Carg26503 (gene) Silver-seed gourd | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Carg26503
Type	gene
Organism	Cucurbita argyrosperma (Silver-seed gourd)
Description	Nitrilase
Location	Cucurbita_argyrosperma_scaffold_255 : 51356 .. 56841 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CGAACTATCTGAAAACCACTGGACTCCATTGCAGCCATTCATTCCACTCTCATTCTCTGCTCCAATCTCAAGCTCAAGTTCAAGCTAAGAGGGGAAAAGGGCGATTTCGAATCATCCATCCACCATGGCGCTTGTGCCAACCACGGCCGCCACCGATACCCAACTGATAGCGGAGGTGGATATGGGCTCCGATGTATCTGTACCGACTGTTCGAGCCACCGTTGTTCAGGCCTCCACCATCTTCTACGACACTCCTGCCACTCTAGGTTTCCTTTCCTCACGTACACTTTAATTTTATCGTTGTGTAGTTCTCTTACTGTTGTTTCTTTGCTAACCATCTCTTGGCTTGGGATGGTCATTTCAGAGTTTGGAATGAGTTCCTGGTTGTTTTTTGGTGTGATGTTAGAAGCTTATCAGTTGTTTGAATGTTTTGTTTACTCTATGAAGTTTCTTCCCTTCTATCTGCAGATTTGCGAATAATGATTTAATTCATTTAAGCTATTATATCCTCGATTTTTTTTTTTCGTTTTTCATTGGTTTTCCTGTTTGCGGTCAATGTGAATGTAACTGTCCGTCCACAACTAAAGATATGGTACTCTTTGGGCTTTCTTTAAAACATGTTTGGTAGGGAGGTTTCCATACCCTTATAAAGAATCCTCCGAACGTGGGATCTCAACAGTCCACCCCTTCAGGGCTTAACGTCCTCGTTAGCACTCGTTCTTTTTTCCAATTGATGTAGGACGTTAGGCCCCGAAGTGGGTAGATTGTGAGATCCCAAATAGCATCAAAGCTGACGATGTGCCAGCAAGGAGGCTGATCTCTAAAGAGGGTGGACACGAGACGGTGTGCTAGCAAGGACCTTGGGACCTGAAGGGGGTGGATTGGAGGGTCCCACATCGATTGGAGAAGGGAACAAGTGGCAGCAAGGATGCTGGGCCCCGAAAGAAGATGGATTGTGAGATCCTACATTGGTTGGGAAGGAGAACGAAACATTATTTAGAACAATGTGGAAATCTCTCTCTAGCAAACGCGTTTTAAAAACCTTGAAGAGAAGTCCGAAAAGAAAAGTCCTAAGAAGGCAATATCTACTAGCAGTGAGCTTGGGCCGTTACCATAAATTTGTCAATGTCACCTAACATTGTACTCCTTATTGTTTTTTTCTCCTTAATTTCAGTGGATTGGAGAAATTATTTAATTCATAAATTCCAAAAGCGATTGATAGAGTTGTGGATCATCTCACGTAGATAGCAACTATTTTAGTAGATTGCACGCCATCTACTAGCCTATGACCATGTGGGTGTTGTTTAACTTGTAGTGAAAAACTAATTCTGGTTTACATGAACAGATAAGGCCGAGAGGTTGTTGGCTGAAGCTGCTGGATTTGGATCCCAGCTAGTTCTGTTTCCAGAAGCCTTTGTTGGTGGTTATCCCCGTGGATCAAATTTTGGTGTTTCCATTGGGAATAGAACTCCCAAGGGGAAAGAGGAATTCCGTAAATACCACGCAGCAGCGATCGATGTACCTGGTTAGTCTTTGTTTCCAATTCGACTTTGTTTAACTTAGTTGTGGTTGCATCTGTAAAGAGAACAATATTAGTGTCTTACCATGGATGATTTCAGGTTAAATGGACATTGTTAGGTGTAATAATAATACTCATTCTTGAAGGCCGACTTCGTTCCTCTACGAGTCTCTACCAAACTTTATTATATGGTCATATATTAATATCAAAGTCTTAAATCTTAGTATGCATCTTCTGTTCTTTATGAACTTTCAATCTTTAAGTAGATAATAGCTCATATTAGGACACTACAATAAGAACTTAGTGATGTCACTCTATTCCATCTGAAATACGAATCGGTTGTTCATGAAATGTGAAATGGGACTTTAGCCCTATTTTAATGGCTCCTGTAATTTACAATTGATGAATGAGAAACAATGCTTCAAAAATCTACAGGCATTTCCCGGGTCGAGTCTTTGCGAGTTCATCCCAAACAAAGCGAACCCGTTCTTTCTGGGTCTTGGTCAGGAAGATGTAGATAAAACGCCAATAATTTGATTCTTCTATGGCATAAGCTTTGAAGAAGAGAGTTATCAATCATCAGAAATTTCTCTTTTTTTCTCTCTTCCAAAATGAAACAAAATCCGTCATTAAATGACGAACCCCCTTATATAGATGATAGGACAAGGCTAAGGCAGTAATCTCGACGGAGATTAGAATGAACTTTGATGAATAAAAGACGAATCGGTAGAAATTCAAACCTTCCTACTCTTGCTTACACCTAAACATAATATTTTGAAGGTCTAAAAGCTATAGCCATTGTCTGTCTTAGGTGCTGCTTAGGTAGATGTAGTTCTAATTGAGTAAATGTTAATATGTTGATAACGATACATGCAGGTCCTGAAGTCGATCGATTGGCAGCAATGGCTGGAAAGTATAGGGTGCATTTGGTAATGGGGGTAATAGAGAGAGATGGCTACACACTGTACTGTACTGTTCTATTTTTTGATCCTCAAGGTCGTTACTTGGGTAAGCACAGGAAAGTGATGCCAACTGCATTGGAGCGGATAATCTGGGGATTTGGTGATGGATCAACAATTCCTGTTTTTGAGACTTCAATTGGAAAGATAGGTGCTGCCATTTGCTGGGAAAACAGAATGCCACTCCTAAGAACGGCTATGTATGCCAAAGGTTAGTTATTCTTGGCTTGAAATAATAAAATCCCTGACCTTCCCATTTTAAATCCAATTCCATTCATTTCGTTAGACTGTAAAGTGCTCATCAACTAAATTAGATGAAAATTTTCATTTAACAGAAGATTTTCCTATGGTTAAAGTTATACTGCTACCTTAACGATCAAAATTTTTACTTGAAAGTTATGTAACTTCCGTCCACACGTTCCTTTTCTTTTGCTCGACCTGTCGATCACTAGAAACTGACTTATATTTGTGGTCCTGTTTGTAGGCATTGAAATATATTGTGCACCTACTGCTGATTCTCGGGATACATGGCAAGCATCTATGACCCACATTGCTTTGGAGGGTGGTTGTTTTGTTTTGTCAGCCAACCAGTTCTGCCGAAGAAAAGACTACCCACCTCCACCAGATTATGTTTTTTCTGGCACAGAAGAGGAACTCAGCCCAGATTCTGTTGTTTGTGCTGGGGGTAGTGTCATCATATCGCCATCAGGTACAATCCTAGCTGGACCCAATTACGATGGAGAGGCACTTATTTCAGCAGATCTAGGTATGTCATCACGTCCTTCTACTTATAGTCATTAGAGCTATAGTTATTTACTACGTTTAGTGGTTTATATAGAAAAATTGTTTAATCTTTAGGAAACCTACTATTTGATTTAAGCCACTTTTTGGGGTTCTTTCTCCTAGATGATCTAATACTCCTGTCGATCGAGTAGTATGAAAACCATCATTCTAAGCTCTTATTCTATGCTTAGCTCTTTTTTAGTTACAATACGAGTGAATATGTAACAGTCCAAACCCACCGCTAGCAAATATTGTCCTCTTTGGCATTTTCCTTTTGTGTTTTCCCTCAAGGTTTTTAAACACGGCTGATAGGGAGAGATTGTTGGGAATAAACTTAAATGAAAAGAAAAAGGTAGTTTTTTAATTTCTTTATTTTTAGTAAGGCTTTTGAAATTTGAGTCGTAGGAGAAGGAAAAGGCTAACAATAATTTTAGGAGAGAGCAAGAGTTACACTGTTATTAAAGTACAGTGTAATAGAAAAGGGTTTTGAGAATTTCTTTGTCTCCTTGTCTTTTCTAGAAGTTGGTATTGGAGCGACTTTTAATTTCAACAGAGATTTCCACTCTTATAAATAATATTTTGTTCCTCTCTCCAATCAATGTGGGATCTCACAATTCACTCCCTTTTGGGGCCAGCGTCCTTGCTGGCCCCGTTCCCTTCTCCAATCGATGTGGGACCTTTGTTCCTCTTTCCAACCGACGTGGAATCTCACATAATAGCTTGTCTTCCCTGGTACAAATTGATTTTCCAACCAAGTGGGTAAGTTATGATGAACCAACCAACCATTTAAGATAGGGATGGCCTATATGTCTCTTGCTAACTAACAAGTTCCTTGGAGAATGGGGAGATAATCCGAGGTAGTTTTGTTGAACTATCCATGCCTTGGGCTGTGAAACTTTTCCATCTTTTGTGTGAGCAAAGGTTGAACTTGAGATTCCTAAGGTAAGCCTCTTAATTAGGTTGAAGGGTTTTCCTTCACTTACTAAGGTTGGGATTCCTAAGGTAAGTCCAGCTGATTTCATATGATTTTAGGATTAGCCAATAGTCCCACTATTTACCAAAGATTCTACAATGTGCTATTCTATAAAGGGGAGCTACCCATTTAAACAAGCAACTATTGAATGGATGATGAGAAGATTGAATTATGAAGAGCAAGAATGCTCTATATATATACAAGAGAATACAAAAAGATATTTAGAAAATCTACCTAAAAATCTGGAGAAAATCTGGTAACAAACCAGTAAACAAATCTAATCAAATCAAATCAAAAGTAAATCTAACTCTAACGGAGATTTAATTAGATACTAAATGACAGCAATCTAAACAAATACGTGATCTTTAACATGGAACAATATGATATATTATTGATCCATCCCAACTCTTAACAAGTTAAACTTTTTGATTTTTGATTGTTATGGAAAGTTTTTGCTATGAATTGCTTTTATATATAATTTGGATGCATAACTGAAACAGATCTTGGAGATATAGCACGGGCGAAATTTGATTTCGACGTGGTTGGTCATTATGCAAGGCCTGAAGTGCTTAGCTTGGTTGTGAGGGACCATCCTACAACCCCAGTTACTTTCACATCTGCAACTACAAAAACCGAAGATTCTTGTAAAAAATAGTAAGTTTATTGTCGAAGCTTCAATTTAGTTCCATTAATTGTTGTAATCTACATGTGTTGTCTATGTTTTACTGCTTCCTCATGCGTTCAGTGTTGTATTATATTCATCCTAATCATTCAGAACTGTGAATATTTCTGAATTTATATCAGCGGTGGATTTTAATATCATTTTTTCCTTCATTTCTAGCCTAGTTTGATTCATGATACTGTATTTAGGTGGATCAAGATATTGGTATATTGATGTTAGTGTCTAGAGAGATGAATAGAAACTTGGCGGAAGATTATTATGATGGGAAAGGGCAAAGATCATCTGAATCACCCAAACTAATATGGATTGGTCTAGTGGTCGAAAATGTTATTGCTCAGAGAAAACCTACCTAGAATTTAATATCCTACCGGTTACGTAGGCAACTAAATGTAGTAGGGATAGAGTTGTATTATGAGACTAGTTGAGATGCGTACAAGCTGAGTTGGACACTCGTAGATGTCTAAAGCAAAATAATAATATTGCCTTGATTTAGTTCAATCATTCTCAACTATGAAACCAAAACTGGTTCGACTCATAATCAAATTGATATGGGTTGGTCTCAAAATAATA

