Csa4G288110 (gene) Cucumber (Chinese Long) v2 | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Csa4G288110
Type	gene
Organism	Cucumis. sativus (Cucumber (Chinese Long) v2)
Description	Lipoxygenase; contains IPR000907 (Lipoxygenase), IPR027433 (Lipoxygenase, domain 3)
Location	Chr4 : 11202996 .. 11205660 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTTAACTGTAGATTCATTATCAGAAGTCAGAAGTATGGAGTTTTATGTGCCACGAGATGAAGCGTTCTCCATATCAAAACAGGGAAATACATTGATGAAGGCATTTAATTCCATGTTACATGGCTTCTTATTACCAGCTTTAGAGGACAACTTCAATAAACATTATTCCACTCTCTTTGAAACAAAACATGATCGTTCTCCTTGTCCTAAACTTGTTCAAATCCTTACTGATAAGGCCATGGATATCTTACTCTTGCATTCTTCGCAGACATCCTATGGTAAACATAACAACTTTATAATAATATATTTTGTCTTGTTTTATTTTATTTATTATATTGAGATGGAGTTTAATTTAATTTCTTCATTATTTTTCAGGGGACAAATTCTTTTGGTTTAGAGATGAGGAATTTGCTAGACAGACCCTTGCAGGTCTCAATCCGTATAGCATAAGACTTGTCACGGTACGACATGCAAGCTTGAATCATTTTACTTCCATAGTAAATAAAAATAAAAATTATCCAAGAAAGATACTTGGGTGGTTTCTTTTGTTTCTGTGGATGATAAAAGTAGGATATATAAAGATTAGTGCATCTTTGAGACGCACCTATAAAGTACTTTTTGATCTATGAATCATCTCTTTTGTAATCTCTTTTCTTGTCTATGAATCATCAGCACTTTAGGCCTTGGGGTGTGGTGAGGGTGTAAGGTGTAAGGTGATGTCAACCTAAGTTGAAATGTACGTCTTGATGCACTTTCGGATCTCTCTTACTTGCTGTGAATATTTTTTAAAAGAAAAAAAAAGAAAAAAAAAAGACATACCCAAGTATTGCTCAAGAATCATTATTGTATCTACCTGTGCAACACAAACTGCACATTAAAAGAGTTGTGAAGGTTTTTCTCAAAAAGAAGAGTTGCACAAGTTGTATCTTAAGTTTTCTTTCTTAAATTTTACAGTAACACACTTGTACCTCACAACATTTATAAGATATATACCAACAAATGATTAATATTCTTTATCGATTGATTTTGAGATGAAACTTTATAGTTTAATATTCAACAAAAATAAAAGTAAATATTATTAGTGAACCTAAAGAAACACTATCGATAATTGATATAACTTTGGAGTATAAATGAAAAGACTAATTATGCTAAAACAAAAATTTATCCAGGAATGGCCATTGAAGAGTAAGCTTGACCCTTCTATTTATGGTTCCCCTGAATCCGCAATCACTGATGAAATAGTTGAGCAACAAATAAAAGGAGTCATGAGTCTTGATGAGGTAATATATATTATATCAATATTAATATATATGGTTTCACTTAGATTATATTAATGATATAATTGTAGCAATTAATATTTGAATATGTATCATTTGTTTGTGAGAATGCAGGCATTGAAACAAAAGAAGTTATTTATATTAGATTATCATGATGTGTTCATACCATATGTAGCAAAGGTAAGAAAGCTGAAAGGGAGAACATTGTACGGTTCAAGAACATTATTTTTCTTAAACCCAGATAGCACTTTAAGGCCACTAGCTATTGAGCTGAGCAGACCACCCATTGATAACGAACCACAATGGAAAGATGTTTTCACACCATGTTGGGATGCCTATGGCCTTTGGCTTTGGAGGATCGCCAAAGCTCATGTTCTCGCTCATGACTCTGGTCATCACCAACTTATTACTCATTGGTCTGTATTAATTGATATGTTATTAAATTTTACTTGTACCAATTCAGCTGAAGTTTTTGGTTTTAATTGGTGATTTAAGAGTATATTTGTTGCAGGCTGAGAACTCATTGCTGCGTGGAACCTTATGTGATTGCTACACACAGACAACTAAGTGCAATGCATCCTATGTATAGACTATTGCATCCGCACCTTCGGTATACCATGGCGATCAATGCAGCGGCTAGGGAAGCCCTTATCAGTGTAGAAGGGCTTATTGAGAATATCTTCTCAACAGGAAAATATTCTATGGAAATTAGTTCCTTGGTCTATGACAAACAATGGCAATTCAACTTAGAAGCTTTACCTGCTGACTTAATTCACAGGTAATTTTATCTTTAAGACTGAAAAAACTAATGCAAAGTAACCTAACTCGATAAATATATTTTCATGCCTTAATCTCATACAAATATCAATTAAAACAAGGATAATTGTTTTAAGATATTCAAGAAGACTTAAAATACTAATGCATTTTTTATTTTCACTCTACTAAATTTTTATTTGTTTTTTTATTCTCTTTACCAATGTTTTCAAAGAGCAGAAATAATATTTTAAGAACTAAATTAACCATATTTAGTAGAGTGCATTTTTTTAAAAGAATATATAAGTTAATAGATGCATTCGAACATCTTTGCCAGGTAGACATAGCACCATCCTTATTTTTGTTTCATTAATATCAAAAAATAAGGACATGAACCAAGGAATGTCCAAAGAAAAGGACTAGAGAGTATAGCCCAACAAACCCAAATAATAGTTATTACAAAAGCATGAATGATTAATGCAATAGAACCTAGCCTACAAAAGTTTAATACAATAGAACTCGCCTATAAAATTAGCTCTACTACTCTAGAGGCCCGTGAGTACAAATTACCAATCGTTAACAAATGATCATATATGTTCTGTGCTTGTTCTTTAAAAGGGGATTAGCTGTTGA

