Bhi08G000022 (gene) Wax gourd | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Bhi08G000022
Type	gene
Organism	Benincasa hispida (Wax gourd)
Description	Nitrilase
Location	chr8 : 698602 .. 709070 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCGCTTGTGCCAACCACGGCCTCCAACGATGCGAGCCAACTGATAGCGGAGGTGGACATGGGCTCCGATTCATCTGTACTCACTGTTCGAGCCACAGTCGTTCAGGCCTCCACCATCTTCTATGACACTCCCGCCACTCTAGGTTCCACTTTCAATTTGCTCCTTATCTACTTCTCTTGCTGCTGTTTTCTTGGCTCGCAATTTTCATGTCAGACTCTGGAACGAGTTCCTGATTGTTTTTGTTTGTTTTCTTAGAAGCTTGTTAGTTGTTTTTGACTTTTTTTTTGGTTGGTTTTCTTTAATCTTAGAAGTTGCTTCCCTTCCATCAGCAGATTTTGGTAGTATAGTTCAATTAGTTTAAGCTATTACATTACATCCTCGATTAGTTCCAATTTTTATTGGTTTTCTTGTTTTCGATCGATATGAATTTGGTATTGTCGCCTAACATTGTGCTTCTGGTTGTTTGGTTCTCCTTAATTTCAGTCGTTTGTAGAAATTGAAGAGGAAACGTACTCATGAAATACGAAGGCTTAAAATGATTTAATTCGAAGATTCCAAAACCCATTAACAGAGTTGGGGATTATCACTCGTAGATGACAAATATTTTAGTATACTATAAGCCAGCTACTAGCCTATGATCATGTGGGCCTCCCCCCCCCCCCTCATCTGTAGTGGTAAAAAGAAATCTAACGCTGGTTTACATGAACAGAAAAGGCTGAGAGGTTGTTGGCTGAAGCTGCTGGATTTGGTTCCCAGCTAGTTGTATTTCCAGAAGCCTTTGTTGGTGGTTATCCCCGTGGATCAAATTTTGGTGTTTGCATTGGGAATAGAACTCCCAAGGGGAAAGAGGAATTTCGTAAATATCACGCAGCAGCGATCGATGTACCTGGTTAGTCTTTGTTTCCAGGTTGGTTGCTCCTGTAAAGAGCAGCCAGGGATAATTTCAGGTTAATTGAGTGTTGCTAGGTTTGTCTCCAGGTTTGTTCAACTCAATTATGGTTGCTCCTGTAAAGAAAAAAAAAAAATCTTAGCGTTTTACCATGGATAATTTCAGGTTCAAAGAATGTTGCTAGGTTCATTGTATAATACTCATTCTAGAAGGCTGACTTAATTTCTCTCCGAGTCTCTATCAAACTTTATTATCCGGTCATATATTAATATTAAAGTCTTACAACGTACCATACATGTTCTGTTCTTTATCATCGTACAATCTTCAAATGGATATTAGCGAATTAGGACAGTAAAATAAGGAGCTAGTGATGCCAGTCTATTCCAATTGAAATACAACGAGATTGTTGTTCATGAAATGTGAAATGGGACTTTAGTCTGTTTTAATGACTCCTGCAATTATCAATTAATGAATGAGAAACAAGATTGCTTCAAAAATCTGCTGGCATTTCTTAGTTCTATTAATTTCAAAACCTTCATACTTTTGCTTACATCTAGACATAATATTTTGAAGTGCTGAAAGCGGCAGTTTCTGTCTTAGGTGCTGTTTAGTTCTGTTTTGAAATTCTTTTTTGCTAAGCCTGGGTTTATGTATGACGTAGATTTACTTCCATTCATCCTAATACAATCTGGTTGGTCTTGTTTTTACTTCCATTCATCTAAGTAAAATCTCGTTTTCTCACAACTAATTGTCTAAAACCATTCTTATTCACCTATAAGGTCATACATCACAACCACAAAAGAAAGTGCTCGAAGTGGAATGGCTGCGGTTCTAATATATTTAAATGATTAATATTTTGATGACTATAGACGGTGATGCATGCAGGTCCTGAAGTCGATCGATTGGCAGCAATGGCTGGAAAGTATAGGGTGCATTTGGTAATGGGGGTGATAGAGAGAGATGGCTACACACTGTACTGTACTATCCTATTTTTTGATCCTCAAGGTCGTTACTTGGGTAAGCACAGGAAATTGATGCCAACTGCATTGGAGCGGCTAATCTGGGGGTTTGGTGATGGATCAACAATTCCTGTTTTTGAGACTTCAATTGGAAAGATAGGTGCTGCCATTTGTTGGGAAAACAGAATGCCACTCCTAAGGACAGCTTTGTATGCAAAAGGTTAGTTATTGGCTGGGAATAATGAAATTCCTGACCGATTGTTTATTTCAAGTCCAGTTTCAGTCTTTCGAAACTTAAAGTAGATAAAAGTACAAAAATCTTGACGAGAAACAAGCAAAAGTAGCCTCATATGAAAACTATTAAAGACTGAAGAGGCTTTAGTTGTTACAAGATGCTAAAATGATGGTCAAACTCCAGAGTTGGCGATTAGCGTATAGTGTGGTGCTCCACTATTTTACGGATAATGGTCTATTTTTTGTTCAGCTTCTGTTCTCAGAATATACTTGGTTGGTTAATGATATTATGCACAACCCATTTTTTTCCTTTTTGTTTCTCCTCTTCTATATTTCTGTTTCAACTACATCAAGTTAAAAAGTCCTAAACCAGATGAAAATTCTCATATAAAAGAAGGTTTCACATGCTTAAAGTTCTACTAATACGTTAAAGATAGAAGTTTGTTAGGAACCAAGATTAGGGATGCCTTTGTCCCACATTGGTTAGAATGGGATGACCAATGTGGTACTTAAGTGGCTTAGCTCTCCCACCTCAATAGCTGACTTTTGGGGTGTGGTTATCCAAGGTGCTTAAGTACCTAACAAATTTTTTATTTCTTCAAAGTTACCTAACTTCTATCCACGCATTCTGTTTCTTTTGCTTAAAAGAAGTTGCTCACTTGAAATTGAATTATCTTGATTGTAGGCATTGAAATATATTGTGCACCTACCGCTGATTCTCGGGATACATGGCAAGCATCAATGACCCATATTGCTTTGGAGGGTGGATGTTTTGTTTTGTCCGCCAACCAGTTCTGCCGAAGAAAAGACTACCCTCCTCCACCAGAGTATGTATTTTCTGGCACAGAAGAGGAACTGAGCCCAGATTCTGTTGTTTGTGCTGGAGGTAGTGCCATCATATCACCTTCAGGGACAATCCTAGCTGGACCCAATTACGATGGAGAGGCACTTATATCTGCAGATCTGGGTATGTCATCTCGTCCTTCTGCTTATAGTGATCAGAGCTTTAGTTGTGCACTCCAACATTATCATATTTAGTGGTTGATATGAAAAGGTTTAATCTTAAGGAACCTACTGTTCGATTTGAGTGTTTTCCTTCTTTAAACGAGTCCTAGGATTCTGTCTCCTGGATGATCTAATGCTCCTGACAGTTAACGAGTCTGAAAACCTTCATTCCAAGTTCTTATTCTCTGCTCAGCTCTTTTTAGTGTCAATACGAGTGAATGTCCACTAGGATTCAGTTGAACAAAATGGGTAAGGATCAACCAACCATTTTAAGAGAGGGAGGGCCTATCTCTCTTGCTGACTAAGAAGTTCCTTGGAGAATGTGGTGGTGTTATTGAACCTTACATGCCTTGAGTTGTGAAACTTTTTCATCTAGATCTGTGGAGCCTAAGAAGTACGGACACTTTAGTTTGGCTAGCTTGCCTGTATCCGACACGTGTCGGACACTCCGACACTCTGACATTTGTTGGACACGTATTGGACACTTGTTAGTGCAACAATTGTATTAGACATGCATAGAATACTTGTTGAGTAAACTAAAAAGACACATATATGACAATAATAATAACTTTTGAATGTGCAATATGTCAAATTAAGTTTTTTTTTTTCAGTTTATAAATGCATTAACCCATTTACTTTGATTTTCTTTTGGTATAAAAATGGTATATATTTTTAAAAATTCATATTTTAATAAGCATGTCCTTGCCATTTCGTGTCCTAGATTTTTAATATATGGCATGTCACCACGTCTGTCATGTCGTATCTGTATCTGTGCTTCTTAAGTGTGAGCAAAGGTCCATCCTCGAATTCCTAAGTTAAGCCTCTTACCTAGGTAGAAGGGTTTTTGTAAAGGTTCTACTTGATAATATTAATAGAAATCTCAAAATTACATAGACAAGCCTAGAACAACCATCTGTTCTAGACGATGACAATCTTCATCCACACTGATATCACTGATATGCTAATCACAAAGAACATACACAAAAGTCTACATAATCCCAAAAGTAGCCAAACACTAACTTAAATATTGAAGGCTAAATACAACCGTAACAAAAGCCCAAGAACGGCCCAATCAAGTCCCACGAGGGAATGTCTCCGATCTACGCCTTCTAACATATCTATGCACTATTGGGGGCTTCACAGTTTTCTTTCACCACTAAGGTTGACTTCTATTTTTCCGCAAGCACGGTCCAAGATTATTGCAGAACGTGAGATATTGAGCTCCTAATATTTGGTAGGTCAAGAAAACAAGAAGCAATAAATCAAGACAGAGTTATCGGATTTTTAACCTCCAGATGGGCTTCCTTTTTATTTATTTATTTATTTATTTTAATTAATTAAAAGAAAAAAACCCTCCTAAGAACTGTCAGTAATGCTTGGTACTTTAACTTTTAATAAGAGACTGGTCTTCAAGCCAGAAATTTTCTTAAATGAATACAGGACTGAAGATGCACATTCAAATCTCCCAAGCAAAAGCCTGCAAATGAAGGTTAGAGACTATAAAGCATAGACATGGATACAAATACGAGATACGGATACGAAAATATGCCAATTTATAAAAAAATAGAATATTGATACGTTGAAGATACATTTTCTTTTCCTTAAGAAAAATCTAGAATATAGATAAATTTAGCATACAAGTTAATGAAAATAGTACAAAATACAATTCAAACATTGAAATACAATACAAAAAACATCTAAGATGTTGAAGTAAAATAATCAATAAAATGTAATAAAAAAGTGACTCGTATTGATCAAGGGAGAAGAAGATTAGAGAGAGAAGTATGAAGATAAACGAAGAAATTCTTTGTTGAAATGTTGAAGATATTTAAGTGGGTTTTATTTTAGAGGACTTTGTGGAGACTCAAATGTGAAGATGAAGATTAGATGGATTGTTAAAAATGTGGTTCGCAATATACTTTTAAATGATTTCTGGAATTTGATAGAAGTGATGCTCTCCCCTCTCTCCTCCTTCCCTCCCTTCCCCCACGCCCCCCCTTCCGACCACCTCGTCGGACCTCACCATGCCCTCCCTAAACTTCCCTCCTTCTGACCGCCTCCCTCACCCCTCTCCCTCCAACCTTCCTCCGGTTTCCATTATCCCTCTCTCACTCTATTTCTGCCCTGCTTTCAGCCATGGCTTTGCTCTCTTGCCGTATCCTTGACCACGCCTTCCTTTTGGAGCAAGAAGGATTGAACTTCCTTTTAAAAGACATAAAAAAAGGACAGTCCATTCTTCTCTCCCTAACCTCAACTACTTGGCTTCTTGAGAGTTTTCGAAGACTATTGTATGGTTCCACCTTTGAGTTTTTCCTTCTAAAGGAAAGAGTTGATCAAAGCTTCATGAGGCTTGACCAAATTTTGTGCAAAGGAGAGTTGGTTTGTTGAATATGCTGTTTGGCCAATCACAGGAGGAAGAAGGAATATCTACATCCCGTTTGGCAATGAAAAAGCAGGCTGGCAAGACTTCAAGGAGATGATCAATAGATGCATCCAACACTTCAAAAAATCCCCTGCTCTCCAGGTTAGTTCTTCCTCATCTCTTCCCTCTACTAAGCTTAGTCCAACCAGCCGTACCAGCCTTGTTCCACCTCCCTGTGAGAACTCTGCCCTCCCACCCCCCTCTCCTCCACATGTGAAGAAACAGAGCATCCCTGTTGTCTGGTTAAAGAAGAATGATGATGTCTTGTCTGGTAACCTCTGGATTATCTCAAGACTAATGGCATCAAATGGGTGGAAGGAGATTGCGGTAGAACTAAGCAAGTGTCTAGGCACCAATGTCATCATCAATCCTCTGTTTGCAGACAAAGCCCTCATCAAACTAAGCCAAGGAAATATCAACGACAAAGTGCCAATTAAGGGTAAATGGACTAAGTTTGGCCTTTTTCACCTTCATCTGGAAAAATGGAATTCAAATAGTCATGGAAGGCCCTCAGTAATGCAAGGTTATAGGGGTTGGATCACAATAAAAAACCTTCCTTTAGATTATTGGTGTAGGAAAATTTTTGAAGCAATTGTGGCTTATTTTGGTGGATTAATTGCTATCGCTTCAGAAACTTTTAATCTCATCAACTGTTTAGAAGCAAAAATTCAAGTCAAGAGGAATTTCTGTGGATTTATGCCAGCCACCATTGAAGTTAAGGATGATATTCGTGGAAGTTTTTACTTGCACTTTGGTGATATTACTTGCTGTCATCCGCTTTCAAAACTACCCACTAAAGTCTTGCTTAAAGACTTCTCCAATCCAATTGACCTAGTTCGGGTACATGATGCTCTAAAGGATGAAGATGCCTCATCCCAAGTTGAAGTAATTGATTTATCCAATTGGTCTTTCCTATTGTCAAATAAATCTAAGCCTAAGTTCTCATGAAGCCCAAGTGCAAAAGCAGAAAAGTTGAATGCAATCCAAAAGACACAAAATTCTTTCTTCAATCTGGGAGTATTAAGGGAAGTTGAAAAGTCACAGACCTCAACCATCAAAACGGGAGAAATAAGGGAAGTCGAACAGTCAACTTACTTTTTTAATGAGGCTGTCAAAGTAAATCAATTAAATATGGAAGCTGAAAAGTCCCCACAGCCGCAGCCTTCTGAAGGAGTTTTCCTTAAGTCAAAGGAAGCTGAGAAACCCTTGCAGCCGCAGTCTTTTGAAAGTCCATCTGGCATATCAAAGAAAACCGAAAAGCCCCCTGATTTATTTGCTGTTGGAGATAAGACTCCTTGTGTCCCACGAATAGAAATTAATTCTTCTGCTGCCATTAAGATGAAAGAGAAAGAAGTTGAAAAGCCACCATTGCCAAAAAGAAAAAAGAAAGCAAAACTTTGTGTCCCCTATAATGAAGTGCTTCACTCAGCCTCTTCCTCGATCGAGTAGGTCATAGCCAAGCTCGAAACCAAAGCTCCTCATCAGTTTTCCTCTTCCCCTACTCTCTATCTCAATTCCTCTCCCCTGCCCTCCCCTGTTTTTCCAGACCAATCGACCTCCCTGCCTCCTCTACCCTCAAACAGCTGGTCCCTTCCTTACCTTGCTCTCCCCTGCCCTAACAAAGCACCTTCCTCCTCTGTCTCTGCTTTCCCCAAGCCTCCTTTCCCTCCCCTAAACATTAAACCTGCCCTTAAACCTAGCAAAACAAACCAGAAAAGGCTAACCAGAGCCCGCCACCCCTCCTGCCTCTTCAAAACCTTTCCTAAACACTTTATCAGAAGAAAGCACCTCAGGGAACATCCCAAAAGCCTCGGACTCACCCTCAGCTCAGCCTCTTCCCAGCCCTAAGAAGCCAAAATCCCCTCTTTTCCAAAGAAGTCTTTTAATTCCAGGTTAGATTTTTCATCTTCTCTTCCCCCTTACAACCCGACTGGACCATCACCAAAAGAAACCCCTTTTTTCCCCTTTAACTTTACTCAATTAGTGAGAGAGACTGCCCTCCTGACTCCCCCTTTAGTATCAGTTCAGAGGAAAACAAGTGGCTTAGCAATTCAGAAGACCAAAATGAGTCAAACCTGGAAGAAACATGCTTGCTTTCCTAAATACCCTGTTTCAAACGGGGGAGGAACCTTCTGCAGAGAATAAATTCCCCAACCATGCCCTTCCAGCCCTTCTTTCAACCCTTTCTGACCTCCCACCACACCTTCTGAGCTTAGTCACTGAATGCAGCTTATTATTGGACTAACCTTTGGTTGACATTGATAATTGTTCTCGAAAAGATGAAGATTGTATCCTGGAACACCAGAGGTCTCAAAGACATTAATAAAAGATTGGCCCTTAAACGATTCTTAAAGAAAATTAACCCTTCAATTGTGTTAATTCAAGAATCCAAGAAGGAAAATTTTGATGCAAGTTTCATTAAATCTTTATGGAGTTCTAAAGATGTTGATTGGGAATTCGTTGTATCTTTTGGTGCTTCGGGTGGGCTGTTAACAATGTGGGACACAAGCCTAATTTCAGTAATAGAAACCCTCAAAGGCAGTTACTCTCTCTCAATTAAATGCAACACTAATTGTAAAAATACTTGTTGGGTTACTAATGTATATGGGCCAACCGATTACAAAAAAAGAAAATTTGTGTGGGAAGAATTTCTGTCCTTGGTAGCTTATTGTGAAGACGCATGGTGTATGGGTGGCGATTTTAATATCACAAGATGGGCATGCGAAAGATTTCCCTTGGGTAGAAGCACTAGAGGCATGAGGAAGTTTAATAAGTTCATTGAGCAAGCAAAACTCATAGAAATCCGGTTGAGTAATAGGTAGTTTACATGGTCAAGGATTTCCCCCACAGTTTGCCCACTCTGCCTACATCATAGTGAGGACTTGCTTCACCTTTTCTTTACATGCCCTTACTCAAATTGGTGCTGGAATAAGCTGCTGTGTTTTTTCAATCTTCCATGGACCTTGTGCAATGATTTCAAAAGTAATGTGTTTCAGCTCCTTGCCGGCCCCATCTCCCACAAATCCATCCGTTTGTTATGGTGTAATGCTGTAAAAGCCTTATTGGCAGGCCTTTGGTTTGAAAGAAATCAAAGAATTTTCCATGACAAAGCCACTAGTTGCCAAGACCGCCTTGAAGCCGCCCGTCGTCAAGCCTCCTCCTGGTGCCTCCTCTCCGACCCTTTCCGTGCCTACAGCCTTCCAGACTTCAACCTCAATTGGGAGGCCTTTATCTCAACCCCTCCAATCGGACAGCACTAAACTTCTCCAGATTTCCAGAATTCAGCAATGCTTGTTGAACTTTTTTATATATTTCCATTATTTTCAGGCCACTGTTTTGACTTCAACCTTAGTTTTTCACTTTTGTTATCTCTTTAGGCTTTGTCCTCTTTTTGTAATCTTGTTCTCTTATCTCCCAGTTATGGGTTAGAATTGGAGATGATGAGGGTGCTAAGGGAGTGTCAACCTAGTTGAGACGTCTGAGTGCATCTCCTGATCCTTTTACAAGCACTTGTATTCTCGCTTTGCTCTTTGTATAACTCTCTTGTACTTAGAGCTTTCGCTCCTCTCTATTTATTAATACAATGAATCGTTTCCTTTTAAAAAAAAAGATGAAGATTAGATGATATTCTAAGGTTTGGTCTTTTATTTTATTTTTCTTTTTACTTTTAGTTTTATACTCAAGTAGTGAGCTTTTTAACTAGGCTGCCTAGTTTTAGTTGGGAAAGATTAGATGAGTTGCTTATCTTTTTTCTTTTAAAAAAGGTAAGCGTAAAAATAGATATGTCAAATCGCGTGTCAGATACGTATCTGGGAAGTTCTAGATGTATCGGTATCTGATACGTATTTTTTACCTCATATGAAGTATGTGCTTCACAGGTTAGAGAGATGAGCCAGGTTCCGAATGATTTCACATAATGTTAGGATTAGCCACCCAAACAATAGTAGTTCCACTCTTTAAATTAGCCTCAACAATATGCTACTCCGTACAGGATTTACCCATTTAAACAAGCCCCTTAATGTGTGCTATCCCAGGATGGAGTTCATAGGCTCAGGTAATTCAAAAATACTTCCGCGGCCAAAATTCATAGAAATCAGAAAACATTTTGACAAGTTAAAAATGCCTTTAACATATAAATACACTTGAATATTAATTTGTTTCCCTTGTGAAGGAATAAGAGTCGATATTTTGCTATTGTTAGGTACTTAAGCACCTTGGTCCACACTCCGAAAGTACCACTAGGGAAAGCCAAGTCACTTAAGTACCACATTGGTCATCCTGTTCTAACCATTGTAGGACAAAGACACATACTACCTTGGTTCCTAACAATACTCCATCCTCGGAAAGCCGACGTCTTGGCGGCTACTCCGACGGTATTTCATTTGGCTACATCCAGTCAAAACCCTCCGTCGGTTCCACTTCGGAATGTCAACATCCGACTCTGATACCATTATTAGGTACTTGCACCTTGGAGAACCACACTCCAAAAGTCAACTATTGAGGTGGGAGAGCCAAGCTACTTAAGTACCACATTGATCATCCTATTCTAGCTAATGTGGGACAAAGGTAACCCATACTCCCATGGTTCCAAAAGAGACCATCCTTGCCAATTTTTTGAATTGTGTCTATGTTATCAGTTTTCACATGGTCTGTTTATGAATGTTGAAATAGGTCATTTGAAATTGTGGCAAGCCATTGTTTATGAATTATTATTGATCCATCCATCCCAGCTCCTAACAAGTTAAAGTTTTTATAACTTGCATTTTTAGATGAAAAATAACTTTGATGAATTTCTGAAACAGATCTCGGAGAAATTATACGGGCAAAATTTGATTTCGATGTGGTTGGTCATTATGCGAGACCTGAAGTTCTTAGCTTGGTTGTGAGGGACCATCCTGCAACCCCAGTTACTTTCACATCTACATCGACAAAAGCCGAAGATTCTTGTAAAAAGTAG

