Csa4G153300 (gene) Cucumber (Chinese Long) v2

Overview
NameCsa4G153300
Typegene
OrganismCucumis. sativus (Cucumber (Chinese Long) v2)
DescriptionPolygalacturonase, putative; contains IPR000743 (Glycoside hydrolase, family 28), IPR011050 (Pectin lyase fold/virulence factor)
LocationChr4 : 8291636 .. 8292183 (-)
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCCAACCCAACAAAAAGTTTAGTCTTCGTTCTCTTCTTCCATTTGTTCATTCAAATTTCAAGTGCAGGCAACTACAATGTGATAACATTCGGTGCAAAACCGGATGGAAAAACTGACTCAACCCAATCATTTCTGAAAGCGTGGATGTCTGCATGTAGCTCCTTGACACGATCCACCATCAACGTCCCTAAGGGAAGGTTCTTGCTTACATCCATCACATTTAGAGGTCCATGTAAGAACAGCAACATTACATTTCAATTGAATGGAACACTCGTGGCACCTCTTGACTATAATGCTCTTGGAGATTCTAGGTATTGGATTTTGTTCACCAAAGTTAATGGAATTTCTTTCATCGGTGGCAATATTGATGGAAAAGGCACCGACTATTGGGCTTGCAAGGGCTCTGGAAAAAAATGTCCCCCGGGAGCAAGGGTATGCGTGTTAATTACTCAATACTTTATTGAATTCCAAAACTGCACATCGACTATCTTCATTAGAAAGCAGAAAAAAAACACCAAAAATATTACTTTTTACCCAAAAATTGA

mRNA sequence

ATGGCCAACCCAACAAAAAGTTTAGTCTTCGTTCTCTTCTTCCATTTGTTCATTCAAATTTCAAGTGCAGGCAACTACAATGTGATAACATTCGGTGCAAAACCGGATGGAAAAACTGACTCAACCCAATCATTTCTGAAAGCGTGGATGTCTGCATGTAGCTCCTTGACACGATCCACCATCAACGTCCCTAAGGGAAGGTTCTTGCTTACATCCATCACATTTAGAGGTCCATGTAAGAACAGCAACATTACATTTCAATTGAATGGAACACTCGTGGCACCTCTTGACTATAATGCTCTTGGAGATTCTAGGTATTGGATTTTGTTCACCAAAGTTAATGGAATTTCTTTCATCGGTGGCAATATTGATGGAAAAGGCACCGACTATTGGGCTTGCAAGGGCTCTGGAAAAAAATGTCCCCCGGGAGCAAGGAAAGCAGAAAAAAAACACCAAAAATATTACTTTTTACCCAAAAATTGA

Coding sequence (CDS)

ATGGCCAACCCAACAAAAAGTTTAGTCTTCGTTCTCTTCTTCCATTTGTTCATTCAAATTTCAAGTGCAGGCAACTACAATGTGATAACATTCGGTGCAAAACCGGATGGAAAAACTGACTCAACCCAATCATTTCTGAAAGCGTGGATGTCTGCATGTAGCTCCTTGACACGATCCACCATCAACGTCCCTAAGGGAAGGTTCTTGCTTACATCCATCACATTTAGAGGTCCATGTAAGAACAGCAACATTACATTTCAATTGAATGGAACACTCGTGGCACCTCTTGACTATAATGCTCTTGGAGATTCTAGGTATTGGATTTTGTTCACCAAAGTTAATGGAATTTCTTTCATCGGTGGCAATATTGATGGAAAAGGCACCGACTATTGGGCTTGCAAGGGCTCTGGAAAAAAATGTCCCCCGGGAGCAAGGAAAGCAGAAAAAAAACACCAAAAATATTACTTTTTACCCAAAAATTGA

Protein sequence

MANPTKSLVFVLFFHLFIQISSAGNYNVITFGAKPDGKTDSTQSFLKAWMSACSSLTRSTINVPKGRFLLTSITFRGPCKNSNITFQLNGTLVAPLDYNALGDSRYWILFTKVNGISFIGGNIDGKGTDYWACKGSGKKCPPGARKAEKKHQKYYFLPKN*
Homology
[Close]
BLAST of Csa4G153300 vs. Swiss-Prot
Match: PGLR_PRUPE (Polygalacturonase OS=Prunus persica PE=2 SV=1)

HSP 1 Score: 147.1 bits (370), Expect = 1.6e-34
Identity = 72/139 (51.80%), Postives = 90/139 (64.75%), Query Frame = 1 

Query: 7   SLVFVLFFHLFIQISSAGNYNVITFGAKPDGKTDSTQSFLKAWMSACSSLTRSTINVPKG 66
           SL+FV  F +   I+S   YNV + GAK DGKTDST++FL AW  AC+S+    I VP G
Sbjct: 11  SLIFV--FMINSAIASPLTYNVASLGAKADGKTDSTKAFLSAWAKACASMNPGVIYVPAG 70

