Carg12101 (gene) Silver-seed gourd | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Carg12101
Type	gene
Organism	Cucurbita argyrosperma (Silver-seed gourd)
Description	21 kDa protein
Location	Cucurbita_argyrosperma_scaffold_037 : 1237290 .. 1237883 (-)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSexon

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTTGCGTGCACCCACTTCAAGAACCTCAATCGTCACCCTTTTAGCCTTCATTTCAATTCTTCCATGGCTGACACACTCAGCCCAAACGAGCTACGTCCAAGAAGCGTGCAGCGTAACACTACACCCAGATCTCTGCATCCGTTCGCTGTCGCCATTTTCAAGCACCGCCAAGCGAAGCCCTACAAAGTGGGCAAGGGCGAGCGTGTCGGTGACGATAACTGAGGCCAAAGCCGTGGCGAGGTTCTTGGTGAGGCTGAAGAACAACAGACGTATGAAGGGAAGAAACAGGGCTGCAGTTTCGGACTGTGTGGAGGTCTTTGAAGACGCCATTGATGAGCTTCACAGGTCACTTGGGGTTCTTAGAAGACTGAGTAGGAGCAAATTTGACGCACAAATGGGGGATCTGACGACGTGGGTTAGCGCTGCTCTGACAGATGAAGATACTTGCGTGGATGGATTCGAAGGGGAAAGGGGAAGATTAGTGAATTTGATACGGAATCGTGTGGTGAAGGCTACTTATATTACCAGTAATGCGCTCGCTTTGGTTAACAAATTGGCTGCCTCTGGTTTTGAAACTGCCCCCAATCTATGA

mRNA sequence

ATGTTGCGTGCACCCACTTCAAGAACCTCAATCGTCACCCTTTTAGCCTTCATTTCAATTCTTCCATGGCTGACACACTCAGCCCAAACGAGCTACGTCCAAGAAGCGTGCAGCGTAACACTACACCCAGATCTCTGCATCCGTTCGCTGTCGCCATTTTCAAGCACCGCCAAGCGAAGCCCTACAAAGTGGGCAAGGGCGAGCGTGTCGGTGACGATAACTGAGGCCAAAGCCGTGGCGAGGTTCTTGGTGAGGCTGAAGAACAACAGACGTATGAAGGGAAGAAACAGGGCTGCAGTTTCGGACTGTGTGGAGGTCTTTGAAGACGCCATTGATGAGCTTCACAGGTCACTTGGGGTTCTTAGAAGACTGAGTAGGAGCAAATTTGACGCACAAATGGGGGATCTGACGACGTGGGTTAGCGCTGCTCTGACAGATGAAGATACTTGCGTGGATGGATTCGAAGGGGAAAGGGGAAGATTAGTGAATTTGATACGGAATCGTGTGGTGAAGGCTACTTATATTACCAGTAATGCGCTCGCTTTGGTTAACAAATTGGCTGCCTCTGGTTTTGAAACTGCCCCCAATCTATGA

Coding sequence (CDS)

ATGTTGCGTGCACCCACTTCAAGAACCTCAATCGTCACCCTTTTAGCCTTCATTTCAATTCTTCCATGGCTGACACACTCAGCCCAAACGAGCTACGTCCAAGAAGCGTGCAGCGTAACACTACACCCAGATCTCTGCATCCGTTCGCTGTCGCCATTTTCAAGCACCGCCAAGCGAAGCCCTACAAAGTGGGCAAGGGCGAGCGTGTCGGTGACGATAACTGAGGCCAAAGCCGTGGCGAGGTTCTTGGTGAGGCTGAAGAACAACAGACGTATGAAGGGAAGAAACAGGGCTGCAGTTTCGGACTGTGTGGAGGTCTTTGAAGACGCCATTGATGAGCTTCACAGGTCACTTGGGGTTCTTAGAAGACTGAGTAGGAGCAAATTTGACGCACAAATGGGGGATCTGACGACGTGGGTTAGCGCTGCTCTGACAGATGAAGATACTTGCGTGGATGGATTCGAAGGGGAAAGGGGAAGATTAGTGAATTTGATACGGAATCGTGTGGTGAAGGCTACTTATATTACCAGTAATGCGCTCGCTTTGGTTAACAAATTGGCTGCCTCTGGTTTTGAAACTGCCCCCAATCTATGA

Protein sequence

MLRAPTSRTSIVTLLAFISILPWLTHSAQTSYVQEACSVTLHPDLCIRSLSPFSSTAKRSPTKWARASVSVTITEAKAVARFLVRLKNNRRMKGRNRAAVSDCVEVFEDAIDELHRSLGVLRRLSRSKFDAQMGDLTTWVSAALTDEDTCVDGFEGERGRLVNLIRNRVVKATYITSNALALVNKLAASGFETAPNL

Homology

[Close]

BLAST of Carg12101 vs. NCBI nr
Match: XP_023514751.1 (pectinesterase inhibitor 6 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 379.4 bits (973), Expect = 7.4e-102
Identity = 197/197 (100.00%), Postives = 197/197 (100.00%), Query Frame = 0


Query: 1   MLRAPTSRTSIVTLLAFISILPWLTHSAQTSYVQEACSVTLHPDLCIRSLSPFSSTAKRS 60
           MLRAPTSRTSIVTLLAFISILPWLTHSAQTSYVQEACSVTLHPDLCIRSLSPFSSTAKRS
Sbjct: 1   MLRAPTSRTSIVTLLAFISILPWLTHSAQTSYVQEACSVTLHPDLCIRSLSPFSSTAKRS 60

Query: 61  PTKWARASVSVTITEAKAVARFLVRLKNNRRMKGRNRAAVSDCVEVFEDAIDELHRSLGV 120
           PTKWARASVSVTITEAKAVARFLVRLKNNRRMKGRNRAAVSDCVEVFEDAIDELHRSLGV
Sbjct: 61  PTKWARASVSVTITEAKAVARFLVRLKNNRRMKGRNRAAVSDCVEVFEDAIDELHRSLGV 120