mRNA sequence

ATGGCGCTTGTGCCAACCACGGCCTCCAACGATGCGAGCCAACTGATAGCGGAGGTGGACATGGGCTCCGATTCATCTGTACTCACTGTTCGAGCCACAGTCGTTCAGGCCTCCACCATCTTCTATGACACTCCCGCCACTCTAGAAAAGGCTGAGAGGTTGTTGGCTGAAGCTGCTGGATTTGGTTCCCAGCTAGTTGTATTTCCAGAAGCCTTTGTTGGTGGTTATCCCCGTGGATCAAATTTTGGTGTTTGCATTGGGAATAGAACTCCCAAGGGGAAAGAGGAATTTCGTAAATATCACGCAGCAGCGATCGATGTACCTGGTCCTGAAGTCGATCGATTGGCAGCAATGGCTGGAAAGTATAGGGTGCATTTGGTAATGGGGGTGATAGAGAGAGATGGCTACACACTGTACTGTACTATCCTATTTTTTGATCCTCAAGGTCGTTACTTGGGTAAGCACAGGAAATTGATGCCAACTGCATTGGAGCGGCTAATCTGGGGGTTTGGTGATGGATCAACAATTCCTGTTTTTGAGACTTCAATTGGAAAGATAGGTGCTGCCATTTGTTGGGAAAACAGAATGCCACTCCTAAGGACAGCTTTGTATGCAAAAGGCATTGAAATATATTGTGCACCTACCGCTGATTCTCGGGATACATGGCAAGCATCAATGACCCATATTGCTTTGGAGGGTGGATGTTTTGTTTTGTCCGCCAACCAGTTCTGCCGAAGAAAAGACTACCCTCCTCCACCAGAGTATGTATTTTCTGGCACAGAAGAGGAACTGAGCCCAGATTCTGTTGTTTGTGCTGGAGGTAGTGCCATCATATCACCTTCAGGGACAATCCTAGCTGGACCCAATTACGATGGAGAGGCACTTATATCTGCAGATCTGGATCTCGGAGAAATTATACGGGCAAAATTTGATTTCGATGTGGTTGGTCATTATGCGAGACCTGAAGTTCTTAGCTTGGTTGTGAGGGACCATCCTGCAACCCCAGTTACTTTCACATCTACATCGACAAAAGCCGAAGATTCTTGTAAAAAGTAG