Query: 67  RFLLTSITFRGPCKNSNITFQLNGTLVAPLDYNALGDSRYWILFTKVNGISFIGGNIDGK 126
            F L  + F GPCKN+ ITF++ GTLVAP DY  +G++  WI F  VNG++  GG +DG+
Sbjct: 71  TFFLRDVVFSGPCKNNAITFRIAGTLVAPSDYRVIGNAANWIFFHHVNGVTISGGILDGQ 130

Query: 127 GTDYWACKG-SGKKCPPGA 145
           GT  WACK   G+ CP GA
Sbjct: 131 GTALWACKACHGESCPSGA 147

[Close]
BLAST of Csa4G153300 vs. Swiss-Prot
Match: PGLR6_ARATH (Probable polygalacturonase At2g43860 OS=Arabidopsis thaliana GN=At2g43860 PE=2 SV=1)

HSP 1 Score: 120.9 bits (302), Expect = 1.2e-26
Identity = 56/121 (46.28%), Postives = 79/121 (65.29%), Query Frame = 1 

Query: 27  NVITFGAKPDGKTDSTQSFLKAWMSACSSLTRSTINVPKGRFLLTSITFRG-PCKNSNIT 86
           NV+++GAKPDG  DST++FL AW  AC+S   +TI VPKGRFL+ ++ F G  CK + I+
Sbjct: 34  NVLSYGAKPDGSKDSTKAFLAAWDVACASANPTTIIVPKGRFLVGNLVFHGNECKQAPIS 93

Query: 87  FQLNGTLVAPLDYNALGDSRYWILFTKVNGISFIGGNIDGKGTDYWACKGS-GKKCPPGA 146
            ++ G++VAP D+  +  S++WI F  V  +S  GG +D +GT  W CK + G  CP GA
Sbjct: 94  IRIAGSIVAPEDFRIIASSKHWIWFEDVTDVSIYGGILDAQGTSLWKCKNNGGHNCPTGA 153

[Close]
BLAST of Csa4G153300 vs. Swiss-Prot
Match: PGLR3_ARATH (Probable polygalacturonase At3g15720 OS=Arabidopsis thaliana GN=At3g15720 PE=1 SV=1)

HSP 1 Score: 97.8 bits (242), Expect = 1.1e-19
Identity = 56/134 (41.79%), Postives = 75/134 (55.97%), Query Frame = 1 

Query: 6   KSLVFVLFFHLFIQISSAGN-YNVITFGAKPDGKTDSTQSFLKAWMSACSSLTRSTINVP 65
           K   F+ F   F+QI ++ N  +V  FGA  DG TD +Q+FLKAW + CS        VP
Sbjct: 3   KKTWFLNFSLFFLQIFTSSNALDVTQFGAVGDGVTDDSQAFLKAWEAVCSGTGDGQFVVP 62

Query: 66  KGR-FLLTSITFRGPCKNSNITFQLNGTLVAPLDYNALGDSRYWILFTKVNGISFIG-GN 125
            G  F+L  + F+G CK++ +  Q+ G LVAP   N  GD   WILFT + G+   G G 
Sbjct: 63  AGMTFMLQPLKFQGSCKSTPVFVQMLGKLVAPSKGNWKGDKDQWILFTDIEGLVIEGDGE 122

Query: 126 IDGKGTDYWACKGS 137
           I+G+G+ +W  KGS
Sbjct: 123 INGQGSSWWEHKGS 136

[Close]
BLAST of Csa4G153300 vs. Swiss-Prot
Match: ADPG1_ARATH (Polygalacturonase ADPG1 OS=Arabidopsis thaliana GN=ADPG1 PE=2 SV=1)

HSP 1 Score: 93.2 bits (230), Expect = 2.8e-18
Identity = 53/118 (44.92%), Postives = 68/118 (57.63%), Query Frame = 1 

Query: 23  AGNYNVITFGAKPDGKTDSTQSFLKAWMSACSSLTRSTINVPKGR-FLLTSITFRGPCKN 82
           A   +V  FGAK DGKTD TQ+F KAW  ACS+   +T  VPKG+ +LL S  FRGPCK+
Sbjct: 65  ASTVSVSNFGAKGDGKTDDTQAFKKAWKKACSTNGVTTFLVPKGKTYLLKSTRFRGPCKS 124

Query: 83  SNITFQLNGTLVAPLDYNALGDSRYWILFTKVNGISFIGGN---IDGKGTDYW--ACK 135
               FQ+ GTL A    +   D  +W++   VN +S  GG+   I+G G  +W  +CK
Sbjct: 125 LR-NFQILGTLSASTKRSDYKDKNHWLILEDVNNLSIDGGSTGIINGNGKTWWQNSCK 181

[Close]
BLAST of Csa4G153300 vs. Swiss-Prot
Match: PGLR1_MAIZE (Exopolygalacturonase OS=Zea mays GN=PG1 PE=1 SV=1)

