CSPI06G02870 (gene) Wild cucumber (PI 183967) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	CSPI06G02870
Type	gene
Organism	Cucumis sativus (Wild cucumber (PI 183967))
Description	Cysteine proteinase inhibitor
Location	Chr6 : 2312913 .. 2313517 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

TTTTCACTAATATAAACCCTCCCTCTCCTCTATTTCTACGAAGAGAATAAGAAATTAGAGAACGCTGGAATTAAATAATGGCGAAAACAAAATTGAGTGTCATGGTGGTAGTGCTGGCGGTGGCTTTGGTGGCTTTGGTGGCTTTGGTGGAAGGATATGGGGGGAGAGTAGGAGGAAGGATGGAGGTTAAAGACGTGAGGAGGAACGAGGAGGTGCAGCGATTAGGAAGATTCAGCGTGGAGGAGTACAATCGTAGGATGGGAGGCGGGGGAGAGGTGAAGTTCACGGCGGTAGTTGCGGCGGAGAGGCAAGTGGTGTCGGGAACGAAATACTATTTGAGAATTTTGGGGACTCAAAATGGGGAGAGGAAAGTTTTTGATTCGGTTGTGATTGTGAAGCCTTGGATTGGTTCTAAGCGCCTTCTCGACTTTTCTCCTTCCGCAGTTTTTAGAACTCCCATTTTTAATTTTTGATTTCTTTTTTTTTCTTATTTTCTTGGGAAAATGGAGTTTTCTTTTCTTTCTCAGAGTTGTGAAGCAGAAGAGGAGCTGTAAATTTTTCTTCTTTCTTCCCAGTGAGCAATAAGAAATAAGTATGAGTTTAAA

mRNA sequence

ATGGCGAAAACAAAATTGAGTGTCATGGTGGTAGTGCTGGCGGTGGCTTTGGTGGCTTTGGTGGCTTTGGTGGAAGGATATGGGGGGAGAGTAGGAGGAAGGATGGAGGTTAAAGACGTGAGGAGGAACGAGGAGGTGCAGCGATTAGGAAGATTCAGCGTGGAGGAGTACAATCGTAGGATGGGAGGCGGGGGAGAGGTGAAGTTCACGGCGGTAGTTGCGGCGGAGAGGCAAGTGGTGTCGGGAACGAAATACTATTTGAGAATTTTGGGGACTCAAAATGGGGAGAGGAAAGTTTTTGATTCGGTTGTGATTGTGAAGCCTTGGATTGGTTCTAAGCGCCTTCTCGACTTTTCTCCTTCCGCAGTTTTTAGAACTCCCATTTTTAATTTTTGA

Coding sequence (CDS)

ATGGCGAAAACAAAATTGAGTGTCATGGTGGTAGTGCTGGCGGTGGCTTTGGTGGCTTTGGTGGCTTTGGTGGAAGGATATGGGGGGAGAGTAGGAGGAAGGATGGAGGTTAAAGACGTGAGGAGGAACGAGGAGGTGCAGCGATTAGGAAGATTCAGCGTGGAGGAGTACAATCGTAGGATGGGAGGCGGGGGAGAGGTGAAGTTCACGGCGGTAGTTGCGGCGGAGAGGCAAGTGGTGTCGGGAACGAAATACTATTTGAGAATTTTGGGGACTCAAAATGGGGAGAGGAAAGTTTTTGATTCGGTTGTGATTGTGAAGCCTTGGATTGGTTCTAAGCGCCTTCTCGACTTTTCTCCTTCCGCAGTTTTTAGAACTCCCATTTTTAATTTTTGA

Homology

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BLAST of CSPI06G02870 vs. Swiss-Prot
Match: CYT4_ORYSJ (Cysteine proteinase inhibitor 4 OS=Oryza sativa subsp. japonica GN=Os01g0915200 PE=2 SV=1)

HSP 1 Score: 109.0 bits (271), Expect = 4.0e-23
Identity = 60/108 (55.56%), Postives = 74/108 (68.52%), Query Frame = 1


Query: 26  GYGGRVGGRMEVKDVRRNEEVQRLGRFSVEEYNRRMG----GGGEVKFTAVVAAERQVVS 85
           G G RVGGR EV+DV  + EVQ LGRFSVEE+NRR      G   ++F  VVAA+RQVVS
Sbjct: 45  GAGRRVGGRTEVRDVEGDREVQELGRFSVEEHNRRRRSRDCGDVRLEFGRVVAAQRQVVS 104