mRNA sequence

CGAACTATCTGAAAACCACTGGACTCCATTGCAGCCATTCATTCCACTCTCATTCTCTGCTCCAATCTCAAGCTCAAGTTCAAGCTAAGAGGGGAAAAGGGCGATTTCGAATCATCCATCCACCATGGCGCTTGTGCCAACCACGGCCGCCACCGATACCCAACTGATAGCGGAGGTGGATATGGGCTCCGATGTATCTGTACCGACTGTTCGAGCCACCGTTGTTCAGGCCTCCACCATCTTCTACGACACTCCTGCCACTCTAGATAAGGCCGAGAGGTTGTTGGCTGAAGCTGCTGGATTTGGATCCCAGCTAGTTCTGTTTCCAGAAGCCTTTGTTGGTGGTTATCCCCGTGGATCAAATTTTGGTGTTTCCATTGGGAATAGAACTCCCAAGGGGAAAGAGGAATTCCGTAAATACCACGCAGCAGCGATCGATGTACCTGGTCCTGAAGTCGATCGATTGGCAGCAATGGCTGGAAAGTATAGGGTGCATTTGGTAATGGGGGTAATAGAGAGAGATGGCTACACACTGTACTGTACTGTTCTATTTTTTGATCCTCAAGGTCGTTACTTGGGTAAGCACAGGAAAGTGATGCCAACTGCATTGGAGCGGATAATCTGGGGATTTGGTGATGGATCAACAATTCCTGTTTTTGAGACTTCAATTGGAAAGATAGGTGCTGCCATTTGCTGGGAAAACAGAATGCCACTCCTAAGAACGGCTATGTATGCCAAAGGCATTGAAATATATTGTGCACCTACTGCTGATTCTCGGGATACATGGCAAGCATCTATGACCCACATTGCTTTGGAGGGTGGTTGTTTTGTTTTGTCAGCCAACCAGTTCTGCCGAAGAAAAGACTACCCACCTCCACCAGATTATGTTTTTTCTGGCACAGAAGAGGAACTCAGCCCAGATTCTGTTGTTTGTGCTGGGGGTAGTGTCATCATATCGCCATCAGGTACAATCCTAGCTGGACCCAATTACGATGGAGAGGCACTTATTTCAGCAGATCTAGATCTTGGAGATATAGCACGGGCGAAATTTGATTTCGACGTGGTTGGTCATTATGCAAGGCCTGAAGTGCTTAGCTTGGTTGTGAGGGACCATCCTACAACCCCAGTTACTTTCACATCTGCAACTACAAAAACCGAAGATTCTTGTAAAAAATAGTAAGTTTATTGTCGAAGCTTCAATTTAGTTCCATTAATTGTTGTAATCTACATGTGTTGTCTATGTTTTACTGCTTCCTCATGCGTTCAGTGTTGTATTATATTCATCCTAATCATTCAGAACTGTGAATATTTCTGAATTTATATCAGCGGTGGATTTTAATATCATTTTTTCCTTCATTTCTAGCCTAGTTTGATTCATGATACTGTATTTAGGTGGATCAAGATATTGGTATATTGATGTTAGTGTCTAGAGAGATGAATAGAAACTTGGCGGAAGATTATTATGATGGGAAAGGGCAAAGATCATCTGAATCACCCAAACTAATATGGATTGGTCTAGTGGTCGAAAATGTTATTGCTCAGAGAAAACCTACCTAGAATTTAATATCCTACCGGTTACGTAGGCAACTAAATGTAGTAGGGATAGAGTTGTATTATGAGACTAGTTGAGATGCGTACAAGCTGAGTTGGACACTCGTAGATGTCTAAAGCAAAATAATAATATTGCCTTGATTTAGTTCAATCATTCTCAACTATGAAACCAAAACTGGTTCGACTCATAATCAAATTGATATGGGTTGGTCTCAAAATAATA

Coding sequence (CDS)

ATGGCGCTTGTGCCAACCACGGCCGCCACCGATACCCAACTGATAGCGGAGGTGGATATGGGCTCCGATGTATCTGTACCGACTGTTCGAGCCACCGTTGTTCAGGCCTCCACCATCTTCTACGACACTCCTGCCACTCTAGATAAGGCCGAGAGGTTGTTGGCTGAAGCTGCTGGATTTGGATCCCAGCTAGTTCTGTTTCCAGAAGCCTTTGTTGGTGGTTATCCCCGTGGATCAAATTTTGGTGTTTCCATTGGGAATAGAACTCCCAAGGGGAAAGAGGAATTCCGTAAATACCACGCAGCAGCGATCGATGTACCTGGTCCTGAAGTCGATCGATTGGCAGCAATGGCTGGAAAGTATAGGGTGCATTTGGTAATGGGGGTAATAGAGAGAGATGGCTACACACTGTACTGTACTGTTCTATTTTTTGATCCTCAAGGTCGTTACTTGGGTAAGCACAGGAAAGTGATGCCAACTGCATTGGAGCGGATAATCTGGGGATTTGGTGATGGATCAACAATTCCTGTTTTTGAGACTTCAATTGGAAAGATAGGTGCTGCCATTTGCTGGGAAAACAGAATGCCACTCCTAAGAACGGCTATGTATGCCAAAGGCATTGAAATATATTGTGCACCTACTGCTGATTCTCGGGATACATGGCAAGCATCTATGACCCACATTGCTTTGGAGGGTGGTTGTTTTGTTTTGTCAGCCAACCAGTTCTGCCGAAGAAAAGACTACCCACCTCCACCAGATTATGTTTTTTCTGGCACAGAAGAGGAACTCAGCCCAGATTCTGTTGTTTGTGCTGGGGGTAGTGTCATCATATCGCCATCAGGTACAATCCTAGCTGGACCCAATTACGATGGAGAGGCACTTATTTCAGCAGATCTAGATCTTGGAGATATAGCACGGGCGAAATTTGATTTCGACGTGGTTGGTCATTATGCAAGGCCTGAAGTGCTTAGCTTGGTTGTGAGGGACCATCCTACAACCCCAGTTACTTTCACATCTGCAACTACAAAAACCGAAGATTCTTGTAAAAAATAG

Protein sequence

MALVPTTAATDTQLIAEVDMGSDVSVPTVRATVVQASTIFYDTPATLDKAERLLAEAAGFGSQLVLFPEAFVGGYPRGSNFGVSIGNRTPKGKEEFRKYHAAAIDVPGPEVDRLAAMAGKYRVHLVMGVIERDGYTLYCTVLFFDPQGRYLGKHRKVMPTALERIIWGFGDGSTIPVFETSIGKIGAAICWENRMPLLRTAMYAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSANQFCRRKDYPPPPDYVFSGTEEELSPDSVVCAGGSVIISPSGTILAGPNYDGEALISADLDLGDIARAKFDFDVVGHYARPEVLSLVVRDHPTTPVTFTSATTKTEDSCKK

Homology

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BLAST of Carg26503 vs. NCBI nr
Match: XP_023520715.1 (bifunctional nitrilase/nitrile hydratase NIT4A [Cucurbita pepo subsp. pepo])

HSP 1 Score: 708.4 bits (1827), Expect = 1.2e-200
Identity = 348/350 (99.43%), Postives = 350/350 (100.00%), Query Frame = 0


Query: 1   MALVPTTAATDTQLIAEVDMGSDVSVPTVRATVVQASTIFYDTPATLDKAERLLAEAAGF 60
           MALVPTTAATDTQLIAEVDMGSDVSVPTVRATVVQASTIFYDTPATLDKAERLLAEAAGF
Sbjct: 1   MALVPTTAATDTQLIAEVDMGSDVSVPTVRATVVQASTIFYDTPATLDKAERLLAEAAGF 60

Query: 61  GSQLVLFPEAFVGGYPRGSNFGVSIGNRTPKGKEEFRKYHAAAIDVPGPEVDRLAAMAGK 120
           GSQLV+FPEAFVGGYPRGSNFGVSIGNRTPKGKEEFRKYHAAAIDVPGPEVDRLAAMAGK
Sbjct: 61  GSQLVVFPEAFVGGYPRGSNFGVSIGNRTPKGKEEFRKYHAAAIDVPGPEVDRLAAMAGK 120

Query: 121 YRVHLVMGVIERDGYTLYCTVLFFDPQGRYLGKHRKVMPTALERIIWGFGDGSTIPVFET 180
           YRVHLVMGVIERDGYTLYCTVLFFDPQGRYLGKHRKVMPTALERIIWGFGDGSTIPVFET
Sbjct: 121 YRVHLVMGVIERDGYTLYCTVLFFDPQGRYLGKHRKVMPTALERIIWGFGDGSTIPVFET 180

Query: 181 SIGKIGAAICWENRMPLLRTAMYAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSAN 240
           SIGKIGAAICWENRMPLLRTAMYAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSAN
Sbjct: 181 SIGKIGAAICWENRMPLLRTAMYAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSAN 240

