\|659121741\|ref\|XP_008460791.1\|	5.1e-52	84.68	PREDICTED: polygalacturonase-like [Cucumis melo]	[more]
gi\|778665426\|ref\|XP_004142576.2\|	1.2e-48	78.23	PREDICTED: polygalacturonase-like [Cucumis sativus]	[more]
gi\|727604446\|ref\|XP_010475274.1\|	2.2e-63	46.38	PREDICTED: polygalacturonase-like isoform X2 [Camelina sativa]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR000743	Glyco_hydro_28
IPR011050	Pectin_lyase_fold/virulence
IPR012334	Pectin_lyas_fold

Vocabulary: **Molecular Function**
Term	Definition
GO:0004650	polygalacturonase activity

Vocabulary: **Biological Process**
Term	Definition
GO:0005975	carbohydrate metabolic process

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0071555	cell wall organization
biological_process	GO:0005982	starch metabolic process
biological_process	GO:0005985	sucrose metabolic process
biological_process	GO:0005975	carbohydrate metabolic process
cellular_component	GO:0005576	extracellular region
molecular_function	GO:0004650	polygalacturonase activity

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cucsa.038070.1	Cucsa.038070.1	mRNA

InterPro

Analysis Name: InterPro Annotations of cucumber (Gy14)
Date Performed: 2017-01-17


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000743	Glycoside hydrolase, family 28	PFAM	PF00295	Glyco_hydro_28	coord: 155..276 score: 2.0E-32coord: 53..140 score: 2.5
IPR011050	Pectin lyase fold/virulence factor	unknown	SSF51126	Pectin lyase-like	coord: 11..274 score: 5.06
IPR012334	Pectin lyase fold	GENE3D	G3DSA:2.160.20.10		coord: 154..276 score: 1.2E-37coord: 12..151 score: 1.6
None	No IPR available	PANTHER	PTHR31375	FAMILY NOT NAMED	coord: 1..276 score: 2.6E
None	No IPR available	PANTHER	PTHR31375:SF31	F3F20.10 PROTEIN-RELATED	coord: 1..276 score: 2.6E

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Cucsa.038070	Csa4G153300	Cucumber (Chinese Long) v2	cgycuB011
Cucsa.038070	CSPI04G10420	Wild cucumber (PI 183967)	cgycpiB011
Cucsa.038070	MELO3C023170.2	Melon (DHL92) v3.6.1	cgymedB013
Cucsa.038070	CsaV3_4G010570	Cucumber (Chinese Long) v3	cgycucB014

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Cucsa.038070	Watermelon (97103) v2	cgywmbB013
Cucsa.038070	Wax gourd	cgywgoB013
Cucsa.038070	Watermelon (Charleston Gray)	cgywcgB013
Cucsa.038070	Melon (DHL92) v3.5.1	cgymeB011
Cucsa.038070	Bottle gourd (USVL1VR-Ls)	cgylsiB012