Query: 121 LRRLSRSKFDAQMGDLTTWVSAALTDEDTCVDGFEGERGRLVNLIRNRVVKATYITSNAL 180
           LRRLSRSKFDAQMGDLTTWVSAALTDEDTCVDGFEGERGRLVNLIRNRVVKATYITSNAL
Sbjct: 121 LRRLSRSKFDAQMGDLTTWVSAALTDEDTCVDGFEGERGRLVNLIRNRVVKATYITSNAL 180

Query: 181 ALVNKLAASGFETAPNL 198
           ALVNKLAASGFETAPNL
Sbjct: 181 ALVNKLAASGFETAPNL 197

[Close]

BLAST of Carg12101 vs. NCBI nr
Match: XP_023004663.1 (pectinesterase inhibitor 6 [Cucurbita maxima])

HSP 1 Score: 378.3 bits (970), Expect = 1.6e-101
Identity = 196/197 (99.49%), Postives = 197/197 (100.00%), Query Frame = 0


Query: 1   MLRAPTSRTSIVTLLAFISILPWLTHSAQTSYVQEACSVTLHPDLCIRSLSPFSSTAKRS 60
           MLRAPTSRTSIVTLLAFI+ILPWLTHSAQTSYVQEACSVTLHPDLCIRSLSPFSSTAKRS
Sbjct: 1   MLRAPTSRTSIVTLLAFIAILPWLTHSAQTSYVQEACSVTLHPDLCIRSLSPFSSTAKRS 60

Query: 61  PTKWARASVSVTITEAKAVARFLVRLKNNRRMKGRNRAAVSDCVEVFEDAIDELHRSLGV 120
           PTKWARASVSVTITEAKAVARFLVRLKNNRRMKGRNRAAVSDCVEVFEDAIDELHRSLGV
Sbjct: 61  PTKWARASVSVTITEAKAVARFLVRLKNNRRMKGRNRAAVSDCVEVFEDAIDELHRSLGV 120

Query: 121 LRRLSRSKFDAQMGDLTTWVSAALTDEDTCVDGFEGERGRLVNLIRNRVVKATYITSNAL 180
           LRRLSRSKFDAQMGDLTTWVSAALTDEDTCVDGFEGERGRLVNLIRNRVVKATYITSNAL
Sbjct: 121 LRRLSRSKFDAQMGDLTTWVSAALTDEDTCVDGFEGERGRLVNLIRNRVVKATYITSNAL 180

Query: 181 ALVNKLAASGFETAPNL 198
           ALVNKLAASGFETAPNL
Sbjct: 181 ALVNKLAASGFETAPNL 197

[Close]

BLAST of Carg12101 vs. NCBI nr
Match: XP_022960074.1 (pectinesterase inhibitor 6-like [Cucurbita moschata])

HSP 1 Score: 376.3 bits (965), Expect = 6.3e-101
Identity = 195/197 (98.98%), Postives = 195/197 (98.98%), Query Frame = 0


Query: 1   MLRAPTSRTSIVTLLAFISILPWLTHSAQTSYVQEACSVTLHPDLCIRSLSPFSSTAKRS 60
           MLRAPTSRTSIVTLLAFISILPWLTHSAQTSYVQEACSVTLHPDLCIRSLSPFSSTAKRS
Sbjct: 63  MLRAPTSRTSIVTLLAFISILPWLTHSAQTSYVQEACSVTLHPDLCIRSLSPFSSTAKRS 122

Query: 61  PTKWARASVSVTITEAKAVARFLVRLKNNRRMKGRNRAAVSDCVEVFEDAIDELHRSLGV 120
           PTKWARASVSVTITEAKAVARFLVRLKNNRRMKGRNRAAVSDCVEVFEDAIDELHRSLGV
Sbjct: 123 PTKWARASVSVTITEAKAVARFLVRLKNNRRMKGRNRAAVSDCVEVFEDAIDELHRSLGV 182

Query: 121 LRRLSRSKFDAQMGDLTTWVSAALTDEDTCVDGFEGERGRLVNLIRNRVVKATYITSNAL 180
           LRRLSRSKFDAQMGDLTTWVSAALTDEDTCVDGFEGERGRLVNLIRNR VKATYITSNAL
Sbjct: 183 LRRLSRSKFDAQMGDLTTWVSAALTDEDTCVDGFEGERGRLVNLIRNRAVKATYITSNAL 242

Query: 181 ALVNKLAASGFETAPNL 198
           ALVNKLAASGFET PNL
Sbjct: 243 ALVNKLAASGFETGPNL 259

[Close]

BLAST of Carg12101 vs. NCBI nr
Match: XP_008451567.1 (PREDICTED: 21 kDa protein [Cucumis melo])

HSP 1 Score: 317.8 bits (813), Expect = 2.6e-83
Identity = 163/197 (82.74%), Postives = 177/197 (89.85%), Query Frame = 0


Query: 1   MLRAPTSRTSIVTLLAFISILPWLTHSAQTSYVQEACSVTLHPDLCIRSLSPFSSTAKRS 60
           M+R P++R SI  LLA ISILPWLTHSA+TSYVQEAC VTLH DLCI+SLSPFSSTAKRS
Sbjct: 1   MMRPPSTRASIAALLALISILPWLTHSAKTSYVQEACRVTLHQDLCIQSLSPFSSTAKRS 60

Query: 61  PTKWARASVSVTITEAKAVARFLVRLKNNRRMKGRNRAAVSDCVEVFEDAIDELHRSLGV 120
           PTKWARA VSVTITEAK VA  L RLKNN+RMKGRNRAAV DCVEVF+ AIDELHRSLGV
Sbjct: 61  PTKWARAGVSVTITEAKKVAALLGRLKNNKRMKGRNRAAVLDCVEVFDAAIDELHRSLGV 120