Coding sequence (CDS)

ATGGCGCTTGTGCCAACCACGGCCTCCAACGATGCGAGCCAACTGATAGCGGAGGTGGACATGGGCTCCGATTCATCTGTACTCACTGTTCGAGCCACAGTCGTTCAGGCCTCCACCATCTTCTATGACACTCCCGCCACTCTAGAAAAGGCTGAGAGGTTGTTGGCTGAAGCTGCTGGATTTGGTTCCCAGCTAGTTGTATTTCCAGAAGCCTTTGTTGGTGGTTATCCCCGTGGATCAAATTTTGGTGTTTGCATTGGGAATAGAACTCCCAAGGGGAAAGAGGAATTTCGTAAATATCACGCAGCAGCGATCGATGTACCTGGTCCTGAAGTCGATCGATTGGCAGCAATGGCTGGAAAGTATAGGGTGCATTTGGTAATGGGGGTGATAGAGAGAGATGGCTACACACTGTACTGTACTATCCTATTTTTTGATCCTCAAGGTCGTTACTTGGGTAAGCACAGGAAATTGATGCCAACTGCATTGGAGCGGCTAATCTGGGGGTTTGGTGATGGATCAACAATTCCTGTTTTTGAGACTTCAATTGGAAAGATAGGTGCTGCCATTTGTTGGGAAAACAGAATGCCACTCCTAAGGACAGCTTTGTATGCAAAAGGCATTGAAATATATTGTGCACCTACCGCTGATTCTCGGGATACATGGCAAGCATCAATGACCCATATTGCTTTGGAGGGTGGATGTTTTGTTTTGTCCGCCAACCAGTTCTGCCGAAGAAAAGACTACCCTCCTCCACCAGAGTATGTATTTTCTGGCACAGAAGAGGAACTGAGCCCAGATTCTGTTGTTTGTGCTGGAGGTAGTGCCATCATATCACCTTCAGGGACAATCCTAGCTGGACCCAATTACGATGGAGAGGCACTTATATCTGCAGATCTGGATCTCGGAGAAATTATACGGGCAAAATTTGATTTCGATGTGGTTGGTCATTATGCGAGACCTGAAGTTCTTAGCTTGGTTGTGAGGGACCATCCTGCAACCCCAGTTACTTTCACATCTACATCGACAAAAGCCGAAGATTCTTGTAAAAAGTAG

Protein sequence

MALVPTTASNDASQLIAEVDMGSDSSVLTVRATVVQASTIFYDTPATLEKAERLLAEAAGFGSQLVVFPEAFVGGYPRGSNFGVCIGNRTPKGKEEFRKYHAAAIDVPGPEVDRLAAMAGKYRVHLVMGVIERDGYTLYCTILFFDPQGRYLGKHRKLMPTALERLIWGFGDGSTIPVFETSIGKIGAAICWENRMPLLRTALYAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSANQFCRRKDYPPPPEYVFSGTEEELSPDSVVCAGGSAIISPSGTILAGPNYDGEALISADLDLGEIIRAKFDFDVVGHYARPEVLSLVVRDHPATPVTFTSTSTKAEDSCKK

Homology

[Close]

BLAST of Bhi08G000022 vs. Swiss-Prot
Match: sp|Q42965|NRL4A_TOBAC (Bifunctional nitrilase/nitrile hydratase NIT4A OS=Nicotiana tabacum OX=4097 GN=NIT4A PE=2 SV=1)

HSP 1 Score: 619.4 bits (1596), Expect = 2.5e-176
Identity = 300/350 (85.71%), Postives = 324/350 (92.57%), Query Frame = 0


Query: 1   MALVPTTASNDASQLIAEVDMGSDSSVLTVRATVVQASTIFYDTPATLEKAERLLAEAAG 60
           MALVPT A N+   L AEVDMG +SS  TVRATVVQASTIFYDTPATL KAERLLAEAA 
Sbjct: 1   MALVPTPAVNE-GPLFAEVDMGDNSSTPTVRATVVQASTIFYDTPATLVKAERLLAEAAS 60

Query: 61  FGSQLVVFPEAFVGGYPRGSNFGVCIGNRTPKGKEEFRKYHAAAIDVPGPEVDRLAAMAG 120
           +G+QLVVFPEAF+GGYPRGS FGV IGNRT KGKEEFRKYHA+AIDVPGPEVDRLAAMAG
Sbjct: 61  YGAQLVVFPEAFIGGYPRGSTFGVSIGNRTAKGKEEFRKYHASAIDVPGPEVDRLAAMAG 120

Query: 121 KYRVHLVMGVIERDGYTLYCTILFFDPQGRYLGKHRKLMPTALERLIWGFGDGSTIPVFE 180
           KY+V+LVMGVIERDGYTLYCT+LFFD QG +LGKHRK+MPTALER+IWGFGDGSTIPV++
Sbjct: 121 KYKVYLVMGVIERDGYTLYCTVLFFDSQGHFLGKHRKIMPTALERIIWGFGDGSTIPVYD 180

Query: 181 TSIGKIGAAICWENRMPLLRTALYAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSA 240
           T +GKIGAAICWENRMPLLRTA+YAKGIEIYCAPTADSRD WQASMTHIALEGGCFVLSA
Sbjct: 181 TPLGKIGAAICWENRMPLLRTAMYAKGIEIYCAPTADSRDVWQASMTHIALEGGCFVLSA 240

Query: 241 NQFCRRKDYPPPPEYVFSGTEEELSPDSVVCAGGSAIISPSGTILAGPNYDGEALISADL 300
           NQFCRRKDYPPPPEYVFSGTEE+L+PDS+VCAGGS IISPSG +LAGPNY GEALISADL
Sbjct: 241 NQFCRRKDYPPPPEYVFSGTEEDLTPDSIVCAGGSVIISPSGAVLAGPNYVGEALISADL 300

Query: 301 DLGEIIRAKFDFDVVGHYARPEVLSLVVRDHPATPVTFTSTSTKAEDSCK 351
           DLGEI RAKFDFDVVGHYARPEVLSL+VRDH  +PV+FTSTS+KAE S K
Sbjct: 301 DLGEIARAKFDFDVVGHYARPEVLSLIVRDHAVSPVSFTSTSSKAESSPK 349

[Close]

BLAST of Bhi08G000022 vs. Swiss-Prot
Match: sp|Q42966|NRL4B_TOBAC (Bifunctional nitrilase/nitrile hydratase NIT4B OS=Nicotiana tabacum OX=4097 GN=NIT4B PE=2 SV=1)

HSP 1 Score: 615.5 bits (1586), Expect = 3.6e-175
Identity = 298/350 (85.14%), Postives = 324/350 (92.57%), Query Frame = 0


Query: 1   MALVPTTASNDASQLIAEVDMGSDSSVLTVRATVVQASTIFYDTPATLEKAERLLAEAAG 60
           MALVPT   N+   + AEVDMG +SS  TVRATVVQASTIFYDTPATL+KAERLLAEAA 
Sbjct: 1   MALVPTPVVNE-GPMFAEVDMGDNSSTPTVRATVVQASTIFYDTPATLDKAERLLAEAAS 60

Query: 61  FGSQLVVFPEAFVGGYPRGSNFGVCIGNRTPKGKEEFRKYHAAAIDVPGPEVDRLAAMAG 120
           +G+QLVVFPEAF+GGYPRGS FGV IGNRT KGKEEFRKYHA+AIDVPGPEVDRLAAMAG
Sbjct: 61  YGAQLVVFPEAFIGGYPRGSTFGVSIGNRTAKGKEEFRKYHASAIDVPGPEVDRLAAMAG 120

Query: 121 KYRVHLVMGVIERDGYTLYCTILFFDPQGRYLGKHRKLMPTALERLIWGFGDGSTIPVFE 180
           KY+V+LVMGVIERDGYTLYCT+LFFD QG YLGKHRK+MPTALER+IWGFGDGSTIPV++
Sbjct: 121 KYKVYLVMGVIERDGYTLYCTVLFFDSQGHYLGKHRKIMPTALERIIWGFGDGSTIPVYD 180

Query: 181 TSIGKIGAAICWENRMPLLRTALYAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSA 240
           T +GKIGAAICWENRMPLLRTA+YAKGIEIYCAPTADSRD WQASMTHIALEGGCFVLSA
Sbjct: 181 TPLGKIGAAICWENRMPLLRTAMYAKGIEIYCAPTADSRDVWQASMTHIALEGGCFVLSA 240

Query: 241 NQFCRRKDYPPPPEYVFSGTEEELSPDSVVCAGGSAIISPSGTILAGPNYDGEALISADL 300
           NQFCRRKDYPPPPEYVFSGT E+L+PDS+VCAGGS IISPSG +LAGPNY+GEALISADL
Sbjct: 241 NQFCRRKDYPPPPEYVFSGT-EDLTPDSIVCAGGSVIISPSGAVLAGPNYEGEALISADL 300

Query: 301 DLGEIIRAKFDFDVVGHYARPEVLSLVVRDHPATPVTFTSTSTKAEDSCK 351
           DLGEI RAKFDFDVVGHYARPEVLSL+VRDH  +PV+FTSTS+KAE S K
Sbjct: 301 DLGEIARAKFDFDVVGHYARPEVLSLIVRDHAVSPVSFTSTSSKAESSPK 348

[Close]

BLAST of Bhi08G000022 vs. Swiss-Prot
Match: sp|Q5QGZ8|NRL4A_LUPAN (Bifunctional nitrilase/nitrile hydratase NIT4A OS=Lupinus angustifolius OX=3871 GN=NIT4A PE=1 SV=1)

HSP 1 Score: 612.8 bits (1579), Expect = 2.3e-174
Identity = 295/350 (84.29%), Postives = 320/350 (91.43%), Query Frame = 0


Query: 1   MALVPTTASNDASQLIAEVDMGSDSSVLTVRATVVQASTIFYDTPATLEKAERLLAEAAG 60
           MALV T   ND   L AEV+M SD +  TVRATVVQASTIFYDTPATL+KAERLLAEAA 
Sbjct: 1   MALVTTPTVND-GPLFAEVNMSSDFNAPTVRATVVQASTIFYDTPATLDKAERLLAEAAS 60

Query: 61  FGSQLVVFPEAFVGGYPRGSNFGVCIGNRTPKGKEEFRKYHAAAIDVPGPEVDRLAAMAG 120
           +G+Q+VVFPEAF+GGYPRGSNFGV IGNRT KGKE+FRKYH+AAIDVPGPEVDRLAA+AG
Sbjct: 61  YGAQIVVFPEAFIGGYPRGSNFGVSIGNRTAKGKEDFRKYHSAAIDVPGPEVDRLAALAG 120

