Cucsa.032120 (gene) Cucumber (Gy14) v1

NameCucsa.032120
Typegene
OrganismCucumis sativus (Cucumber (Gy14) v1)
DescriptionPentatricopeptide repeat-containing protein
Locationscaffold00429 : 1537293 .. 1540244 (+)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCATTATCGAAGCCGGCCTTCTTCACCCACCTCAAAACCCTAACCGGTTCCCACCATTTACTCCAGCGCCAAGCTCTGGCACCATTTCCTATCGTCACTCTCCGATTTCTCTCTTTTGCATCGGCTGAGGAAGCTGATGCCGAACGACGCCGTCGTAAGCGCCGCCTCCGCATCGAACCCCCGCTCTCTTCTTCCTCTGCCGCTCGTCCACTAACGCAGCCTCCTAGATCTCAAACCCCACAAAACCCTAATGCCCCGAAAATTCCTGAGCACATCTCTGCTCTCTCTGGTAATCGTCTTAACCTCCACAATCGCATTCTCACTCTCATTCGTGAAAATGATCTTGAAGAAGCCGCGCTTTTCACTCGTCATTCAATTTATTCCAATTGTCGTCCCACCATCTTCACCGTCAATGCGGTTCTCAATGCACAGCTTCGTCAATCGAAATATGCCGATTTGCTTTCACTTCACCGGTTTATTACACAGGCTGGTGTCGTCCCTAATATAATCACTCACAATTTGATTTTTCAGACTTATTTGGATTGCCGTAAGCCTGATACGGCAATGGAACATTACAAGCAGTTGATCAATGATGCACCTTTCAACCCCTCGCCGACGACTTATAGGATCTTGATTAAGGGGTTGGTAGACAATAACAAGTTGGAGAGGGCAATGGAGCTTAAAGACGAAATGATTGAGAAAGGTTTTGCTCCTGACCCTCTTATTTATCATTATCTGATGGGGGGATGTGTGAGAAGTTTGGATCCTGATGGAGTTTTTAAGCTTTTTGAAGAGTTGAAAGAAAAGTTGGGAGCGACTGTGGAAGATGGAGTTGTTTATGGGAACTTGATGAAAGGGTATTTTATGAAAGAAATGGAAGAGGAAGCAATGAAGTGTTATGAGGAGACTGTGGGTGATAATTCAGTGGTGAAGATGAGTGCCATTGCATACAATTCTGTGCTTGATGCATTATGCAGGAATGGGAAATTTGGTGAGGCTTTGACATTGTTTGATAGGATGACAAAAGAGCATCGTCCGCCCAGGCATCTGGCAGTGAACTTGGGAAGCTTTAATGTTATGGTTGATGGGTACTGCATCGAAGGGAGGTTCAAAGAGGCCATTGAAGTATTTGAGAAGATGGGTGATTATAGGTGTTGCCCAGATACGTTATCATTCAATAATTTGATCGAACAATTATGTAATAATGGAATGTTGGCTGAAGCTGAGATGCTTTACGGAACAATGGATGATAAGGGAGTAAACCCGGACGAGTTTACTTATGGTTTGTTGATGGATTCTTGCTTTAAAAAAAaCAGGGCAGATGATGCAGCTGCATATTTTAGAAAAaTGGTAGATTCTGGACTCAGACCCAATATAGCAGTTTATAATATATTAGTGGACGAGTTGGTTAAATTAGGGAAGATCGACGACGCAAAGTCTTTCTTTGATTTGATGGTGAAGAAGCTCAAAATGGATGCCTCGAGCTATCAGTTTATAATGAAAGCGTTGAGTGAATCAGGAAAAATGGACGAAATACTAAATGTGGTCGATACCCTTCTTGATGATGATGGGATTGAATTTTCGGAAGAGCTGCAGGAGTTCGTGAGAGGTGAGTTGAGGAAGGAAAACAGGGAAGAAGATCTAGCTAAACTTGTGGAAGAGAAAGAAAGACTGAAAGCTGAAGCTAAGGCAAAGGAGGCTGAAGCAGCAGAGGCACAGAAGAGAAGTGCTAAAGCTGCTGTTTCTTCTTTACTGTCATCCAAGTTGTTTGCGAACAAGGAAGGTGAGAAAGAATCTGTAGTGAATGAAATGCAATCTGTTGAACAAGAAGACGACAGTGGTAAAACTGAACTTGCGGAATCAAGTCCTTGAAGAAATTGCGTAGTAGAGGGGAAAGTACTGAAGAGCGAGAATTAAACTGTTTAGACATGGAAGAACAGGAGAATTAAACTGTTTAGACATGGAAGAACAGGTGAAATTCACATCAGATTTTGTGTTAGTTGTTGCTTCGATTTGCATGGGAATGTGATCTGTTTTCCATTTCTGTTAACTTTAAATTATTCTCGTTGTTACATTACATGGAAATGTGATCCGCTCTTGGTTCTCTTAACCAATTAGGCAAGAGAAATCTGTACGACAAAAAGAAAATTGATAGACTTCTTTACCTTGCATTTTTGCTTATAATGTTAATGTAATCAGTAATTGTTTCCACTTCTATTTTTTTTtAGTAGATTCAAACGACGGACATACTTAATGAGTACCGATTGAGTAGACAAAACATCCGTAAACTAAACCAATGTGCAATCATTCATGTGTTCCTAGATTAACCCCTTGTACTTTACTCCTGAACTTCCAAGCAAGGATACACGAAAATCCACTTATAAGTTGGTGAATGTTACAATTTTGGCACCATTGTTGATATGAAGATCAAGAATTCTCTCAAGTATATACAATTAAAAAATATCACCCGTCACAATATATGAGAATAGGTACAAAGCAGAGTACTGTCAAAAACACACAGAAAGGCAGGCATTAAGTAAACACTTCGATTTTAGCTACAAAGGTTTGGTATGCAAACACCAGCATCCGGGTTTTGTTCCTTCCATATCCTTCCTGTAACCCTCAGCTTCAGTTCCTTTCTGTCCCCACCAGAGAAGAAAAGAGCCATATTTCTTTGGATAATTAATCCATCATGGCTGTCCCTAACTTTTCGATGACTATCTCTAATACTTCGTGGTGTACCCTCCCATATCAGTTTTCGCCCATATCCACCAACCTCGAGACTGTAGCTGTAGGTTCGGGCGTCATTCTCATCGCCCATGAAACGGAGGAAAGCCATGTAGACTGGAGCCATGCCGAGCTGAAAAGCCTCAAAGTGAAGGCAGAAATATTGACCAAAACAATGAAAAACCTGCAAAATGTGAGAAGCGCAAGCCACAAGACATGTGA

mRNA sequence

ATGGCATTATCGAAGCCGGCCTTCTTCACCCACCTCAAAACCCTAACCGCTGATGCCGAACGACGCCGTCGTAAGCGCCGCCTCCGCATCGAACCCCCGCTCTCTTCTTCCTCTGCCGCTCGTCCACTAACGCAGCCTCCTAGATCTCAAACCCCACAAAACCCTAATGCCCCGAAAATTCCTGAGCACATCTCTGCTCTCTCTGGTAATCGTCTTAACCTCCACAATCGCATTCTCACTCTCATTCGTGAAAATGATCTTGAAGAAGCCGCGCTTTTCACTCGTCATTCAATTTATTCCAATTGTCGTCCCACCATCTTCACCGTCAATGCGGTTCTCAATGCACAGCTTCGTCAATCGAAATATGCCGATTTGCTTTCACTTCACCGGTTTATTACACAGGCTGGTGTCGTCCCTAATATAATCACTCACAATTTGATTTTTCAGACTTATTTGGATTGCCGTAAGCCTGATACGGCAATGGAACATTACAAGCAGTTGATCAATGATGCACCTTTCAACCCCTCGCCGACGACTTATAGGATCTTGATTAAGGGGTTGGTAGACAATAACAAGTTGGAGAGGGCAATGGAGCTTAAAGACGAAATGATTGAGAAAGGTTTTGCTCCTGACCCTCTTATTTATCATTATCTGATGGGGGGATGTGTGAGAAGTTTGGATCCTGATGGAGTTTTTAAGCTTTTTGAAGAGTTGAAAGAAAAGTTGGGAGCGACTGTGGAAGATGGAGTTGTTTATGGGAACTTGATGAAAGGGTATTTTATGAAAGAAATGGAAGAGGAAGCAATGAAGTGTTATGAGGAGACTGTGGGTGATAATTCAGTGGTGAAGATGAGTGCCATTGCATACAATTCTGTGCTTGATGCATTATGCAGGAATGGGAAATTTGGTGAGGCTTTGACATTGTTTGATAGGATGACAAAAGAGCATCGTCCGCCCAGGCATCTGGCAGTGAACTTGGGAAGCTTTAATGTTATGGTTGATGGGTACTGCATCGAAGGGAGGTTCAAAGAGGCCATTGAAGTATTTGAGAAGATGGGTGATTATAGGTGTTGCCCAGATACGTTATCATTCAATAATTTGATCGAACAATTATGTAATAATGGAATGTTGGCTGAAGCTGAGATGCTTTACGGAACAATGGATGATAAGGGAGTAAACCCGGACGAGTTTACTTATGGTTTGTTGATGGATTCTTGCTTTAAAAAAAACAGGGCAGATGATGCAGCTGCATATTTTAGAAAAATGGTAGATTCTGGACTCAGACCCAATATAGCAGTTTATAATATATTAGTGGACGAGTTGGTTAAATTAGGGAAGATCGACGACGCAAAGTCTTTCTTTGATTTGATGGTGAAGAAGCTCAAAATGGATGCCTCGAGCTATCAGTTTATAATGAAAGCGTTGAGTGAATCAGGAAAAATGGACGAAATACTAAATGTGGTCGATACCCTTCTTGATGATGATGGGATTGAATTTTCGGAAGAGCTGCAGGAGTTCGTGAGAGGTGAGTTGAGGAAGGAAAACAGGGAAGAAGATCTAGCTAAACTTGTGGAAGAGAAAGAAAGACTGAAAGCTGAAGCTAAGGCAAAGGAGGCTGAAGCAGCAGAGGCACAGAAGAGAAGTGCTAAAGCTGCTGTTTCTTCTTTACTGTCATCCAAGTTGTTTGCGAACAAGGAAGGTGAGAAAGAATCTGTACCATGTAGACTGGAGCCATGCCGAGCTGAAAAGCCTCAAAGTGAAGGCAGAAATATTGACCAAAACAATGAAAAACCTGCAAAATGTGAGAAGCGCAAGCCACAAGACATGTGA

Coding sequence (CDS)

ATGGCATTATCGAAGCCGGCCTTCTTCACCCACCTCAAAACCCTAACCGCTGATGCCGAACGACGCCGTCGTAAGCGCCGCCTCCGCATCGAACCCCCGCTCTCTTCTTCCTCTGCCGCTCGTCCACTAACGCAGCCTCCTAGATCTCAAACCCCACAAAACCCTAATGCCCCGAAAATTCCTGAGCACATCTCTGCTCTCTCTGGTAATCGTCTTAACCTCCACAATCGCATTCTCACTCTCATTCGTGAAAATGATCTTGAAGAAGCCGCGCTTTTCACTCGTCATTCAATTTATTCCAATTGTCGTCCCACCATCTTCACCGTCAATGCGGTTCTCAATGCACAGCTTCGTCAATCGAAATATGCCGATTTGCTTTCACTTCACCGGTTTATTACACAGGCTGGTGTCGTCCCTAATATAATCACTCACAATTTGATTTTTCAGACTTATTTGGATTGCCGTAAGCCTGATACGGCAATGGAACATTACAAGCAGTTGATCAATGATGCACCTTTCAACCCCTCGCCGACGACTTATAGGATCTTGATTAAGGGGTTGGTAGACAATAACAAGTTGGAGAGGGCAATGGAGCTTAAAGACGAAATGATTGAGAAAGGTTTTGCTCCTGACCCTCTTATTTATCATTATCTGATGGGGGGATGTGTGAGAAGTTTGGATCCTGATGGAGTTTTTAAGCTTTTTGAAGAGTTGAAAGAAAAGTTGGGAGCGACTGTGGAAGATGGAGTTGTTTATGGGAACTTGATGAAAGGGTATTTTATGAAAGAAATGGAAGAGGAAGCAATGAAGTGTTATGAGGAGACTGTGGGTGATAATTCAGTGGTGAAGATGAGTGCCATTGCATACAATTCTGTGCTTGATGCATTATGCAGGAATGGGAAATTTGGTGAGGCTTTGACATTGTTTGATAGGATGACAAAAGAGCATCGTCCGCCCAGGCATCTGGCAGTGAACTTGGGAAGCTTTAATGTTATGGTTGATGGGTACTGCATCGAAGGGAGGTTCAAAGAGGCCATTGAAGTATTTGAGAAGATGGGTGATTATAGGTGTTGCCCAGATACGTTATCATTCAATAATTTGATCGAACAATTATGTAATAATGGAATGTTGGCTGAAGCTGAGATGCTTTACGGAACAATGGATGATAAGGGAGTAAACCCGGACGAGTTTACTTATGGTTTGTTGATGGATTCTTGCTTTAAAAAAAaCAGGGCAGATGATGCAGCTGCATATTTTAGAAAAaTGGTAGATTCTGGACTCAGACCCAATATAGCAGTTTATAATATATTAGTGGACGAGTTGGTTAAATTAGGGAAGATCGACGACGCAAAGTCTTTCTTTGATTTGATGGTGAAGAAGCTCAAAATGGATGCCTCGAGCTATCAGTTTATAATGAAAGCGTTGAGTGAATCAGGAAAAATGGACGAAATACTAAATGTGGTCGATACCCTTCTTGATGATGATGGGATTGAATTTTCGGAAGAGCTGCAGGAGTTCGTGAGAGGTGAGTTGAGGAAGGAAAACAGGGAAGAAGATCTAGCTAAACTTGTGGAAGAGAAAGAAAGACTGAAAGCTGAAGCTAAGGCAAAGGAGGCTGAAGCAGCAGAGGCACAGAAGAGAAGTGCTAAAGCTGCTGTTTCTTCTTTACTGTCATCCAAGTTGTTTGCGAACAAGGAAGGTGAGAAAGAATCTGTACCATGTAGACTGGAGCCATGCCGAGCTGAAAAGCCTCAAAGTGAAGGCAGAAATATTGACCAAAACAATGAAAAACCTGCAAAATGTGAGAAGCGCAAGCCACAAGACATGTGA

Protein sequence

MALSKPAFFTHLKTLTADAERRRRKRRLRIEPPLSSSSAARPLTQPPRSQTPQNPNAPKIPEHISALSGNRLNLHNRILTLIRENDLEEAALFTRHSIYSNCRPTIFTVNAVLNAQLRQSKYADLLSLHRFITQAGVVPNIITHNLIFQTYLDCRKPDTAMEHYKQLINDAPFNPSPTTYRILIKGLVDNNKLERAMELKDEMIEKGFAPDPLIYHYLMGGCVRSLDPDGVFKLFEELKEKLGATVEDGVVYGNLMKGYFMKEMEEEAMKCYEETVGDNSVVKMSAIAYNSVLDALCRNGKFGEALTLFDRMTKEHRPPRHLAVNLGSFNVMVDGYCIEGRFKEAIEVFEKMGDYRCCPDTLSFNNLIEQLCNNGMLAEAEMLYGTMDDKGVNPDEFTYGLLMDSCFKKNRADDAAAYFRKMVDSGLRPNIAVYNILVDELVKLGKIDDAKSFFDLMVKKLKMDASSYQFIMKALSESGKMDEILNVVDTLLDDDGIEFSEELQEFVRGELRKENREEDLAKLVEEKERLKAEAKAKEAEAAEAQKRSAKAAVSSLLSSKLFANKEGEKESVPCRLEPCRAEKPQSEGRNIDQNNEKPAKCEKRKPQDM*
BLAST of Cucsa.032120 vs. Swiss-Prot
Match: PP273_ARATH (Pentatricopeptide repeat-containing protein At3g49240 OS=Arabidopsis thaliana GN=EMB1796 PE=2 SV=1)

HSP 1 Score: 748.4 bits (1931), Expect = 6.0e-215
Identity = 373/594 (62.79%), Postives = 462/594 (77.78%), Query Frame = 1

Query: 1   MALSKPAFFTHLKTLT-------------------------ADAERRRRKRRLRIEPPLS 60
           M++SK AF  HL+TL+                         A AERRRRKRRLR+EPP++
Sbjct: 1   MSISKAAFLNHLQTLSRSYRHRVLPQPFLAVRYMSFATQEEAAAERRRRKRRLRMEPPVN 60

Query: 61  SSSAARPLTQPPRSQTP---QNPNAPKIPEHISALSGNRLNLHNRILTLIRENDLEEAAL 120
           S + +    Q  +SQ P   QNPN PK+PE +SAL G RL+LHN IL LIRENDLEEAAL
Sbjct: 61  SFNRS----QQQQSQIPRPIQNPNIPKLPESVSALVGKRLDLHNHILKLIRENDLEEAAL 120

Query: 121 FTRHSIYSNCRPTIFTVNAVLNAQLRQSKYADLLSLHRFITQAGVVPNIITHNLIFQTYL 180
           +TRHS+YSNCRPTIFTVN VL AQLRQ+KY  LL LH FI QAG+ PNIIT+NLIFQ YL
Sbjct: 121 YTRHSVYSNCRPTIFTVNTVLAAQLRQAKYGALLQLHGFINQAGIAPNIITYNLIFQAYL 180

Query: 181 DCRKPDTAMEHYKQLINDAPFNPSPTTYRILIKGLVDNNKLERAMELKDEMIEKGFAPDP 240
           D RKP+ A+EHYK  I++AP NPS  T+RIL+KGLV N+ LE+AME+K++M  KGF  DP
Sbjct: 181 DVRKPEIALEHYKLFIDNAPLNPSIATFRILVKGLVSNDNLEKAMEIKEDMAVKGFVVDP 240