Query: 241 QFCRRKDYPPPPDYVFSGTEEELSPDSVVCAGGSVIISPSGTILAGPNYDGEALISADLD 300
           QFCRRKDYPPPPDYVFSGTEEELSPDSVVCAGGSVIISPSGTILAGPNYDGEALISADLD
Sbjct: 241 QFCRRKDYPPPPDYVFSGTEEELSPDSVVCAGGSVIISPSGTILAGPNYDGEALISADLD 300

Query: 301 LGDIARAKFDFDVVGHYARPEVLSLVVRDHPTTPVTFTSATTKTEDSCKK 351
           LGDIARAKFDFDVVGHYARPEVLSLVVRDHPTTPVTFTSA+TKTEDSCKK
Sbjct: 301 LGDIARAKFDFDVVGHYARPEVLSLVVRDHPTTPVTFTSASTKTEDSCKK 350

[Close]

BLAST of Carg26503 vs. NCBI nr
Match: XP_022927562.1 (bifunctional nitrilase/nitrile hydratase NIT4A [Cucurbita moschata])

HSP 1 Score: 704.1 bits (1816), Expect = 2.3e-199
Identity = 346/349 (99.14%), Postives = 347/349 (99.43%), Query Frame = 0


Query: 1   MALVPTTAATDTQLIAEVDMGSDVSVPTVRATVVQASTIFYDTPATLDKAERLLAEAAGF 60
           MALVPTTAA DTQLIAEVDMGSDVSVPTVRATVVQASTIFYDTPATLDKAERLLAEAAGF
Sbjct: 1   MALVPTTAAADTQLIAEVDMGSDVSVPTVRATVVQASTIFYDTPATLDKAERLLAEAAGF 60

Query: 61  GSQLVLFPEAFVGGYPRGSNFGVSIGNRTPKGKEEFRKYHAAAIDVPGPEVDRLAAMAGK 120
           GSQLV+FPEAFVGGYPRGSNFGVSIGNRTPKGKEEFRKYHAAAIDVPGPEVDRLAAMAGK
Sbjct: 61  GSQLVVFPEAFVGGYPRGSNFGVSIGNRTPKGKEEFRKYHAAAIDVPGPEVDRLAAMAGK 120

Query: 121 YRVHLVMGVIERDGYTLYCTVLFFDPQGRYLGKHRKVMPTALERIIWGFGDGSTIPVFET 180
           YRVHLVMGVIERDGYTLYCTVLFFDPQGRYLGKHRKVMPTALERIIWGFGDGSTIPVFET
Sbjct: 121 YRVHLVMGVIERDGYTLYCTVLFFDPQGRYLGKHRKVMPTALERIIWGFGDGSTIPVFET 180

Query: 181 SIGKIGAAICWENRMPLLRTAMYAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSAN 240
           SIGKIGAAICWENRMPLLRTAMYAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSAN
Sbjct: 181 SIGKIGAAICWENRMPLLRTAMYAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSAN 240

Query: 241 QFCRRKDYPPPPDYVFSGTEEELSPDSVVCAGGSVIISPSGTILAGPNYDGEALISADLD 300
           QFCRRKDYPPPPDYVFSGTEEELSPDSVVCAGGSVIISPSGTILAGPNYDGEALISADLD
Sbjct: 241 QFCRRKDYPPPPDYVFSGTEEELSPDSVVCAGGSVIISPSGTILAGPNYDGEALISADLD 300

Query: 301 LGDIARAKFDFDVVGHYARPEVLSLVVRDHPTTPVTFTSATTKTEDSCK 350
           LGDIARAKFDFDVVGHYARPEVLSLVVRDHPTTPVTFTSAT KTEDSCK
Sbjct: 301 LGDIARAKFDFDVVGHYARPEVLSLVVRDHPTTPVTFTSATAKTEDSCK 349

[Close]

BLAST of Carg26503 vs. NCBI nr
Match: XP_022973146.1 (bifunctional nitrilase/nitrile hydratase NIT4A [Cucurbita maxima])

HSP 1 Score: 702.2 bits (1811), Expect = 8.8e-199
Identity = 345/350 (98.57%), Postives = 347/350 (99.14%), Query Frame = 0


Query: 1   MALVPTTAATDTQLIAEVDMGSDVSVPTVRATVVQASTIFYDTPATLDKAERLLAEAAGF 60
           MALVPTTAA DTQLIAEVDMGSDVSVPTVRATVVQASTIFYDTPATLDKAERLLAEAAGF
Sbjct: 1   MALVPTTAANDTQLIAEVDMGSDVSVPTVRATVVQASTIFYDTPATLDKAERLLAEAAGF 60

Query: 61  GSQLVLFPEAFVGGYPRGSNFGVSIGNRTPKGKEEFRKYHAAAIDVPGPEVDRLAAMAGK 120
           GSQLV+FPEAFVGGYPRGSNFGVSIGNRTPKGKEEFRKYHAAAIDVPGPEVDRLAAMAGK
Sbjct: 61  GSQLVVFPEAFVGGYPRGSNFGVSIGNRTPKGKEEFRKYHAAAIDVPGPEVDRLAAMAGK 120

Query: 121 YRVHLVMGVIERDGYTLYCTVLFFDPQGRYLGKHRKVMPTALERIIWGFGDGSTIPVFET 180
           YRVHLVMGVIERDGYTLYCTVLFFDPQGRYLGKHRKVMPTALERIIWGFGDGSTIPVFET
Sbjct: 121 YRVHLVMGVIERDGYTLYCTVLFFDPQGRYLGKHRKVMPTALERIIWGFGDGSTIPVFET 180

Query: 181 SIGKIGAAICWENRMPLLRTAMYAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSAN 240
           SIGKIGAAICWENRMPLLRTAMYAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSAN
Sbjct: 181 SIGKIGAAICWENRMPLLRTAMYAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSAN 240

Query: 241 QFCRRKDYPPPPDYVFSGTEEELSPDSVVCAGGSVIISPSGTILAGPNYDGEALISADLD 300
           QFCRRKDYPPPPDYVFSGTEEELSPDSVVCAGGSVIISPSGTILAGPNYDGEALISADLD
Sbjct: 241 QFCRRKDYPPPPDYVFSGTEEELSPDSVVCAGGSVIISPSGTILAGPNYDGEALISADLD 300

Query: 301 LGDIARAKFDFDVVGHYARPEVLSLVVRDHPTTPVTFTSATTKTEDSCKK 351
           LGDIARAKFDFDVVGHYARPEVLSLVVRDHPTTPVTFTSA+ KTEDSC K
Sbjct: 301 LGDIARAKFDFDVVGHYARPEVLSLVVRDHPTTPVTFTSASKKTEDSCNK 350

[Close]

BLAST of Carg26503 vs. NCBI nr
Match: XP_004139500.1 (PREDICTED: bifunctional nitrilase/nitrile hydratase NIT4A [Cucumis sativus] >KGN64959.1 hypothetical protein Csa_1G168360 [Cucumis sativus])

HSP 1 Score: 686.8 bits (1771), Expect = 3.8e-194
Identity = 336/350 (96.00%), Postives = 343/350 (98.00%), Query Frame = 0


Query: 1   MALVPTTAATDTQLIAEVDMGSDVSVPTVRATVVQASTIFYDTPATLDKAERLLAEAAGF 60
           MALVPTTA TD+QLIAEVDMGSD SV  VRATVVQASTIFYDTPATLDKAERLLAEAAGF
Sbjct: 1   MALVPTTAPTDSQLIAEVDMGSDSSVMNVRATVVQASTIFYDTPATLDKAERLLAEAAGF 60

Query: 61  GSQLVLFPEAFVGGYPRGSNFGVSIGNRTPKGKEEFRKYHAAAIDVPGPEVDRLAAMAGK 120
           GSQLV+FPEAFVGGYPRGSNFGVSIGNRTPKGKEEFRKYHA+AIDVPGPEVDRLAAMAGK
Sbjct: 61  GSQLVVFPEAFVGGYPRGSNFGVSIGNRTPKGKEEFRKYHASAIDVPGPEVDRLAAMAGK 120

Query: 121 YRVHLVMGVIERDGYTLYCTVLFFDPQGRYLGKHRKVMPTALERIIWGFGDGSTIPVFET 180
           YRVHLVMGVIERDGYTLYCTVLFFDPQGRYLGKHRKVMPTALERIIWGFGDGSTIPVFET
Sbjct: 121 YRVHLVMGVIERDGYTLYCTVLFFDPQGRYLGKHRKVMPTALERIIWGFGDGSTIPVFET 180

Query: 181 SIGKIGAAICWENRMPLLRTAMYAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSAN 240
           SIGKIGAAICWENRMPLLRTAMYAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSAN
Sbjct: 181 SIGKIGAAICWENRMPLLRTAMYAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSAN 240

Query: 241 QFCRRKDYPPPPDYVFSGTEEELSPDSVVCAGGSVIISPSGTILAGPNYDGEALISADLD 300
           QFCRRKDYPPPP+YVFSGTEEEL+PDSVVCAGGS IISPSGTILAGPNYDGEALISADLD
Sbjct: 241 QFCRRKDYPPPPEYVFSGTEEELTPDSVVCAGGSAIISPSGTILAGPNYDGEALISADLD 300

Query: 301 LGDIARAKFDFDVVGHYARPEVLSLVVRDHPTTPVTFTSATTKTEDSCKK 351
           LG+IARAKFDFDVVGHYARPEVLSLVVRDHPTTPVTFTS +TK EDSCKK
Sbjct: 301 LGEIARAKFDFDVVGHYARPEVLSLVVRDHPTTPVTFTSTSTKVEDSCKK 350

[Close]

BLAST of Carg26503 vs. NCBI nr
Match: XP_008463648.1 (PREDICTED: bifunctional nitrilase/nitrile hydratase NIT4A [Cucumis melo])

HSP 1 Score: 683.7 bits (1763), Expect = 3.3e-193
Identity = 335/350 (95.71%), Postives = 341/350 (97.43%), Query Frame = 0


Query: 1   MALVPTTAATDTQLIAEVDMGSDVSVPTVRATVVQASTIFYDTPATLDKAERLLAEAAGF 60
           MALVPTTA TD QLIAEVDMG+D SV TVRATVVQASTIFYDTPATLDKAERLLAEAAGF
Sbjct: 1   MALVPTTAPTDPQLIAEVDMGTDSSVMTVRATVVQASTIFYDTPATLDKAERLLAEAAGF 60

