Cp4.1LG17g06970 (gene) Cucurbita pepo (Zucchini)

Overview
NameCp4.1LG17g06970
Typegene
OrganismCucurbita pepo (Cucurbita pepo (Zucchini))
DescriptionPlant invertase/pectin methylesterase inhibitor superfamily protein, putative
LocationCp4.1LG17 : 6182475 .. 6183068 (-)
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTTGCGTGCACCCACTTCAAGAACCTCAATCGTCACCCTTTTAGCCTTCATTTCAATTCTTCCATGGCTGACACACTCAGCCCAAACGAGCTACGTCCAAGAAGCGTGCAGCGTAACACTACACCCAGATCTCTGCATCCGTTCGCTGTCGCCATTTTCAAGCACCGCCAAGCGAAGCCCTACAAAGTGGGCAAGGGCGAGCGTGTCGGTGACGATAACTGAGGCCAAAGCCGTGGCGAGATTCTTGGTGAGGCTGAAGAACAACAGACGTATGAAGGGAAGAAACAGGGCTGCAGTTTCGGACTGTGTGGAGGTCTTTGAAGACGCCATTGATGAGCTTCACAGGTCACTTGGGGTTCTTAGAAGACTGAGTAGGAGCAAGTTCGACGCACAAATGGGGGATCTGACGACGTGGGTTAGCGCTGCTCTGACAGATGAAGACACTTGCGTGGATGGATTCGAAGGGGAAAGGGGAAGATTAGTGAATTTGATACGGAATCGTGTGGTGAAGGCTACTTATATTACCAGTAATGCGCTCGCTTTGGTTAACAAATTGGCTGCCTCAGGTTTTGAAACTGCCCCCAATCTATGA

mRNA sequence

ATGTTGCGTGCACCCACTTCAAGAACCTCAATCGTCACCCTTTTAGCCTTCATTTCAATTCTTCCATGGCTGACACACTCAGCCCAAACGAGCTACGTCCAAGAAGCGTGCAGCGTAACACTACACCCAGATCTCTGCATCCGTTCGCTGTCGCCATTTTCAAGCACCGCCAAGCGAAGCCCTACAAAGTGGGCAAGGGCGAGCGTGTCGGTGACGATAACTGAGGCCAAAGCCGTGGCGAGATTCTTGGTGAGGCTGAAGAACAACAGACGTATGAAGGGAAGAAACAGGGCTGCAGTTTCGGACTGTGTGGAGGTCTTTGAAGACGCCATTGATGAGCTTCACAGGTCACTTGGGGTTCTTAGAAGACTGAGTAGGAGCAAGTTCGACGCACAAATGGGGGATCTGACGACGTGGGTTAGCGCTGCTCTGACAGATGAAGACACTTGCGTGGATGGATTCGAAGGGGAAAGGGGAAGATTAGTGAATTTGATACGGAATCGTGTGGTGAAGGCTACTTATATTACCAGTAATGCGCTCGCTTTGGTTAACAAATTGGCTGCCTCAGGTTTTGAAACTGCCCCCAATCTATGA

Coding sequence (CDS)

ATGTTGCGTGCACCCACTTCAAGAACCTCAATCGTCACCCTTTTAGCCTTCATTTCAATTCTTCCATGGCTGACACACTCAGCCCAAACGAGCTACGTCCAAGAAGCGTGCAGCGTAACACTACACCCAGATCTCTGCATCCGTTCGCTGTCGCCATTTTCAAGCACCGCCAAGCGAAGCCCTACAAAGTGGGCAAGGGCGAGCGTGTCGGTGACGATAACTGAGGCCAAAGCCGTGGCGAGATTCTTGGTGAGGCTGAAGAACAACAGACGTATGAAGGGAAGAAACAGGGCTGCAGTTTCGGACTGTGTGGAGGTCTTTGAAGACGCCATTGATGAGCTTCACAGGTCACTTGGGGTTCTTAGAAGACTGAGTAGGAGCAAGTTCGACGCACAAATGGGGGATCTGACGACGTGGGTTAGCGCTGCTCTGACAGATGAAGACACTTGCGTGGATGGATTCGAAGGGGAAAGGGGAAGATTAGTGAATTTGATACGGAATCGTGTGGTGAAGGCTACTTATATTACCAGTAATGCGCTCGCTTTGGTTAACAAATTGGCTGCCTCAGGTTTTGAAACTGCCCCCAATCTATGA

Protein sequence

MLRAPTSRTSIVTLLAFISILPWLTHSAQTSYVQEACSVTLHPDLCIRSLSPFSSTAKRSPTKWARASVSVTITEAKAVARFLVRLKNNRRMKGRNRAAVSDCVEVFEDAIDELHRSLGVLRRLSRSKFDAQMGDLTTWVSAALTDEDTCVDGFEGERGRLVNLIRNRVVKATYITSNALALVNKLAASGFETAPNL
Homology
[Close]
BLAST of Cp4.1LG17g06970 vs. Swiss-Prot
Match: 21KD_DAUCA (21 kDa protein OS=Daucus carota PE=2 SV=1)

HSP 1 Score: 122.9 bits (307), Expect = 4.0e-27
Identity = 67/184 (36.41%), Postives = 111/184 (60.33%), Query Frame = 1 

Query: 10  SIVTLLAFISILPWLTHSAQTSYVQEACSVTLHPDLCIRSLSPFSSTAKRSPTKWARASV 69
           S +   A + IL   + +    +++ +C++T +P +C +SLS ++ T + +P + A  ++
Sbjct: 6   STLVFSALLVILAAASAAPANQFIKTSCTLTTYPAVCEQSLSAYAKTIQNNPQELASTAL 65

