CsaV3_1G015110 (gene) Cucumber (Chinese Long) v3 | Cucurbit Genomics Database (CuGenDB)

Overview


Name	CsaV3_1G015110
Type	gene
Organism	Cucumis sativus (Cucumber (Chinese Long) v3)
Description	Nitrilase
Location	chr1 : 10725091 .. 10730232 (-)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonpolypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

CACAAATTTCCACCTCCACTCGAAAACTTAGTTTCTAAGTTTTCTCTCCTCATCCACTTGCATTCGCAAATCTCGCCATTCCGTTTGGACGATACTATCATCATCGTTTGTATTAATCAAGCAACTTGACCATCCTGAATAATGAAGTGAATATATATATATATCCTTGTTGCTTCTTCTAGCAACTTCCACAAATTTCATCTATCTACAACGAACGGAGATAAATAATAAAAAGTGTAAAACTCGAGTCCATACATTGCGTTGCCATTGATAGCCCTGTTGCTCGCCGGCTGAAGCTCAAGCTGAGAGAGAGAGAAAAAAAAAAGGGAGATTCAAGCAGCAACCATGGCGCTTGTGCCAACCACGGCCCCTACCGACTCCCAACTGATAGCGGAGGTTGACATGGGCTCCGATTCATCTGTAATGAATGTTCGAGCCACCGTCGTTCAGGCCTCCACCATCTTCTATGACACTCCGGCCACTCTAGGTTCCTTTTCCTTGCTTCTATTTTCAATTTACTCCTTATGTACTACTCTTGCTCTCGCTGTTGCTTTGCTCACTAGCTCTTGGATCGCAATGGTCAGTTCAGAGTTTGGAATGAGTTCCAGATTGTTTTTGCTTGTGTTCTTCGAAGCAAGGTTATTAGTTGTTTTGGCTGTTTTTTTGCTTGGTTTTCTTTAATCTTACAAGTTGCTTTTCTTTCATCAGCTGATTTTGGTTGAATAATTTAATTAGTTTAAACTATTACATCCTCGATTACTTTTCCTGGTTTTAATTGTCTCTCTTGTCTACGATCAATATGAATTTGCTGCTGTGGCCTAACATTGTGCTCCTGATTGTTCTTTTCTCCCTTAATTTTACTCGATTGGAGACATTGAAGAGAAAATGTACACGTGAAATACCACGGCTTAAAATGATTTAATACATGTGGGCCTTTTTTATTCTTCCTCTGTAATGGTAAAAAGAAATCTAACTCTGTTTTACATGAACAGATAAGGCTGAGAGGTTGTTGGCTGAAGCTGCTGGATTTGGTTCCCAGCTAGTTGTATTTCCAGAAGCCTTTGTTGGTGGTTATCCTCGTGGATCAAATTTTGGTGTTTCCATTGGGAATAGAACTCCTAAGGGGAAAGAGGAATTTCGTAAATATCATGCCTCAGCGATTGATGTACCTGGTTAGTTTTTGTCTCCAGGTGCACTTTGTTTGACTCAATGTGAGTTGCTACTGTAAAGAGAAAAATCTTAGTGTCTTACCATGGATAATTTCAGGTTAAGTAAGTTTTGCTAGGCTCGTTGTATAATTCTCATTCTAGAGGGCTGACTTAATTTCTCCTTGAGTTTACCAAACTTGATTTCTGGTGATATATTAGTATTAAATTCTTTCATCGTAACATACACCTTCTGTTCTTTATCATCTTTCATTCCTAAATGGATGTTACCTCATATTAGGACAGTACAATAAGGAGCTACTGATGTCAGTCTATTCAAAGTGAAATACGAGTTTGTTGCTCATGAAATATGAAATGGGACTATGGTCTGTTTTAATGGCTCTTGCAGTTTGCAGTTAATGAATGACAAACAAGATTGCTTCAACTGCTGGCATTTCTTAGTTCTATTAATTTCAAAATCTTCCTACTCTTGCTTGCATCTAAAACCTAGACATAATATTGTAAAGTTCTGAAAGCTATAGCCAGTTCCTGTCTTAGTTGCTGTTTAGGTTTTTTTTTAAAACTCTATTTTGATAGGCTTCAGTTCATGTATGAGGTTGCTTTACTTCCATTCATCCTAATACAATCTGGTTGACCTTGTGTTTACTTCCATTCATCTAAGTAATATCTTGTTTTCTCATAAAAACCATTTTTATTCATCTATAAGATCATATGCCACATTCACAAAATAAACTGCTTGAAGTGCACCGGCTGTGGTTCTAATATATCAAATGATTGTTATTTTGATGACTATAGACGGTAATACATGCAGGTCCTGAAGTTGATCGATTGGCAGCAATGGCTGGAAAGTATAGGGTACATTTGGTAATGGGGGTGATAGAGAGAGATGGCTACACACTGTACTGTACTGTCCTATTTTTTGATCCTCAAGGTCGTTACTTGGGTAAGCACAGGAAAGTCATGCCAACTGCATTGGAGCGAATAATCTGGGGCTTTGGTGATGGATCAACAATTCCTGTGTTTGAAACTTCAATTGGAAAGATAGGTGCTGCCATTTGTTGGGAAAACAGAATGCCACTCCTAAGAACAGCTATGTATGCAAAAGGTTAGTTATTCATTGCTGGCAATAATGTCATTCCTGACGTAATTGCGTATTTCAAATCTAGTTTTATTCTTTCTGTTTGACTGTATGATGTTTATTAAACTTAAAATAGATAGCATAGCACTACAAAAATGTGATGGGAATGGTATTAGAGGGATATTAAGGGTAAATTAGTAGTAAGTTAAAGAGTTTGTCAGGGAAGTTTGGTTATAAATATAATGAGTAAGGAAGAAAGGGTTTGACAATATTTTGGGAGTAAATTAGGGCTTTGAGTGACCTCAAGATTGGGAGGGTCCAAGTACCTCAAATTACATGGTCTATTTTGTATTTCTCTTATCTTTTATAGTTCAATATATTTGGGTTCTACCAAAAGACTGACGAGAAACTATTACAGACTAAAGAGGCTCTAGTTGTTAAGAAATTGTAAAACGATGGTCAAACTCCAGAGTTGGGGATTATTGTGTCACTGCTCACTATTTACAGACACTGGTCTATCATTTTTAGCTTCTTTTCTCAGAATATACTTGTTTGGTTAATCATATTGTGCACAACTTTTTTGTTTTATGTTTCTCTTTCAACTACATCAAGTTAAAAAGTACTTTACACCGAAACCAAATGGAAAAATCATATAGAAGAAGTTTTTCATATGCTTAAAGTTCTACTGCTATGTTAAGGATAGAAGTTGGTATTTCTTCAAACTTGCCTAACTTCCGTCTAAACAGTCCTTTTCTTTTGCTTAACCGAATTTGCTCACTAGTAATTGACTTATCATGTTTGTAGGCATTGAAATTTATTGTGCACCTACTGCTGATTCTCGGGATACATGGCAAGCATCAATGACCCACATTGCTTTGGAGGGTGGATGTTTTGTTTTGTCAGCCAACCAGTTCTGCCGAAGAAAAGACTACCCACCTCCACCAGAATATGTATTTTCTGGCACAGAAGAGGAACTGACCCCGGATTCTGTTGTTTGTGCTGGAGGTAGTGCGATCATATCACCTTCAGGGACAATCCTAGCTGGACCCAATTACGATGGAGAGGCACTTATTTCAGCAGATCTGGGTATGTCATCTCATCCTTCTGCTTATGGTCATCAGAGCTTTAGTATGTACTCCAACATTATCATTTTAAGTGGTTGATATAAGAAGGTTTTATCTTGAGGAACCTACTATTTTATTTGAGTCTCTTTCTTCTTCAAGTGAATACTAGGATTCTCTCTCCTGGATGATCTGATGCTCCTGACAGCCAACGATTCTGAAAACCTTTATTCCAAGCTCTTATTCTAAGCTTAGCCATTTTATTGTCAATATAAATGAACGGCCACTAGGATTTGGTTTCCAGTAAAATGGTTAAGGATCCATCAACCATTAAAGAGAGGAAGGGCCTCTCTTGCTGTCTAAAAAGTTCCTTGGAGAATGTGGGGATAAACCGAAGTGGTTTTTTTTAACCTTATGTGCCTTGAGTTATGAAACTTTTTCATCTAGATCTTTGTGAGCAAAGGTCGAACTAGGAATTCCTAAGCTAAGCCTCTTACCAAGGTAGAAGGGTTTTTGTGAAGGTTCTACTTGATAATATAAATAGAAAGCACAAAATTACATGGACAAGGCAATAACAACAATCTAACCACGAAAGGCCGAATAAAGGCCCAATCAAATCCCTTGAGGGAAAATTTTGATTTACCCCTTCTAACATGTCTGTGCACTATGTGGTCCTCACAGTTCGAATATTGAAGAATGTTAGATACCAAAGAGAGCTCCTAATATTTGACAGGCCAGGAAGACAGGAAGCAATAAAGCAAGAAAGAGTTATTGGCTTATCGCTATCGGATTTTTAATCTCCTATGACAGTCCCTCTTAGAAAAGCACAAATACAAAAAAGAACCCCTCCTAAGATCGGTCACTAATGCCAGCTACTTAACCATAAGAGATTGGTCTTCAAGCCAAACATATTCTTAAATGAATAAAGGATTGAAGACGGATACTCAAATCTCCCAATCATATGCCTGCAAATAAAGGTTAGAGAGATAAACCTGGTTCCAACTGATTTTTGGCACGGTTCAGTATGAATGATTATTGATCCATCCACACCAACTCTTAACAAATTAAACTTTTTGGAGCATATGTTTTATTGACTATTTACCCCTCCCTTCATTGATTGTTATGGAAAGCTTTTTTCTACAACTAGCATTTTTAGATGAAAAATAATTTCGATGAATATCTGAAACAGATCTTGGAGAAATAGCACGGGCAAAATTTGATTTCGATGTGGTTGGTCATTATGCAAGGCCTGAAGTTCTTAGCTTGGTTGTGAGGGATCATCCTACAACCCCGGTTACTTTCACATCTACATCTACAAAAGTCGAAGATTCTTGTAAAAAGTAGTACGTATTCTTCGTTCACACTTCTATTTAGTTCCATTAATTGTTTTAGCCAACATGCGTCCATGTTTTACTGTTTCCTCCGCGTTCAGTGTTGTATTATATTCATACTAAGAATTCAAAACTGTGAATATTTCTGTTAATTTTTATCAGAATTATATTTGCAGCAATTTGTCACGGTGGGTTTCTGTTCACATCATTTCCCCTCTATTTCTCTAACCTAGCTTGCTTATGATTCTGGATTTAGCTGTATCACTGATGCTAGTGTAAGAAATGTGGCCAATTAAGTTTTAGTATATTTGTGTTTGATACTTTGACAAGAGCATATAGGTTATTTTATTTTATTTGATACCTTCCTCAAAAAATCATATCATTCCTCTTCCTCTTCCTCTAATATCTCAATCCATCCAACACATCGTATTAAGCCACAAGAAACGGTCAAGTGACTTGTTTTATTTTATTTTGTAACCTTATTTATGTTAAAGTTCTGAAAAAAC

