Csa6G022340 (gene) Cucumber (Chinese Long) v2

NameCsa6G022340
Typegene
OrganismCucumis. sativus (Cucumber (Chinese Long) v2)
DescriptionCysteine proteinase inhibitor B; contains IPR000010 (Proteinase inhibitor I25, cystatin), IPR027214 (Cystatin)
LocationChr6 : 2289322 .. 2289875 (+)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GAGAATAAGAAATTAGAGAACGCTGGAATTAAGTAATGGCGAAAACAAAATTGAGTGTCATGGTGGTAGTGCTGGCGGTGGCTTTGGTGGCTTTGGTGGAAGGATATGGGGGGAGAGTAGGAGGAAGGATGGAGGTTAAAGACGTGAGGAGGAACGAGGAGGTGCAGCGATTAGGAAGATTCAGCGTGGAGGAGTACAATCGTAGGATGGGAGGCGGGGGAGAGGTGAAGTTCACGGCGGTAGTTGCGGCGGAGAGGCAAGTGGTGTCGGGAACGAAATACTATTTGAGAATTTTGGGGACTCAAAATGGGGAGAGGAAAGTTTTTGATTCGGTTGTGATTGTGAAGCCTTGGATTGGTTCTAAGCGCCTTCTCGACTTTTCTCCTTCCGCAGTTTTTAGAACTCCCATTTTTAATTTTTGATTTCTTTTTTTTTCTTATTTTCTTGGGAAAATGGAGTTTTCTTTTCTTTCTCAGAGTTGTGAAGCAGAAGAGGAGCTGTAAATTTTTCTTCTTTCTTCCCAGTGAGCAATAAGAAATAAGTATGAGTTTAAA

mRNA sequence

ATGGCGAAAACAAAATTGAGTGTCATGGTGGTAGTGCTGGCGGTGGCTTTGGTGGCTTTGGTGGAAGGATATGGGGGGAGAGTAGGAGGAAGGATGGAGGTTAAAGACGTGAGGAGGAACGAGGAGGTGCAGCGATTAGGAAGATTCAGCGTGGAGGAGTACAATCGTAGGATGGGAGGCGGGGGAGAGGTGAAGTTCACGGCGGTAGTTGCGGCGGAGAGGCAAGTGGTGTCGGGAACGAAATACTATTTGAGAATTTTGGGGACTCAAAATGGGGAGAGGAAAGTTTTTGATTCGGTTGTGATTGTGAAGCCTTGGATTGGTTCTAAGCGCCTTCTCGACTTTTCTCCTTCCGCAGTTTTTAGAACTCCCATTTTTAATTTTTGA

Coding sequence (CDS)

ATGGCGAAAACAAAATTGAGTGTCATGGTGGTAGTGCTGGCGGTGGCTTTGGTGGCTTTGGTGGAAGGATATGGGGGGAGAGTAGGAGGAAGGATGGAGGTTAAAGACGTGAGGAGGAACGAGGAGGTGCAGCGATTAGGAAGATTCAGCGTGGAGGAGTACAATCGTAGGATGGGAGGCGGGGGAGAGGTGAAGTTCACGGCGGTAGTTGCGGCGGAGAGGCAAGTGGTGTCGGGAACGAAATACTATTTGAGAATTTTGGGGACTCAAAATGGGGAGAGGAAAGTTTTTGATTCGGTTGTGATTGTGAAGCCTTGGATTGGTTCTAAGCGCCTTCTCGACTTTTCTCCTTCCGCAGTTTTTAGAACTCCCATTTTTAATTTTTGA

Protein sequence

MAKTKLSVMVVVLAVALVALVEGYGGRVGGRMEVKDVRRNEEVQRLGRFSVEEYNRRMGGGGEVKFTAVVAAERQVVSGTKYYLRILGTQNGERKVFDSVVIVKPWIGSKRLLDFSPSAVFRTPIFNF*
BLAST of Csa6G022340 vs. Swiss-Prot
Match: CYT4_ORYSJ (Cysteine proteinase inhibitor 4 OS=Oryza sativa subsp. japonica GN=Os01g0915200 PE=2 SV=1)

HSP 1 Score: 109.4 bits (272), Expect = 3.0e-23
Identity = 60/108 (55.56%), Postives = 73/108 (67.59%), Query Frame = 1