Query: 121 LRRLSRSKFDAQMGDLTTWVSAALTDEDTCVDGFEGERGRLVNLIRNRVVKATYITSNAL 180
           LRRLSR  FD+QMGDLTTWVSAALTDEDTCV+GFEGERG++V+L+RNRVVK  YITSNAL
Sbjct: 121 LRRLSRRNFDSQMGDLTTWVSAALTDEDTCVEGFEGERGKVVSLLRNRVVKVGYITSNAL 180

Query: 181 ALVNKLAASGFETAPNL 198
           ALVNKLA SGFETA N+
Sbjct: 181 ALVNKLATSGFETALNM 197

[Close]

BLAST of Carg12101 vs. NCBI nr
Match: XP_011659361.1 (PREDICTED: 21 kDa protein [Cucumis sativus] >KGN44941.1 hypothetical protein Csa_7G397050 [Cucumis sativus])

HSP 1 Score: 313.9 bits (803), Expect = 3.8e-82
Identity = 163/197 (82.74%), Postives = 173/197 (87.82%), Query Frame = 0


Query: 1   MLRAPTSRTSIVTLLAFISILPWLTHSAQTSYVQEACSVTLHPDLCIRSLSPFSSTAKRS 60
           MLRAP+ R SI+ LLA ISILPWLTHSA+TSYVQEAC VT H DLCI+SLSPFSS AKRS
Sbjct: 1   MLRAPSRRASIIALLALISILPWLTHSAKTSYVQEACRVTRHQDLCIQSLSPFSSAAKRS 60

Query: 61  PTKWARASVSVTITEAKAVARFLVRLKNNRRMKGRNRAAVSDCVEVFEDAIDELHRSLGV 120
           PTKWARA VSVTITEAK VA  L RLKNN+RMKGRNRAAV DCVEVFE AIDELHRSLGV
Sbjct: 61  PTKWARAGVSVTITEAKKVAGLLGRLKNNKRMKGRNRAAVLDCVEVFEAAIDELHRSLGV 120

Query: 121 LRRLSRSKFDAQMGDLTTWVSAALTDEDTCVDGFEGERGRLVNLIRNRVVKATYITSNAL 180
           LRRLSR  FDAQMGDLTTWVSAALTDEDTCV+GFEGE G++V L+RNRVVK  YITSNAL
Sbjct: 121 LRRLSRRNFDAQMGDLTTWVSAALTDEDTCVEGFEGEEGKVVTLLRNRVVKVGYITSNAL 180

Query: 181 ALVNKLAASGFETAPNL 198
           ALVNKLAAS FET  N+
Sbjct: 181 ALVNKLAASSFETTINM 197

[Close]

BLAST of Carg12101 vs. TAIR10
Match: AT3G62820.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein)

HSP 1 Score: 168.7 bits (426), Expect = 3.6e-42
Identity = 96/194 (49.48%), Postives = 134/194 (69.07%), Query Frame = 0


Query: 2   LRAPTSRTSIVTLLAF-ISILPWLT-HSAQTSYVQEACSVTLHPDLCIRSLSPFSSTAKR 61
           ++ P S +    L+ F +S+ P  +  S + SYVQ ACSVT + DLC ++L PF+S AK 
Sbjct: 1   MKTPMSSSITFALVFFLLSLNPTSSLPSKRESYVQNACSVTRYQDLCAKTLLPFASVAKN 60

Query: 62  SPTKWARASVSVTITEAKAVARFLVRLKNNRRMKGRNRAAVSDCVEVFEDAIDELHRSLG 121
           SP+KWARA VSV IT+ K V R L++ + +   K R+R A+SDC E+ +D++D LH+SL 
Sbjct: 61  SPSKWARAGVSVAITDNKDVLRHLLKTRLSTIGK-RDRIALSDCRELLQDSLDSLHKSLA 120

Query: 122 VLRRLSRSKFDAQMGDLTTWVSAALTDEDTCVDGFEGERGR---LVNLIRNRVVKATYIT 181
           VLR L  S+F  QM DL TW+S++LTD+DTC+DGFE    R    V +IR RV  + Y++
Sbjct: 121 VLRTLRASEFQQQMSDLATWLSSSLTDKDTCLDGFEKTSTRSSSTVRMIRKRVTTSMYLS 180

Query: 182 SNALALVNKLAASG 191
           SN+LAL+NKLAA+G
Sbjct: 181 SNSLALLNKLAANG 193

[Close]

BLAST of Carg12101 vs. TAIR10
Match: AT2G47670.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein)

HSP 1 Score: 153.3 bits (386), Expect = 1.6e-37
Identity = 82/164 (50.00%), Postives = 113/164 (68.90%), Query Frame = 0


Query: 30  TSYVQEACSVTLHPDLCIRSLSPFSSTAKRSPTKWARASVSVTITEAKAVARFLVRLKNN 89
           ++YV+ AC+VT +  LC+R+L PF+  A+ + +KWARASV+VTIT+ K V R L  LK  
Sbjct: 46  STYVRNACNVTRYNRLCVRTLWPFAIVARNNTSKWARASVAVTITDTKRVLRLL--LKTQ 105

Query: 90  RRMKGRN-RAAVSDCVEVFEDAIDELHRSLGVLRRLSRSKFDAQMGDLTTWVSAALTDED 149
           R   G + R A+SDC E+F D++D L++SL VLR L+  +F  Q+ DL TW+SAALTD+D
Sbjct: 106 RSAVGESERIALSDCRELFVDSLDNLYKSLAVLRTLNADEFQRQISDLATWLSAALTDDD 165

Query: 150 TCVDGFE--GERGRLVNLIRNRVVKATYITSNALALVNKLAASG 191
           TC+DGFE    R R V ++R +  K   + SNALAL+ KLA  G
Sbjct: 166 TCLDGFEETSSRTRTVRMVRRKATKCMRLCSNALALLKKLAFDG 207