mRNA sequence

ATGGCGCTTGTGCCAACCACGGCCCCTACCGACTCCCAACTGATAGCGGAGGTTGACATGGGCTCCGATTCATCTGTAATGAATGTTCGAGCCACCGTCGTTCAGGCCTCCACCATCTTCTATGACACTCCGGCCACTCTAGATAAGGCTGAGAGGTTGTTGGCTGAAGCTGCTGGATTTGGTTCCCAGCTAGTTGTATTTCCAGAAGCCTTTGTTGGTGGTTATCCTCGTGGATCAAATTTTGGTGTTTCCATTGGGAATAGAACTCCTAAGGGGAAAGAGGAATTTCGTAAATATCATGCCTCAGCGATTGATGTACCTGGTCCTGAAGTTGATCGATTGGCAGCAATGGCTGGAAAGTATAGGGTACATTTGGTAATGGGGGTGATAGAGAGAGATGGCTACACACTGTACTGTACTGTCCTATTTTTTGATCCTCAAGGTCGTTACTTGGGTAAGCACAGGAAAGTCATGCCAACTGCATTGGAGCGAATAATCTGGGGCTTTGGTGATGGATCAACAATTCCTGTGTTTGAAACTTCAATTGGAAAGATAGGTGCTGCCATTTGTTGGGAAAACAGAATGCCACTCCTAAGAACAGCTATGTATGCAAAAGGCATTGAAATTTATTGTGCACCTACTGCTGATTCTCGGGATACATGGCAAGCATCAATGACCCACATTGCTTTGGAGGGTGGATGTTTTGTTTTGTCAGCCAACCAGTTCTGCCGAAGAAAAGACTACCCACCTCCACCAGAATATGTATTTTCTGGCACAGAAGAGGAACTGACCCCGGATTCTGTTGTTTGTGCTGGAGGTAGTGCGATCATATCACCTTCAGGGACAATCCTAGCTGGACCCAATTACGATGGAGAGGCACTTATTTCAGCAGATCTGGATCTTGGAGAAATAGCACGGGCAAAATTTGATTTCGATGTGGTTGGTCATTATGCAAGGCCTGAAGTTCTTAGCTTGGTTGTGAGGGATCATCCTACAACCCCGGTTACTTTCACATCTACATCTACAAAAGTCGAAGATTCTTGTAAAAAGTAG

Coding sequence (CDS)

ATGGCGCTTGTGCCAACCACGGCCCCTACCGACTCCCAACTGATAGCGGAGGTTGACATGGGCTCCGATTCATCTGTAATGAATGTTCGAGCCACCGTCGTTCAGGCCTCCACCATCTTCTATGACACTCCGGCCACTCTAGATAAGGCTGAGAGGTTGTTGGCTGAAGCTGCTGGATTTGGTTCCCAGCTAGTTGTATTTCCAGAAGCCTTTGTTGGTGGTTATCCTCGTGGATCAAATTTTGGTGTTTCCATTGGGAATAGAACTCCTAAGGGGAAAGAGGAATTTCGTAAATATCATGCCTCAGCGATTGATGTACCTGGTCCTGAAGTTGATCGATTGGCAGCAATGGCTGGAAAGTATAGGGTACATTTGGTAATGGGGGTGATAGAGAGAGATGGCTACACACTGTACTGTACTGTCCTATTTTTTGATCCTCAAGGTCGTTACTTGGGTAAGCACAGGAAAGTCATGCCAACTGCATTGGAGCGAATAATCTGGGGCTTTGGTGATGGATCAACAATTCCTGTGTTTGAAACTTCAATTGGAAAGATAGGTGCTGCCATTTGTTGGGAAAACAGAATGCCACTCCTAAGAACAGCTATGTATGCAAAAGGCATTGAAATTTATTGTGCACCTACTGCTGATTCTCGGGATACATGGCAAGCATCAATGACCCACATTGCTTTGGAGGGTGGATGTTTTGTTTTGTCAGCCAACCAGTTCTGCCGAAGAAAAGACTACCCACCTCCACCAGAATATGTATTTTCTGGCACAGAAGAGGAACTGACCCCGGATTCTGTTGTTTGTGCTGGAGGTAGTGCGATCATATCACCTTCAGGGACAATCCTAGCTGGACCCAATTACGATGGAGAGGCACTTATTTCAGCAGATCTGGATCTTGGAGAAATAGCACGGGCAAAATTTGATTTCGATGTGGTTGGTCATTATGCAAGGCCTGAAGTTCTTAGCTTGGTTGTGAGGGATCATCCTACAACCCCGGTTACTTTCACATCTACATCTACAAAAGTCGAAGATTCTTGTAAAAAGTAG

Protein sequence

MALVPTTAPTDSQLIAEVDMGSDSSVMNVRATVVQASTIFYDTPATLDKAERLLAEAAGFGSQLVVFPEAFVGGYPRGSNFGVSIGNRTPKGKEEFRKYHASAIDVPGPEVDRLAAMAGKYRVHLVMGVIERDGYTLYCTVLFFDPQGRYLGKHRKVMPTALERIIWGFGDGSTIPVFETSIGKIGAAICWENRMPLLRTAMYAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSANQFCRRKDYPPPPEYVFSGTEEELTPDSVVCAGGSAIISPSGTILAGPNYDGEALISADLDLGEIARAKFDFDVVGHYARPEVLSLVVRDHPTTPVTFTSTSTKVEDSCKK

Homology

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BLAST of CsaV3_1G015110 vs. NCBI nr
Match: XP_004139500.1 (PREDICTED: bifunctional nitrilase/nitrile hydratase NIT4A [Cucumis sativus] >KGN64959.1 hypothetical protein Csa_1G168360 [Cucumis sativus])

HSP 1 Score: 713.8 bits (1841), Expect = 2.9e-202
Identity = 350/350 (100.00%), Postives = 350/350 (100.00%), Query Frame = 0


Query: 1   MALVPTTAPTDSQLIAEVDMGSDSSVMNVRATVVQASTIFYDTPATLDKAERLLAEAAGF 60
           MALVPTTAPTDSQLIAEVDMGSDSSVMNVRATVVQASTIFYDTPATLDKAERLLAEAAGF
Sbjct: 1   MALVPTTAPTDSQLIAEVDMGSDSSVMNVRATVVQASTIFYDTPATLDKAERLLAEAAGF 60

Query: 61  GSQLVVFPEAFVGGYPRGSNFGVSIGNRTPKGKEEFRKYHASAIDVPGPEVDRLAAMAGK 120
           GSQLVVFPEAFVGGYPRGSNFGVSIGNRTPKGKEEFRKYHASAIDVPGPEVDRLAAMAGK
Sbjct: 61  GSQLVVFPEAFVGGYPRGSNFGVSIGNRTPKGKEEFRKYHASAIDVPGPEVDRLAAMAGK 120

Query: 121 YRVHLVMGVIERDGYTLYCTVLFFDPQGRYLGKHRKVMPTALERIIWGFGDGSTIPVFET 180
           YRVHLVMGVIERDGYTLYCTVLFFDPQGRYLGKHRKVMPTALERIIWGFGDGSTIPVFET
Sbjct: 121 YRVHLVMGVIERDGYTLYCTVLFFDPQGRYLGKHRKVMPTALERIIWGFGDGSTIPVFET 180

Query: 181 SIGKIGAAICWENRMPLLRTAMYAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSAN 240
           SIGKIGAAICWENRMPLLRTAMYAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSAN
Sbjct: 181 SIGKIGAAICWENRMPLLRTAMYAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSAN 240

Query: 241 QFCRRKDYPPPPEYVFSGTEEELTPDSVVCAGGSAIISPSGTILAGPNYDGEALISADLD 300
           QFCRRKDYPPPPEYVFSGTEEELTPDSVVCAGGSAIISPSGTILAGPNYDGEALISADLD
Sbjct: 241 QFCRRKDYPPPPEYVFSGTEEELTPDSVVCAGGSAIISPSGTILAGPNYDGEALISADLD 300

Query: 301 LGEIARAKFDFDVVGHYARPEVLSLVVRDHPTTPVTFTSTSTKVEDSCKK 351
           LGEIARAKFDFDVVGHYARPEVLSLVVRDHPTTPVTFTSTSTKVEDSCKK
Sbjct: 301 LGEIARAKFDFDVVGHYARPEVLSLVVRDHPTTPVTFTSTSTKVEDSCKK 350

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BLAST of CsaV3_1G015110 vs. NCBI nr
Match: XP_008463648.1 (PREDICTED: bifunctional nitrilase/nitrile hydratase NIT4A [Cucumis melo])

HSP 1 Score: 701.4 bits (1809), Expect = 1.5e-198
Identity = 343/350 (98.00%), Postives = 345/350 (98.57%), Query Frame = 0


Query: 1   MALVPTTAPTDSQLIAEVDMGSDSSVMNVRATVVQASTIFYDTPATLDKAERLLAEAAGF 60
           MALVPTTAPTD QLIAEVDMG+DSSVM VRATVVQASTIFYDTPATLDKAERLLAEAAGF
Sbjct: 1   MALVPTTAPTDPQLIAEVDMGTDSSVMTVRATVVQASTIFYDTPATLDKAERLLAEAAGF 60

Query: 61  GSQLVVFPEAFVGGYPRGSNFGVSIGNRTPKGKEEFRKYHASAIDVPGPEVDRLAAMAGK 120
           GSQLVVFPEAFVGGYPRGSNFGVSIGNRTPKGKEEFRKYHA+AIDVPGPEVDRLAAMAGK
Sbjct: 61  GSQLVVFPEAFVGGYPRGSNFGVSIGNRTPKGKEEFRKYHAAAIDVPGPEVDRLAAMAGK 120