Query: 23  GYGGRVGGRMEVKDVRRNEEVQRLGRFSVEEYNRRMG----GGGEVKFTAVVAAERQVVS 82
           G G RVGGR EV+DV  + EVQ LGRFSVEE+NRR      G   ++F  VVAA+RQVVS
Sbjct: 45  GAGRRVGGRTEVRDVEGDREVQELGRFSVEEHNRRRRSRDCGDVRLEFGRVVAAQRQVVS 104

Query: 83  GTKYYLRI-------LGTQNGERKVFDSVVIVKPWIGSKRLLDFSPSA 120
           G KYYLR+        G   GE +VFD+VV+VKPW+ S+ LL F+P+A
Sbjct: 105 GLKYYLRVAAAEEGAAGQNGGEPRVFDAVVVVKPWLESRTLLTFAPAA 152

BLAST of Csa6G022340 vs. Swiss-Prot
Match: CYTB_HELAN (Cysteine proteinase inhibitor B OS=Helianthus annuus PE=1 SV=1)

HSP 1 Score: 98.2 bits (243), Expect = 6.9e-20
Identity = 50/97 (51.55%), Postives = 70/97 (72.16%), Query Frame = 1

Query: 29  GGRMEVKDVRRNEEVQRLGRFSVEEYNR----RMGGGGEVKFTAVVAAERQVVSGTKYYL 88
           GGR +VK+V+ + E+Q+LG +SV+EYNR    +  G G++KF+ V+AAE QVV+GTKYYL
Sbjct: 3   GGRTKVKNVKTDTEIQQLGSYSVDEYNRLQRTKKTGAGDLKFSQVIAAETQVVAGTKYYL 62

Query: 89  RILG-TQNGERKVFDSVVIVKPWIGSKRLLDFSPSAV 121
           +I   T+ G+ KVFD+ V+V+ W  SK+LL F P+ V
Sbjct: 63  KIEAITKGGKMKVFDAEVVVQSWKHSKKLLGFKPAPV 99

BLAST of Csa6G022340 vs. Swiss-Prot
Match: CYT5_ORYSJ (Cysteine proteinase inhibitor 5 OS=Oryza sativa subsp. japonica GN=Os01g0915401 PE=3 SV=2)

HSP 1 Score: 97.1 bits (240), Expect = 1.5e-19
Identity = 51/100 (51.00%), Postives = 65/100 (65.00%), Query Frame = 1

Query: 23  GYGGRVGGRMEVKDVRRNEEVQRLGRFSVEEYNRRMGG---GGEVKFTAVVAAERQVVSG 82
           G G +VGGR +V+DV  N EVQ LG F V E+NRR G    G  + F+ VVAA+ QVVSG
Sbjct: 44  GRGRKVGGRTDVEDVEGNREVQELGLFCVVEHNRRGGSATRGRGLVFSRVVAAQTQVVSG 103

Query: 83  TKYYLRILGTQNGERKVFDSVVIVKPWIGSKRLLDFSPSA 120
            KYYLRI   +  +  VFD+VV+VK W+ S+ ++ F P+A
Sbjct: 104 IKYYLRIAAQEADDELVFDAVVVVKAWVPSREMVSFVPAA 143

BLAST of Csa6G022340 vs. Swiss-Prot
Match: CYT2_ARATH (Cysteine proteinase inhibitor 2 OS=Arabidopsis thaliana GN=CYS2 PE=2 SV=2)

HSP 1 Score: 89.7 bits (221), Expect = 2.5e-17
Identity = 51/136 (37.50%), Postives = 83/136 (61.03%), Query Frame = 1

Query: 5   KLSVMVVVLAVALVALVEGYGGR-------VGGRMEVKDVRRNEEVQRLGRFSVEEYNRR 64
           K+S+++ +L   ++A+V             +GG+  V ++R N E+Q+LGR+ VE++N++
Sbjct: 6   KVSLVLSLLGFLVIAVVTPSAANPFRKSVVLGGKSGVPNIRTNREIQQLGRYCVEQFNQQ 65