Query: 241 LIYHYLMGGCVRSLDPDGVFKLFEELKEKLGATVEDGVVYGNLMKGYFMKEMEEEAMKCY 300
           ++Y YLM GCV++ D DGV KL++ELKEKLG  V+DGVVYG LMKGYFMKEME+EAM+CY
Sbjct: 241 VVYSYLMMGCVKNSDADGVLKLYQELKEKLGGFVDDGVVYGQLMKGYFMKEMEKEAMECY 300

Query: 301 EETVGDNSVVKMSAIAYNSVLDALCRNGKFGEALTLFDRMTKEHRPPRHLAVNLGSFNVM 360
           EE VG+NS V+MSA+AYN VL+AL  NGKF EAL LFD + KEH PPRHLAVNLG+FNVM
Sbjct: 301 EEAVGENSKVRMSAMAYNYVLEALSENGKFDEALKLFDAVKKEHNPPRHLAVNLGTFNVM 360

Query: 361 VDGYCIEGRFKEAIEVFEKMGDYRCCPDTLSFNNLIEQLCNNGMLAEAEMLYGTMDDKGV 420
           V+GYC  G+F+EA+EVF +MGD++C PDTLSFNNL+ QLC+N +LAEAE LYG M++K V
Sbjct: 361 VNGYCAGGKFEEAMEVFRQMGDFKCSPDTLSFNNLMNQLCDNELLAEAEKLYGEMEEKNV 420

Query: 421 NPDEFTYGLLMDSCFKKNRADDAAAYFRKMVDSGLRPNIAVYNILVDELVKLGKIDDAKS 480
            PDE+TYGLLMD+CFK+ + D+ AAY++ MV+S LRPN+AVYN L D+L+K GK+DDAKS
Sbjct: 421 KPDEYTYGLLMDTCFKEGKIDEGAAYYKTMVESNLRPNLAVYNRLQDQLIKAGKLDDAKS 480

Query: 481 FFDLMVKKLKMDASSYQFIMKALSESGKMDEILNVVDTLLDDDGIEFSEELQEFVRGELR 540
           FFD+MV KLKMD  +Y+FIM+ALSE+G++DE+L +VD +LDDD +  SEELQEFV+ ELR
Sbjct: 481 FFDMMVSKLKMDDEAYKFIMRALSEAGRLDEMLKIVDEMLDDDTVRVSEELQEFVKEELR 540

Query: 541 KENREEDLAKLVEEKERLKAEAKAKEAEAAEAQKRSAKAAVSSLLSSKLFANKE 567
           K  RE DL KL+EEKERLKAEAKAKE   AE +K++    +++L+  K    K+
Sbjct: 541 KGGREGDLEKLMEEKERLKAEAKAKELADAEEKKKAQSINIAALIPPKAVEEKK 590

BLAST of Cucsa.032120 vs. Swiss-Prot
Match: PPR29_ARATH (Pentatricopeptide repeat-containing protein At1g10270 OS=Arabidopsis thaliana GN=GRP23 PE=1 SV=1)

HSP 1 Score: 297.4 bits (760), Expect = 3.7e-79
Identity = 182/519 (35.07%), Postives = 283/519 (54.53%), Query Frame = 1

Query: 17  ADAERRRRKRRLRIEPPLSSSSAARPLTQPPRSQTPQNPNAPKIPEHISALSGNRLNLHN 76
           A AERRRRKRRLRIEPPL +     P   PP+    ++PNAP++P+  SAL G RLNLHN
Sbjct: 96  AAAERRRRKRRLRIEPPLHALRRD-PSAPPPK----RDPNAPRLPDSTSALVGQRLNLHN 155

Query: 77  RILTLIRENDLEEAALFTRHSIYSNCRPTIFTVNAVLNAQLRQSKYADLLSLHR-FITQA 136
           R+ +LIR +DL+ A+   R S++SN RPT+FT NA++ A  R  +Y++ +SL + F  Q+
Sbjct: 156 RVQSLIRASDLDAASKLARQSVFSNTRPTVFTCNAIIAAMYRAKRYSESISLFQYFFKQS 215

Query: 137 GVVPNIITHNLIFQTYLDCRKPDTAMEHYKQLINDAPFNPSPTTYRILIKGLVDNNKLER 196
            +VPN++++N I   + D    D A+E Y+ ++ +APF PS  TYR L KGLV   ++  
Sbjct: 216 NIVPNVVSYNQIINAHCDEGNVDEALEVYRHILANAPFAPSSVTYRHLTKGLVQAGRIGD 275

Query: 197 AMELKDEMIEKGFAPDPLIYHYLMGGCVRSLDPDGVFKLFEELKEKLGATVEDGVVYGNL 256
           A  L  EM+ KG A D  +Y+ L+ G +   D D   + F+ELK K   TV DG+V    
Sbjct: 276 AASLLREMLSKGQAADSTVYNNLIRGYLDLGDFDKAVEFFDELKSK--CTVYDGIVNATF 335

Query: 257 MKGYFMKEMEEEAMKCYEETVGDNSVVKMSAIAYNSVLDALCRNGKFGEALTLFDRMTKE 316
           M+ +F K  ++EAM+ Y   +  +   +M     N +L+   + GK  EA  LF+ M   
Sbjct: 336 MEYWFEKGNDKEAMESYRSLL--DKKFRMHPPTGNVLLEVFLKFGKKDEAWALFNEMLDN 395

Query: 317 HRPPRHLAVNLGSFNVMVDGYCIEGRFKEAIEVFEKMGDYRCCP----DTLSFNNLIEQL 376
           H PP  L+VN  +  +MV+     G F EAI  F+K+G          D L + N++ + 
Sbjct: 396 HAPPNILSVNSDTVGIMVNECFKMGEFSEAINTFKKVGSKVTSKPFVMDYLGYCNIVTRF 455

Query: 377 CNNGMLAEAEMLYGTMDDKGVNPDEFTYGLLMDSCFKKNRADDAAAYFRKMVDSGLRPNI 436
           C  GML EAE  +     + +  D  ++  ++D+  K  R DDA     +MVD  LR   
Sbjct: 456 CEQGMLTEAERFFAEGVSRSLPADAPSHRAMIDAYLKAERIDDAVKMLDRMVDVNLRVVA 515

Query: 437 AVYNILVDELVKLGKIDDAKSFFDLM-VKKLKMDASSYQFIMKALSESGKMDEILNVVDT 496
                +  EL+K GK+ ++      M  ++ K D S Y  +++ L +   +D+  ++V  
Sbjct: 516 DFGARVFGELIKNGKLTESAEVLTKMGEREPKPDPSIYDVVVRGLCDGDALDQAKDIVGE 575

Query: 497 LLDDDGIEFSEELQEFVRGELRKENREEDLAKLVEEKER 530
           ++  + +  +  L+EF+     K  R E++ K++    R
Sbjct: 576 MIRHN-VGVTTVLREFIIEVFEKAGRREEIEKILNSVAR 604

BLAST of Cucsa.032120 vs. Swiss-Prot
Match: PPR28_ARATH (Pentatricopeptide repeat-containing protein At1g09900 OS=Arabidopsis thaliana GN=At1g09900 PE=2 SV=1)

HSP 1 Score: 171.8 bits (434), Expect = 2.3e-41
Identity = 108/412 (26.21%), Postives = 198/412 (48.06%), Query Frame = 1

Query: 75  HNRILTLIRENDLEEAALFTRHSIYSNCRPTIFTVNAVLNAQLRQSKYADLLSLHRFITQ 134
           +N +  ++R  +LEE   F  + +Y    P I     ++    R  K      +   +  
Sbjct: 106 NNHLRQMVRTGELEEGFKFLENMVYHGNVPDIIPCTTLIRGFCRLGKTRKAAKILEILEG 165

Query: 135 AGVVPNIITHNLIFQTYLDCRKPDTAMEHYKQLINDAPFNPSPTTYRILIKGLVDNNKLE 194
           +G VP++IT+N++   Y    + + A+     +++    +P   TY  +++ L D+ KL+
Sbjct: 166 SGAVPDVITYNVMISGYCKAGEINNALS----VLDRMSVSPDVVTYNTILRSLCDSGKLK 225

Query: 195 RAMELKDEMIEKGFAPDPLIYHYLMGGCVRSLDPDGVFKLFEELKEKLGATVEDGVVYGN 254
           +AME+ D M+++   PD + Y  L+    R        KL +E++++ G T  D V Y  
Sbjct: 226 QAMEVLDRMLQRDCYPDVITYTILIEATCRDSGVGHAMKLLDEMRDR-GCT-PDVVTYNV 285

Query: 255 LMKGYFMKEMEEEAMKCYEETVGDNSVVKMSAIAYNSVLDALCRNGKFGEALTLFDRMTK 314
           L+ G   +   +EA+K   +    +S  + + I +N +L ++C  G++ +A  L   M +
Sbjct: 286 LVNGICKEGRLDEAIKFLNDM--PSSGCQPNVITHNIILRSMCSTGRWMDAEKLLADMLR 345

Query: 315 EHRPPRHLAVNLGSFNVMVDGYCIEGRFKEAIEVFEKMGDYRCCPDTLSFNNLIEQLCNN 374
           +   P     ++ +FN++++  C +G    AI++ EKM  + C P++LS+N L+   C  
Sbjct: 346 KGFSP-----SVVTFNILINFLCRKGLLGRAIDILEKMPQHGCQPNSLSYNPLLHGFCKE 405

Query: 375 GMLAEAEMLYGTMDDKGVNPDEFTYGLLMDSCFKKNRADDAAAYFRKMVDSGLRPNIAVY 434
             +  A      M  +G  PD  TY  ++ +  K  + +DA     ++   G  P +  Y
Sbjct: 406 KKMDRAIEYLERMVSRGCYPDIVTYNTMLTALCKDGKVEDAVEILNQLSSKGCSPVLITY 465

Query: 435 NILVDELVKLGKIDDAKSFFDLM-VKKLKMDASSYQFIMKALSESGKMDEIL 486
           N ++D L K GK   A    D M  K LK D  +Y  ++  LS  GK+DE +
Sbjct: 466 NTVIDGLAKAGKTGKAIKLLDEMRAKDLKPDTITYSSLVGGLSREGKVDEAI 504


HSP 2 Score: 122.5 bits (306), Expect = 1.6e-26
Identity = 88/397 (22.17%), Postives = 185/397 (46.60%), Query Frame = 1

Query: 75  HNRIL-TLIRENDLEEAALFTRHSIYSNCRPTIFTVNAVLNAQLRQSKYADLLSLHRFIT 134
           +N IL +L     L++A       +  +C P + T   ++ A  R S     + L   + 
Sbjct: 207 YNTILRSLCDSGKLKQAMEVLDRMLQRDCYPDVITYTILIEATCRDSGVGHAMKLLDEMR 266

Query: 135 QAGVVPNIITHNLIFQTYLDCRKPDTAMEHYKQLINDAPFN---PSPTTYRILIKGLVDN 194
             G  P+++T+N++        + D A++     +ND P +   P+  T+ I+++ +   
Sbjct: 267 DRGCTPDVVTYNVLVNGICKEGRLDEAIK----FLNDMPSSGCQPNVITHNIILRSMCST 326

Query: 195 NKLERAMELKDEMIEKGFAPDPLIYHYLMGGCVRSLDPDGVFKLFEELKEKLGATVEDGV 254
            +   A +L  +M+ KGF+P  + ++ L+    R         + E++ +       + +
Sbjct: 327 GRWMDAEKLLADMLRKGFSPSVVTFNILINFLCRKGLLGRAIDILEKMPQH--GCQPNSL 386

Query: 255 VYGNLMKGYFMKEMEEEAMKCYEETVGDNSVVKMSAIAYNSVLDALCRNGKFGEALTLFD 314
            Y  L+ G+  ++  + A++  E  V       +  + YN++L ALC++GK  +A+ + +
Sbjct: 387 SYNPLLHGFCKEKKMDRAIEYLERMVSRGCYPDI--VTYNTMLTALCKDGKVEDAVEILN 446

Query: 315 RMTKEHRPPRHLAVNLGSFNVMVDGYCIEGRFKEAIEVFEKMGDYRCCPDTLSFNNLIEQ 374
           +++ +   P      L ++N ++DG    G+  +AI++ ++M      PDT+++++L+  
Sbjct: 447 QLSSKGCSPV-----LITYNTVIDGLAKAGKTGKAIKLLDEMRAKDLKPDTITYSSLVGG 506

Query: 375 LCNNGMLAEAEMLYGTMDDKGVNPDEFTYGLLMDSCFKKNRADDAAAYFRKMVDSGLRPN 434
           L   G + EA   +   +  G+ P+  T+  +M    K  + D A  +   M++ G +PN
Sbjct: 507 LSREGKVDEAIKFFHEFERMGIRPNAVTFNSIMLGLCKSRQTDRAIDFLVFMINRGCKPN 566

Query: 435 IAVYNILVDELVKLGKIDDAKSFFDLMVKKLKMDASS 468
              Y IL++ L   G   +A    + +  K  M  SS
Sbjct: 567 ETSYTILIEGLAYEGMAKEALELLNELCNKGLMKKSS 590


HSP 3 Score: 116.3 bits (290), Expect = 1.2e-24
Identity = 97/402 (24.13%), Postives = 178/402 (44.28%), Query Frame = 1

Query: 136 GVVPNIITHNLIFQTYLDCRKPDTAMEHYKQLINDAPFNPSPTTYRILIKGLVDNNKLER 195
           G VP+II    + + +    K   A +   +++  +   P   TY ++I G     ++  
Sbjct: 132 GNVPDIIPCTTLIRGFCRLGKTRKAAK-ILEILEGSGAVPDVITYNVMISGYCKAGEINN 191

Query: 196 AMELKDEMIEKGFAPDPLIYHYLMGGCVRSLDPDGVFKLFEELKEKL--GATVEDGVVYG 255
           A+ + D M     +PD + Y+ ++    RSL   G  K   E+ +++       D + Y 
Sbjct: 192 ALSVLDRM---SVSPDVVTYNTIL----RSLCDSGKLKQAMEVLDRMLQRDCYPDVITYT 251

Query: 256 NLMKGYFMKEMEEEAMKCYEETVGDNSVVKMSAIAYNSVLDALCRNGKFGEALTLFDRMT 315
            L++          AMK  +E + D        + YN +++ +C+ G+  EA+   + M 
Sbjct: 252 ILIEATCRDSGVGHAMKLLDE-MRDRGCTP-DVVTYNVLVNGICKEGRLDEAIKFLNDMP 311

Query: 316 KEHRPPRHLAVNLGSFNVMVDGYCIEGRFKEAIEVFEKMGDYRCCPDTLSFNNLIEQLCN 375
                P     N+ + N+++   C  GR+ +A ++   M      P  ++FN LI  LC 
Sbjct: 312 SSGCQP-----NVITHNIILRSMCSTGRWMDAEKLLADMLRKGFSPSVVTFNILINFLCR 371

Query: 376 NGMLAEAEMLYGTMDDKGVNPDEFTYGLLMDSCFKKNRADDAAAYFRKMVDSGLRPNIAV 435
            G+L  A  +   M   G  P+  +Y  L+    K+ + D A  Y  +MV  G  P+I  
Sbjct: 372 KGLLGRAIDILEKMPQHGCQPNSLSYNPLLHGFCKEKKMDRAIEYLERMVSRGCYPDIVT 431

Query: 436 YNILVDELVKLGKIDDAKSFFD-LMVKKLKMDASSYQFIMKALSESGKMDEILNVVDTL- 495
           YN ++  L K GK++DA    + L  K       +Y  ++  L+++GK  + + ++D + 
Sbjct: 432 YNTMLTALCKDGKVEDAVEILNQLSSKGCSPVLITYNTVIDGLAKAGKTGKAIKLLDEMR 491

Query: 496 ---LDDDGIEFSEELQEFVRGELRKENREEDLAKLVEEKERL 531
              L  D I +S      + G L +E + ++  K   E ER+
Sbjct: 492 AKDLKPDTITYSS-----LVGGLSREGKVDEAIKFFHEFERM 513

BLAST of Cucsa.032120 vs. Swiss-Prot
Match: PPR36_ARATH (Pentatricopeptide repeat-containing protein At1g12300, mitochondrial OS=Arabidopsis thaliana GN=At1g12300 PE=2 SV=1)

HSP 1 Score: 165.2 bits (417), Expect = 2.2e-39
Identity = 109/419 (26.01%), Postives = 199/419 (47.49%), Query Frame = 1

Query: 65  SALSGNRLNLHNRILTLIRENDLEEAALFTRHSIYSNCRPTIFTVNAVLNAQLRQSKYAD 124
           SA S   L+   R+ + + +   ++A    R  I+S   PT+   + + +A  +  +Y  
Sbjct: 47  SAFSDRNLSYRERLRSGLVDIKADDAIDLFRDMIHSRPLPTVIDFSRLFSAIAKTKQYDL 106

Query: 125 LLSLHRFITQAGVVPNIITHNLIFQTYLDCRKPDTAMEHYKQLINDAPFNPSPTTYRILI 184
           +L+L + +   G+  N+ T +++   +  CRK   A     ++I    + P+  T+  LI
Sbjct: 107 VLALCKQMELKGIAHNLYTLSIMINCFCRCRKLCLAFSAMGKIIK-LGYEPNTITFSTLI 166

Query: 185 KGLVDNNKLERAMELKDEMIEKGFAPDPLIYHYLMGGCVRSLDPDGVFKLFEELKEKLGA 244
            GL    ++  A+EL D M+E G  PD +  + L+ G   S        L +++ E    
Sbjct: 167 NGLCLEGRVSEALELVDRMVEMGHKPDLITINTLVNGLCLSGKEAEAMLLIDKMVEY--G 226

Query: 245 TVEDGVVYGNLMKGYFMKEMEEEAMKCYEETVGDNSVVKMSAIAYNSVLDALCRNGKFGE 304
              + V YG ++           AM+   +    N  +K+ A+ Y+ ++D LC++G    
Sbjct: 227 CQPNAVTYGPVLNVMCKSGQTALAMELLRKMEERN--IKLDAVKYSIIIDGLCKHGSLDN 286