Query: 70  SVTITEAKAVARFLVRLKNNRRMKGRNRAAVSDCVEVFEDAIDELHRSLGVLRRLSRSK- 129
            V++T  +    F+ RL   + +K R  AA+ DC+E  ED++D + RS   ++ LS +K 
Sbjct: 66  QVSLTRTQQAQTFMKRLNKFKGLKARQYAAIHDCLEEVEDSLDRVSRSCDEMKNLSHAKG 125

Query: 130 --FDAQMGDLTTWVSAALTDEDTCVDGFEGE--RGRLVNLIRNRVVKATYITSNALALVN 189
             F  +M ++ TWVSAALTDE TC+DGF G+   G++   +R +VV    +TSNALALVN
Sbjct: 126 NDFTFRMSNVETWVSAALTDETTCMDGFAGKGMDGKIKESVRAQVVAVARVTSNALALVN 185

[Close]
BLAST of Cp4.1LG17g06970 vs. Swiss-Prot
Match: PME3_PHAVU (Pectinesterase 3 OS=Phaseolus vulgaris GN=MPE3 PE=2 SV=1)

HSP 1 Score: 75.9 bits (185), Expect = 5.6e-13
Identity = 55/177 (31.07%), Postives = 86/177 (48.59%), Query Frame = 1 

Query: 25  THSAQTSYVQEACSVTLHPDLCIRSLSPFSSTAKRSPTKWARASVSVTITEAKAVARFLV 84
           T  +  + ++  C  T +P  C  S+S    +    P    + S+ V I E   ++ F  
Sbjct: 69  TELSPAASLKAVCDTTRYPSSCFSSISSLPESNTTDPELLFKLSLRVAIDE---LSSFPS 128

Query: 85  RLKNNRRMKGRNRAAVSDCVEVFEDAIDELHRSLGVLRRLS-RSKFDAQMGDLTTWVSAA 144
           +L+ N     R + A+  C  VF DA+D L+ S+  L  ++ R    A + ++ TW+SAA
Sbjct: 129 KLRANAEQDARLQKAIDVCSSVFGDALDRLNDSISALGTVAGRIASSASVSNVETWLSAA 188

Query: 145 LTDEDTCVDGF-----EGERGRLVNLIRNRVVKATYITSNALALVNKLAA--SGFET 194
           LTD+DTC+D          RG L   I   +  +T   SN+LA+V K+    S FET
Sbjct: 189 LTDQDTCLDAVGELNSTAARGALQE-IETAMRNSTEFASNSLAIVTKILGLLSRFET 241

[Close]
BLAST of Cp4.1LG17g06970 vs. Swiss-Prot
Match: PMEU1_SOLLC (Pectinesterase/pectinesterase inhibitor U1 OS=Solanum lycopersicum GN=PMEU1 PE=2 SV=1)

HSP 1 Score: 73.6 bits (179), Expect = 2.8e-12
Identity = 49/164 (29.88%), Postives = 90/164 (54.88%), Query Frame = 1 

Query: 27  SAQTSYVQEACSVTLHPDLCIRSLSPFSSTAKRSPTKWARASVSVTITEAKAVAR--FLV 86
           S+  + V+ ACS TLHP+LC  ++   S  +K+  ++     +S+ IT  KAV R  + V
Sbjct: 60  SSAHAIVKSACSNTLHPELCYSAIVNVSDFSKKVTSQKDVIELSLNIT-VKAVRRNYYAV 119

Query: 87  R--LKNNRRMKGRNRAAVSDCVEVFEDAIDELHRSLGVLRRLSRSK-FDAQMGDLTTWVS 146
           +  +K  + +  R + A+ DC+E  ++ +DELH ++  L      K     + DL T +S
Sbjct: 120 KELIKTRKGLTPREKVALHDCLETMDETLDELHTAVEDLELYPNKKSLKEHVEDLKTLIS 179

Query: 147 AALTDEDTCVDGFEGERG--RLVNLIRNRVVKATYITSNALALV 184
           +A+T+++TC+DGF  +    ++  ++         + SNALA++
Sbjct: 180 SAITNQETCLDGFSHDEADKKVRKVLLKGQKHVEKMCSNALAMI 222

[Close]
BLAST of Cp4.1LG17g06970 vs. Swiss-Prot
Match: PME3_ARATH (Pectinesterase/pectinesterase inhibitor 3 OS=Arabidopsis thaliana GN=PME3 PE=2 SV=2)

HSP 1 Score: 71.6 bits (174), Expect = 1.1e-11
Identity = 45/159 (28.30%), Postives = 84/159 (52.83%), Query Frame = 1 

Query: 33  VQEACSVTLHPDLCIRSLSPFSSTAKRSPTKWARASVSVTITEAKAVARFLVR--LKNNR 92
           ++ +CS T +P+LCI ++         S      ASV++TIT  +    F V+  +K  +
Sbjct: 59  LRSSCSSTRYPELCISAVVTAGGVELTSQKDVIEASVNLTITAVEH-NYFTVKKLIKKRK 118

Query: 93  RMKGRNRAAVSDCVEVFEDAIDELHRSLGVLRRLSRSK-FDAQMGDLTTWVSAALTDEDT 152
            +  R + A+ DC+E  ++ +DELH ++  L      K      GDL T +S+A+T+++T
Sbjct: 119 GLTPREKTALHDCLETIDETLDELHETVEDLHLYPTKKTLREHAGDLKTLISSAITNQET 178

Query: 153 CVDGFEGERG--RLVNLIRNRVVKATYITSNALALVNKL 187
           C+DGF  +    ++   +    +   ++ SNALA++  +
Sbjct: 179 CLDGFSHDDADKQVRKALLKGQIHVEHMCSNALAMIKNM 216

[Close]
BLAST of Cp4.1LG17g06970 vs. Swiss-Prot
Match: PME61_ARATH (Probable pectinesterase/pectinesterase inhibitor 61 OS=Arabidopsis thaliana GN=PME61 PE=2 SV=1)