mRNA sequence

ATGTTAACTGTAGATTCATTATCAGAAGTCAGAAGTATGGAGTTTTATGTGCCACGAGATGAAGCGTTCTCCATATCAAAACAGGGAAATACATTGATGAAGGCATTTAATTCCATGTTACATGGCTTCTTATTACCAGCTTTAGAGGACAACTTCAATAAACATTATTCCACTCTCTTTGAAACAAAACATGATCGTTCTCCTTGTCCTAAACTTGTTCAAATCCTTACTGATAAGGCCATGGATATCTTACTCTTGCATTCTTCGCAGACATCCTATGGGGACAAATTCTTTTGGTTTAGAGATGAGGAATTTGCTAGACAGACCCTTGCAGGTCTCAATCCGTATAGCATAAGACTTGTCACGGAATGGCCATTGAAGAGTAAGCTTGACCCTTCTATTTATGGTTCCCCTGAATCCGCAATCACTGATGAAATAGTTGAGCAACAAATAAAAGGAGTCATGAGTCTTGATGAGGGGATTAGCTGTTGA

Coding sequence (CDS)

ATGTTAACTGTAGATTCATTATCAGAAGTCAGAAGTATGGAGTTTTATGTGCCACGAGATGAAGCGTTCTCCATATCAAAACAGGGAAATACATTGATGAAGGCATTTAATTCCATGTTACATGGCTTCTTATTACCAGCTTTAGAGGACAACTTCAATAAACATTATTCCACTCTCTTTGAAACAAAACATGATCGTTCTCCTTGTCCTAAACTTGTTCAAATCCTTACTGATAAGGCCATGGATATCTTACTCTTGCATTCTTCGCAGACATCCTATGGGGACAAATTCTTTTGGTTTAGAGATGAGGAATTTGCTAGACAGACCCTTGCAGGTCTCAATCCGTATAGCATAAGACTTGTCACGGAATGGCCATTGAAGAGTAAGCTTGACCCTTCTATTTATGGTTCCCCTGAATCCGCAATCACTGATGAAATAGTTGAGCAACAAATAAAAGGAGTCATGAGTCTTGATGAGGGGATTAGCTGTTGA

Protein sequence

MLTVDSLSEVRSMEFYVPRDEAFSISKQGNTLMKAFNSMLHGFLLPALEDNFNKHYSTLFETKHDRSPCPKLVQILTDKAMDILLLHSSQTSYGDKFFWFRDEEFARQTLAGLNPYSIRLVTEWPLKSKLDPSIYGSPESAITDEIVEQQIKGVMSLDEGISC*

Homology

[Close]

BLAST of Csa4G288110 vs. Swiss-Prot
Match: LOX21_SOLTU (Linoleate 13S-lipoxygenase 2-1, chloroplastic OS=Solanum tuberosum GN=LOX2.1 PE=1 SV=1)

HSP 1 Score: 138.7 bits (348), Expect = 5.9e-32
Identity = 83/181 (45.86%), Postives = 107/181 (59.12%), Query Frame = 1


Query: 5   DSLSEVRSMEFYVPRDEAFS----ISKQGNTLMKAFNSMLHGFLLPALEDNFNK------ 64
           D LSE RS   YVPRDEAFS    ++  GNT+     S+LH  ++PALE           
Sbjct: 283 DPLSETRSTFVYVPRDEAFSEVKSVAFSGNTVY----SVLHA-VVPALESVVTDPNLGFP 342

Query: 65  HY---STLFETKHDR-----------SPCPKLVQILTDKAMDILLLHSSQTSYGDKFFWF 124
           H+    +LF    D            +  P+L++ ++D   D+LL  S Q    DKF WF
Sbjct: 343 HFPAIDSLFNVGVDLPGLGDKKSGLFNVVPRLIKAISDTRKDVLLFESPQLVQRDKFSWF 402

Query: 125 RDEEFARQTLAGLNPYSIRLVTEWPLKSKLDPSIYGSPESAITDEIVEQQIKGVMSLDEG 162
           RD EFARQTLAGLNPYSIRLVTEWPL+SKLDP +YG PES IT E++E++I   M++++ 
Sbjct: 403 RDVEFARQTLAGLNPYSIRLVTEWPLRSKLDPKVYGPPESEITKELIEKEIGNYMTVEQA 458

[Close]

BLAST of Csa4G288110 vs. Swiss-Prot
Match: LOXC2_ORYSJ (Probable lipoxygenase 8, chloroplastic OS=Oryza sativa subsp. japonica GN=CM-LOX2 PE=2 SV=1)

HSP 1 Score: 126.3 bits (316), Expect = 3.0e-28
Identity = 78/180 (43.33%), Postives = 102/180 (56.67%), Query Frame = 1


Query: 5   DSLSEVRSMEFYVPRDEAFSISKQGNTLMKAFNSMLHGFL---LPALEDNFNKH------ 64
           D  SE R    YVPRDE FS  K    L+K   S+LH  +     AL DN + +      
Sbjct: 322 DPKSETRKGNVYVPRDEEFSEVKNAQFLLKTLQSVLHAAVPAAQSALIDNLSLNLPFPSF 381

Query: 65  --YSTLFETKHD----------RSPCPKLVQILTDKAMD-ILLLHSSQTSYGDKFFWFRD 124
                LFE   +           S  P+L+++L D   D ILL  +      DKF W RD
Sbjct: 382 FVIDKLFEDGVELPGVEKLGFLHSIVPRLLELLRDSPGDKILLFDTPANVQKDKFAWLRD 441

Query: 125 EEFARQTLAGLNPYSIRLVTEWPLKSKLDPSIYGSPESAITDEIVEQQIKGVMSLDEGIS 163
           EEFAR+TLAG+NPY+I LV E+PLKSKLDP++YG  ESAIT +++E+Q++ VM+++E IS
Sbjct: 442 EEFARETLAGINPYAIELVREFPLKSKLDPAVYGPAESAITADLLEEQMRRVMTVEEAIS 501