[Close]

BLAST of Carg12101 vs. TAIR10
Match: AT1G62760.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein)

HSP 1 Score: 118.2 bits (295), Expect = 5.6e-27
Identity = 59/161 (36.65%), Postives = 102/161 (63.35%), Query Frame = 0


Query: 32  YVQEACSVTLHPDLCIRSLSPFSSTAKRSPTKWARASVSVTITEAKAVARFLVRLKNNRR 91
           Y++ +C++TL+  +C  SLSP++ST + +P K A  ++++T++ AK+ ++F+  + +   
Sbjct: 147 YIKTSCNITLYKTICYNSLSPYASTIRSNPQKLAVIALNLTLSSAKSASKFVKNISHGGG 206

Query: 92  MKGRNRAAVSDCVEVFEDAIDELH---RSLGVLRRLSRSKFDAQMGDLTTWVSAALTDED 151
           +      AV+DCVE   D++  L    R L  +     +KF+  M D+ TWVSAALT++D
Sbjct: 207 LTRLEVVAVADCVEEIGDSVTSLQDSIRELDSINYKDSAKFEMVMSDVETWVSAALTNDD 266

Query: 152 TCVDGFEGERGRLVNLIRNRVVKATYITSNALALVNKLAAS 190
           TC+DGF   +  + +L+R  VV+   +TSNALAL+N  A++
Sbjct: 267 TCMDGFSLVKTAVKDLVRRHVVEVARLTSNALALINMYAST 307

[Close]

BLAST of Carg12101 vs. TAIR10
Match: AT2G01610.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein)

HSP 1 Score: 117.1 bits (292), Expect = 1.2e-26
Identity = 70/187 (37.43%), Postives = 106/187 (56.68%), Query Frame = 0


Query: 22  PWLTHSAQTSYVQEACSVTLHPDLCIRSLSPFSSTAKRSPTKWARASVSVTITEAKAVAR 81
           P  T +    +++ +C+ TL+PD+C  SLS ++S  + SP + A+ ++ V++++AK+ A 
Sbjct: 34  PNTTTTNDLDFIRTSCNATLYPDVCFTSLSGYASAVQDSPARLAKLAIGVSLSQAKSTAA 93

Query: 82  FLVRLKNNRRMKGRN-----RAAVSDCVEVFEDAIDELHRSLGVLR-----------RLS 141
           FL +L  +      +      A + DCV   EDA+DE+  SL  LR           R S
Sbjct: 94  FLSKLSRSAAKYSGDGHQTASAVIRDCVSNVEDAVDEMRGSLRQLRDMNGRGGGTAARRS 153

Query: 142 RSKFDAQMGDLTTWVSAALTDEDTCVDGFE--GERGRLVNLIRNRVVKATYITSNALALV 191
              F  QM ++ TW+SAALTDEDTC DGFE   E G +   + +R+ +   +TSNALALV
Sbjct: 154 VETFRFQMSNVQTWMSAALTDEDTCTDGFEDMDEGGLIKTTVCDRLEEVKRLTSNALALV 213

[Close]

BLAST of Carg12101 vs. TAIR10
Match: AT5G62350.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein)

HSP 1 Score: 117.1 bits (292), Expect = 1.2e-26
Identity = 61/163 (37.42%), Postives = 97/163 (59.51%), Query Frame = 0


Query: 31  SYVQEACSVTLHPDLCIRSLSPFSSTAKRSPTKWARASVSVTITEAKAVARFLVRLKNNR 90
           +++Q +C  T +P LC+ SLS +++  + SP + A  +++VT++ A++   F+ RL   +
Sbjct: 37  NFIQSSCKTTTYPALCVHSLSVYANDIQTSPKRLAETAIAVTLSRAQSTKLFVSRLTRMK 96

Query: 91  RMKGRNRAAVSDCVEVFEDAIDELHRSLGVLRRLSRSK----FDAQMGDLTTWVSAALTD 150
            +K R   A+ DCVE   D +D L +S+  L+    +K    F   M +  TW SAALTD
Sbjct: 97  GLKKREVEAIKDCVEEMNDTVDRLTKSVQELKLCGSAKDQDQFAYHMSNAQTWTSAALTD 156

Query: 151 EDTCVDGFEGE--RGRLVNLIRNRVVKATYITSNALALVNKLA 188
           E+TC DGF G    GR+ N +R R++   + TSNAL+L+N  A
Sbjct: 157 ENTCSDGFSGRVMDGRIKNSVRARIMNVGHETSNALSLINAFA 199

[Close]

BLAST of Carg12101 vs. Swiss-Prot
Match: sp|O22244|PMEI6_ARATH (Pectinesterase inhibitor 6 OS=Arabidopsis thaliana OX=3702 GN=PMEI6 PE=2 SV=1)

HSP 1 Score: 153.3 bits (386), Expect = 2.8e-36
Identity = 82/164 (50.00%), Postives = 113/164 (68.90%), Query Frame = 0


Query: 30  TSYVQEACSVTLHPDLCIRSLSPFSSTAKRSPTKWARASVSVTITEAKAVARFLVRLKNN 89
           ++YV+ AC+VT +  LC+R+L PF+  A+ + +KWARASV+VTIT+ K V R L  LK  
Sbjct: 46  STYVRNACNVTRYNRLCVRTLWPFAIVARNNTSKWARASVAVTITDTKRVLRLL--LKTQ 105

Query: 90  RRMKGRN-RAAVSDCVEVFEDAIDELHRSLGVLRRLSRSKFDAQMGDLTTWVSAALTDED 149
           R   G + R A+SDC E+F D++D L++SL VLR L+  +F  Q+ DL TW+SAALTD+D
Sbjct: 106 RSAVGESERIALSDCRELFVDSLDNLYKSLAVLRTLNADEFQRQISDLATWLSAALTDDD 165