Query: 65  ----MGGGGEV-----------KFTAVVAAERQVVSGTKYYLRILGTQ-NGERKVFDSVV 118
                G  G +           +F+ VV+A++QVV+G KYYLRI  TQ NG  ++FDSVV
Sbjct: 66  AQNEQGNIGSIAKTDTAISNPLQFSRVVSAQKQVVAGLKYYLRIEVTQPNGSTRMFDSVV 125

BLAST of Csa6G022340 vs. Swiss-Prot
Match: CYT10_ORYSJ (Cysteine proteinase inhibitor 10 OS=Oryza sativa subsp. japonica GN=Os04g0350100 PE=2 SV=1)

HSP 1 Score: 88.6 bits (218), Expect = 5.5e-17
Identity = 48/110 (43.64%), Postives = 66/110 (60.00%), Query Frame = 1

Query: 28  VGGRMEVKDVRRNEEVQRLGRFSVEEYNRRMGGGG----------EVKFTAVVAAERQVV 87
           VGGR E++DV  N+ VQ LGRF+V E+NRR+  GG          ++ F  VV A++QVV
Sbjct: 39  VGGRTEIRDVGSNKAVQSLGRFAVAEHNRRLRHGGSGGPADPVPVKLAFARVVEAQKQVV 98

Query: 88  SGTKYYLRIL--------GTQNGERKVFDSVVIVKPWIGSKRLLDFSPSA 120
           SG  YYL++         G   G  +VFD+VV+VK W+ SK L+ F+P++
Sbjct: 99  SGVAYYLKVAASARDPRGGAAAGGDRVFDAVVVVKAWLKSKELVSFTPAS 148

BLAST of Csa6G022340 vs. TrEMBL
Match: A0A0A0KC20_CUCSA (Cysteine proteinase inhibitor OS=Cucumis sativus GN=Csa_6G022340 PE=3 SV=1)

HSP 1 Score: 250.4 bits (638), Expect = 1.2e-63
Identity = 128/128 (100.00%), Postives = 128/128 (100.00%), Query Frame = 1

Query: 1   MAKTKLSVMVVVLAVALVALVEGYGGRVGGRMEVKDVRRNEEVQRLGRFSVEEYNRRMGG 60
           MAKTKLSVMVVVLAVALVALVEGYGGRVGGRMEVKDVRRNEEVQRLGRFSVEEYNRRMGG
Sbjct: 1   MAKTKLSVMVVVLAVALVALVEGYGGRVGGRMEVKDVRRNEEVQRLGRFSVEEYNRRMGG 60

Query: 61  GGEVKFTAVVAAERQVVSGTKYYLRILGTQNGERKVFDSVVIVKPWIGSKRLLDFSPSAV 120
           GGEVKFTAVVAAERQVVSGTKYYLRILGTQNGERKVFDSVVIVKPWIGSKRLLDFSPSAV
Sbjct: 61  GGEVKFTAVVAAERQVVSGTKYYLRILGTQNGERKVFDSVVIVKPWIGSKRLLDFSPSAV 120

Query: 121 FRTPIFNF 129
           FRTPIFNF
Sbjct: 121 FRTPIFNF 128

BLAST of Csa6G022340 vs. TrEMBL
Match: A0A059VDR2_9ROSA (Cysteine proteinase inhibitor OS=Malus prunifolia PE=2 SV=1)

HSP 1 Score: 126.7 bits (317), Expect = 2.0e-26
Identity = 62/118 (52.54%), Postives = 87/118 (73.73%), Query Frame = 1

Query: 5   KLSVMVVVLAVALVALVEGYGGRVGGRMEVKDVRRNEEVQRLGRFSVEEYNRRMG----- 64
           K+ +  +++ +  VA   GYGG VGGR E+++V+ N+EVQ LGRFSVEEYNR+ G     
Sbjct: 3   KVPIFALLICLLFVAS-NGYGGMVGGRKEIENVKTNKEVQELGRFSVEEYNRQRGTQKMD 62

Query: 65  GGGEVKFTAVVAAERQVVSGTKYYLRILGTQNGERKVFDSVVIVKPWIGSKRLLDFSP 118
           GGGE++F  VV A+ QVVSG KYYL++   +NG  ++FDS V+VKPW+ SK+LL+F+P
Sbjct: 63  GGGELQFLEVVEAQSQVVSGIKYYLKVSAVRNGVHRLFDSEVVVKPWLRSKQLLNFAP 119