Query: 121 KYRVHLVMGVIERDGYTLYCTILFFDPQGRYLGKHRKLMPTALERLIWGFGDGSTIPVFE 180
           KY+V+LVMGVIERDGYTLYCT+LFF  QGRYLGKHRKLMPTALER+IWGFGDGSTIPVFE
Sbjct: 121 KYKVYLVMGVIERDGYTLYCTVLFFGAQGRYLGKHRKLMPTALERIIWGFGDGSTIPVFE 180

Query: 181 TSIGKIGAAICWENRMPLLRTALYAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSA 240
           T IGKIGAAICWEN+MPLLRTA+YAKG+EIYCAPTADSR+ WQASMTHIALEGGCFVLSA
Sbjct: 181 TPIGKIGAAICWENKMPLLRTAMYAKGVEIYCAPTADSREVWQASMTHIALEGGCFVLSA 240

Query: 241 NQFCRRKDYPPPPEYVFSGTEEELSPDSVVCAGGSAIISPSGTILAGPNYDGEALISADL 300
           NQFCRR+DYPPPPEYVF GTEE L+PDSVVCAGGS IISPSG +LAGP+Y+GEALISADL
Sbjct: 241 NQFCRRRDYPPPPEYVFEGTEENLTPDSVVCAGGSVIISPSGAVLAGPSYEGEALISADL 300

Query: 301 DLGEIIRAKFDFDVVGHYARPEVLSLVVRDHPATPVTFTSTSTKAEDSCK 351
           DLGEI RAKFDFDVVGHY+RPEVLSLVV+DHP  PVTFTS STK ED  K
Sbjct: 301 DLGEIARAKFDFDVVGHYSRPEVLSLVVKDHPTNPVTFTSASTKIEDKTK 349

[Close]

BLAST of Bhi08G000022 vs. Swiss-Prot
Match: sp|Q3LRV4|NRL4B_LUPAN (Bifunctional nitrilase/nitrile hydratase NIT4B OS=Lupinus angustifolius OX=3871 GN=NIT4B PE=1 SV=1)

HSP 1 Score: 610.5 bits (1573), Expect = 1.1e-173
Identity = 293/350 (83.71%), Postives = 320/350 (91.43%), Query Frame = 0


Query: 1   MALVPTTASNDASQLIAEVDMGSDSSVLTVRATVVQASTIFYDTPATLEKAERLLAEAAG 60
           MALV TT + +   L AEVDM S  +  TVRATVVQASTIFYDTPATL+KAERLL +AA 
Sbjct: 1   MALVTTTPTVNDEPLFAEVDMASYFTSTTVRATVVQASTIFYDTPATLDKAERLLVQAAS 60

Query: 61  FGSQLVVFPEAFVGGYPRGSNFGVCIGNRTPKGKEEFRKYHAAAIDVPGPEVDRLAAMAG 120
           +G+Q+VVFPEAF+GGYPRGSNFGV IGNRT KGKEEFRKYH+AAIDVPGPEVDRL+AMAG
Sbjct: 61  YGAQIVVFPEAFIGGYPRGSNFGVSIGNRTAKGKEEFRKYHSAAIDVPGPEVDRLSAMAG 120

Query: 121 KYRVHLVMGVIERDGYTLYCTILFFDPQGRYLGKHRKLMPTALERLIWGFGDGSTIPVFE 180
           KY+V+LVMGVIERDGYTLYCT+LFFD QGRYLGKHRK+MPTALER+IWGFGDGSTIPVF+
Sbjct: 121 KYKVYLVMGVIERDGYTLYCTVLFFDSQGRYLGKHRKVMPTALERIIWGFGDGSTIPVFQ 180

Query: 181 TSIGKIGAAICWENRMPLLRTALYAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSA 240
           T IGKIGAAICWEN+MPLLRTA+YAKG+EIYCAPTADSRD WQAS THIALEGGCFVLSA
Sbjct: 181 TPIGKIGAAICWENKMPLLRTAMYAKGVEIYCAPTADSRDLWQASTTHIALEGGCFVLSA 240

Query: 241 NQFCRRKDYPPPPEYVFSGTEEELSPDSVVCAGGSAIISPSGTILAGPNYDGEALISADL 300
           NQFCRRKDYPPPPEYVFSGTEE+L+PDSVV AGGS IISPSG +LAGPNY+GEALISADL
Sbjct: 241 NQFCRRKDYPPPPEYVFSGTEEDLTPDSVVSAGGSVIISPSGAVLAGPNYEGEALISADL 300

Query: 301 DLGEIIRAKFDFDVVGHYARPEVLSLVVRDHPATPVTFTSTSTKAEDSCK 351
           DLGEI RAKFDFDVVGHY+R EVLSL+V+DHP  PVTFTSTSTK ED  K
Sbjct: 301 DLGEIARAKFDFDVVGHYSRSEVLSLIVKDHPTNPVTFTSTSTKIEDQTK 350

[Close]

BLAST of Bhi08G000022 vs. Swiss-Prot
Match: sp|P46011|NRL4_ARATH (Bifunctional nitrilase/nitrile hydratase NIT4 OS=Arabidopsis thaliana OX=3702 GN=NIT4 PE=1 SV=1)

HSP 1 Score: 548.5 bits (1412), Expect = 5.4e-155
Identity = 263/348 (75.57%), Postives = 301/348 (86.49%), Query Frame = 0


Query: 1   MALVPTTASNDASQLIAEVDMGSDSSVLTVRATVVQASTIFYDTPATLEKAERLLAEAAG 60
           M   P T   +  Q+  E+DM +  S   VRATVVQAST+FYDTPATL+KAERLL+EAA 
Sbjct: 10  MTAAPQT---NGHQIFPEIDMSAGDSSSIVRATVVQASTVFYDTPATLDKAERLLSEAAE 69

Query: 61  FGSQLVVFPEAFVGGYPRGSNFGVCIGNRTPKGKEEFRKYHAAAIDVPGPEVDRLAAMAG 120
            GSQLVVFPEAF+GGYPRGS F + IG+RT KG+++FRKYHA+AIDVPGPEV+RLA MA 
Sbjct: 70  NGSQLVVFPEAFIGGYPRGSTFELAIGSRTAKGRDDFRKYHASAIDVPGPEVERLALMAK 129

Query: 121 KYRVHLVMGVIERDGYTLYCTILFFDPQGRYLGKHRKLMPTALERLIWGFGDGSTIPVFE 180
           KY+V+LVMGVIER+GYTLYCT+LFFD QG +LGKHRKLMPTALER IWGFGDGSTIPVF+
Sbjct: 130 KYKVYLVMGVIEREGYTLYCTVLFFDSQGLFLGKHRKLMPTALERCIWGFGDGSTIPVFD 189

Query: 181 TSIGKIGAAICWENRMPLLRTALYAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSA 240
           T IGKIGAAICWENRMP LRTA+YAKGIEIYCAPTADSR+TW ASMTHIALEGGCFVLSA
Sbjct: 190 TPIGKIGAAICWENRMPSLRTAMYAKGIEIYCAPTADSRETWLASMTHIALEGGCFVLSA 249

Query: 241 NQFCRRKDYPPPPEYVFSGTEEELSPDSVVCAGGSAIISPSGTILAGPNYDGEALISADL 300
           NQFCRRKDYP PPEY+FSG+EE L+PDSVVCAGGS+IISP G +LAGPNY GEALI+ADL
Sbjct: 250 NQFCRRKDYPSPPEYMFSGSEESLTPDSVVCAGGSSIISPLGIVLAGPNYRGEALITADL 309

Query: 301 DLGEIIRAKFDFDVVGHYARPEVLSLVVRDHPATPVTFTSTSTKAEDS 349
           DLG+I RAKFDFDVVGHY+RPEV SL +R+HP   V+F ++    ++S
Sbjct: 310 DLGDIARAKFDFDVVGHYSRPEVFSLNIREHPRKAVSFKTSKVMEDES 354

[Close]

BLAST of Bhi08G000022 vs. TAIR10
Match: AT5G22300.1 (nitrilase 4)

HSP 1 Score: 548.5 bits (1412), Expect = 3.0e-156
Identity = 263/348 (75.57%), Postives = 301/348 (86.49%), Query Frame = 0


Query: 1   MALVPTTASNDASQLIAEVDMGSDSSVLTVRATVVQASTIFYDTPATLEKAERLLAEAAG 60
           M   P T   +  Q+  E+DM +  S   VRATVVQAST+FYDTPATL+KAERLL+EAA 
Sbjct: 10  MTAAPQT---NGHQIFPEIDMSAGDSSSIVRATVVQASTVFYDTPATLDKAERLLSEAAE 69

Query: 61  FGSQLVVFPEAFVGGYPRGSNFGVCIGNRTPKGKEEFRKYHAAAIDVPGPEVDRLAAMAG 120
            GSQLVVFPEAF+GGYPRGS F + IG+RT KG+++FRKYHA+AIDVPGPEV+RLA MA 
Sbjct: 70  NGSQLVVFPEAFIGGYPRGSTFELAIGSRTAKGRDDFRKYHASAIDVPGPEVERLALMAK 129

Query: 121 KYRVHLVMGVIERDGYTLYCTILFFDPQGRYLGKHRKLMPTALERLIWGFGDGSTIPVFE 180
           KY+V+LVMGVIER+GYTLYCT+LFFD QG +LGKHRKLMPTALER IWGFGDGSTIPVF+
Sbjct: 130 KYKVYLVMGVIEREGYTLYCTVLFFDSQGLFLGKHRKLMPTALERCIWGFGDGSTIPVFD 189

Query: 181 TSIGKIGAAICWENRMPLLRTALYAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSA 240
           T IGKIGAAICWENRMP LRTA+YAKGIEIYCAPTADSR+TW ASMTHIALEGGCFVLSA
Sbjct: 190 TPIGKIGAAICWENRMPSLRTAMYAKGIEIYCAPTADSRETWLASMTHIALEGGCFVLSA 249

Query: 241 NQFCRRKDYPPPPEYVFSGTEEELSPDSVVCAGGSAIISPSGTILAGPNYDGEALISADL 300
           NQFCRRKDYP PPEY+FSG+EE L+PDSVVCAGGS+IISP G +LAGPNY GEALI+ADL
Sbjct: 250 NQFCRRKDYPSPPEYMFSGSEESLTPDSVVCAGGSSIISPLGIVLAGPNYRGEALITADL 309

Query: 301 DLGEIIRAKFDFDVVGHYARPEVLSLVVRDHPATPVTFTSTSTKAEDS 349
           DLG+I RAKFDFDVVGHY+RPEV SL +R+HP   V+F ++    ++S
Sbjct: 310 DLGDIARAKFDFDVVGHYSRPEVFSLNIREHPRKAVSFKTSKVMEDES 354

[Close]

BLAST of Bhi08G000022 vs. TAIR10
Match: AT3G44300.1 (nitrilase 2)

HSP 1 Score: 469.2 bits (1206), Expect = 2.3e-132
Identity = 219/327 (66.97%), Postives = 263/327 (80.43%), Query Frame = 0


Query: 25  SSVLTVRATVVQASTIFYDTPATLEKAERLLAEAAGFGSQLVVFPEAFVGGYPRGSNFGV 84
           +S   VRAT+VQAST++ DTPATLEKA + + EAA  GS+LVVFPEAF+GGYPRG  FG+
Sbjct: 13  ASSTIVRATIVQASTVYNDTPATLEKANKFIVEAASKGSELVVFPEAFIGGYPRGFRFGL 72