HSP 1 Score: 71.6 bits (174), Expect = 1.1e-11
Identity = 46/156 (29.49%), Postives = 79/156 (50.64%), Query Frame = 1 

Query: 30  TSYVQEACSVTLHPDLCIRSLSPFSSTAKRSPTKWARASVSVTITEAKAVARFLVRLKNN 89
           T  +   CS +L+P+LCI +L  F  +      +    S + T+ +    A +       
Sbjct: 72  TQAISRTCSKSLYPNLCIDTLLDFPGSLTADENELIHISFNATLQKFSK-ALYTSSTITY 131

Query: 90  RRMKGRNRAAVSDCVEVFEDAIDELHRSLGVLRRLSRSKFDAQMGDLTTWVSAALTDEDT 149
            +M  R R+A   C+E+ +D++D L R+L  +  +S    D    D+ TW+S+A+T+ DT
Sbjct: 132 TQMPPRVRSAYDSCLELLDDSVDALTRALSSVVVVSG---DESHSDVMTWLSSAMTNHDT 191

Query: 150 CVDGF---EGERGRLVNLIRNRVVKATYITSNALAL 183
           C DGF   EG+ G + + +   V   + + SN LA+
Sbjct: 192 CTDGFDEIEGQGGEVKDQVIGAVKDLSEMVSNCLAI 223

[Close]
BLAST of Cp4.1LG17g06970 vs. TrEMBL
Match: A0A0A0KAX0_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_7G397050 PE=4 SV=1)

HSP 1 Score: 313.9 bits (803), Expect = 1.4e-82
Identity = 163/197 (82.74%), Postives = 173/197 (87.82%), Query Frame = 1 

Query: 1   MLRAPTSRTSIVTLLAFISILPWLTHSAQTSYVQEACSVTLHPDLCIRSLSPFSSTAKRS 60
           MLRAP+ R SI+ LLA ISILPWLTHSA+TSYVQEAC VT H DLCI+SLSPFSS AKRS
Sbjct: 1   MLRAPSRRASIIALLALISILPWLTHSAKTSYVQEACRVTRHQDLCIQSLSPFSSAAKRS 60

Query: 61  PTKWARASVSVTITEAKAVARFLVRLKNNRRMKGRNRAAVSDCVEVFEDAIDELHRSLGV 120
           PTKWARA VSVTITEAK VA  L RLKNN+RMKGRNRAAV DCVEVFE AIDELHRSLGV
Sbjct: 61  PTKWARAGVSVTITEAKKVAGLLGRLKNNKRMKGRNRAAVLDCVEVFEAAIDELHRSLGV 120

Query: 121 LRRLSRSKFDAQMGDLTTWVSAALTDEDTCVDGFEGERGRLVNLIRNRVVKATYITSNAL 180
           LRRLSR  FDAQMGDLTTWVSAALTDEDTCV+GFEGE G++V L+RNRVVK  YITSNAL
Sbjct: 121 LRRLSRRNFDAQMGDLTTWVSAALTDEDTCVEGFEGEEGKVVTLLRNRVVKVGYITSNAL 180

Query: 181 ALVNKLAASGFETAPNL 198
           ALVNKLAAS FET  N+
Sbjct: 181 ALVNKLAASSFETTINM 197

[Close]
BLAST of Cp4.1LG17g06970 vs. TrEMBL
Match: I3SDE4_LOTJA (Uncharacterized protein OS=Lotus japonicus PE=2 SV=1)

HSP 1 Score: 218.4 bits (555), Expect = 7.8e-54
Identity = 117/189 (61.90%), Postives = 140/189 (74.07%), Query Frame = 1 

Query: 14  LLAFISILPWLTHSAQT-----------SYVQEACSVTLHPDLCIRSLSPFSSTAKRSPT 73
           LL    +LP L ++ Q            S V+EACSVT + +LCI +LS FS+TA  SP+
Sbjct: 6   LLLITLLLPLLANTGQNQMVFAKGKNNNSNVREACSVTRYQNLCIHTLSHFSNTAGTSPS 65

Query: 74  KWARASVSVTITEAKAVARFLVRLKNNRRM-KGRNRAAVSDCVEVFEDAIDELHRSLGVL 133
           KWARA VSVTITE K V  +L +LK N+RM KGRNRAA+SDCVE F DA+DELHRSLGVL
Sbjct: 66  KWARAGVSVTITEVKNVQAYLTKLKKNKRMMKGRNRAALSDCVECFADALDELHRSLGVL 125

Query: 134 RRLSRSKFDAQMGDLTTWVSAALTDEDTCVDGFEGERGRLVNLIRNRVVKATYITSNALA 191
           RRLS+  F  QMGDL TW+SAALTDEDTC+DGFEG+  R + L++NRV   +YITSNALA
Sbjct: 126 RRLSKKTFSTQMGDLNTWISAALTDEDTCIDGFEGKTERQIKLLQNRVQNVSYITSNALA 185

[Close]
BLAST of Cp4.1LG17g06970 vs. TrEMBL
Match: A0A0D2T616_GOSRA (Uncharacterized protein OS=Gossypium raimondii GN=B456_008G145300 PE=4 SV=1)

HSP 1 Score: 217.2 bits (552), Expect = 1.7e-53
Identity = 106/183 (57.92%), Postives = 142/183 (77.60%), Query Frame = 1 

Query: 12  VTLLAFISILPWL--THSAQTSYVQEACSVTLHPDLCIRSLSPFSSTAKRSPTKWARASV 71
           +TLL  +S+L W+  T  + T+YVQ+ACSVT +  LCI +L+PFSSTAK SP+KWARA V
Sbjct: 10  LTLLLLLSVLKWVQSTPHSSTNYVQDACSVTRYRALCINTLAPFSSTAKTSPSKWARAGV 69