[Close]

BLAST of Csa4G288110 vs. Swiss-Prot
Match: LOX2_ARATH (Lipoxygenase 2, chloroplastic OS=Arabidopsis thaliana GN=LOX2 PE=1 SV=1)

HSP 1 Score: 116.7 bits (291), Expect = 2.4e-25
Identity = 72/163 (44.17%), Postives = 93/163 (57.06%), Query Frame = 1


Query: 14  EFYVPRDEAFSISK----QGNTLMKAFNSM---LHGFLLPALEDNFN-KHYSTLFETKHD 73
           EFYVPRDE FS +K     G  ++ A  S+   +   LL   E   + K    LFE    
Sbjct: 294 EFYVPRDEEFSTAKGTSFTGKAVLAALPSIFPQIESVLLSPQEPFPHFKAIQNLFEEGIQ 353

Query: 74  RSP-------CPKLVQILTDKAMDILLLHSSQTSYGDKFFWFRDEEFARQTLAGLNPYSI 133
                      P++++ L +   DIL   +      D+F W RD+EFARQTLAGLNPYSI
Sbjct: 354 LPKDAGLLPLLPRIIKALGEAQDDILQFDAPVLINRDRFSWLRDDEFARQTLAGLNPYSI 413

Query: 134 RLVTEWPLKSKLDPSIYGSPESAITDEIVEQQIKGVMSLDEGI 162
           +LV EWPL SKLDP++YG P S IT EIVE+++KG M++DE +
Sbjct: 414 QLVEEWPLISKLDPAVYGDPTSLITWEIVEREVKGNMTVDEAL 456

[Close]

BLAST of Csa4G288110 vs. Swiss-Prot
Match: LOXC1_ORYSJ (Lipoxygenase 7, chloroplastic OS=Oryza sativa subsp. japonica GN=CM-LOX1 PE=2 SV=2)

HSP 1 Score: 111.3 bits (277), Expect = 1.0e-23
Identity = 76/180 (42.22%), Postives = 96/180 (53.33%), Query Frame = 1


Query: 5   DSLSEVRSMEFYVPRDEAFSISKQGNTLMKAFNSML-------HGFLLPALEDN--FNKH 64
           D  SE R    YVPRDE FS  K+   L K   S+L          LL  L+ N  F   
Sbjct: 305 DPKSETRKGNVYVPRDEEFSPEKEDYFLRKTVGSVLQAAVPAAQSLLLDKLKWNLPFPSF 364

Query: 65  Y--STLFETKHDRSPCPKL------VQILTDKAMD-----ILLLHSSQTSYGDKFFWFRD 124
           +    LFE   +     KL      V  L +   D     IL   +      DKF W RD
Sbjct: 365 FVIDKLFEDGVELPGVDKLNFLESVVPRLLEHLRDTPAEKILRFETPANIQKDKFAWLRD 424

Query: 125 EEFARQTLAGLNPYSIRLVTEWPLKSKLDPSIYGSPESAITDEIVEQQIKGVMSLDEGIS 163
           EEFAR+TLAG+NPY+I LV E+PLKSKLDP++YG  ESAIT +++E+Q++ VM+++E IS
Sbjct: 425 EEFARETLAGINPYAIELVREFPLKSKLDPAVYGPAESAITADLLEEQMRRVMTVEEAIS 484

[Close]

BLAST of Csa4G288110 vs. Swiss-Prot
Match: LOX23_HORVU (Lipoxygenase 2.3, chloroplastic OS=Hordeum vulgare GN=LOX2.3 PE=1 SV=1)

HSP 1 Score: 110.9 bits (276), Expect = 1.3e-23
Identity = 68/179 (37.99%), Postives = 92/179 (51.40%), Query Frame = 1


Query: 5   DSLSEVRSMEFYVPRDEAFSISKQGNTLMKAFNSMLHGFLLPALEDNFNK-----HYSTL 64
           D  +E RS   YVPRDE FS  K          S LH  +LPA+    N      H+  +
Sbjct: 281 DPETETRSSPVYVPRDEQFSDVKGRTFSATTLRSGLHA-ILPAVAPLLNNSHGFSHFPAI 340

Query: 65  FETKHDRSPCP-----------------KLVQILTDKAMDILLLHSSQTSYGDKFFWFRD 124
                D  P P                 ++VQ++ D    +L     +    D+F WFRD
Sbjct: 341 DALYSDGIPLPVDGHGGNSFNVINDVIPRVVQMIEDTTEHVLRFEVPEMLERDRFSWFRD 400

Query: 125 EEFARQTLAGLNPYSIRLVTEWPLKSKLDPSIYGSPESAITDEIVEQQIKGVMSLDEGI 162
           EEFARQTLAGLNP  IR +TE+P+ SKLDP++YG  ESA++ EI+E+ + G M+++E +
Sbjct: 401 EEFARQTLAGLNPICIRRLTEFPIVSKLDPAVYGPAESALSKEILEKMMNGRMTVEEAM 458

[Close]

BLAST of Csa4G288110 vs. TrEMBL
Match: A0A0A0KWY8_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_4G288110 PE=4 SV=1)

HSP 1 Score: 330.5 bits (846), Expect = 1.2e-87
Identity = 163/163 (100.00%), Postives = 163/163 (100.00%), Query Frame = 1


Query: 1   MLTVDSLSEVRSMEFYVPRDEAFSISKQGNTLMKAFNSMLHGFLLPALEDNFNKHYSTLF 60
           MLTVDSLSEVRSMEFYVPRDEAFSISKQGNTLMKAFNSMLHGFLLPALEDNFNKHYSTLF
Sbjct: 1   MLTVDSLSEVRSMEFYVPRDEAFSISKQGNTLMKAFNSMLHGFLLPALEDNFNKHYSTLF 60