Query: 61  GSQLVLFPEAFVGGYPRGSNFGVSIGNRTPKGKEEFRKYHAAAIDVPGPEVDRLAAMAGK 120
           GSQLV+FPEAFVGGYPRGSNFGVSIGNRTPKGKEEFRKYHAAAIDVPGPEVDRLAAMAGK
Sbjct: 61  GSQLVVFPEAFVGGYPRGSNFGVSIGNRTPKGKEEFRKYHAAAIDVPGPEVDRLAAMAGK 120

Query: 121 YRVHLVMGVIERDGYTLYCTVLFFDPQGRYLGKHRKVMPTALERIIWGFGDGSTIPVFET 180
           YRVHLVMGVIERDGYTLYCTVLFFDPQGRYLGKHRKVMPTALERIIWGFGDGSTIPVFET
Sbjct: 121 YRVHLVMGVIERDGYTLYCTVLFFDPQGRYLGKHRKVMPTALERIIWGFGDGSTIPVFET 180

Query: 181 SIGKIGAAICWENRMPLLRTAMYAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSAN 240
           SIGKIGAAICWENRMPLLRTAMYAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSAN
Sbjct: 181 SIGKIGAAICWENRMPLLRTAMYAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSAN 240

Query: 241 QFCRRKDYPPPPDYVFSGTEEELSPDSVVCAGGSVIISPSGTILAGPNYDGEALISADLD 300
           QFCRRKDYPPPP+YVFSGTEEEL+PDSVVCAGGS IISPSGTILAGPNYDGEALISADLD
Sbjct: 241 QFCRRKDYPPPPEYVFSGTEEELTPDSVVCAGGSAIISPSGTILAGPNYDGEALISADLD 300

Query: 301 LGDIARAKFDFDVVGHYARPEVLSLVVRDHPTTPVTFTSATTKTEDSCKK 351
           LG+IARAKFDFDVVGHYARPEVLSLVVRDHP  PVTFTS +TK EDSCKK
Sbjct: 301 LGEIARAKFDFDVVGHYARPEVLSLVVRDHPAIPVTFTSTSTKAEDSCKK 350

[Close]

BLAST of Carg26503 vs. TAIR10
Match: AT5G22300.1 (nitrilase 4)

HSP 1 Score: 548.9 bits (1413), Expect = 2.3e-156
Identity = 266/344 (77.33%), Postives = 302/344 (87.79%), Query Frame = 0


Query: 7   TAATDT---QLIAEVDMGSDVSVPTVRATVVQASTIFYDTPATLDKAERLLAEAAGFGSQ 66
           TAA  T   Q+  E+DM +  S   VRATVVQAST+FYDTPATLDKAERLL+EAA  GSQ
Sbjct: 11  TAAPQTNGHQIFPEIDMSAGDSSSIVRATVVQASTVFYDTPATLDKAERLLSEAAENGSQ 70

Query: 67  LVLFPEAFVGGYPRGSNFGVSIGNRTPKGKEEFRKYHAAAIDVPGPEVDRLAAMAGKYRV 126
           LV+FPEAF+GGYPRGS F ++IG+RT KG+++FRKYHA+AIDVPGPEV+RLA MA KY+V
Sbjct: 71  LVVFPEAFIGGYPRGSTFELAIGSRTAKGRDDFRKYHASAIDVPGPEVERLALMAKKYKV 130

Query: 127 HLVMGVIERDGYTLYCTVLFFDPQGRYLGKHRKVMPTALERIIWGFGDGSTIPVFETSIG 186
           +LVMGVIER+GYTLYCTVLFFD QG +LGKHRK+MPTALER IWGFGDGSTIPVF+T IG
Sbjct: 131 YLVMGVIEREGYTLYCTVLFFDSQGLFLGKHRKLMPTALERCIWGFGDGSTIPVFDTPIG 190

Query: 187 KIGAAICWENRMPLLRTAMYAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSANQFC 246
           KIGAAICWENRMP LRTAMYAKGIEIYCAPTADSR+TW ASMTHIALEGGCFVLSANQFC
Sbjct: 191 KIGAAICWENRMPSLRTAMYAKGIEIYCAPTADSRETWLASMTHIALEGGCFVLSANQFC 250

Query: 247 RRKDYPPPPDYVFSGTEEELSPDSVVCAGGSVIISPSGTILAGPNYDGEALISADLDLGD 306
           RRKDYP PP+Y+FSG+EE L+PDSVVCAGGS IISP G +LAGPNY GEALI+ADLDLGD
Sbjct: 251 RRKDYPSPPEYMFSGSEESLTPDSVVCAGGSSIISPLGIVLAGPNYRGEALITADLDLGD 310

Query: 307 IARAKFDFDVVGHYARPEVLSLVVRDHPTTPVTFTSATTKTEDS 348
           IARAKFDFDVVGHY+RPEV SL +R+HP   V+F ++    ++S
Sbjct: 311 IARAKFDFDVVGHYSRPEVFSLNIREHPRKAVSFKTSKVMEDES 354

[Close]

BLAST of Carg26503 vs. TAIR10
Match: AT3G44300.1 (nitrilase 2)

HSP 1 Score: 471.1 bits (1211), Expect = 6.0e-133
Identity = 217/322 (67.39%), Postives = 262/322 (81.37%), Query Frame = 0


Query: 29  VRATVVQASTIFYDTPATLDKAERLLAEAAGFGSQLVLFPEAFVGGYPRGSNFGVSIGNR 88
           VRAT+VQAST++ DTPATL+KA + + EAA  GS+LV+FPEAF+GGYPRG  FG+ +G  
Sbjct: 18  VRATIVQASTVYNDTPATLEKANKFIVEAASKGSELVVFPEAFIGGYPRGFRFGLGVGVH 77

Query: 89  TPKGKEEFRKYHAAAIDVPGPEVDRLAAMAGKYRVHLVMGVIERDGYTLYCTVLFFDPQG 148
             +G++EFRKYHA+AI VPGPEV++LA +AGK  V+LVMG IE+DGYTLYCT LFF PQG
Sbjct: 78  NEEGRDEFRKYHASAIKVPGPEVEKLAELAGKNNVYLVMGAIEKDGYTLYCTALFFSPQG 137

Query: 149 RYLGKHRKVMPTALERIIWGFGDGSTIPVFETSIGKIGAAICWENRMPLLRTAMYAKGIE 208
           ++LGKHRK+MPT+LER IWG GDGSTIPV++T IGK+GAAICWENRMPL RTA+YAKGIE
Sbjct: 138 QFLGKHRKLMPTSLERCIWGQGDGSTIPVYDTPIGKLGAAICWENRMPLYRTALYAKGIE 197

Query: 209 IYCAPTADSRDTWQASMTHIALEGGCFVLSANQFCRRKDYPPPPDYVFSGTEEELSPDSV 268
           +YCAPTAD    WQ+SM HIA+EGGCFVLSA QFC RKD+P  PDY+F+   ++  PDS+
Sbjct: 198 LYCAPTADGSKEWQSSMLHIAIEGGCFVLSACQFCLRKDFPDHPDYLFTDWYDDKEPDSI 257

Query: 269 VCAGGSVIISPSGTILAGPNYDGEALISADLDLGDIARAKFDFDVVGHYARPEVLSLVVR 328
           V  GGSVIISP G +LAGPN++ E LI+ADLDLGD+ARAK  FD VGHY+RP+VL L V 
Sbjct: 258 VSQGGSVIISPLGQVLAGPNFESEGLITADLDLGDVARAKLYFDSVGHYSRPDVLHLTVN 317

Query: 329 DHPTTPVTFTSATTKTEDSCKK 351
           +HP  PVTF S   K ED   K
Sbjct: 318 EHPKKPVTFISKVEKAEDDSNK 339

[Close]

BLAST of Carg26503 vs. TAIR10
Match: AT3G44320.1 (nitrilase 3)

HSP 1 Score: 470.7 bits (1210), Expect = 7.9e-133
Identity = 217/323 (67.18%), Postives = 262/323 (81.11%), Query Frame = 0


Query: 28  TVRATVVQASTIFYDTPATLDKAERLLAEAAGFGSQLVLFPEAFVGGYPRGSNFGVSIGN 87
           TVR T+VQ+ST++ DTPATLDKAE+ + EAA  G++LVLFPEAF+GGYPRG  FG+++G 
Sbjct: 24  TVRVTIVQSSTVYNDTPATLDKAEKFIVEAASKGAKLVLFPEAFIGGYPRGFRFGLAVGV 83

Query: 88  RTPKGKEEFRKYHAAAIDVPGPEVDRLAAMAGKYRVHLVMGVIERDGYTLYCTVLFFDPQ 147
              +G++EFR YHA+AI VPGPEV+RLA +AGK  VHLVMG IE+DGYTLYCT LFF PQ
Sbjct: 84  HNEEGRDEFRNYHASAIKVPGPEVERLAELAGKNNVHLVMGAIEKDGYTLYCTALFFSPQ 143

Query: 148 GRYLGKHRKVMPTALERIIWGFGDGSTIPVFETSIGKIGAAICWENRMPLLRTAMYAKGI 207
           G++LGKHRKVMPT+LER IWG GDGSTIPV++T IGKIGAAICWENRMPL RTA+YAKGI
Sbjct: 144 GQFLGKHRKVMPTSLERCIWGQGDGSTIPVYDTPIGKIGAAICWENRMPLYRTALYAKGI 203

Query: 208 EIYCAPTADSRDTWQASMTHIALEGGCFVLSANQFCRRKDYPPPPDYVFSGTEEELSPDS 267
           EIYCAPTAD    WQASM HIA+EGGCFVLSA+QFC+R+++P  PDY+F+   +    D 
Sbjct: 204 EIYCAPTADYSLEWQASMIHIAVEGGCFVLSAHQFCKRREFPEHPDYLFNDIVDTKEHDP 263

Query: 268 VVCAGGSVIISPSGTILAGPNYDGEALISADLDLGDIARAKFDFDVVGHYARPEVLSLVV 327
            V  GGSVIISP G +LAGPNY+ E L++ADLDLGDIARAK  FDVVGHY++P++ +L V
Sbjct: 264 TVSGGGSVIISPLGKVLAGPNYESEGLVTADLDLGDIARAKLYFDVVGHYSKPDIFNLTV 323

Query: 328 RDHPTTPVTFTSATTKTEDSCKK 351
            +HP  PVTF +   K ED   K
Sbjct: 324 NEHPKKPVTFMTKVEKAEDESNK 346

[Close]

BLAST of Carg26503 vs. TAIR10
Match: AT3G44310.1 (nitrilase 1)

HSP 1 Score: 457.6 bits (1176), Expect = 6.9e-129
Identity = 211/323 (65.33%), Postives = 260/323 (80.50%), Query Frame = 0