Query: 150 TCVDGFE--GERGRLVNLIRNRVVKATYITSNALALVNKLAASG 191
           TC+DGFE    R R V ++R +  K   + SNALAL+ KLA  G
Sbjct: 166 TCLDGFEETSSRTRTVRMVRRKATKCMRLCSNALALLKKLAFDG 207

[Close]

BLAST of Carg12101 vs. Swiss-Prot
Match: sp|P17407|21KD_DAUCA (21 kDa protein OS=Daucus carota OX=4039 PE=2 SV=1)

HSP 1 Score: 122.9 bits (307), Expect = 4.1e-27
Identity = 67/184 (36.41%), Postives = 111/184 (60.33%), Query Frame = 0


Query: 10  SIVTLLAFISILPWLTHSAQTSYVQEACSVTLHPDLCIRSLSPFSSTAKRSPTKWARASV 69
           S +   A + IL   + +    +++ +C++T +P +C +SLS ++ T + +P + A  ++
Sbjct: 6   STLVFSALLVILAAASAAPANQFIKTSCTLTTYPAVCEQSLSAYAKTIQNNPQELASTAL 65

Query: 70  SVTITEAKAVARFLVRLKNNRRMKGRNRAAVSDCVEVFEDAIDELHRSLGVLRRLSRSK- 129
            V++T  +    F+ RL   + +K R  AA+ DC+E  ED++D + RS   ++ LS +K 
Sbjct: 66  QVSLTRTQQAQTFMKRLNKFKGLKARQYAAIHDCLEEVEDSLDRVSRSCDEMKNLSHAKG 125

Query: 130 --FDAQMGDLTTWVSAALTDEDTCVDGFEGE--RGRLVNLIRNRVVKATYITSNALALVN 189
             F  +M ++ TWVSAALTDE TC+DGF G+   G++   +R +VV    +TSNALALVN
Sbjct: 126 NDFTFRMSNVETWVSAALTDETTCMDGFAGKGMDGKIKESVRAQVVAVARVTSNALALVN 185

[Close]

BLAST of Carg12101 vs. Swiss-Prot
Match: sp|Q9SI74|PMI10_ARATH (Pectinesterase inhibitor 10 OS=Arabidopsis thaliana OX=3702 GN=PMEI10 PE=2 SV=1)

HSP 1 Score: 118.2 bits (295), Expect = 1.0e-25
Identity = 59/161 (36.65%), Postives = 102/161 (63.35%), Query Frame = 0


Query: 32  YVQEACSVTLHPDLCIRSLSPFSSTAKRSPTKWARASVSVTITEAKAVARFLVRLKNNRR 91
           Y++ +C++TL+  +C  SLSP++ST + +P K A  ++++T++ AK+ ++F+  + +   
Sbjct: 147 YIKTSCNITLYKTICYNSLSPYASTIRSNPQKLAVIALNLTLSSAKSASKFVKNISHGGG 206

Query: 92  MKGRNRAAVSDCVEVFEDAIDELH---RSLGVLRRLSRSKFDAQMGDLTTWVSAALTDED 151
           +      AV+DCVE   D++  L    R L  +     +KF+  M D+ TWVSAALT++D
Sbjct: 207 LTRLEVVAVADCVEEIGDSVTSLQDSIRELDSINYKDSAKFEMVMSDVETWVSAALTNDD 266

Query: 152 TCVDGFEGERGRLVNLIRNRVVKATYITSNALALVNKLAAS 190
           TC+DGF   +  + +L+R  VV+   +TSNALAL+N  A++
Sbjct: 267 TCMDGFSLVKTAVKDLVRRHVVEVARLTSNALALINMYAST 307

[Close]

BLAST of Carg12101 vs. Swiss-Prot
Match: sp|Q84WE4|PMEI3_ARATH (Pectinesterase inhibitor 3 OS=Arabidopsis thaliana OX=3702 GN=PMEI3 PE=2 SV=1)

HSP 1 Score: 116.7 bits (291), Expect = 2.9e-25
Identity = 69/193 (35.75%), Postives = 113/193 (58.55%), Query Frame = 0


Query: 4   APTSRTSIVTLLAFISILPWLT----HSAQTSYVQEACSVTLHPDLCIRSLSPFSSTAKR 63
           APT    +V  +AF  I    T    H+     V  +C    +P LC+R+LS +S     
Sbjct: 2   APTQNLFLVA-IAFAVIFTASTVHGRHNGAEDIVHSSCEHASYPSLCVRTLSSYSGPTIT 61

Query: 64  SPTKWARASVSVTITEAKAVARFLVRLKNNRRMKGRNRAAVSDCVEVFEDAIDELHRSLG 123
           +    A+A++ ++++ A++ A+ L  ++++   K + +AA+ DCVE+  D++DEL R+LG
Sbjct: 62  NRRDLAQAAIKISLSHAQSAAKKLAVVRDSVGKKKQEKAALVDCVEMIGDSVDELSRTLG 121

Query: 124 VLRRL-----SRSKFDAQMGDLTTWVSAALTDEDTCVDGFEG-ERGRLVNLIRNRVVKAT 183
           VL+ L     S  +F  QM +  TW SAALTD+DTC+DGF+G + G +   ++  + K  
Sbjct: 122 VLKHLRVSGGSAKEFRWQMSNAQTWASAALTDDDTCLDGFQGMDDGEIKTEVKQWMTKVA 181

Query: 184 YITSNALALVNKL 187
            +TSNAL +VN+L
Sbjct: 182 RVTSNALYMVNQL 193

[Close]

BLAST of Carg12101 vs. Swiss-Prot
Match: sp|Q9SB37|PMEI7_ARATH (Pectinesterase inhibitor 7 OS=Arabidopsis thaliana OX=3702 GN=PMEI7 PE=2 SV=1)

