Csa7G397050 (gene) Cucumber (Chinese Long) v2 | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Csa7G397050
Type	gene
Organism	Cucumis. sativus (Cucumber (Chinese Long) v2)
Description	Pectinesterase/pectinesterase inhibitor; contains IPR006501 (Pectinesterase inhibitor)
Location	Chr7 : 15314527 .. 15315598 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CAAGATTTCCTTTATTGTAAAGTTCATGAGAACTCACCTGCCATTCACATGTTCTTCACCTTCTTAACTTATTTCACTTCATCACTACTTCTCCTCCCCTTCTTCAATGCATTCACCATCTCCACGCCTAATTTATAGCCAATACCTGTAAAAAGTTATCCAAAATGAACTTTTCAAGTCACCCCAATACCCGTTTCCCCACTATATAATCCACCCATAATCTCATTAATCCCCACATTAGTCTCAATCTCATTCACAAATCACCCCACTATATACTGATTTCATAGAAATTTATATTCTATCCCCACATAACTTTTTCACTTCTCTCCGGCATTTCATCAAACATGTTGCGTGCACCCAGTAGAAGAGCATCAATCATAGCCCTTTTGGCCCTCATCTCCATTCTCCCATGGCTGACCCACTCCGCCAAAACGAGCTATGTCCAAGAAGCCTGCAGGGTAACACGACACCAAGATCTCTGCATTCAATCCCTGTCCCCATTTTCAAGCGCCGCCAAGCGCAGCCCAACAAAATGGGCTCGAGCGGGGGTGTCGGTGACAATAACAGAGGCAAAAAAAGTAGCAGGACTCTTGGGGAGGTTGAAGAACAACAAGCGGATGAAGGGGAGAAACAGGGCAGCAGTTTTGGACTGTGTGGAGGTGTTTGAAGCCGCCATTGATGAGCTTCACAGGTCCCTTGGGGTTCTTAGAAGATTGAGTAGAAGGAATTTTGACGCACAAATGGGGGATCTAACAACTTGGGTCAGCGCTGCACTGACCGATGAAGATACTTGCGTTGAAGGATTTGAAGGTGAAGAGGGAAAAGTAGTGACTTTGCTAAGGAATCGAGTGGTGAAAGTTGGTTATATTACGAGCAATGCACTTGCTTTGGTTAACAAATTGGCAGCCTCTAGTTTTGAAACTACAATAAATATGTAAGGAGATTTGAAATTTCTCTTGAATGGAGATTGGATTTACATGTTATAAGATTGTATGATTTGTAATTGTACTAAAGAGGGGTTGAGTTTGGAATGAGGGGTTTTGACATTTGAAGTCGATCCATCTTTGAACAC

mRNA sequence

ATGTTGCGTGCACCCAGTAGAAGAGCATCAATCATAGCCCTTTTGGCCCTCATCTCCATTCTCCCATGGCTGACCCACTCCGCCAAAACGAGCTATGTCCAAGAAGCCTGCAGGGTAACACGACACCAAGATCTCTGCATTCAATCCCTGTCCCCATTTTCAAGCGCCGCCAAGCGCAGCCCAACAAAATGGGCTCGAGCGGGGGTGTCGGTGACAATAACAGAGGCAAAAAAAGTAGCAGGACTCTTGGGGAGGTTGAAGAACAACAAGCGGATGAAGGGGAGAAACAGGGCAGCAGTTTTGGACTGTGTGGAGGTGTTTGAAGCCGCCATTGATGAGCTTCACAGGTCCCTTGGGGTTCTTAGAAGATTGAGTAGAAGGAATTTTGACGCACAAATGGGGGATCTAACAACTTGGGTCAGCGCTGCACTGACCGATGAAGATACTTGCGTTGAAGGATTTGAAGGTGAAGAGGGAAAAGTAGTGACTTTGCTAAGGAATCGAGTGGTGAAAGTTGGTTATATTACGAGCAATGCACTTGCTTTGGTTAACAAATTGGCAGCCTCTAGTTTTGAAACTACAATAAATATGTAA

Coding sequence (CDS)

ATGTTGCGTGCACCCAGTAGAAGAGCATCAATCATAGCCCTTTTGGCCCTCATCTCCATTCTCCCATGGCTGACCCACTCCGCCAAAACGAGCTATGTCCAAGAAGCCTGCAGGGTAACACGACACCAAGATCTCTGCATTCAATCCCTGTCCCCATTTTCAAGCGCCGCCAAGCGCAGCCCAACAAAATGGGCTCGAGCGGGGGTGTCGGTGACAATAACAGAGGCAAAAAAAGTAGCAGGACTCTTGGGGAGGTTGAAGAACAACAAGCGGATGAAGGGGAGAAACAGGGCAGCAGTTTTGGACTGTGTGGAGGTGTTTGAAGCCGCCATTGATGAGCTTCACAGGTCCCTTGGGGTTCTTAGAAGATTGAGTAGAAGGAATTTTGACGCACAAATGGGGGATCTAACAACTTGGGTCAGCGCTGCACTGACCGATGAAGATACTTGCGTTGAAGGATTTGAAGGTGAAGAGGGAAAAGTAGTGACTTTGCTAAGGAATCGAGTGGTGAAAGTTGGTTATATTACGAGCAATGCACTTGCTTTGGTTAACAAATTGGCAGCCTCTAGTTTTGAAACTACAATAAATATGTAA

Protein sequence

MLRAPSRRASIIALLALISILPWLTHSAKTSYVQEACRVTRHQDLCIQSLSPFSSAAKRSPTKWARAGVSVTITEAKKVAGLLGRLKNNKRMKGRNRAAVLDCVEVFEAAIDELHRSLGVLRRLSRRNFDAQMGDLTTWVSAALTDEDTCVEGFEGEEGKVVTLLRNRVVKVGYITSNALALVNKLAASSFETTINM*