Query: 28  TVRATVVQASTIFYDTPATLDKAERLLAEAAGFGSQLVLFPEAFVGGYPRGSNFGVSIGN 87
           TVR T+VQ+ST++ DTPAT+DKAE+ + EAA  G++LVLFPE F+GGYPRG  FG+++G 
Sbjct: 24  TVRVTIVQSSTVYNDTPATIDKAEKYIVEAASKGAELVLFPEGFIGGYPRGFRFGLAVGV 83

Query: 88  RTPKGKEEFRKYHAAAIDVPGPEVDRLAAMAGKYRVHLVMGVIERDGYTLYCTVLFFDPQ 147
              +G++EFRKYHA+AI VPGPEV RLA +A K  V+LVMG IE++GYTLYCTVLFF PQ
Sbjct: 84  HNEEGRDEFRKYHASAIHVPGPEVARLADVARKNHVYLVMGAIEKEGYTLYCTVLFFSPQ 143

Query: 148 GRYLGKHRKVMPTALERIIWGFGDGSTIPVFETSIGKIGAAICWENRMPLLRTAMYAKGI 207
           G++LGKHRK+MPT+LER IWG GDGSTIPV++T IGK+GAAICWENRMPL RTA+YAKGI
Sbjct: 144 GQFLGKHRKLMPTSLERCIWGQGDGSTIPVYDTPIGKLGAAICWENRMPLYRTALYAKGI 203

Query: 208 EIYCAPTADSRDTWQASMTHIALEGGCFVLSANQFCRRKDYPPPPDYVFSGTEEELSPDS 267
           E+YCAPTAD    WQ+SM HIA+EGGCFVLSA QFC+RK +P  PDY+F+   ++   DS
Sbjct: 204 ELYCAPTADGSKEWQSSMLHIAIEGGCFVLSACQFCQRKHFPDHPDYLFTDWYDDKEHDS 263

Query: 268 VVCAGGSVIISPSGTILAGPNYDGEALISADLDLGDIARAKFDFDVVGHYARPEVLSLVV 327
           +V  GGSVIISP G +LAGPN++ E L++AD+DLGDIARAK  FD VGHY+RP+VL L V
Sbjct: 264 IVSQGGSVIISPLGQVLAGPNFESEGLVTADIDLGDIARAKLYFDSVGHYSRPDVLHLTV 323

Query: 328 RDHPTTPVTFTSATTKTEDSCKK 351
            +HP   VTF +   K ED   K
Sbjct: 324 NEHPRKSVTFVTKVEKAEDDSNK 346

[Close]

BLAST of Carg26503 vs. TAIR10
Match: AT2G27450.2 (nitrilase-like protein 1)

HSP 1 Score: 78.6 bits (192), Expect = 8.7e-15
Identity = 75/289 (25.95%), Postives = 118/289 (40.83%), Query Frame = 0


Query: 53  LLAEAAGFGSQLVLFPEAFVGGYPRGSNFGVSIGNRTPKGKEEFRKYHAAAIDVPGPEVD 112
           L+ EA   G+ ++L  E F G Y                 +E+F  +  A      P + 
Sbjct: 60  LVREAHAKGANIILIQELFEGYY------------FCQAQREDF--FKRAKPYKNHPTIA 119

Query: 113 RLAAMAGKYRVHLVMGVIERDGYTLYCTVLFFDPQGRYLGKHRKVM----PTALERIIWG 172
           R+  +A +  V + +   E      Y ++   D  G  LG +RK      P   E+  + 
Sbjct: 120 RMQKLAKELGVVIPVSFFEEANTAHYNSIAIIDADGTDLGIYRKSHIPDGPGYQEKFYFN 179

Query: 173 FGDGSTIPVFETSIGKIGAAICWENRMPLLRTAMYAKGIEIYCAPTA----------DSR 232
            GD +   VF+T   KIG AICW+   P    AM  +G EI   PTA          DSR
Sbjct: 180 PGD-TGFKVFQTKFAKIGVAICWDQWFPEAARAMVLQGAEILFYPTAIGSEPQDQGLDSR 239

Query: 233 DTWQASMTHIALEGGCFVLSANQFCRRKDYPPPPDYVFSGTEEELSPDSVVCAGGSVIIS 292
           D W+  M   A      ++++N+  +               E E  P  +   G S I  
Sbjct: 240 DHWRRVMQGHAGANVVPLVASNRIGKEI------------IETEHGPSQITFYGTSFIAG 299

Query: 293 PSGTILAGPNYDGEALISADLDLGDIARAKFDFDVVGHYARPEVLSLVV 328
           P+G I+A  +   EA++ A  DL D+ ++K     V    RP++  +++
Sbjct: 300 PTGEIVAEADDKSEAVLVAQFDL-DMIKSKRQSWGVFRDRRPDLYKVLL 320

[Close]

BLAST of Carg26503 vs. Swiss-Prot
Match: sp|Q5QGZ8|NRL4A_LUPAN (Bifunctional nitrilase/nitrile hydratase NIT4A OS=Lupinus angustifolius OX=3871 GN=NIT4A PE=1 SV=1)

HSP 1 Score: 624.4 bits (1609), Expect = 7.7e-178
Identity = 299/349 (85.67%), Postives = 325/349 (93.12%), Query Frame = 0


Query: 1   MALVPTTAATDTQLIAEVDMGSDVSVPTVRATVVQASTIFYDTPATLDKAERLLAEAAGF 60
           MALV T    D  L AEV+M SD + PTVRATVVQASTIFYDTPATLDKAERLLAEAA +
Sbjct: 1   MALVTTPTVNDGPLFAEVNMSSDFNAPTVRATVVQASTIFYDTPATLDKAERLLAEAASY 60

Query: 61  GSQLVLFPEAFVGGYPRGSNFGVSIGNRTPKGKEEFRKYHAAAIDVPGPEVDRLAAMAGK 120
           G+Q+V+FPEAF+GGYPRGSNFGVSIGNRT KGKE+FRKYH+AAIDVPGPEVDRLAA+AGK
Sbjct: 61  GAQIVVFPEAFIGGYPRGSNFGVSIGNRTAKGKEDFRKYHSAAIDVPGPEVDRLAALAGK 120

Query: 121 YRVHLVMGVIERDGYTLYCTVLFFDPQGRYLGKHRKVMPTALERIIWGFGDGSTIPVFET 180
           Y+V+LVMGVIERDGYTLYCTVLFF  QGRYLGKHRK+MPTALERIIWGFGDGSTIPVFET
Sbjct: 121 YKVYLVMGVIERDGYTLYCTVLFFGAQGRYLGKHRKLMPTALERIIWGFGDGSTIPVFET 180

Query: 181 SIGKIGAAICWENRMPLLRTAMYAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSAN 240
            IGKIGAAICWEN+MPLLRTAMYAKG+EIYCAPTADSR+ WQASMTHIALEGGCFVLSAN
Sbjct: 181 PIGKIGAAICWENKMPLLRTAMYAKGVEIYCAPTADSREVWQASMTHIALEGGCFVLSAN 240

Query: 241 QFCRRKDYPPPPDYVFSGTEEELSPDSVVCAGGSVIISPSGTILAGPNYDGEALISADLD 300
           QFCRR+DYPPPP+YVF GTEE L+PDSVVCAGGSVIISPSG +LAGP+Y+GEALISADLD
Sbjct: 241 QFCRRRDYPPPPEYVFEGTEENLTPDSVVCAGGSVIISPSGAVLAGPSYEGEALISADLD 300

Query: 301 LGDIARAKFDFDVVGHYARPEVLSLVVRDHPTTPVTFTSATTKTEDSCK 350
           LG+IARAKFDFDVVGHY+RPEVLSLVV+DHPT PVTFTSA+TK ED  K
Sbjct: 301 LGEIARAKFDFDVVGHYSRPEVLSLVVKDHPTNPVTFTSASTKIEDKTK 349

[Close]

BLAST of Carg26503 vs. Swiss-Prot
Match: sp|Q42965|NRL4A_TOBAC (Bifunctional nitrilase/nitrile hydratase NIT4A OS=Nicotiana tabacum OX=4097 GN=NIT4A PE=2 SV=1)

HSP 1 Score: 620.9 bits (1600), Expect = 8.5e-177
Identity = 299/349 (85.67%), Postives = 324/349 (92.84%), Query Frame = 0


Query: 1   MALVPTTAATDTQLIAEVDMGSDVSVPTVRATVVQASTIFYDTPATLDKAERLLAEAAGF 60
           MALVPT A  +  L AEVDMG + S PTVRATVVQASTIFYDTPATL KAERLLAEAA +
Sbjct: 1   MALVPTPAVNEGPLFAEVDMGDNSSTPTVRATVVQASTIFYDTPATLVKAERLLAEAASY 60

Query: 61  GSQLVLFPEAFVGGYPRGSNFGVSIGNRTPKGKEEFRKYHAAAIDVPGPEVDRLAAMAGK 120
           G+QLV+FPEAF+GGYPRGS FGVSIGNRT KGKEEFRKYHA+AIDVPGPEVDRLAAMAGK
Sbjct: 61  GAQLVVFPEAFIGGYPRGSTFGVSIGNRTAKGKEEFRKYHASAIDVPGPEVDRLAAMAGK 120

Query: 121 YRVHLVMGVIERDGYTLYCTVLFFDPQGRYLGKHRKVMPTALERIIWGFGDGSTIPVFET 180
           Y+V+LVMGVIERDGYTLYCTVLFFD QG +LGKHRK+MPTALERIIWGFGDGSTIPV++T
Sbjct: 121 YKVYLVMGVIERDGYTLYCTVLFFDSQGHFLGKHRKIMPTALERIIWGFGDGSTIPVYDT 180

Query: 181 SIGKIGAAICWENRMPLLRTAMYAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSAN 240
            +GKIGAAICWENRMPLLRTAMYAKGIEIYCAPTADSRD WQASMTHIALEGGCFVLSAN
Sbjct: 181 PLGKIGAAICWENRMPLLRTAMYAKGIEIYCAPTADSRDVWQASMTHIALEGGCFVLSAN 240

Query: 241 QFCRRKDYPPPPDYVFSGTEEELSPDSVVCAGGSVIISPSGTILAGPNYDGEALISADLD 300
           QFCRRKDYPPPP+YVFSGTEE+L+PDS+VCAGGSVIISPSG +LAGPNY GEALISADLD
Sbjct: 241 QFCRRKDYPPPPEYVFSGTEEDLTPDSIVCAGGSVIISPSGAVLAGPNYVGEALISADLD 300