Query: 305 ALTLFDRMTKEHRPPRHLAVNLGSFNVMVDGYCIEGRFKEAIEVFEKMGDYRCCPDTLSF 364
           A  LF+ M       + +  N+ ++N+++ G+C  GR+ +  ++   M   +  P+ ++F
Sbjct: 287 AFNLFNEMEM-----KGITTNIITYNILIGGFCNAGRWDDGAKLLRDMIKRKINPNVVTF 346

Query: 365 NNLIEQLCNNGMLAEAEMLYGTMDDKGVNPDEFTYGLLMDSCFKKNRADDAAAYFRKMVD 424
           + LI+     G L EAE L+  M  +G+ PD  TY  L+D   K+N  D A      MV 
Sbjct: 347 SVLIDSFVKEGKLREAEELHKEMIHRGIAPDTITYTSLIDGFCKENHLDKANQMVDLMVS 406

Query: 425 SGLRPNIAVYNILVDELVKLGKIDDAKSFFDLM-VKKLKMDASSYQFIMKALSESGKMD 483
            G  PNI  +NIL++   K  +IDD    F  M ++ +  D  +Y  +++   E GK++
Sbjct: 407 KGCDPNIRTFNILINGYCKANRIDDGLELFRKMSLRGVVADTVTYNTLIQGFCELGKLN 455


HSP 2 Score: 154.8 bits (390), Expect = 2.9e-36
Identity = 113/462 (24.46%), Postives = 205/462 (44.37%), Query Frame = 1

Query: 70  NRLNLHNRILTLIRENDLEEAALFTRHSIYSNCRPTIFTVNAVLNAQLRQSKYADLLSLH 129
           N +     I  L  E  + EA       +    +P + T+N ++N      K A+ + L 
Sbjct: 157 NTITFSTLINGLCLEGRVSEALELVDRMVEMGHKPDLITINTLVNGLCLSGKEAEAMLLI 216

Query: 130 RFITQAGVVPNIITHNLIFQTYLDCRKPDTAMEHYKQLINDAPFNPSPTTYRILIKGLVD 189
             + + G  PN +T+  +        +   AME  +++  +         Y I+I GL  
Sbjct: 217 DKMVEYGCQPNAVTYGPVLNVMCKSGQTALAMELLRKM-EERNIKLDAVKYSIIIDGLCK 276

Query: 190 NNKLERAMELKDEMIEKGFAPDPLIYHYLMGG-CVRSLDPDGVFKLFEELKEKLGATVED 249
           +  L+ A  L +EM  KG   + + Y+ L+GG C      DG   L + +K K+   V  
Sbjct: 277 HGSLDNAFNLFNEMEMKGITTNIITYNILIGGFCNAGRWDDGAKLLRDMIKRKINPNV-- 336

Query: 250 GVVYGNLMKGYFMKEMEEEAMKCYEETVGDNSVVKMSAIAYNSVLDALCRNGKFGEALTL 309
            V +  L+  +  +    EA + ++E +  +  +    I Y S++D  C+     +A  +
Sbjct: 337 -VTFSVLIDSFVKEGKLREAEELHKEMI--HRGIAPDTITYTSLIDGFCKENHLDKANQM 396

Query: 310 FDRMTKEHRPPRHLAVNLGSFNVMVDGYCIEGRFKEAIEVFEKMGDYRCCPDTLSFNNLI 369
            D M  +   P     N+ +FN++++GYC   R  + +E+F KM       DT+++N LI
Sbjct: 397 VDLMVSKGCDP-----NIRTFNILINGYCKANRIDDGLELFRKMSLRGVVADTVTYNTLI 456

Query: 370 EQLCNNGMLAEAEMLYGTMDDKGVNPDEFTYGLLMDSCFKKNRADDAAAYFRKMVDSGLR 429
           +  C  G L  A+ L+  M  + V P+  TY +L+D       ++ A   F K+  S + 
Sbjct: 457 QGFCELGKLNVAKELFQEMVSRKVPPNIVTYKILLDGLCDNGESEKALEIFEKIEKSKME 516

Query: 430 PNIAVYNILVDELVKLGKIDDAKSFF-DLMVKKLKMDASSYQFIMKALSESGKMDEILNV 489
            +I +YNI++  +    K+DDA   F  L +K +K    +Y  ++  L + G + E   +
Sbjct: 517 LDIGIYNIIIHGMCNASKVDDAWDLFCSLPLKGVKPGVKTYNIMIGGLCKKGPLSEAELL 576

Query: 490 VDTLLDDDGIEFSEELQEFVRGELRKENREEDLAKLVEEKER 530
              + +D            +R  L   +  + + KL+EE +R
Sbjct: 577 FRKMEEDGHAPDGWTYNILIRAHLGDGDATKSV-KLIEELKR 606


HSP 3 Score: 110.5 bits (275), Expect = 6.4e-23
Identity = 89/362 (24.59%), Postives = 174/362 (48.07%), Query Frame = 1

Query: 173 FNPSPTTYRILIK-GLVDNNKLERAMELKDEMIEKGFAPDPLIYHYLMGGCVRSLDPDGV 232
           F+    +YR  ++ GLVD  K + A++L  +MI     P  + +  L     ++   D V
Sbjct: 49  FSDRNLSYRERLRSGLVDI-KADDAIDLFRDMIHSRPLPTVIDFSRLFSAIAKTKQYDLV 108

Query: 233 FKLFEELKEKLGATVEDGVVYGNLMKGYFMKEMEEEAMKCYEETVGDNSVVKM------- 292
             L ++++ K       G+ + NL   Y +  M     +C +  +  +++ K+       
Sbjct: 109 LALCKQMELK-------GIAH-NL---YTLSIMINCFCRCRKLCLAFSAMGKIIKLGYEP 168

Query: 293 SAIAYNSVLDALCRNGKFGEALTLFDRMTKEHRPPRHLAVNLGSFNVMVDGYCIEGRFKE 352
           + I ++++++ LC  G+  EAL L DRM +    P     +L + N +V+G C+ G+  E
Sbjct: 169 NTITFSTLINGLCLEGRVSEALELVDRMVEMGHKP-----DLITINTLVNGLCLSGKEAE 228

Query: 353 AIEVFEKMGDYRCCPDTLSFNNLIEQLCNNGMLAEAEMLYGTMDDKGVNPDEFTYGLLMD 412
           A+ + +KM +Y C P+ +++  ++  +C +G  A A  L   M+++ +  D   Y +++D
Sbjct: 229 AMLLIDKMVEYGCQPNAVTYGPVLNVMCKSGQTALAMELLRKMEERNIKLDAVKYSIIID 288

Query: 413 SCFKKNRADDAAAYFRKMVDSGLRPNIAVYNILVDELVKLGKIDD-AKSFFDLMVKKLKM 472
              K    D+A   F +M   G+  NI  YNIL+      G+ DD AK   D++ +K+  
Sbjct: 289 GLCKHGSLDNAFNLFNEMEMKGITTNIITYNILIGGFCNAGRWDDGAKLLRDMIKRKINP 348

Query: 473 DASSYQFIMKALSESGKMDEI----LNVVDTLLDDDGIEFSEELQEFVR-GELRKENREE 521
           +  ++  ++ +  + GK+ E       ++   +  D I ++  +  F +   L K N+  
Sbjct: 349 NVVTFSVLIDSFVKEGKLREAEELHKEMIHRGIAPDTITYTSLIDGFCKENHLDKANQMV 393

BLAST of Cucsa.032120 vs. Swiss-Prot
Match: PPR97_ARATH (Pentatricopeptide repeat-containing protein At1g63070, mitochondrial OS=Arabidopsis thaliana GN=At1g63070 PE=2 SV=1)

HSP 1 Score: 159.5 bits (402), Expect = 1.2e-37
Identity = 107/425 (25.18%), Postives = 210/425 (49.41%), Query Frame = 1

Query: 106 IFTVNAVLNAQLRQSKYADLLSLHRFITQAGVVPNIITHNLIFQTYLDCRKPDTAMEHYK 165
           ++T +  +N   R+S+ +  L++   + + G  P+I+T N +   +    +   A+    
Sbjct: 110 LYTYSIFINYFCRRSQLSLALAILGKMMKLGYGPSIVTLNSLLNGFCHGNRISEAVALVD 169

Query: 166 QLINDAPFNPSPTTYRILIKGLVDNNKLERAMELKDEMIEKGFAPDPLIYHYLMGGCVRS 225
           Q++ +  + P   T+  L+ GL  +NK   A+ L + M+ KG  PD + Y  ++ G  + 
Sbjct: 170 QMV-EMGYQPDTVTFTTLVHGLFQHNKASEAVALVERMVVKGCQPDLVTYGAVINGLCKR 229

Query: 226 LDPDGVFKLFEELKEKLGATVEDGVVYGNLMKGYFMKEMEEEAMKCYEETVGDNSVVKMS 285
            +PD    L  ++++  G    D V+Y  ++ G    +  ++A   + +   +   +K  
Sbjct: 230 GEPDLALNLLNKMEK--GKIEADVVIYNTIIDGLCKYKHMDDAFDLFNKM--ETKGIKPD 289

Query: 286 AIAYNSVLDALCRNGKFGEALTLFDRMTKEHRPPRHLAVNLGSFNVMVDGYCIEGRFKEA 345
              YN ++  LC  G++ +A  L   M +++  P     +L  FN ++D +  EG+  EA
Sbjct: 290 VFTYNPLISCLCNYGRWSDASRLLSDMLEKNINP-----DLVFFNALIDAFVKEGKLVEA 349

Query: 346 IEVFEKMGDYR-CCPDTLSFNNLIEQLCNNGMLAEAEMLYGTMDDKGVNPDEFTYGLLMD 405
            +++++M   + C PD +++N LI+  C    + E   ++  M  +G+  +  TY  L+ 
Sbjct: 350 EKLYDEMVKSKHCFPDVVAYNTLIKGFCKYKRVEEGMEVFREMSQRGLVGNTVTYTTLIH 409

Query: 406 SCFKKNRADDAAAYFRKMVDSGLRPNIAVYNILVDELVKLGKIDDAKSFFDLMVKK-LKM 465
             F+    D+A   F++MV  G+ P+I  YNIL+D L   G ++ A   F+ M K+ +K+
Sbjct: 410 GFFQARDCDNAQMVFKQMVSDGVHPDIMTYNILLDGLCNNGNVETALVVFEYMQKRDMKL 469

Query: 466 DASSYQFIMKALSESGKMDEILNVVDTLLDDDGIEFSEELQEFVRGELRKENREEDLAKL 525
           D  +Y  +++AL ++GK+++  ++  +L               + G  RK  +EE  A  
Sbjct: 470 DIVTYTTMIEALCKAGKVEDGWDLFCSLSLKGVKPNVVTYTTMMSGFCRKGLKEEADALF 524

Query: 526 VEEKE 529
           VE KE
Sbjct: 530 VEMKE 524


HSP 2 Score: 130.2 bits (326), Expect = 7.8e-29
Identity = 102/428 (23.83%), Postives = 190/428 (44.39%), Query Frame = 1

Query: 71  RLNLHNRILTLIRENDLEEAALFTRHSIYSNCRPTIFTVNAVLNAQLRQSKYADLLSLHR 130
           R NL  ++L  ++   L++A       + S   P+I   + +L+A  + +K+  ++SL  
Sbjct: 43  RENLSRKVLQDLK---LDDAIGLFGDMVKSRPFPSIVEFSKLLSAIAKMNKFDLVISLGE 102

Query: 131 FITQAGVVPNIITHNLIFQTYLDCRKPDTAMEHYKQLINDAPFNPSPTTYRILIKGLVDN 190
            +   G+  N+ T+++    +    +   A+    +++    + PS  T   L+ G    
Sbjct: 103 QMQNLGISHNLYTYSIFINYFCRRSQLSLALAILGKMMK-LGYGPSIVTLNSLLNGFCHG 162

Query: 191 NKLERAMELKDEMIEKGFAPDPLIYHYLMGGCVRSLDPDGVFKLFEELKEKLGATVEDGV 250
           N++  A+ L D+M+E G+ PD + +  L+ G  +         L E +  K      D V
Sbjct: 163 NRISEAVALVDQMVEMGYQPDTVTFTTLVHGLFQHNKASEAVALVERMVVK--GCQPDLV 222

Query: 251 VYGNLMKGYFMKEMEEEAMKCYEETVGDNSVVKMSAIAYNSVLDALCRNGKFGEALTLFD 310
            YG ++ G   +   + A+    +   +   ++   + YN+++D LC+     +A  LF+
Sbjct: 223 TYGAVINGLCKRGEPDLALNLLNKM--EKGKIEADVVIYNTIIDGLCKYKHMDDAFDLFN 282

Query: 311 RM-TKEHRPPRHLAVNLGSFNVMVDGYCIEGRFKEAIEVFEKMGDYRCCPDTLSFNNLIE 370
           +M TK  +P      ++ ++N ++   C  GR+ +A  +   M +    PD + FN LI+
Sbjct: 283 KMETKGIKP------DVFTYNPLISCLCNYGRWSDASRLLSDMLEKNINPDLVFFNALID 342

Query: 371 QLCNNGMLAEAEMLYGTM-DDKGVNPDEFTYGLLMDSCFKKNRADDAAAYFRKMVDSGLR 430
                G L EAE LY  M   K   PD   Y  L+    K  R ++    FR+M   GL 
Sbjct: 343 AFVKEGKLVEAEKLYDEMVKSKHCFPDVVAYNTLIKGFCKYKRVEEGMEVFREMSQRGLV 402

Query: 431 PNIAVYNILVDELVKLGKIDDAKSFFDLMVKK-LKMDASSYQFIMKALSESGKMDEILNV 490
            N   Y  L+    +    D+A+  F  MV   +  D  +Y  ++  L  +G ++  L V
Sbjct: 403 GNTVTYTTLIHGFFQARDCDNAQMVFKQMVSDGVHPDIMTYNILLDGLCNNGNVETALVV 456

Query: 491 VDTLLDDD 496
            + +   D
Sbjct: 463 FEYMQKRD 456


HSP 3 Score: 124.0 bits (310), Expect = 5.6e-27
Identity = 97/408 (23.77%), Postives = 187/408 (45.83%), Query Frame = 1

Query: 81  LIRENDLEEAALFTRHSIYSNCRPTIFTVNAVLNAQLRQSKYADLLSLHRFITQAGVVPN 140
           L + N   EA       +   C+P + T  AV+N   ++ +    L+L   + +  +  +
Sbjct: 190 LFQHNKASEAVALVERMVVKGCQPDLVTYGAVINGLCKRGEPDLALNLLNKMEKGKIEAD 249

Query: 141 IITHNLIFQTYLDCRKPDTAMEHYKQLINDAPFNPSPTTYRILIKGLVDNNKLERAMELK 200
           ++ +N I       +  D A + + ++       P   TY  LI  L +  +   A  L 
Sbjct: 250 VVIYNTIIDGLCKYKHMDDAFDLFNKMETKG-IKPDVFTYNPLISCLCNYGRWSDASRLL 309

Query: 201 DEMIEKGFAPDPLIYHYLMGGCVRSLDPDGVFKLFEELKEKLGATVEDGVVYGNLMKGYF 260
            +M+EK   PD + ++ L+   V+        KL++E+  K      D V Y  L+KG+ 
Sbjct: 310 SDMLEKNINPDLVFFNALIDAFVKEGKLVEAEKLYDEMV-KSKHCFPDVVAYNTLIKGFC 369

Query: 261 MKEMEEEAMKCYEETVGDNSVVKMSAIAYNSVLDALCRNGKFGEALTLFDRMTKEHRPPR 320
             +  EE M+ + E +    +V  + + Y +++    +      A  +F +M  +   P 
Sbjct: 370 KYKRVEEGMEVFRE-MSQRGLVG-NTVTYTTLIHGFFQARDCDNAQMVFKQMVSDGVHP- 429

Query: 321 HLAVNLGSFNVMVDGYCIEGRFKEAIEVFEKMGDYRCCPDTLSFNNLIEQLCNNGMLAEA 380
               ++ ++N+++DG C  G  + A+ VFE M       D +++  +IE LC  G + + 
Sbjct: 430 ----DIMTYNILLDGLCNNGNVETALVVFEYMQKRDMKLDIVTYTTMIEALCKAGKVEDG 489

Query: 381 EMLYGTMDDKGVNPDEFTYGLLMDSCFKKNRADDAAAYFRKMVDSGLRPNIAVYNILVDE 440
             L+ ++  KGV P+  TY  +M    +K   ++A A F +M + G  PN   YN L+  
Sbjct: 490 WDLFCSLSLKGVKPNVVTYTTMMSGFCRKGLKEEADALFVEMKEDGPLPNSGTYNTLIRA 549

Query: 441 LVKLGKIDDAKSFFDLMVKKLKM-----DASSYQFIMKALSESGKMDE 484
            ++ G  D+A S    ++K+++      DAS++  +   L + G++D+
Sbjct: 550 RLRDG--DEAAS--AELIKEMRSCGFAGDASTFGLVTNMLHD-GRLDK 583

BLAST of Cucsa.032120 vs. TrEMBL
Match: A0A0A0L7B2_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G239860 PE=4 SV=1)

HSP 1 Score: 1120.5 bits (2897), Expect = 0.0e+00
Identity = 573/611 (93.78%), Postives = 575/611 (94.11%), Query Frame = 1

Query: 1   MALSKPAFFTHLKTLT-----------------------------ADAERRRRKRRLRIE 60
           MALSKPAFFTHLKTLT                             ADAERRRRKRRLRIE
Sbjct: 1   MALSKPAFFTHLKTLTGSHHLLQRQALAPFPIVTLRFLSFASAEEADAERRRRKRRLRIE 60

Query: 61  PPLSSSSAARPLTQPPRSQTPQNPNAPKIPEHISALSGNRLNLHNRILTLIRENDLEEAA 120
           PPLSSSSAARPLTQPPRSQTPQNPNAPKIPEHISALSGNRLNLHNRILTLIRENDLEEAA
Sbjct: 61  PPLSSSSAARPLTQPPRSQTPQNPNAPKIPEHISALSGNRLNLHNRILTLIRENDLEEAA 120