Query: 85  CIGNRTPKGKEEFRKYHAAAIDVPGPEVDRLAAMAGKYRVHLVMGVIERDGYTLYCTILF 144
            +G    +G++EFRKYHA+AI VPGPEV++LA +AGK  V+LVMG IE+DGYTLYCT LF
Sbjct: 73  GVGVHNEEGRDEFRKYHASAIKVPGPEVEKLAELAGKNNVYLVMGAIEKDGYTLYCTALF 132

Query: 145 FDPQGRYLGKHRKLMPTALERLIWGFGDGSTIPVFETSIGKIGAAICWENRMPLLRTALY 204
           F PQG++LGKHRKLMPT+LER IWG GDGSTIPV++T IGK+GAAICWENRMPL RTALY
Sbjct: 133 FSPQGQFLGKHRKLMPTSLERCIWGQGDGSTIPVYDTPIGKLGAAICWENRMPLYRTALY 192

Query: 205 AKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSANQFCRRKDYPPPPEYVFSGTEEEL 264
           AKGIE+YCAPTAD    WQ+SM HIA+EGGCFVLSA QFC RKD+P  P+Y+F+   ++ 
Sbjct: 193 AKGIELYCAPTADGSKEWQSSMLHIAIEGGCFVLSACQFCLRKDFPDHPDYLFTDWYDDK 252

Query: 265 SPDSVVCAGGSAIISPSGTILAGPNYDGEALISADLDLGEIIRAKFDFDVVGHYARPEVL 324
            PDS+V  GGS IISP G +LAGPN++ E LI+ADLDLG++ RAK  FD VGHY+RP+VL
Sbjct: 253 EPDSIVSQGGSVIISPLGQVLAGPNFESEGLITADLDLGDVARAKLYFDSVGHYSRPDVL 312

Query: 325 SLVVRDHPATPVTFTSTSTKAEDSCKK 352
            L V +HP  PVTF S   KAED   K
Sbjct: 313 HLTVNEHPKKPVTFISKVEKAEDDSNK 339

[Close]

BLAST of Bhi08G000022 vs. TAIR10
Match: AT3G44320.1 (nitrilase 3)

HSP 1 Score: 462.2 bits (1188), Expect = 2.8e-130
Identity = 212/323 (65.63%), Postives = 260/323 (80.50%), Query Frame = 0


Query: 29  TVRATVVQASTIFYDTPATLEKAERLLAEAAGFGSQLVVFPEAFVGGYPRGSNFGVCIGN 88
           TVR T+VQ+ST++ DTPATL+KAE+ + EAA  G++LV+FPEAF+GGYPRG  FG+ +G 
Sbjct: 24  TVRVTIVQSSTVYNDTPATLDKAEKFIVEAASKGAKLVLFPEAFIGGYPRGFRFGLAVGV 83

Query: 89  RTPKGKEEFRKYHAAAIDVPGPEVDRLAAMAGKYRVHLVMGVIERDGYTLYCTILFFDPQ 148
              +G++EFR YHA+AI VPGPEV+RLA +AGK  VHLVMG IE+DGYTLYCT LFF PQ
Sbjct: 84  HNEEGRDEFRNYHASAIKVPGPEVERLAELAGKNNVHLVMGAIEKDGYTLYCTALFFSPQ 143

Query: 149 GRYLGKHRKLMPTALERLIWGFGDGSTIPVFETSIGKIGAAICWENRMPLLRTALYAKGI 208
           G++LGKHRK+MPT+LER IWG GDGSTIPV++T IGKIGAAICWENRMPL RTALYAKGI
Sbjct: 144 GQFLGKHRKVMPTSLERCIWGQGDGSTIPVYDTPIGKIGAAICWENRMPLYRTALYAKGI 203

Query: 209 EIYCAPTADSRDTWQASMTHIALEGGCFVLSANQFCRRKDYPPPPEYVFSGTEEELSPDS 268
           EIYCAPTAD    WQASM HIA+EGGCFVLSA+QFC+R+++P  P+Y+F+   +    D 
Sbjct: 204 EIYCAPTADYSLEWQASMIHIAVEGGCFVLSAHQFCKRREFPEHPDYLFNDIVDTKEHDP 263

Query: 269 VVCAGGSAIISPSGTILAGPNYDGEALISADLDLGEIIRAKFDFDVVGHYARPEVLSLVV 328
            V  GGS IISP G +LAGPNY+ E L++ADLDLG+I RAK  FDVVGHY++P++ +L V
Sbjct: 264 TVSGGGSVIISPLGKVLAGPNYESEGLVTADLDLGDIARAKLYFDVVGHYSKPDIFNLTV 323

Query: 329 RDHPATPVTFTSTSTKAEDSCKK 352
            +HP  PVTF +   KAED   K
Sbjct: 324 NEHPKKPVTFMTKVEKAEDESNK 346

[Close]

BLAST of Bhi08G000022 vs. TAIR10
Match: AT3G44310.1 (nitrilase 1)

HSP 1 Score: 451.4 bits (1160), Expect = 4.9e-127
Identity = 207/323 (64.09%), Postives = 258/323 (79.88%), Query Frame = 0


Query: 29  TVRATVVQASTIFYDTPATLEKAERLLAEAAGFGSQLVVFPEAFVGGYPRGSNFGVCIGN 88
           TVR T+VQ+ST++ DTPAT++KAE+ + EAA  G++LV+FPE F+GGYPRG  FG+ +G 
Sbjct: 24  TVRVTIVQSSTVYNDTPATIDKAEKYIVEAASKGAELVLFPEGFIGGYPRGFRFGLAVGV 83

Query: 89  RTPKGKEEFRKYHAAAIDVPGPEVDRLAAMAGKYRVHLVMGVIERDGYTLYCTILFFDPQ 148
              +G++EFRKYHA+AI VPGPEV RLA +A K  V+LVMG IE++GYTLYCT+LFF PQ
Sbjct: 84  HNEEGRDEFRKYHASAIHVPGPEVARLADVARKNHVYLVMGAIEKEGYTLYCTVLFFSPQ 143

Query: 149 GRYLGKHRKLMPTALERLIWGFGDGSTIPVFETSIGKIGAAICWENRMPLLRTALYAKGI 208
           G++LGKHRKLMPT+LER IWG GDGSTIPV++T IGK+GAAICWENRMPL RTALYAKGI
Sbjct: 144 GQFLGKHRKLMPTSLERCIWGQGDGSTIPVYDTPIGKLGAAICWENRMPLYRTALYAKGI 203

Query: 209 EIYCAPTADSRDTWQASMTHIALEGGCFVLSANQFCRRKDYPPPPEYVFSGTEEELSPDS 268
           E+YCAPTAD    WQ+SM HIA+EGGCFVLSA QFC+RK +P  P+Y+F+   ++   DS
Sbjct: 204 ELYCAPTADGSKEWQSSMLHIAIEGGCFVLSACQFCQRKHFPDHPDYLFTDWYDDKEHDS 263

Query: 269 VVCAGGSAIISPSGTILAGPNYDGEALISADLDLGEIIRAKFDFDVVGHYARPEVLSLVV 328
           +V  GGS IISP G +LAGPN++ E L++AD+DLG+I RAK  FD VGHY+RP+VL L V
Sbjct: 264 IVSQGGSVIISPLGQVLAGPNFESEGLVTADIDLGDIARAKLYFDSVGHYSRPDVLHLTV 323

Query: 329 RDHPATPVTFTSTSTKAEDSCKK 352
            +HP   VTF +   KAED   K
Sbjct: 324 NEHPRKSVTFVTKVEKAEDDSNK 346

[Close]

BLAST of Bhi08G000022 vs. TAIR10
Match: AT2G27450.2 (nitrilase-like protein 1)

HSP 1 Score: 79.3 bits (194), Expect = 5.1e-15
Identity = 76/289 (26.30%), Postives = 120/289 (41.52%), Query Frame = 0


Query: 54  LLAEAAGFGSQLVVFPEAFVGGYPRGSNFGVCIGNRTPKGKEEFRKYHAAAIDVPGPEVD 113
           L+ EA   G+ +++  E F G Y        C   R     E+F  +  A      P + 
Sbjct: 60  LVREAHAKGANIILIQELFEGYY-------FCQAQR-----EDF--FKRAKPYKNHPTIA 119

Query: 114 RLAAMAGKYRVHLVMGVIERDGYTLYCTILFFDPQGRYLGKHRKLM----PTALERLIWG 173
           R+  +A +  V + +   E      Y +I   D  G  LG +RK      P   E+  + 
Sbjct: 120 RMQKLAKELGVVIPVSFFEEANTAHYNSIAIIDADGTDLGIYRKSHIPDGPGYQEKFYFN 179

Query: 174 FGDGSTIPVFETSIGKIGAAICWENRMPLLRTALYAKGIEIYCAPTA----------DSR 233
            GD +   VF+T   KIG AICW+   P    A+  +G EI   PTA          DSR
Sbjct: 180 PGD-TGFKVFQTKFAKIGVAICWDQWFPEAARAMVLQGAEILFYPTAIGSEPQDQGLDSR 239

Query: 234 DTWQASMTHIALEGGCFVLSANQFCRRKDYPPPPEYVFSGTEEELSPDSVVCAGGSAIIS 293
           D W+  M   A      ++++N+  +               E E  P  +   G S I  
Sbjct: 240 DHWRRVMQGHAGANVVPLVASNRIGKEI------------IETEHGPSQITFYGTSFIAG 299

Query: 294 PSGTILAGPNYDGEALISADLDLGEIIRAKFDFDVVGHYARPEVLSLVV 329
           P+G I+A  +   EA++ A  DL ++I++K     V    RP++  +++
Sbjct: 300 PTGEIVAEADDKSEAVLVAQFDL-DMIKSKRQSWGVFRDRRPDLYKVLL 320

[Close]

BLAST of Bhi08G000022 vs. TrEMBL
Match: tr|A0A1S3CLB4|A0A1S3CLB4_CUCME (bifunctional nitrilase/nitrile hydratase NIT4A OS=Cucumis melo OX=3656 GN=LOC103501743 PE=4 SV=1)

HSP 1 Score: 685.3 bits (1767), Expect = 7.4e-194
Identity = 336/351 (95.73%), Postives = 344/351 (98.01%), Query Frame = 0


Query: 1   MALVPTTASNDASQLIAEVDMGSDSSVLTVRATVVQASTIFYDTPATLEKAERLLAEAAG 60
           MALVPTTA  D  QLIAEVDMG+DSSV+TVRATVVQASTIFYDTPATL+KAERLLAEAAG
Sbjct: 1   MALVPTTAPTD-PQLIAEVDMGTDSSVMTVRATVVQASTIFYDTPATLDKAERLLAEAAG 60

Query: 61  FGSQLVVFPEAFVGGYPRGSNFGVCIGNRTPKGKEEFRKYHAAAIDVPGPEVDRLAAMAG 120
           FGSQLVVFPEAFVGGYPRGSNFGV IGNRTPKGKEEFRKYHAAAIDVPGPEVDRLAAMAG
Sbjct: 61  FGSQLVVFPEAFVGGYPRGSNFGVSIGNRTPKGKEEFRKYHAAAIDVPGPEVDRLAAMAG 120

Query: 121 KYRVHLVMGVIERDGYTLYCTILFFDPQGRYLGKHRKLMPTALERLIWGFGDGSTIPVFE 180
           KYRVHLVMGVIERDGYTLYCT+LFFDPQGRYLGKHRK+MPTALER+IWGFGDGSTIPVFE
Sbjct: 121 KYRVHLVMGVIERDGYTLYCTVLFFDPQGRYLGKHRKVMPTALERIIWGFGDGSTIPVFE 180

Query: 181 TSIGKIGAAICWENRMPLLRTALYAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSA 240
           TSIGKIGAAICWENRMPLLRTA+YAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSA
Sbjct: 181 TSIGKIGAAICWENRMPLLRTAMYAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSA 240