HSP 1 Score: 91.7 bits (226), Expect = 8.1e-18
Identity = 45/117 (38.46%), Postives = 67/117 (57.26%), Query Frame = 1 

Query: 24  GNYNVITFGAKPDGKTDSTQSFLKAWMSACSSLTRSTINVPKGRFLLTSITFRGPCKNSN 83
           G++++   GA  +GKTDST++  +AW SAC    + TI +PKG FL+  + F GPCK  +
Sbjct: 39  GSFDITKLGASGNGKTDSTKAVQEAWASACGGTGKQTILIPKGDFLVGQLNFTGPCK-GD 98

Query: 84  ITFQLNGTLVAPLDYNALGDSRYWILFTKVNGISFIG-GNIDGKGTDYWACKGSGKK 140
           +T Q++G L+A  D +   D   WI   +V+ +   G GN+DG+G   W+     KK
Sbjct: 99  VTIQVDGNLLATTDLSQYKDHGNWIEILRVDNLVITGKGNLDGQGPAVWSKNSCTKK 154

[Close]
BLAST of Csa4G153300 vs. TrEMBL
Match: A0A0A0KZM5_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_4G153300 PE=3 SV=1)

HSP 1 Score: 335.5 bits (859), Expect = 3.6e-89
Identity = 160/160 (100.00%), Postives = 160/160 (100.00%), Query Frame = 1 

Query: 1   MANPTKSLVFVLFFHLFIQISSAGNYNVITFGAKPDGKTDSTQSFLKAWMSACSSLTRST 60
           MANPTKSLVFVLFFHLFIQISSAGNYNVITFGAKPDGKTDSTQSFLKAWMSACSSLTRST
Sbjct: 1   MANPTKSLVFVLFFHLFIQISSAGNYNVITFGAKPDGKTDSTQSFLKAWMSACSSLTRST 60

Query: 61  INVPKGRFLLTSITFRGPCKNSNITFQLNGTLVAPLDYNALGDSRYWILFTKVNGISFIG 120
           INVPKGRFLLTSITFRGPCKNSNITFQLNGTLVAPLDYNALGDSRYWILFTKVNGISFIG
Sbjct: 61  INVPKGRFLLTSITFRGPCKNSNITFQLNGTLVAPLDYNALGDSRYWILFTKVNGISFIG 120

Query: 121 GNIDGKGTDYWACKGSGKKCPPGARKAEKKHQKYYFLPKN 161
           GNIDGKGTDYWACKGSGKKCPPGARKAEKKHQKYYFLPKN
Sbjct: 121 GNIDGKGTDYWACKGSGKKCPPGARKAEKKHQKYYFLPKN 160

[Close]
BLAST of Csa4G153300 vs. TrEMBL
Match: A0A0A0M0F3_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_1G689640 PE=3 SV=1)

HSP 1 Score: 256.9 bits (655), Expect = 1.6e-65
Identity = 123/145 (84.83%), Postives = 130/145 (89.66%), Query Frame = 1 

Query: 1   MANPTKSLVFVLFFHLFIQISSAGNYNVITFGAKPDGKTDSTQSFLKAWMSACSSLTRST 60
           MANPTKSLVF++FFHLFIQIS+A NYNVI+FGAKPDGKTDSTQSFLKAW SACSS TRST
Sbjct: 1   MANPTKSLVFIVFFHLFIQISTAANYNVISFGAKPDGKTDSTQSFLKAWTSACSSSTRST 60

Query: 61  INVPKGRFLLTSITFRGPCKNSNITFQLNGTLVAPLDYNALGDSRYWILFTKVNGISFIG 120
           INVPKGRFLLT ITFRGPCKN  ITF LNGTLVAPLDY ALGDS YWILF KV+G+SF+G
Sbjct: 61  INVPKGRFLLTPITFRGPCKN-KITFLLNGTLVAPLDYRALGDSGYWILFIKVDGVSFVG 120

Query: 121 GNIDGKGTDYWACKGSGKKCPPGAR 146
           G IDGKGT YWACK S K CPPGAR
Sbjct: 121 GTIDGKGTAYWACKNSAKNCPPGAR 144

[Close]
BLAST of Csa4G153300 vs. TrEMBL
Match: A0A0A0KQL2_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G472110 PE=3 SV=1)

HSP 1 Score: 185.3 bits (469), Expect = 6.0e-44
Identity = 95/145 (65.52%), Postives = 110/145 (75.86%), Query Frame = 1 

Query: 1   MAN-PTKSLVF-VLFFHLFIQISSAGNYNVITFGAKPDGKTDSTQSFLKAWMSACSSLTR 60
           MAN P  S+ F +L   LF Q SSAGNYNVI+FGAK D +TDST SFLKAW SAC+S   
Sbjct: 1   MANFPFFSIHFCLLLITLFFQASSAGNYNVISFGAKGDERTDSTVSFLKAWASACTSPKP 60