Homology

[Close]

BLAST of Csa7G397050 vs. Swiss-Prot
Match: 21KD_DAUCA (21 kDa protein OS=Daucus carota PE=2 SV=1)

HSP 1 Score: 120.2 bits (300), Expect = 2.6e-26
Identity = 66/184 (35.87%), Postives = 106/184 (57.61%), Query Frame = 1


Query: 10  SIIALLALISILPWLTHSAKTSYVQEACRVTRHQDLCIQSLSPFSSAAKRSPTKWARAGV 69
           S +   AL+ IL   + +    +++ +C +T +  +C QSLS ++   + +P + A   +
Sbjct: 6   STLVFSALLVILAAASAAPANQFIKTSCTLTTYPAVCEQSLSAYAKTIQNNPQELASTAL 65

Query: 70  SVTITEAKKVAGLLGRLKNNKRMKGRNRAAVLDCVEVFEAAIDELHRSLGVLRRLSR--- 129
            V++T  ++    + RL   K +K R  AA+ DC+E  E ++D + RS   ++ LS    
Sbjct: 66  QVSLTRTQQAQTFMKRLNKFKGLKARQYAAIHDCLEEVEDSLDRVSRSCDEMKNLSHAKG 125

Query: 130 RNFDAQMGDLTTWVSAALTDEDTCVEGFEGE--EGKVVTLLRNRVVKVGYITSNALALVN 189
            +F  +M ++ TWVSAALTDE TC++GF G+  +GK+   +R +VV V  +TSNALALVN
Sbjct: 126 NDFTFRMSNVETWVSAALTDETTCMDGFAGKGMDGKIKESVRAQVVAVARVTSNALALVN 185

[Close]

BLAST of Csa7G397050 vs. Swiss-Prot
Match: PME3_ARATH (Pectinesterase/pectinesterase inhibitor 3 OS=Arabidopsis thaliana GN=PME3 PE=2 SV=2)

HSP 1 Score: 73.6 bits (179), Expect = 2.8e-12
Identity = 44/158 (27.85%), Postives = 82/158 (51.90%), Query Frame = 1


Query: 33  VQEACRVTRHQDLCIQSLSPFSSAAKRSPTKWARAGVSVTITEAKKVAGLLGRL-KNNKR 92
           ++ +C  TR+ +LCI ++         S      A V++TIT  +     + +L K  K 
Sbjct: 59  LRSSCSSTRYPELCISAVVTAGGVELTSQKDVIEASVNLTITAVEHNYFTVKKLIKKRKG 118

Query: 93  MKGRNRAAVLDCVEVFEAAIDELHRSLGVLRRL-SRRNFDAQMGDLTTWVSAALTDEDTC 152
           +  R + A+ DC+E  +  +DELH ++  L    +++      GDL T +S+A+T+++TC
Sbjct: 119 LTPREKTALHDCLETIDETLDELHETVEDLHLYPTKKTLREHAGDLKTLISSAITNQETC 178

Query: 153 VEGFEGEEG--KVVTLLRNRVVKVGYITSNALALVNKL 187
           ++GF  ++   +V   L    + V ++ SNALA++  +
Sbjct: 179 LDGFSHDDADKQVRKALLKGQIHVEHMCSNALAMIKNM 216

[Close]

BLAST of Csa7G397050 vs. Swiss-Prot
Match: PME1_CITSI (Pectinesterase 1 OS=Citrus sinensis GN=PECS-1.1 PE=2 SV=1)

HSP 1 Score: 71.6 bits (174), Expect = 1.1e-11
Identity = 45/159 (28.30%), Postives = 82/159 (51.57%), Query Frame = 1


Query: 33  VQEACRVTRHQDLCIQSLSPFSSAAKRSPTKWARAGVSVTITEAKKVAGLLGRLKNNKR- 92
           ++ +C  TR+ DLC  +++    A+K+  ++     +S+ IT         G  K  KR 
Sbjct: 69  LKSSCSSTRYPDLCFSAIAAVPEASKKVTSQKDVIEMSLNITTTAVEHNYFGIQKLLKRT 128

Query: 93  -MKGRNRAAVLDCVEVFEAAIDELHRSLGVLRRL-SRRNFDAQMGDLTTWVSAALTDEDT 152
            +  R + A+ DC+E  +  +DELH+++  L    ++++      DL T +SAA+T++ T
Sbjct: 129 NLTKREKVALHDCLETIDETLDELHKAVEDLEEYPNKKSLSQHADDLKTLMSAAMTNQGT 188

Query: 153 CVEGFEGEEGK--VVTLLRNRVVKVGYITSNALALVNKL 187
           C++GF  ++    V   L +  V V  + SNALA++  +
Sbjct: 189 CLDGFSHDDANKHVRDALSDGQVHVEKMCSNALAMIKNM 227

[Close]

BLAST of Csa7G397050 vs. Swiss-Prot
Match: PME3_CITSI (Pectinesterase 3 OS=Citrus sinensis PE=1 SV=1)

HSP 1 Score: 71.6 bits (174), Expect = 1.1e-11
Identity = 45/159 (28.30%), Postives = 82/159 (51.57%), Query Frame = 1


Query: 33  VQEACRVTRHQDLCIQSLSPFSSAAKRSPTKWARAGVSVTITEAKKVAGLLGRLKNNKR- 92
           ++ +C  TR+ DLC  +++    A+K+  ++     +S+ IT         G  K  KR 
Sbjct: 69  LKSSCSSTRYPDLCFSAIAAVPEASKKVTSQKDVIEMSLNITTTAVEHNYFGIQKLLKRT 128

Query: 93  -MKGRNRAAVLDCVEVFEAAIDELHRSLGVLRRL-SRRNFDAQMGDLTTWVSAALTDEDT 152
            +  R + A+ DC+E  +  +DELH+++  L    ++++      DL T +SAA+T++ T
Sbjct: 129 NLTKREKVALHDCLETIDETLDELHKAVEDLEEYPNKKSLSQHADDLKTLMSAAMTNQGT 188