Query: 241 NQFCRRKDYPPPPEYVFSGTEEELSPDSVVCAGGSAIISPSGTILAGPNYDGEALISADL 300
           NQFCRRKDYPPPPEYVFSGTEEEL+PDSVVCAGGSAIISPSGTILAGPNYDGEALISADL
Sbjct: 241 NQFCRRKDYPPPPEYVFSGTEEELTPDSVVCAGGSAIISPSGTILAGPNYDGEALISADL 300

Query: 301 DLGEIIRAKFDFDVVGHYARPEVLSLVVRDHPATPVTFTSTSTKAEDSCKK 352
           DLGEI RAKFDFDVVGHYARPEVLSLVVRDHPA PVTFTSTSTKAEDSCKK
Sbjct: 301 DLGEIARAKFDFDVVGHYARPEVLSLVVRDHPAIPVTFTSTSTKAEDSCKK 350

[Close]

BLAST of Bhi08G000022 vs. TrEMBL
Match: tr|A0A0A0LVI0|A0A0A0LVI0_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G168360 PE=4 SV=1)

HSP 1 Score: 684.5 bits (1765), Expect = 1.3e-193
Identity = 335/351 (95.44%), Postives = 343/351 (97.72%), Query Frame = 0


Query: 1   MALVPTTASNDASQLIAEVDMGSDSSVLTVRATVVQASTIFYDTPATLEKAERLLAEAAG 60
           MALVPTTA  D SQLIAEVDMGSDSSV+ VRATVVQASTIFYDTPATL+KAERLLAEAAG
Sbjct: 1   MALVPTTAPTD-SQLIAEVDMGSDSSVMNVRATVVQASTIFYDTPATLDKAERLLAEAAG 60

Query: 61  FGSQLVVFPEAFVGGYPRGSNFGVCIGNRTPKGKEEFRKYHAAAIDVPGPEVDRLAAMAG 120
           FGSQLVVFPEAFVGGYPRGSNFGV IGNRTPKGKEEFRKYHA+AIDVPGPEVDRLAAMAG
Sbjct: 61  FGSQLVVFPEAFVGGYPRGSNFGVSIGNRTPKGKEEFRKYHASAIDVPGPEVDRLAAMAG 120

Query: 121 KYRVHLVMGVIERDGYTLYCTILFFDPQGRYLGKHRKLMPTALERLIWGFGDGSTIPVFE 180
           KYRVHLVMGVIERDGYTLYCT+LFFDPQGRYLGKHRK+MPTALER+IWGFGDGSTIPVFE
Sbjct: 121 KYRVHLVMGVIERDGYTLYCTVLFFDPQGRYLGKHRKVMPTALERIIWGFGDGSTIPVFE 180

Query: 181 TSIGKIGAAICWENRMPLLRTALYAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSA 240
           TSIGKIGAAICWENRMPLLRTA+YAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSA
Sbjct: 181 TSIGKIGAAICWENRMPLLRTAMYAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSA 240

Query: 241 NQFCRRKDYPPPPEYVFSGTEEELSPDSVVCAGGSAIISPSGTILAGPNYDGEALISADL 300
           NQFCRRKDYPPPPEYVFSGTEEEL+PDSVVCAGGSAIISPSGTILAGPNYDGEALISADL
Sbjct: 241 NQFCRRKDYPPPPEYVFSGTEEELTPDSVVCAGGSAIISPSGTILAGPNYDGEALISADL 300

Query: 301 DLGEIIRAKFDFDVVGHYARPEVLSLVVRDHPATPVTFTSTSTKAEDSCKK 352
           DLGEI RAKFDFDVVGHYARPEVLSLVVRDHP TPVTFTSTSTK EDSCKK
Sbjct: 301 DLGEIARAKFDFDVVGHYARPEVLSLVVRDHPTTPVTFTSTSTKVEDSCKK 350

[Close]

BLAST of Bhi08G000022 vs. TrEMBL
Match: tr|A0A2P5CW35|A0A2P5CW35_9ROSA (Nitrilase/cyanide hydratase OS=Trema orientalis OX=63057 GN=TorNIT1 PE=4 SV=1)

HSP 1 Score: 634.0 bits (1634), Expect = 2.0e-178
Identity = 306/348 (87.93%), Postives = 327/348 (93.97%), Query Frame = 0


Query: 1   MALVPTTASNDASQLIAEVDMGSDSSVLTVRATVVQASTIFYDTPATLEKAERLLAEAAG 60
           MALVP+   NDA  L AEVDMG+DSS  +VRATVVQAST+FYDTPATL+KAERLLAEAAG
Sbjct: 1   MALVPSAPLNDA-PLFAEVDMGADSSAPSVRATVVQASTVFYDTPATLDKAERLLAEAAG 60

Query: 61  FGSQLVVFPEAFVGGYPRGSNFGVCIGNRTPKGKEEFRKYHAAAIDVPGPEVDRLAAMAG 120
           +GSQLVVFPEAFVGGYPRGSNFGV IGNRTPKGKEEFRKYHA+AIDVPGPEVDRLAAMAG
Sbjct: 61  YGSQLVVFPEAFVGGYPRGSNFGVTIGNRTPKGKEEFRKYHASAIDVPGPEVDRLAAMAG 120

Query: 121 KYRVHLVMGVIERDGYTLYCTILFFDPQGRYLGKHRKLMPTALERLIWGFGDGSTIPVFE 180
           KY+V+LVMGVIERDGYTLYCT+LFFD QGRYLGKHRK+MPTALER+IWGFGDGSTIPVFE
Sbjct: 121 KYKVYLVMGVIERDGYTLYCTVLFFDSQGRYLGKHRKVMPTALERIIWGFGDGSTIPVFE 180

Query: 181 TSIGKIGAAICWENRMPLLRTALYAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSA 240
           T IGKIGAAICWENRMPLLRTA+YAKG+EIYCAPTAD+R+ WQASMTHIALEG CFVLSA
Sbjct: 181 TPIGKIGAAICWENRMPLLRTAMYAKGVEIYCAPTADAREVWQASMTHIALEGACFVLSA 240

Query: 241 NQFCRRKDYPPPPEYVFSGTEEELSPDSVVCAGGSAIISPSGTILAGPNYDGEALISADL 300
           NQFCRRKDYPPPPEYVFSGTE EL+PDSVVCAGGS IISPSGT+LAGPNYDGEALISADL
Sbjct: 241 NQFCRRKDYPPPPEYVFSGTEGELTPDSVVCAGGSVIISPSGTVLAGPNYDGEALISADL 300

Query: 301 DLGEIIRAKFDFDVVGHYARPEVLSLVVRDHPATPVTFTSTSTKAEDS 349
           DLGEI RAKFDFDVVGHY+RPEVLSL+VRDHP  PVTFTS+S K E S
Sbjct: 301 DLGEIARAKFDFDVVGHYSRPEVLSLIVRDHPTNPVTFTSSSLKTESS 347

[Close]

BLAST of Bhi08G000022 vs. TrEMBL
Match: tr|A0A2P5E021|A0A2P5E021_PARAD (Nitrilase/cyanide hydratase OS=Parasponia andersonii OX=3476 GN=PanNIT1 PE=4 SV=1)

HSP 1 Score: 632.5 bits (1630), Expect = 5.7e-178
Identity = 304/348 (87.36%), Postives = 327/348 (93.97%), Query Frame = 0


Query: 1   MALVPTTASNDASQLIAEVDMGSDSSVLTVRATVVQASTIFYDTPATLEKAERLLAEAAG 60
           MALVP+   NDA  L AEVDMG+DSS  TVRATVVQAST++YDTPATL+KAERLLAEAAG
Sbjct: 1   MALVPSAPLNDA-PLFAEVDMGADSSASTVRATVVQASTVYYDTPATLDKAERLLAEAAG 60

Query: 61  FGSQLVVFPEAFVGGYPRGSNFGVCIGNRTPKGKEEFRKYHAAAIDVPGPEVDRLAAMAG 120
           +GSQLVVFPEAFVGGYPRGSNFGV IGNRTPKGKEEFRKYHA+AIDVPGPEVD LAAMAG
Sbjct: 61  YGSQLVVFPEAFVGGYPRGSNFGVTIGNRTPKGKEEFRKYHASAIDVPGPEVDLLAAMAG 120

Query: 121 KYRVHLVMGVIERDGYTLYCTILFFDPQGRYLGKHRKLMPTALERLIWGFGDGSTIPVFE 180
           KY+V+LVMGVIERDGYTLYCT+LFFD QGRY+GKHRK+MPTALER+IWGFGDGSTIPVFE
Sbjct: 121 KYKVYLVMGVIERDGYTLYCTVLFFDSQGRYMGKHRKVMPTALERIIWGFGDGSTIPVFE 180

Query: 181 TSIGKIGAAICWENRMPLLRTALYAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSA 240
           T IGKIGAAICWENRMPLLRTA+YAKG+EIYCAPTAD+R+ WQASMTHIA+EGGCFVLSA
Sbjct: 181 TPIGKIGAAICWENRMPLLRTAMYAKGVEIYCAPTADAREVWQASMTHIAVEGGCFVLSA 240

Query: 241 NQFCRRKDYPPPPEYVFSGTEEELSPDSVVCAGGSAIISPSGTILAGPNYDGEALISADL 300
           NQFCRRKDYPPPPEYVFSGTE EL+PDSVVCAGGS IISPSGT+LAGPNYDGEALISADL
Sbjct: 241 NQFCRRKDYPPPPEYVFSGTEGELTPDSVVCAGGSVIISPSGTVLAGPNYDGEALISADL 300

Query: 301 DLGEIIRAKFDFDVVGHYARPEVLSLVVRDHPATPVTFTSTSTKAEDS 349
           DLGEI RAKFDFDVVGHY+RPEVLSL+VRDHP  PVTFTS+S K E S
Sbjct: 301 DLGEIARAKFDFDVVGHYSRPEVLSLIVRDHPTNPVTFTSSSLKTESS 347

[Close]

BLAST of Bhi08G000022 vs. TrEMBL
Match: tr|A0A1Q3BL52|A0A1Q3BL52_CEPFO (CN_hydrolase domain-containing protein OS=Cephalotus follicularis OX=3775 GN=CFOL_v3_12234 PE=4 SV=1)

HSP 1 Score: 629.4 bits (1622), Expect = 4.8e-177
Identity = 304/348 (87.36%), Postives = 327/348 (93.97%), Query Frame = 0


Query: 1   MALVPTTASNDASQLIAEVDMGSDSSVLTVRATVVQASTIFYDTPATLEKAERLLAEAAG 60
           MALV +   NDA+ LIAE+DMGSDSS LTVRATVVQASTIFYDTPATL+KAERLL EAAG
Sbjct: 1   MALVQSPPMNDAA-LIAEIDMGSDSSALTVRATVVQASTIFYDTPATLDKAERLLGEAAG 60

Query: 61  FGSQLVVFPEAFVGGYPRGSNFGVCIGNRTPKGKEEFRKYHAAAIDVPGPEVDRLAAMAG 120
           +GSQLVVFPEAF+GGYPRGSNFGV IGNRT KGKEEFRKYHA+AIDVPGPEVDRLAAMAG
Sbjct: 61  YGSQLVVFPEAFIGGYPRGSNFGVTIGNRTAKGKEEFRKYHASAIDVPGPEVDRLAAMAG 120

Query: 121 KYRVHLVMGVIERDGYTLYCTILFFDPQGRYLGKHRKLMPTALERLIWGFGDGSTIPVFE 180
           KY+V LVMGVIERDGYTLYCT+LFFD QG YLGKHRK+MPTALER+IWGFGDGSTIPVFE
Sbjct: 121 KYKVFLVMGVIERDGYTLYCTVLFFDSQGHYLGKHRKIMPTALERVIWGFGDGSTIPVFE 180