HSP 1 Score: 113.6 bits (283), Expect = 2.5e-24
Identity = 63/168 (37.50%), Postives = 96/168 (57.14%), Query Frame = 0


Query: 27  SAQTSYVQEACSVTLHPDLCIRSLSPFSSTAKRSPTKWARASVSVTITEAKAVARFLVRL 86
           S  T +++ +C  T +PD C +SLS ++S  K+ P K A  +++V+I  AK+   ++  +
Sbjct: 32  SGDTEFIKASCETTSYPDRCFQSLSSYASEIKKQPRKLAETALAVSIARAKSAKTYVSEM 91

Query: 87  KNNRRMKGRNRAAVSDCVEVFEDAIDELHRSLGVLRRL----SRSKFDAQMGDLTTWVSA 146
            + + +  R   AV+DC+E   D +D L  SL  L+ L    S   F   + ++ TW SA
Sbjct: 92  TDYKGITKRQHEAVADCLEEMGDTVDRLSNSLKELKHLEEGDSGEDFWFCLSNVRTWTSA 151

Query: 147 ALTDEDTCVDGFEGE--RGRLVNLIRNRVVKATYITSNALALVNKLAA 189
           ALTDE  C+DGF G+   G L +LIR  +V     TSNALAL+N  A+
Sbjct: 152 ALTDETACMDGFGGKAMAGELKSLIRTHIVSVAEETSNALALINDFAS 199

[Close]

BLAST of Carg12101 vs. TrEMBL
Match: tr|A0A1S3BSW5|A0A1S3BSW5_CUCME (21 kDa protein OS=Cucumis melo OX=3656 GN=LOC103492810 PE=4 SV=1)

HSP 1 Score: 317.8 bits (813), Expect = 1.7e-83
Identity = 163/197 (82.74%), Postives = 177/197 (89.85%), Query Frame = 0


Query: 1   MLRAPTSRTSIVTLLAFISILPWLTHSAQTSYVQEACSVTLHPDLCIRSLSPFSSTAKRS 60
           M+R P++R SI  LLA ISILPWLTHSA+TSYVQEAC VTLH DLCI+SLSPFSSTAKRS
Sbjct: 1   MMRPPSTRASIAALLALISILPWLTHSAKTSYVQEACRVTLHQDLCIQSLSPFSSTAKRS 60

Query: 61  PTKWARASVSVTITEAKAVARFLVRLKNNRRMKGRNRAAVSDCVEVFEDAIDELHRSLGV 120
           PTKWARA VSVTITEAK VA  L RLKNN+RMKGRNRAAV DCVEVF+ AIDELHRSLGV
Sbjct: 61  PTKWARAGVSVTITEAKKVAALLGRLKNNKRMKGRNRAAVLDCVEVFDAAIDELHRSLGV 120

Query: 121 LRRLSRSKFDAQMGDLTTWVSAALTDEDTCVDGFEGERGRLVNLIRNRVVKATYITSNAL 180
           LRRLSR  FD+QMGDLTTWVSAALTDEDTCV+GFEGERG++V+L+RNRVVK  YITSNAL
Sbjct: 121 LRRLSRRNFDSQMGDLTTWVSAALTDEDTCVEGFEGERGKVVSLLRNRVVKVGYITSNAL 180

Query: 181 ALVNKLAASGFETAPNL 198
           ALVNKLA SGFETA N+
Sbjct: 181 ALVNKLATSGFETALNM 197

[Close]

BLAST of Carg12101 vs. TrEMBL
Match: tr|A0A0A0KAX0|A0A0A0KAX0_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G397050 PE=4 SV=1)

HSP 1 Score: 313.9 bits (803), Expect = 2.5e-82
Identity = 163/197 (82.74%), Postives = 173/197 (87.82%), Query Frame = 0


Query: 1   MLRAPTSRTSIVTLLAFISILPWLTHSAQTSYVQEACSVTLHPDLCIRSLSPFSSTAKRS 60
           MLRAP+ R SI+ LLA ISILPWLTHSA+TSYVQEAC VT H DLCI+SLSPFSS AKRS
Sbjct: 1   MLRAPSRRASIIALLALISILPWLTHSAKTSYVQEACRVTRHQDLCIQSLSPFSSAAKRS 60

Query: 61  PTKWARASVSVTITEAKAVARFLVRLKNNRRMKGRNRAAVSDCVEVFEDAIDELHRSLGV 120
           PTKWARA VSVTITEAK VA  L RLKNN+RMKGRNRAAV DCVEVFE AIDELHRSLGV
Sbjct: 61  PTKWARAGVSVTITEAKKVAGLLGRLKNNKRMKGRNRAAVLDCVEVFEAAIDELHRSLGV 120

Query: 121 LRRLSRSKFDAQMGDLTTWVSAALTDEDTCVDGFEGERGRLVNLIRNRVVKATYITSNAL 180
           LRRLSR  FDAQMGDLTTWVSAALTDEDTCV+GFEGE G++V L+RNRVVK  YITSNAL
Sbjct: 121 LRRLSRRNFDAQMGDLTTWVSAALTDEDTCVEGFEGEEGKVVTLLRNRVVKVGYITSNAL 180

Query: 181 ALVNKLAASGFETAPNL 198
           ALVNKLAAS FET  N+
Sbjct: 181 ALVNKLAASSFETTINM 197

[Close]

BLAST of Carg12101 vs. TrEMBL
Match: tr|A0A2N9FQQ0|A0A2N9FQQ0_FAGSY (Uncharacterized protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS17141 PE=4 SV=1)

HSP 1 Score: 228.0 bits (580), Expect = 1.8e-56
Identity = 113/176 (64.20%), Postives = 143/176 (81.25%), Query Frame = 0


Query: 15  LAFISILPWLTHSAQTSYVQEACSVTLHPDLCIRSLSPFSSTAKRSPTKWARASVSVTIT 74
           +A+++ L    H  + +YVQEACSVT +P +C+ SL+ FSS AKRSP+KWARA VSVTI 
Sbjct: 13  VAWLTSLGQFAHGKRVTYVQEACSVTRYPAICVHSLASFSSVAKRSPSKWARAGVSVTIG 72