Query: 121 YRVHLVMGVIERDGYTLYCTVLFFDPQGRYLGKHRKVMPTALERIIWGFGDGSTIPVFET 180
           YRVHLVMGVIERDGYTLYCTVLFFDPQGRYLGKHRKVMPTALERIIWGFGDGSTIPVFET
Sbjct: 121 YRVHLVMGVIERDGYTLYCTVLFFDPQGRYLGKHRKVMPTALERIIWGFGDGSTIPVFET 180

Query: 181 SIGKIGAAICWENRMPLLRTAMYAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSAN 240
           SIGKIGAAICWENRMPLLRTAMYAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSAN
Sbjct: 181 SIGKIGAAICWENRMPLLRTAMYAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSAN 240

Query: 241 QFCRRKDYPPPPEYVFSGTEEELTPDSVVCAGGSAIISPSGTILAGPNYDGEALISADLD 300
           QFCRRKDYPPPPEYVFSGTEEELTPDSVVCAGGSAIISPSGTILAGPNYDGEALISADLD
Sbjct: 241 QFCRRKDYPPPPEYVFSGTEEELTPDSVVCAGGSAIISPSGTILAGPNYDGEALISADLD 300

Query: 301 LGEIARAKFDFDVVGHYARPEVLSLVVRDHPTTPVTFTSTSTKVEDSCKK 351
           LGEIARAKFDFDVVGHYARPEVLSLVVRDHP  PVTFTSTSTK EDSCKK
Sbjct: 301 LGEIARAKFDFDVVGHYARPEVLSLVVRDHPAIPVTFTSTSTKAEDSCKK 350

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BLAST of CsaV3_1G015110 vs. NCBI nr
Match: XP_023520715.1 (bifunctional nitrilase/nitrile hydratase NIT4A [Cucurbita pepo subsp. pepo])

HSP 1 Score: 692.2 bits (1785), Expect = 9.2e-196
Identity = 338/350 (96.57%), Postives = 343/350 (98.00%), Query Frame = 0


Query: 1   MALVPTTAPTDSQLIAEVDMGSDSSVMNVRATVVQASTIFYDTPATLDKAERLLAEAAGF 60
           MALVPTTA TD+QLIAEVDMGSD SV  VRATVVQASTIFYDTPATLDKAERLLAEAAGF
Sbjct: 1   MALVPTTAATDTQLIAEVDMGSDVSVPTVRATVVQASTIFYDTPATLDKAERLLAEAAGF 60

Query: 61  GSQLVVFPEAFVGGYPRGSNFGVSIGNRTPKGKEEFRKYHASAIDVPGPEVDRLAAMAGK 120
           GSQLVVFPEAFVGGYPRGSNFGVSIGNRTPKGKEEFRKYHA+AIDVPGPEVDRLAAMAGK
Sbjct: 61  GSQLVVFPEAFVGGYPRGSNFGVSIGNRTPKGKEEFRKYHAAAIDVPGPEVDRLAAMAGK 120

Query: 121 YRVHLVMGVIERDGYTLYCTVLFFDPQGRYLGKHRKVMPTALERIIWGFGDGSTIPVFET 180
           YRVHLVMGVIERDGYTLYCTVLFFDPQGRYLGKHRKVMPTALERIIWGFGDGSTIPVFET
Sbjct: 121 YRVHLVMGVIERDGYTLYCTVLFFDPQGRYLGKHRKVMPTALERIIWGFGDGSTIPVFET 180

Query: 181 SIGKIGAAICWENRMPLLRTAMYAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSAN 240
           SIGKIGAAICWENRMPLLRTAMYAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSAN
Sbjct: 181 SIGKIGAAICWENRMPLLRTAMYAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSAN 240

Query: 241 QFCRRKDYPPPPEYVFSGTEEELTPDSVVCAGGSAIISPSGTILAGPNYDGEALISADLD 300
           QFCRRKDYPPPP+YVFSGTEEEL+PDSVVCAGGS IISPSGTILAGPNYDGEALISADLD
Sbjct: 241 QFCRRKDYPPPPDYVFSGTEEELSPDSVVCAGGSVIISPSGTILAGPNYDGEALISADLD 300

Query: 301 LGEIARAKFDFDVVGHYARPEVLSLVVRDHPTTPVTFTSTSTKVEDSCKK 351
           LG+IARAKFDFDVVGHYARPEVLSLVVRDHPTTPVTFTS STK EDSCKK
Sbjct: 301 LGDIARAKFDFDVVGHYARPEVLSLVVRDHPTTPVTFTSASTKTEDSCKK 350

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BLAST of CsaV3_1G015110 vs. NCBI nr
Match: XP_022973146.1 (bifunctional nitrilase/nitrile hydratase NIT4A [Cucurbita maxima])

HSP 1 Score: 686.0 bits (1769), Expect = 6.6e-194
Identity = 335/350 (95.71%), Postives = 340/350 (97.14%), Query Frame = 0


Query: 1   MALVPTTAPTDSQLIAEVDMGSDSSVMNVRATVVQASTIFYDTPATLDKAERLLAEAAGF 60
           MALVPTTA  D+QLIAEVDMGSD SV  VRATVVQASTIFYDTPATLDKAERLLAEAAGF
Sbjct: 1   MALVPTTAANDTQLIAEVDMGSDVSVPTVRATVVQASTIFYDTPATLDKAERLLAEAAGF 60

Query: 61  GSQLVVFPEAFVGGYPRGSNFGVSIGNRTPKGKEEFRKYHASAIDVPGPEVDRLAAMAGK 120
           GSQLVVFPEAFVGGYPRGSNFGVSIGNRTPKGKEEFRKYHA+AIDVPGPEVDRLAAMAGK
Sbjct: 61  GSQLVVFPEAFVGGYPRGSNFGVSIGNRTPKGKEEFRKYHAAAIDVPGPEVDRLAAMAGK 120

Query: 121 YRVHLVMGVIERDGYTLYCTVLFFDPQGRYLGKHRKVMPTALERIIWGFGDGSTIPVFET 180
           YRVHLVMGVIERDGYTLYCTVLFFDPQGRYLGKHRKVMPTALERIIWGFGDGSTIPVFET
Sbjct: 121 YRVHLVMGVIERDGYTLYCTVLFFDPQGRYLGKHRKVMPTALERIIWGFGDGSTIPVFET 180

Query: 181 SIGKIGAAICWENRMPLLRTAMYAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSAN 240
           SIGKIGAAICWENRMPLLRTAMYAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSAN
Sbjct: 181 SIGKIGAAICWENRMPLLRTAMYAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSAN 240

Query: 241 QFCRRKDYPPPPEYVFSGTEEELTPDSVVCAGGSAIISPSGTILAGPNYDGEALISADLD 300
           QFCRRKDYPPPP+YVFSGTEEEL+PDSVVCAGGS IISPSGTILAGPNYDGEALISADLD
Sbjct: 241 QFCRRKDYPPPPDYVFSGTEEELSPDSVVCAGGSVIISPSGTILAGPNYDGEALISADLD 300

Query: 301 LGEIARAKFDFDVVGHYARPEVLSLVVRDHPTTPVTFTSTSTKVEDSCKK 351
           LG+IARAKFDFDVVGHYARPEVLSLVVRDHPTTPVTFTS S K EDSC K
Sbjct: 301 LGDIARAKFDFDVVGHYARPEVLSLVVRDHPTTPVTFTSASKKTEDSCNK 350

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BLAST of CsaV3_1G015110 vs. NCBI nr
Match: XP_022927562.1 (bifunctional nitrilase/nitrile hydratase NIT4A [Cucurbita moschata])

HSP 1 Score: 685.3 bits (1767), Expect = 1.1e-193
Identity = 334/349 (95.70%), Postives = 340/349 (97.42%), Query Frame = 0


Query: 1   MALVPTTAPTDSQLIAEVDMGSDSSVMNVRATVVQASTIFYDTPATLDKAERLLAEAAGF 60
           MALVPTTA  D+QLIAEVDMGSD SV  VRATVVQASTIFYDTPATLDKAERLLAEAAGF
Sbjct: 1   MALVPTTAAADTQLIAEVDMGSDVSVPTVRATVVQASTIFYDTPATLDKAERLLAEAAGF 60

Query: 61  GSQLVVFPEAFVGGYPRGSNFGVSIGNRTPKGKEEFRKYHASAIDVPGPEVDRLAAMAGK 120
           GSQLVVFPEAFVGGYPRGSNFGVSIGNRTPKGKEEFRKYHA+AIDVPGPEVDRLAAMAGK
Sbjct: 61  GSQLVVFPEAFVGGYPRGSNFGVSIGNRTPKGKEEFRKYHAAAIDVPGPEVDRLAAMAGK 120

Query: 121 YRVHLVMGVIERDGYTLYCTVLFFDPQGRYLGKHRKVMPTALERIIWGFGDGSTIPVFET 180
           YRVHLVMGVIERDGYTLYCTVLFFDPQGRYLGKHRKVMPTALERIIWGFGDGSTIPVFET
Sbjct: 121 YRVHLVMGVIERDGYTLYCTVLFFDPQGRYLGKHRKVMPTALERIIWGFGDGSTIPVFET 180

Query: 181 SIGKIGAAICWENRMPLLRTAMYAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSAN 240
           SIGKIGAAICWENRMPLLRTAMYAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSAN
Sbjct: 181 SIGKIGAAICWENRMPLLRTAMYAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSAN 240

Query: 241 QFCRRKDYPPPPEYVFSGTEEELTPDSVVCAGGSAIISPSGTILAGPNYDGEALISADLD 300
           QFCRRKDYPPPP+YVFSGTEEEL+PDSVVCAGGS IISPSGTILAGPNYDGEALISADLD
Sbjct: 241 QFCRRKDYPPPPDYVFSGTEEELSPDSVVCAGGSVIISPSGTILAGPNYDGEALISADLD 300

Query: 301 LGEIARAKFDFDVVGHYARPEVLSLVVRDHPTTPVTFTSTSTKVEDSCK 350
           LG+IARAKFDFDVVGHYARPEVLSLVVRDHPTTPVTFTS + K EDSCK
Sbjct: 301 LGDIARAKFDFDVVGHYARPEVLSLVVRDHPTTPVTFTSATAKTEDSCK 349

[Close]

BLAST of CsaV3_1G015110 vs. TAIR10
Match: AT5G22300.1 (nitrilase 4)

HSP 1 Score: 556.2 bits (1432), Expect = 1.4e-158
Identity = 271/343 (79.01%), Postives = 303/343 (88.34%), Query Frame = 0