Query: 153 CVEGFEGEEGK--VVTLLRNRVVKVGYITSNALALVNKL 187
           C++GF  ++    V   L +  V V  + SNALA++  +
Sbjct: 189 CLDGFSHDDANKHVRDALSDGQVHVEKMCSNALAMIKNM 227

[Close]

BLAST of Csa7G397050 vs. Swiss-Prot
Match: PME34_ARATH (Probable pectinesterase/pectinesterase inhibitor 34 OS=Arabidopsis thaliana GN=PME34 PE=2 SV=1)

HSP 1 Score: 71.2 bits (173), Expect = 1.4e-11
Identity = 54/176 (30.68%), Postives = 86/176 (48.86%), Query Frame = 1


Query: 9   ASIIALLALISILPWLTHSAKTSYVQEACRVTRHQDLCIQSLSPFSSAAKRSPTK-WARA 68
           A + + L L   +P L      + + +AC +TR  +LC+ SL  F  +   S +K     
Sbjct: 64  AGVRSRLKLNQSVPGLARKPSQA-ISKACELTRFPELCVDSLMDFPGSLAASSSKDLIHV 123

Query: 69  GVSVTITEAKKVAGLLGRLKNNKRMKGRNRAAVLDCVEVFEAAIDELHRSLGVLRRLSRR 128
            V++T+            L +   M  R R+A   CVE+ + ++D L R+L  +   S  
Sbjct: 124 TVNMTLHHFSHALYSSASL-SFVDMPPRARSAYDSCVELLDDSVDALSRALSSVVSSS-- 183

Query: 129 NFDAQMGDLTTWVSAALTDEDTCVEGFEG-EEGKVVTLLRNRVVKVGYITSNALAL 183
              A+  D+TTW+SAALT+ DTC EGF+G ++G V   +   +  +  + SN LA+
Sbjct: 184 ---AKPQDVTTWLSAALTNHDTCTEGFDGVDDGGVKDHMTAALQNLSELVSNCLAI 232

[Close]

BLAST of Csa7G397050 vs. TrEMBL
Match: A0A0A0KAX0_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_7G397050 PE=4 SV=1)

HSP 1 Score: 382.5 bits (981), Expect = 3.1e-103
Identity = 197/197 (100.00%), Postives = 197/197 (100.00%), Query Frame = 1


Query: 1   MLRAPSRRASIIALLALISILPWLTHSAKTSYVQEACRVTRHQDLCIQSLSPFSSAAKRS 60
           MLRAPSRRASIIALLALISILPWLTHSAKTSYVQEACRVTRHQDLCIQSLSPFSSAAKRS
Sbjct: 1   MLRAPSRRASIIALLALISILPWLTHSAKTSYVQEACRVTRHQDLCIQSLSPFSSAAKRS 60

Query: 61  PTKWARAGVSVTITEAKKVAGLLGRLKNNKRMKGRNRAAVLDCVEVFEAAIDELHRSLGV 120
           PTKWARAGVSVTITEAKKVAGLLGRLKNNKRMKGRNRAAVLDCVEVFEAAIDELHRSLGV
Sbjct: 61  PTKWARAGVSVTITEAKKVAGLLGRLKNNKRMKGRNRAAVLDCVEVFEAAIDELHRSLGV 120

Query: 121 LRRLSRRNFDAQMGDLTTWVSAALTDEDTCVEGFEGEEGKVVTLLRNRVVKVGYITSNAL 180
           LRRLSRRNFDAQMGDLTTWVSAALTDEDTCVEGFEGEEGKVVTLLRNRVVKVGYITSNAL
Sbjct: 121 LRRLSRRNFDAQMGDLTTWVSAALTDEDTCVEGFEGEEGKVVTLLRNRVVKVGYITSNAL 180

Query: 181 ALVNKLAASSFETTINM 198
           ALVNKLAASSFETTINM
Sbjct: 181 ALVNKLAASSFETTINM 197

[Close]

BLAST of Csa7G397050 vs. TrEMBL
Match: I3SDE4_LOTJA (Uncharacterized protein OS=Lotus japonicus PE=2 SV=1)

HSP 1 Score: 217.6 bits (553), Expect = 1.3e-53
Identity = 115/190 (60.53%), Postives = 140/190 (73.68%), Query Frame = 1


Query: 12  IALLALISILPWLTHSAKT-----------SYVQEACRVTRHQDLCIQSLSPFSSAAKRS 71
           + LL +  +LP L ++ +            S V+EAC VTR+Q+LCI +LS FS+ A  S
Sbjct: 4   LMLLLITLLLPLLANTGQNQMVFAKGKNNNSNVREACSVTRYQNLCIHTLSHFSNTAGTS 63

Query: 72  PTKWARAGVSVTITEAKKVAGLLGRLKNNKRM-KGRNRAAVLDCVEVFEAAIDELHRSLG 131
           P+KWARAGVSVTITE K V   L +LK NKRM KGRNRAA+ DCVE F  A+DELHRSLG
Sbjct: 64  PSKWARAGVSVTITEVKNVQAYLTKLKKNKRMMKGRNRAALSDCVECFADALDELHRSLG 123

Query: 132 VLRRLSRRNFDAQMGDLTTWVSAALTDEDTCVEGFEGEEGKVVTLLRNRVVKVGYITSNA 190
           VLRRLS++ F  QMGDL TW+SAALTDEDTC++GFEG+  + + LL+NRV  V YITSNA
Sbjct: 124 VLRRLSKKTFSTQMGDLNTWISAALTDEDTCIDGFEGKTERQIKLLQNRVQNVSYITSNA 183

[Close]

BLAST of Csa7G397050 vs. TrEMBL
Match: A0A0D2T616_GOSRA (Uncharacterized protein OS=Gossypium raimondii GN=B456_008G145300 PE=4 SV=1)