Query: 181 TSIGKIGAAICWENRMPLLRTALYAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSA 240
           T+IGKIGAAICWENRMPLLRTA+YAKGIEIYCAPTADSRD WQASMTHIALEGGCFVLSA
Sbjct: 181 TAIGKIGAAICWENRMPLLRTAMYAKGIEIYCAPTADSRDVWQASMTHIALEGGCFVLSA 240

Query: 241 NQFCRRKDYPPPPEYVFSGTEEELSPDSVVCAGGSAIISPSGTILAGPNYDGEALISADL 300
           NQFCRRKDYPPPPEY+FSGTE+ L+ DSVVCAGGS IISPSGT+LAGPNY+GEALISADL
Sbjct: 241 NQFCRRKDYPPPPEYIFSGTEDVLTADSVVCAGGSVIISPSGTVLAGPNYEGEALISADL 300

Query: 301 DLGEIIRAKFDFDVVGHYARPEVLSLVVRDHPATPVTFTSTSTKAEDS 349
           D+GEI RAKFDFDVVGHY+RPEVLSL+V+DHPA PVTFTS S+K E S
Sbjct: 301 DVGEIARAKFDFDVVGHYSRPEVLSLIVKDHPANPVTFTSLSSKTEGS 347

[Close]

BLAST of Bhi08G000022 vs. NCBI nr
Match: XP_008463648.1 (PREDICTED: bifunctional nitrilase/nitrile hydratase NIT4A [Cucumis melo])

HSP 1 Score: 685.3 bits (1767), Expect = 1.1e-193
Identity = 336/351 (95.73%), Postives = 344/351 (98.01%), Query Frame = 0


Query: 1   MALVPTTASNDASQLIAEVDMGSDSSVLTVRATVVQASTIFYDTPATLEKAERLLAEAAG 60
           MALVPTTA  D  QLIAEVDMG+DSSV+TVRATVVQASTIFYDTPATL+KAERLLAEAAG
Sbjct: 1   MALVPTTAPTD-PQLIAEVDMGTDSSVMTVRATVVQASTIFYDTPATLDKAERLLAEAAG 60

Query: 61  FGSQLVVFPEAFVGGYPRGSNFGVCIGNRTPKGKEEFRKYHAAAIDVPGPEVDRLAAMAG 120
           FGSQLVVFPEAFVGGYPRGSNFGV IGNRTPKGKEEFRKYHAAAIDVPGPEVDRLAAMAG
Sbjct: 61  FGSQLVVFPEAFVGGYPRGSNFGVSIGNRTPKGKEEFRKYHAAAIDVPGPEVDRLAAMAG 120

Query: 121 KYRVHLVMGVIERDGYTLYCTILFFDPQGRYLGKHRKLMPTALERLIWGFGDGSTIPVFE 180
           KYRVHLVMGVIERDGYTLYCT+LFFDPQGRYLGKHRK+MPTALER+IWGFGDGSTIPVFE
Sbjct: 121 KYRVHLVMGVIERDGYTLYCTVLFFDPQGRYLGKHRKVMPTALERIIWGFGDGSTIPVFE 180

Query: 181 TSIGKIGAAICWENRMPLLRTALYAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSA 240
           TSIGKIGAAICWENRMPLLRTA+YAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSA
Sbjct: 181 TSIGKIGAAICWENRMPLLRTAMYAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSA 240

Query: 241 NQFCRRKDYPPPPEYVFSGTEEELSPDSVVCAGGSAIISPSGTILAGPNYDGEALISADL 300
           NQFCRRKDYPPPPEYVFSGTEEEL+PDSVVCAGGSAIISPSGTILAGPNYDGEALISADL
Sbjct: 241 NQFCRRKDYPPPPEYVFSGTEEELTPDSVVCAGGSAIISPSGTILAGPNYDGEALISADL 300

Query: 301 DLGEIIRAKFDFDVVGHYARPEVLSLVVRDHPATPVTFTSTSTKAEDSCKK 352
           DLGEI RAKFDFDVVGHYARPEVLSLVVRDHPA PVTFTSTSTKAEDSCKK
Sbjct: 301 DLGEIARAKFDFDVVGHYARPEVLSLVVRDHPAIPVTFTSTSTKAEDSCKK 350

[Close]

BLAST of Bhi08G000022 vs. NCBI nr
Match: XP_004139500.1 (PREDICTED: bifunctional nitrilase/nitrile hydratase NIT4A [Cucumis sativus] >KGN64959.1 hypothetical protein Csa_1G168360 [Cucumis sativus])

HSP 1 Score: 684.5 bits (1765), Expect = 1.9e-193
Identity = 335/351 (95.44%), Postives = 343/351 (97.72%), Query Frame = 0


Query: 1   MALVPTTASNDASQLIAEVDMGSDSSVLTVRATVVQASTIFYDTPATLEKAERLLAEAAG 60
           MALVPTTA  D SQLIAEVDMGSDSSV+ VRATVVQASTIFYDTPATL+KAERLLAEAAG
Sbjct: 1   MALVPTTAPTD-SQLIAEVDMGSDSSVMNVRATVVQASTIFYDTPATLDKAERLLAEAAG 60

Query: 61  FGSQLVVFPEAFVGGYPRGSNFGVCIGNRTPKGKEEFRKYHAAAIDVPGPEVDRLAAMAG 120
           FGSQLVVFPEAFVGGYPRGSNFGV IGNRTPKGKEEFRKYHA+AIDVPGPEVDRLAAMAG
Sbjct: 61  FGSQLVVFPEAFVGGYPRGSNFGVSIGNRTPKGKEEFRKYHASAIDVPGPEVDRLAAMAG 120

Query: 121 KYRVHLVMGVIERDGYTLYCTILFFDPQGRYLGKHRKLMPTALERLIWGFGDGSTIPVFE 180
           KYRVHLVMGVIERDGYTLYCT+LFFDPQGRYLGKHRK+MPTALER+IWGFGDGSTIPVFE
Sbjct: 121 KYRVHLVMGVIERDGYTLYCTVLFFDPQGRYLGKHRKVMPTALERIIWGFGDGSTIPVFE 180

Query: 181 TSIGKIGAAICWENRMPLLRTALYAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSA 240
           TSIGKIGAAICWENRMPLLRTA+YAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSA
Sbjct: 181 TSIGKIGAAICWENRMPLLRTAMYAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSA 240

Query: 241 NQFCRRKDYPPPPEYVFSGTEEELSPDSVVCAGGSAIISPSGTILAGPNYDGEALISADL 300
           NQFCRRKDYPPPPEYVFSGTEEEL+PDSVVCAGGSAIISPSGTILAGPNYDGEALISADL
Sbjct: 241 NQFCRRKDYPPPPEYVFSGTEEELTPDSVVCAGGSAIISPSGTILAGPNYDGEALISADL 300

Query: 301 DLGEIIRAKFDFDVVGHYARPEVLSLVVRDHPATPVTFTSTSTKAEDSCKK 352
           DLGEI RAKFDFDVVGHYARPEVLSLVVRDHP TPVTFTSTSTK EDSCKK
Sbjct: 301 DLGEIARAKFDFDVVGHYARPEVLSLVVRDHPTTPVTFTSTSTKVEDSCKK 350

[Close]

BLAST of Bhi08G000022 vs. NCBI nr
Match: XP_023520715.1 (bifunctional nitrilase/nitrile hydratase NIT4A [Cucurbita pepo subsp. pepo])

HSP 1 Score: 678.3 bits (1749), Expect = 1.4e-191
Identity = 332/351 (94.59%), Postives = 341/351 (97.15%), Query Frame = 0


Query: 1   MALVPTTASNDASQLIAEVDMGSDSSVLTVRATVVQASTIFYDTPATLEKAERLLAEAAG 60
           MALVPTTA+ D +QLIAEVDMGSD SV TVRATVVQASTIFYDTPATL+KAERLLAEAAG
Sbjct: 1   MALVPTTAATD-TQLIAEVDMGSDVSVPTVRATVVQASTIFYDTPATLDKAERLLAEAAG 60

Query: 61  FGSQLVVFPEAFVGGYPRGSNFGVCIGNRTPKGKEEFRKYHAAAIDVPGPEVDRLAAMAG 120
           FGSQLVVFPEAFVGGYPRGSNFGV IGNRTPKGKEEFRKYHAAAIDVPGPEVDRLAAMAG
Sbjct: 61  FGSQLVVFPEAFVGGYPRGSNFGVSIGNRTPKGKEEFRKYHAAAIDVPGPEVDRLAAMAG 120

Query: 121 KYRVHLVMGVIERDGYTLYCTILFFDPQGRYLGKHRKLMPTALERLIWGFGDGSTIPVFE 180
           KYRVHLVMGVIERDGYTLYCT+LFFDPQGRYLGKHRK+MPTALER+IWGFGDGSTIPVFE
Sbjct: 121 KYRVHLVMGVIERDGYTLYCTVLFFDPQGRYLGKHRKVMPTALERIIWGFGDGSTIPVFE 180

Query: 181 TSIGKIGAAICWENRMPLLRTALYAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSA 240
           TSIGKIGAAICWENRMPLLRTA+YAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSA
Sbjct: 181 TSIGKIGAAICWENRMPLLRTAMYAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSA 240

Query: 241 NQFCRRKDYPPPPEYVFSGTEEELSPDSVVCAGGSAIISPSGTILAGPNYDGEALISADL 300
           NQFCRRKDYPPPP+YVFSGTEEELSPDSVVCAGGS IISPSGTILAGPNYDGEALISADL
Sbjct: 241 NQFCRRKDYPPPPDYVFSGTEEELSPDSVVCAGGSVIISPSGTILAGPNYDGEALISADL 300

Query: 301 DLGEIIRAKFDFDVVGHYARPEVLSLVVRDHPATPVTFTSTSTKAEDSCKK 352
           DLG+I RAKFDFDVVGHYARPEVLSLVVRDHP TPVTFTS STK EDSCKK
Sbjct: 301 DLGDIARAKFDFDVVGHYARPEVLSLVVRDHPTTPVTFTSASTKTEDSCKK 350

[Close]

BLAST of Bhi08G000022 vs. NCBI nr
Match: XP_022973146.1 (bifunctional nitrilase/nitrile hydratase NIT4A [Cucurbita maxima])

HSP 1 Score: 676.4 bits (1744), Expect = 5.2e-191
Identity = 331/351 (94.30%), Postives = 340/351 (96.87%), Query Frame = 0


Query: 1   MALVPTTASNDASQLIAEVDMGSDSSVLTVRATVVQASTIFYDTPATLEKAERLLAEAAG 60
           MALVPTTA+ND +QLIAEVDMGSD SV TVRATVVQASTIFYDTPATL+KAERLLAEAAG
Sbjct: 1   MALVPTTAAND-TQLIAEVDMGSDVSVPTVRATVVQASTIFYDTPATLDKAERLLAEAAG 60

Query: 61  FGSQLVVFPEAFVGGYPRGSNFGVCIGNRTPKGKEEFRKYHAAAIDVPGPEVDRLAAMAG 120
           FGSQLVVFPEAFVGGYPRGSNFGV IGNRTPKGKEEFRKYHAAAIDVPGPEVDRLAAMAG
Sbjct: 61  FGSQLVVFPEAFVGGYPRGSNFGVSIGNRTPKGKEEFRKYHAAAIDVPGPEVDRLAAMAG 120

Query: 121 KYRVHLVMGVIERDGYTLYCTILFFDPQGRYLGKHRKLMPTALERLIWGFGDGSTIPVFE 180
           KYRVHLVMGVIERDGYTLYCT+LFFDPQGRYLGKHRK+MPTALER+IWGFGDGSTIPVFE
Sbjct: 121 KYRVHLVMGVIERDGYTLYCTVLFFDPQGRYLGKHRKVMPTALERIIWGFGDGSTIPVFE 180