Query: 61  STINVPKGRFLLTSITFRGPCKNSNITFQLNGTLVAPLDYNALGDSRYWILFTKVNGISF 120
           STI VPKGRFL+  I F+GPCKN  ITFQ++GT+VAP DY ALG+S  WILF+KVN +S 
Sbjct: 61  STIYVPKGRFLIKGIVFKGPCKN-QITFQIDGTVVAPSDYRALGNSDSWILFSKVNKVSV 120

Query: 121 IGGNIDGKGTDYWACKGSGKKCPPG 144
           +GG +DGKG  YWACK SGK CP G
Sbjct: 121 VGGTLDGKGASYWACKTSGKNCPVG 144

[Close]
BLAST of Csa4G153300 vs. TrEMBL
Match: B9SSX9_RICCO (Polygalacturonase, putative OS=Ricinus communis GN=RCOM_1268300 PE=3 SV=1)

HSP 1 Score: 184.9 bits (468), Expect = 7.8e-44
Identity = 90/145 (62.07%), Postives = 113/145 (77.93%), Query Frame = 1 

Query: 1   MANPTKSLVFVLFFHLFIQISSAGNYNVITFGAKPDGKTDSTQSFLKAWMSACSSLTRST 60
           MAN   +++  L F LF Q+S+A +YNV+ FGAKPDGKTDSTQ FLKAW +ACSS T ST
Sbjct: 1   MANKI-AIMLALSFFLF-QLSNAASYNVLKFGAKPDGKTDSTQPFLKAWAAACSSATAST 60

Query: 61  INVPKGRFLLTSITFRGPCKNSNITFQLNGTLVAPLDYNALGDSRYWILFTKVNGISFIG 120
           I+VPKGR+L+ +I FRGPCK S IT +++GT+ AP+DY ALG+S YWILF +VN IS +G
Sbjct: 61  ISVPKGRYLIKAIEFRGPCK-SRITVKIDGTIEAPVDYRALGNSGYWILFIQVNQISVLG 120

Query: 121 GNIDGKGTDYWACKGSGKKCPPGAR 146
           G +D KG  +WAC+ SGK CP GAR
Sbjct: 121 GTLDAKGAGFWACRASGKSCPVGAR 142

[Close]
BLAST of Csa4G153300 vs. TrEMBL
Match: A0A067E8S7_CITSI (Uncharacterized protein OS=Citrus sinensis GN=CISIN_1g037377mg PE=3 SV=1)

HSP 1 Score: 183.3 bits (464), Expect = 2.3e-43
Identity = 84/139 (60.43%), Postives = 108/139 (77.70%), Query Frame = 1 

Query: 6   KSLVFVLFFHLFIQISSAGNYNVITFGAKPDGKTDSTQSFLKAWMSACSSLTRSTINVPK 65
           K ++F+LF  +FI+ S+A NYNVIT+GAKPDG+TDST+ FLKAW SAC S   STI VPK
Sbjct: 4   KLIIFLLFVIIFIKSSNAANYNVITYGAKPDGRTDSTKPFLKAWASACRSAKASTIYVPK 63

Query: 66  GRFLLTSITFRGPCKNSNITFQLNGTLVAPLDYNALGDSRYWILFTKVNGISFIGGNIDG 125
           GR+L+ +  FRGPC+N  +T Q++GT+VAP +Y ALG+S YWILF K++ +S IGG  DG
Sbjct: 64  GRYLIKAAEFRGPCRN-RVTLQIDGTIVAPTNYWALGNSGYWILFIKIDRLSVIGGTFDG 123

Query: 126 KGTDYWACKGSGKKCPPGA 145
           KG  +WAC+ SGK CP GA
Sbjct: 124 KGAGFWACRKSGKNCPAGA 141

[Close]
BLAST of Csa4G153300 vs. TAIR10
Match: AT2G43890.1 (AT2G43890.1 Pectin lyase-like superfamily protein)

HSP 1 Score: 174.1 bits (440), Expect = 7.0e-44
Identity = 83/148 (56.08%), Postives = 102/148 (68.92%), Query Frame = 1 

Query: 1   MANPTKSLVFVLFFHLFIQISS---AGNYNVITFGAKPDGKTDSTQSFLKAWMSACSSLT 60
           M N     V ++FF  F+ + S   A NYNV++FGAKPDG+TDST++FL AW +AC S  
Sbjct: 1   MDNNKLVAVLLMFFSSFLLMKSSTAASNYNVVSFGAKPDGRTDSTKAFLGAWQAACRSAA 60

Query: 61  RSTINVPKGRFLLTSITFRGPCKNSNITFQLNGTLVAPLDYNALGDSRYWILFTKVNGIS 120
             T+ VP+G FLL  + FRGPC+ S ITFQ+ GT+VAP DY  LG+S YWILF KVN IS
Sbjct: 61  AVTVTVPRGSFLLKPVEFRGPCR-SRITFQIYGTIVAPSDYRGLGNSGYWILFVKVNRIS 120