HSP 1 Score: 215.3 bits (547), Expect = 6.6e-53
Identity = 105/186 (56.45%), Postives = 139/186 (74.73%), Query Frame = 1


Query: 12  IALLALISILPWL--THSAKTSYVQEACRVTRHQDLCIQSLSPFSSAAKRSPTKWARAGV 71
           + LL L+S+L W+  T  + T+YVQ+AC VTR++ LCI +L+PFSS AK SP+KWARAGV
Sbjct: 10  LTLLLLLSVLKWVQSTPHSSTNYVQDACSVTRYRALCINTLAPFSSTAKTSPSKWARAGV 69

Query: 72  SVTITEAKKVAGLLGRLKNNKRMKGRNRAAVLDCVEVFEAAIDELHRSLGVLRRLSRRNF 131
           S+TI E KKV   L ++KN + MKG  +  + DC+E  + AID+LH SLGVLR+L+ RNF
Sbjct: 70  SITIGETKKVTQYLMKVKNYRTMKGSYKIPLSDCIECLQDAIDQLHGSLGVLRKLNARNF 129

Query: 132 DAQMGDLTTWVSAALTDEDTCVEGFEGEEGKVVTLLRNRVVKVGYITSNALALVNKLAAS 191
            AQMGD+TTW+SA LTD +TC++GFE   GK   ++RNRV++  Y TSNALALVNKLA S
Sbjct: 130 YAQMGDITTWLSAVLTDHETCLDGFENPRGKQAKMVRNRVLRSSYFTSNALALVNKLATS 189

Query: 192 SFETTI 196
             + T+
Sbjct: 190 GLDNTV 195

[Close]

BLAST of Csa7G397050 vs. TrEMBL
Match: G7LBZ5_MEDTR (Plant invertase/pectin methylesterase inhibitor OS=Medicago truncatula GN=MTR_7g108790 PE=4 SV=1)

HSP 1 Score: 213.0 bits (541), Expect = 3.3e-52
Identity = 109/193 (56.48%), Postives = 141/193 (73.06%), Query Frame = 1


Query: 11  IIALLALISILPWLTHSAKTSY-----------VQEACRVTRHQDLCIQSLSPFSSAAKR 70
           +I  + LI +L +L+ S  TS            V+EAC VTR+Q+LCI +L+ FS+ A R
Sbjct: 1   MIITIRLILLLVFLSLSTNTSQIVYAKGNNNNNVKEACSVTRYQNLCIHTLAQFSNTAGR 60

Query: 71  SPTKWARAGVSVTITEAKKVAGLLGRLKNNKRMKGRNRAAVLDCVEVFEAAIDELHRSLG 130
           +P+KWARAGVSVTI+E K V   L +LK N +MKGRNR A+ DC+E F  A+DELH+SLG
Sbjct: 61  TPSKWARAGVSVTISEVKNVQAYLTKLKKNGKMKGRNRVALSDCIETFGYAVDELHKSLG 120

Query: 131 VLRRLSRRNFDAQMGDLTTWVSAALTDEDTCVEGFEGEEGKVVTLLRNRVVKVGYITSNA 190
           VLR+LS+  F  QMGDL TW+SAALT+EDTC++GFEG+  K + LL+N+V  V YITSNA
Sbjct: 121 VLRKLSKNTFSTQMGDLNTWISAALTNEDTCLDGFEGKTEKKIKLLQNKVKNVSYITSNA 180

Query: 191 LALVNKLAASSFE 193
           LALVNKLA++  E
Sbjct: 181 LALVNKLASTGLE 193

[Close]

BLAST of Csa7G397050 vs. TrEMBL
Match: B9I9R5_POPTR (Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0014s12390g PE=4 SV=1)

HSP 1 Score: 212.6 bits (540), Expect = 4.3e-52
Identity = 108/184 (58.70%), Postives = 135/184 (73.37%), Query Frame = 1


Query: 12  IALLALISILPWLTHSAKT------SYVQEACRVTRHQDLCIQSLSPFSSAAKRSPTKWA 71
           I++L     L WLT +         +YV+EAC VTR+ DLC+ SL+ FS  A RSP+KWA
Sbjct: 4   ISILITFLFLSWLTCATSRGSDNGDTYVREACSVTRYHDLCMHSLASFSHTAGRSPSKWA 63

Query: 72  RAGVSVTITEAKKVAGLLGRLKNNKRMKGRNRAAVLDCVEVFEAAIDELHRSLGVLRRLS 131
           RAGVSVTI EAK  +  L +LK ++ M+GRNR A+ DC+E F+ AID LH+SLG+LR+L 
Sbjct: 64  RAGVSVTIGEAKNASQYLNKLKKDRIMRGRNRIALSDCIECFQDAIDNLHKSLGILRKLD 123

Query: 132 RRNFDAQMGDLTTWVSAALTDEDTCVEGFEGEEGKVVTLLRNRVVKVGYITSNALALVNK 190
             NFD QMGDLTTW+SAALTDEDTC++GFE    K V +L N+V +V YITSNALALVNK
Sbjct: 124 ATNFDTQMGDLTTWLSAALTDEDTCLDGFEDRSSKQVKMLLNQVSRVTYITSNALALVNK 183

[Close]

BLAST of Csa7G397050 vs. TAIR10
Match: AT3G62820.1 (AT3G62820.1 Plant invertase/pectin methylesterase inhibitor superfamily protein)

HSP 1 Score: 158.3 bits (399), Expect = 4.9e-39
Identity = 86/167 (51.50%), Postives = 111/167 (66.47%), Query Frame = 1


Query: 27  SAKTSYVQEACRVTRHQDLCIQSLSPFSSAAKRSPTKWARAGVSVTITEAKKVAGLLGRL 86
           S + SYVQ AC VTR+QDLC ++L PF+S AK SP+KWARAGVSV IT+ K V  L   L
Sbjct: 28  SKRESYVQNACSVTRYQDLCAKTLLPFASVAKNSPSKWARAGVSVAITDNKDV--LRHLL 87