Query: 72  SVTITEAKAVARFLVRLKNNRRMKGRNRAAVSDCVEVFEDAIDELHRSLGVLRRLSRSKF 131
           S+TI E K V ++L+++KN R MKG  +  +SDC+E  +DAID+LH SLGVLR+L+   F
Sbjct: 70  SITIGETKKVTQYLMKVKNYRTMKGSYKIPLSDCIECLQDAIDQLHGSLGVLRKLNARNF 129

Query: 132 DAQMGDLTTWVSAALTDEDTCVDGFEGERGRLVNLIRNRVVKATYITSNALALVNKLAAS 191
            AQMGD+TTW+SA LTD +TC+DGFE  RG+   ++RNRV++++Y TSNALALVNKLA S
Sbjct: 130 YAQMGDITTWLSAVLTDHETCLDGFENPRGKQAKMVRNRVLRSSYFTSNALALVNKLATS 189

Query: 192 GFE 193
           G +
Sbjct: 190 GLD 192

[Close]
BLAST of Cp4.1LG17g06970 vs. TrEMBL
Match: M5VKL3_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa016389mg PE=4 SV=1)

HSP 1 Score: 216.9 bits (551), Expect = 2.3e-53
Identity = 109/183 (59.56%), Postives = 149/183 (81.42%), Query Frame = 1 

Query: 15  LAFISILPWLTHSAQTSYVQEACSVTLHPDLCIRSLSPFSSTAKRSPTKWARASVSVTIT 74
           L  I+ +  ++ +   + VQ+ACSVT + DLCI SL+ FSS+AK SP++WARA+VSVT+ 
Sbjct: 3   LCLINSVQLVSGNGNQNNVQKACSVTRYQDLCIHSLASFSSSAKSSPSRWARAAVSVTLG 62

Query: 75  EAKAVARFLVRLKNNRRMKGR-NRAAVSDCVEVFEDAIDELHRSLGVLRRLSRSKFDAQM 134
           EAK+VA++L+ L+ +  + GR +R A+SDC+E F++AIDELH+SLGVLR LSR+ FD QM
Sbjct: 63  EAKSVAQYLLALQKHSGINGRRSRVALSDCIECFQNAIDELHKSLGVLRSLSRTTFDTQM 122

Query: 135 GDLTTWVSAALTDEDTCVDGFEGERGRLVNLIRNRVVKATYITSNALALVNKLAASGFET 194
           GDL TW+SAALT+ DTC+DGFEG+RGR V L++N+V+KAT+ITSNALAL NKLAA+G E+
Sbjct: 123 GDLNTWLSAALTNGDTCLDGFEGQRGRQVKLLQNKVLKATHITSNALALANKLAATGLES 182

Query: 195 APN 197
            P+
Sbjct: 183 LPH 185

[Close]
BLAST of Cp4.1LG17g06970 vs. TrEMBL
Match: B9I9R5_POPTR (Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0014s12390g PE=4 SV=1)

HSP 1 Score: 214.5 bits (545), Expect = 1.1e-52
Identity = 109/185 (58.92%), Postives = 140/185 (75.68%), Query Frame = 1 

Query: 12  VTLLAFISILPWLTHSAQT------SYVQEACSVTLHPDLCIRSLSPFSSTAKRSPTKWA 71
           +++L     L WLT +         +YV+EACSVT + DLC+ SL+ FS TA RSP+KWA
Sbjct: 4   ISILITFLFLSWLTCATSRGSDNGDTYVREACSVTRYHDLCMHSLASFSHTAGRSPSKWA 63

Query: 72  RASVSVTITEAKAVARFLVRLKNNRRMKGRNRAAVSDCVEVFEDAIDELHRSLGVLRRLS 131
           RA VSVTI EAK  +++L +LK +R M+GRNR A+SDC+E F+DAID LH+SLG+LR+L 
Sbjct: 64  RAGVSVTIGEAKNASQYLNKLKKDRIMRGRNRIALSDCIECFQDAIDNLHKSLGILRKLD 123

Query: 132 RSKFDAQMGDLTTWVSAALTDEDTCVDGFEGERGRLVNLIRNRVVKATYITSNALALVNK 191
            + FD QMGDLTTW+SAALTDEDTC+DGFE    + V ++ N+V + TYITSNALALVNK
Sbjct: 124 ATNFDTQMGDLTTWLSAALTDEDTCLDGFEDRSSKQVKMLLNQVSRVTYITSNALALVNK 183

[Close]
BLAST of Cp4.1LG17g06970 vs. TAIR10
Match: AT3G62820.1 (AT3G62820.1 Plant invertase/pectin methylesterase inhibitor superfamily protein)

HSP 1 Score: 168.7 bits (426), Expect = 3.6e-42
Identity = 96/194 (49.48%), Postives = 134/194 (69.07%), Query Frame = 1 

Query: 2   LRAPTSRTSIVTLLAFI-SILPWLT-HSAQTSYVQEACSVTLHPDLCIRSLSPFSSTAKR 61
           ++ P S +    L+ F+ S+ P  +  S + SYVQ ACSVT + DLC ++L PF+S AK 
Sbjct: 1   MKTPMSSSITFALVFFLLSLNPTSSLPSKRESYVQNACSVTRYQDLCAKTLLPFASVAKN 60

Query: 62  SPTKWARASVSVTITEAKAVARFLVRLKNNRRMKGRNRAAVSDCVEVFEDAIDELHRSLG 121
           SP+KWARA VSV IT+ K V R L++ + +   K R+R A+SDC E+ +D++D LH+SL 
Sbjct: 61  SPSKWARAGVSVAITDNKDVLRHLLKTRLSTIGK-RDRIALSDCRELLQDSLDSLHKSLA 120

Query: 122 VLRRLSRSKFDAQMGDLTTWVSAALTDEDTCVDGFEGERGR---LVNLIRNRVVKATYIT 181
           VLR L  S+F  QM DL TW+S++LTD+DTC+DGFE    R    V +IR RV  + Y++
Sbjct: 121 VLRTLRASEFQQQMSDLATWLSSSLTDKDTCLDGFEKTSTRSSSTVRMIRKRVTTSMYLS 180