BLAST of Csa6G022340 vs. TrEMBL
Match: Q84XY6_MALDO (Cysteine proteinase inhibitor OS=Malus domestica PE=2 SV=1)

HSP 1 Score: 126.7 bits (317), Expect = 2.0e-26
Identity = 62/118 (52.54%), Postives = 87/118 (73.73%), Query Frame = 1

Query: 5   KLSVMVVVLAVALVALVEGYGGRVGGRMEVKDVRRNEEVQRLGRFSVEEYNRRMG----- 64
           K+ +  +++ +  VA   GYGG VGGR E+++V+ N+EVQ LGRFSVEEYNR+ G     
Sbjct: 3   KVPIFALLICLLFVAS-NGYGGMVGGRKEIENVKTNKEVQELGRFSVEEYNRQRGTQKMD 62

Query: 65  GGGEVKFTAVVAAERQVVSGTKYYLRILGTQNGERKVFDSVVIVKPWIGSKRLLDFSP 118
           GGGE++F  VV A+ QVVSG KYYL++   +NG  ++FDS V+VKPW+ SK+LL+F+P
Sbjct: 63  GGGELQFLEVVEAQSQVVSGIKYYLKVSAVRNGVHRLFDSEVVVKPWLRSKQLLNFAP 119

BLAST of Csa6G022340 vs. TrEMBL
Match: A0A058ZX43_EUCGR (Cysteine proteinase inhibitor OS=Eucalyptus grandis GN=EUGRSUZ_L00642 PE=3 SV=1)

HSP 1 Score: 123.6 bits (309), Expect = 1.7e-25
Identity = 69/124 (55.65%), Postives = 85/124 (68.55%), Query Frame = 1

Query: 9   MVVVLAVALVALVEG--YGGRVGGRMEVKDVRRNEEVQRLGRFSVEEYNR-----RMGGG 68
           ++ + A++L+A V G   G RVGGR  V DVR N+EVQ LGR+SVEEYNR     R GGG
Sbjct: 8   LITLTALSLLAAVSGGYVGPRVGGRTAVPDVRSNQEVQDLGRYSVEEYNRMQRWQRGGGG 67

Query: 69  G-------EVKFTAVVAAERQVVSGTKYYLRILGTQNGERKVFDSVVIVKPWIGSKRLLD 119
           G       E+ F  VV AERQVV+G KYYL+I G Q G RKVF+SVV+V+PW+ SK L+ 
Sbjct: 68  GRGGGDNGEIVFGEVVGAERQVVAGIKYYLKIEGMQRGARKVFESVVVVRPWVRSKELVT 127

BLAST of Csa6G022340 vs. TrEMBL
Match: A0A061EJ89_THECC (Cysteine proteinase inhibitor OS=Theobroma cacao GN=TCM_020180 PE=3 SV=1)

HSP 1 Score: 123.6 bits (309), Expect = 1.7e-25
Identity = 65/125 (52.00%), Postives = 89/125 (71.20%), Query Frame = 1

Query: 1   MAKTKLSVMVVVLAVALVAL--VEGYGGRVGGRMEVKDVRRNEEVQRLGRFSVEEYNRRM 60
           MAK ++ +++ +L+V  ++   V GYG  VGG+ E+ DV+ N+EVQ LGRFSVEE+NR  
Sbjct: 1   MAKAEVIILLSLLSVITLSFTAVNGYGTLVGGKTEINDVKTNKEVQELGRFSVEEFNRSR 60

Query: 61  GG-----GGEVKFTAVVAAERQVVSGTKYYLRILGTQNGERKVFDSVVIVKPWIGSKRLL 119
           GG     G E+ F+ VV A++QVVSG KYYL+I   Q+G  + F+SVV+VKPW  SK LL
Sbjct: 61  GGLRSNGGTELVFSQVVEAQKQVVSGIKYYLKIEAMQSGLTRTFESVVVVKPWEDSKELL 120

BLAST of Csa6G022340 vs. TAIR10
Match: AT2G31980.1 (AT2G31980.1 PHYTOCYSTATIN 2)

HSP 1 Score: 89.7 bits (221), Expect = 1.4e-18
Identity = 51/136 (37.50%), Postives = 83/136 (61.03%), Query Frame = 1