Query: 87  KNNKRMKG-RNRAAVLDCVEVFEAAIDELHRSLGVLRRLSRRNFDAQMGDLTTWVSAALT 146
           K      G R+R A+ DC E+ + ++D LH+SL VLR L    F  QM DL TW+S++LT
Sbjct: 88  KTRLSTIGKRDRIALSDCRELLQDSLDSLHKSLAVLRTLRASEFQQQMSDLATWLSSSLT 147

Query: 147 DEDTCVEGFE---GEEGKVVTLLRNRVVKVGYITSNALALVNKLAAS 190
           D+DTC++GFE         V ++R RV    Y++SN+LAL+NKLAA+
Sbjct: 148 DKDTCLDGFEKTSTRSSSTVRMIRKRVTTSMYLSSNSLALLNKLAAN 192

[Close]

BLAST of Csa7G397050 vs. TAIR10
Match: AT2G47670.1 (AT2G47670.1 Plant invertase/pectin methylesterase inhibitor superfamily protein)

HSP 1 Score: 139.8 bits (351), Expect = 1.8e-33
Identity = 74/161 (45.96%), Postives = 107/161 (66.46%), Query Frame = 1


Query: 30  TSYVQEACRVTRHQDLCIQSLSPFSSAAKRSPTKWARAGVSVTITEAKKVAGLLGRLKNN 89
           ++YV+ AC VTR+  LC+++L PF+  A+ + +KWARA V+VTIT+ K+V  LL  LK  
Sbjct: 46  STYVRNACNVTRYNRLCVRTLWPFAIVARNNTSKWARASVAVTITDTKRVLRLL--LKTQ 105

Query: 90  KRMKGRN-RAAVLDCVEVFEAAIDELHRSLGVLRRLSRRNFDAQMGDLTTWVSAALTDED 149
           +   G + R A+ DC E+F  ++D L++SL VLR L+   F  Q+ DL TW+SAALTD+D
Sbjct: 106 RSAVGESERIALSDCRELFVDSLDNLYKSLAVLRTLNADEFQRQISDLATWLSAALTDDD 165

Query: 150 TCVEGFE--GEEGKVVTLLRNRVVKVGYITSNALALVNKLA 188
           TC++GFE      + V ++R +  K   + SNALAL+ KLA
Sbjct: 166 TCLDGFEETSSRTRTVRMVRRKATKCMRLCSNALALLKKLA 204

[Close]

BLAST of Csa7G397050 vs. TAIR10
Match: AT5G62360.1 (AT5G62360.1 Plant invertase/pectin methylesterase inhibitor superfamily protein)

HSP 1 Score: 135.2 bits (339), Expect = 4.4e-32
Identity = 72/182 (39.56%), Postives = 114/182 (62.64%), Query Frame = 1


Query: 11  IIALLALI--SILPWLTHSAKTSYVQEACRVTRHQDLCIQSLSPFSSAAKRSPTKWARAG 70
           IIA+L L+  +     T +  T +V+ +C  T +  LC  SLS  +S  + SP   A A 
Sbjct: 20  IIAMLKLVHTTTTTTTTTTTNTEFVKSSCTFTTYPRLCFSSLSTHASLIQTSPKLMAHAA 79

Query: 71  VSVTITEAKKVAGLLGRLKNNKRMKGRNRAAVLDCVEVFEAAIDELHRSLGVLRRLSRRN 130
           +++T+  AK  + ++ RL N+ R+K +  +A+ DCVE     ++EL +S+G + +LS  N
Sbjct: 80  LNITLASAKVTSAMMVRLSNS-RLKPKEVSAMRDCVEELGDTLEELRKSIGEMCQLSGSN 139

Query: 131 FDAQMGDLTTWVSAALTDEDTCVEGFEGE--EGKVVTLLRNRVVKVGYITSNALALVNKL 189
           ++  + D+ TWVSAALTD +TC +GFEGE  +GKV  L+R R++ + ++TSNALAL+N  
Sbjct: 140 YEVYISDIQTWVSAALTDVNTCTDGFEGEDMDGKVKVLVRGRILVIAHLTSNALALINHF 199

[Close]

BLAST of Csa7G397050 vs. TAIR10
Match: AT4G12390.1 (AT4G12390.1 pectin methylesterase inhibitor 1)

HSP 1 Score: 121.3 bits (303), Expect = 6.6e-28
Identity = 64/169 (37.87%), Postives = 104/169 (61.54%), Query Frame = 1


Query: 27  SAKTSYVQEACRVTRHQDLCIQSLSPFSSAAKRSPTKWARAGVSVTITEAKKVAGLLGRL 86
           S+  +++  +CRVTR+Q LC++ L+ F+   +R+  + A+  ++VT+   +     +G+L
Sbjct: 34  SSSINFIVSSCRVTRYQTLCVKCLAAFADKIRRNENQLAQTALAVTLVRVQSTTIYVGKL 93

Query: 87  KNNKRMKGRNRAAVLDCVEVFEAAIDELHRSLGVLRRLSRRNFDA-----QMGDLTTWVS 146
              +R+K R   AV DCVE     ++ L +S+  L+++ R   D      ++ ++ TWVS
Sbjct: 94  TKARRIKRREYLAVKDCVENLGDGLEMLAQSMRELKQVGRSGRDRDEFLWRLSNVETWVS 153

Query: 147 AALTDEDTCVEGFEGE--EGKVVTLLRNRVVKVGYITSNALALVNKLAA 189
           AALTDE TC++GF+G+  +G V + +R RVV V  +TSNALALVN+ AA
Sbjct: 154 AALTDETTCLDGFDGKVMDGVVKSAIRRRVVHVARVTSNALALVNRFAA 202