Query: 86  GTKYYLRI-------LGTQNGERKVFDSVVIVKPWIGSKRLLDFSPSA 123
           G KYYLR+        G   GE +VFD+VV+VKPW+ S+ LL F+P+A
Sbjct: 105 GLKYYLRVAAAEEGAAGQNGGEPRVFDAVVVVKPWLESRTLLTFAPAA 152

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BLAST of CSPI06G02870 vs. Swiss-Prot
Match: CYTB_HELAN (Cysteine proteinase inhibitor B OS=Helianthus annuus PE=1 SV=1)

HSP 1 Score: 97.8 bits (242), Expect = 9.3e-20
Identity = 50/97 (51.55%), Postives = 72/97 (74.23%), Query Frame = 1


Query: 32  GGRMEVKDVRRNEEVQRLGRFSVEEYNR----RMGGGGEVKFTAVVAAERQVVSGTKYYL 91
           GGR +VK+V+ + E+Q+LG +SV+EYNR    +  G G++KF+ V+AAE QVV+GTKYYL
Sbjct: 3   GGRTKVKNVKTDTEIQQLGSYSVDEYNRLQRTKKTGAGDLKFSQVIAAETQVVAGTKYYL 62

Query: 92  RILG-TQNGERKVFDSVVIVKPWIGSKRLLDFSPSAV 124
           +I   T+ G+ KVFD+ V+V+ W  SK+LL F P+ V
Sbjct: 63  KIEAITKGGKMKVFDAEVVVQSWKHSKKLLGFKPAPV 99

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BLAST of CSPI06G02870 vs. Swiss-Prot
Match: CYT5_ORYSJ (Cysteine proteinase inhibitor 5 OS=Oryza sativa subsp. japonica GN=Os01g0915401 PE=3 SV=2)

HSP 1 Score: 96.7 bits (239), Expect = 2.1e-19
Identity = 51/100 (51.00%), Postives = 67/100 (67.00%), Query Frame = 1


Query: 26  GYGGRVGGRMEVKDVRRNEEVQRLGRFSVEEYNRRMGG---GGEVKFTAVVAAERQVVSG 85
           G G +VGGR +V+DV  N EVQ LG F V E+NRR G    G  + F+ VVAA+ QVVSG
Sbjct: 44  GRGRKVGGRTDVEDVEGNREVQELGLFCVVEHNRRGGSATRGRGLVFSRVVAAQTQVVSG 103

Query: 86  TKYYLRILGTQNGERKVFDSVVIVKPWIGSKRLLDFSPSA 123
            KYYLRI   +  +  VFD+VV+VK W+ S+ ++ F P+A
Sbjct: 104 IKYYLRIAAQEADDELVFDAVVVVKAWVPSREMVSFVPAA 143

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BLAST of CSPI06G02870 vs. Swiss-Prot
Match: CYT10_ORYSJ (Cysteine proteinase inhibitor 10 OS=Oryza sativa subsp. japonica GN=Os04g0350100 PE=2 SV=1)

HSP 1 Score: 87.8 bits (216), Expect = 9.6e-17
Identity = 48/110 (43.64%), Postives = 68/110 (61.82%), Query Frame = 1


Query: 31  VGGRMEVKDVRRNEEVQRLGRFSVEEYNRRMGGGG----------EVKFTAVVAAERQVV 90
           VGGR E++DV  N+ VQ LGRF+V E+NRR+  GG          ++ F  VV A++QVV
Sbjct: 39  VGGRTEIRDVGSNKAVQSLGRFAVAEHNRRLRHGGSGGPADPVPVKLAFARVVEAQKQVV 98

Query: 91  SGTKYYLRIL--------GTQNGERKVFDSVVIVKPWIGSKRLLDFSPSA 123
           SG  YYL++         G   G  +VFD+VV+VK W+ SK L+ F+P++
Sbjct: 99  SGVAYYLKVAASARDPRGGAAAGGDRVFDAVVVVKAWLKSKELVSFTPAS 148

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BLAST of CSPI06G02870 vs. Swiss-Prot
Match: CYT10_ORYSI (Cysteine proteinase inhibitor 10 OS=Oryza sativa subsp. indica GN=OsI_014908 PE=3 SV=2)

HSP 1 Score: 87.8 bits (216), Expect = 9.6e-17
Identity = 48/110 (43.64%), Postives = 68/110 (61.82%), Query Frame = 1