Query: 121 FIGGNIDGKGTDYWACKGSGKKCPPGAR 146
            IGG +D +G  +WAC+ SGK CP GAR
Sbjct: 121 IIGGTLDARGASFWACRKSGKSCPVGAR 147

[Close]
BLAST of Csa4G153300 vs. TAIR10
Match: AT1G05660.1 (AT1G05660.1 Pectin lyase-like superfamily protein)

HSP 1 Score: 163.7 bits (413), Expect = 9.4e-41
Identity = 75/139 (53.96%), Postives = 96/139 (69.06%), Query Frame = 1 

Query: 7   SLVFVLFFHLFIQISSAGNYNVITFGAKPDGKTDSTQSFLKAWMSACSSLTRSTINVPKG 66
           SL+F L   + + IS++  +NV++FGAKPDG TDST +FLKAW  AC S + +T+ VPKG
Sbjct: 9   SLLFTLLTFIDVSISASNVFNVVSFGAKPDGVTDSTGAFLKAWQGACVSASSATVVVPKG 68

Query: 67  RFLLTSITFRGPCKNSNITFQLNGTLVAPLDYNALGDSRYWILFTKVNGISFIGGNIDGK 126
            FLL  ITF GPCK S ITFQ+ GT++AP DY   G+S +WILF KVN  S +GG  D +
Sbjct: 69  TFLLKVITFGGPCK-SKITFQVAGTVIAPEDYRTFGNSGFWILFNKVNRFSLVGGTFDAR 128

Query: 127 GTDYWACKGSGKKCPPGAR 146
              +W+C+ SG+ CPPG R
Sbjct: 129 ANGFWSCRKSGQNCPPGVR 146

[Close]
BLAST of Csa4G153300 vs. TAIR10
Match: AT1G05650.1 (AT1G05650.1 Pectin lyase-like superfamily protein)

HSP 1 Score: 161.0 bits (406), Expect = 6.1e-40
Identity = 81/146 (55.48%), Postives = 99/146 (67.81%), Query Frame = 1 

Query: 5   TKSLV-FVLFFHL--FIQISSAGN--YNVITFGAKPDGKTDSTQSFLKAWMSACSSLTRS 64
           TKS + F L F L  FI +SS+ +  +NV++FGAKPDG TDST +FLKAW  AC S   +
Sbjct: 2   TKSAITFPLIFTLLTFIDVSSSASIVFNVVSFGAKPDGVTDSTAAFLKAWQGACGSAASA 61

Query: 65  TINVPKGRFLLTSITFRGPCKNSNITFQLNGTLVAPLDYNALGDSRYWILFTKVNGISFI 124
           T+ VP G FLL  ITF GPCK S ITFQ+ GT+VAP DY   G+S  WILF KVN  S +
Sbjct: 62  TVVVPTGTFLLKVITFGGPCK-SKITFQVTGTVVAPEDYRTFGNSGSWILFNKVNRFSLV 121

Query: 125 GGNIDGKGTDYWACKGSGKKCPPGAR 146
           GG  D +G+ +W+C+ SG+ CPPG R
Sbjct: 122 GGTFDARGSGFWSCRKSGQNCPPGVR 146

[Close]
BLAST of Csa4G153300 vs. TAIR10
Match: AT3G59850.1 (AT3G59850.1 Pectin lyase-like superfamily protein)

HSP 1 Score: 159.8 bits (403), Expect = 1.4e-39
Identity = 76/139 (54.68%), Postives = 94/139 (67.63%), Query Frame = 1 

Query: 7   SLVFVLFFHLFIQISSAGNYNVITFGAKPDGKTDSTQSFLKAWMSACSSLTRSTINVPKG 66
           SL+ VL F L +  SSA  YN++++GAKPDGKTDST++F   W  AC+S+   TI VPKG
Sbjct: 4   SLLIVLLFLLSVSSSSAQTYNILSYGAKPDGKTDSTKAFTVLWAKACASVKPVTILVPKG 63

Query: 67  RFLLTSITFRGP-CKNSNITFQLNGTLVAPLDYNALGDSRYWILFTKVNGISFIGGNIDG 126
           RFLL SI F G  CK  ++TF++ GTLVAP DY  +G   YWILF  ++GIS  GG +D 
Sbjct: 64  RFLLRSIIFDGSKCKRKSVTFRIQGTLVAPSDYRVIGKENYWILFQHLDGISVYGGVLDA 123

Query: 127 KGTDYWACKGSGKKCPPGA 145
           +G   W+CK SGK CP GA
Sbjct: 124 QGASLWSCKKSGKNCPSGA 142

[Close]
BLAST of Csa4G153300 vs. TAIR10
Match: AT2G43870.1 (AT2G43870.1 Pectin lyase-like superfamily protein)