Query: 301 LGDIARAKFDFDVVGHYARPEVLSLVVRDHPTTPVTFTSATTKTEDSCK 350
           LG+IARAKFDFDVVGHYARPEVLSL+VRDH  +PV+FTS ++K E S K
Sbjct: 301 LGEIARAKFDFDVVGHYARPEVLSLIVRDHAVSPVSFTSTSSKAESSPK 349

[Close]

BLAST of Carg26503 vs. Swiss-Prot
Match: sp|Q42966|NRL4B_TOBAC (Bifunctional nitrilase/nitrile hydratase NIT4B OS=Nicotiana tabacum OX=4097 GN=NIT4B PE=2 SV=1)

HSP 1 Score: 619.0 bits (1595), Expect = 3.2e-176
Identity = 298/349 (85.39%), Postives = 324/349 (92.84%), Query Frame = 0


Query: 1   MALVPTTAATDTQLIAEVDMGSDVSVPTVRATVVQASTIFYDTPATLDKAERLLAEAAGF 60
           MALVPT    +  + AEVDMG + S PTVRATVVQASTIFYDTPATLDKAERLLAEAA +
Sbjct: 1   MALVPTPVVNEGPMFAEVDMGDNSSTPTVRATVVQASTIFYDTPATLDKAERLLAEAASY 60

Query: 61  GSQLVLFPEAFVGGYPRGSNFGVSIGNRTPKGKEEFRKYHAAAIDVPGPEVDRLAAMAGK 120
           G+QLV+FPEAF+GGYPRGS FGVSIGNRT KGKEEFRKYHA+AIDVPGPEVDRLAAMAGK
Sbjct: 61  GAQLVVFPEAFIGGYPRGSTFGVSIGNRTAKGKEEFRKYHASAIDVPGPEVDRLAAMAGK 120

Query: 121 YRVHLVMGVIERDGYTLYCTVLFFDPQGRYLGKHRKVMPTALERIIWGFGDGSTIPVFET 180
           Y+V+LVMGVIERDGYTLYCTVLFFD QG YLGKHRK+MPTALERIIWGFGDGSTIPV++T
Sbjct: 121 YKVYLVMGVIERDGYTLYCTVLFFDSQGHYLGKHRKIMPTALERIIWGFGDGSTIPVYDT 180

Query: 181 SIGKIGAAICWENRMPLLRTAMYAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSAN 240
            +GKIGAAICWENRMPLLRTAMYAKGIEIYCAPTADSRD WQASMTHIALEGGCFVLSAN
Sbjct: 181 PLGKIGAAICWENRMPLLRTAMYAKGIEIYCAPTADSRDVWQASMTHIALEGGCFVLSAN 240

Query: 241 QFCRRKDYPPPPDYVFSGTEEELSPDSVVCAGGSVIISPSGTILAGPNYDGEALISADLD 300
           QFCRRKDYPPPP+YVFSGT E+L+PDS+VCAGGSVIISPSG +LAGPNY+GEALISADLD
Sbjct: 241 QFCRRKDYPPPPEYVFSGT-EDLTPDSIVCAGGSVIISPSGAVLAGPNYEGEALISADLD 300

Query: 301 LGDIARAKFDFDVVGHYARPEVLSLVVRDHPTTPVTFTSATTKTEDSCK 350
           LG+IARAKFDFDVVGHYARPEVLSL+VRDH  +PV+FTS ++K E S K
Sbjct: 301 LGEIARAKFDFDVVGHYARPEVLSLIVRDHAVSPVSFTSTSSKAESSPK 348

[Close]

BLAST of Carg26503 vs. Swiss-Prot
Match: sp|Q3LRV4|NRL4B_LUPAN (Bifunctional nitrilase/nitrile hydratase NIT4B OS=Lupinus angustifolius OX=3871 GN=NIT4B PE=1 SV=1)

HSP 1 Score: 613.6 bits (1581), Expect = 1.4e-174
Identity = 298/350 (85.14%), Postives = 322/350 (92.00%), Query Frame = 0


Query: 1   MALVPTT-AATDTQLIAEVDMGSDVSVPTVRATVVQASTIFYDTPATLDKAERLLAEAAG 60
           MALV TT    D  L AEVDM S  +  TVRATVVQASTIFYDTPATLDKAERLL +AA 
Sbjct: 1   MALVTTTPTVNDEPLFAEVDMASYFTSTTVRATVVQASTIFYDTPATLDKAERLLVQAAS 60

Query: 61  FGSQLVLFPEAFVGGYPRGSNFGVSIGNRTPKGKEEFRKYHAAAIDVPGPEVDRLAAMAG 120
           +G+Q+V+FPEAF+GGYPRGSNFGVSIGNRT KGKEEFRKYH+AAIDVPGPEVDRL+AMAG
Sbjct: 61  YGAQIVVFPEAFIGGYPRGSNFGVSIGNRTAKGKEEFRKYHSAAIDVPGPEVDRLSAMAG 120

Query: 121 KYRVHLVMGVIERDGYTLYCTVLFFDPQGRYLGKHRKVMPTALERIIWGFGDGSTIPVFE 180
           KY+V+LVMGVIERDGYTLYCTVLFFD QGRYLGKHRKVMPTALERIIWGFGDGSTIPVF+
Sbjct: 121 KYKVYLVMGVIERDGYTLYCTVLFFDSQGRYLGKHRKVMPTALERIIWGFGDGSTIPVFQ 180

Query: 181 TSIGKIGAAICWENRMPLLRTAMYAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSA 240
           T IGKIGAAICWEN+MPLLRTAMYAKG+EIYCAPTADSRD WQAS THIALEGGCFVLSA
Sbjct: 181 TPIGKIGAAICWENKMPLLRTAMYAKGVEIYCAPTADSRDLWQASTTHIALEGGCFVLSA 240

Query: 241 NQFCRRKDYPPPPDYVFSGTEEELSPDSVVCAGGSVIISPSGTILAGPNYDGEALISADL 300
           NQFCRRKDYPPPP+YVFSGTEE+L+PDSVV AGGSVIISPSG +LAGPNY+GEALISADL
Sbjct: 241 NQFCRRKDYPPPPEYVFSGTEEDLTPDSVVSAGGSVIISPSGAVLAGPNYEGEALISADL 300

Query: 301 DLGDIARAKFDFDVVGHYARPEVLSLVVRDHPTTPVTFTSATTKTEDSCK 350
           DLG+IARAKFDFDVVGHY+R EVLSL+V+DHPT PVTFTS +TK ED  K
Sbjct: 301 DLGEIARAKFDFDVVGHYSRSEVLSLIVKDHPTNPVTFTSTSTKIEDQTK 350

[Close]

BLAST of Carg26503 vs. Swiss-Prot
Match: sp|P46011|NRL4_ARATH (Bifunctional nitrilase/nitrile hydratase NIT4 OS=Arabidopsis thaliana OX=3702 GN=NIT4 PE=1 SV=1)

HSP 1 Score: 548.9 bits (1413), Expect = 4.1e-155
Identity = 266/344 (77.33%), Postives = 302/344 (87.79%), Query Frame = 0


Query: 7   TAATDT---QLIAEVDMGSDVSVPTVRATVVQASTIFYDTPATLDKAERLLAEAAGFGSQ 66
           TAA  T   Q+  E+DM +  S   VRATVVQAST+FYDTPATLDKAERLL+EAA  GSQ
Sbjct: 11  TAAPQTNGHQIFPEIDMSAGDSSSIVRATVVQASTVFYDTPATLDKAERLLSEAAENGSQ 70

Query: 67  LVLFPEAFVGGYPRGSNFGVSIGNRTPKGKEEFRKYHAAAIDVPGPEVDRLAAMAGKYRV 126
           LV+FPEAF+GGYPRGS F ++IG+RT KG+++FRKYHA+AIDVPGPEV+RLA MA KY+V
Sbjct: 71  LVVFPEAFIGGYPRGSTFELAIGSRTAKGRDDFRKYHASAIDVPGPEVERLALMAKKYKV 130

Query: 127 HLVMGVIERDGYTLYCTVLFFDPQGRYLGKHRKVMPTALERIIWGFGDGSTIPVFETSIG 186
           +LVMGVIER+GYTLYCTVLFFD QG +LGKHRK+MPTALER IWGFGDGSTIPVF+T IG
Sbjct: 131 YLVMGVIEREGYTLYCTVLFFDSQGLFLGKHRKLMPTALERCIWGFGDGSTIPVFDTPIG 190

Query: 187 KIGAAICWENRMPLLRTAMYAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSANQFC 246
           KIGAAICWENRMP LRTAMYAKGIEIYCAPTADSR+TW ASMTHIALEGGCFVLSANQFC
Sbjct: 191 KIGAAICWENRMPSLRTAMYAKGIEIYCAPTADSRETWLASMTHIALEGGCFVLSANQFC 250

Query: 247 RRKDYPPPPDYVFSGTEEELSPDSVVCAGGSVIISPSGTILAGPNYDGEALISADLDLGD 306
           RRKDYP PP+Y+FSG+EE L+PDSVVCAGGS IISP G +LAGPNY GEALI+ADLDLGD
Sbjct: 251 RRKDYPSPPEYMFSGSEESLTPDSVVCAGGSSIISPLGIVLAGPNYRGEALITADLDLGD 310

Query: 307 IARAKFDFDVVGHYARPEVLSLVVRDHPTTPVTFTSATTKTEDS 348
           IARAKFDFDVVGHY+RPEV SL +R+HP   V+F ++    ++S
Sbjct: 311 IARAKFDFDVVGHYSRPEVFSLNIREHPRKAVSFKTSKVMEDES 354

[Close]

BLAST of Carg26503 vs. TrEMBL
Match: tr|A0A0A0LVI0|A0A0A0LVI0_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G168360 PE=4 SV=1)

HSP 1 Score: 686.8 bits (1771), Expect = 2.5e-194
Identity = 336/350 (96.00%), Postives = 343/350 (98.00%), Query Frame = 0


Query: 1   MALVPTTAATDTQLIAEVDMGSDVSVPTVRATVVQASTIFYDTPATLDKAERLLAEAAGF 60
           MALVPTTA TD+QLIAEVDMGSD SV  VRATVVQASTIFYDTPATLDKAERLLAEAAGF
Sbjct: 1   MALVPTTAPTDSQLIAEVDMGSDSSVMNVRATVVQASTIFYDTPATLDKAERLLAEAAGF 60