Query: 6   TTAPTDS--QLIAEVDMGSDSSVMNVRATVVQASTIFYDTPATLDKAERLLAEAAGFGSQ 65
           T AP  +  Q+  E+DM +  S   VRATVVQAST+FYDTPATLDKAERLL+EAA  GSQ
Sbjct: 11  TAAPQTNGHQIFPEIDMSAGDSSSIVRATVVQASTVFYDTPATLDKAERLLSEAAENGSQ 70

Query: 66  LVVFPEAFVGGYPRGSNFGVSIGNRTPKGKEEFRKYHASAIDVPGPEVDRLAAMAGKYRV 125
           LVVFPEAF+GGYPRGS F ++IG+RT KG+++FRKYHASAIDVPGPEV+RLA MA KY+V
Sbjct: 71  LVVFPEAFIGGYPRGSTFELAIGSRTAKGRDDFRKYHASAIDVPGPEVERLALMAKKYKV 130

Query: 126 HLVMGVIERDGYTLYCTVLFFDPQGRYLGKHRKVMPTALERIIWGFGDGSTIPVFETSIG 185
           +LVMGVIER+GYTLYCTVLFFD QG +LGKHRK+MPTALER IWGFGDGSTIPVF+T IG
Sbjct: 131 YLVMGVIEREGYTLYCTVLFFDSQGLFLGKHRKLMPTALERCIWGFGDGSTIPVFDTPIG 190

Query: 186 KIGAAICWENRMPLLRTAMYAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSANQFC 245
           KIGAAICWENRMP LRTAMYAKGIEIYCAPTADSR+TW ASMTHIALEGGCFVLSANQFC
Sbjct: 191 KIGAAICWENRMPSLRTAMYAKGIEIYCAPTADSRETWLASMTHIALEGGCFVLSANQFC 250

Query: 246 RRKDYPPPPEYVFSGTEEELTPDSVVCAGGSAIISPSGTILAGPNYDGEALISADLDLGE 305
           RRKDYP PPEY+FSG+EE LTPDSVVCAGGS+IISP G +LAGPNY GEALI+ADLDLG+
Sbjct: 251 RRKDYPSPPEYMFSGSEESLTPDSVVCAGGSSIISPLGIVLAGPNYRGEALITADLDLGD 310

Query: 306 IARAKFDFDVVGHYARPEVLSLVVRDHPTTPVTFTSTSTKVED 347
           IARAKFDFDVVGHY+RPEV SL +R+HP   V+F  TS  +ED
Sbjct: 311 IARAKFDFDVVGHYSRPEVFSLNIREHPRKAVSF-KTSKVMED 352

[Close]

BLAST of CsaV3_1G015110 vs. TAIR10
Match: AT3G44300.1 (nitrilase 2)

HSP 1 Score: 469.5 bits (1207), Expect = 1.8e-132
Identity = 216/322 (67.08%), Postives = 261/322 (81.06%), Query Frame = 0


Query: 29  VRATVVQASTIFYDTPATLDKAERLLAEAAGFGSQLVVFPEAFVGGYPRGSNFGVSIGNR 88
           VRAT+VQAST++ DTPATL+KA + + EAA  GS+LVVFPEAF+GGYPRG  FG+ +G  
Sbjct: 18  VRATIVQASTVYNDTPATLEKANKFIVEAASKGSELVVFPEAFIGGYPRGFRFGLGVGVH 77

Query: 89  TPKGKEEFRKYHASAIDVPGPEVDRLAAMAGKYRVHLVMGVIERDGYTLYCTVLFFDPQG 148
             +G++EFRKYHASAI VPGPEV++LA +AGK  V+LVMG IE+DGYTLYCT LFF PQG
Sbjct: 78  NEEGRDEFRKYHASAIKVPGPEVEKLAELAGKNNVYLVMGAIEKDGYTLYCTALFFSPQG 137

Query: 149 RYLGKHRKVMPTALERIIWGFGDGSTIPVFETSIGKIGAAICWENRMPLLRTAMYAKGIE 208
           ++LGKHRK+MPT+LER IWG GDGSTIPV++T IGK+GAAICWENRMPL RTA+YAKGIE
Sbjct: 138 QFLGKHRKLMPTSLERCIWGQGDGSTIPVYDTPIGKLGAAICWENRMPLYRTALYAKGIE 197

Query: 209 IYCAPTADSRDTWQASMTHIALEGGCFVLSANQFCRRKDYPPPPEYVFSGTEEELTPDSV 268
           +YCAPTAD    WQ+SM HIA+EGGCFVLSA QFC RKD+P  P+Y+F+   ++  PDS+
Sbjct: 198 LYCAPTADGSKEWQSSMLHIAIEGGCFVLSACQFCLRKDFPDHPDYLFTDWYDDKEPDSI 257

Query: 269 VCAGGSAIISPSGTILAGPNYDGEALISADLDLGEIARAKFDFDVVGHYARPEVLSLVVR 328
           V  GGS IISP G +LAGPN++ E LI+ADLDLG++ARAK  FD VGHY+RP+VL L V 
Sbjct: 258 VSQGGSVIISPLGQVLAGPNFESEGLITADLDLGDVARAKLYFDSVGHYSRPDVLHLTVN 317

Query: 329 DHPTTPVTFTSTSTKVEDSCKK 351
           +HP  PVTF S   K ED   K
Sbjct: 318 EHPKKPVTFISKVEKAEDDSNK 339

[Close]

BLAST of CsaV3_1G015110 vs. TAIR10
Match: AT3G44320.1 (nitrilase 3)

HSP 1 Score: 464.9 bits (1195), Expect = 4.3e-131
Identity = 216/331 (65.26%), Postives = 264/331 (79.76%), Query Frame = 0


Query: 20  MGSDSSVMNVRATVVQASTIFYDTPATLDKAERLLAEAAGFGSQLVVFPEAFVGGYPRGS 79
           +G D S   VR T+VQ+ST++ DTPATLDKAE+ + EAA  G++LV+FPEAF+GGYPRG 
Sbjct: 17  IGVDPS-STVRVTIVQSSTVYNDTPATLDKAEKFIVEAASKGAKLVLFPEAFIGGYPRGF 76

Query: 80  NFGVSIGNRTPKGKEEFRKYHASAIDVPGPEVDRLAAMAGKYRVHLVMGVIERDGYTLYC 139
            FG+++G    +G++EFR YHASAI VPGPEV+RLA +AGK  VHLVMG IE+DGYTLYC
Sbjct: 77  RFGLAVGVHNEEGRDEFRNYHASAIKVPGPEVERLAELAGKNNVHLVMGAIEKDGYTLYC 136

Query: 140 TVLFFDPQGRYLGKHRKVMPTALERIIWGFGDGSTIPVFETSIGKIGAAICWENRMPLLR 199
           T LFF PQG++LGKHRKVMPT+LER IWG GDGSTIPV++T IGKIGAAICWENRMPL R
Sbjct: 137 TALFFSPQGQFLGKHRKVMPTSLERCIWGQGDGSTIPVYDTPIGKIGAAICWENRMPLYR 196

Query: 200 TAMYAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSANQFCRRKDYPPPPEYVFSGT 259
           TA+YAKGIEIYCAPTAD    WQASM HIA+EGGCFVLSA+QFC+R+++P  P+Y+F+  
Sbjct: 197 TALYAKGIEIYCAPTADYSLEWQASMIHIAVEGGCFVLSAHQFCKRREFPEHPDYLFNDI 256

Query: 260 EEELTPDSVVCAGGSAIISPSGTILAGPNYDGEALISADLDLGEIARAKFDFDVVGHYAR 319
            +    D  V  GGS IISP G +LAGPNY+ E L++ADLDLG+IARAK  FDVVGHY++
Sbjct: 257 VDTKEHDPTVSGGGSVIISPLGKVLAGPNYESEGLVTADLDLGDIARAKLYFDVVGHYSK 316

Query: 320 PEVLSLVVRDHPTTPVTFTSTSTKVEDSCKK 351
           P++ +L V +HP  PVTF +   K ED   K
Sbjct: 317 PDIFNLTVNEHPKKPVTFMTKVEKAEDESNK 346

[Close]

BLAST of CsaV3_1G015110 vs. TAIR10
Match: AT3G44310.1 (nitrilase 1)

HSP 1 Score: 451.8 bits (1161), Expect = 3.8e-127
Identity = 207/322 (64.29%), Postives = 258/322 (80.12%), Query Frame = 0


Query: 29  VRATVVQASTIFYDTPATLDKAERLLAEAAGFGSQLVVFPEAFVGGYPRGSNFGVSIGNR 88
           VR T+VQ+ST++ DTPAT+DKAE+ + EAA  G++LV+FPE F+GGYPRG  FG+++G  
Sbjct: 25  VRVTIVQSSTVYNDTPATIDKAEKYIVEAASKGAELVLFPEGFIGGYPRGFRFGLAVGVH 84

Query: 89  TPKGKEEFRKYHASAIDVPGPEVDRLAAMAGKYRVHLVMGVIERDGYTLYCTVLFFDPQG 148
             +G++EFRKYHASAI VPGPEV RLA +A K  V+LVMG IE++GYTLYCTVLFF PQG
Sbjct: 85  NEEGRDEFRKYHASAIHVPGPEVARLADVARKNHVYLVMGAIEKEGYTLYCTVLFFSPQG 144

Query: 149 RYLGKHRKVMPTALERIIWGFGDGSTIPVFETSIGKIGAAICWENRMPLLRTAMYAKGIE 208
           ++LGKHRK+MPT+LER IWG GDGSTIPV++T IGK+GAAICWENRMPL RTA+YAKGIE
Sbjct: 145 QFLGKHRKLMPTSLERCIWGQGDGSTIPVYDTPIGKLGAAICWENRMPLYRTALYAKGIE 204

Query: 209 IYCAPTADSRDTWQASMTHIALEGGCFVLSANQFCRRKDYPPPPEYVFSGTEEELTPDSV 268
           +YCAPTAD    WQ+SM HIA+EGGCFVLSA QFC+RK +P  P+Y+F+   ++   DS+
Sbjct: 205 LYCAPTADGSKEWQSSMLHIAIEGGCFVLSACQFCQRKHFPDHPDYLFTDWYDDKEHDSI 264