HSP 1 Score: 156.8 bits (395), Expect = 1.2e-38
Identity = 73/137 (53.28%), Postives = 93/137 (67.88%), Query Frame = 1 

Query: 9   VFVLFFHLFIQISSAGNYNVITFGAKPDGKTDSTQSFLKAWMSACSSLTRSTINVPKGRF 68
           + VLF   FI   SA +YNV++FGAKPDGKTD+T++F+  W +AC+S    TI VPKGRF
Sbjct: 4   LLVLFVFFFISSCSAQSYNVLSFGAKPDGKTDATKAFMAVWQTACASSRPVTIVVPKGRF 63

Query: 69  LLTSITFRG-PCKNSNITFQLNGTLVAPLDYNALGDSRYWILFTKVNGISFIGGNIDGKG 128
           LL S+TF G  CK   +TF+++GTLVAP DY  +G+  YWI F  ++GI+  GG +D +G
Sbjct: 64  LLRSVTFDGSKCKPKPVTFRIDGTLVAPADYRVIGNEDYWIFFQHLDGITVYGGVLDARG 123

Query: 129 TDYWACKGSGKKCPPGA 145
              W CK SGK CP GA
Sbjct: 124 ASLWDCKKSGKNCPSGA 140

[Close]
BLAST of Csa4G153300 vs. NCBI nr
Match: gi|700198669|gb|KGN53827.1| (hypothetical protein Csa_4G153300 [Cucumis sativus])

HSP 1 Score: 335.5 bits (859), Expect = 5.1e-89
Identity = 160/160 (100.00%), Postives = 160/160 (100.00%), Query Frame = 1 

Query: 1   MANPTKSLVFVLFFHLFIQISSAGNYNVITFGAKPDGKTDSTQSFLKAWMSACSSLTRST 60
           MANPTKSLVFVLFFHLFIQISSAGNYNVITFGAKPDGKTDSTQSFLKAWMSACSSLTRST
Sbjct: 1   MANPTKSLVFVLFFHLFIQISSAGNYNVITFGAKPDGKTDSTQSFLKAWMSACSSLTRST 60

Query: 61  INVPKGRFLLTSITFRGPCKNSNITFQLNGTLVAPLDYNALGDSRYWILFTKVNGISFIG 120
           INVPKGRFLLTSITFRGPCKNSNITFQLNGTLVAPLDYNALGDSRYWILFTKVNGISFIG
Sbjct: 61  INVPKGRFLLTSITFRGPCKNSNITFQLNGTLVAPLDYNALGDSRYWILFTKVNGISFIG 120

Query: 121 GNIDGKGTDYWACKGSGKKCPPGARKAEKKHQKYYFLPKN 161
           GNIDGKGTDYWACKGSGKKCPPGARKAEKKHQKYYFLPKN
Sbjct: 121 GNIDGKGTDYWACKGSGKKCPPGARKAEKKHQKYYFLPKN 160

[Close]
BLAST of Csa4G153300 vs. NCBI nr
Match: gi|659121741|ref|XP_008460791.1| (PREDICTED: polygalacturonase-like [Cucumis melo])

HSP 1 Score: 278.5 bits (711), Expect = 7.5e-72
Identity = 132/145 (91.03%), Postives = 138/145 (95.17%), Query Frame = 1 

Query: 1   MANPTKSLVFVLFFHLFIQISSAGNYNVITFGAKPDGKTDSTQSFLKAWMSACSSLTRST 60
           MAN  KSLVF++FFHLFIQISSAG+YNVITFGAKPDGKTDSTQSFLKAW SACSSLTRST
Sbjct: 1   MANSRKSLVFIVFFHLFIQISSAGSYNVITFGAKPDGKTDSTQSFLKAWKSACSSLTRST 60

Query: 61  INVPKGRFLLTSITFRGPCKNSNITFQLNGTLVAPLDYNALGDSRYWILFTKVNGISFIG 120
           INVPKGRFLLT+ITFRGPCKN+NI F LNGTLVAPLDYNALGDS YWILFTKVNG+SFIG
Sbjct: 61  INVPKGRFLLTAITFRGPCKNNNIIFLLNGTLVAPLDYNALGDSGYWILFTKVNGVSFIG 120

Query: 121 GNIDGKGTDYWACKGSGKKCPPGAR 146
           GNIDGKGT YWACKGSGKKCPPGAR
Sbjct: 121 GNIDGKGTAYWACKGSGKKCPPGAR 145

[Close]
BLAST of Csa4G153300 vs. NCBI nr
Match: gi|778665426|ref|XP_004142576.2| (PREDICTED: polygalacturonase-like [Cucumis sativus])

HSP 1 Score: 256.9 bits (655), Expect = 2.3e-65
Identity = 123/145 (84.83%), Postives = 130/145 (89.66%), Query Frame = 1 