Query: 61  ETKHDRSPCPKLVQILTDKAMDILLLHSSQTSYGDKFFWFRDEEFARQTLAGLNPYSIRL 120
           ETKHDRSPCPKLVQILTDKAMDILLLHSSQTSYGDKFFWFRDEEFARQTLAGLNPYSIRL
Sbjct: 61  ETKHDRSPCPKLVQILTDKAMDILLLHSSQTSYGDKFFWFRDEEFARQTLAGLNPYSIRL 120

Query: 121 VTEWPLKSKLDPSIYGSPESAITDEIVEQQIKGVMSLDEGISC 164
           VTEWPLKSKLDPSIYGSPESAITDEIVEQQIKGVMSLDEGISC
Sbjct: 121 VTEWPLKSKLDPSIYGSPESAITDEIVEQQIKGVMSLDEGISC 163

[Close]

BLAST of Csa4G288110 vs. TrEMBL
Match: A0A0A0L1W4_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_4G288610 PE=4 SV=1)

HSP 1 Score: 167.5 bits (423), Expect = 1.3e-38
Identity = 95/176 (53.98%), Postives = 113/176 (64.20%), Query Frame = 1


Query: 5   DSLSEVRSMEFYVPRDEAFSISKQGNTLMKAFNSMLHGFLLPALE------DNFNKHYST 64
           D+LSE RS + YVPRDEAFS  KQ    ++   S+L G L+PALE      D    H+  
Sbjct: 287 DALSESRSSDNYVPRDEAFSPVKQATFSVRTLTSVLKG-LIPALESVSTDTDIRFPHFPA 346

Query: 65  LFETKHDRSPCP--------------KLVQILTDKAMDILLLHSSQTSYGDKFFWFRDEE 124
           + +   +  P P              +L+  + D+A DIL     +T Y DKFFWFRDEE
Sbjct: 347 IDDLYDNGVPLPAAKDGLRQLATVLPRLIDTVADRAEDILRFVPPETFYKDKFFWFRDEE 406

Query: 125 FARQTLAGLNPYSIRLVTEWPLKSKLDPSIYGSPESAITDEIVEQQIKGVMSLDEG 161
           FARQTLAGLNPYSIRLVTEWPLKSKLDP++YG P S IT EIVEQQIKG M+LDEG
Sbjct: 407 FARQTLAGLNPYSIRLVTEWPLKSKLDPAVYGDPTSKITTEIVEQQIKGFMTLDEG 461

[Close]

BLAST of Csa4G288110 vs. TrEMBL
Match: A0A125S6K7_CUCME (Lipoxygenase OS=Cucumis melo var. makuwa GN=LOX10 PE=2 SV=1)

HSP 1 Score: 164.1 bits (414), Expect = 1.5e-37
Identity = 94/177 (53.11%), Postives = 112/177 (63.28%), Query Frame = 1


Query: 5   DSLSEVRSMEFYVPRDEAFSISKQGNTLMKAFNSMLHGFLLPALE------DNFNKHYST 64
           D LSE RS + YVPRDEAFS  KQ    +K  NS+L G L+PALE      D    H+  
Sbjct: 287 DPLSESRSSDNYVPRDEAFSPVKQATFSVKTLNSLLKG-LIPALESVSTDTDIRFPHFPA 346

Query: 65  LFETKHDRSPCP--------------KLVQILTDKAMDILLLHSSQTSYGDKFFWFRDEE 124
           + +   +  P P              +L+  + D+A D L     +T   DKFFWFRDEE
Sbjct: 347 IDDLYDNGVPLPAAKGGWKQIATALPRLIDSVVDRAEDFLRFVPPETFDKDKFFWFRDEE 406

Query: 125 FARQTLAGLNPYSIRLVTEWPLKSKLDPSIYGSPESAITDEIVEQQIKGVMSLDEGI 162
           FARQTLAGLNPYSIRLVTEWPLKSKLDP++YG P S IT+EIVEQQIKG M+LDE +
Sbjct: 407 FARQTLAGLNPYSIRLVTEWPLKSKLDPAVYGDPTSKITNEIVEQQIKGFMTLDEAL 462

[Close]

BLAST of Csa4G288110 vs. TrEMBL
Match: W9R031_9ROSA (Lipoxygenase OS=Morus notabilis GN=L484_007985 PE=3 SV=1)

HSP 1 Score: 153.7 bits (387), Expect = 2.0e-34
Identity = 90/175 (51.43%), Postives = 111/175 (63.43%), Query Frame = 1


Query: 5   DSLSEVRSMEFYVPRDEAFSISKQGNTLMKAFNSMLHGFLLPALEDNFNKH-----YSTL 64
           D LSE RS   YVPRDEAFS  KQ     K   S LH  L+P+L+  F        Y T 
Sbjct: 266 DPLSEARSGSVYVPRDEAFSEVKQLTFQAKTVYSGLHA-LVPSLQTAFVNRKLGFPYFTA 325

Query: 65  FETKHD-------------RSPCPKLVQILTDKAMDILLLHSSQTSYGDKFFWFRDEEFA 124
            +T  D             ++  P+L++  +D A DIL     +T   DKFFWFRDEEFA
Sbjct: 326 IDTLFDQGLKMPSDGPGILKNVLPRLIKAASD-ADDILQFEVPETMLRDKFFWFRDEEFA 385

Query: 125 RQTLAGLNPYSIRLVTEWPLKSKLDPSIYGSPESAITDEIVEQQIKGVMSLDEGI 162
           RQTLAGLNPYSI+LVTEWPLKSKLDP+IYG PESAIT E++EQ+I+G ++++E I
Sbjct: 386 RQTLAGLNPYSIQLVTEWPLKSKLDPAIYGPPESAITTELIEQEIRGFVTVEEAI 438

[Close]

BLAST of Csa4G288110 vs. TrEMBL
Match: M5X5W8_PRUPE (Lipoxygenase OS=Prunus persica GN=PRUPE_ppa001634mg PE=3 SV=1)

HSP 1 Score: 151.4 bits (381), Expect = 9.8e-34
Identity = 88/176 (50.00%), Postives = 109/176 (61.93%), Query Frame = 1