Query: 269 VCAGGSAIISPSGTILAGPNYDGEALISADLDLGEIARAKFDFDVVGHYARPEVLSLVVR 328
           V  GGS IISP G +LAGPN++ E L++AD+DLG+IARAK  FD VGHY+RP+VL L V 
Sbjct: 265 VSQGGSVIISPLGQVLAGPNFESEGLVTADIDLGDIARAKLYFDSVGHYSRPDVLHLTVN 324

Query: 329 DHPTTPVTFTSTSTKVEDSCKK 351
           +HP   VTF +   K ED   K
Sbjct: 325 EHPRKSVTFVTKVEKAEDDSNK 346

[Close]

BLAST of CsaV3_1G015110 vs. TAIR10
Match: AT2G27450.2 (nitrilase-like protein 1)

HSP 1 Score: 73.9 bits (180), Expect = 2.1e-13
Identity = 59/210 (28.10%), Postives = 87/210 (41.43%), Query Frame = 0


Query: 109 PEVDRLAAMAGKYRVHLVMGVIERDGYTLYCTVLFFDPQGRYLGKHRKVM----PTALER 168
           P + R+  +A +  V + +   E      Y ++   D  G  LG +RK      P   E+
Sbjct: 102 PTIARMQKLAKELGVVIPVSFFEEANTAHYNSIAIIDADGTDLGIYRKSHIPDGPGYQEK 161

Query: 169 IIWGFGDGSTIPVFETSIGKIGAAICWENRMPLLRTAMYAKGIEIYCAPTA--------- 228
             +  GD +   VF+T   KIG AICW+   P    AM  +G EI   PTA         
Sbjct: 162 FYFNPGD-TGFKVFQTKFAKIGVAICWDQWFPEAARAMVLQGAEILFYPTAIGSEPQDQG 221

Query: 229 -DSRDTWQASMTHIALEGGCFVLSANQFCRRKDYPPPPEYVFSGTEEELTPDSVVCAGGS 288
            DSRD W+  M   A      ++++N+  +               E E  P  +   G S
Sbjct: 222 LDSRDHWRRVMQGHAGANVVPLVASNRIGKEI------------IETEHGPSQITFYGTS 281

Query: 289 AIISPSGTILAGPNYDGEALISADLDLGEI 305
            I  P+G I+A  +   EA++ A  DL  I
Sbjct: 282 FIAGPTGEIVAEADDKSEAVLVAQFDLDMI 298

[Close]

BLAST of CsaV3_1G015110 vs. Swiss-Prot
Match: sp|Q42965|NRL4A_TOBAC (Bifunctional nitrilase/nitrile hydratase NIT4A OS=Nicotiana tabacum OX=4097 GN=NIT4A PE=2 SV=1)

HSP 1 Score: 628.6 bits (1620), Expect = 4.1e-179
Identity = 304/349 (87.11%), Postives = 323/349 (92.55%), Query Frame = 0


Query: 1   MALVPTTAPTDSQLIAEVDMGSDSSVMNVRATVVQASTIFYDTPATLDKAERLLAEAAGF 60
           MALVPT A  +  L AEVDMG +SS   VRATVVQASTIFYDTPATL KAERLLAEAA +
Sbjct: 1   MALVPTPAVNEGPLFAEVDMGDNSSTPTVRATVVQASTIFYDTPATLVKAERLLAEAASY 60

Query: 61  GSQLVVFPEAFVGGYPRGSNFGVSIGNRTPKGKEEFRKYHASAIDVPGPEVDRLAAMAGK 120
           G+QLVVFPEAF+GGYPRGS FGVSIGNRT KGKEEFRKYHASAIDVPGPEVDRLAAMAGK
Sbjct: 61  GAQLVVFPEAFIGGYPRGSTFGVSIGNRTAKGKEEFRKYHASAIDVPGPEVDRLAAMAGK 120

Query: 121 YRVHLVMGVIERDGYTLYCTVLFFDPQGRYLGKHRKVMPTALERIIWGFGDGSTIPVFET 180
           Y+V+LVMGVIERDGYTLYCTVLFFD QG +LGKHRK+MPTALERIIWGFGDGSTIPV++T
Sbjct: 121 YKVYLVMGVIERDGYTLYCTVLFFDSQGHFLGKHRKIMPTALERIIWGFGDGSTIPVYDT 180

Query: 181 SIGKIGAAICWENRMPLLRTAMYAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSAN 240
            +GKIGAAICWENRMPLLRTAMYAKGIEIYCAPTADSRD WQASMTHIALEGGCFVLSAN
Sbjct: 181 PLGKIGAAICWENRMPLLRTAMYAKGIEIYCAPTADSRDVWQASMTHIALEGGCFVLSAN 240

Query: 241 QFCRRKDYPPPPEYVFSGTEEELTPDSVVCAGGSAIISPSGTILAGPNYDGEALISADLD 300
           QFCRRKDYPPPPEYVFSGTEE+LTPDS+VCAGGS IISPSG +LAGPNY GEALISADLD
Sbjct: 241 QFCRRKDYPPPPEYVFSGTEEDLTPDSIVCAGGSVIISPSGAVLAGPNYVGEALISADLD 300

Query: 301 LGEIARAKFDFDVVGHYARPEVLSLVVRDHPTTPVTFTSTSTKVEDSCK 350
           LGEIARAKFDFDVVGHYARPEVLSL+VRDH  +PV+FTSTS+K E S K
Sbjct: 301 LGEIARAKFDFDVVGHYARPEVLSLIVRDHAVSPVSFTSTSSKAESSPK 349

[Close]

BLAST of CsaV3_1G015110 vs. Swiss-Prot
Match: sp|Q42966|NRL4B_TOBAC (Bifunctional nitrilase/nitrile hydratase NIT4B OS=Nicotiana tabacum OX=4097 GN=NIT4B PE=2 SV=1)

HSP 1 Score: 626.7 bits (1615), Expect = 1.5e-178
Identity = 303/349 (86.82%), Postives = 323/349 (92.55%), Query Frame = 0


Query: 1   MALVPTTAPTDSQLIAEVDMGSDSSVMNVRATVVQASTIFYDTPATLDKAERLLAEAAGF 60
           MALVPT    +  + AEVDMG +SS   VRATVVQASTIFYDTPATLDKAERLLAEAA +
Sbjct: 1   MALVPTPVVNEGPMFAEVDMGDNSSTPTVRATVVQASTIFYDTPATLDKAERLLAEAASY 60

Query: 61  GSQLVVFPEAFVGGYPRGSNFGVSIGNRTPKGKEEFRKYHASAIDVPGPEVDRLAAMAGK 120
           G+QLVVFPEAF+GGYPRGS FGVSIGNRT KGKEEFRKYHASAIDVPGPEVDRLAAMAGK
Sbjct: 61  GAQLVVFPEAFIGGYPRGSTFGVSIGNRTAKGKEEFRKYHASAIDVPGPEVDRLAAMAGK 120

Query: 121 YRVHLVMGVIERDGYTLYCTVLFFDPQGRYLGKHRKVMPTALERIIWGFGDGSTIPVFET 180
           Y+V+LVMGVIERDGYTLYCTVLFFD QG YLGKHRK+MPTALERIIWGFGDGSTIPV++T
Sbjct: 121 YKVYLVMGVIERDGYTLYCTVLFFDSQGHYLGKHRKIMPTALERIIWGFGDGSTIPVYDT 180

Query: 181 SIGKIGAAICWENRMPLLRTAMYAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSAN 240
            +GKIGAAICWENRMPLLRTAMYAKGIEIYCAPTADSRD WQASMTHIALEGGCFVLSAN
Sbjct: 181 PLGKIGAAICWENRMPLLRTAMYAKGIEIYCAPTADSRDVWQASMTHIALEGGCFVLSAN 240

Query: 241 QFCRRKDYPPPPEYVFSGTEEELTPDSVVCAGGSAIISPSGTILAGPNYDGEALISADLD 300
           QFCRRKDYPPPPEYVFSGT E+LTPDS+VCAGGS IISPSG +LAGPNY+GEALISADLD
Sbjct: 241 QFCRRKDYPPPPEYVFSGT-EDLTPDSIVCAGGSVIISPSGAVLAGPNYEGEALISADLD 300

Query: 301 LGEIARAKFDFDVVGHYARPEVLSLVVRDHPTTPVTFTSTSTKVEDSCK 350
           LGEIARAKFDFDVVGHYARPEVLSL+VRDH  +PV+FTSTS+K E S K
Sbjct: 301 LGEIARAKFDFDVVGHYARPEVLSLIVRDHAVSPVSFTSTSSKAESSPK 348

[Close]

BLAST of CsaV3_1G015110 vs. Swiss-Prot
Match: sp|Q5QGZ8|NRL4A_LUPAN (Bifunctional nitrilase/nitrile hydratase NIT4A OS=Lupinus angustifolius OX=3871 GN=NIT4A PE=1 SV=1)

HSP 1 Score: 625.2 bits (1611), Expect = 4.5e-178
Identity = 299/349 (85.67%), Postives = 322/349 (92.26%), Query Frame = 0


Query: 1   MALVPTTAPTDSQLIAEVDMGSDSSVMNVRATVVQASTIFYDTPATLDKAERLLAEAAGF 60
           MALV T    D  L AEV+M SD +   VRATVVQASTIFYDTPATLDKAERLLAEAA +
Sbjct: 1   MALVTTPTVNDGPLFAEVNMSSDFNAPTVRATVVQASTIFYDTPATLDKAERLLAEAASY 60

Query: 61  GSQLVVFPEAFVGGYPRGSNFGVSIGNRTPKGKEEFRKYHASAIDVPGPEVDRLAAMAGK 120
           G+Q+VVFPEAF+GGYPRGSNFGVSIGNRT KGKE+FRKYH++AIDVPGPEVDRLAA+AGK
Sbjct: 61  GAQIVVFPEAFIGGYPRGSNFGVSIGNRTAKGKEDFRKYHSAAIDVPGPEVDRLAALAGK 120

Query: 121 YRVHLVMGVIERDGYTLYCTVLFFDPQGRYLGKHRKVMPTALERIIWGFGDGSTIPVFET 180
           Y+V+LVMGVIERDGYTLYCTVLFF  QGRYLGKHRK+MPTALERIIWGFGDGSTIPVFET
Sbjct: 121 YKVYLVMGVIERDGYTLYCTVLFFGAQGRYLGKHRKLMPTALERIIWGFGDGSTIPVFET 180

Query: 181 SIGKIGAAICWENRMPLLRTAMYAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSAN 240
            IGKIGAAICWEN+MPLLRTAMYAKG+EIYCAPTADSR+ WQASMTHIALEGGCFVLSAN
Sbjct: 181 PIGKIGAAICWENKMPLLRTAMYAKGVEIYCAPTADSREVWQASMTHIALEGGCFVLSAN 240