Query: 182 SNALALVNKLAASG 191
           SN+LAL+NKLAA+G
Sbjct: 181 SNSLALLNKLAANG 193

[Close]
BLAST of Cp4.1LG17g06970 vs. TAIR10
Match: AT2G47670.1 (AT2G47670.1 Plant invertase/pectin methylesterase inhibitor superfamily protein)

HSP 1 Score: 153.3 bits (386), Expect = 1.6e-37
Identity = 82/164 (50.00%), Postives = 113/164 (68.90%), Query Frame = 1 

Query: 30  TSYVQEACSVTLHPDLCIRSLSPFSSTAKRSPTKWARASVSVTITEAKAVARFLVRLKNN 89
           ++YV+ AC+VT +  LC+R+L PF+  A+ + +KWARASV+VTIT+ K V R L  LK  
Sbjct: 46  STYVRNACNVTRYNRLCVRTLWPFAIVARNNTSKWARASVAVTITDTKRVLRLL--LKTQ 105

Query: 90  RRMKGRN-RAAVSDCVEVFEDAIDELHRSLGVLRRLSRSKFDAQMGDLTTWVSAALTDED 149
           R   G + R A+SDC E+F D++D L++SL VLR L+  +F  Q+ DL TW+SAALTD+D
Sbjct: 106 RSAVGESERIALSDCRELFVDSLDNLYKSLAVLRTLNADEFQRQISDLATWLSAALTDDD 165

Query: 150 TCVDGFE--GERGRLVNLIRNRVVKATYITSNALALVNKLAASG 191
           TC+DGFE    R R V ++R +  K   + SNALAL+ KLA  G
Sbjct: 166 TCLDGFEETSSRTRTVRMVRRKATKCMRLCSNALALLKKLAFDG 207

[Close]
BLAST of Cp4.1LG17g06970 vs. TAIR10
Match: AT5G62360.1 (AT5G62360.1 Plant invertase/pectin methylesterase inhibitor superfamily protein)

HSP 1 Score: 135.6 bits (340), Expect = 3.4e-32
Identity = 69/166 (41.57%), Postives = 109/166 (65.66%), Query Frame = 1 

Query: 25  THSAQTSYVQEACSVTLHPDLCIRSLSPFSSTAKRSPTKWARASVSVTITEAKAVARFLV 84
           T +  T +V+ +C+ T +P LC  SLS  +S  + SP   A A++++T+  AK  +  +V
Sbjct: 36  TTTTNTEFVKSSCTFTTYPRLCFSSLSTHASLIQTSPKLMAHAALNITLASAKVTSAMMV 95

Query: 85  RLKNNRRMKGRNRAAVSDCVEVFEDAIDELHRSLGVLRRLSRSKFDAQMGDLTTWVSAAL 144
           RL N+R +K +  +A+ DCVE   D ++EL +S+G + +LS S ++  + D+ TWVSAAL
Sbjct: 96  RLSNSR-LKPKEVSAMRDCVEELGDTLEELRKSIGEMCQLSGSNYEVYISDIQTWVSAAL 155

Query: 145 TDEDTCVDGFEGE--RGRLVNLIRNRVVKATYITSNALALVNKLAA 189
           TD +TC DGFEGE   G++  L+R R++   ++TSNALAL+N  A+
Sbjct: 156 TDVNTCTDGFEGEDMDGKVKVLVRGRILVIAHLTSNALALINHFAS 200

[Close]
BLAST of Cp4.1LG17g06970 vs. TAIR10
Match: AT1G62760.1 (AT1G62760.1 Plant invertase/pectin methylesterase inhibitor superfamily protein)

HSP 1 Score: 120.9 bits (302), Expect = 8.6e-28
Identity = 63/170 (37.06%), Postives = 110/170 (64.71%), Query Frame = 1 

Query: 25  THSAQTS--YVQEACSVTLHPDLCIRSLSPFSSTAKRSPTKWARASVSVTITEAKAVARF 84
           T+S QT+  Y++ +C++TL+  +C  SLSP++ST + +P K A  ++++T++ AK+ ++F
Sbjct: 138 TYSNQTNLDYIKTSCNITLYKTICYNSLSPYASTIRSNPQKLAVIALNLTLSSAKSASKF 197

Query: 85  LVRLKNNRRMKGRNRAAVSDCVEVFEDAIDELHRSLGVLRRLS---RSKFDAQMGDLTTW 144
           +  + +   +      AV+DCVE   D++  L  S+  L  ++    +KF+  M D+ TW
Sbjct: 198 VKNISHGGGLTRLEVVAVADCVEEIGDSVTSLQDSIRELDSINYKDSAKFEMVMSDVETW 257

Query: 145 VSAALTDEDTCVDGFEGERGRLVNLIRNRVVKATYITSNALALVNKLAAS 190
           VSAALT++DTC+DGF   +  + +L+R  VV+   +TSNALAL+N  A++
Sbjct: 258 VSAALTNDDTCMDGFSLVKTAVKDLVRRHVVEVARLTSNALALINMYAST 307

[Close]
BLAST of Cp4.1LG17g06970 vs. TAIR10
Match: AT5G62350.1 (AT5G62350.1 Plant invertase/pectin methylesterase inhibitor superfamily protein)

HSP 1 Score: 117.1 bits (292), Expect = 1.2e-26
Identity = 61/163 (37.42%), Postives = 97/163 (59.51%), Query Frame = 1 

Query: 31  SYVQEACSVTLHPDLCIRSLSPFSSTAKRSPTKWARASVSVTITEAKAVARFLVRLKNNR 90
           +++Q +C  T +P LC+ SLS +++  + SP + A  +++VT++ A++   F+ RL   +
Sbjct: 37  NFIQSSCKTTTYPALCVHSLSVYANDIQTSPKRLAETAIAVTLSRAQSTKLFVSRLTRMK 96