Query: 181 TSIGKIGAAICWENRMPLLRTALYAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSA 240
           TSIGKIGAAICWENRMPLLRTA+YAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSA
Sbjct: 181 TSIGKIGAAICWENRMPLLRTAMYAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSA 240

Query: 241 NQFCRRKDYPPPPEYVFSGTEEELSPDSVVCAGGSAIISPSGTILAGPNYDGEALISADL 300
           NQFCRRKDYPPPP+YVFSGTEEELSPDSVVCAGGS IISPSGTILAGPNYDGEALISADL
Sbjct: 241 NQFCRRKDYPPPPDYVFSGTEEELSPDSVVCAGGSVIISPSGTILAGPNYDGEALISADL 300

Query: 301 DLGEIIRAKFDFDVVGHYARPEVLSLVVRDHPATPVTFTSTSTKAEDSCKK 352
           DLG+I RAKFDFDVVGHYARPEVLSLVVRDHP TPVTFTS S K EDSC K
Sbjct: 301 DLGDIARAKFDFDVVGHYARPEVLSLVVRDHPTTPVTFTSASKKTEDSCNK 350

[Close]

BLAST of Bhi08G000022 vs. NCBI nr
Match: XP_022927562.1 (bifunctional nitrilase/nitrile hydratase NIT4A [Cucurbita moschata])

HSP 1 Score: 672.5 bits (1734), Expect = 7.5e-190
Identity = 329/350 (94.00%), Postives = 339/350 (96.86%), Query Frame = 0


Query: 1   MALVPTTASNDASQLIAEVDMGSDSSVLTVRATVVQASTIFYDTPATLEKAERLLAEAAG 60
           MALVPTTA+ D +QLIAEVDMGSD SV TVRATVVQASTIFYDTPATL+KAERLLAEAAG
Sbjct: 1   MALVPTTAAAD-TQLIAEVDMGSDVSVPTVRATVVQASTIFYDTPATLDKAERLLAEAAG 60

Query: 61  FGSQLVVFPEAFVGGYPRGSNFGVCIGNRTPKGKEEFRKYHAAAIDVPGPEVDRLAAMAG 120
           FGSQLVVFPEAFVGGYPRGSNFGV IGNRTPKGKEEFRKYHAAAIDVPGPEVDRLAAMAG
Sbjct: 61  FGSQLVVFPEAFVGGYPRGSNFGVSIGNRTPKGKEEFRKYHAAAIDVPGPEVDRLAAMAG 120

Query: 121 KYRVHLVMGVIERDGYTLYCTILFFDPQGRYLGKHRKLMPTALERLIWGFGDGSTIPVFE 180
           KYRVHLVMGVIERDGYTLYCT+LFFDPQGRYLGKHRK+MPTALER+IWGFGDGSTIPVFE
Sbjct: 121 KYRVHLVMGVIERDGYTLYCTVLFFDPQGRYLGKHRKVMPTALERIIWGFGDGSTIPVFE 180

Query: 181 TSIGKIGAAICWENRMPLLRTALYAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSA 240
           TSIGKIGAAICWENRMPLLRTA+YAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSA
Sbjct: 181 TSIGKIGAAICWENRMPLLRTAMYAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSA 240

Query: 241 NQFCRRKDYPPPPEYVFSGTEEELSPDSVVCAGGSAIISPSGTILAGPNYDGEALISADL 300
           NQFCRRKDYPPPP+YVFSGTEEELSPDSVVCAGGS IISPSGTILAGPNYDGEALISADL
Sbjct: 241 NQFCRRKDYPPPPDYVFSGTEEELSPDSVVCAGGSVIISPSGTILAGPNYDGEALISADL 300

Query: 301 DLGEIIRAKFDFDVVGHYARPEVLSLVVRDHPATPVTFTSTSTKAEDSCK 351
           DLG+I RAKFDFDVVGHYARPEVLSLVVRDHP TPVTFTS + K EDSCK
Sbjct: 301 DLGDIARAKFDFDVVGHYARPEVLSLVVRDHPTTPVTFTSATAKTEDSCK 349

The following BLAST results are available for this feature:

BLAST of Bhi08G000022 vs. Swiss-Prot


Match Name	E-value	Identity	Description
sp\|Q42965\|NRL4A_TOBAC	2.5e-176	85.71	Bifunctional nitrilase/nitrile hydratase NIT4A OS=Nicotiana tabacum OX=4097 GN=N...	[more]
sp\|Q42966\|NRL4B_TOBAC	3.6e-175	85.14	Bifunctional nitrilase/nitrile hydratase NIT4B OS=Nicotiana tabacum OX=4097 GN=N...	[more]
sp\|Q5QGZ8\|NRL4A_LUPAN	2.3e-174	84.29	Bifunctional nitrilase/nitrile hydratase NIT4A OS=Lupinus angustifolius OX=3871 ...	[more]
sp\|Q3LRV4\|NRL4B_LUPAN	1.1e-173	83.71	Bifunctional nitrilase/nitrile hydratase NIT4B OS=Lupinus angustifolius OX=3871 ...	[more]
sp\|P46011\|NRL4_ARATH	5.4e-155	75.57	Bifunctional nitrilase/nitrile hydratase NIT4 OS=Arabidopsis thaliana OX=3702 GN...	[more]

BLAST of Bhi08G000022 vs. TAIR10


Match Name	E-value	Identity	Description
AT5G22300.1	3.0e-156	75.57	nitrilase 4	[more]
AT3G44300.1	2.3e-132	66.97	nitrilase 2	[more]
AT3G44320.1	2.8e-130	65.63	nitrilase 3	[more]
AT3G44310.1	4.9e-127	64.09	nitrilase 1	[more]
AT2G27450.2	5.1e-15	26.30	nitrilase-like protein 1	[more]

BLAST of Bhi08G000022 vs. TrEMBL


Match Name	E-value	Identity	Description
tr\|A0A1S3CLB4\|A0A1S3CLB4_CUCME	7.4e-194	95.73	bifunctional nitrilase/nitrile hydratase NIT4A OS=Cucumis melo OX=3656 GN=LOC103...	[more]
tr\|A0A0A0LVI0\|A0A0A0LVI0_CUCSA	1.3e-193	95.44	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G168360 PE=4 SV=1	[more]
tr\|A0A2P5CW35\|A0A2P5CW35_9ROSA	2.0e-178	87.93	Nitrilase/cyanide hydratase OS=Trema orientalis OX=63057 GN=TorNIT1 PE=4 SV=1	[more]
tr\|A0A2P5E021\|A0A2P5E021_PARAD	5.7e-178	87.36	Nitrilase/cyanide hydratase OS=Parasponia andersonii OX=3476 GN=PanNIT1 PE=4 SV=...	[more]
tr\|A0A1Q3BL52\|A0A1Q3BL52_CEPFO	4.8e-177	87.36	CN_hydrolase domain-containing protein OS=Cephalotus follicularis OX=3775 GN=CFO...	[more]

BLAST of Bhi08G000022 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_008463648.1	1.1e-193	95.73	PREDICTED: bifunctional nitrilase/nitrile hydratase NIT4A [Cucumis melo]	[more]
XP_004139500.1	1.9e-193	95.44	PREDICTED: bifunctional nitrilase/nitrile hydratase NIT4A [Cucumis sativus] >KGN...	[more]
XP_023520715.1	1.4e-191	94.59	bifunctional nitrilase/nitrile hydratase NIT4A [Cucurbita pepo subsp. pepo]	[more]
XP_022973146.1	5.2e-191	94.30	bifunctional nitrilase/nitrile hydratase NIT4A [Cucurbita maxima]	[more]
XP_022927562.1	7.5e-190	94.00	bifunctional nitrilase/nitrile hydratase NIT4A [Cucurbita moschata]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **Molecular Function**
Term	Definition
GO:0016810	hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds

Vocabulary: **Biological Process**
Term	Definition
GO:0006807	nitrogen compound metabolic process

Vocabulary: **INTERPRO**
Term	Definition
IPR000132	Nitrilase/CN_hydratase_CS
IPR036526	C-N_Hydrolase_sf
IPR003010	C-N_Hydrolase

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0019499	cyanide metabolic process
biological_process	GO:0050898	nitrile metabolic process
biological_process	GO:0019500	cyanide catabolic process
biological_process	GO:0051410	detoxification of nitrogen compound
biological_process	GO:0006807	nitrogen compound metabolic process
cellular_component	GO:0005886	plasma membrane
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0080109	indole-3-acetonitrile nitrile hydratase activity
molecular_function	GO:0000257	nitrilase activity
molecular_function	GO:0047558	3-cyanoalanine hydratase activity
molecular_function	GO:0047427	cyanoalanine nitrilase activity
molecular_function	GO:0016810	hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Bhi08M000022	Bhi08M000022	mRNA

InterPro

Analysis Name: InterPro Annotations of wax gourd
Date Performed: 2019-11-17


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR003010	Carbon-nitrogen hydrolase	PFAM	PF00795	CN_hydrolase	coord: 31..309 e-value: 3.0E-69 score: 233.2
IPR003010	Carbon-nitrogen hydrolase	PROSITE	PS50263	CN_HYDROLASE	coord: 30..302 score: 58.449
IPR036526	Carbon-nitrogen hydrolase superfamily	GENE3D	G3DSA:3.60.110.10		coord: 22..314 e-value: 3.6E-105 score: 353.0
IPR036526	Carbon-nitrogen hydrolase superfamily	SUPERFAMILY	SSF56317	Carbon-nitrogen hydrolase	coord: 29..328
None	No IPR available	PANTHER	PTHR23088:SF31	SUBFAMILY NOT NAMED	coord: 16..345
None	No IPR available	PANTHER	PTHR23088	NITRILASE-RELATED	coord: 16..345
None	No IPR available	CDD	cd07564	nitrilases_CHs	coord: 30..330 e-value: 3.95057E-162 score: 457.72
IPR000132	Nitrilase/cyanide hydratase, conserved site	PROSITE	PS00920	NITRIL_CHT_1	coord: 62..77
IPR000132	Nitrilase/cyanide hydratase, conserved site	PROSITE	PS00921	NITRIL_CHT_2	coord: 187..200

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Bhi08G000022	Csa1G168360	Cucumber (Chinese Long) v2	cuwgoB049
Bhi08G000022	ClCG01G011750	Watermelon (Charleston Gray)	wcgwgoB232
Bhi08G000022	CSPI01G15000	Wild cucumber (PI 183967)	cpiwgoB049
Bhi08G000022	Cucsa.319690	Cucumber (Gy14) v1	cgywgoB639
Bhi08G000022	CmaCh13G003490	Cucurbita maxima (Rimu)	cmawgoB0263
Bhi08G000022	CmoCh13G003800	Cucurbita moschata (Rifu)	cmowgoB0254
Bhi08G000022	LsiUNG000570	Bottle gourd (USVL1VR-Ls)	lsiwgoB004
Bhi08G000022	Cp4.1LG00g01690	Cucurbita pepo (Zucchini)	cpewgoB0012
Bhi08G000022	CsGy1G014790	Cucumber (Gy14) v2	cgybwgoB046
Bhi08G000022	MELO3C025585.2	Melon (DHL92) v3.6.1	medwgoB176
Bhi08G000022	Carg26503	Silver-seed gourd	carwgoB1154
Bhi08G000022	CsaV3_1G015110	Cucumber (Chinese Long) v3	cucwgoB051
Bhi08G000022	Cla011060	Watermelon (97103) v1	wgowmB510
Bhi08G000022	Cla97C01G011330	Watermelon (97103) v2	wgowmbB451

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Bhi08G000022	Cucurbita maxima (Rimu)	cmawgoB0525
Bhi08G000022	Cucurbita moschata (Rifu)	cmowgoB0532
Bhi08G000022	Cucurbita pepo (Zucchini)	cpewgoB0050
Bhi08G000022	Melon (DHL92) v3.5.1	mewgoB426
Bhi08G000022	Silver-seed gourd	carwgoB0000