Query: 31  VGGRMEVKDVRRNEEVQRLGRFSVEEYNRRMGGGG----------EVKFTAVVAAERQVV 90
           VGGR E++DV  N+ VQ LGRF+V E+NRR+  GG          ++ F  VV A++QVV
Sbjct: 39  VGGRTEIRDVGSNKAVQSLGRFAVAEHNRRLRHGGSGGPADPVPVKLAFARVVEAQKQVV 98

Query: 91  SGTKYYLRIL--------GTQNGERKVFDSVVIVKPWIGSKRLLDFSPSA 123
           SG  YYL++         G   G  +VFD+VV+VK W+ SK L+ F+P++
Sbjct: 99  SGVAYYLKVAASARDPRGGAAAGGDRVFDAVVVVKAWLKSKELVSFTPAS 148

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BLAST of CSPI06G02870 vs. TrEMBL
Match: A0A0A0KC20_CUCSA (Cysteine proteinase inhibitor OS=Cucumis sativus GN=Csa_6G022340 PE=3 SV=1)

HSP 1 Score: 239.2 bits (609), Expect = 2.9e-60
Identity = 128/131 (97.71%), Postives = 128/131 (97.71%), Query Frame = 1


Query: 1   MAKTKLSVMVVVLAVALVALVALVEGYGGRVGGRMEVKDVRRNEEVQRLGRFSVEEYNRR 60
           MAKTKLSVMVVVLAVAL   VALVEGYGGRVGGRMEVKDVRRNEEVQRLGRFSVEEYNRR
Sbjct: 1   MAKTKLSVMVVVLAVAL---VALVEGYGGRVGGRMEVKDVRRNEEVQRLGRFSVEEYNRR 60

Query: 61  MGGGGEVKFTAVVAAERQVVSGTKYYLRILGTQNGERKVFDSVVIVKPWIGSKRLLDFSP 120
           MGGGGEVKFTAVVAAERQVVSGTKYYLRILGTQNGERKVFDSVVIVKPWIGSKRLLDFSP
Sbjct: 61  MGGGGEVKFTAVVAAERQVVSGTKYYLRILGTQNGERKVFDSVVIVKPWIGSKRLLDFSP 120

Query: 121 SAVFRTPIFNF 132
           SAVFRTPIFNF
Sbjct: 121 SAVFRTPIFNF 128

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BLAST of CSPI06G02870 vs. TrEMBL
Match: A0A059VDR2_9ROSA (Cysteine proteinase inhibitor OS=Malus prunifolia PE=2 SV=1)

HSP 1 Score: 122.1 bits (305), Expect = 5.1e-25
Identity = 62/116 (53.45%), Postives = 84/116 (72.41%), Query Frame = 1


Query: 10  VVVLAVALVALVALVEGYGGRVGGRMEVKDVRRNEEVQRLGRFSVEEYNRRMG-----GG 69
           V + A+ +  L     GYGG VGGR E+++V+ N+EVQ LGRFSVEEYNR+ G     GG
Sbjct: 4   VPIFALLICLLFVASNGYGGMVGGRKEIENVKTNKEVQELGRFSVEEYNRQRGTQKMDGG 63

Query: 70  GEVKFTAVVAAERQVVSGTKYYLRILGTQNGERKVFDSVVIVKPWIGSKRLLDFSP 121
           GE++F  VV A+ QVVSG KYYL++   +NG  ++FDS V+VKPW+ SK+LL+F+P
Sbjct: 64  GELQFLEVVEAQSQVVSGIKYYLKVSAVRNGVHRLFDSEVVVKPWLRSKQLLNFAP 119

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BLAST of CSPI06G02870 vs. TrEMBL
Match: Q84XY6_MALDO (Cysteine proteinase inhibitor OS=Malus domestica PE=2 SV=1)

HSP 1 Score: 122.1 bits (305), Expect = 5.1e-25
Identity = 62/116 (53.45%), Postives = 84/116 (72.41%), Query Frame = 1


Query: 10  VVVLAVALVALVALVEGYGGRVGGRMEVKDVRRNEEVQRLGRFSVEEYNRRMG-----GG 69
           V + A+ +  L     GYGG VGGR E+++V+ N+EVQ LGRFSVEEYNR+ G     GG
Sbjct: 4   VPIFALLICLLFVASNGYGGMVGGRKEIENVKTNKEVQELGRFSVEEYNRQRGTQKMDGG 63