Query: 5   DSLSEVRSMEFYVPRDEAFSISKQGNTLMKAFNSMLHGFLLPALE--------------- 64
           D LSE RS   YVPRDEAF+  K+     K   S+LH  LLP+LE               
Sbjct: 175 DPLSETRSSSVYVPRDEAFAEVKELTFSAKTLKSVLHA-LLPSLETALLNPELGFPYFTA 234

Query: 65  -DNFNKHYSTLFETKHD---RSPCPKLVQILTDKAMDILLLHSSQTSYGDKFFWFRDEEF 124
            D+      TL + K     ++  P+LV+ +TD   D+LL  + +    DKF WFRDEEF
Sbjct: 235 IDSLFNEGVTLPKPKTSGFFQTIIPRLVKTITDGGDDLLLFETPEIIDRDKFAWFRDEEF 294

Query: 125 ARQTLAGLNPYSIRLVTEWPLKSKLDPSIYGSPESAITDEIVEQQIKGVMSLDEGI 162
           +RQTLAGLNPYSI LVTEWPLKSKLDP IYG PES IT E+VE++IKG M++DE +
Sbjct: 295 SRQTLAGLNPYSIELVTEWPLKSKLDPEIYGPPESLITTELVEKEIKGCMTVDEAL 349

[Close]

BLAST of Csa4G288110 vs. TAIR10
Match: AT3G45140.1 (AT3G45140.1 lipoxygenase 2)

HSP 1 Score: 116.7 bits (291), Expect = 1.3e-26
Identity = 72/163 (44.17%), Postives = 93/163 (57.06%), Query Frame = 1


Query: 14  EFYVPRDEAFSISK----QGNTLMKAFNSM---LHGFLLPALEDNFN-KHYSTLFETKHD 73
           EFYVPRDE FS +K     G  ++ A  S+   +   LL   E   + K    LFE    
Sbjct: 294 EFYVPRDEEFSTAKGTSFTGKAVLAALPSIFPQIESVLLSPQEPFPHFKAIQNLFEEGIQ 353

Query: 74  RSP-------CPKLVQILTDKAMDILLLHSSQTSYGDKFFWFRDEEFARQTLAGLNPYSI 133
                      P++++ L +   DIL   +      D+F W RD+EFARQTLAGLNPYSI
Sbjct: 354 LPKDAGLLPLLPRIIKALGEAQDDILQFDAPVLINRDRFSWLRDDEFARQTLAGLNPYSI 413

Query: 134 RLVTEWPLKSKLDPSIYGSPESAITDEIVEQQIKGVMSLDEGI 162
           +LV EWPL SKLDP++YG P S IT EIVE+++KG M++DE +
Sbjct: 414 QLVEEWPLISKLDPAVYGDPTSLITWEIVEREVKGNMTVDEAL 456

[Close]

BLAST of Csa4G288110 vs. TAIR10
Match: AT1G67560.1 (AT1G67560.1 PLAT/LH2 domain-containing lipoxygenase family protein)

HSP 1 Score: 79.7 bits (195), Expect = 1.8e-15
Identity = 49/159 (30.82%), Postives = 76/159 (47.80%), Query Frame = 1


Query: 14  EFYVPRDEAFSISKQGNTLMKAFNSMLHGFLLPALE----------------DNFNKHYS 73
           EFYVPRDE F   K+       F ++ H  L+P++                 DN  K   
Sbjct: 309 EFYVPRDEVFEEIKRDTFRAGRFKALFHN-LVPSIAAALSNLDIPFTCFSDIDNLYKSNI 368

Query: 74  TLFETKHDRSPCPKLVQILTDKAMDI----LLLHSSQTSYGDKFFWFRDEEFARQTLAGL 133
            L  T+   +     +    +  +++    L   +      D+F W RD EF RQ LAG+
Sbjct: 369 VLGHTEPKDTGLGGFIGGFMNGILNVTETLLKYDTPAVIKWDRFAWLRDNEFGRQALAGV 428

Query: 134 NPYSIRLVTEWPLKSKLDPSIYGSPESAITDEIVEQQIK 153
           NP +I L+ E P++S LDP++YG  ES +T+EI+ ++++
Sbjct: 429 NPVNIELLKELPIRSNLDPALYGPQESVLTEEIIAREVE 466

[Close]

BLAST of Csa4G288110 vs. TAIR10
Match: AT1G55020.1 (AT1G55020.1 lipoxygenase 1)

HSP 1 Score: 77.8 bits (190), Expect = 6.9e-15
Identity = 57/173 (32.95%), Postives = 75/173 (43.35%), Query Frame = 1


Query: 3   TVDSLSEVRSMEFYVPRDEAFSISKQGNTLMKAFNSMLHGFLLPALE---DNFNKHYST- 62
           T   L    S++ YVPRDE F   K  + L  A  ++   F+ PALE   D+  K + + 
Sbjct: 244 TESRLPITSSLDIYVPRDERFGHLKMSDFLAYALKAIAQ-FIQPALEAVFDDTPKEFDSF 303

Query: 63  -----------------LFETKHDRSPCPKLVQILTDKAMDILLLHSSQTSYGDKFFWFR 122
                            L ++     P   L +I        L     Q    DK  W  
Sbjct: 304 EDVLKIYEEGIDLPNQALIDSIVKNIPLEMLKEIFRTDGQKFLKFPVPQVIKEDKTAWRT 363

Query: 123 DEEFARQTLAGLNPYSIRLVTEWPLKSKLDPSIYGSPESAITDEIVEQQIKGV 155
           DEEFAR+ LAGLNP  I+L+ E+P KSKLD   YG+  S IT   +E  + G+
Sbjct: 364 DEEFAREMLAGLNPVVIQLLKEFPPKSKLDSESYGNQNSTITKSHIEHNLDGL 415

[Close]

BLAST of Csa4G288110 vs. TAIR10
Match: AT1G72520.1 (AT1G72520.1 PLAT/LH2 domain-containing lipoxygenase family protein)