Query: 5   KLSVMVVVLAVALVALVEGYGGR-------VGGRMEVKDVRRNEEVQRLGRFSVEEYNRR 64
           K+S+++ +L   ++A+V             +GG+  V ++R N E+Q+LGR+ VE++N++
Sbjct: 6   KVSLVLSLLGFLVIAVVTPSAANPFRKSVVLGGKSGVPNIRTNREIQQLGRYCVEQFNQQ 65

Query: 65  ----MGGGGEV-----------KFTAVVAAERQVVSGTKYYLRILGTQ-NGERKVFDSVV 118
                G  G +           +F+ VV+A++QVV+G KYYLRI  TQ NG  ++FDSVV
Sbjct: 66  AQNEQGNIGSIAKTDTAISNPLQFSRVVSAQKQVVAGLKYYLRIEVTQPNGSTRMFDSVV 125

BLAST of Csa6G022340 vs. TAIR10
Match: AT5G47550.1 (AT5G47550.1 Cystatin/monellin superfamily protein)

HSP 1 Score: 67.8 bits (164), Expect = 5.7e-12
Identity = 41/119 (34.45%), Postives = 63/119 (52.94%), Query Frame = 1

Query: 1   MAKTKLSVMVVVLAVALVALVEGYGGRVGGRMEVKDVRRNEEVQRLGRFSVEEYNRRMGG 60
           M    + ++++ L V L+ L      RVGG   + +V  + +V  +G F+V EYN+R   
Sbjct: 1   MTSKVVFLLLLSLVVVLLPLYASAAARVGGWSPISNVT-DPQVVEIGEFAVSEYNKRSES 60

Query: 61  GGEVKFTAVVAAERQVVSGTKYYLRILGTQ-NGERKVFDSVVIVKPWIGSKRLLDFSPS 119
           G  +KF  VV+ E QVVSGT Y L++     +G  K + ++V  KPW+  + L  F P+
Sbjct: 61  G--LKFETVVSGETQVVSGTNYRLKVAANDGDGVSKNYLAIVWDKPWMKFRNLTSFEPA 116

BLAST of Csa6G022340 vs. TAIR10
Match: AT3G12490.2 (AT3G12490.2 cystatin B)

HSP 1 Score: 64.7 bits (156), Expect = 4.8e-11
Identity = 33/92 (35.87%), Postives = 58/92 (63.04%), Query Frame = 1

Query: 28  VGGRMEVKDVRRNEEVQRLGRFSVEEYNRRMGGGGEVKFTAVVAAERQVVSGTKYYLRIL 87
           VGG  +V   + + EV+ L RF+V+E+N++      ++F  VV A+ QVV+GT ++L + 
Sbjct: 37  VGGVGDVPANQNSGEVESLARFAVDEHNKKENA--LLEFARVVKAKEQVVAGTLHHLTLE 96

Query: 88  GTQNGERKVFDSVVIVKPWIGSKRLLDFSPSA 120
             + G++K++++ V VKPW+  K L +F P++
Sbjct: 97  ILEAGQKKLYEAKVWVKPWLNFKELQEFKPAS 126

BLAST of Csa6G022340 vs. TAIR10
Match: AT2G40880.1 (AT2G40880.1 cystatin A)

HSP 1 Score: 60.5 bits (145), Expect = 9.0e-10
Identity = 29/92 (31.52%), Postives = 55/92 (59.78%), Query Frame = 1

Query: 28  VGGRMEVKDVRRNEEVQRLGRFSVEEYNRRMGGGGEVKFTAVVAAERQVVSGTKYYLRIL 87
           +GG  +++  + + E++ L RF+++E+N++      ++F  +V A  QVV+GT Y+L + 
Sbjct: 35  LGGVHDLRGNQNSGEIESLARFAIQEHNKQQNK--ILEFKKIVKAREQVVAGTMYHLTLE 94

Query: 88  GTQNGERKVFDSVVIVKPWIGSKRLLDFSPSA 120
             +  + K F++ V VKPW+  K+L +F  S+
Sbjct: 95  AKEGDQTKNFEAKVWVKPWMNFKQLQEFKESS 124

BLAST of Csa6G022340 vs. TAIR10
Match: AT5G05110.1 (AT5G05110.1 Cystatin/monellin family protein)