Query: 241 QFCRRKDYPPPPEYVFSGTEEELTPDSVVCAGGSAIISPSGTILAGPNYDGEALISADLD 300
           QFCRR+DYPPPPEYVF GTEE LTPDSVVCAGGS IISPSG +LAGP+Y+GEALISADLD
Sbjct: 241 QFCRRRDYPPPPEYVFEGTEENLTPDSVVCAGGSVIISPSGAVLAGPSYEGEALISADLD 300

Query: 301 LGEIARAKFDFDVVGHYARPEVLSLVVRDHPTTPVTFTSTSTKVEDSCK 350
           LGEIARAKFDFDVVGHY+RPEVLSLVV+DHPT PVTFTS STK+ED  K
Sbjct: 301 LGEIARAKFDFDVVGHYSRPEVLSLVVKDHPTNPVTFTSASTKIEDKTK 349

[Close]

BLAST of CsaV3_1G015110 vs. Swiss-Prot
Match: sp|Q3LRV4|NRL4B_LUPAN (Bifunctional nitrilase/nitrile hydratase NIT4B OS=Lupinus angustifolius OX=3871 GN=NIT4B PE=1 SV=1)

HSP 1 Score: 621.7 bits (1602), Expect = 5.0e-177
Identity = 303/351 (86.32%), Postives = 324/351 (92.31%), Query Frame = 0


Query: 1   MALVPTTAPT--DSQLIAEVDMGSDSSVMNVRATVVQASTIFYDTPATLDKAERLLAEAA 60
           MALV TT PT  D  L AEVDM S  +   VRATVVQASTIFYDTPATLDKAERLL +AA
Sbjct: 1   MALV-TTTPTVNDEPLFAEVDMASYFTSTTVRATVVQASTIFYDTPATLDKAERLLVQAA 60

Query: 61  GFGSQLVVFPEAFVGGYPRGSNFGVSIGNRTPKGKEEFRKYHASAIDVPGPEVDRLAAMA 120
            +G+Q+VVFPEAF+GGYPRGSNFGVSIGNRT KGKEEFRKYH++AIDVPGPEVDRL+AMA
Sbjct: 61  SYGAQIVVFPEAFIGGYPRGSNFGVSIGNRTAKGKEEFRKYHSAAIDVPGPEVDRLSAMA 120

Query: 121 GKYRVHLVMGVIERDGYTLYCTVLFFDPQGRYLGKHRKVMPTALERIIWGFGDGSTIPVF 180
           GKY+V+LVMGVIERDGYTLYCTVLFFD QGRYLGKHRKVMPTALERIIWGFGDGSTIPVF
Sbjct: 121 GKYKVYLVMGVIERDGYTLYCTVLFFDSQGRYLGKHRKVMPTALERIIWGFGDGSTIPVF 180

Query: 181 ETSIGKIGAAICWENRMPLLRTAMYAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLS 240
           +T IGKIGAAICWEN+MPLLRTAMYAKG+EIYCAPTADSRD WQAS THIALEGGCFVLS
Sbjct: 181 QTPIGKIGAAICWENKMPLLRTAMYAKGVEIYCAPTADSRDLWQASTTHIALEGGCFVLS 240

Query: 241 ANQFCRRKDYPPPPEYVFSGTEEELTPDSVVCAGGSAIISPSGTILAGPNYDGEALISAD 300
           ANQFCRRKDYPPPPEYVFSGTEE+LTPDSVV AGGS IISPSG +LAGPNY+GEALISAD
Sbjct: 241 ANQFCRRKDYPPPPEYVFSGTEEDLTPDSVVSAGGSVIISPSGAVLAGPNYEGEALISAD 300

Query: 301 LDLGEIARAKFDFDVVGHYARPEVLSLVVRDHPTTPVTFTSTSTKVEDSCK 350
           LDLGEIARAKFDFDVVGHY+R EVLSL+V+DHPT PVTFTSTSTK+ED  K
Sbjct: 301 LDLGEIARAKFDFDVVGHYSRSEVLSLIVKDHPTNPVTFTSTSTKIEDQTK 350

[Close]

BLAST of CsaV3_1G015110 vs. Swiss-Prot
Match: sp|P46011|NRL4_ARATH (Bifunctional nitrilase/nitrile hydratase NIT4 OS=Arabidopsis thaliana OX=3702 GN=NIT4 PE=1 SV=1)

HSP 1 Score: 556.2 bits (1432), Expect = 2.6e-157
Identity = 271/343 (79.01%), Postives = 303/343 (88.34%), Query Frame = 0


Query: 6   TTAPTDS--QLIAEVDMGSDSSVMNVRATVVQASTIFYDTPATLDKAERLLAEAAGFGSQ 65
           T AP  +  Q+  E+DM +  S   VRATVVQAST+FYDTPATLDKAERLL+EAA  GSQ
Sbjct: 11  TAAPQTNGHQIFPEIDMSAGDSSSIVRATVVQASTVFYDTPATLDKAERLLSEAAENGSQ 70

Query: 66  LVVFPEAFVGGYPRGSNFGVSIGNRTPKGKEEFRKYHASAIDVPGPEVDRLAAMAGKYRV 125
           LVVFPEAF+GGYPRGS F ++IG+RT KG+++FRKYHASAIDVPGPEV+RLA MA KY+V
Sbjct: 71  LVVFPEAFIGGYPRGSTFELAIGSRTAKGRDDFRKYHASAIDVPGPEVERLALMAKKYKV 130

Query: 126 HLVMGVIERDGYTLYCTVLFFDPQGRYLGKHRKVMPTALERIIWGFGDGSTIPVFETSIG 185
           +LVMGVIER+GYTLYCTVLFFD QG +LGKHRK+MPTALER IWGFGDGSTIPVF+T IG
Sbjct: 131 YLVMGVIEREGYTLYCTVLFFDSQGLFLGKHRKLMPTALERCIWGFGDGSTIPVFDTPIG 190

Query: 186 KIGAAICWENRMPLLRTAMYAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSANQFC 245
           KIGAAICWENRMP LRTAMYAKGIEIYCAPTADSR+TW ASMTHIALEGGCFVLSANQFC
Sbjct: 191 KIGAAICWENRMPSLRTAMYAKGIEIYCAPTADSRETWLASMTHIALEGGCFVLSANQFC 250

Query: 246 RRKDYPPPPEYVFSGTEEELTPDSVVCAGGSAIISPSGTILAGPNYDGEALISADLDLGE 305
           RRKDYP PPEY+FSG+EE LTPDSVVCAGGS+IISP G +LAGPNY GEALI+ADLDLG+
Sbjct: 251 RRKDYPSPPEYMFSGSEESLTPDSVVCAGGSSIISPLGIVLAGPNYRGEALITADLDLGD 310

Query: 306 IARAKFDFDVVGHYARPEVLSLVVRDHPTTPVTFTSTSTKVED 347
           IARAKFDFDVVGHY+RPEV SL +R+HP   V+F  TS  +ED
Sbjct: 311 IARAKFDFDVVGHYSRPEVFSLNIREHPRKAVSF-KTSKVMED 352

[Close]

BLAST of CsaV3_1G015110 vs. TrEMBL
Match: tr|A0A0A0LVI0|A0A0A0LVI0_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G168360 PE=4 SV=1)

HSP 1 Score: 713.8 bits (1841), Expect = 1.9e-202
Identity = 350/350 (100.00%), Postives = 350/350 (100.00%), Query Frame = 0


Query: 1   MALVPTTAPTDSQLIAEVDMGSDSSVMNVRATVVQASTIFYDTPATLDKAERLLAEAAGF 60
           MALVPTTAPTDSQLIAEVDMGSDSSVMNVRATVVQASTIFYDTPATLDKAERLLAEAAGF
Sbjct: 1   MALVPTTAPTDSQLIAEVDMGSDSSVMNVRATVVQASTIFYDTPATLDKAERLLAEAAGF 60

Query: 61  GSQLVVFPEAFVGGYPRGSNFGVSIGNRTPKGKEEFRKYHASAIDVPGPEVDRLAAMAGK 120
           GSQLVVFPEAFVGGYPRGSNFGVSIGNRTPKGKEEFRKYHASAIDVPGPEVDRLAAMAGK
Sbjct: 61  GSQLVVFPEAFVGGYPRGSNFGVSIGNRTPKGKEEFRKYHASAIDVPGPEVDRLAAMAGK 120

Query: 121 YRVHLVMGVIERDGYTLYCTVLFFDPQGRYLGKHRKVMPTALERIIWGFGDGSTIPVFET 180
           YRVHLVMGVIERDGYTLYCTVLFFDPQGRYLGKHRKVMPTALERIIWGFGDGSTIPVFET
Sbjct: 121 YRVHLVMGVIERDGYTLYCTVLFFDPQGRYLGKHRKVMPTALERIIWGFGDGSTIPVFET 180

Query: 181 SIGKIGAAICWENRMPLLRTAMYAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSAN 240
           SIGKIGAAICWENRMPLLRTAMYAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSAN
Sbjct: 181 SIGKIGAAICWENRMPLLRTAMYAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSAN 240

Query: 241 QFCRRKDYPPPPEYVFSGTEEELTPDSVVCAGGSAIISPSGTILAGPNYDGEALISADLD 300
           QFCRRKDYPPPPEYVFSGTEEELTPDSVVCAGGSAIISPSGTILAGPNYDGEALISADLD
Sbjct: 241 QFCRRKDYPPPPEYVFSGTEEELTPDSVVCAGGSAIISPSGTILAGPNYDGEALISADLD 300

Query: 301 LGEIARAKFDFDVVGHYARPEVLSLVVRDHPTTPVTFTSTSTKVEDSCKK 351
           LGEIARAKFDFDVVGHYARPEVLSLVVRDHPTTPVTFTSTSTKVEDSCKK
Sbjct: 301 LGEIARAKFDFDVVGHYARPEVLSLVVRDHPTTPVTFTSTSTKVEDSCKK 350

[Close]

BLAST of CsaV3_1G015110 vs. TrEMBL
Match: tr|A0A1S3CLB4|A0A1S3CLB4_CUCME (bifunctional nitrilase/nitrile hydratase NIT4A OS=Cucumis melo OX=3656 GN=LOC103501743 PE=4 SV=1)

HSP 1 Score: 701.4 bits (1809), Expect = 1.0e-198
Identity = 343/350 (98.00%), Postives = 345/350 (98.57%), Query Frame = 0