Query: 91  RMKGRNRAAVSDCVEVFEDAIDELHRSLGVLRRLSRSK----FDAQMGDLTTWVSAALTD 150
            +K R   A+ DCVE   D +D L +S+  L+    +K    F   M +  TW SAALTD
Sbjct: 97  GLKKREVEAIKDCVEEMNDTVDRLTKSVQELKLCGSAKDQDQFAYHMSNAQTWTSAALTD 156

Query: 151 EDTCVDGFEGE--RGRLVNLIRNRVVKATYITSNALALVNKLA 188
           E+TC DGF G    GR+ N +R R++   + TSNAL+L+N  A
Sbjct: 157 ENTCSDGFSGRVMDGRIKNSVRARIMNVGHETSNALSLINAFA 199

[Close]
BLAST of Cp4.1LG17g06970 vs. NCBI nr
Match: gi|659101359|ref|XP_008451567.1| (PREDICTED: 21 kDa protein [Cucumis melo])

HSP 1 Score: 317.8 bits (813), Expect = 1.4e-83
Identity = 163/197 (82.74%), Postives = 177/197 (89.85%), Query Frame = 1 

Query: 1   MLRAPTSRTSIVTLLAFISILPWLTHSAQTSYVQEACSVTLHPDLCIRSLSPFSSTAKRS 60
           M+R P++R SI  LLA ISILPWLTHSA+TSYVQEAC VTLH DLCI+SLSPFSSTAKRS
Sbjct: 1   MMRPPSTRASIAALLALISILPWLTHSAKTSYVQEACRVTLHQDLCIQSLSPFSSTAKRS 60

Query: 61  PTKWARASVSVTITEAKAVARFLVRLKNNRRMKGRNRAAVSDCVEVFEDAIDELHRSLGV 120
           PTKWARA VSVTITEAK VA  L RLKNN+RMKGRNRAAV DCVEVF+ AIDELHRSLGV
Sbjct: 61  PTKWARAGVSVTITEAKKVAALLGRLKNNKRMKGRNRAAVLDCVEVFDAAIDELHRSLGV 120

Query: 121 LRRLSRSKFDAQMGDLTTWVSAALTDEDTCVDGFEGERGRLVNLIRNRVVKATYITSNAL 180
           LRRLSR  FD+QMGDLTTWVSAALTDEDTCV+GFEGERG++V+L+RNRVVK  YITSNAL
Sbjct: 121 LRRLSRRNFDSQMGDLTTWVSAALTDEDTCVEGFEGERGKVVSLLRNRVVKVGYITSNAL 180

Query: 181 ALVNKLAASGFETAPNL 198
           ALVNKLA SGFETA N+
Sbjct: 181 ALVNKLATSGFETALNM 197

[Close]
BLAST of Cp4.1LG17g06970 vs. NCBI nr
Match: gi|778728057|ref|XP_011659361.1| (PREDICTED: 21 kDa protein [Cucumis sativus])

HSP 1 Score: 313.9 bits (803), Expect = 2.0e-82
Identity = 163/197 (82.74%), Postives = 173/197 (87.82%), Query Frame = 1 

Query: 1   MLRAPTSRTSIVTLLAFISILPWLTHSAQTSYVQEACSVTLHPDLCIRSLSPFSSTAKRS 60
           MLRAP+ R SI+ LLA ISILPWLTHSA+TSYVQEAC VT H DLCI+SLSPFSS AKRS
Sbjct: 1   MLRAPSRRASIIALLALISILPWLTHSAKTSYVQEACRVTRHQDLCIQSLSPFSSAAKRS 60

Query: 61  PTKWARASVSVTITEAKAVARFLVRLKNNRRMKGRNRAAVSDCVEVFEDAIDELHRSLGV 120
           PTKWARA VSVTITEAK VA  L RLKNN+RMKGRNRAAV DCVEVFE AIDELHRSLGV
Sbjct: 61  PTKWARAGVSVTITEAKKVAGLLGRLKNNKRMKGRNRAAVLDCVEVFEAAIDELHRSLGV 120

Query: 121 LRRLSRSKFDAQMGDLTTWVSAALTDEDTCVDGFEGERGRLVNLIRNRVVKATYITSNAL 180
           LRRLSR  FDAQMGDLTTWVSAALTDEDTCV+GFEGE G++V L+RNRVVK  YITSNAL
Sbjct: 121 LRRLSRRNFDAQMGDLTTWVSAALTDEDTCVEGFEGEEGKVVTLLRNRVVKVGYITSNAL 180

Query: 181 ALVNKLAASGFETAPNL 198
           ALVNKLAAS FET  N+
Sbjct: 181 ALVNKLAASSFETTINM 197

[Close]
BLAST of Cp4.1LG17g06970 vs. NCBI nr
Match: gi|1009148446|ref|XP_015891941.1| (PREDICTED: 21 kDa protein-like [Ziziphus jujuba])

HSP 1 Score: 220.7 bits (561), Expect = 2.3e-54
Identity = 109/165 (66.06%), Postives = 135/165 (81.82%), Query Frame = 1 

Query: 27  SAQTSYVQEACSVTLHPDLCIRSLSPFSSTAKRSPTKWARASVSVTITEAKAVARFLVRL 86
           + +  YV++AC VT +PDLCI SL+PFS+TAK SPT WARA VSVT++EAK+VAR+LVRL
Sbjct: 28  NGKNKYVRDACRVTRYPDLCIHSLAPFSNTAKTSPTLWARAGVSVTLSEAKSVARYLVRL 87