Query: 61  GSQLVLFPEAFVGGYPRGSNFGVSIGNRTPKGKEEFRKYHAAAIDVPGPEVDRLAAMAGK 120
           GSQLV+FPEAFVGGYPRGSNFGVSIGNRTPKGKEEFRKYHA+AIDVPGPEVDRLAAMAGK
Sbjct: 61  GSQLVVFPEAFVGGYPRGSNFGVSIGNRTPKGKEEFRKYHASAIDVPGPEVDRLAAMAGK 120

Query: 121 YRVHLVMGVIERDGYTLYCTVLFFDPQGRYLGKHRKVMPTALERIIWGFGDGSTIPVFET 180
           YRVHLVMGVIERDGYTLYCTVLFFDPQGRYLGKHRKVMPTALERIIWGFGDGSTIPVFET
Sbjct: 121 YRVHLVMGVIERDGYTLYCTVLFFDPQGRYLGKHRKVMPTALERIIWGFGDGSTIPVFET 180

Query: 181 SIGKIGAAICWENRMPLLRTAMYAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSAN 240
           SIGKIGAAICWENRMPLLRTAMYAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSAN
Sbjct: 181 SIGKIGAAICWENRMPLLRTAMYAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSAN 240

Query: 241 QFCRRKDYPPPPDYVFSGTEEELSPDSVVCAGGSVIISPSGTILAGPNYDGEALISADLD 300
           QFCRRKDYPPPP+YVFSGTEEEL+PDSVVCAGGS IISPSGTILAGPNYDGEALISADLD
Sbjct: 241 QFCRRKDYPPPPEYVFSGTEEELTPDSVVCAGGSAIISPSGTILAGPNYDGEALISADLD 300

Query: 301 LGDIARAKFDFDVVGHYARPEVLSLVVRDHPTTPVTFTSATTKTEDSCKK 351
           LG+IARAKFDFDVVGHYARPEVLSLVVRDHPTTPVTFTS +TK EDSCKK
Sbjct: 301 LGEIARAKFDFDVVGHYARPEVLSLVVRDHPTTPVTFTSTSTKVEDSCKK 350

[Close]

BLAST of Carg26503 vs. TrEMBL
Match: tr|A0A1S3CLB4|A0A1S3CLB4_CUCME (bifunctional nitrilase/nitrile hydratase NIT4A OS=Cucumis melo OX=3656 GN=LOC103501743 PE=4 SV=1)

HSP 1 Score: 683.7 bits (1763), Expect = 2.2e-193
Identity = 335/350 (95.71%), Postives = 341/350 (97.43%), Query Frame = 0


Query: 1   MALVPTTAATDTQLIAEVDMGSDVSVPTVRATVVQASTIFYDTPATLDKAERLLAEAAGF 60
           MALVPTTA TD QLIAEVDMG+D SV TVRATVVQASTIFYDTPATLDKAERLLAEAAGF
Sbjct: 1   MALVPTTAPTDPQLIAEVDMGTDSSVMTVRATVVQASTIFYDTPATLDKAERLLAEAAGF 60

Query: 61  GSQLVLFPEAFVGGYPRGSNFGVSIGNRTPKGKEEFRKYHAAAIDVPGPEVDRLAAMAGK 120
           GSQLV+FPEAFVGGYPRGSNFGVSIGNRTPKGKEEFRKYHAAAIDVPGPEVDRLAAMAGK
Sbjct: 61  GSQLVVFPEAFVGGYPRGSNFGVSIGNRTPKGKEEFRKYHAAAIDVPGPEVDRLAAMAGK 120

Query: 121 YRVHLVMGVIERDGYTLYCTVLFFDPQGRYLGKHRKVMPTALERIIWGFGDGSTIPVFET 180
           YRVHLVMGVIERDGYTLYCTVLFFDPQGRYLGKHRKVMPTALERIIWGFGDGSTIPVFET
Sbjct: 121 YRVHLVMGVIERDGYTLYCTVLFFDPQGRYLGKHRKVMPTALERIIWGFGDGSTIPVFET 180

Query: 181 SIGKIGAAICWENRMPLLRTAMYAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSAN 240
           SIGKIGAAICWENRMPLLRTAMYAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSAN
Sbjct: 181 SIGKIGAAICWENRMPLLRTAMYAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSAN 240

Query: 241 QFCRRKDYPPPPDYVFSGTEEELSPDSVVCAGGSVIISPSGTILAGPNYDGEALISADLD 300
           QFCRRKDYPPPP+YVFSGTEEEL+PDSVVCAGGS IISPSGTILAGPNYDGEALISADLD
Sbjct: 241 QFCRRKDYPPPPEYVFSGTEEELTPDSVVCAGGSAIISPSGTILAGPNYDGEALISADLD 300

Query: 301 LGDIARAKFDFDVVGHYARPEVLSLVVRDHPTTPVTFTSATTKTEDSCKK 351
           LG+IARAKFDFDVVGHYARPEVLSLVVRDHP  PVTFTS +TK EDSCKK
Sbjct: 301 LGEIARAKFDFDVVGHYARPEVLSLVVRDHPAIPVTFTSTSTKAEDSCKK 350

[Close]

BLAST of Carg26503 vs. TrEMBL
Match: tr|A0A2P5CW35|A0A2P5CW35_9ROSA (Nitrilase/cyanide hydratase OS=Trema orientalis OX=63057 GN=TorNIT1 PE=4 SV=1)

HSP 1 Score: 643.3 bits (1658), Expect = 3.2e-181
Identity = 309/347 (89.05%), Postives = 330/347 (95.10%), Query Frame = 0


Query: 1   MALVPTTAATDTQLIAEVDMGSDVSVPTVRATVVQASTIFYDTPATLDKAERLLAEAAGF 60
           MALVP+    D  L AEVDMG+D S P+VRATVVQAST+FYDTPATLDKAERLLAEAAG+
Sbjct: 1   MALVPSAPLNDAPLFAEVDMGADSSAPSVRATVVQASTVFYDTPATLDKAERLLAEAAGY 60

Query: 61  GSQLVLFPEAFVGGYPRGSNFGVSIGNRTPKGKEEFRKYHAAAIDVPGPEVDRLAAMAGK 120
           GSQLV+FPEAFVGGYPRGSNFGV+IGNRTPKGKEEFRKYHA+AIDVPGPEVDRLAAMAGK
Sbjct: 61  GSQLVVFPEAFVGGYPRGSNFGVTIGNRTPKGKEEFRKYHASAIDVPGPEVDRLAAMAGK 120

Query: 121 YRVHLVMGVIERDGYTLYCTVLFFDPQGRYLGKHRKVMPTALERIIWGFGDGSTIPVFET 180
           Y+V+LVMGVIERDGYTLYCTVLFFD QGRYLGKHRKVMPTALERIIWGFGDGSTIPVFET
Sbjct: 121 YKVYLVMGVIERDGYTLYCTVLFFDSQGRYLGKHRKVMPTALERIIWGFGDGSTIPVFET 180

Query: 181 SIGKIGAAICWENRMPLLRTAMYAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSAN 240
            IGKIGAAICWENRMPLLRTAMYAKG+EIYCAPTAD+R+ WQASMTHIALEG CFVLSAN
Sbjct: 181 PIGKIGAAICWENRMPLLRTAMYAKGVEIYCAPTADAREVWQASMTHIALEGACFVLSAN 240

Query: 241 QFCRRKDYPPPPDYVFSGTEEELSPDSVVCAGGSVIISPSGTILAGPNYDGEALISADLD 300
           QFCRRKDYPPPP+YVFSGTE EL+PDSVVCAGGSVIISPSGT+LAGPNYDGEALISADLD
Sbjct: 241 QFCRRKDYPPPPEYVFSGTEGELTPDSVVCAGGSVIISPSGTVLAGPNYDGEALISADLD 300

Query: 301 LGDIARAKFDFDVVGHYARPEVLSLVVRDHPTTPVTFTSATTKTEDS 348
           LG+IARAKFDFDVVGHY+RPEVLSL+VRDHPT PVTFTS++ KTE S
Sbjct: 301 LGEIARAKFDFDVVGHYSRPEVLSLIVRDHPTNPVTFTSSSLKTESS 347

[Close]

BLAST of Carg26503 vs. TrEMBL
Match: tr|A0A2P5E021|A0A2P5E021_PARAD (Nitrilase/cyanide hydratase OS=Parasponia andersonii OX=3476 GN=PanNIT1 PE=4 SV=1)

HSP 1 Score: 638.3 bits (1645), Expect = 1.0e-179
Identity = 306/347 (88.18%), Postives = 329/347 (94.81%), Query Frame = 0


Query: 1   MALVPTTAATDTQLIAEVDMGSDVSVPTVRATVVQASTIFYDTPATLDKAERLLAEAAGF 60
           MALVP+    D  L AEVDMG+D S  TVRATVVQAST++YDTPATLDKAERLLAEAAG+
Sbjct: 1   MALVPSAPLNDAPLFAEVDMGADSSASTVRATVVQASTVYYDTPATLDKAERLLAEAAGY 60

Query: 61  GSQLVLFPEAFVGGYPRGSNFGVSIGNRTPKGKEEFRKYHAAAIDVPGPEVDRLAAMAGK 120
           GSQLV+FPEAFVGGYPRGSNFGV+IGNRTPKGKEEFRKYHA+AIDVPGPEVD LAAMAGK
Sbjct: 61  GSQLVVFPEAFVGGYPRGSNFGVTIGNRTPKGKEEFRKYHASAIDVPGPEVDLLAAMAGK 120

Query: 121 YRVHLVMGVIERDGYTLYCTVLFFDPQGRYLGKHRKVMPTALERIIWGFGDGSTIPVFET 180
           Y+V+LVMGVIERDGYTLYCTVLFFD QGRY+GKHRKVMPTALERIIWGFGDGSTIPVFET
Sbjct: 121 YKVYLVMGVIERDGYTLYCTVLFFDSQGRYMGKHRKVMPTALERIIWGFGDGSTIPVFET 180

Query: 181 SIGKIGAAICWENRMPLLRTAMYAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSAN 240
            IGKIGAAICWENRMPLLRTAMYAKG+EIYCAPTAD+R+ WQASMTHIA+EGGCFVLSAN
Sbjct: 181 PIGKIGAAICWENRMPLLRTAMYAKGVEIYCAPTADAREVWQASMTHIAVEGGCFVLSAN 240