Query: 70  GEVKFTAVVAAERQVVSGTKYYLRILGTQNGERKVFDSVVIVKPWIGSKRLLDFSP 121
           GE++F  VV A+ QVVSG KYYL++   +NG  ++FDS V+VKPW+ SK+LL+F+P
Sbjct: 64  GELQFLEVVEAQSQVVSGIKYYLKVSAVRNGVHRLFDSEVVVKPWLRSKQLLNFAP 119

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BLAST of CSPI06G02870 vs. TrEMBL
Match: A0A058ZX43_EUCGR (Cysteine proteinase inhibitor OS=Eucalyptus grandis GN=EUGRSUZ_L00642 PE=3 SV=1)

HSP 1 Score: 120.2 bits (300), Expect = 1.9e-24
Identity = 72/128 (56.25%), Postives = 86/128 (67.19%), Query Frame = 1


Query: 7   SVMVVVLAVALVALVALVEGY-GGRVGGRMEVKDVRRNEEVQRLGRFSVEEYNR-----R 66
           SV+ ++   AL  L A+  GY G RVGGR  V DVR N+EVQ LGR+SVEEYNR     R
Sbjct: 4   SVLFLITLTALSLLAAVSGGYVGPRVGGRTAVPDVRSNQEVQDLGRYSVEEYNRMQRWQR 63

Query: 67  MGGGG-------EVKFTAVVAAERQVVSGTKYYLRILGTQNGERKVFDSVVIVKPWIGSK 122
            GGGG       E+ F  VV AERQVV+G KYYL+I G Q G RKVF+SVV+V+PW+ SK
Sbjct: 64  GGGGGRGGGDNGEIVFGEVVGAERQVVAGIKYYLKIEGMQRGARKVFESVVVVRPWVRSK 123

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BLAST of CSPI06G02870 vs. TrEMBL
Match: M5XGL1_PRUPE (Cysteine proteinase inhibitor OS=Prunus persica GN=PRUPE_ppa013391mg PE=3 SV=1)

HSP 1 Score: 119.8 bits (299), Expect = 2.5e-24
Identity = 63/118 (53.39%), Postives = 86/118 (72.88%), Query Frame = 1


Query: 10  VVVLAVALVALVALVEGYGG-RVGGRMEVKDVRRNEEVQRLGRFSVEEYNR------RMG 69
           V +LA+ +  L AL  GYGG +VGGR +++DV+ N+EVQ+LGRFSV EYNR      +  
Sbjct: 4   VPILALLICLLFALSNGYGGTKVGGRQQIEDVKTNKEVQKLGRFSVAEYNRTQRKSHQSN 63

Query: 70  GGGEVKFTAVVAAERQVVSGTKYYLRILGTQNGERKVFDSVVIVKPWIGSKRLLDFSP 121
           GGGE++F  VV A+ QVVSG KYYL++   +NG   +FDS V+VKPW+ SK+LL+F+P
Sbjct: 64  GGGELQFLEVVEAQSQVVSGIKYYLKVSAVRNGAHMLFDSEVVVKPWLHSKQLLNFAP 121

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BLAST of CSPI06G02870 vs. TAIR10
Match: AT2G31980.1 (AT2G31980.1 PHYTOCYSTATIN 2)

HSP 1 Score: 83.2 bits (204), Expect = 1.3e-16
Identity = 52/136 (38.24%), Postives = 86/136 (63.24%), Query Frame = 1


Query: 5   KLSVMVVVLAVALVALVALVEGYGGR----VGGRMEVKDVRRNEEVQRLGRFSVEEYNRR 64
           K+S+++ +L   ++A+V        R    +GG+  V ++R N E+Q+LGR+ VE++N++
Sbjct: 6   KVSLVLSLLGFLVIAVVTPSAANPFRKSVVLGGKSGVPNIRTNREIQQLGRYCVEQFNQQ 65

Query: 65  ----MGGGGEV-----------KFTAVVAAERQVVSGTKYYLRILGTQ-NGERKVFDSVV 121
                G  G +           +F+ VV+A++QVV+G KYYLRI  TQ NG  ++FDSVV
Sbjct: 66  AQNEQGNIGSIAKTDTAISNPLQFSRVVSAQKQVVAGLKYYLRIEVTQPNGSTRMFDSVV 125

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BLAST of CSPI06G02870 vs. TAIR10
Match: AT5G47550.1 (AT5G47550.1 Cystatin/monellin superfamily protein)

HSP 1 Score: 61.6 bits (148), Expect = 4.1e-10
Identity = 41/115 (35.65%), Postives = 64/115 (55.65%), Query Frame = 1