Query: 1   MALVPTTAPTDSQLIAEVDMGSDSSVMNVRATVVQASTIFYDTPATLDKAERLLAEAAGF 60
           MALVPTTAPTD QLIAEVDMG+DSSVM VRATVVQASTIFYDTPATLDKAERLLAEAAGF
Sbjct: 1   MALVPTTAPTDPQLIAEVDMGTDSSVMTVRATVVQASTIFYDTPATLDKAERLLAEAAGF 60

Query: 61  GSQLVVFPEAFVGGYPRGSNFGVSIGNRTPKGKEEFRKYHASAIDVPGPEVDRLAAMAGK 120
           GSQLVVFPEAFVGGYPRGSNFGVSIGNRTPKGKEEFRKYHA+AIDVPGPEVDRLAAMAGK
Sbjct: 61  GSQLVVFPEAFVGGYPRGSNFGVSIGNRTPKGKEEFRKYHAAAIDVPGPEVDRLAAMAGK 120

Query: 121 YRVHLVMGVIERDGYTLYCTVLFFDPQGRYLGKHRKVMPTALERIIWGFGDGSTIPVFET 180
           YRVHLVMGVIERDGYTLYCTVLFFDPQGRYLGKHRKVMPTALERIIWGFGDGSTIPVFET
Sbjct: 121 YRVHLVMGVIERDGYTLYCTVLFFDPQGRYLGKHRKVMPTALERIIWGFGDGSTIPVFET 180

Query: 181 SIGKIGAAICWENRMPLLRTAMYAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSAN 240
           SIGKIGAAICWENRMPLLRTAMYAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSAN
Sbjct: 181 SIGKIGAAICWENRMPLLRTAMYAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSAN 240

Query: 241 QFCRRKDYPPPPEYVFSGTEEELTPDSVVCAGGSAIISPSGTILAGPNYDGEALISADLD 300
           QFCRRKDYPPPPEYVFSGTEEELTPDSVVCAGGSAIISPSGTILAGPNYDGEALISADLD
Sbjct: 241 QFCRRKDYPPPPEYVFSGTEEELTPDSVVCAGGSAIISPSGTILAGPNYDGEALISADLD 300

Query: 301 LGEIARAKFDFDVVGHYARPEVLSLVVRDHPTTPVTFTSTSTKVEDSCKK 351
           LGEIARAKFDFDVVGHYARPEVLSLVVRDHP  PVTFTSTSTK EDSCKK
Sbjct: 301 LGEIARAKFDFDVVGHYARPEVLSLVVRDHPAIPVTFTSTSTKAEDSCKK 350

[Close]

BLAST of CsaV3_1G015110 vs. TrEMBL
Match: tr|A0A2P5CW35|A0A2P5CW35_9ROSA (Nitrilase/cyanide hydratase OS=Trema orientalis OX=63057 GN=TorNIT1 PE=4 SV=1)

HSP 1 Score: 648.7 bits (1672), Expect = 7.7e-183
Identity = 313/347 (90.20%), Postives = 329/347 (94.81%), Query Frame = 0


Query: 1   MALVPTTAPTDSQLIAEVDMGSDSSVMNVRATVVQASTIFYDTPATLDKAERLLAEAAGF 60
           MALVP+    D+ L AEVDMG+DSS  +VRATVVQAST+FYDTPATLDKAERLLAEAAG+
Sbjct: 1   MALVPSAPLNDAPLFAEVDMGADSSAPSVRATVVQASTVFYDTPATLDKAERLLAEAAGY 60

Query: 61  GSQLVVFPEAFVGGYPRGSNFGVSIGNRTPKGKEEFRKYHASAIDVPGPEVDRLAAMAGK 120
           GSQLVVFPEAFVGGYPRGSNFGV+IGNRTPKGKEEFRKYHASAIDVPGPEVDRLAAMAGK
Sbjct: 61  GSQLVVFPEAFVGGYPRGSNFGVTIGNRTPKGKEEFRKYHASAIDVPGPEVDRLAAMAGK 120

Query: 121 YRVHLVMGVIERDGYTLYCTVLFFDPQGRYLGKHRKVMPTALERIIWGFGDGSTIPVFET 180
           Y+V+LVMGVIERDGYTLYCTVLFFD QGRYLGKHRKVMPTALERIIWGFGDGSTIPVFET
Sbjct: 121 YKVYLVMGVIERDGYTLYCTVLFFDSQGRYLGKHRKVMPTALERIIWGFGDGSTIPVFET 180

Query: 181 SIGKIGAAICWENRMPLLRTAMYAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSAN 240
            IGKIGAAICWENRMPLLRTAMYAKG+EIYCAPTAD+R+ WQASMTHIALEG CFVLSAN
Sbjct: 181 PIGKIGAAICWENRMPLLRTAMYAKGVEIYCAPTADAREVWQASMTHIALEGACFVLSAN 240

Query: 241 QFCRRKDYPPPPEYVFSGTEEELTPDSVVCAGGSAIISPSGTILAGPNYDGEALISADLD 300
           QFCRRKDYPPPPEYVFSGTE ELTPDSVVCAGGS IISPSGT+LAGPNYDGEALISADLD
Sbjct: 241 QFCRRKDYPPPPEYVFSGTEGELTPDSVVCAGGSVIISPSGTVLAGPNYDGEALISADLD 300

Query: 301 LGEIARAKFDFDVVGHYARPEVLSLVVRDHPTTPVTFTSTSTKVEDS 348
           LGEIARAKFDFDVVGHY+RPEVLSL+VRDHPT PVTFTS+S K E S
Sbjct: 301 LGEIARAKFDFDVVGHYSRPEVLSLIVRDHPTNPVTFTSSSLKTESS 347

[Close]

BLAST of CsaV3_1G015110 vs. TrEMBL
Match: tr|A0A2P5E021|A0A2P5E021_PARAD (Nitrilase/cyanide hydratase OS=Parasponia andersonii OX=3476 GN=PanNIT1 PE=4 SV=1)

HSP 1 Score: 645.2 bits (1663), Expect = 8.5e-182
Identity = 310/347 (89.34%), Postives = 328/347 (94.52%), Query Frame = 0


Query: 1   MALVPTTAPTDSQLIAEVDMGSDSSVMNVRATVVQASTIFYDTPATLDKAERLLAEAAGF 60
           MALVP+    D+ L AEVDMG+DSS   VRATVVQAST++YDTPATLDKAERLLAEAAG+
Sbjct: 1   MALVPSAPLNDAPLFAEVDMGADSSASTVRATVVQASTVYYDTPATLDKAERLLAEAAGY 60

Query: 61  GSQLVVFPEAFVGGYPRGSNFGVSIGNRTPKGKEEFRKYHASAIDVPGPEVDRLAAMAGK 120
           GSQLVVFPEAFVGGYPRGSNFGV+IGNRTPKGKEEFRKYHASAIDVPGPEVD LAAMAGK
Sbjct: 61  GSQLVVFPEAFVGGYPRGSNFGVTIGNRTPKGKEEFRKYHASAIDVPGPEVDLLAAMAGK 120

Query: 121 YRVHLVMGVIERDGYTLYCTVLFFDPQGRYLGKHRKVMPTALERIIWGFGDGSTIPVFET 180
           Y+V+LVMGVIERDGYTLYCTVLFFD QGRY+GKHRKVMPTALERIIWGFGDGSTIPVFET
Sbjct: 121 YKVYLVMGVIERDGYTLYCTVLFFDSQGRYMGKHRKVMPTALERIIWGFGDGSTIPVFET 180

Query: 181 SIGKIGAAICWENRMPLLRTAMYAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSAN 240
            IGKIGAAICWENRMPLLRTAMYAKG+EIYCAPTAD+R+ WQASMTHIA+EGGCFVLSAN
Sbjct: 181 PIGKIGAAICWENRMPLLRTAMYAKGVEIYCAPTADAREVWQASMTHIAVEGGCFVLSAN 240

Query: 241 QFCRRKDYPPPPEYVFSGTEEELTPDSVVCAGGSAIISPSGTILAGPNYDGEALISADLD 300
           QFCRRKDYPPPPEYVFSGTE ELTPDSVVCAGGS IISPSGT+LAGPNYDGEALISADLD
Sbjct: 241 QFCRRKDYPPPPEYVFSGTEGELTPDSVVCAGGSVIISPSGTVLAGPNYDGEALISADLD 300

Query: 301 LGEIARAKFDFDVVGHYARPEVLSLVVRDHPTTPVTFTSTSTKVEDS 348
           LGEIARAKFDFDVVGHY+RPEVLSL+VRDHPT PVTFTS+S K E S
Sbjct: 301 LGEIARAKFDFDVVGHYSRPEVLSLIVRDHPTNPVTFTSSSLKTESS 347

[Close]

BLAST of CsaV3_1G015110 vs. TrEMBL
Match: tr|A0A0R0HW00|A0A0R0HW00_SOYBN (Uncharacterized protein OS=Glycine max OX=3847 GN=100778110 PE=4 SV=1)

HSP 1 Score: 637.9 bits (1644), Expect = 1.4e-179
Identity = 309/351 (88.03%), Postives = 328/351 (93.45%), Query Frame = 0


Query: 1   MALVPTTAPT--DSQLIAEVDMGSDSSVMNVRATVVQASTIFYDTPATLDKAERLLAEAA 60
           MALV TT PT  +  L AEVDMGSD +   VRATVVQASTIFYDTPATLDKAERLLAEAA
Sbjct: 1   MALV-TTNPTINEGPLFAEVDMGSDFNAPTVRATVVQASTIFYDTPATLDKAERLLAEAA 60

Query: 61  GFGSQLVVFPEAFVGGYPRGSNFGVSIGNRTPKGKEEFRKYHASAIDVPGPEVDRLAAMA 120
            +GSQLVVFPEAFVGGYPRGS FG+SIGNRT KG+EEFRKYH++AIDVPGPEVDRLAAMA
Sbjct: 61  SYGSQLVVFPEAFVGGYPRGSAFGLSIGNRTAKGREEFRKYHSAAIDVPGPEVDRLAAMA 120

Query: 121 GKYRVHLVMGVIERDGYTLYCTVLFFDPQGRYLGKHRKVMPTALERIIWGFGDGSTIPVF 180
           GKY+VHLVMGVIERDGYTLYCTVLFFD QG YLGKHRK+MPTALER+IWGFGDGSTIPVF
Sbjct: 121 GKYKVHLVMGVIERDGYTLYCTVLFFDSQGHYLGKHRKIMPTALERVIWGFGDGSTIPVF 180