[Close]

BLAST of Csa7G397050 vs. TAIR10
Match: AT5G20740.1 (AT5G20740.1 Plant invertase/pectin methylesterase inhibitor superfamily protein)

HSP 1 Score: 115.2 bits (287), Expect = 4.7e-26
Identity = 59/167 (35.33%), Postives = 102/167 (61.08%), Query Frame = 1


Query: 26  HSAKTSYVQEACRVTRHQDLCIQSLSPFSSAAKRSPTKWARAGVSVTITEAKKVAGLLGR 85
           H+     V  +C    +  LC+++LS +S     +    A+A + ++++ A+  A  L  
Sbjct: 27  HNGAEDIVHSSCEHASYPSLCVRTLSSYSGPTITNRRDLAQAAIKISLSHAQSAAKKLAV 86

Query: 86  LKNNKRMKGRNRAAVLDCVEVFEAAIDELHRSLGVLRRL-----SRRNFDAQMGDLTTWV 145
           ++++   K + +AA++DCVE+   ++DEL R+LGVL+ L     S + F  QM +  TW 
Sbjct: 87  VRDSVGKKKQEKAALVDCVEMIGDSVDELSRTLGVLKHLRVSGGSAKEFRWQMSNAQTWA 146

Query: 146 SAALTDEDTCVEGFEG-EEGKVVTLLRNRVVKVGYITSNALALVNKL 187
           SAALTD+DTC++GF+G ++G++ T ++  + KV  +TSNAL +VN+L
Sbjct: 147 SAALTDDDTCLDGFQGMDDGEIKTEVKQWMTKVARVTSNALYMVNQL 193

[Close]

BLAST of Csa7G397050 vs. NCBI nr
Match: gi|778728057|ref|XP_011659361.1| (PREDICTED: 21 kDa protein [Cucumis sativus])

HSP 1 Score: 382.5 bits (981), Expect = 4.5e-103
Identity = 197/197 (100.00%), Postives = 197/197 (100.00%), Query Frame = 1


Query: 1   MLRAPSRRASIIALLALISILPWLTHSAKTSYVQEACRVTRHQDLCIQSLSPFSSAAKRS 60
           MLRAPSRRASIIALLALISILPWLTHSAKTSYVQEACRVTRHQDLCIQSLSPFSSAAKRS
Sbjct: 1   MLRAPSRRASIIALLALISILPWLTHSAKTSYVQEACRVTRHQDLCIQSLSPFSSAAKRS 60

Query: 61  PTKWARAGVSVTITEAKKVAGLLGRLKNNKRMKGRNRAAVLDCVEVFEAAIDELHRSLGV 120
           PTKWARAGVSVTITEAKKVAGLLGRLKNNKRMKGRNRAAVLDCVEVFEAAIDELHRSLGV
Sbjct: 61  PTKWARAGVSVTITEAKKVAGLLGRLKNNKRMKGRNRAAVLDCVEVFEAAIDELHRSLGV 120

Query: 121 LRRLSRRNFDAQMGDLTTWVSAALTDEDTCVEGFEGEEGKVVTLLRNRVVKVGYITSNAL 180
           LRRLSRRNFDAQMGDLTTWVSAALTDEDTCVEGFEGEEGKVVTLLRNRVVKVGYITSNAL
Sbjct: 121 LRRLSRRNFDAQMGDLTTWVSAALTDEDTCVEGFEGEEGKVVTLLRNRVVKVGYITSNAL 180

Query: 181 ALVNKLAASSFETTINM 198
           ALVNKLAASSFETTINM
Sbjct: 181 ALVNKLAASSFETTINM 197

[Close]

BLAST of Csa7G397050 vs. NCBI nr
Match: gi|659101359|ref|XP_008451567.1| (PREDICTED: 21 kDa protein [Cucumis melo])

HSP 1 Score: 357.5 bits (916), Expect = 1.6e-95
Identity = 182/197 (92.39%), Postives = 187/197 (94.92%), Query Frame = 1


Query: 1   MLRAPSRRASIIALLALISILPWLTHSAKTSYVQEACRVTRHQDLCIQSLSPFSSAAKRS 60
           M+R PS RASI ALLALISILPWLTHSAKTSYVQEACRVT HQDLCIQSLSPFSS AKRS
Sbjct: 1   MMRPPSTRASIAALLALISILPWLTHSAKTSYVQEACRVTLHQDLCIQSLSPFSSTAKRS 60

Query: 61  PTKWARAGVSVTITEAKKVAGLLGRLKNNKRMKGRNRAAVLDCVEVFEAAIDELHRSLGV 120
           PTKWARAGVSVTITEAKKVA LLGRLKNNKRMKGRNRAAVLDCVEVF+AAIDELHRSLGV
Sbjct: 61  PTKWARAGVSVTITEAKKVAALLGRLKNNKRMKGRNRAAVLDCVEVFDAAIDELHRSLGV 120

Query: 121 LRRLSRRNFDAQMGDLTTWVSAALTDEDTCVEGFEGEEGKVVTLLRNRVVKVGYITSNAL 180
           LRRLSRRNFD+QMGDLTTWVSAALTDEDTCVEGFEGE GKVV+LLRNRVVKVGYITSNAL
Sbjct: 121 LRRLSRRNFDSQMGDLTTWVSAALTDEDTCVEGFEGERGKVVSLLRNRVVKVGYITSNAL 180

Query: 181 ALVNKLAASSFETTINM 198
           ALVNKLA S FET +NM
Sbjct: 181 ALVNKLATSGFETALNM 197

[Close]

BLAST of Csa7G397050 vs. NCBI nr
Match: gi|388500440|gb|AFK38286.1| (unknown [Lotus japonicus])

HSP 1 Score: 217.6 bits (553), Expect = 1.9e-53
Identity = 115/190 (60.53%), Postives = 140/190 (73.68%), Query Frame = 1