Query: 8   VMVVVLAVALVALVALVEGYGGRVGGRMEVKDVRRNEEVQRLGRFSVEEYNRRMGGGGEV 67
           V+ ++L   +V L+ L      RVGG   + +V  + +V  +G F+V EYN+R   G  +
Sbjct: 5   VVFLLLLSLVVVLLPLYASAAARVGGWSPISNVT-DPQVVEIGEFAVSEYNKRSESG--L 64

Query: 68  KFTAVVAAERQVVSGTKYYLRILGTQ-NGERKVFDSVVIVKPWIGSKRLLDFSPS 122
           KF  VV+ E QVVSGT Y L++     +G  K + ++V  KPW+  + L  F P+
Sbjct: 65  KFETVVSGETQVVSGTNYRLKVAANDGDGVSKNYLAIVWDKPWMKFRNLTSFEPA 116

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BLAST of CSPI06G02870 vs. TAIR10
Match: AT2G40880.1 (AT2G40880.1 cystatin A)

HSP 1 Score: 60.1 bits (144), Expect = 1.2e-09
Identity = 29/92 (31.52%), Postives = 57/92 (61.96%), Query Frame = 1


Query: 31  VGGRMEVKDVRRNEEVQRLGRFSVEEYNRRMGGGGEVKFTAVVAAERQVVSGTKYYLRIL 90
           +GG  +++  + + E++ L RF+++E+N++      ++F  +V A  QVV+GT Y+L + 
Sbjct: 35  LGGVHDLRGNQNSGEIESLARFAIQEHNKQQNK--ILEFKKIVKAREQVVAGTMYHLTLE 94

Query: 91  GTQNGERKVFDSVVIVKPWIGSKRLLDFSPSA 123
             +  + K F++ V VKPW+  K+L +F  S+
Sbjct: 95  AKEGDQTKNFEAKVWVKPWMNFKQLQEFKESS 124

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BLAST of CSPI06G02870 vs. TAIR10
Match: AT3G12490.2 (AT3G12490.2 cystatin B)

HSP 1 Score: 58.9 bits (141), Expect = 2.7e-09
Identity = 39/128 (30.47%), Postives = 77/128 (60.16%), Query Frame = 1


Query: 1   MAKTKLSVMVVVLAVALV--ALVALVEGYGGR----VGGRMEVKDVRRNEEVQRLGRFSV 60
           M +++  + +V  +++L   +L+A   G+       VGG  +V   + + EV+ L RF+V
Sbjct: 1   MMRSRFLLFIVFFSLSLFISSLIASDLGFCNEEMALVGGVGDVPANQNSGEVESLARFAV 60

Query: 61  EEYNRRMGGGGEVKFTAVVAAERQVVSGTKYYLRILGTQNGERKVFDSVVIVKPWIGSKR 120
           +E+N++      ++F  VV A+ QVV+GT ++L +   + G++K++++ V VKPW+  K 
Sbjct: 61  DEHNKKENA--LLEFARVVKAKEQVVAGTLHHLTLEILEAGQKKLYEAKVWVKPWLNFKE 120

Query: 121 LLDFSPSA 123
           L +F P++
Sbjct: 121 LQEFKPAS 126

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BLAST of CSPI06G02870 vs. TAIR10
Match: AT4G16500.1 (AT4G16500.1 Cystatin/monellin superfamily protein)

HSP 1 Score: 57.8 bits (138), Expect = 6.0e-09
Identity = 40/112 (35.71%), Postives = 68/112 (60.71%), Query Frame = 1


Query: 10  VVVLAVALVALVALVEGY--GGRVGGRMEVKDVRRNEEVQRLGRFSVEEYNRRMGGGGEV 69
           ++ L++ L+ LV++VEG   GG +G R  +K+V  + +V  + ++++EE+N+      ++
Sbjct: 6   LICLSLILLPLVSVVEGLGGGGGLGSRKPIKNV-SDPDVVAVAKYAIEEHNKE--SKEKL 65

Query: 70  KFTAVVAAERQVVSGTKYYLRILGTQNGER-KVFDSVVIVKPWIGSKRLLDF 119
            F  VV    QVVSGTKY L+I     G + K +++VV+ K W+ SK L  F
Sbjct: 66  VFVKVVEGTTQVVSGTKYDLKIAAKDGGGKIKNYEAVVVEKLWLHSKSLESF 114

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BLAST of CSPI06G02870 vs. NCBI nr
Match: gi|449441532|ref|XP_004138536.1| (PREDICTED: cysteine proteinase inhibitor B [Cucumis sativus])