Query: 121 LFTRHSIYSNCRPTIFTVNAVLNAQLRQSKYADLLSLHRFITQAGVVPNIITHNLIFQTY 180
           LFTRHSIYSNCRPTIFTVNAVLNAQLRQSKYADLLSLHRFITQAGVVPNIITHNLIFQTY
Sbjct: 121 LFTRHSIYSNCRPTIFTVNAVLNAQLRQSKYADLLSLHRFITQAGVVPNIITHNLIFQTY 180

Query: 181 LDCRKPDTAMEHYKQLINDAPFNPSPTTYRILIKGLVDNNKLERAMELKDEMIEKGFAPD 240
           LDCRKPDTAMEHYKQLINDAPFNPSPTTYRILIKGLVDNNKLERAMELKDEMIEKGFAPD
Sbjct: 181 LDCRKPDTAMEHYKQLINDAPFNPSPTTYRILIKGLVDNNKLERAMELKDEMIEKGFAPD 240

Query: 241 PLIYHYLMGGCVRSLDPDGVFKLFEELKEKLGATVEDGVVYGNLMKGYFMKEMEEEAMKC 300
           PLIYHYLMGGCVRSLDPDGVFKLFEELKEKLGATVEDGVVYGNLMKGYFMKEMEEEAMKC
Sbjct: 241 PLIYHYLMGGCVRSLDPDGVFKLFEELKEKLGATVEDGVVYGNLMKGYFMKEMEEEAMKC 300

Query: 301 YEETVGDNSVVKMSAIAYNSVLDALCRNGKFGEALTLFDRMTKEHRPPRHLAVNLGSFNV 360
           YEETVGDNSVVKMSAIAYNSVLDALCRNGKFGEALTLFDRMTKEHRPPRHLAVNLGSFNV
Sbjct: 301 YEETVGDNSVVKMSAIAYNSVLDALCRNGKFGEALTLFDRMTKEHRPPRHLAVNLGSFNV 360

Query: 361 MVDGYCIEGRFKEAIEVFEKMGDYRCCPDTLSFNNLIEQLCNNGMLAEAEMLYGTMDDKG 420
           MVDGYCIEGRFKEAIEVFEKMGDYRCCPDTLSFNNLIEQLCNNGMLAEAEMLYGTMDDKG
Sbjct: 361 MVDGYCIEGRFKEAIEVFEKMGDYRCCPDTLSFNNLIEQLCNNGMLAEAEMLYGTMDDKG 420

Query: 421 VNPDEFTYGLLMDSCFKKNRADDAAAYFRKMVDSGLRPNIAVYNILVDELVKLGKIDDAK 480
           VNPDEFTYGLLMDSCFKKNRADDAAAYFRKMVDSGLRPNIAVYNILVDELVKLGKIDDAK
Sbjct: 421 VNPDEFTYGLLMDSCFKKNRADDAAAYFRKMVDSGLRPNIAVYNILVDELVKLGKIDDAK 480

Query: 481 SFFDLMVKKLKMDASSYQFIMKALSESGKMDEILNVVDTLLDDDGIEFSEELQEFVRGEL 540
           SFFDLMVKKLKMDASSYQFIMKALSESGKMDEILNVVDTLLDDDGIEFSEELQEFVRGEL
Sbjct: 481 SFFDLMVKKLKMDASSYQFIMKALSESGKMDEILNVVDTLLDDDGIEFSEELQEFVRGEL 540

Query: 541 RKENREEDLAKLVEEKERLKAEAKAKEAEAAEAQKRSAKAAVSSLLSSKLFANKEGEKES 583
           RKENREEDLAKLVEEKERLKAEAKAKEAEAAEAQKRSAKAAVSSLLSSKLFANKEGEKES
Sbjct: 541 RKENREEDLAKLVEEKERLKAEAKAKEAEAAEAQKRSAKAAVSSLLSSKLFANKEGEKES 600

BLAST of Cucsa.032120 vs. TrEMBL
Match: E5GB98_CUCME (Pentatricopeptide repeat-containing protein OS=Cucumis melo subsp. melo PE=4 SV=1)

HSP 1 Score: 1063.9 bits (2750), Expect = 7.2e-308
Identity = 542/601 (90.18%), Postives = 557/601 (92.68%), Query Frame = 1

Query: 1   MALSKPAFFTHLKTLT-----------------------------ADAERRRRKRRLRIE 60
           MALSKPAFFTHLKTLT                             ADAERRRRKRRLRIE
Sbjct: 1   MALSKPAFFTHLKTLTGSHHLLQRQAPAPLPIVTFRFLSFASAEEADAERRRRKRRLRIE 60

Query: 61  PPLSSSSAARPLTQPPRSQTPQNPNAPKIPEHISALSGNRLNLHNRILTLIRENDLEEAA 120
           PPLSSSSAARP +QP RSQTPQNPN PK+PEHISALSGNRLNLHNRILTLIRENDLEEAA
Sbjct: 61  PPLSSSSAARPQSQPSRSQTPQNPNTPKVPEHISALSGNRLNLHNRILTLIRENDLEEAA 120

Query: 121 LFTRHSIYSNCRPTIFTVNAVLNAQLRQSKYADLLSLHRFITQAGVVPNIITHNLIFQTY 180
           LFTRHSIYSNCRPTIFTVNAVLNAQLRQSKYADLLSLHRFITQAGVVPNIITHNLIFQTY
Sbjct: 121 LFTRHSIYSNCRPTIFTVNAVLNAQLRQSKYADLLSLHRFITQAGVVPNIITHNLIFQTY 180

Query: 181 LDCRKPDTAMEHYKQLINDAPFNPSPTTYRILIKGLVDNNKLERAMELKDEMIEKGFAPD 240
           LDCRKPDTAMEHYKQLINDAPFNPSPTTYRILIKGLVDN KLERAMELK+EMI KGFAPD
Sbjct: 181 LDCRKPDTAMEHYKQLINDAPFNPSPTTYRILIKGLVDNKKLERAMELKEEMIVKGFAPD 240

Query: 241 PLIYHYLMGGCVRSLDPDGVFKLFEELKEKLGATVEDGVVYGNLMKGYFMKEMEEEAMKC 300
           PLIYHYLM GCVRS DPDGVFKLFEELKEKLG TVEDGVVYGNLMKGYFMKEMEEEAMKC
Sbjct: 241 PLIYHYLMAGCVRSSDPDGVFKLFEELKEKLGGTVEDGVVYGNLMKGYFMKEMEEEAMKC 300

Query: 301 YEETVGDNSVVKMSAIAYNSVLDALCRNGKFGEALTLFDRMTKEHRPPRHLAVNLGSFNV 360
           YEETVGDN VVKMSAIAYNSVLDALC++GKF EALTLFDRMTKEHRPPRHLAVNLG+FNV
Sbjct: 301 YEETVGDNPVVKMSAIAYNSVLDALCKHGKFSEALTLFDRMTKEHRPPRHLAVNLGTFNV 360

Query: 361 MVDGYCIEGRFKEAIEVFEKMGDYRCCPDTLSFNNLIEQLCNNGMLAEAEMLYGTMDDKG 420
           MVDGYCI+GRFKEAI VFE+MGDYRC PDTLSFNNLIEQLCNNGMLAEAEMLYGTM +KG
Sbjct: 361 MVDGYCIKGRFKEAIGVFEEMGDYRCSPDTLSFNNLIEQLCNNGMLAEAEMLYGTMGEKG 420

Query: 421 VNPDEFTYGLLMDSCFKKNRADDAAAYFRKMVDSGLRPNIAVYNILVDELVKLGKIDDAK 480
           VNPDEFTYGLLM SCF+KNRADDAAAYFRKMVDSGLRPNIAVYNILV ELVKLGK+D+AK
Sbjct: 421 VNPDEFTYGLLMHSCFQKNRADDAAAYFRKMVDSGLRPNIAVYNILVGELVKLGKVDEAK 480

Query: 481 SFFDLMVKKLKMDASSYQFIMKALSESGKMDEILNVVDTLLDDDGIEFSEELQEFVRGEL 540
           SFFDLMVKKLKMDAS+YQFIMKALSESGKMDE+LNVVDTLLDDDGIEFSEELQEFVRGEL
Sbjct: 481 SFFDLMVKKLKMDASNYQFIMKALSESGKMDEVLNVVDTLLDDDGIEFSEELQEFVRGEL 540

Query: 541 RKENREEDLAKLVEEKERLKAEAKAKEAEAAEAQKRSAKAAVSSLLSSKLFANKEGEKES 573
           RKE+REEDLAKLVEEKERLKAEAKAKEAEAAEAQKRSAKAAVSSLLSSKLFANKEGEKES
Sbjct: 541 RKEDREEDLAKLVEEKERLKAEAKAKEAEAAEAQKRSAKAAVSSLLSSKLFANKEGEKES 600

BLAST of Cucsa.032120 vs. TrEMBL
Match: M5X3R7_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa002582mg PE=4 SV=1)

HSP 1 Score: 908.7 bits (2347), Expect = 3.9e-261
Identity = 457/599 (76.29%), Postives = 510/599 (85.14%), Query Frame = 1

Query: 1   MALSKPAFFTHLKTLT---------------------------ADAERRRRKRRLRIEPP 60
           MALSKP F THL+TL                            A AERRRRKRRLRIEPP
Sbjct: 1   MALSKPTFLTHLRTLAKPPNCHHPTTPPSFISLRFLSFATPEEAAAERRRRKRRLRIEPP 60

Query: 61  LSSSSAARPLTQPPRSQTPQ-NPNAPKIPEHISALSGNRLNLHNRILTLIRENDLEEAAL 120
           LSS    +   Q  +S  PQ NPNAPK+PE +SALSGNRLNLHNRILTL+R+NDLEEAAL
Sbjct: 61  LSSLHRNQQQQQQQQSPKPQQNPNAPKLPEPVSALSGNRLNLHNRILTLVRQNDLEEAAL 120

Query: 121 FTRHSIYSNCRPTIFTVNAVLNAQLRQSKYADLLSLHRFITQAGVVPNIITHNLIFQTYL 180
           +TRHSIYSNCRPTIFTVN+VL AQLRQSKY+DLLSLHRFITQAGV PNIITHNLIFQTYL
Sbjct: 121 YTRHSIYSNCRPTIFTVNSVLTAQLRQSKYSDLLSLHRFITQAGVAPNIITHNLIFQTYL 180

Query: 181 DCRKPDTAMEHYKQLINDAPFNPSPTTYRILIKGLVDNNKLERAMELKDEMIEKGFAPDP 240
           DCRKPDTAME+YKQLINDAPFNPSPTTYRILIKGLVDNNKL+RAMELK+E+  KGFAPDP
Sbjct: 181 DCRKPDTAMENYKQLINDAPFNPSPTTYRILIKGLVDNNKLDRAMELKEEIDAKGFAPDP 240

Query: 241 LIYHYLMGGCVRSLDPDGVFKLFEELKEKLGATVEDGVVYGNLMKGYFMKEMEEEAMKCY 300
           ++YHYLM GCV++ D DGVF+L+EELKEKLG  VEDG+VYGNLMKGYFM+ ME+EAM+CY
Sbjct: 241 VVYHYLMVGCVKNSDSDGVFRLYEELKEKLGGVVEDGIVYGNLMKGYFMRGMEKEAMECY 300

Query: 301 EETVGDNSVVKMSAIAYNSVLDALCRNGKFGEALTLFDRMTKEHRPPRHLAVNLGSFNVM 360
           EE+ G++S VK SA+AYNSVLDAL +NGKF EAL LFDRM  EH PPR LAVNLGSFNVM
Sbjct: 301 EESFGESSKVKTSAVAYNSVLDALSKNGKFDEALRLFDRMVAEHNPPRRLAVNLGSFNVM 360

Query: 361 VDGYCIEGRFKEAIEVFEKMGDYRCCPDTLSFNNLIEQLCNNGMLAEAEMLYGTMDDKGV 420
            DGYC++GRFKEAIEVF KMGDYRC PDTLSFNNLIEQLC NGML+EAE LYG M DKGV
Sbjct: 361 ADGYCVQGRFKEAIEVFRKMGDYRCSPDTLSFNNLIEQLCKNGMLSEAEELYGEMSDKGV 420

Query: 421 NPDEFTYGLLMDSCFKKNRADDAAAYFRKMVDSGLRPNIAVYNILVDELVKLGKIDDAKS 480
            PDEFTY LLMD+CF++NRADDAA YFRKMVD+ LRPN+AVYN LVD L+K+GK+D+AKS
Sbjct: 421 YPDEFTYVLLMDTCFEENRADDAAEYFRKMVDAKLRPNLAVYNRLVDGLIKVGKVDEAKS 480

Query: 481 FFDLMVKKLKMDASSYQFIMKALSESGKMDEILNVVDTLLDDDGIEFSEELQEFVRGELR 540
           FFDLMVKKLKMD  SYQFIMK LSE+GK+DE+LNVVDT+LDDDG+EF+EELQEFV+GELR
Sbjct: 481 FFDLMVKKLKMDIPSYQFIMKTLSEAGKLDEVLNVVDTMLDDDGVEFNEELQEFVKGELR 540

Query: 541 KENREEDLAKLVEEKERLKAEAKAKEAEAAEAQKRSAKAAVSSLLSSKLFANKEGEKES 572
           KE RE+++ KL+EEKER KAEAKAKEAEAAEA KRSA+AAVSSLL SKLF NKE E  S
Sbjct: 541 KEGREDEVGKLMEEKERQKAEAKAKEAEAAEAAKRSARAAVSSLLPSKLFGNKESETGS 599

BLAST of Cucsa.032120 vs. TrEMBL
Match: W9RP26_9ROSA (Uncharacterized protein OS=Morus notabilis GN=L484_006813 PE=4 SV=1)

HSP 1 Score: 881.7 bits (2277), Expect = 5.1e-253
Identity = 456/650 (70.15%), Postives = 514/650 (79.08%), Query Frame = 1

Query: 1   MALSKP-AFFTHLKTLT------------------------------ADAERRRRKRRLR 60
           MALSKP AF THLKTL                               A AERRRRKRRLR
Sbjct: 1   MALSKPNAFLTHLKTLAKPPHRRFLSPPPPPPSFVSLRFLSFATPEDAAAERRRRKRRLR 60

Query: 61  IEPPLSSSSAARPLTQPPRSQTPQNPNAPKIPEHISALSGNRLNLHNRILTLIRENDLEE 120
           IEPPLSS    +   Q       +NPNAPK+P+H+SAL+GNRLNLHN+ILTLIRENDLEE
Sbjct: 61  IEPPLSSLHRNQQQQQQSPPPPQRNPNAPKLPDHVSALTGNRLNLHNKILTLIRENDLEE 120

Query: 121 AALFTRHSIYSNCRPTIFTVNAVLNAQLRQSKYADLLSLHRFITQAGVVPNIITHNLIFQ 180
           AAL+TRHSIYSNCRPTIFTVN+VLNA LRQSKY+DLLSLHRFITQAGV PNIITHNL+FQ
Sbjct: 121 AALYTRHSIYSNCRPTIFTVNSVLNALLRQSKYSDLLSLHRFITQAGVAPNIITHNLVFQ 180

Query: 181 TYLDCRKPDTAMEHYKQLINDAPFNPSPTTYRILIKGLVDNNKLERAMELKDEMIEKGFA 240
           TYLDCRKPDTAMEHYKQLINDAPF+PSPTTYRIL+KGLVDNN+LERA+ELK+EM EKG A
Sbjct: 181 TYLDCRKPDTAMEHYKQLINDAPFSPSPTTYRILVKGLVDNNRLERALELKEEMSEKGLA 240

Query: 241 PDPLIYHYLMGGCVRSLDPDGVFKLFEELKEKLGATVEDGVVYGNLMKGYFMKEMEEEAM 300
           PDP +YHYLM GCVR+ D D VF L+EELK KLG  VEDGVVYG+LMK YF+K ME+EAM
Sbjct: 241 PDPTVYHYLMAGCVRNSDVDKVFDLYEELKGKLGGFVEDGVVYGSLMKAYFLKGMEKEAM 300

Query: 301 KCYEETVG---------------------DNSVVKMSAIAYNSVLDALCRNGKFGEALTL 360
           + +EE VG                     +NS VKMSA+AYNSVLDAL +NGKF EAL L
Sbjct: 301 EIFEEAVGAGYFLKGIKKESMETFEEALAENSSVKMSAVAYNSVLDALSKNGKFDEALKL 360

Query: 361 FDRMTKEHRPPRHLAVNLGSFNVMVDGYCIEGRFKEAIEVFEKMGDYRCCPDTLSFNNLI 420
           FDRM KEH PPR LAVNLG+FNV+ +GYC +GRF++AIEVF  MGDYRC PDTLSFN LI
Sbjct: 361 FDRMKKEHNPPRRLAVNLGTFNVIAEGYCAQGRFRDAIEVFRTMGDYRCSPDTLSFNVLI 420

Query: 421 EQLCNNGMLAEAEMLYGTMDDKGVNPDEFTYGLLMDSCFKKNRADDAAAYFRKMVDSGLR 480
           EQLCNNGML EAE LYG M +KGVNPDEFT+GLLMD+CFK+NR DDAA YFRKMVDS LR
Sbjct: 421 EQLCNNGMLGEAEALYGEMGEKGVNPDEFTFGLLMDTCFKENRPDDAAGYFRKMVDSKLR 480

Query: 481 PNIAVYNILVDELVKLGKIDDAKSFFDLMVKKLKMDASSYQFIMKALSESGKMDEILNVV 540
           PN+AVYN LVD LVK+GK+D+AKSFFDLMVKKLKMD  SY+FIMKALSESGK+DE+LNVV
Sbjct: 481 PNLAVYNRLVDGLVKVGKVDEAKSFFDLMVKKLKMDVPSYKFIMKALSESGKLDEVLNVV 540

Query: 541 DTLLDDDGIEFSEELQEFVRGELRKENREEDLAKLVEEKERLKAEAKAKEAEAAEAQKRS 598
           DT+LDDDG+EF+EE+QEFV+GELRKE RE++LAKL+EEKER KAEAKAKEAEAAEA KRS
Sbjct: 541 DTMLDDDGVEFNEEVQEFVKGELRKEGREDELAKLIEEKERQKAEAKAKEAEAAEAAKRS 600