Query: 1   MANPTKSLVFVLFFHLFIQISSAGNYNVITFGAKPDGKTDSTQSFLKAWMSACSSLTRST 60
           MANPTKSLVF++FFHLFIQIS+A NYNVI+FGAKPDGKTDSTQSFLKAW SACSS TRST
Sbjct: 1   MANPTKSLVFIVFFHLFIQISTAANYNVISFGAKPDGKTDSTQSFLKAWTSACSSSTRST 60

Query: 61  INVPKGRFLLTSITFRGPCKNSNITFQLNGTLVAPLDYNALGDSRYWILFTKVNGISFIG 120
           INVPKGRFLLT ITFRGPCKN  ITF LNGTLVAPLDY ALGDS YWILF KV+G+SF+G
Sbjct: 61  INVPKGRFLLTPITFRGPCKN-KITFLLNGTLVAPLDYRALGDSGYWILFIKVDGVSFVG 120

Query: 121 GNIDGKGTDYWACKGSGKKCPPGAR 146
           G IDGKGT YWACK S K CPPGAR
Sbjct: 121 GTIDGKGTAYWACKNSAKNCPPGAR 144

[Close]
BLAST of Csa4G153300 vs. NCBI nr
Match: gi|449452150|ref|XP_004143823.1| (PREDICTED: polygalacturonase-like [Cucumis sativus])

HSP 1 Score: 185.3 bits (469), Expect = 8.6e-44
Identity = 95/145 (65.52%), Postives = 110/145 (75.86%), Query Frame = 1 

Query: 1   MAN-PTKSLVF-VLFFHLFIQISSAGNYNVITFGAKPDGKTDSTQSFLKAWMSACSSLTR 60
           MAN P  S+ F +L   LF Q SSAGNYNVI+FGAK D +TDST SFLKAW SAC+S   
Sbjct: 1   MANFPFFSIHFCLLLITLFFQASSAGNYNVISFGAKGDERTDSTVSFLKAWASACTSPKP 60

Query: 61  STINVPKGRFLLTSITFRGPCKNSNITFQLNGTLVAPLDYNALGDSRYWILFTKVNGISF 120
           STI VPKGRFL+  I F+GPCKN  ITFQ++GT+VAP DY ALG+S  WILF+KVN +S 
Sbjct: 61  STIYVPKGRFLIKGIVFKGPCKN-QITFQIDGTVVAPSDYRALGNSDSWILFSKVNKVSV 120

Query: 121 IGGNIDGKGTDYWACKGSGKKCPPG 144
           +GG +DGKG  YWACK SGK CP G
Sbjct: 121 VGGTLDGKGASYWACKTSGKNCPVG 144

[Close]
BLAST of Csa4G153300 vs. NCBI nr
Match: gi|700195997|gb|KGN51174.1| (hypothetical protein Csa_5G472110 [Cucumis sativus])

HSP 1 Score: 185.3 bits (469), Expect = 8.6e-44
Identity = 95/145 (65.52%), Postives = 110/145 (75.86%), Query Frame = 1 

Query: 1   MAN-PTKSLVF-VLFFHLFIQISSAGNYNVITFGAKPDGKTDSTQSFLKAWMSACSSLTR 60
           MAN P  S+ F +L   LF Q SSAGNYNVI+FGAK D +TDST SFLKAW SAC+S   
Sbjct: 1   MANFPFFSIHFCLLLITLFFQASSAGNYNVISFGAKGDERTDSTVSFLKAWASACTSPKP 60

Query: 61  STINVPKGRFLLTSITFRGPCKNSNITFQLNGTLVAPLDYNALGDSRYWILFTKVNGISF 120
           STI VPKGRFL+  I F+GPCKN  ITFQ++GT+VAP DY ALG+S  WILF+KVN +S 
Sbjct: 61  STIYVPKGRFLIKGIVFKGPCKN-QITFQIDGTVVAPSDYRALGNSDSWILFSKVNKVSV 120