Query: 75  EAKAVARFLVRLKNNRRMKGRNRAAVSDCVEVFEDAIDELHRSLGVLRRLSRSKFDAQMG 134
           E K VA+ L +LK NR M+GRNR A+ DC+E F+DAIDELH+SLGVLRRLSRS FD QM 
Sbjct: 73  EVKCVAQDLNKLKKNRVMRGRNRIALLDCIECFQDAIDELHKSLGVLRRLSRSTFDDQMS 132

Query: 135 DLTTWVSAALTDEDTCVDGFEGERGRLVNLIRNRVVKATYITSNALALVNKLAASG 191
           DL TW+SAALT+EDTC+DGFE ++G  V L++N+V+ A+YITSNALALVNKLA++G
Sbjct: 133 DLNTWISAALTNEDTCLDGFEDQKGSQVKLLQNQVLNASYITSNALALVNKLASTG 188

[Close]

BLAST of Carg12101 vs. TrEMBL
Match: tr|A0A2P4HPV5|A0A2P4HPV5_QUESU (21 kDa protein OS=Quercus suber OX=58331 GN=CFP56_05286 PE=4 SV=1)

HSP 1 Score: 222.6 bits (566), Expect = 7.6e-55
Identity = 109/167 (65.27%), Postives = 138/167 (82.63%), Query Frame = 0


Query: 24  LTHSAQTSYVQEACSVTLHPDLCIRSLSPFSSTAKRSPTKWARASVSVTITEAKAVARFL 83
           L +   +SYVQEAC VT +PD+C+ SL+ FS+TAKRSP+KWARA VSVTI E K VA+ L
Sbjct: 15  LAYGNGSSYVQEACKVTRYPDMCVHSLASFSNTAKRSPSKWARAGVSVTIGEVKDVAQNL 74

Query: 84  VRLKNNRRMKGRNRAAVSDCVEVFEDAIDELHRSLGVLRRLSRSKFDAQMGDLTTWVSAA 143
            +LK  R MKG+NR A+SDC+E  +DA+DELH+SL VLRRLS+S F+ QM DL TW+SAA
Sbjct: 75  KKLKKYRLMKGKNRIALSDCIECIQDALDELHKSLDVLRRLSKSTFNEQMSDLLTWISAA 134

Query: 144 LTDEDTCVDGFEGERGRLVNLIRNRVVKATYITSNALALVNKLAASG 191
           LTDEDTC+DGFE ++G+ V L++N+V+ ATYITSNALALVNKLA++G
Sbjct: 135 LTDEDTCLDGFEDQKGKQVKLLQNQVLNATYITSNALALVNKLASTG 181

[Close]

BLAST of Carg12101 vs. TrEMBL
Match: tr|A0A1R3IHA2|A0A1R3IHA2_9ROSI (Pectinesterase inhibitor OS=Corchorus olitorius OX=93759 GN=COLO4_23347 PE=4 SV=1)

HSP 1 Score: 222.6 bits (566), Expect = 7.6e-55
Identity = 113/178 (63.48%), Postives = 142/178 (79.78%), Query Frame = 0


Query: 15  LAFISILPWLTHSAQTSYVQEACSVTLHPDLCIRSLSPFSSTAKRSPTKWARASVSVTIT 74
           L  + IL W   SA ++YVQ+ACSVT +  LCI SL+ FSSTAK SP+KWARA VSVT+ 
Sbjct: 11  LLLLWILKW-GQSAASNYVQDACSVTKYRTLCITSLASFSSTAKESPSKWARAGVSVTLG 70

Query: 75  EAKAVARFLVRLKNNRRMKGRNRAAVSDCVEVFEDAIDELHRSLGVLRRLSRSKFDAQMG 134
           E K V+++L ++KN R M+GR +  +SDCVEVFEDAID+LH SLGVLR+LS S+F AQMG
Sbjct: 71  ETKNVSKYLNKVKNYREMRGRYKIPLSDCVEVFEDAIDQLHLSLGVLRKLSASQFYAQMG 130

Query: 135 DLTTWVSAALTDEDTCVDGFEGERGRLVNLIRNRVVKATYITSNALALVNKLAASGFE 193
           D+TTW+SA LTD+DTC+DGFE  RG+   ++RNRV++A+Y TSNALALVNKLA SG E
Sbjct: 131 DVTTWLSATLTDQDTCLDGFENPRGKQAKMLRNRVLRASYFTSNALALVNKLATSGLE 187

The following BLAST results are available for this feature:

BLAST of Carg12101 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023514751.1	7.4e-102	100.00	pectinesterase inhibitor 6 [Cucurbita pepo subsp. pepo]	[more]
XP_023004663.1	1.6e-101	99.49	pectinesterase inhibitor 6 [Cucurbita maxima]	[more]
XP_022960074.1	6.3e-101	98.98	pectinesterase inhibitor 6-like [Cucurbita moschata]	[more]
XP_008451567.1	2.6e-83	82.74	PREDICTED: 21 kDa protein [Cucumis melo]	[more]
XP_011659361.1	3.8e-82	82.74	PREDICTED: 21 kDa protein [Cucumis sativus] >KGN44941.1 hypothetical protein Csa...	[more]

BLAST of Carg12101 vs. TAIR10


Match Name	E-value	Identity	Description
AT3G62820.1	3.6e-42	49.48	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT2G47670.1	1.6e-37	50.00	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT1G62760.1	5.6e-27	36.65	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT2G01610.1	1.2e-26	37.43	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT5G62350.1	1.2e-26	37.42	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]