HSP 1 Score: 53.9 bits (128), Expect = 8.4e-08
Identity = 38/134 (28.36%), Postives = 68/134 (50.75%), Query Frame = 1

Query: 1   MAKTKLSVMVVVLAVALVALVEGYGG------------------RVGGRMEVK-DVRRNE 60
           M   + S+ ++++ V+LV L+ G+G                   ++GG  + K D    +
Sbjct: 1   MDMRRASMCMMLICVSLV-LLSGFGQFVICSEEKGTYNDNVVKMKLGGFSDSKNDWNGGK 60

Query: 61  EVQRLGRFSVEEYNRRMGGGGEVKFTAVVAAERQVVSGTKYYLRILGTQNGERKVFDSVV 116
           E+  +  F+V+E+NRR      ++   V+ A  QVV+G  Y L +   + GE+K++++ V
Sbjct: 61  EIDDIALFAVQEHNRRENA--VLELARVLKATEQVVAGKLYRLTLEVIEAGEKKIYEAKV 120

BLAST of Csa6G022340 vs. NCBI nr
Match: gi|449441532|ref|XP_004138536.1| (PREDICTED: cysteine proteinase inhibitor B [Cucumis sativus])

HSP 1 Score: 250.4 bits (638), Expect = 1.7e-63
Identity = 128/128 (100.00%), Postives = 128/128 (100.00%), Query Frame = 1

Query: 1   MAKTKLSVMVVVLAVALVALVEGYGGRVGGRMEVKDVRRNEEVQRLGRFSVEEYNRRMGG 60
           MAKTKLSVMVVVLAVALVALVEGYGGRVGGRMEVKDVRRNEEVQRLGRFSVEEYNRRMGG
Sbjct: 1   MAKTKLSVMVVVLAVALVALVEGYGGRVGGRMEVKDVRRNEEVQRLGRFSVEEYNRRMGG 60

Query: 61  GGEVKFTAVVAAERQVVSGTKYYLRILGTQNGERKVFDSVVIVKPWIGSKRLLDFSPSAV 120
           GGEVKFTAVVAAERQVVSGTKYYLRILGTQNGERKVFDSVVIVKPWIGSKRLLDFSPSAV
Sbjct: 61  GGEVKFTAVVAAERQVVSGTKYYLRILGTQNGERKVFDSVVIVKPWIGSKRLLDFSPSAV 120

Query: 121 FRTPIFNF 129
           FRTPIFNF
Sbjct: 121 FRTPIFNF 128

BLAST of Csa6G022340 vs. NCBI nr
Match: gi|659131010|ref|XP_008465470.1| (PREDICTED: cysteine proteinase inhibitor B [Cucumis melo])

HSP 1 Score: 224.9 bits (572), Expect = 7.8e-56
Identity = 112/128 (87.50%), Postives = 119/128 (92.97%), Query Frame = 1

Query: 1   MAKTKLSVMVVVLAVALVALVEGYGGRVGGRMEVKDVRRNEEVQRLGRFSVEEYNRRMGG 60
           MAKTKLSVM+V  AVALV +VEGYGG+VGGRME+KDV+RNEEVQRLGRFSVEEYNRRMGG
Sbjct: 1   MAKTKLSVMLVAAAVALVVVVEGYGGKVGGRMEIKDVKRNEEVQRLGRFSVEEYNRRMGG 60

Query: 61  GGEVKFTAVVAAERQVVSGTKYYLRILGTQNGERKVFDSVVIVKPWIGSKRLLDFSPSAV 120
           GGEVKFTAVVAA+RQVVSGTKYYLRILG QNGERK+FDSVVIVKPWIGSKRLLDFSPS V
Sbjct: 61  GGEVKFTAVVAADRQVVSGTKYYLRILGIQNGERKIFDSVVIVKPWIGSKRLLDFSPSTV 120

Query: 121 FRTPIFNF 129
           F TP   F
Sbjct: 121 FSTPFSYF 128

BLAST of Csa6G022340 vs. NCBI nr
Match: gi|657953919|ref|XP_008365221.1| (PREDICTED: cysteine proteinase inhibitor B-like [Malus domestica])