BLAST of Cucsa.032120 vs. TrEMBL
Match: A0A061G122_THECC (Pentatricopeptide repeat-containing protein, putative OS=Theobroma cacao GN=TCM_015413 PE=4 SV=1)

HSP 1 Score: 849.4 bits (2193), Expect = 2.8e-243
Identity = 419/595 (70.42%), Postives = 489/595 (82.18%), Query Frame = 1

Query: 1   MALSKPAFFTHLKTLT-----------------------ADAERRRRKRRLRIEPPLSSS 60
           MALSKP F THL+ L                        A AERRRRKRRLR+EPPLSS+
Sbjct: 1   MALSKPTFLTHLQNLAKRHHRSPPSFITFRHLSFNTPEEAAAERRRRKRRLRVEPPLSSA 60

Query: 61  SAARPLTQPPRSQTP-QNPNAPKIPEHISALSGNRLNLHNRILTLIRENDLEEAALFTRH 120
             ++   Q      P QNPNAPKIPE ++ L+GNRLNLHN+IL LIRENDLEEAAL+TRH
Sbjct: 61  HRSKQQAQQVAPSKPIQNPNAPKIPEPVTVLTGNRLNLHNKILKLIRENDLEEAALYTRH 120

Query: 121 SIYSNCRPTIFTVNAVLNAQLRQSKYADLLSLHRFITQAGVVPNIITHNLIFQTYLDCRK 180
           S+YSNCRPT++TVNAVLNAQLRQSKYADLLSLHRFIT AG+ PN+ITHNLIFQTYLDC+K
Sbjct: 121 SVYSNCRPTVYTVNAVLNAQLRQSKYADLLSLHRFITLAGIAPNVITHNLIFQTYLDCKK 180

Query: 181 PDTAMEHYKQLINDAPFNPSPTTYRILIKGLVDNNKLERAMELKDEMIEKGFAPDPLIYH 240
           PDTA+EHYKQ  N++P NPSPTTYRIL+KGLVDN KLE+A+E+K+EM+EKG APDP++Y 
Sbjct: 181 PDTALEHYKQFSNESPVNPSPTTYRILVKGLVDNGKLEKALEMKEEMVEKGLAPDPVVYS 240

Query: 241 YLMGGCVRSLDPDGVFKLFEELKEKLGATVEDGVVYGNLMKGYFMKEMEEEAMKCYEETV 300
           YL+ GC +S D DG+FKLFEELKEK    +EDGV+YG LMKGYFM+ ME+EAM+CYEE  
Sbjct: 241 YLILGCAKSGDSDGIFKLFEELKEKKDGVLEDGVIYGGLMKGYFMRGMEKEAMECYEEAC 300

Query: 301 GDNSVVKMSAIAYNSVLDALCRNGKFGEALTLFDRMTKEHRPPRHLAVNLGSFNVMVDGY 360
           G+NS VKMSA+AYN VLDAL +NGKF EAL LFDRM  EH PPR LAVNLGSFNV+ DGY
Sbjct: 301 GENSKVKMSAVAYNYVLDALSKNGKFDEALRLFDRMKNEHSPPRRLAVNLGSFNVIADGY 360

Query: 361 CIEGRFKEAIEVFEKMGDYRCCPDTLSFNNLIEQLCNNGMLAEAEMLYGTMDDKGVNPDE 420
           C EG+FKEA+E F  MGDYRC PDTLSFNNLI+QLC NG+L EAE LYG M DKGVNPDE
Sbjct: 361 CAEGKFKEAMEAFRLMGDYRCSPDTLSFNNLIDQLCQNGLLGEAEDLYGEMGDKGVNPDE 420

Query: 421 FTYGLLMDSCFKKNRADDAAAYFRKMVDSGLRPNIAVYNILVDELVKLGKIDDAKSFFDL 480
           +TY LLMD+CFK +R DD A+YFRKMV+SGLRPN+AVYN LVDELVK+GK+D+AKSF+D 
Sbjct: 421 YTYVLLMDACFKVDRIDDGASYFRKMVESGLRPNLAVYNRLVDELVKVGKVDEAKSFYDT 480

Query: 481 MVKKLKMDASSYQFIMKALSESGKMDEILNVVDTLLDDDGIEFSEELQEFVRGELRKENR 540
           MVKKLKMD +SY+F++KALS+ GK+D +L +VD +LDD+ ++F+EELQEFV+ ELR E R
Sbjct: 481 MVKKLKMDDASYKFMIKALSDVGKLDVVLKMVDEMLDDESVDFNEELQEFVKEELRNEGR 540

Query: 541 EEDLAKLVEEKERLKAEAKAKEAEAAEAQKRSAKAAVSSLLSSKLFANKEGEKES 572
           EEDL KL+EEKERLKAEAKA+E EAAEA KRSAKAAVSSLL SKLF  KE E +S
Sbjct: 541 EEDLTKLMEEKERLKAEAKAREIEAAEAAKRSAKAAVSSLLPSKLFGKKEDESQS 595

BLAST of Cucsa.032120 vs. TAIR10
Match: AT3G49240.1 (AT3G49240.1 Pentatricopeptide repeat (PPR) superfamily protein)

HSP 1 Score: 748.4 bits (1931), Expect = 3.4e-216
Identity = 373/594 (62.79%), Postives = 462/594 (77.78%), Query Frame = 1

Query: 1   MALSKPAFFTHLKTLT-------------------------ADAERRRRKRRLRIEPPLS 60
           M++SK AF  HL+TL+                         A AERRRRKRRLR+EPP++
Sbjct: 1   MSISKAAFLNHLQTLSRSYRHRVLPQPFLAVRYMSFATQEEAAAERRRRKRRLRMEPPVN 60

Query: 61  SSSAARPLTQPPRSQTP---QNPNAPKIPEHISALSGNRLNLHNRILTLIRENDLEEAAL 120
           S + +    Q  +SQ P   QNPN PK+PE +SAL G RL+LHN IL LIRENDLEEAAL
Sbjct: 61  SFNRS----QQQQSQIPRPIQNPNIPKLPESVSALVGKRLDLHNHILKLIRENDLEEAAL 120

Query: 121 FTRHSIYSNCRPTIFTVNAVLNAQLRQSKYADLLSLHRFITQAGVVPNIITHNLIFQTYL 180
           +TRHS+YSNCRPTIFTVN VL AQLRQ+KY  LL LH FI QAG+ PNIIT+NLIFQ YL
Sbjct: 121 YTRHSVYSNCRPTIFTVNTVLAAQLRQAKYGALLQLHGFINQAGIAPNIITYNLIFQAYL 180

Query: 181 DCRKPDTAMEHYKQLINDAPFNPSPTTYRILIKGLVDNNKLERAMELKDEMIEKGFAPDP 240
           D RKP+ A+EHYK  I++AP NPS  T+RIL+KGLV N+ LE+AME+K++M  KGF  DP
Sbjct: 181 DVRKPEIALEHYKLFIDNAPLNPSIATFRILVKGLVSNDNLEKAMEIKEDMAVKGFVVDP 240

Query: 241 LIYHYLMGGCVRSLDPDGVFKLFEELKEKLGATVEDGVVYGNLMKGYFMKEMEEEAMKCY 300
           ++Y YLM GCV++ D DGV KL++ELKEKLG  V+DGVVYG LMKGYFMKEME+EAM+CY
Sbjct: 241 VVYSYLMMGCVKNSDADGVLKLYQELKEKLGGFVDDGVVYGQLMKGYFMKEMEKEAMECY 300

Query: 301 EETVGDNSVVKMSAIAYNSVLDALCRNGKFGEALTLFDRMTKEHRPPRHLAVNLGSFNVM 360
           EE VG+NS V+MSA+AYN VL+AL  NGKF EAL LFD + KEH PPRHLAVNLG+FNVM
Sbjct: 301 EEAVGENSKVRMSAMAYNYVLEALSENGKFDEALKLFDAVKKEHNPPRHLAVNLGTFNVM 360

Query: 361 VDGYCIEGRFKEAIEVFEKMGDYRCCPDTLSFNNLIEQLCNNGMLAEAEMLYGTMDDKGV 420
           V+GYC  G+F+EA+EVF +MGD++C PDTLSFNNL+ QLC+N +LAEAE LYG M++K V
Sbjct: 361 VNGYCAGGKFEEAMEVFRQMGDFKCSPDTLSFNNLMNQLCDNELLAEAEKLYGEMEEKNV 420

Query: 421 NPDEFTYGLLMDSCFKKNRADDAAAYFRKMVDSGLRPNIAVYNILVDELVKLGKIDDAKS 480
            PDE+TYGLLMD+CFK+ + D+ AAY++ MV+S LRPN+AVYN L D+L+K GK+DDAKS
Sbjct: 421 KPDEYTYGLLMDTCFKEGKIDEGAAYYKTMVESNLRPNLAVYNRLQDQLIKAGKLDDAKS 480

Query: 481 FFDLMVKKLKMDASSYQFIMKALSESGKMDEILNVVDTLLDDDGIEFSEELQEFVRGELR 540
           FFD+MV KLKMD  +Y+FIM+ALSE+G++DE+L +VD +LDDD +  SEELQEFV+ ELR
Sbjct: 481 FFDMMVSKLKMDDEAYKFIMRALSEAGRLDEMLKIVDEMLDDDTVRVSEELQEFVKEELR 540

Query: 541 KENREEDLAKLVEEKERLKAEAKAKEAEAAEAQKRSAKAAVSSLLSSKLFANKE 567
           K  RE DL KL+EEKERLKAEAKAKE   AE +K++    +++L+  K    K+
Sbjct: 541 KGGREGDLEKLMEEKERLKAEAKAKELADAEEKKKAQSINIAALIPPKAVEEKK 590

BLAST of Cucsa.032120 vs. TAIR10
Match: AT1G10270.1 (AT1G10270.1 glutamine-rich protein 23)

HSP 1 Score: 297.4 bits (760), Expect = 2.1e-80
Identity = 182/519 (35.07%), Postives = 283/519 (54.53%), Query Frame = 1

Query: 17  ADAERRRRKRRLRIEPPLSSSSAARPLTQPPRSQTPQNPNAPKIPEHISALSGNRLNLHN 76
           A AERRRRKRRLRIEPPL +     P   PP+    ++PNAP++P+  SAL G RLNLHN
Sbjct: 96  AAAERRRRKRRLRIEPPLHALRRD-PSAPPPK----RDPNAPRLPDSTSALVGQRLNLHN 155

Query: 77  RILTLIRENDLEEAALFTRHSIYSNCRPTIFTVNAVLNAQLRQSKYADLLSLHR-FITQA 136
           R+ +LIR +DL+ A+   R S++SN RPT+FT NA++ A  R  +Y++ +SL + F  Q+
Sbjct: 156 RVQSLIRASDLDAASKLARQSVFSNTRPTVFTCNAIIAAMYRAKRYSESISLFQYFFKQS 215

Query: 137 GVVPNIITHNLIFQTYLDCRKPDTAMEHYKQLINDAPFNPSPTTYRILIKGLVDNNKLER 196
            +VPN++++N I   + D    D A+E Y+ ++ +APF PS  TYR L KGLV   ++  
Sbjct: 216 NIVPNVVSYNQIINAHCDEGNVDEALEVYRHILANAPFAPSSVTYRHLTKGLVQAGRIGD 275

Query: 197 AMELKDEMIEKGFAPDPLIYHYLMGGCVRSLDPDGVFKLFEELKEKLGATVEDGVVYGNL 256
           A  L  EM+ KG A D  +Y+ L+ G +   D D   + F+ELK K   TV DG+V    
Sbjct: 276 AASLLREMLSKGQAADSTVYNNLIRGYLDLGDFDKAVEFFDELKSK--CTVYDGIVNATF 335

Query: 257 MKGYFMKEMEEEAMKCYEETVGDNSVVKMSAIAYNSVLDALCRNGKFGEALTLFDRMTKE 316
           M+ +F K  ++EAM+ Y   +  +   +M     N +L+   + GK  EA  LF+ M   
Sbjct: 336 MEYWFEKGNDKEAMESYRSLL--DKKFRMHPPTGNVLLEVFLKFGKKDEAWALFNEMLDN 395

Query: 317 HRPPRHLAVNLGSFNVMVDGYCIEGRFKEAIEVFEKMGDYRCCP----DTLSFNNLIEQL 376
           H PP  L+VN  +  +MV+     G F EAI  F+K+G          D L + N++ + 
Sbjct: 396 HAPPNILSVNSDTVGIMVNECFKMGEFSEAINTFKKVGSKVTSKPFVMDYLGYCNIVTRF 455

Query: 377 CNNGMLAEAEMLYGTMDDKGVNPDEFTYGLLMDSCFKKNRADDAAAYFRKMVDSGLRPNI 436
           C  GML EAE  +     + +  D  ++  ++D+  K  R DDA     +MVD  LR   
Sbjct: 456 CEQGMLTEAERFFAEGVSRSLPADAPSHRAMIDAYLKAERIDDAVKMLDRMVDVNLRVVA 515

Query: 437 AVYNILVDELVKLGKIDDAKSFFDLM-VKKLKMDASSYQFIMKALSESGKMDEILNVVDT 496
                +  EL+K GK+ ++      M  ++ K D S Y  +++ L +   +D+  ++V  
Sbjct: 516 DFGARVFGELIKNGKLTESAEVLTKMGEREPKPDPSIYDVVVRGLCDGDALDQAKDIVGE 575

Query: 497 LLDDDGIEFSEELQEFVRGELRKENREEDLAKLVEEKER 530
           ++  + +  +  L+EF+     K  R E++ K++    R
Sbjct: 576 MIRHN-VGVTTVLREFIIEVFEKAGRREEIEKILNSVAR 604

BLAST of Cucsa.032120 vs. TAIR10
Match: AT1G09900.1 (AT1G09900.1 Pentatricopeptide repeat (PPR-like) superfamily protein)

HSP 1 Score: 171.8 bits (434), Expect = 1.3e-42
Identity = 108/412 (26.21%), Postives = 198/412 (48.06%), Query Frame = 1

Query: 75  HNRILTLIRENDLEEAALFTRHSIYSNCRPTIFTVNAVLNAQLRQSKYADLLSLHRFITQ 134
           +N +  ++R  +LEE   F  + +Y    P I     ++    R  K      +   +  
Sbjct: 106 NNHLRQMVRTGELEEGFKFLENMVYHGNVPDIIPCTTLIRGFCRLGKTRKAAKILEILEG 165

Query: 135 AGVVPNIITHNLIFQTYLDCRKPDTAMEHYKQLINDAPFNPSPTTYRILIKGLVDNNKLE 194
           +G VP++IT+N++   Y    + + A+     +++    +P   TY  +++ L D+ KL+
Sbjct: 166 SGAVPDVITYNVMISGYCKAGEINNALS----VLDRMSVSPDVVTYNTILRSLCDSGKLK 225

Query: 195 RAMELKDEMIEKGFAPDPLIYHYLMGGCVRSLDPDGVFKLFEELKEKLGATVEDGVVYGN 254
           +AME+ D M+++   PD + Y  L+    R        KL +E++++ G T  D V Y  
Sbjct: 226 QAMEVLDRMLQRDCYPDVITYTILIEATCRDSGVGHAMKLLDEMRDR-GCT-PDVVTYNV 285

Query: 255 LMKGYFMKEMEEEAMKCYEETVGDNSVVKMSAIAYNSVLDALCRNGKFGEALTLFDRMTK 314
           L+ G   +   +EA+K   +    +S  + + I +N +L ++C  G++ +A  L   M +
Sbjct: 286 LVNGICKEGRLDEAIKFLNDM--PSSGCQPNVITHNIILRSMCSTGRWMDAEKLLADMLR 345

Query: 315 EHRPPRHLAVNLGSFNVMVDGYCIEGRFKEAIEVFEKMGDYRCCPDTLSFNNLIEQLCNN 374
           +   P     ++ +FN++++  C +G    AI++ EKM  + C P++LS+N L+   C  
Sbjct: 346 KGFSP-----SVVTFNILINFLCRKGLLGRAIDILEKMPQHGCQPNSLSYNPLLHGFCKE 405

Query: 375 GMLAEAEMLYGTMDDKGVNPDEFTYGLLMDSCFKKNRADDAAAYFRKMVDSGLRPNIAVY 434
             +  A      M  +G  PD  TY  ++ +  K  + +DA     ++   G  P +  Y
Sbjct: 406 KKMDRAIEYLERMVSRGCYPDIVTYNTMLTALCKDGKVEDAVEILNQLSSKGCSPVLITY 465

Query: 435 NILVDELVKLGKIDDAKSFFDLM-VKKLKMDASSYQFIMKALSESGKMDEIL 486
           N ++D L K GK   A    D M  K LK D  +Y  ++  LS  GK+DE +
Sbjct: 466 NTVIDGLAKAGKTGKAIKLLDEMRAKDLKPDTITYSSLVGGLSREGKVDEAI 504


HSP 2 Score: 122.5 bits (306), Expect = 9.1e-28
Identity = 88/397 (22.17%), Postives = 185/397 (46.60%), Query Frame = 1

Query: 75  HNRIL-TLIRENDLEEAALFTRHSIYSNCRPTIFTVNAVLNAQLRQSKYADLLSLHRFIT 134
           +N IL +L     L++A       +  +C P + T   ++ A  R S     + L   + 
Sbjct: 207 YNTILRSLCDSGKLKQAMEVLDRMLQRDCYPDVITYTILIEATCRDSGVGHAMKLLDEMR 266

Query: 135 QAGVVPNIITHNLIFQTYLDCRKPDTAMEHYKQLINDAPFN---PSPTTYRILIKGLVDN 194
             G  P+++T+N++        + D A++     +ND P +   P+  T+ I+++ +   
Sbjct: 267 DRGCTPDVVTYNVLVNGICKEGRLDEAIK----FLNDMPSSGCQPNVITHNIILRSMCST 326