Query: 87  KNNRR-MKGRNRAAVSDCVEVFEDAIDELHRSLGVLRRLSRSKFDAQMGDLTTWVSAALT 146
           K+ R   +GRN AA+SDC+E FE+ IDELH+SL VLR LSR  F  QM DL TW+SAALT
Sbjct: 88  KSQRSSFRGRNSAALSDCIECFENVIDELHKSLRVLRSLSRGTFSTQMADLNTWLSAALT 147

Query: 147 DEDTCVDGFEGERGRLVNLIRNRVVKATYITSNALALVNKLAASG 191
           DEDTC+DGF G++G+L+  + NRV KA+YITSNALAL NKLAA+G
Sbjct: 148 DEDTCLDGFAGQKGKLIKFLLNRVSKASYITSNALALANKLAATG 192

[Close]
BLAST of Cp4.1LG17g06970 vs. NCBI nr
Match: gi|388500440|gb|AFK38286.1| (unknown [Lotus japonicus])

HSP 1 Score: 218.4 bits (555), Expect = 1.1e-53
Identity = 117/189 (61.90%), Postives = 140/189 (74.07%), Query Frame = 1 

Query: 14  LLAFISILPWLTHSAQT-----------SYVQEACSVTLHPDLCIRSLSPFSSTAKRSPT 73
           LL    +LP L ++ Q            S V+EACSVT + +LCI +LS FS+TA  SP+
Sbjct: 6   LLLITLLLPLLANTGQNQMVFAKGKNNNSNVREACSVTRYQNLCIHTLSHFSNTAGTSPS 65

Query: 74  KWARASVSVTITEAKAVARFLVRLKNNRRM-KGRNRAAVSDCVEVFEDAIDELHRSLGVL 133
           KWARA VSVTITE K V  +L +LK N+RM KGRNRAA+SDCVE F DA+DELHRSLGVL
Sbjct: 66  KWARAGVSVTITEVKNVQAYLTKLKKNKRMMKGRNRAALSDCVECFADALDELHRSLGVL 125

Query: 134 RRLSRSKFDAQMGDLTTWVSAALTDEDTCVDGFEGERGRLVNLIRNRVVKATYITSNALA 191
           RRLS+  F  QMGDL TW+SAALTDEDTC+DGFEG+  R + L++NRV   +YITSNALA
Sbjct: 126 RRLSKKTFSTQMGDLNTWISAALTDEDTCIDGFEGKTERQIKLLQNRVQNVSYITSNALA 185

[Close]
BLAST of Cp4.1LG17g06970 vs. NCBI nr
Match: gi|743870288|ref|XP_011033536.1| (PREDICTED: 21 kDa protein [Populus euphratica])

HSP 1 Score: 217.6 bits (553), Expect = 1.9e-53
Identity = 109/185 (58.92%), Postives = 142/185 (76.76%), Query Frame = 1 

Query: 12  VTLLAFISILPWLTHSAQ------TSYVQEACSVTLHPDLCIRSLSPFSSTAKRSPTKWA 71
           +++L     L WLT +         +YV+EACSVT + DLC+ SL+ FS TA RSP+KWA
Sbjct: 4   ISILIIFLFLSWLTCATSWGSDNGDTYVREACSVTRYHDLCMHSLASFSHTAGRSPSKWA 63

Query: 72  RASVSVTITEAKAVARFLVRLKNNRRMKGRNRAAVSDCVEVFEDAIDELHRSLGVLRRLS 131
           RA VSVTI EAK  +++L +LK +  M+GRNR A+SDC+E F+DA+D LHRSLG+LR+L 
Sbjct: 64  RAGVSVTIGEAKNASQYLNKLKKDGIMRGRNRIALSDCIECFQDAMDNLHRSLGILRKLD 123

Query: 132 RSKFDAQMGDLTTWVSAALTDEDTCVDGFEGERGRLVNLIRNRVVKATYITSNALALVNK 191
            + FD QMGDLTTW+SAALTDEDTC+DGFE +  + VN+++N+V + TYITSNALALVNK
Sbjct: 124 ATNFDTQMGDLTTWLSAALTDEDTCLDGFEDQSSKQVNMLQNQVTRVTYITSNALALVNK 183