Query: 181 ETSIGKIGAAICWENRMPLLRTAMYAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLS 240
           ET +GKIGAAICWENRMPLLRTAMYAKG+EIYCAPTADSRD WQASMTHIALEGGCFVLS
Sbjct: 181 ETPVGKIGAAICWENRMPLLRTAMYAKGVEIYCAPTADSRDVWQASMTHIALEGGCFVLS 240

Query: 241 ANQFCRRKDYPPPPEYVFSGTEEELTPDSVVCAGGSAIISPSGTILAGPNYDGEALISAD 300
           ANQFCRRKDYPPPPEYVF+GTEE+LTPDSVVCAGGS IISPSG +LAGPNYDGEALISAD
Sbjct: 241 ANQFCRRKDYPPPPEYVFAGTEEDLTPDSVVCAGGSVIISPSGAVLAGPNYDGEALISAD 300

Query: 301 LDLGEIARAKFDFDVVGHYARPEVLSLVVRDHPTTPVTFTSTSTKVEDSCK 350
           LDLGEIARAKFDFDVVGHY+RPEVLSL+V+DHPT PVTFTSTSTK+ED  K
Sbjct: 301 LDLGEIARAKFDFDVVGHYSRPEVLSLIVKDHPTNPVTFTSTSTKIEDKTK 350

The following BLAST results are available for this feature:

BLAST of CsaV3_1G015110 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004139500.1	2.9e-202	100.00	PREDICTED: bifunctional nitrilase/nitrile hydratase NIT4A [Cucumis sativus] >KGN...	[more]
XP_008463648.1	1.5e-198	98.00	PREDICTED: bifunctional nitrilase/nitrile hydratase NIT4A [Cucumis melo]	[more]
XP_023520715.1	9.2e-196	96.57	bifunctional nitrilase/nitrile hydratase NIT4A [Cucurbita pepo subsp. pepo]	[more]
XP_022973146.1	6.6e-194	95.71	bifunctional nitrilase/nitrile hydratase NIT4A [Cucurbita maxima]	[more]
XP_022927562.1	1.1e-193	95.70	bifunctional nitrilase/nitrile hydratase NIT4A [Cucurbita moschata]	[more]

BLAST of CsaV3_1G015110 vs. TAIR10


Match Name	E-value	Identity	Description
AT5G22300.1	1.4e-158	79.01	nitrilase 4	[more]
AT3G44300.1	1.8e-132	67.08	nitrilase 2	[more]
AT3G44320.1	4.3e-131	65.26	nitrilase 3	[more]
AT3G44310.1	3.8e-127	64.29	nitrilase 1	[more]
AT2G27450.2	2.1e-13	28.10	nitrilase-like protein 1	[more]

BLAST of CsaV3_1G015110 vs. Swiss-Prot


Match Name	E-value	Identity	Description
sp\|Q42965\|NRL4A_TOBAC	4.1e-179	87.11	Bifunctional nitrilase/nitrile hydratase NIT4A OS=Nicotiana tabacum OX=4097 GN=N...	[more]
sp\|Q42966\|NRL4B_TOBAC	1.5e-178	86.82	Bifunctional nitrilase/nitrile hydratase NIT4B OS=Nicotiana tabacum OX=4097 GN=N...	[more]
sp\|Q5QGZ8\|NRL4A_LUPAN	4.5e-178	85.67	Bifunctional nitrilase/nitrile hydratase NIT4A OS=Lupinus angustifolius OX=3871 ...	[more]
sp\|Q3LRV4\|NRL4B_LUPAN	5.0e-177	86.32	Bifunctional nitrilase/nitrile hydratase NIT4B OS=Lupinus angustifolius OX=3871 ...	[more]
sp\|P46011\|NRL4_ARATH	2.6e-157	79.01	Bifunctional nitrilase/nitrile hydratase NIT4 OS=Arabidopsis thaliana OX=3702 GN...	[more]

BLAST of CsaV3_1G015110 vs. TrEMBL


Match Name	E-value	Identity	Description
tr\|A0A0A0LVI0\|A0A0A0LVI0_CUCSA	1.9e-202	100.00	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G168360 PE=4 SV=1	[more]
tr\|A0A1S3CLB4\|A0A1S3CLB4_CUCME	1.0e-198	98.00	bifunctional nitrilase/nitrile hydratase NIT4A OS=Cucumis melo OX=3656 GN=LOC103...	[more]
tr\|A0A2P5CW35\|A0A2P5CW35_9ROSA	7.7e-183	90.20	Nitrilase/cyanide hydratase OS=Trema orientalis OX=63057 GN=TorNIT1 PE=4 SV=1	[more]
tr\|A0A2P5E021\|A0A2P5E021_PARAD	8.5e-182	89.34	Nitrilase/cyanide hydratase OS=Parasponia andersonii OX=3476 GN=PanNIT1 PE=4 SV=...	[more]
tr\|A0A0R0HW00\|A0A0R0HW00_SOYBN	1.4e-179	88.03	Uncharacterized protein OS=Glycine max OX=3847 GN=100778110 PE=4 SV=1	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **Molecular Function**
Term	Definition
GO:0016810	hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds

Vocabulary: **Biological Process**
Term	Definition
GO:0006807	nitrogen compound metabolic process

Vocabulary: **INTERPRO**
Term	Definition
IPR000132	Nitrilase/CN_hydratase_CS
IPR003010	C-N_Hydrolase
IPR036526	C-N_Hydrolase_sf

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006807	nitrogen compound metabolic process
biological_process	GO:0050898	nitrile metabolic process
biological_process	GO:0019500	cyanide catabolic process
biological_process	GO:0051410	detoxification of nitrogen compound
cellular_component	GO:0005575	cellular_component
cellular_component	GO:0005886	plasma membrane
molecular_function	GO:0016810	hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds
molecular_function	GO:0047558	3-cyanoalanine hydratase activity
molecular_function	GO:0047427	cyanoalanine nitrilase activity
molecular_function	GO:0000257	nitrilase activity

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CsaV3_1G015110.1	CsaV3_1G015110.1	mRNA

InterPro

Analysis Name: InterPro Annotations of cucumber chineselong genome (v3)
Date Performed: 2019-03-04


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR036526	Carbon-nitrogen hydrolase superfamily	GENE3D	G3DSA:3.60.110.10		coord: 20..313 e-value: 2.4E-105 score: 353.6
IPR036526	Carbon-nitrogen hydrolase superfamily	SUPERFAMILY	SSF56317	Carbon-nitrogen hydrolase	coord: 29..327
IPR003010	Carbon-nitrogen hydrolase	PFAM	PF00795	CN_hydrolase	coord: 30..308 e-value: 1.0E-69 score: 234.7
IPR003010	Carbon-nitrogen hydrolase	PROSITE	PS50263	CN_HYDROLASE	coord: 29..301 score: 58.056
None	No IPR available	PANTHER	PTHR23088	NITRILASE-RELATED	coord: 19..343
None	No IPR available	PANTHER	PTHR23088:SF31	SUBFAMILY NOT NAMED	coord: 19..343
None	No IPR available	CDD	cd07564	nitrilases_CHs	coord: 29..329 e-value: 2.83269E-161 score: 455.409
IPR000132	Nitrilase/cyanide hydratase, conserved site	PROSITE	PS00921	NITRIL_CHT_2	coord: 186..199
IPR000132	Nitrilase/cyanide hydratase, conserved site	PROSITE	PS00920	NITRIL_CHT_1	coord: 61..76

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
CsaV3_1G015110	CSPI01G15000	Wild cucumber (PI 183967)	cpicucB001
CsaV3_1G015110	Cucsa.319690	Cucumber (Gy14) v1	cgycucB496
CsaV3_1G015110	CmaCh13G003490	Cucurbita maxima (Rimu)	cmacucB0253
CsaV3_1G015110	CmoCh13G003800	Cucurbita moschata (Rifu)	cmocucB0239
CsaV3_1G015110	Cp4.1LG00g01690	Cucurbita pepo (Zucchini)	cpecucB0003
CsaV3_1G015110	CsGy1G014790	Cucumber (Gy14) v2	cgybcucB002
CsaV3_1G015110	Carg26503	Silver-seed gourd	carcucB1027
CsaV3_1G015110	Cla011060	Watermelon (97103) v1	cucwmB049
CsaV3_1G015110	MELO3C025585	Melon (DHL92) v3.5.1	cucmeB030
CsaV3_1G015110	ClCG01G011750	Watermelon (Charleston Gray)	cucwcgB024
CsaV3_1G015110	MELO3C025585.2	Melon (DHL92) v3.6.1	cucmedB009
CsaV3_1G015110	Cla97C01G011330	Watermelon (97103) v2	cucwmbB008
CsaV3_1G015110	Bhi08G000022	Wax gourd	cucwgoB051

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
CsaV3_1G015110	Cucumber (Chinese Long) v3	cuccucB023
CsaV3_1G015110	Cucumber (Chinese Long) v3	cuccucB046
CsaV3_1G015110	Silver-seed gourd	carcucB0000
CsaV3_1G015110	Cucurbita maxima (Rimu)	cmacucB0476
CsaV3_1G015110	Cucurbita maxima (Rimu)	cmacucB0694
CsaV3_1G015110	Cucurbita maxima (Rimu)	cmacucB0962
CsaV3_1G015110	Cucurbita moschata (Rifu)	cmocucB0460
CsaV3_1G015110	Cucurbita moschata (Rifu)	cmocucB0951
CsaV3_1G015110	Cucurbita pepo (Zucchini)	cpecucB0024
CsaV3_1G015110	Cucurbita pepo (Zucchini)	cpecucB1044
CsaV3_1G015110	Bottle gourd (USVL1VR-Ls)	cuclsiB005
CsaV3_1G015110	Bottle gourd (USVL1VR-Ls)	cuclsiB021
CsaV3_1G015110	Bottle gourd (USVL1VR-Ls)	cuclsiB033
CsaV3_1G015110	Melon (DHL92) v3.5.1	cucmeB016
CsaV3_1G015110	Melon (DHL92) v3.5.1	cucmeB072
CsaV3_1G015110	Watermelon (Charleston Gray)	cucwcgB030
CsaV3_1G015110	Watermelon (97103) v1	cucwmB009
CsaV3_1G015110	Watermelon (97103) v1	cucwmB074
CsaV3_1G015110	Watermelon (97103) v2	cucwmbB017
CsaV3_1G015110	Watermelon (97103) v2	cucwmbB025