Query: 195 NKLERAMELKDEMIEKGFAPDPLIYHYLMGGCVRSLDPDGVFKLFEELKEKLGATVEDGV 254
            +   A +L  +M+ KGF+P  + ++ L+    R         + E++ +       + +
Sbjct: 327 GRWMDAEKLLADMLRKGFSPSVVTFNILINFLCRKGLLGRAIDILEKMPQH--GCQPNSL 386

Query: 255 VYGNLMKGYFMKEMEEEAMKCYEETVGDNSVVKMSAIAYNSVLDALCRNGKFGEALTLFD 314
            Y  L+ G+  ++  + A++  E  V       +  + YN++L ALC++GK  +A+ + +
Sbjct: 387 SYNPLLHGFCKEKKMDRAIEYLERMVSRGCYPDI--VTYNTMLTALCKDGKVEDAVEILN 446

Query: 315 RMTKEHRPPRHLAVNLGSFNVMVDGYCIEGRFKEAIEVFEKMGDYRCCPDTLSFNNLIEQ 374
           +++ +   P      L ++N ++DG    G+  +AI++ ++M      PDT+++++L+  
Sbjct: 447 QLSSKGCSPV-----LITYNTVIDGLAKAGKTGKAIKLLDEMRAKDLKPDTITYSSLVGG 506

Query: 375 LCNNGMLAEAEMLYGTMDDKGVNPDEFTYGLLMDSCFKKNRADDAAAYFRKMVDSGLRPN 434
           L   G + EA   +   +  G+ P+  T+  +M    K  + D A  +   M++ G +PN
Sbjct: 507 LSREGKVDEAIKFFHEFERMGIRPNAVTFNSIMLGLCKSRQTDRAIDFLVFMINRGCKPN 566

Query: 435 IAVYNILVDELVKLGKIDDAKSFFDLMVKKLKMDASS 468
              Y IL++ L   G   +A    + +  K  M  SS
Sbjct: 567 ETSYTILIEGLAYEGMAKEALELLNELCNKGLMKKSS 590


HSP 3 Score: 116.3 bits (290), Expect = 6.6e-26
Identity = 97/402 (24.13%), Postives = 178/402 (44.28%), Query Frame = 1

Query: 136 GVVPNIITHNLIFQTYLDCRKPDTAMEHYKQLINDAPFNPSPTTYRILIKGLVDNNKLER 195
           G VP+II    + + +    K   A +   +++  +   P   TY ++I G     ++  
Sbjct: 132 GNVPDIIPCTTLIRGFCRLGKTRKAAK-ILEILEGSGAVPDVITYNVMISGYCKAGEINN 191

Query: 196 AMELKDEMIEKGFAPDPLIYHYLMGGCVRSLDPDGVFKLFEELKEKL--GATVEDGVVYG 255
           A+ + D M     +PD + Y+ ++    RSL   G  K   E+ +++       D + Y 
Sbjct: 192 ALSVLDRM---SVSPDVVTYNTIL----RSLCDSGKLKQAMEVLDRMLQRDCYPDVITYT 251

Query: 256 NLMKGYFMKEMEEEAMKCYEETVGDNSVVKMSAIAYNSVLDALCRNGKFGEALTLFDRMT 315
            L++          AMK  +E + D        + YN +++ +C+ G+  EA+   + M 
Sbjct: 252 ILIEATCRDSGVGHAMKLLDE-MRDRGCTP-DVVTYNVLVNGICKEGRLDEAIKFLNDMP 311

Query: 316 KEHRPPRHLAVNLGSFNVMVDGYCIEGRFKEAIEVFEKMGDYRCCPDTLSFNNLIEQLCN 375
                P     N+ + N+++   C  GR+ +A ++   M      P  ++FN LI  LC 
Sbjct: 312 SSGCQP-----NVITHNIILRSMCSTGRWMDAEKLLADMLRKGFSPSVVTFNILINFLCR 371

Query: 376 NGMLAEAEMLYGTMDDKGVNPDEFTYGLLMDSCFKKNRADDAAAYFRKMVDSGLRPNIAV 435
            G+L  A  +   M   G  P+  +Y  L+    K+ + D A  Y  +MV  G  P+I  
Sbjct: 372 KGLLGRAIDILEKMPQHGCQPNSLSYNPLLHGFCKEKKMDRAIEYLERMVSRGCYPDIVT 431

Query: 436 YNILVDELVKLGKIDDAKSFFD-LMVKKLKMDASSYQFIMKALSESGKMDEILNVVDTL- 495
           YN ++  L K GK++DA    + L  K       +Y  ++  L+++GK  + + ++D + 
Sbjct: 432 YNTMLTALCKDGKVEDAVEILNQLSSKGCSPVLITYNTVIDGLAKAGKTGKAIKLLDEMR 491

Query: 496 ---LDDDGIEFSEELQEFVRGELRKENREEDLAKLVEEKERL 531
              L  D I +S      + G L +E + ++  K   E ER+
Sbjct: 492 AKDLKPDTITYSS-----LVGGLSREGKVDEAIKFFHEFERM 513

BLAST of Cucsa.032120 vs. TAIR10
Match: AT1G12300.1 (AT1G12300.1 Tetratricopeptide repeat (TPR)-like superfamily protein)

HSP 1 Score: 165.2 bits (417), Expect = 1.2e-40
Identity = 109/419 (26.01%), Postives = 199/419 (47.49%), Query Frame = 1

Query: 65  SALSGNRLNLHNRILTLIRENDLEEAALFTRHSIYSNCRPTIFTVNAVLNAQLRQSKYAD 124
           SA S   L+   R+ + + +   ++A    R  I+S   PT+   + + +A  +  +Y  
Sbjct: 47  SAFSDRNLSYRERLRSGLVDIKADDAIDLFRDMIHSRPLPTVIDFSRLFSAIAKTKQYDL 106

Query: 125 LLSLHRFITQAGVVPNIITHNLIFQTYLDCRKPDTAMEHYKQLINDAPFNPSPTTYRILI 184
           +L+L + +   G+  N+ T +++   +  CRK   A     ++I    + P+  T+  LI
Sbjct: 107 VLALCKQMELKGIAHNLYTLSIMINCFCRCRKLCLAFSAMGKIIK-LGYEPNTITFSTLI 166

Query: 185 KGLVDNNKLERAMELKDEMIEKGFAPDPLIYHYLMGGCVRSLDPDGVFKLFEELKEKLGA 244
            GL    ++  A+EL D M+E G  PD +  + L+ G   S        L +++ E    
Sbjct: 167 NGLCLEGRVSEALELVDRMVEMGHKPDLITINTLVNGLCLSGKEAEAMLLIDKMVEY--G 226

Query: 245 TVEDGVVYGNLMKGYFMKEMEEEAMKCYEETVGDNSVVKMSAIAYNSVLDALCRNGKFGE 304
              + V YG ++           AM+   +    N  +K+ A+ Y+ ++D LC++G    
Sbjct: 227 CQPNAVTYGPVLNVMCKSGQTALAMELLRKMEERN--IKLDAVKYSIIIDGLCKHGSLDN 286

Query: 305 ALTLFDRMTKEHRPPRHLAVNLGSFNVMVDGYCIEGRFKEAIEVFEKMGDYRCCPDTLSF 364
           A  LF+ M       + +  N+ ++N+++ G+C  GR+ +  ++   M   +  P+ ++F
Sbjct: 287 AFNLFNEMEM-----KGITTNIITYNILIGGFCNAGRWDDGAKLLRDMIKRKINPNVVTF 346

Query: 365 NNLIEQLCNNGMLAEAEMLYGTMDDKGVNPDEFTYGLLMDSCFKKNRADDAAAYFRKMVD 424
           + LI+     G L EAE L+  M  +G+ PD  TY  L+D   K+N  D A      MV 
Sbjct: 347 SVLIDSFVKEGKLREAEELHKEMIHRGIAPDTITYTSLIDGFCKENHLDKANQMVDLMVS 406

Query: 425 SGLRPNIAVYNILVDELVKLGKIDDAKSFFDLM-VKKLKMDASSYQFIMKALSESGKMD 483
            G  PNI  +NIL++   K  +IDD    F  M ++ +  D  +Y  +++   E GK++
Sbjct: 407 KGCDPNIRTFNILINGYCKANRIDDGLELFRKMSLRGVVADTVTYNTLIQGFCELGKLN 455


HSP 2 Score: 154.8 bits (390), Expect = 1.7e-37
Identity = 113/462 (24.46%), Postives = 205/462 (44.37%), Query Frame = 1

Query: 70  NRLNLHNRILTLIRENDLEEAALFTRHSIYSNCRPTIFTVNAVLNAQLRQSKYADLLSLH 129
           N +     I  L  E  + EA       +    +P + T+N ++N      K A+ + L 
Sbjct: 157 NTITFSTLINGLCLEGRVSEALELVDRMVEMGHKPDLITINTLVNGLCLSGKEAEAMLLI 216

Query: 130 RFITQAGVVPNIITHNLIFQTYLDCRKPDTAMEHYKQLINDAPFNPSPTTYRILIKGLVD 189
             + + G  PN +T+  +        +   AME  +++  +         Y I+I GL  
Sbjct: 217 DKMVEYGCQPNAVTYGPVLNVMCKSGQTALAMELLRKM-EERNIKLDAVKYSIIIDGLCK 276

Query: 190 NNKLERAMELKDEMIEKGFAPDPLIYHYLMGG-CVRSLDPDGVFKLFEELKEKLGATVED 249
           +  L+ A  L +EM  KG   + + Y+ L+GG C      DG   L + +K K+   V  
Sbjct: 277 HGSLDNAFNLFNEMEMKGITTNIITYNILIGGFCNAGRWDDGAKLLRDMIKRKINPNV-- 336

Query: 250 GVVYGNLMKGYFMKEMEEEAMKCYEETVGDNSVVKMSAIAYNSVLDALCRNGKFGEALTL 309
            V +  L+  +  +    EA + ++E +  +  +    I Y S++D  C+     +A  +
Sbjct: 337 -VTFSVLIDSFVKEGKLREAEELHKEMI--HRGIAPDTITYTSLIDGFCKENHLDKANQM 396

Query: 310 FDRMTKEHRPPRHLAVNLGSFNVMVDGYCIEGRFKEAIEVFEKMGDYRCCPDTLSFNNLI 369
            D M  +   P     N+ +FN++++GYC   R  + +E+F KM       DT+++N LI
Sbjct: 397 VDLMVSKGCDP-----NIRTFNILINGYCKANRIDDGLELFRKMSLRGVVADTVTYNTLI 456

Query: 370 EQLCNNGMLAEAEMLYGTMDDKGVNPDEFTYGLLMDSCFKKNRADDAAAYFRKMVDSGLR 429
           +  C  G L  A+ L+  M  + V P+  TY +L+D       ++ A   F K+  S + 
Sbjct: 457 QGFCELGKLNVAKELFQEMVSRKVPPNIVTYKILLDGLCDNGESEKALEIFEKIEKSKME 516

Query: 430 PNIAVYNILVDELVKLGKIDDAKSFF-DLMVKKLKMDASSYQFIMKALSESGKMDEILNV 489
            +I +YNI++  +    K+DDA   F  L +K +K    +Y  ++  L + G + E   +
Sbjct: 517 LDIGIYNIIIHGMCNASKVDDAWDLFCSLPLKGVKPGVKTYNIMIGGLCKKGPLSEAELL 576

Query: 490 VDTLLDDDGIEFSEELQEFVRGELRKENREEDLAKLVEEKER 530
              + +D            +R  L   +  + + KL+EE +R
Sbjct: 577 FRKMEEDGHAPDGWTYNILIRAHLGDGDATKSV-KLIEELKR 606


HSP 3 Score: 110.5 bits (275), Expect = 3.6e-24
Identity = 89/362 (24.59%), Postives = 174/362 (48.07%), Query Frame = 1

Query: 173 FNPSPTTYRILIK-GLVDNNKLERAMELKDEMIEKGFAPDPLIYHYLMGGCVRSLDPDGV 232
           F+    +YR  ++ GLVD  K + A++L  +MI     P  + +  L     ++   D V
Sbjct: 49  FSDRNLSYRERLRSGLVDI-KADDAIDLFRDMIHSRPLPTVIDFSRLFSAIAKTKQYDLV 108

Query: 233 FKLFEELKEKLGATVEDGVVYGNLMKGYFMKEMEEEAMKCYEETVGDNSVVKM------- 292
             L ++++ K       G+ + NL   Y +  M     +C +  +  +++ K+       
Sbjct: 109 LALCKQMELK-------GIAH-NL---YTLSIMINCFCRCRKLCLAFSAMGKIIKLGYEP 168

Query: 293 SAIAYNSVLDALCRNGKFGEALTLFDRMTKEHRPPRHLAVNLGSFNVMVDGYCIEGRFKE 352
           + I ++++++ LC  G+  EAL L DRM +    P     +L + N +V+G C+ G+  E
Sbjct: 169 NTITFSTLINGLCLEGRVSEALELVDRMVEMGHKP-----DLITINTLVNGLCLSGKEAE 228

Query: 353 AIEVFEKMGDYRCCPDTLSFNNLIEQLCNNGMLAEAEMLYGTMDDKGVNPDEFTYGLLMD 412
           A+ + +KM +Y C P+ +++  ++  +C +G  A A  L   M+++ +  D   Y +++D
Sbjct: 229 AMLLIDKMVEYGCQPNAVTYGPVLNVMCKSGQTALAMELLRKMEERNIKLDAVKYSIIID 288

Query: 413 SCFKKNRADDAAAYFRKMVDSGLRPNIAVYNILVDELVKLGKIDD-AKSFFDLMVKKLKM 472
              K    D+A   F +M   G+  NI  YNIL+      G+ DD AK   D++ +K+  
Sbjct: 289 GLCKHGSLDNAFNLFNEMEMKGITTNIITYNILIGGFCNAGRWDDGAKLLRDMIKRKINP 348

Query: 473 DASSYQFIMKALSESGKMDEI----LNVVDTLLDDDGIEFSEELQEFVR-GELRKENREE 521
           +  ++  ++ +  + GK+ E       ++   +  D I ++  +  F +   L K N+  
Sbjct: 349 NVVTFSVLIDSFVKEGKLREAEELHKEMIHRGIAPDTITYTSLIDGFCKENHLDKANQMV 393

BLAST of Cucsa.032120 vs. TAIR10
Match: AT1G63070.1 (AT1G63070.1 pentatricopeptide (PPR) repeat-containing protein)

HSP 1 Score: 159.5 bits (402), Expect = 6.7e-39
Identity = 107/425 (25.18%), Postives = 210/425 (49.41%), Query Frame = 1

Query: 106 IFTVNAVLNAQLRQSKYADLLSLHRFITQAGVVPNIITHNLIFQTYLDCRKPDTAMEHYK 165
           ++T +  +N   R+S+ +  L++   + + G  P+I+T N +   +    +   A+    
Sbjct: 110 LYTYSIFINYFCRRSQLSLALAILGKMMKLGYGPSIVTLNSLLNGFCHGNRISEAVALVD 169

Query: 166 QLINDAPFNPSPTTYRILIKGLVDNNKLERAMELKDEMIEKGFAPDPLIYHYLMGGCVRS 225
           Q++ +  + P   T+  L+ GL  +NK   A+ L + M+ KG  PD + Y  ++ G  + 
Sbjct: 170 QMV-EMGYQPDTVTFTTLVHGLFQHNKASEAVALVERMVVKGCQPDLVTYGAVINGLCKR 229

Query: 226 LDPDGVFKLFEELKEKLGATVEDGVVYGNLMKGYFMKEMEEEAMKCYEETVGDNSVVKMS 285
            +PD    L  ++++  G    D V+Y  ++ G    +  ++A   + +   +   +K  
Sbjct: 230 GEPDLALNLLNKMEK--GKIEADVVIYNTIIDGLCKYKHMDDAFDLFNKM--ETKGIKPD 289

Query: 286 AIAYNSVLDALCRNGKFGEALTLFDRMTKEHRPPRHLAVNLGSFNVMVDGYCIEGRFKEA 345
              YN ++  LC  G++ +A  L   M +++  P     +L  FN ++D +  EG+  EA
Sbjct: 290 VFTYNPLISCLCNYGRWSDASRLLSDMLEKNINP-----DLVFFNALIDAFVKEGKLVEA 349

Query: 346 IEVFEKMGDYR-CCPDTLSFNNLIEQLCNNGMLAEAEMLYGTMDDKGVNPDEFTYGLLMD 405
            +++++M   + C PD +++N LI+  C    + E   ++  M  +G+  +  TY  L+ 
Sbjct: 350 EKLYDEMVKSKHCFPDVVAYNTLIKGFCKYKRVEEGMEVFREMSQRGLVGNTVTYTTLIH 409

Query: 406 SCFKKNRADDAAAYFRKMVDSGLRPNIAVYNILVDELVKLGKIDDAKSFFDLMVKK-LKM 465
             F+    D+A   F++MV  G+ P+I  YNIL+D L   G ++ A   F+ M K+ +K+
Sbjct: 410 GFFQARDCDNAQMVFKQMVSDGVHPDIMTYNILLDGLCNNGNVETALVVFEYMQKRDMKL 469

Query: 466 DASSYQFIMKALSESGKMDEILNVVDTLLDDDGIEFSEELQEFVRGELRKENREEDLAKL 525
           D  +Y  +++AL ++GK+++  ++  +L               + G  RK  +EE  A  
Sbjct: 470 DIVTYTTMIEALCKAGKVEDGWDLFCSLSLKGVKPNVVTYTTMMSGFCRKGLKEEADALF 524

Query: 526 VEEKE 529
           VE KE
Sbjct: 530 VEMKE 524


HSP 2 Score: 130.2 bits (326), Expect = 4.4e-30
Identity = 102/428 (23.83%), Postives = 190/428 (44.39%), Query Frame = 1

Query: 71  RLNLHNRILTLIRENDLEEAALFTRHSIYSNCRPTIFTVNAVLNAQLRQSKYADLLSLHR 130
           R NL  ++L  ++   L++A       + S   P+I   + +L+A  + +K+  ++SL  
Sbjct: 43  RENLSRKVLQDLK---LDDAIGLFGDMVKSRPFPSIVEFSKLLSAIAKMNKFDLVISLGE 102