HSP 1 Score: 72.8 bits (177), Expect = 2.2e-13
Identity = 56/171 (32.75%), Postives = 80/171 (46.78%), Query Frame = 1


Query: 5   DSLSEVR---SMEFYVPRDEAFSISKQG-----------NTLMKAFNSML--HGFLLPAL 64
           D +SE R    +  YVPRDE F  SKQ            + L+ +  + +    F     
Sbjct: 310 DMMSERRVEKPLPMYVPRDEQFEESKQNTFAACRLKAVLHNLIPSLKASILAEDFANFGE 369

Query: 65  EDNFNKHYSTLFETKHD----RSPCPKLVQILTDKAMDILLLHSSQTSYGDKFFWFRDEE 124
            D+  K    L     D    + P PK+V  L   +  +L   + +    DK+ W RD+E
Sbjct: 370 IDSLYKEGLLLKLGFQDDMFKKFPLPKIVTTLQKSSEGLLRYDTPKIVSKDKYAWLRDDE 429

Query: 125 FARQTLAGLNPYSIRLVTEWPLKSKLDPSIYG-SPESAITDEIVEQQIKGV 155
           FARQ +AG+NP +I  VT +P  S LDP IYG    SA+T++ +  Q+ G+
Sbjct: 430 FARQAIAGINPVNIERVTSYPPVSNLDPEIYGPGLHSALTEDHIIGQLDGL 480

[Close]

BLAST of Csa4G288110 vs. TAIR10
Match: AT1G17420.1 (AT1G17420.1 lipoxygenase 3)

HSP 1 Score: 67.4 bits (163), Expect = 9.4e-12
Identity = 50/161 (31.06%), Postives = 69/161 (42.86%), Query Frame = 1


Query: 11  RSMEFYVPRDEAFSISKQGN--------TLMKAFNSMLHGFLLPALED--NFNKHYSTLF 70
           + +  YVPRDE F  SKQ           L     S+    +     D    ++ Y    
Sbjct: 313 KPLPMYVPRDEQFEESKQDTFAAGRLKAVLHHLIPSLKASIVAEDFADFGEIDRLYKEGL 372

Query: 71  ETK-------HDRSPCPKLV-QILTDKAMDILLLHSSQTSYGDKFFWFRDEEFARQTLAG 130
             K         + P PK+V   L +    +L   + +    DK  W RD+EFARQ +AG
Sbjct: 373 LLKLGFQDDIFKKFPLPKVVVDTLQESTKGLLKYDTPKILSKDKNAWLRDDEFARQAIAG 432

Query: 131 LNPYSIRLVTEWPLKSKLDPSIYGSPESAITDEIVEQQIKG 154
           +NP +I  V  +P  S LDP IYG   SA+TD+ +   + G
Sbjct: 433 INPVNIERVKTFPPVSNLDPKIYGPQHSALTDDHIIGHLDG 473

[Close]

BLAST of Csa4G288110 vs. NCBI nr
Match: gi|700198982|gb|KGN54140.1| (hypothetical protein Csa_4G288110 [Cucumis sativus])

HSP 1 Score: 330.5 bits (846), Expect = 1.7e-87
Identity = 163/163 (100.00%), Postives = 163/163 (100.00%), Query Frame = 1


Query: 1   MLTVDSLSEVRSMEFYVPRDEAFSISKQGNTLMKAFNSMLHGFLLPALEDNFNKHYSTLF 60
           MLTVDSLSEVRSMEFYVPRDEAFSISKQGNTLMKAFNSMLHGFLLPALEDNFNKHYSTLF
Sbjct: 1   MLTVDSLSEVRSMEFYVPRDEAFSISKQGNTLMKAFNSMLHGFLLPALEDNFNKHYSTLF 60

Query: 61  ETKHDRSPCPKLVQILTDKAMDILLLHSSQTSYGDKFFWFRDEEFARQTLAGLNPYSIRL 120
           ETKHDRSPCPKLVQILTDKAMDILLLHSSQTSYGDKFFWFRDEEFARQTLAGLNPYSIRL
Sbjct: 61  ETKHDRSPCPKLVQILTDKAMDILLLHSSQTSYGDKFFWFRDEEFARQTLAGLNPYSIRL 120

Query: 121 VTEWPLKSKLDPSIYGSPESAITDEIVEQQIKGVMSLDEGISC 164
           VTEWPLKSKLDPSIYGSPESAITDEIVEQQIKGVMSLDEGISC
Sbjct: 121 VTEWPLKSKLDPSIYGSPESAITDEIVEQQIKGVMSLDEGISC 163

[Close]

BLAST of Csa4G288110 vs. NCBI nr
Match: gi|449448964|ref|XP_004142235.1| (PREDICTED: linoleate 13S-lipoxygenase 2-1, chloroplastic-like [Cucumis sativus])

HSP 1 Score: 315.5 bits (807), Expect = 5.6e-83
Identity = 155/157 (98.73%), Postives = 156/157 (99.36%), Query Frame = 1


Query: 5   DSLSEVRSMEFYVPRDEAFSISKQGNTLMKAFNSMLHGFLLPALEDNFNKHYSTLFETKH 64
           DSLSEVRSMEFYVPRDEAFSISKQGNTLMKAFNSMLHGFLLPALEDNFNKHYSTLFETKH
Sbjct: 224 DSLSEVRSMEFYVPRDEAFSISKQGNTLMKAFNSMLHGFLLPALEDNFNKHYSTLFETKH 283

Query: 65  DRSPCPKLVQILTDKAMDILLLHSSQTSYGDKFFWFRDEEFARQTLAGLNPYSIRLVTEW 124
           DRSPCPKLVQILTDKAMDILLLHSSQTSYGDKFFWFRDEEFARQTLAGLNPYSIRLVTEW
Sbjct: 284 DRSPCPKLVQILTDKAMDILLLHSSQTSYGDKFFWFRDEEFARQTLAGLNPYSIRLVTEW 343