BLAST of Carg12101 vs. Swiss-Prot


Match Name	E-value	Identity	Description
sp\|O22244\|PMEI6_ARATH	2.8e-36	50.00	Pectinesterase inhibitor 6 OS=Arabidopsis thaliana OX=3702 GN=PMEI6 PE=2 SV=1	[more]
sp\|P17407\|21KD_DAUCA	4.1e-27	36.41	21 kDa protein OS=Daucus carota OX=4039 PE=2 SV=1	[more]
sp\|Q9SI74\|PMI10_ARATH	1.0e-25	36.65	Pectinesterase inhibitor 10 OS=Arabidopsis thaliana OX=3702 GN=PMEI10 PE=2 SV=1	[more]
sp\|Q84WE4\|PMEI3_ARATH	2.9e-25	35.75	Pectinesterase inhibitor 3 OS=Arabidopsis thaliana OX=3702 GN=PMEI3 PE=2 SV=1	[more]
sp\|Q9SB37\|PMEI7_ARATH	2.5e-24	37.50	Pectinesterase inhibitor 7 OS=Arabidopsis thaliana OX=3702 GN=PMEI7 PE=2 SV=1	[more]

BLAST of Carg12101 vs. TrEMBL


Match Name	E-value	Identity	Description
tr\|A0A1S3BSW5\|A0A1S3BSW5_CUCME	1.7e-83	82.74	21 kDa protein OS=Cucumis melo OX=3656 GN=LOC103492810 PE=4 SV=1	[more]
tr\|A0A0A0KAX0\|A0A0A0KAX0_CUCSA	2.5e-82	82.74	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G397050 PE=4 SV=1	[more]
tr\|A0A2N9FQQ0\|A0A2N9FQQ0_FAGSY	1.8e-56	64.20	Uncharacterized protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS17141 PE=4 SV=1	[more]
tr\|A0A2P4HPV5\|A0A2P4HPV5_QUESU	7.6e-55	65.27	21 kDa protein OS=Quercus suber OX=58331 GN=CFP56_05286 PE=4 SV=1	[more]
tr\|A0A1R3IHA2\|A0A1R3IHA2_9ROSI	7.6e-55	63.48	Pectinesterase inhibitor OS=Corchorus olitorius OX=93759 GN=COLO4_23347 PE=4 SV=...	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **Molecular Function**
Term	Definition
GO:0004857	enzyme inhibitor activity

Vocabulary: **INTERPRO**
Term	Definition
IPR035513	Invertase/methylesterase_inhib
IPR006501	Pectinesterase_inhib_dom

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0008150	biological_process
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0004857	enzyme inhibitor activity

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg12101-RA	Carg12101-RA	mRNA

InterPro

Analysis Name: InterPro Annotations of silver-seed gourd
Date Performed: 2019-03-07


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006501	Pectinesterase inhibitor domain	SMART	SM00856	PMEI_2	coord: 28..182 e-value: 8.4E-50 score: 181.4
IPR006501	Pectinesterase inhibitor domain	TIGRFAM	TIGR01614	TIGR01614	coord: 10..186 e-value: 4.1E-37 score: 125.2
IPR006501	Pectinesterase inhibitor domain	PFAM	PF04043	PMEI	coord: 32..182 e-value: 2.2E-34 score: 118.9
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	GENE3D	G3DSA:1.20.140.40		coord: 29..189 e-value: 3.5E-26 score: 93.8
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	SUPERFAMILY	SSF101148	Plant invertase/pectin methylesterase inhibitor	coord: 31..186
None	No IPR available	PANTHER	PTHR31080:SF16	PECTIN METHYLESTERASE INHIBITOR 6-RELATED	coord: 18..192
None	No IPR available	PANTHER	PTHR31080	FAMILY NOT NAMED	coord: 18..192
None	No IPR available	CDD	cd15798	PMEI-like_3	coord: 36..188 e-value: 6.88331E-48 score: 154.904

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Carg12101	Cla015069	Watermelon (97103) v1	carwmB0559
Carg12101	Csa7G397050	Cucumber (Chinese Long) v2	carcuB0594
Carg12101	MELO3C016001	Melon (DHL92) v3.5.1	carmeB0566
Carg12101	ClCG09G005870	Watermelon (Charleston Gray)	carwcgB0547
Carg12101	CSPI07G17480	Wild cucumber (PI 183967)	carcpiB0599
Carg12101	Cucsa.205560	Cucumber (Gy14) v1	carcgyB0640
Carg12101	CmaCh08G004230	Cucurbita maxima (Rimu)	carcmaB0792
Carg12101	CmaCh17G010870	Cucurbita maxima (Rimu)	carcmaB0787
Carg12101	CmaCh16G010510	Cucurbita maxima (Rimu)	carcmaB0786
Carg12101	CmoCh16G010990	Cucurbita moschata (Rifu)	carcmoB0766
Carg12101	CmoCh08G004170	Cucurbita moschata (Rifu)	carcmoB0771
Carg12101	Lsi02G024050	Bottle gourd (USVL1VR-Ls)	carlsiB511
Carg12101	Cp4.1LG12g05670	Cucurbita pepo (Zucchini)	carcpeB0801
Carg12101	Cp4.1LG17g06970	Cucurbita pepo (Zucchini)	carcpeB0803
Carg12101	CsGy7G015920	Cucumber (Gy14) v2	carcgybB0569
Carg12101	MELO3C016001.2	Melon (DHL92) v3.6.1	carmedB0572
Carg12101	CsaV3_7G029270	Cucumber (Chinese Long) v3	carcucB0601
Carg12101	Cla97C09G168370	Watermelon (97103) v2	carwmbB0590
Carg12101	Bhi09G000717	Wax gourd	carwgoB0673

The following gene(s) are paralogous to this gene:


Gene	Paralogue	Organism	Block
Carg12101	Carg07171	Silver-seed gourd	carcarB130

The following block(s) are covering this gene:


Gene	Organism	Block
Carg12101	Cucurbita moschata (Rifu)	carcmoB0767