Query: 121 IGGNIDGKGTDYWACKGSGKKCPPG 144
           +GG +DGKG  YWACK SGK CP G
Sbjct: 121 VGGTLDGKGASYWACKTSGKNCPVG 144

The following BLAST results are available for this feature:
BLAST of Csa4G153300 vs. Swiss-Prot
Match NameE-valueIdentityDescription
PGLR_PRUPE1.6e-3451.80Polygalacturonase OS=Prunus persica PE=2 SV=1[more]
PGLR6_ARATH1.2e-2646.28Probable polygalacturonase At2g43860 OS=Arabidopsis thaliana GN=At2g43860 PE=2 S... [more]
PGLR3_ARATH1.1e-1941.79Probable polygalacturonase At3g15720 OS=Arabidopsis thaliana GN=At3g15720 PE=1 S... [more]
ADPG1_ARATH2.8e-1844.92Polygalacturonase ADPG1 OS=Arabidopsis thaliana GN=ADPG1 PE=2 SV=1[more]
PGLR1_MAIZE8.1e-1838.46Exopolygalacturonase OS=Zea mays GN=PG1 PE=1 SV=1[more]
BLAST of Csa4G153300 vs. TrEMBL
Match NameE-valueIdentityDescription
A0A0A0KZM5_CUCSA3.6e-89100.00Uncharacterized protein OS=Cucumis sativus GN=Csa_4G153300 PE=3 SV=1[more]
A0A0A0M0F3_CUCSA1.6e-6584.83Uncharacterized protein OS=Cucumis sativus GN=Csa_1G689640 PE=3 SV=1[more]
A0A0A0KQL2_CUCSA6.0e-4465.52Uncharacterized protein OS=Cucumis sativus GN=Csa_5G472110 PE=3 SV=1[more]
B9SSX9_RICCO7.8e-4462.07Polygalacturonase, putative OS=Ricinus communis GN=RCOM_1268300 PE=3 SV=1[more]
A0A067E8S7_CITSI2.3e-4360.43Uncharacterized protein OS=Citrus sinensis GN=CISIN_1g037377mg PE=3 SV=1[more]
BLAST of Csa4G153300 vs. TAIR10
Match NameE-valueIdentityDescription
AT2G43890.17.0e-4456.08 Pectin lyase-like superfamily protein[more]
AT1G05660.19.4e-4153.96 Pectin lyase-like superfamily protein[more]
AT1G05650.16.1e-4055.48 Pectin lyase-like superfamily protein[more]
AT3G59850.11.4e-3954.68 Pectin lyase-like superfamily protein[more]
AT2G43870.11.2e-3853.28 Pectin lyase-like superfamily protein[more]
BLAST of Csa4G153300 vs. NCBI nr
Match NameE-valueIdentityDescription
gi|700198669|gb|KGN53827.1|5.1e-89100.00hypothetical protein Csa_4G153300 [Cucumis sativus][more]
gi|659121741|ref|XP_008460791.1|7.5e-7291.03PREDICTED: polygalacturonase-like [Cucumis melo][more]
gi|778665426|ref|XP_004142576.2|2.3e-6584.83PREDICTED: polygalacturonase-like [Cucumis sativus][more]
gi|449452150|ref|XP_004143823.1|8.6e-4465.52PREDICTED: polygalacturonase-like [Cucumis sativus][more]
gi|700195997|gb|KGN51174.1|8.6e-4465.52hypothetical protein Csa_5G472110 [Cucumis sativus][more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR000743Glyco_hydro_28
IPR011050Pectin_lyase_fold/virulence
IPR012334Pectin_lyas_fold
Vocabulary: Molecular Function
TermDefinition
GO:0004650polygalacturonase activity
Vocabulary: Biological Process
TermDefinition
GO:0005975carbohydrate metabolic process
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0071555 cell wall organization
biological_process GO:0005982 starch metabolic process
biological_process GO:0005985 sucrose metabolic process
biological_process GO:0005975 carbohydrate metabolic process
cellular_component GO:0005576 extracellular region
cellular_component GO:0005575 cellular_component
molecular_function GO:0004650 polygalacturonase activity
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Csa4G153300.1Csa4G153300.1mRNA


InterPro
Analysis Name: InterPro Annotations of cucumber (Chinese Long)
Date Performed: 2016-09-28
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000743Glycoside hydrolase, family 28PFAMPF00295Glyco_hydro_28coord: 53..139
score: 6.5
IPR011050Pectin lyase fold/virulence factorunknownSSF51126Pectin lyase-likecoord: 8..132
score: 5.02
IPR012334Pectin lyase foldGENE3DG3DSA:2.160.20.10coord: 11..139
score: 2.9
NoneNo IPR availablePANTHERPTHR31375FAMILY NOT NAMEDcoord: 1..145
score: 9.4
NoneNo IPR availablePANTHERPTHR31375:SF31F3F20.10 PROTEIN-RELATEDcoord: 1..145
score: 9.4

Synteny
The following gene(s) are orthologous to this gene:
GeneOrthologueOrganismBlock
Csa4G153300MELO3C023170Melon (DHL92) v3.5.1cumeB331
Csa4G153300MELO3C023170.2Melon (DHL92) v3.6.1cumedB313
Csa4G153300CSPI04G10420Wild cucumber (PI 183967)cpicuB182
Csa4G153300Cucsa.038070Cucumber (Gy14) v1cgycuB011
Csa4G153300CsGy4G010110Cucumber (Gy14) v2cgybcuB165
The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:
GeneOrganismBlock
Csa4G153300Cucurbita maxima (Rimu)cmacuB673
Csa4G153300Cucurbita moschata (Rifu)cmocuB662
Csa4G153300Watermelon (Charleston Gray)cuwcgB305
Csa4G153300Cucurbita pepo (Zucchini)cpecuB074
Csa4G153300Bottle gourd (USVL1VR-Ls)culsiB260
Csa4G153300Silver-seed gourdcarcuB0214
Csa4G153300Watermelon (97103) v2cuwmbB302
Csa4G153300Wax gourdcuwgoB392