Query: 125 PLKSKLDPSIYGSPESAITDEIVEQQIKGVMSLDEGI 162
           PLKSKLDPSIYGSPESAITDEIVEQQIKGVMSLDE +
Sbjct: 344 PLKSKLDPSIYGSPESAITDEIVEQQIKGVMSLDEAL 380

[Close]

BLAST of Csa4G288110 vs. NCBI nr
Match: gi|659098038|ref|XP_008449946.1| (PREDICTED: linoleate 13S-lipoxygenase 2-1, chloroplastic-like [Cucumis melo])

HSP 1 Score: 266.2 bits (679), Expect = 3.9e-68
Identity = 137/157 (87.26%), Postives = 145/157 (92.36%), Query Frame = 1


Query: 5   DSLSEVRSMEFYVPRDEAFSISKQGNTLMKAFNSMLHGFLLPALEDNFNKHYSTLFETKH 64
           D LSEVRSMEFYVPRDEAFSIS+QGNTLMKAF SML+GFL PALE+ F +H S LFETKH
Sbjct: 283 DPLSEVRSMEFYVPRDEAFSISEQGNTLMKAFYSMLYGFL-PALENIFTEHNSNLFETKH 342

Query: 65  DRSPCPKLVQILTDKAMDILLLHSSQTSYGDKFFWFRDEEFARQTLAGLNPYSIRLVTEW 124
             SP  +LVQILTDKAMDILLLHSSQTSYGDKFFWFRDEEFARQTLAGLNPYSIRLVTEW
Sbjct: 343 P-SPFARLVQILTDKAMDILLLHSSQTSYGDKFFWFRDEEFARQTLAGLNPYSIRLVTEW 402

Query: 125 PLKSKLDPSIYGSPESAITDEIVEQQIKGVMSLDEGI 162
           PLKS+LDPSIYGSPESAITDEIVEQQIKG+MSLDE +
Sbjct: 403 PLKSELDPSIYGSPESAITDEIVEQQIKGIMSLDEAL 437

[Close]

BLAST of Csa4G288110 vs. NCBI nr
Match: gi|700198983|gb|KGN54141.1| (hypothetical protein Csa_4G288610 [Cucumis sativus])

HSP 1 Score: 167.5 bits (423), Expect = 1.9e-38
Identity = 95/176 (53.98%), Postives = 113/176 (64.20%), Query Frame = 1


Query: 5   DSLSEVRSMEFYVPRDEAFSISKQGNTLMKAFNSMLHGFLLPALE------DNFNKHYST 64
           D+LSE RS + YVPRDEAFS  KQ    ++   S+L G L+PALE      D    H+  
Sbjct: 287 DALSESRSSDNYVPRDEAFSPVKQATFSVRTLTSVLKG-LIPALESVSTDTDIRFPHFPA 346

Query: 65  LFETKHDRSPCP--------------KLVQILTDKAMDILLLHSSQTSYGDKFFWFRDEE 124
           + +   +  P P              +L+  + D+A DIL     +T Y DKFFWFRDEE
Sbjct: 347 IDDLYDNGVPLPAAKDGLRQLATVLPRLIDTVADRAEDILRFVPPETFYKDKFFWFRDEE 406

Query: 125 FARQTLAGLNPYSIRLVTEWPLKSKLDPSIYGSPESAITDEIVEQQIKGVMSLDEG 161
           FARQTLAGLNPYSIRLVTEWPLKSKLDP++YG P S IT EIVEQQIKG M+LDEG
Sbjct: 407 FARQTLAGLNPYSIRLVTEWPLKSKLDPAVYGDPTSKITTEIVEQQIKGFMTLDEG 461

[Close]

BLAST of Csa4G288110 vs. NCBI nr
Match: gi|778693116|ref|XP_004142135.2| (PREDICTED: linoleate 13S-lipoxygenase 2-1, chloroplastic-like [Cucumis sativus])

HSP 1 Score: 166.0 bits (419), Expect = 5.5e-38
Identity = 94/177 (53.11%), Postives = 113/177 (63.84%), Query Frame = 1


Query: 5   DSLSEVRSMEFYVPRDEAFSISKQGNTLMKAFNSMLHGFLLPALE------DNFNKHYST 64
           D+LSE RS + YVPRDEAFS  KQ    ++   S+L G L+PALE      D    H+  
Sbjct: 287 DALSESRSSDNYVPRDEAFSPVKQATFSVRTLTSVLKG-LIPALESVSTDTDIRFPHFPA 346

Query: 65  LFETKHDRSPCP--------------KLVQILTDKAMDILLLHSSQTSYGDKFFWFRDEE 124
           + +   +  P P              +L+  + D+A DIL     +T Y DKFFWFRDEE
Sbjct: 347 IDDLYDNGVPLPAAKDGLRQLATVLPRLIDTVADRAEDILRFVPPETFYKDKFFWFRDEE 406

Query: 125 FARQTLAGLNPYSIRLVTEWPLKSKLDPSIYGSPESAITDEIVEQQIKGVMSLDEGI 162
           FARQTLAGLNPYSIRLVTEWPLKSKLDP++YG P S IT EIVEQQIKG M+LDE +
Sbjct: 407 FARQTLAGLNPYSIRLVTEWPLKSKLDPAVYGDPTSKITTEIVEQQIKGFMTLDEAL 462

The following BLAST results are available for this feature:

BLAST of Csa4G288110 vs. Swiss-Prot


Match Name	E-value	Identity	Description
LOX21_SOLTU	5.9e-32	45.86	Linoleate 13S-lipoxygenase 2-1, chloroplastic OS=Solanum tuberosum GN=LOX2.1 PE=...	[more]
LOXC2_ORYSJ	3.0e-28	43.33	Probable lipoxygenase 8, chloroplastic OS=Oryza sativa subsp. japonica GN=CM-LOX...	[more]
LOX2_ARATH	2.4e-25	44.17	Lipoxygenase 2, chloroplastic OS=Arabidopsis thaliana GN=LOX2 PE=1 SV=1	[more]
LOXC1_ORYSJ	1.0e-23	42.22	Lipoxygenase 7, chloroplastic OS=Oryza sativa subsp. japonica GN=CM-LOX1 PE=2 SV...	[more]
LOX23_HORVU	1.3e-23	37.99	Lipoxygenase 2.3, chloroplastic OS=Hordeum vulgare GN=LOX2.3 PE=1 SV=1	[more]