HSP 1 Score: 239.2 bits (609), Expect = 4.1e-60
Identity = 128/131 (97.71%), Postives = 128/131 (97.71%), Query Frame = 1


Query: 1   MAKTKLSVMVVVLAVALVALVALVEGYGGRVGGRMEVKDVRRNEEVQRLGRFSVEEYNRR 60
           MAKTKLSVMVVVLAVAL   VALVEGYGGRVGGRMEVKDVRRNEEVQRLGRFSVEEYNRR
Sbjct: 1   MAKTKLSVMVVVLAVAL---VALVEGYGGRVGGRMEVKDVRRNEEVQRLGRFSVEEYNRR 60

Query: 61  MGGGGEVKFTAVVAAERQVVSGTKYYLRILGTQNGERKVFDSVVIVKPWIGSKRLLDFSP 120
           MGGGGEVKFTAVVAAERQVVSGTKYYLRILGTQNGERKVFDSVVIVKPWIGSKRLLDFSP
Sbjct: 61  MGGGGEVKFTAVVAAERQVVSGTKYYLRILGTQNGERKVFDSVVIVKPWIGSKRLLDFSP 120

Query: 121 SAVFRTPIFNF 132
           SAVFRTPIFNF
Sbjct: 121 SAVFRTPIFNF 128

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BLAST of CSPI06G02870 vs. NCBI nr
Match: gi|659131010|ref|XP_008465470.1| (PREDICTED: cysteine proteinase inhibitor B [Cucumis melo])

HSP 1 Score: 213.8 bits (543), Expect = 1.8e-52
Identity = 112/131 (85.50%), Postives = 119/131 (90.84%), Query Frame = 1


Query: 1   MAKTKLSVMVVVLAVALVALVALVEGYGGRVGGRMEVKDVRRNEEVQRLGRFSVEEYNRR 60
           MAKTKLSVM+V    A VALV +VEGYGG+VGGRME+KDV+RNEEVQRLGRFSVEEYNRR
Sbjct: 1   MAKTKLSVMLV---AAAVALVVVVEGYGGKVGGRMEIKDVKRNEEVQRLGRFSVEEYNRR 60

Query: 61  MGGGGEVKFTAVVAAERQVVSGTKYYLRILGTQNGERKVFDSVVIVKPWIGSKRLLDFSP 120
           MGGGGEVKFTAVVAA+RQVVSGTKYYLRILG QNGERK+FDSVVIVKPWIGSKRLLDFSP
Sbjct: 61  MGGGGEVKFTAVVAADRQVVSGTKYYLRILGIQNGERKIFDSVVIVKPWIGSKRLLDFSP 120

Query: 121 SAVFRTPIFNF 132
           S VF TP   F
Sbjct: 121 STVFSTPFSYF 128

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BLAST of CSPI06G02870 vs. NCBI nr
Match: gi|657953919|ref|XP_008365221.1| (PREDICTED: cysteine proteinase inhibitor B-like [Malus domestica])

HSP 1 Score: 122.1 bits (305), Expect = 7.3e-25
Identity = 62/116 (53.45%), Postives = 84/116 (72.41%), Query Frame = 1


Query: 10  VVVLAVALVALVALVEGYGGRVGGRMEVKDVRRNEEVQRLGRFSVEEYNRRMG-----GG 69
           V + A+ +  L     GYGG VGGR E+++V+ N+EVQ LGRFSVEEYNR+ G     GG
Sbjct: 4   VPIFALLICLLFVASNGYGGMVGGRKEIENVKTNKEVQELGRFSVEEYNRQRGTQKMDGG 63

Query: 70  GEVKFTAVVAAERQVVSGTKYYLRILGTQNGERKVFDSVVIVKPWIGSKRLLDFSP 121
           GE++F  VV A+ QVVSG KYYL++   +NG  ++FDS V+VKPW+ SK+LL+F+P
Sbjct: 64  GELQFLEVVEAQSQVVSGIKYYLKVSAVRNGVHRLFDSEVVVKPWLRSKQLLNFAP 119

[Close]

BLAST of CSPI06G02870 vs. NCBI nr
Match: gi|694406755|ref|XP_009378164.1| (PREDICTED: cysteine proteinase inhibitor B-like [Pyrus x bretschneideri])

HSP 1 Score: 120.9 bits (302), Expect = 1.6e-24
Identity = 62/119 (52.10%), Postives = 84/119 (70.59%), Query Frame = 1