Query: 131 FITQAGVVPNIITHNLIFQTYLDCRKPDTAMEHYKQLINDAPFNPSPTTYRILIKGLVDN 190
            +   G+  N+ T+++    +    +   A+    +++    + PS  T   L+ G    
Sbjct: 103 QMQNLGISHNLYTYSIFINYFCRRSQLSLALAILGKMMK-LGYGPSIVTLNSLLNGFCHG 162

Query: 191 NKLERAMELKDEMIEKGFAPDPLIYHYLMGGCVRSLDPDGVFKLFEELKEKLGATVEDGV 250
           N++  A+ L D+M+E G+ PD + +  L+ G  +         L E +  K      D V
Sbjct: 163 NRISEAVALVDQMVEMGYQPDTVTFTTLVHGLFQHNKASEAVALVERMVVK--GCQPDLV 222

Query: 251 VYGNLMKGYFMKEMEEEAMKCYEETVGDNSVVKMSAIAYNSVLDALCRNGKFGEALTLFD 310
            YG ++ G   +   + A+    +   +   ++   + YN+++D LC+     +A  LF+
Sbjct: 223 TYGAVINGLCKRGEPDLALNLLNKM--EKGKIEADVVIYNTIIDGLCKYKHMDDAFDLFN 282

Query: 311 RM-TKEHRPPRHLAVNLGSFNVMVDGYCIEGRFKEAIEVFEKMGDYRCCPDTLSFNNLIE 370
           +M TK  +P      ++ ++N ++   C  GR+ +A  +   M +    PD + FN LI+
Sbjct: 283 KMETKGIKP------DVFTYNPLISCLCNYGRWSDASRLLSDMLEKNINPDLVFFNALID 342

Query: 371 QLCNNGMLAEAEMLYGTM-DDKGVNPDEFTYGLLMDSCFKKNRADDAAAYFRKMVDSGLR 430
                G L EAE LY  M   K   PD   Y  L+    K  R ++    FR+M   GL 
Sbjct: 343 AFVKEGKLVEAEKLYDEMVKSKHCFPDVVAYNTLIKGFCKYKRVEEGMEVFREMSQRGLV 402

Query: 431 PNIAVYNILVDELVKLGKIDDAKSFFDLMVKK-LKMDASSYQFIMKALSESGKMDEILNV 490
            N   Y  L+    +    D+A+  F  MV   +  D  +Y  ++  L  +G ++  L V
Sbjct: 403 GNTVTYTTLIHGFFQARDCDNAQMVFKQMVSDGVHPDIMTYNILLDGLCNNGNVETALVV 456

Query: 491 VDTLLDDD 496
            + +   D
Sbjct: 463 FEYMQKRD 456


HSP 3 Score: 124.0 bits (310), Expect = 3.1e-28
Identity = 97/408 (23.77%), Postives = 187/408 (45.83%), Query Frame = 1

Query: 81  LIRENDLEEAALFTRHSIYSNCRPTIFTVNAVLNAQLRQSKYADLLSLHRFITQAGVVPN 140
           L + N   EA       +   C+P + T  AV+N   ++ +    L+L   + +  +  +
Sbjct: 190 LFQHNKASEAVALVERMVVKGCQPDLVTYGAVINGLCKRGEPDLALNLLNKMEKGKIEAD 249

Query: 141 IITHNLIFQTYLDCRKPDTAMEHYKQLINDAPFNPSPTTYRILIKGLVDNNKLERAMELK 200
           ++ +N I       +  D A + + ++       P   TY  LI  L +  +   A  L 
Sbjct: 250 VVIYNTIIDGLCKYKHMDDAFDLFNKMETKG-IKPDVFTYNPLISCLCNYGRWSDASRLL 309

Query: 201 DEMIEKGFAPDPLIYHYLMGGCVRSLDPDGVFKLFEELKEKLGATVEDGVVYGNLMKGYF 260
            +M+EK   PD + ++ L+   V+        KL++E+  K      D V Y  L+KG+ 
Sbjct: 310 SDMLEKNINPDLVFFNALIDAFVKEGKLVEAEKLYDEMV-KSKHCFPDVVAYNTLIKGFC 369

Query: 261 MKEMEEEAMKCYEETVGDNSVVKMSAIAYNSVLDALCRNGKFGEALTLFDRMTKEHRPPR 320
             +  EE M+ + E +    +V  + + Y +++    +      A  +F +M  +   P 
Sbjct: 370 KYKRVEEGMEVFRE-MSQRGLVG-NTVTYTTLIHGFFQARDCDNAQMVFKQMVSDGVHP- 429

Query: 321 HLAVNLGSFNVMVDGYCIEGRFKEAIEVFEKMGDYRCCPDTLSFNNLIEQLCNNGMLAEA 380
               ++ ++N+++DG C  G  + A+ VFE M       D +++  +IE LC  G + + 
Sbjct: 430 ----DIMTYNILLDGLCNNGNVETALVVFEYMQKRDMKLDIVTYTTMIEALCKAGKVEDG 489

Query: 381 EMLYGTMDDKGVNPDEFTYGLLMDSCFKKNRADDAAAYFRKMVDSGLRPNIAVYNILVDE 440
             L+ ++  KGV P+  TY  +M    +K   ++A A F +M + G  PN   YN L+  
Sbjct: 490 WDLFCSLSLKGVKPNVVTYTTMMSGFCRKGLKEEADALFVEMKEDGPLPNSGTYNTLIRA 549

Query: 441 LVKLGKIDDAKSFFDLMVKKLKM-----DASSYQFIMKALSESGKMDE 484
            ++ G  D+A S    ++K+++      DAS++  +   L + G++D+
Sbjct: 550 RLRDG--DEAAS--AELIKEMRSCGFAGDASTFGLVTNMLHD-GRLDK 583

BLAST of Cucsa.032120 vs. NCBI nr
Match: gi|449456969|ref|XP_004146221.1| (PREDICTED: pentatricopeptide repeat-containing protein At3g49240 [Cucumis sativus])

HSP 1 Score: 1120.5 bits (2897), Expect = 0.0e+00
Identity = 573/611 (93.78%), Postives = 575/611 (94.11%), Query Frame = 1

Query: 1   MALSKPAFFTHLKTLT-----------------------------ADAERRRRKRRLRIE 60
           MALSKPAFFTHLKTLT                             ADAERRRRKRRLRIE
Sbjct: 1   MALSKPAFFTHLKTLTGSHHLLQRQALAPFPIVTLRFLSFASAEEADAERRRRKRRLRIE 60

Query: 61  PPLSSSSAARPLTQPPRSQTPQNPNAPKIPEHISALSGNRLNLHNRILTLIRENDLEEAA 120
           PPLSSSSAARPLTQPPRSQTPQNPNAPKIPEHISALSGNRLNLHNRILTLIRENDLEEAA
Sbjct: 61  PPLSSSSAARPLTQPPRSQTPQNPNAPKIPEHISALSGNRLNLHNRILTLIRENDLEEAA 120

Query: 121 LFTRHSIYSNCRPTIFTVNAVLNAQLRQSKYADLLSLHRFITQAGVVPNIITHNLIFQTY 180
           LFTRHSIYSNCRPTIFTVNAVLNAQLRQSKYADLLSLHRFITQAGVVPNIITHNLIFQTY
Sbjct: 121 LFTRHSIYSNCRPTIFTVNAVLNAQLRQSKYADLLSLHRFITQAGVVPNIITHNLIFQTY 180

Query: 181 LDCRKPDTAMEHYKQLINDAPFNPSPTTYRILIKGLVDNNKLERAMELKDEMIEKGFAPD 240
           LDCRKPDTAMEHYKQLINDAPFNPSPTTYRILIKGLVDNNKLERAMELKDEMIEKGFAPD
Sbjct: 181 LDCRKPDTAMEHYKQLINDAPFNPSPTTYRILIKGLVDNNKLERAMELKDEMIEKGFAPD 240

Query: 241 PLIYHYLMGGCVRSLDPDGVFKLFEELKEKLGATVEDGVVYGNLMKGYFMKEMEEEAMKC 300
           PLIYHYLMGGCVRSLDPDGVFKLFEELKEKLGATVEDGVVYGNLMKGYFMKEMEEEAMKC
Sbjct: 241 PLIYHYLMGGCVRSLDPDGVFKLFEELKEKLGATVEDGVVYGNLMKGYFMKEMEEEAMKC 300

Query: 301 YEETVGDNSVVKMSAIAYNSVLDALCRNGKFGEALTLFDRMTKEHRPPRHLAVNLGSFNV 360
           YEETVGDNSVVKMSAIAYNSVLDALCRNGKFGEALTLFDRMTKEHRPPRHLAVNLGSFNV
Sbjct: 301 YEETVGDNSVVKMSAIAYNSVLDALCRNGKFGEALTLFDRMTKEHRPPRHLAVNLGSFNV 360

Query: 361 MVDGYCIEGRFKEAIEVFEKMGDYRCCPDTLSFNNLIEQLCNNGMLAEAEMLYGTMDDKG 420
           MVDGYCIEGRFKEAIEVFEKMGDYRCCPDTLSFNNLIEQLCNNGMLAEAEMLYGTMDDKG
Sbjct: 361 MVDGYCIEGRFKEAIEVFEKMGDYRCCPDTLSFNNLIEQLCNNGMLAEAEMLYGTMDDKG 420

Query: 421 VNPDEFTYGLLMDSCFKKNRADDAAAYFRKMVDSGLRPNIAVYNILVDELVKLGKIDDAK 480
           VNPDEFTYGLLMDSCFKKNRADDAAAYFRKMVDSGLRPNIAVYNILVDELVKLGKIDDAK
Sbjct: 421 VNPDEFTYGLLMDSCFKKNRADDAAAYFRKMVDSGLRPNIAVYNILVDELVKLGKIDDAK 480

Query: 481 SFFDLMVKKLKMDASSYQFIMKALSESGKMDEILNVVDTLLDDDGIEFSEELQEFVRGEL 540
           SFFDLMVKKLKMDASSYQFIMKALSESGKMDEILNVVDTLLDDDGIEFSEELQEFVRGEL
Sbjct: 481 SFFDLMVKKLKMDASSYQFIMKALSESGKMDEILNVVDTLLDDDGIEFSEELQEFVRGEL 540

Query: 541 RKENREEDLAKLVEEKERLKAEAKAKEAEAAEAQKRSAKAAVSSLLSSKLFANKEGEKES 583
           RKENREEDLAKLVEEKERLKAEAKAKEAEAAEAQKRSAKAAVSSLLSSKLFANKEGEKES
Sbjct: 541 RKENREEDLAKLVEEKERLKAEAKAKEAEAAEAQKRSAKAAVSSLLSSKLFANKEGEKES 600

BLAST of Cucsa.032120 vs. NCBI nr
Match: gi|659133624|ref|XP_008466825.1| (PREDICTED: pentatricopeptide repeat-containing protein At3g49240 [Cucumis melo])

HSP 1 Score: 1063.9 bits (2750), Expect = 1.0e-307
Identity = 542/601 (90.18%), Postives = 557/601 (92.68%), Query Frame = 1

Query: 1   MALSKPAFFTHLKTLT-----------------------------ADAERRRRKRRLRIE 60
           MALSKPAFFTHLKTLT                             ADAERRRRKRRLRIE
Sbjct: 1   MALSKPAFFTHLKTLTGSHHLLQRQAPAPLPIVTFRFLSFASAEEADAERRRRKRRLRIE 60

Query: 61  PPLSSSSAARPLTQPPRSQTPQNPNAPKIPEHISALSGNRLNLHNRILTLIRENDLEEAA 120
           PPLSSSSAARP +QP RSQTPQNPN PK+PEHISALSGNRLNLHNRILTLIRENDLEEAA
Sbjct: 61  PPLSSSSAARPQSQPSRSQTPQNPNTPKVPEHISALSGNRLNLHNRILTLIRENDLEEAA 120

Query: 121 LFTRHSIYSNCRPTIFTVNAVLNAQLRQSKYADLLSLHRFITQAGVVPNIITHNLIFQTY 180
           LFTRHSIYSNCRPTIFTVNAVLNAQLRQSKYADLLSLHRFITQAGVVPNIITHNLIFQTY
Sbjct: 121 LFTRHSIYSNCRPTIFTVNAVLNAQLRQSKYADLLSLHRFITQAGVVPNIITHNLIFQTY 180

Query: 181 LDCRKPDTAMEHYKQLINDAPFNPSPTTYRILIKGLVDNNKLERAMELKDEMIEKGFAPD 240
           LDCRKPDTAMEHYKQLINDAPFNPSPTTYRILIKGLVDN KLERAMELK+EMI KGFAPD
Sbjct: 181 LDCRKPDTAMEHYKQLINDAPFNPSPTTYRILIKGLVDNKKLERAMELKEEMIVKGFAPD 240

Query: 241 PLIYHYLMGGCVRSLDPDGVFKLFEELKEKLGATVEDGVVYGNLMKGYFMKEMEEEAMKC 300
           PLIYHYLM GCVRS DPDGVFKLFEELKEKLG TVEDGVVYGNLMKGYFMKEMEEEAMKC
Sbjct: 241 PLIYHYLMAGCVRSSDPDGVFKLFEELKEKLGGTVEDGVVYGNLMKGYFMKEMEEEAMKC 300

Query: 301 YEETVGDNSVVKMSAIAYNSVLDALCRNGKFGEALTLFDRMTKEHRPPRHLAVNLGSFNV 360
           YEETVGDN VVKMSAIAYNSVLDALC++GKF EALTLFDRMTKEHRPPRHLAVNLG+FNV
Sbjct: 301 YEETVGDNPVVKMSAIAYNSVLDALCKHGKFSEALTLFDRMTKEHRPPRHLAVNLGTFNV 360

Query: 361 MVDGYCIEGRFKEAIEVFEKMGDYRCCPDTLSFNNLIEQLCNNGMLAEAEMLYGTMDDKG 420
           MVDGYCI+GRFKEAI VFE+MGDYRC PDTLSFNNLIEQLCNNGMLAEAEMLYGTM +KG
Sbjct: 361 MVDGYCIKGRFKEAIGVFEEMGDYRCSPDTLSFNNLIEQLCNNGMLAEAEMLYGTMGEKG 420

Query: 421 VNPDEFTYGLLMDSCFKKNRADDAAAYFRKMVDSGLRPNIAVYNILVDELVKLGKIDDAK 480
           VNPDEFTYGLLM SCF+KNRADDAAAYFRKMVDSGLRPNIAVYNILV ELVKLGK+D+AK
Sbjct: 421 VNPDEFTYGLLMHSCFQKNRADDAAAYFRKMVDSGLRPNIAVYNILVGELVKLGKVDEAK 480

Query: 481 SFFDLMVKKLKMDASSYQFIMKALSESGKMDEILNVVDTLLDDDGIEFSEELQEFVRGEL 540
           SFFDLMVKKLKMDAS+YQFIMKALSESGKMDE+LNVVDTLLDDDGIEFSEELQEFVRGEL
Sbjct: 481 SFFDLMVKKLKMDASNYQFIMKALSESGKMDEVLNVVDTLLDDDGIEFSEELQEFVRGEL 540

Query: 541 RKENREEDLAKLVEEKERLKAEAKAKEAEAAEAQKRSAKAAVSSLLSSKLFANKEGEKES 573
           RKE+REEDLAKLVEEKERLKAEAKAKEAEAAEAQKRSAKAAVSSLLSSKLFANKEGEKES
Sbjct: 541 RKEDREEDLAKLVEEKERLKAEAKAKEAEAAEAQKRSAKAAVSSLLSSKLFANKEGEKES 600

BLAST of Cucsa.032120 vs. NCBI nr
Match: gi|596020758|ref|XP_007218947.1| (hypothetical protein PRUPE_ppa002582mg [Prunus persica])

HSP 1 Score: 908.7 bits (2347), Expect = 5.6e-261
Identity = 457/599 (76.29%), Postives = 510/599 (85.14%), Query Frame = 1

Query: 1   MALSKPAFFTHLKTLT---------------------------ADAERRRRKRRLRIEPP 60
           MALSKP F THL+TL                            A AERRRRKRRLRIEPP
Sbjct: 1   MALSKPTFLTHLRTLAKPPNCHHPTTPPSFISLRFLSFATPEEAAAERRRRKRRLRIEPP 60

Query: 61  LSSSSAARPLTQPPRSQTPQ-NPNAPKIPEHISALSGNRLNLHNRILTLIRENDLEEAAL 120
           LSS    +   Q  +S  PQ NPNAPK+PE +SALSGNRLNLHNRILTL+R+NDLEEAAL
Sbjct: 61  LSSLHRNQQQQQQQQSPKPQQNPNAPKLPEPVSALSGNRLNLHNRILTLVRQNDLEEAAL 120

Query: 121 FTRHSIYSNCRPTIFTVNAVLNAQLRQSKYADLLSLHRFITQAGVVPNIITHNLIFQTYL 180
           +TRHSIYSNCRPTIFTVN+VL AQLRQSKY+DLLSLHRFITQAGV PNIITHNLIFQTYL
Sbjct: 121 YTRHSIYSNCRPTIFTVNSVLTAQLRQSKYSDLLSLHRFITQAGVAPNIITHNLIFQTYL 180

Query: 181 DCRKPDTAMEHYKQLINDAPFNPSPTTYRILIKGLVDNNKLERAMELKDEMIEKGFAPDP 240
           DCRKPDTAME+YKQLINDAPFNPSPTTYRILIKGLVDNNKL+RAMELK+E+  KGFAPDP
Sbjct: 181 DCRKPDTAMENYKQLINDAPFNPSPTTYRILIKGLVDNNKLDRAMELKEEIDAKGFAPDP 240

Query: 241 LIYHYLMGGCVRSLDPDGVFKLFEELKEKLGATVEDGVVYGNLMKGYFMKEMEEEAMKCY 300
           ++YHYLM GCV++ D DGVF+L+EELKEKLG  VEDG+VYGNLMKGYFM+ ME+EAM+CY
Sbjct: 241 VVYHYLMVGCVKNSDSDGVFRLYEELKEKLGGVVEDGIVYGNLMKGYFMRGMEKEAMECY 300