BLAST of Csa4G288110 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KWY8_CUCSA	1.2e-87	100.00	Uncharacterized protein OS=Cucumis sativus GN=Csa_4G288110 PE=4 SV=1	[more]
A0A0A0L1W4_CUCSA	1.3e-38	53.98	Uncharacterized protein OS=Cucumis sativus GN=Csa_4G288610 PE=4 SV=1	[more]
A0A125S6K7_CUCME	1.5e-37	53.11	Lipoxygenase OS=Cucumis melo var. makuwa GN=LOX10 PE=2 SV=1	[more]
W9R031_9ROSA	2.0e-34	51.43	Lipoxygenase OS=Morus notabilis GN=L484_007985 PE=3 SV=1	[more]
M5X5W8_PRUPE	9.8e-34	50.00	Lipoxygenase OS=Prunus persica GN=PRUPE_ppa001634mg PE=3 SV=1	[more]

BLAST of Csa4G288110 vs. TAIR10


Match Name	E-value	Identity	Description
AT3G45140.1	1.3e-26	44.17	lipoxygenase 2	[more]
AT1G67560.1	1.8e-15	30.82	PLAT/LH2 domain-containing lipoxygenase family protein	[more]
AT1G55020.1	6.9e-15	32.95	lipoxygenase 1	[more]
AT1G72520.1	2.2e-13	32.75	PLAT/LH2 domain-containing lipoxygenase family protein	[more]
AT1G17420.1	9.4e-12	31.06	lipoxygenase 3	[more]

BLAST of Csa4G288110 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|700198982\|gb\|KGN54140.1\|	1.7e-87	100.00	hypothetical protein Csa_4G288110 [Cucumis sativus]	[more]
gi\|449448964\|ref\|XP_004142235.1\|	5.6e-83	98.73	PREDICTED: linoleate 13S-lipoxygenase 2-1, chloroplastic-like [Cucumis sativus]	[more]
gi\|659098038\|ref\|XP_008449946.1\|	3.9e-68	87.26	PREDICTED: linoleate 13S-lipoxygenase 2-1, chloroplastic-like [Cucumis melo]	[more]
gi\|700198983\|gb\|KGN54141.1\|	1.9e-38	53.98	hypothetical protein Csa_4G288610 [Cucumis sativus]	[more]
gi\|778693116\|ref\|XP_004142135.2\|	5.5e-38	53.11	PREDICTED: linoleate 13S-lipoxygenase 2-1, chloroplastic-like [Cucumis sativus]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR000907	LipOase
IPR013819	LipOase_C
IPR027433	Lipoxygenase_dom_3

Vocabulary: **Molecular Function**
Term	Definition
GO:0016702	oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen
GO:0046872	metal ion binding

Vocabulary: **Biological Process**
Term	Definition
GO:0055114	oxidation-reduction process

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0055114	oxidation-reduction process
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0046872	metal ion binding
molecular_function	GO:0016702	oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen

Unigene

This gene is associated with the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
CU135630	cucumber EST collection version 3.0	transcribed_cluster

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Csa4G288110.1	Csa4G288110.1	mRNA

The following transcribed_cluster feature(s) are associated with this gene:


Feature Name	Unique Name	Type
CU135630	CU135630	transcribed_cluster

InterPro

Analysis Name: InterPro Annotations of cucumber (Chinese Long)
Date Performed: 2016-09-28


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000907	Lipoxygenase	PANTHER	PTHR11771	LIPOXYGENASE	coord: 5..63 score: 2.0E-35coord: 81..162 score: 2.0
IPR013819	Lipoxygenase, C-terminal	PFAM	PF00305	Lipoxygenase	coord: 8..161 score: 4.3
IPR013819	Lipoxygenase, C-terminal	PROFILE	PS51393	LIPOXYGENASE_3	coord: 1..163 score: 32
IPR013819	Lipoxygenase, C-terminal	unknown	SSF48484	Lipoxigenase	coord: 5..159 score: 5.76
IPR027433	Lipoxygenase, domain 3	GENE3D	G3DSA:4.10.372.10		coord: 52..115 score: 4.3
None	No IPR available	GENE3D	G3DSA:3.10.450.60		coord: 117..160 score: 2.
None	No IPR available	GENE3D	G3DSA:4.10.375.10		coord: 5..42 score: 1.
None	No IPR available	PANTHER	PTHR11771:SF42	PROTEIN C13C4.7	coord: 81..162 score: 2.0E-35coord: 5..63 score: 2.0

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Csa4G288110	Carg06923	Silver-seed gourd	carcuB0552

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Csa4G288110	Cucumber (Gy14) v2	cgybcuB167
Csa4G288110	Melon (DHL92) v3.6.1	cumedB279
Csa4G288110	Watermelon (97103) v2	cuwmbB287
Csa4G288110	Wax gourd	cuwgoB390
Csa4G288110	Cucumber (Gy14) v1	cgycuB055
Csa4G288110	Cucurbita maxima (Rimu)	cmacuB549
Csa4G288110	Cucurbita moschata (Rifu)	cmocuB538
Csa4G288110	Cucurbita moschata (Rifu)	cmocuB596
Csa4G288110	Melon (DHL92) v3.5.1	cumeB295
Csa4G288110	Watermelon (Charleston Gray)	cuwcgB292
Csa4G288110	Watermelon (97103) v1	cuwmB347
Csa4G288110	Bottle gourd (USVL1VR-Ls)	culsiB246