Query: 10  VVVLAVALVALVALVEGYGGRVGGRMEVKDVRRNEEVQRLGRFSVEEYN------RRMGG 69
           V + A+ +  L  L  GYGG VGGR ++++V+ N+EVQ LGRFSV EYN      R+M G
Sbjct: 4   VPIFALLICLLFVLSNGYGGMVGGRRQIENVKSNKEVQELGRFSVAEYNSRQKASRKMDG 63

Query: 70  GGEVKFTAVVAAERQVVSGTKYYLRILGTQNGERKVFDSVVIVKPWIGSKRLLDFSPSA 123
           GGE++F  VV A+ QVVSG KYYL++   +NG   +FDS V+VKPW+ SK+LL+F+P A
Sbjct: 64  GGELQFLEVVEAQSQVVSGIKYYLKVSAVKNGAHMLFDSEVVVKPWLRSKQLLNFAPHA 122

[Close]

BLAST of CSPI06G02870 vs. NCBI nr
Match: gi|694396663|ref|XP_009373605.1| (PREDICTED: cysteine proteinase inhibitor B-like [Pyrus x bretschneideri])

HSP 1 Score: 120.9 bits (302), Expect = 1.6e-24
Identity = 61/116 (52.59%), Postives = 83/116 (71.55%), Query Frame = 1


Query: 10  VVVLAVALVALVALVEGYGGRVGGRMEVKDVRRNEEVQRLGRFSVEEYNRRMG-----GG 69
           V + A+ +  L     GYGG +GGR E+++V+ NEEVQ LGRFSVEEYNR+ G     GG
Sbjct: 4   VPIFALVICLLFVASNGYGGMLGGRKEIENVKTNEEVQELGRFSVEEYNRQRGTQKMDGG 63

Query: 70  GEVKFTAVVAAERQVVSGTKYYLRILGTQNGERKVFDSVVIVKPWIGSKRLLDFSP 121
           GE++F  VV A+ QVVSG KYY+++   +NG   +FDS V+VKPW+ SK+LL+F+P
Sbjct: 64  GELQFLEVVEAQSQVVSGLKYYIKVSAVRNGVHMLFDSEVVVKPWLRSKQLLNFAP 119

The following BLAST results are available for this feature:

BLAST of CSPI06G02870 vs. Swiss-Prot


Match Name	E-value	Identity	Description
CYT4_ORYSJ	4.0e-23	55.56	Cysteine proteinase inhibitor 4 OS=Oryza sativa subsp. japonica GN=Os01g0915200 ...	[more]
CYTB_HELAN	9.3e-20	51.55	Cysteine proteinase inhibitor B OS=Helianthus annuus PE=1 SV=1	[more]
CYT5_ORYSJ	2.1e-19	51.00	Cysteine proteinase inhibitor 5 OS=Oryza sativa subsp. japonica GN=Os01g0915401 ...	[more]
CYT10_ORYSJ	9.6e-17	43.64	Cysteine proteinase inhibitor 10 OS=Oryza sativa subsp. japonica GN=Os04g0350100...	[more]
CYT10_ORYSI	9.6e-17	43.64	Cysteine proteinase inhibitor 10 OS=Oryza sativa subsp. indica GN=OsI_014908 PE=...	[more]

BLAST of CSPI06G02870 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KC20_CUCSA	2.9e-60	97.71	Cysteine proteinase inhibitor OS=Cucumis sativus GN=Csa_6G022340 PE=3 SV=1	[more]
A0A059VDR2_9ROSA	5.1e-25	53.45	Cysteine proteinase inhibitor OS=Malus prunifolia PE=2 SV=1	[more]
Q84XY6_MALDO	5.1e-25	53.45	Cysteine proteinase inhibitor OS=Malus domestica PE=2 SV=1	[more]
A0A058ZX43_EUCGR	1.9e-24	56.25	Cysteine proteinase inhibitor OS=Eucalyptus grandis GN=EUGRSUZ_L00642 PE=3 SV=1	[more]
M5XGL1_PRUPE	2.5e-24	53.39	Cysteine proteinase inhibitor OS=Prunus persica GN=PRUPE_ppa013391mg PE=3 SV=1	[more]

BLAST of CSPI06G02870 vs. TAIR10


Match Name	E-value	Identity	Description
AT2G31980.1	1.3e-16	38.24	PHYTOCYSTATIN 2	[more]
AT5G47550.1	4.1e-10	35.65	Cystatin/monellin superfamily protein	[more]
AT2G40880.1	1.2e-09	31.52	cystatin A	[more]
AT3G12490.2	2.7e-09	30.47	cystatin B	[more]
AT4G16500.1	6.0e-09	35.71	Cystatin/monellin superfamily protein	[more]