HSP 1 Score: 126.7 bits (317), Expect = 2.9e-26
Identity = 62/118 (52.54%), Postives = 87/118 (73.73%), Query Frame = 1

Query: 5   KLSVMVVVLAVALVALVEGYGGRVGGRMEVKDVRRNEEVQRLGRFSVEEYNRRMG----- 64
           K+ +  +++ +  VA   GYGG VGGR E+++V+ N+EVQ LGRFSVEEYNR+ G     
Sbjct: 3   KVPIFALLICLLFVAS-NGYGGMVGGRKEIENVKTNKEVQELGRFSVEEYNRQRGTQKMD 62

Query: 65  GGGEVKFTAVVAAERQVVSGTKYYLRILGTQNGERKVFDSVVIVKPWIGSKRLLDFSP 118
           GGGE++F  VV A+ QVVSG KYYL++   +NG  ++FDS V+VKPW+ SK+LL+F+P
Sbjct: 63  GGGELQFLEVVEAQSQVVSGIKYYLKVSAVRNGVHRLFDSEVVVKPWLRSKQLLNFAP 119

BLAST of Csa6G022340 vs. NCBI nr
Match: gi|694396663|ref|XP_009373605.1| (PREDICTED: cysteine proteinase inhibitor B-like [Pyrus x bretschneideri])

HSP 1 Score: 126.3 bits (316), Expect = 3.8e-26
Identity = 62/118 (52.54%), Postives = 86/118 (72.88%), Query Frame = 1

Query: 5   KLSVMVVVLAVALVALVEGYGGRVGGRMEVKDVRRNEEVQRLGRFSVEEYNRRMG----- 64
           K+ +  +V+ +  VA   GYGG +GGR E+++V+ NEEVQ LGRFSVEEYNR+ G     
Sbjct: 3   KVPIFALVICLLFVAS-NGYGGMLGGRKEIENVKTNEEVQELGRFSVEEYNRQRGTQKMD 62

Query: 65  GGGEVKFTAVVAAERQVVSGTKYYLRILGTQNGERKVFDSVVIVKPWIGSKRLLDFSP 118
           GGGE++F  VV A+ QVVSG KYY+++   +NG   +FDS V+VKPW+ SK+LL+F+P
Sbjct: 63  GGGELQFLEVVEAQSQVVSGLKYYIKVSAVRNGVHMLFDSEVVVKPWLRSKQLLNFAP 119

BLAST of Csa6G022340 vs. NCBI nr
Match: gi|694391102|ref|XP_009371093.1| (PREDICTED: cysteine proteinase inhibitor B [Pyrus x bretschneideri])

HSP 1 Score: 125.6 bits (314), Expect = 6.5e-26
Identity = 62/118 (52.54%), Postives = 86/118 (72.88%), Query Frame = 1

Query: 5   KLSVMVVVLAVALVALVEGYGGRVGGRMEVKDVRRNEEVQRLGRFSVEEYNRRMG----- 64
           K+ +  +V+ +  VA   GYGG +GGR E+++V+ N+EVQ LGRFSVEEYNR+ G     
Sbjct: 3   KVPIFALVICLLFVAS-NGYGGMLGGRKEIENVKTNKEVQELGRFSVEEYNRQRGTQKMD 62

Query: 65  GGGEVKFTAVVAAERQVVSGTKYYLRILGTQNGERKVFDSVVIVKPWIGSKRLLDFSP 118
           GGGE++F  VV A+ QVVSG KYYL++   +NG   +FDS V+VKPW+ SK+LL+F+P
Sbjct: 63  GGGELQFLEVVEAQSQVVSGLKYYLKVSAVRNGVHMLFDSEVVVKPWLRSKQLLNFAP 119