Query: 12  IALLALISILPWLTHSAKT-----------SYVQEACRVTRHQDLCIQSLSPFSSAAKRS 71
           + LL +  +LP L ++ +            S V+EAC VTR+Q+LCI +LS FS+ A  S
Sbjct: 4   LMLLLITLLLPLLANTGQNQMVFAKGKNNNSNVREACSVTRYQNLCIHTLSHFSNTAGTS 63

Query: 72  PTKWARAGVSVTITEAKKVAGLLGRLKNNKRM-KGRNRAAVLDCVEVFEAAIDELHRSLG 131
           P+KWARAGVSVTITE K V   L +LK NKRM KGRNRAA+ DCVE F  A+DELHRSLG
Sbjct: 64  PSKWARAGVSVTITEVKNVQAYLTKLKKNKRMMKGRNRAALSDCVECFADALDELHRSLG 123

Query: 132 VLRRLSRRNFDAQMGDLTTWVSAALTDEDTCVEGFEGEEGKVVTLLRNRVVKVGYITSNA 190
           VLRRLS++ F  QMGDL TW+SAALTDEDTC++GFEG+  + + LL+NRV  V YITSNA
Sbjct: 124 VLRRLSKKTFSTQMGDLNTWISAALTDEDTCIDGFEGKTERQIKLLQNRVQNVSYITSNA 183

[Close]

BLAST of Csa7G397050 vs. NCBI nr
Match: gi|743870288|ref|XP_011033536.1| (PREDICTED: 21 kDa protein [Populus euphratica])

HSP 1 Score: 215.3 bits (547), Expect = 9.5e-53
Identity = 108/184 (58.70%), Postives = 137/184 (74.46%), Query Frame = 1


Query: 12  IALLALISILPWLTHSAK------TSYVQEACRVTRHQDLCIQSLSPFSSAAKRSPTKWA 71
           I++L +   L WLT +         +YV+EAC VTR+ DLC+ SL+ FS  A RSP+KWA
Sbjct: 4   ISILIIFLFLSWLTCATSWGSDNGDTYVREACSVTRYHDLCMHSLASFSHTAGRSPSKWA 63

Query: 72  RAGVSVTITEAKKVAGLLGRLKNNKRMKGRNRAAVLDCVEVFEAAIDELHRSLGVLRRLS 131
           RAGVSVTI EAK  +  L +LK +  M+GRNR A+ DC+E F+ A+D LHRSLG+LR+L 
Sbjct: 64  RAGVSVTIGEAKNASQYLNKLKKDGIMRGRNRIALSDCIECFQDAMDNLHRSLGILRKLD 123

Query: 132 RRNFDAQMGDLTTWVSAALTDEDTCVEGFEGEEGKVVTLLRNRVVKVGYITSNALALVNK 190
             NFD QMGDLTTW+SAALTDEDTC++GFE +  K V +L+N+V +V YITSNALALVNK
Sbjct: 124 ATNFDTQMGDLTTWLSAALTDEDTCLDGFEDQSSKQVNMLQNQVTRVTYITSNALALVNK 183

[Close]

BLAST of Csa7G397050 vs. NCBI nr
Match: gi|823209745|ref|XP_012438042.1| (PREDICTED: 21 kDa protein [Gossypium raimondii])

HSP 1 Score: 215.3 bits (547), Expect = 9.5e-53
Identity = 105/186 (56.45%), Postives = 139/186 (74.73%), Query Frame = 1


Query: 12  IALLALISILPWL--THSAKTSYVQEACRVTRHQDLCIQSLSPFSSAAKRSPTKWARAGV 71
           + LL L+S+L W+  T  + T+YVQ+AC VTR++ LCI +L+PFSS AK SP+KWARAGV
Sbjct: 10  LTLLLLLSVLKWVQSTPHSSTNYVQDACSVTRYRALCINTLAPFSSTAKTSPSKWARAGV 69

Query: 72  SVTITEAKKVAGLLGRLKNNKRMKGRNRAAVLDCVEVFEAAIDELHRSLGVLRRLSRRNF 131
           S+TI E KKV   L ++KN + MKG  +  + DC+E  + AID+LH SLGVLR+L+ RNF
Sbjct: 70  SITIGETKKVTQYLMKVKNYRTMKGSYKIPLSDCIECLQDAIDQLHGSLGVLRKLNARNF 129

Query: 132 DAQMGDLTTWVSAALTDEDTCVEGFEGEEGKVVTLLRNRVVKVGYITSNALALVNKLAAS 191
            AQMGD+TTW+SA LTD +TC++GFE   GK   ++RNRV++  Y TSNALALVNKLA S
Sbjct: 130 YAQMGDITTWLSAVLTDHETCLDGFENPRGKQAKMVRNRVLRSSYFTSNALALVNKLATS 189

Query: 192 SFETTI 196
             + T+
Sbjct: 190 GLDNTV 195

The following BLAST results are available for this feature:

BLAST of Csa7G397050 vs. Swiss-Prot


Match Name	E-value	Identity	Description
21KD_DAUCA	2.6e-26	35.87	21 kDa protein OS=Daucus carota PE=2 SV=1	[more]
PME3_ARATH	2.8e-12	27.85	Pectinesterase/pectinesterase inhibitor 3 OS=Arabidopsis thaliana GN=PME3 PE=2 S...	[more]
PME1_CITSI	1.1e-11	28.30	Pectinesterase 1 OS=Citrus sinensis GN=PECS-1.1 PE=2 SV=1	[more]
PME3_CITSI	1.1e-11	28.30	Pectinesterase 3 OS=Citrus sinensis PE=1 SV=1	[more]
PME34_ARATH	1.4e-11	30.68	Probable pectinesterase/pectinesterase inhibitor 34 OS=Arabidopsis thaliana GN=P...	[more]