BLAST of CSPI06G02870 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|449441532\|ref\|XP_004138536.1\|	4.1e-60	97.71	PREDICTED: cysteine proteinase inhibitor B [Cucumis sativus]	[more]
gi\|659131010\|ref\|XP_008465470.1\|	1.8e-52	85.50	PREDICTED: cysteine proteinase inhibitor B [Cucumis melo]	[more]
gi\|657953919\|ref\|XP_008365221.1\|	7.3e-25	53.45	PREDICTED: cysteine proteinase inhibitor B-like [Malus domestica]	[more]
gi\|694406755\|ref\|XP_009378164.1\|	1.6e-24	52.10	PREDICTED: cysteine proteinase inhibitor B-like [Pyrus x bretschneideri]	[more]
gi\|694396663\|ref\|XP_009373605.1\|	1.6e-24	52.59	PREDICTED: cysteine proteinase inhibitor B-like [Pyrus x bretschneideri]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR000010	Cystatin_dom
IPR020381	Proteinase inhibitor I25, cystatin, conserved region
IPR027214	Cystatin

Vocabulary: **Molecular Function**
Term	Definition
GO:0004869	cysteine-type endopeptidase inhibitor activity

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0010951	negative regulation of endopeptidase activity
biological_process	GO:0044699	single-organism process
biological_process	GO:0008150	biological_process
cellular_component	GO:0005575	cellular_component
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0004869	cysteine-type endopeptidase inhibitor activity

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI06G02870.1	CSPI06G02870.1	mRNA

InterPro

Analysis Name: InterPro Annotations of cucumber (PI183967)
Date Performed: 2017-01-17


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000010	Cystatin domain	SMART	SM00043	CY_4	coord: 29..121 score: 8.
IPR020381	Proteinase inhibitor I25, cystatin, conserved region	PRODOM	PD001231		coord: 45..120 score: 2.0
IPR020381	Proteinase inhibitor I25, cystatin, conserved region	PFAM	PF16845	SQAPI	coord: 38..119 score: 3.2
IPR027214	Cystatin	PANTHER	PTHR11413	CYSTATIN FAMILY MEMBER	coord: 1..122 score: 5.4
None	No IPR available	GENE3D	G3DSA:3.10.450.10		coord: 31..120 score: 8.9
None	No IPR available	PANTHER	PTHR11413:SF38	CYSTEINE PROTEINASE INHIBITOR 2	coord: 1..122 score: 5.4
None	No IPR available	unknown	SSF54403	Cystatin/monellin	coord: 37..120 score: 1.83

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
CSPI06G02870	Csa6G022340	Cucumber (Chinese Long) v2	cpicuB314
CSPI06G02870	MELO3C027001	Melon (DHL92) v3.5.1	cpimeB468
CSPI06G02870	ClCG08G003930	Watermelon (Charleston Gray)	cpiwcgB530
CSPI06G02870	Lsi08G003220	Bottle gourd (USVL1VR-Ls)	cpilsiB483
CSPI06G02870	CsaV3_6G003120	Cucumber (Chinese Long) v3	cpicucB364
CSPI06G02870	Cla97C08G147270	Watermelon (97103) v2	cpiwmbB516
CSPI06G02870	Bhi04G001668	Wax gourd	cpiwgoB586
CSPI06G02870	Cucsa.365610	Cucumber (Gy14) v1	cgycpiB540
CSPI06G02870	CmaCh08G000940	Cucurbita maxima (Rimu)	cmacpiB931
CSPI06G02870	CmoCh08G000980	Cucurbita moschata (Rifu)	cmocpiB910
CSPI06G02870	Cp4.1LG17g09930	Cucurbita pepo (Zucchini)	cpecpiB336
CSPI06G02870	CsGy6G002950	Cucumber (Gy14) v2	cgybcpiB288
CSPI06G02870	Carg04920	Silver-seed gourd	carcpiB0098

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
CSPI06G02870	Cucurbita maxima (Rimu)	cmacpiB381
CSPI06G02870	Cucurbita moschata (Rifu)	cmocpiB370
CSPI06G02870	Watermelon (97103) v1	cpiwmB491
CSPI06G02870	Cucurbita pepo (Zucchini)	cpecpiB150
CSPI06G02870	Melon (DHL92) v3.6.1	cpimedB464
CSPI06G02870	Silver-seed gourd	carcpiB0254