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
CYT4_ORYSJ3.0e-2355.56Cysteine proteinase inhibitor 4 OS=Oryza sativa subsp. japonica GN=Os01g0915200 ... [more]
CYTB_HELAN6.9e-2051.55Cysteine proteinase inhibitor B OS=Helianthus annuus PE=1 SV=1[more]
CYT5_ORYSJ1.5e-1951.00Cysteine proteinase inhibitor 5 OS=Oryza sativa subsp. japonica GN=Os01g0915401 ... [more]
CYT2_ARATH2.5e-1737.50Cysteine proteinase inhibitor 2 OS=Arabidopsis thaliana GN=CYS2 PE=2 SV=2[more]
CYT10_ORYSJ5.5e-1743.64Cysteine proteinase inhibitor 10 OS=Oryza sativa subsp. japonica GN=Os04g0350100... [more]
Match NameE-valueIdentityDescription
A0A0A0KC20_CUCSA1.2e-63100.00Cysteine proteinase inhibitor OS=Cucumis sativus GN=Csa_6G022340 PE=3 SV=1[more]
A0A059VDR2_9ROSA2.0e-2652.54Cysteine proteinase inhibitor OS=Malus prunifolia PE=2 SV=1[more]
Q84XY6_MALDO2.0e-2652.54Cysteine proteinase inhibitor OS=Malus domestica PE=2 SV=1[more]
A0A058ZX43_EUCGR1.7e-2555.65Cysteine proteinase inhibitor OS=Eucalyptus grandis GN=EUGRSUZ_L00642 PE=3 SV=1[more]
A0A061EJ89_THECC1.7e-2552.00Cysteine proteinase inhibitor OS=Theobroma cacao GN=TCM_020180 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT2G31980.11.4e-1837.50 PHYTOCYSTATIN 2[more]
AT5G47550.15.7e-1234.45 Cystatin/monellin superfamily protein[more]
AT3G12490.24.8e-1135.87 cystatin B[more]
AT2G40880.19.0e-1031.52 cystatin A[more]
AT5G05110.18.4e-0828.36 Cystatin/monellin family protein[more]
Match NameE-valueIdentityDescription
gi|449441532|ref|XP_004138536.1|1.7e-63100.00PREDICTED: cysteine proteinase inhibitor B [Cucumis sativus][more]
gi|659131010|ref|XP_008465470.1|7.8e-5687.50PREDICTED: cysteine proteinase inhibitor B [Cucumis melo][more]
gi|657953919|ref|XP_008365221.1|2.9e-2652.54PREDICTED: cysteine proteinase inhibitor B-like [Malus domestica][more]
gi|694396663|ref|XP_009373605.1|3.8e-2652.54PREDICTED: cysteine proteinase inhibitor B-like [Pyrus x bretschneideri][more]
gi|694391102|ref|XP_009371093.1|6.5e-2652.54PREDICTED: cysteine proteinase inhibitor B [Pyrus x bretschneideri][more]
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR000010Cystatin_dom
IPR020381Proteinase inhibitor I25, cystatin, conserved region
IPR027214Cystatin
Vocabulary: Molecular Function
TermDefinition
GO:0004869cysteine-type endopeptidase inhibitor activity
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0010951 negative regulation of endopeptidase activity
biological_process GO:0044699 single-organism process
biological_process GO:0008150 biological_process
cellular_component GO:0005575 cellular_component
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0004869 cysteine-type endopeptidase inhibitor activity
This gene is associated with the following unigenes:
Unigene NameAnalysis NameSequence type in Unigene
CU095270cucumber EST collection version 3.0transcribed_cluster
CU147587cucumber EST collection version 3.0transcribed_cluster

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Csa6G022340.1Csa6G022340.1mRNA


The following transcribed_cluster feature(s) are associated with this gene:

Feature NameUnique NameType
CU095270CU095270transcribed_cluster
CU147587CU147587transcribed_cluster


Analysis Name: InterPro Annotations of cucumber (Chinese Long)
Date Performed: 2016-09-28
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000010Cystatin domainSMARTSM00043CY_4coord: 26..118
score: 8.
IPR020381Proteinase inhibitor I25, cystatin, conserved regionPRODOMPD001231coord: 42..117
score: 2.0
IPR020381Proteinase inhibitor I25, cystatin, conserved regionPFAMPF16845SQAPIcoord: 35..116
score: 2.9
IPR027214CystatinPANTHERPTHR11413CYSTATIN FAMILY MEMBERcoord: 6..119
score: 1.4
NoneNo IPR availableGENE3DG3DSA:3.10.450.10coord: 28..117
score: 8.3
NoneNo IPR availablePANTHERPTHR11413:SF38CYSTEINE PROTEINASE INHIBITOR 2coord: 6..119
score: 1.4
NoneNo IPR availableunknownSSF54403Cystatin/monellincoord: 34..117
score: 1.7