BLAST of Csa7G397050 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KAX0_CUCSA	3.1e-103	100.00	Uncharacterized protein OS=Cucumis sativus GN=Csa_7G397050 PE=4 SV=1	[more]
I3SDE4_LOTJA	1.3e-53	60.53	Uncharacterized protein OS=Lotus japonicus PE=2 SV=1	[more]
A0A0D2T616_GOSRA	6.6e-53	56.45	Uncharacterized protein OS=Gossypium raimondii GN=B456_008G145300 PE=4 SV=1	[more]
G7LBZ5_MEDTR	3.3e-52	56.48	Plant invertase/pectin methylesterase inhibitor OS=Medicago truncatula GN=MTR_7g...	[more]
B9I9R5_POPTR	4.3e-52	58.70	Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0014s12390g PE=4 SV=1	[more]

BLAST of Csa7G397050 vs. TAIR10


Match Name	E-value	Identity	Description
AT3G62820.1	4.9e-39	51.50	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT2G47670.1	1.8e-33	45.96	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT5G62360.1	4.4e-32	39.56	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT4G12390.1	6.6e-28	37.87	pectin methylesterase inhibitor 1	[more]
AT5G20740.1	4.7e-26	35.33	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]

BLAST of Csa7G397050 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|778728057\|ref\|XP_011659361.1\|	4.5e-103	100.00	PREDICTED: 21 kDa protein [Cucumis sativus]	[more]
gi\|659101359\|ref\|XP_008451567.1\|	1.6e-95	92.39	PREDICTED: 21 kDa protein [Cucumis melo]	[more]
gi\|388500440\|gb\|AFK38286.1\|	1.9e-53	60.53	unknown [Lotus japonicus]	[more]
gi\|743870288\|ref\|XP_011033536.1\|	9.5e-53	58.70	PREDICTED: 21 kDa protein [Populus euphratica]	[more]
gi\|823209745\|ref\|XP_012438042.1\|	9.5e-53	56.45	PREDICTED: 21 kDa protein [Gossypium raimondii]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR006501	Pectinesterase_inhib_dom

Vocabulary: **Molecular Function**
Term	Definition
GO:0004857	enzyme inhibitor activity

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0043086	negative regulation of catalytic activity
biological_process	GO:0005982	starch metabolic process
biological_process	GO:0005985	sucrose metabolic process
biological_process	GO:0008150	biological_process
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0004857	enzyme inhibitor activity
molecular_function	GO:0030599	pectinesterase activity

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Csa7G397050.1	Csa7G397050.1	mRNA

InterPro

Analysis Name: InterPro Annotations of cucumber (Chinese Long)
Date Performed: 2016-09-28


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006501	Pectinesterase inhibitor domain	GENE3D	G3DSA:1.20.140.40		coord: 29..186 score: 1.0
IPR006501	Pectinesterase inhibitor domain	PFAM	PF04043	PMEI	coord: 32..182 score: 2.1
IPR006501	Pectinesterase inhibitor domain	SMART	SM00856	PMEI_2	coord: 28..182 score: 6.6
IPR006501	Pectinesterase inhibitor domain	TIGRFAMs	TIGR01614	TIGR01614	coord: 11..186 score: 3.9
IPR006501	Pectinesterase inhibitor domain	unknown	SSF101148	Plant invertase/pectin methylesterase inhibitor	coord: 31..186 score: 6.67
None	No IPR available	PANTHER	PTHR31080	FAMILY NOT NAMED	coord: 1..196 score: 1.3
None	No IPR available	PANTHER	PTHR31080:SF16	SUBFAMILY NOT NAMED	coord: 1..196 score: 1.3

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Csa7G397050	Cla015069	Watermelon (97103) v1	cuwmB568
Csa7G397050	MELO3C016001	Melon (DHL92) v3.5.1	cumeB532
Csa7G397050	ClCG09G005870	Watermelon (Charleston Gray)	cuwcgB526
Csa7G397050	Lsi02G024050	Bottle gourd (USVL1VR-Ls)	culsiB503
Csa7G397050	MELO3C016001.2	Melon (DHL92) v3.6.1	cumedB511
Csa7G397050	Cla97C09G168370	Watermelon (97103) v2	cuwmbB572
Csa7G397050	Bhi09G000717	Wax gourd	cuwgoB691
Csa7G397050	CSPI07G17480	Wild cucumber (PI 183967)	cpicuB354
Csa7G397050	Cucsa.205560	Cucumber (Gy14) v1	cgycuB312
Csa7G397050	CmaCh08G004230	Cucurbita maxima (Rimu)	cmacuB914
Csa7G397050	CmaCh17G010870	Cucurbita maxima (Rimu)	cmacuB388
Csa7G397050	CmoCh08G004170	Cucurbita moschata (Rifu)	cmocuB900
Csa7G397050	Cp4.1LG12g05670	Cucurbita pepo (Zucchini)	cpecuB161
Csa7G397050	Cp4.1LG17g06970	Cucurbita pepo (Zucchini)	cpecuB343
Csa7G397050	CsGy7G015920	Cucumber (Gy14) v2	cgybcuB323
Csa7G397050	Carg12101	Silver-seed gourd	carcuB0594
Csa7G397050	Carg07171	Silver-seed gourd	carcuB0119

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Csa7G397050	Cucurbita moschata (Rifu)	cmocuB378
Csa7G397050	Watermelon (Charleston Gray)	cuwcgB531
Csa7G397050	Watermelon (97103) v1	cuwmB588
Csa7G397050	Watermelon (97103) v1	cuwmB574
Csa7G397050	Bottle gourd (USVL1VR-Ls)	culsiB516
Csa7G397050	Watermelon (97103) v2	cuwmbB517
Csa7G397050	Watermelon (97103) v2	cuwmbB539
Csa7G397050	Wax gourd	cuwgoB650
Csa7G397050	Wax gourd	cuwgoB657
Csa7G397050	Cucumber (Chinese Long) v2	cucuB149