Query: 301 EETVGDNSVVKMSAIAYNSVLDALCRNGKFGEALTLFDRMTKEHRPPRHLAVNLGSFNVM 360
           EE+ G++S VK SA+AYNSVLDAL +NGKF EAL LFDRM  EH PPR LAVNLGSFNVM
Sbjct: 301 EESFGESSKVKTSAVAYNSVLDALSKNGKFDEALRLFDRMVAEHNPPRRLAVNLGSFNVM 360

Query: 361 VDGYCIEGRFKEAIEVFEKMGDYRCCPDTLSFNNLIEQLCNNGMLAEAEMLYGTMDDKGV 420
            DGYC++GRFKEAIEVF KMGDYRC PDTLSFNNLIEQLC NGML+EAE LYG M DKGV
Sbjct: 361 ADGYCVQGRFKEAIEVFRKMGDYRCSPDTLSFNNLIEQLCKNGMLSEAEELYGEMSDKGV 420

Query: 421 NPDEFTYGLLMDSCFKKNRADDAAAYFRKMVDSGLRPNIAVYNILVDELVKLGKIDDAKS 480
            PDEFTY LLMD+CF++NRADDAA YFRKMVD+ LRPN+AVYN LVD L+K+GK+D+AKS
Sbjct: 421 YPDEFTYVLLMDTCFEENRADDAAEYFRKMVDAKLRPNLAVYNRLVDGLIKVGKVDEAKS 480

Query: 481 FFDLMVKKLKMDASSYQFIMKALSESGKMDEILNVVDTLLDDDGIEFSEELQEFVRGELR 540
           FFDLMVKKLKMD  SYQFIMK LSE+GK+DE+LNVVDT+LDDDG+EF+EELQEFV+GELR
Sbjct: 481 FFDLMVKKLKMDIPSYQFIMKTLSEAGKLDEVLNVVDTMLDDDGVEFNEELQEFVKGELR 540

Query: 541 KENREEDLAKLVEEKERLKAEAKAKEAEAAEAQKRSAKAAVSSLLSSKLFANKEGEKES 572
           KE RE+++ KL+EEKER KAEAKAKEAEAAEA KRSA+AAVSSLL SKLF NKE E  S
Sbjct: 541 KEGREDEVGKLMEEKERQKAEAKAKEAEAAEAAKRSARAAVSSLLPSKLFGNKESETGS 599

BLAST of Cucsa.032120 vs. NCBI nr
Match: gi|645258146|ref|XP_008234749.1| (PREDICTED: pentatricopeptide repeat-containing protein At3g49240 [Prunus mume])

HSP 1 Score: 902.1 bits (2330), Expect = 5.2e-259
Identity = 456/601 (75.87%), Postives = 511/601 (85.02%), Query Frame = 1

Query: 1   MALSKPAFFTHLKTLT---------------------------ADAERRRRKRRLRIEPP 60
           MALSKP F THL+TL                            A AERRRRKRRLRIEPP
Sbjct: 1   MALSKPTFLTHLRTLAKPPNCHHPTPPPSFISLRFLSFATPEEAAAERRRRKRRLRIEPP 60

Query: 61  LSSSSAARPLTQPPRSQTP---QNPNAPKIPEHISALSGNRLNLHNRILTLIRENDLEEA 120
           LSS    +   Q  + Q+P   QNPNAPK+PE +SALSGNRLNLHNRILTL+R+NDLEEA
Sbjct: 61  LSSLHRNQQ-QQQQQQQSPKPQQNPNAPKLPEPVSALSGNRLNLHNRILTLVRQNDLEEA 120

Query: 121 ALFTRHSIYSNCRPTIFTVNAVLNAQLRQSKYADLLSLHRFITQAGVVPNIITHNLIFQT 180
           AL+TRHSIYSNCRPTIFTVN+VL AQLRQSKY+DLLSLHRFITQAGV PNIITHNLIFQT
Sbjct: 121 ALYTRHSIYSNCRPTIFTVNSVLTAQLRQSKYSDLLSLHRFITQAGVAPNIITHNLIFQT 180

Query: 181 YLDCRKPDTAMEHYKQLINDAPFNPSPTTYRILIKGLVDNNKLERAMELKDEMIEKGFAP 240
           YLDCRKPDTAME+YKQLINDAPFNPSPTTYRILIKGLVDNNKL+RAMELK+E+  KGFAP
Sbjct: 181 YLDCRKPDTAMENYKQLINDAPFNPSPTTYRILIKGLVDNNKLDRAMELKEEIDVKGFAP 240

Query: 241 DPLIYHYLMGGCVRSLDPDGVFKLFEELKEKLGATVEDGVVYGNLMKGYFMKEMEEEAMK 300
           DP++YHYLM GCV++ D DGVFKL+EELKEKLG  VEDG+VYGNLMKGYFM+ ME+EAM+
Sbjct: 241 DPVVYHYLMVGCVKNSDSDGVFKLYEELKEKLGGVVEDGIVYGNLMKGYFMRGMEKEAME 300

Query: 301 CYEETVGDNSVVKMSAIAYNSVLDALCRNGKFGEALTLFDRMTKEHRPPRHLAVNLGSFN 360
           CYEE++ ++S VKMSA+AYNSVLDAL +NGKF EAL LFDRM  EH PPR LAVNLGSFN
Sbjct: 301 CYEESLRESSKVKMSAVAYNSVLDALSKNGKFDEALRLFDRMVAEHNPPRRLAVNLGSFN 360

Query: 361 VMVDGYCIEGRFKEAIEVFEKMGDYRCCPDTLSFNNLIEQLCNNGMLAEAEMLYGTMDDK 420
           VM DGYC EGRFKEAIEVF KMGDYRC PDTLSFNNLIEQLC NGML+EAE LYG M DK
Sbjct: 361 VMADGYCAEGRFKEAIEVFRKMGDYRCSPDTLSFNNLIEQLCKNGMLSEAEELYGEMSDK 420

Query: 421 GVNPDEFTYGLLMDSCFKKNRADDAAAYFRKMVDSGLRPNIAVYNILVDELVKLGKIDDA 480
           GVN DE+TY LLMD+CF++NRADDAA YFRKMVD+ LRPN+AVYN LVD L+K+GK+D+A
Sbjct: 421 GVNADEYTYVLLMDTCFEENRADDAAEYFRKMVDAKLRPNLAVYNRLVDGLIKVGKVDEA 480

Query: 481 KSFFDLMVKKLKMDASSYQFIMKALSESGKMDEILNVVDTLLDDDGIEFSEELQEFVRGE 540
           KSFFDLMVKKLKMD  SYQFIMK LSE+GK+DE+LNVV+T+LDDDG+EF+EELQEFV+GE
Sbjct: 481 KSFFDLMVKKLKMDIPSYQFIMKTLSEAGKLDEVLNVVNTMLDDDGVEFNEELQEFVKGE 540

Query: 541 LRKENREEDLAKLVEEKERLKAEAKAKEAEAAEAQKRSAKAAVSSLLSSKLFANKEGEKE 572
           +RKE RE+++ KL+EEKER KAEAKAKEAEAAEA KRSA+AAVSSLL SKLF NKE E  
Sbjct: 541 MRKEGREDEVGKLMEEKERQKAEAKAKEAEAAEAAKRSARAAVSSLLPSKLFGNKESETG 600

BLAST of Cucsa.032120 vs. NCBI nr
Match: gi|694447816|ref|XP_009349953.1| (PREDICTED: pentatricopeptide repeat-containing protein At3g49240-like [Pyrus x bretschneideri])

HSP 1 Score: 899.4 bits (2323), Expect = 3.4e-258
Identity = 458/637 (71.90%), Postives = 512/637 (80.38%), Query Frame = 1

Query: 1   MALSKPAFFTHLKTLT--------------------------ADAERRRRKRRLRIEPPL 60
           MALSKP F THLKTL                           A AERR+RKRRLR+EPPL
Sbjct: 1   MALSKPTFLTHLKTLAKPPIRRRLAPPSFISLRFLSFATPEEAAAERRQRKRRLRLEPPL 60

Query: 61  SSSSAARPLTQPPRSQTPQNPNAPKIPEHISALSGNRLNLHNRILTLIRENDLEEAALFT 120
           SS    +   Q    +   NPNAPK+PE +SALSGNRLNLHNRILTL+R+NDLEEAAL+T
Sbjct: 61  SSLHRNQQQQQQQSPKPQINPNAPKLPETVSALSGNRLNLHNRILTLVRQNDLEEAALYT 120

Query: 121 RHSIYSNCRPTIFTVNAVLNAQLRQSKYADLLSLHRFITQAGVVPNIITHNLIFQTYLDC 180
           RHSIYSNCRPTIFTVNAVL AQLRQSKY+DLL+LHRFITQAGV PNIITHNLIFQTY+DC
Sbjct: 121 RHSIYSNCRPTIFTVNAVLTAQLRQSKYSDLLTLHRFITQAGVAPNIITHNLIFQTYMDC 180

Query: 181 RKPDTAMEHYKQLINDAPFNPSPTTYRILIKGLVDNNKLERAMELKDEMIEKGFAPDPLI 240
           RKPDTAMEHYKQLINDAPFNPSPTTYRILIKGLVDNNKL+RAMELK+E+  KGFAPDP++
Sbjct: 181 RKPDTAMEHYKQLINDAPFNPSPTTYRILIKGLVDNNKLDRAMELKEEIDVKGFAPDPVV 240

Query: 241 YHYLMGGCVRSLDPDGVFKLFEELKEKLGATVEDGVVYGNLMKGYFMKEMEEEAMKCYEE 300
           YHYLM GCV++ D DGVFKL+EELKEKLG  VEDGVVYGNLMKG+FM+EME+EAM CYEE
Sbjct: 241 YHYLMQGCVKNSDSDGVFKLYEELKEKLGGAVEDGVVYGNLMKGFFMREMEKEAMDCYEE 300

Query: 301 TVGDNSVVKMSAIAYNSVLDALCRNGKFGEALTLFDRMTKEHRPPRHLAVNLGSFNVMVD 360
            VG++S VKMSA+AYNSVLDAL +NGKF EAL LFDRM +EH PPR LAVNLGSFNVM +
Sbjct: 301 AVGESSKVKMSAVAYNSVLDALSKNGKFDEALRLFDRMIQEHNPPRRLAVNLGSFNVMAN 360

Query: 361 GYCIEGRFKEAIEVFEKMGDYRCCPDTLSFNNLIEQLCNNGMLAEAEMLYGTMDDKGVNP 420
           GYC EGRFK+AIEVF KMGDYRC PD LSFNNLIEQLC NGML+EAE LYG M DKGVNP
Sbjct: 361 GYCAEGRFKDAIEVFRKMGDYRCSPDALSFNNLIEQLCKNGMLSEAEELYGEMPDKGVNP 420

Query: 421 DEFTYGLLMDSCFKKNRADDAAAYFRKMVDSGLRPNIAVYNILVDELVKLGKIDDAKSFF 480
           DEFTY LLMD+CF++NR DDAA YFRKMVD+ LRPN+AVYN L D LVK+GKID+AKSFF
Sbjct: 421 DEFTYVLLMDTCFEENRPDDAAEYFRKMVDAKLRPNLAVYNKLADGLVKVGKIDEAKSFF 480

Query: 481 DLMVKKLKMDASSYQFIMKALSESGKMDEILNVVDTLLDDDGIEFSEELQEFVRGELRKE 540
           DLMVKKLKMD  SYQFIMK LS++GK+DE+LNVVDT+LDDDG+EF EE+QEFV+G LRKE
Sbjct: 481 DLMVKKLKMDIPSYQFIMKTLSDAGKLDEVLNVVDTMLDDDGVEFDEEVQEFVKGLLRKE 540

Query: 541 NREEDLAKLVEEKERLKAEAKAKEAEAAEAQKRSAKAAVSSLLSSKLFANKE---GEKES 600
            RE+++ KL+EEKER KAEAKAKE EAAEA KRSA+AAVSSLL SKLF NKE   G  E+
Sbjct: 541 GREDEVGKLMEEKERQKAEAKAKELEAAEAAKRSARAAVSSLLPSKLFGNKEPEAGSTEA 600

Query: 601 VPCRLEPCRAEKPQSEGRNIDQNNEKPAKCEKRKPQD 609
                E   A +    G+      E     E     D
Sbjct: 601 TENASEAASASEAAETGQEERSGEEGQGSAEIESKSD 637

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
PP273_ARATH6.0e-21562.79Pentatricopeptide repeat-containing protein At3g49240 OS=Arabidopsis thaliana GN... [more]
PPR29_ARATH3.7e-7935.07Pentatricopeptide repeat-containing protein At1g10270 OS=Arabidopsis thaliana GN... [more]
PPR28_ARATH2.3e-4126.21Pentatricopeptide repeat-containing protein At1g09900 OS=Arabidopsis thaliana GN... [more]
PPR36_ARATH2.2e-3926.01Pentatricopeptide repeat-containing protein At1g12300, mitochondrial OS=Arabidop... [more]
PPR97_ARATH1.2e-3725.18Pentatricopeptide repeat-containing protein At1g63070, mitochondrial OS=Arabidop... [more]
Match NameE-valueIdentityDescription
A0A0A0L7B2_CUCSA0.0e+0093.78Uncharacterized protein OS=Cucumis sativus GN=Csa_3G239860 PE=4 SV=1[more]
E5GB98_CUCME7.2e-30890.18Pentatricopeptide repeat-containing protein OS=Cucumis melo subsp. melo PE=4 SV=... [more]
M5X3R7_PRUPE3.9e-26176.29Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa002582mg PE=4 SV=1[more]
W9RP26_9ROSA5.1e-25370.15Uncharacterized protein OS=Morus notabilis GN=L484_006813 PE=4 SV=1[more]
A0A061G122_THECC2.8e-24370.42Pentatricopeptide repeat-containing protein, putative OS=Theobroma cacao GN=TCM_... [more]
Match NameE-valueIdentityDescription
AT3G49240.13.4e-21662.79 Pentatricopeptide repeat (PPR) superfamily protein[more]
AT1G10270.12.1e-8035.07 glutamine-rich protein 23[more]
AT1G09900.11.3e-4226.21 Pentatricopeptide repeat (PPR-like) superfamily protein[more]
AT1G12300.11.2e-4026.01 Tetratricopeptide repeat (TPR)-like superfamily protein[more]
AT1G63070.16.7e-3925.18 pentatricopeptide (PPR) repeat-containing protein[more]
Match NameE-valueIdentityDescription
gi|449456969|ref|XP_004146221.1|0.0e+0093.78PREDICTED: pentatricopeptide repeat-containing protein At3g49240 [Cucumis sativu... [more]
gi|659133624|ref|XP_008466825.1|1.0e-30790.18PREDICTED: pentatricopeptide repeat-containing protein At3g49240 [Cucumis melo][more]
gi|596020758|ref|XP_007218947.1|5.6e-26176.29hypothetical protein PRUPE_ppa002582mg [Prunus persica][more]
gi|645258146|ref|XP_008234749.1|5.2e-25975.87PREDICTED: pentatricopeptide repeat-containing protein At3g49240 [Prunus mume][more]
gi|694447816|ref|XP_009349953.1|3.4e-25871.90PREDICTED: pentatricopeptide repeat-containing protein At3g49240-like [Pyrus x b... [more]
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR002885Pentatricopeptide_repeat
IPR011990TPR-like_helical_dom_sf
Vocabulary: Molecular Function
TermDefinition
GO:0005515protein binding
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009793 embryo development ending in seed dormancy
biological_process GO:0008150 biological_process
cellular_component GO:0009507 chloroplast
cellular_component GO:0005739 mitochondrion
cellular_component GO:0005575 cellular_component
molecular_function GO:0005524 ATP binding
molecular_function GO:0005515 protein binding

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cucsa.032120.1Cucsa.032120.1mRNA


Analysis Name: InterPro Annotations of cucumber (Gy14)
Date Performed: 2017-01-17
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002885Pentatricopeptide repeatPFAMPF01535PPRcoord: 250..274
score: 0.22coord: 288..315
score: 1.6E-7coord: 433..460
score: 5.
IPR002885Pentatricopeptide repeatPFAMPF12854PPR_1coord: 324..352
score: 8.
IPR002885Pentatricopeptide repeatPFAMPF13041PPR_2coord: 178..223
score: 5.9E-8coord: 359..407
score: 1.8
IPR002885Pentatricopeptide repeatPFAMPF13812PPR_3coord: 101..151
score: 0.
IPR002885Pentatricopeptide repeatTIGRFAMsTIGR00756TIGR00756coord: 433..460
score: 3.7E-5coord: 363..396
score: 1.6E-5coord: 288..315
score: 6.9E-8coord: 328..361
score: 6.0E-9coord: 397..431
score: 7.1E-7coord: 179..211
score: 2.
IPR002885Pentatricopeptide repeatPROFILEPS51375PPRcoord: 285..319
score: 12.288coord: 176..210
score: 11.762coord: 325..359
score: 12.09coord: 140..170
score: 6.61coord: 360..394
score: 11.224coord: 464..499
score: 7.563coord: 248..282
score: 6.982coord: 105..139
score: 7.355coord: 430..460
score: 9.504coord: 395..429
score: 11.827coord: 211..241
score: 7
IPR011990Tetratricopeptide-like helical domainGENE3DG3DSA:1.25.40.10coord: 75..260
score: 3.6E-5coord: 261..489
score: 2.0
IPR011990Tetratricopeptide-like helical domainunknownSSF48452TPR-likecoord: 146..207
score: 4.74E-8coord: 246..462
score: 4.7
NoneNo IPR availableunknownCoilCoilcoord: 513..552
scor
NoneNo IPR availablePANTHERPTHR24015FAMILY NOT NAMEDcoord: 17..505
score: 1.0E
NoneNo IPR availablePANTHERPTHR24015:SF237SUBFAMILY NOT NAMEDcoord: 17..505
score: 1.0E

The following gene(s) are paralogous to this gene:

None