The following BLAST results are available for this feature:
BLAST of Cp4.1LG17g06970 vs. Swiss-Prot
Match NameE-valueIdentityDescription
21KD_DAUCA4.0e-2736.4121 kDa protein OS=Daucus carota PE=2 SV=1[more]
PME3_PHAVU5.6e-1331.07Pectinesterase 3 OS=Phaseolus vulgaris GN=MPE3 PE=2 SV=1[more]
PMEU1_SOLLC2.8e-1229.88Pectinesterase/pectinesterase inhibitor U1 OS=Solanum lycopersicum GN=PMEU1 PE=2... [more]
PME3_ARATH1.1e-1128.30Pectinesterase/pectinesterase inhibitor 3 OS=Arabidopsis thaliana GN=PME3 PE=2 S... [more]
PME61_ARATH1.1e-1129.49Probable pectinesterase/pectinesterase inhibitor 61 OS=Arabidopsis thaliana GN=P... [more]
BLAST of Cp4.1LG17g06970 vs. TrEMBL
Match NameE-valueIdentityDescription
A0A0A0KAX0_CUCSA1.4e-8282.74Uncharacterized protein OS=Cucumis sativus GN=Csa_7G397050 PE=4 SV=1[more]
I3SDE4_LOTJA7.8e-5461.90Uncharacterized protein OS=Lotus japonicus PE=2 SV=1[more]
A0A0D2T616_GOSRA1.7e-5357.92Uncharacterized protein OS=Gossypium raimondii GN=B456_008G145300 PE=4 SV=1[more]
M5VKL3_PRUPE2.3e-5359.56Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa016389mg PE=4 SV=1[more]
B9I9R5_POPTR1.1e-5258.92Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0014s12390g PE=4 SV=1[more]
BLAST of Cp4.1LG17g06970 vs. TAIR10
Match NameE-valueIdentityDescription
AT3G62820.13.6e-4249.48 Plant invertase/pectin methylesterase inhibitor superfamily protein[more]
AT2G47670.11.6e-3750.00 Plant invertase/pectin methylesterase inhibitor superfamily protein[more]
AT5G62360.13.4e-3241.57 Plant invertase/pectin methylesterase inhibitor superfamily protein[more]
AT1G62760.18.6e-2837.06 Plant invertase/pectin methylesterase inhibitor superfamily protein[more]
AT5G62350.11.2e-2637.42 Plant invertase/pectin methylesterase inhibitor superfamily protein[more]
BLAST of Cp4.1LG17g06970 vs. NCBI nr
Match NameE-valueIdentityDescription
gi|659101359|ref|XP_008451567.1|1.4e-8382.74PREDICTED: 21 kDa protein [Cucumis melo][more]
gi|778728057|ref|XP_011659361.1|2.0e-8282.74PREDICTED: 21 kDa protein [Cucumis sativus][more]
gi|1009148446|ref|XP_015891941.1|2.3e-5466.06PREDICTED: 21 kDa protein-like [Ziziphus jujuba][more]
gi|388500440|gb|AFK38286.1|1.1e-5361.90unknown [Lotus japonicus][more]
gi|743870288|ref|XP_011033536.1|1.9e-5358.92PREDICTED: 21 kDa protein [Populus euphratica][more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: Molecular Function
TermDefinition
GO:0004857enzyme inhibitor activity
Vocabulary: INTERPRO
TermDefinition
IPR006501Pectinesterase_inhib_dom
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0043086 negative regulation of catalytic activity
biological_process GO:0005982 starch metabolic process
biological_process GO:0005985 sucrose metabolic process
biological_process GO:0008150 biological_process
cellular_component GO:0005575 cellular_component
molecular_function GO:0004857 enzyme inhibitor activity
molecular_function GO:0030599 pectinesterase activity
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG17g06970.1Cp4.1LG17g06970.1mRNA


InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo
Date Performed: 2017-12-02
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006501Pectinesterase inhibitor domainGENE3DG3DSA:1.20.140.40coord: 29..186
score: 1.5
IPR006501Pectinesterase inhibitor domainPFAMPF04043PMEIcoord: 32..182
score: 6.7
IPR006501Pectinesterase inhibitor domainSMARTSM00856PMEI_2coord: 28..182
score: 8.4
IPR006501Pectinesterase inhibitor domainTIGRFAMsTIGR01614TIGR01614coord: 10..186
score: 4.1
IPR006501Pectinesterase inhibitor domainunknownSSF101148Plant invertase/pectin methylesterase inhibitorcoord: 31..186
score: 1.57
NoneNo IPR availablePANTHERPTHR31080FAMILY NOT NAMEDcoord: 1..195
score: 2.3
NoneNo IPR availablePANTHERPTHR31080:SF16SUBFAMILY NOT NAMEDcoord: 1..195
score: 2.3

Synteny
The following gene(s) are orthologous to this gene:
GeneOrthologueOrganismBlock
Cp4.1LG17g06970Cucsa.205560Cucumber (Gy14) v1cgycpeB0580
Cp4.1LG17g06970CmaCh17G010870Cucurbita maxima (Rimu)cmacpeB376
Cp4.1LG17g06970CmaCh08G004230Cucurbita maxima (Rimu)cmacpeB917
Cp4.1LG17g06970CmoCh08G004170Cucurbita moschata (Rifu)cmocpeB853
Cp4.1LG17g06970Cla015069Watermelon (97103) v1cpewmB311
Cp4.1LG17g06970Csa7G397050Cucumber (Chinese Long) v2cpecuB343
Cp4.1LG17g06970MELO3C016001Melon (DHL92) v3.5.1cpemeB293
Cp4.1LG17g06970ClCG09G005870Watermelon (Charleston Gray)cpewcgB280
Cp4.1LG17g06970CSPI07G17480Wild cucumber (PI 183967)cpecpiB344
Cp4.1LG17g06970Lsi02G024050Bottle gourd (USVL1VR-Ls)cpelsiB262
Cp4.1LG17g06970MELO3C016001.2Melon (DHL92) v3.6.1cpemedB341
Cp4.1LG17g06970CsaV3_7G029270Cucumber (Chinese Long) v3cpecucB0422
Cp4.1LG17g06970Bhi09G000717Wax gourdcpewgoB0420
Cp4.1LG17g06970CsGy7G015920Cucumber (Gy14) v2cgybcpeB931
Cp4.1LG17g06970Carg12101Silver-seed gourdcarcpeB0803
Cp4.1LG17g06970Carg07171Silver-seed gourdcarcpeB0161
The following gene(s) are paralogous to this gene:
GeneParalogueOrganismBlock
Cp4.1LG17g06970Cp4.1LG12g05670Cucurbita pepo (Zucchini)cpecpeB157
The following block(s) are covering this gene:
GeneOrganismBlock
Cp4.1LG17g06970Cucurbita pepo (Zucchini)cpecpeB336
Cp4.1LG17g06970Cucumber (Gy14) v1cgycpeB0189
Cp4.1LG17g06970Cucurbita moschata (Rifu)cmocpeB232
Cp4.1LG17g06970Cucurbita moschata (Rifu)cmocpeB339
Cp4.1LG17g06970Wild cucumber (PI 183967)cpecpiB316
Cp4.1LG17g06970Cucumber (Chinese Long) v2cpecuB315
Cp4.1LG17g06970Melon (DHL92) v3.5.1cpemeB318
Cp4.1LG17g06970Melon (DHL92) v3.6.1cpemedB375
Cp4.1LG17g06970Cucumber (Chinese Long) v3cpecucB0393
Cp4.1LG17g06970Wax gourdcpewgoB0376