Query: 241 QFCRRKDYPPPPDYVFSGTEEELSPDSVVCAGGSVIISPSGTILAGPNYDGEALISADLD 300
           QFCRRKDYPPPP+YVFSGTE EL+PDSVVCAGGSVIISPSGT+LAGPNYDGEALISADLD
Sbjct: 241 QFCRRKDYPPPPEYVFSGTEGELTPDSVVCAGGSVIISPSGTVLAGPNYDGEALISADLD 300

Query: 301 LGDIARAKFDFDVVGHYARPEVLSLVVRDHPTTPVTFTSATTKTEDS 348
           LG+IARAKFDFDVVGHY+RPEVLSL+VRDHPT PVTFTS++ KTE S
Sbjct: 301 LGEIARAKFDFDVVGHYSRPEVLSLIVRDHPTNPVTFTSSSLKTESS 347

[Close]

BLAST of Carg26503 vs. TrEMBL
Match: tr|A0A2P6QEB8|A0A2P6QEB8_ROSCH (Putative hydrolase, 3-cyanoalanine hydratase OS=Rosa chinensis OX=74649 GN=RchiOBHm_Chr5g0047031 PE=4 SV=1)

HSP 1 Score: 633.3 bits (1632), Expect = 3.3e-178
Identity = 308/350 (88.00%), Postives = 330/350 (94.29%), Query Frame = 0


Query: 1   MALVPTTA-ATDTQLIAEVDMGSDVSVPTVRATVVQASTIFYDTPATLDKAERLLAEAAG 60
           MALVP++A   ++ +I EVDMG D S PTVRATVVQAST+FYDTPATLDKAERLLAEAAG
Sbjct: 1   MALVPSSAPVNESPVITEVDMGGDDSTPTVRATVVQASTVFYDTPATLDKAERLLAEAAG 60

Query: 61  FGSQLVLFPEAFVGGYPRGSNFGVSIGNRTPKGKEEFRKYHAAAIDVPGPEVDRLAAMAG 120
           +GSQLV+FPEAFVGGYPRGSNFGV+IGNRT KGKEEFRKYHAAAI VPGPEVDRLA+MAG
Sbjct: 61  YGSQLVVFPEAFVGGYPRGSNFGVAIGNRTAKGKEEFRKYHAAAITVPGPEVDRLASMAG 120

Query: 121 KYRVHLVMGVIERDGYTLYCTVLFFDPQGRYLGKHRKVMPTALERIIWGFGDGSTIPVFE 180
           KY+V LVMGVIE++GYTLYCTVLFFD QG YLGKHRK+MPTALERIIWGFGDGSTIPVF+
Sbjct: 121 KYKVFLVMGVIEKEGYTLYCTVLFFDSQGCYLGKHRKIMPTALERIIWGFGDGSTIPVFD 180

Query: 181 TSIGKIGAAICWENRMPLLRTAMYAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSA 240
           T IGKIGAAICWEN+MPLLRTAMYAKGIEIYCAPTADSRD WQASMTHIALEGGCFVLSA
Sbjct: 181 TPIGKIGAAICWENKMPLLRTAMYAKGIEIYCAPTADSRDLWQASMTHIALEGGCFVLSA 240

Query: 241 NQFCRRKDYPPPPDYVFSGTEEELSPDSVVCAGGSVIISPSGTILAGPNYDGEALISADL 300
           NQFCRRKDYPPPP+Y+FSGTEEEL+PDSVVCAGGSVIISPSGTILAGPNYDGEALISADL
Sbjct: 241 NQFCRRKDYPPPPEYIFSGTEEELTPDSVVCAGGSVIISPSGTILAGPNYDGEALISADL 300

Query: 301 DLGDIARAKFDFDVVGHYARPEVLSLVVRDHPTTPVTFTSATTKTEDSCK 350
           D+GDIARAKFDFDVVGHYARPEVLSL+VRDHP  PVTFTSA+ KTE S K
Sbjct: 301 DIGDIARAKFDFDVVGHYARPEVLSLIVRDHPANPVTFTSASAKTEVSDK 350

The following BLAST results are available for this feature:

BLAST of Carg26503 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023520715.1	1.2e-200	99.43	bifunctional nitrilase/nitrile hydratase NIT4A [Cucurbita pepo subsp. pepo]	[more]
XP_022927562.1	2.3e-199	99.14	bifunctional nitrilase/nitrile hydratase NIT4A [Cucurbita moschata]	[more]
XP_022973146.1	8.8e-199	98.57	bifunctional nitrilase/nitrile hydratase NIT4A [Cucurbita maxima]	[more]
XP_004139500.1	3.8e-194	96.00	PREDICTED: bifunctional nitrilase/nitrile hydratase NIT4A [Cucumis sativus] >KGN...	[more]
XP_008463648.1	3.3e-193	95.71	PREDICTED: bifunctional nitrilase/nitrile hydratase NIT4A [Cucumis melo]	[more]

BLAST of Carg26503 vs. TAIR10


Match Name	E-value	Identity	Description
AT5G22300.1	2.3e-156	77.33	nitrilase 4	[more]
AT3G44300.1	6.0e-133	67.39	nitrilase 2	[more]
AT3G44320.1	7.9e-133	67.18	nitrilase 3	[more]
AT3G44310.1	6.9e-129	65.33	nitrilase 1	[more]
AT2G27450.2	8.7e-15	25.95	nitrilase-like protein 1	[more]

BLAST of Carg26503 vs. Swiss-Prot


Match Name	E-value	Identity	Description
sp\|Q5QGZ8\|NRL4A_LUPAN	7.7e-178	85.67	Bifunctional nitrilase/nitrile hydratase NIT4A OS=Lupinus angustifolius OX=3871 ...	[more]
sp\|Q42965\|NRL4A_TOBAC	8.5e-177	85.67	Bifunctional nitrilase/nitrile hydratase NIT4A OS=Nicotiana tabacum OX=4097 GN=N...	[more]
sp\|Q42966\|NRL4B_TOBAC	3.2e-176	85.39	Bifunctional nitrilase/nitrile hydratase NIT4B OS=Nicotiana tabacum OX=4097 GN=N...	[more]
sp\|Q3LRV4\|NRL4B_LUPAN	1.4e-174	85.14	Bifunctional nitrilase/nitrile hydratase NIT4B OS=Lupinus angustifolius OX=3871 ...	[more]
sp\|P46011\|NRL4_ARATH	4.1e-155	77.33	Bifunctional nitrilase/nitrile hydratase NIT4 OS=Arabidopsis thaliana OX=3702 GN...	[more]

BLAST of Carg26503 vs. TrEMBL


Match Name	E-value	Identity	Description
tr\|A0A0A0LVI0\|A0A0A0LVI0_CUCSA	2.5e-194	96.00	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G168360 PE=4 SV=1	[more]
tr\|A0A1S3CLB4\|A0A1S3CLB4_CUCME	2.2e-193	95.71	bifunctional nitrilase/nitrile hydratase NIT4A OS=Cucumis melo OX=3656 GN=LOC103...	[more]
tr\|A0A2P5CW35\|A0A2P5CW35_9ROSA	3.2e-181	89.05	Nitrilase/cyanide hydratase OS=Trema orientalis OX=63057 GN=TorNIT1 PE=4 SV=1	[more]
tr\|A0A2P5E021\|A0A2P5E021_PARAD	1.0e-179	88.18	Nitrilase/cyanide hydratase OS=Parasponia andersonii OX=3476 GN=PanNIT1 PE=4 SV=...	[more]
tr\|A0A2P6QEB8\|A0A2P6QEB8_ROSCH	3.3e-178	88.00	Putative hydrolase, 3-cyanoalanine hydratase OS=Rosa chinensis OX=74649 GN=RchiO...	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **Molecular Function**
Term	Definition
GO:0016810	hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds

Vocabulary: **Biological Process**
Term	Definition
GO:0006807	nitrogen compound metabolic process

Vocabulary: **INTERPRO**
Term	Definition
IPR000132	Nitrilase/CN_hydratase_CS
IPR003010	C-N_Hydrolase
IPR036526	C-N_Hydrolase_sf

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006807	nitrogen compound metabolic process
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0016810	hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg26503-RA	Carg26503-RA	mRNA

InterPro

Analysis Name: InterPro Annotations of silver-seed gourd
Date Performed: 2019-03-07


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR036526	Carbon-nitrogen hydrolase superfamily	GENE3D	G3DSA:3.60.110.10		coord: 22..313 e-value: 3.1E-104 score: 349.9
IPR036526	Carbon-nitrogen hydrolase superfamily	SUPERFAMILY	SSF56317	Carbon-nitrogen hydrolase	coord: 27..327
IPR003010	Carbon-nitrogen hydrolase	PFAM	PF00795	CN_hydrolase	coord: 30..308 e-value: 1.1E-68 score: 231.4
IPR003010	Carbon-nitrogen hydrolase	PROSITE	PS50263	CN_HYDROLASE	coord: 29..301 score: 57.436
None	No IPR available	PANTHER	PTHR23088:SF31	SUBFAMILY NOT NAMED	coord: 18..344
None	No IPR available	PANTHER	PTHR23088	NITRILASE-RELATED	coord: 18..344
None	No IPR available	CDD	cd07564	nitrilases_CHs	coord: 29..329 e-value: 6.55947E-160 score: 451.942
IPR000132	Nitrilase/cyanide hydratase, conserved site	PROSITE	PS00921	NITRIL_CHT_2	coord: 186..199
IPR000132	Nitrilase/cyanide hydratase, conserved site	PROSITE	PS00920	NITRIL_CHT_1	coord: 61..76

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Carg26503	Csa1G168360	Cucumber (Chinese Long) v2	carcuB0998
Carg26503	ClCG01G011750	Watermelon (Charleston Gray)	carwcgB0960
Carg26503	CSPI01G15000	Wild cucumber (PI 183967)	carcpiB1021
Carg26503	Cucsa.319690	Cucumber (Gy14) v1	carcgyB1102
Carg26503	CmaCh13G003490	Cucurbita maxima (Rimu)	carcmaB1362
Carg26503	CmoCh13G003800	Cucurbita moschata (Rifu)	carcmoB1323
Carg26503	Cp4.1LG00g01690	Cucurbita pepo (Zucchini)	carcpeB1370
Carg26503	CsGy1G014790	Cucumber (Gy14) v2	carcgybB0971
Carg26503	MELO3C025585.2	Melon (DHL92) v3.6.1	carmedB0967
Carg26503	CsaV3_1G015110	Cucumber (Chinese Long) v3	carcucB1027
Carg26503	Cla97C01G011330	Watermelon (97103) v2	carwmbB1037
Carg26503	Bhi08G000022	Wax gourd	carwgoB1154

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:

None