Csa2G008700 (gene) Cucumber (Chinese Long) v2

NameCsa2G008700
Typegene
OrganismCucumis. sativus (Cucumber (Chinese Long) v2)
DescriptionPutative nuclease HARBI1; contains IPR026103 (Harbinger transposase-derived nuclease)
LocationChr2 : 1485458 .. 1487037 (+)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCATTCACAGTGTGTGCTCCCCTACCCTGTTCGCCATGGCCACCAGAGGACTCGCCGGGGACAAGAGAACCACCAGAAGTTCCGCCATGAACGCTGCCGCCGCCGCCATTACCAGAAGCAAGGCCAAGAAACTCGATCAAGAGAACCATCTTAACCATCAACTGATAACCCTCATCGAAACCACCATTTCTTCTGCTCACTCCTTTCTCTCTCTCAACGATCTTCACCTTCTTCCCTCTCAAACCCTCGCCCTTGAATCCCTACTTTGTTCCACTTCATCTTCTCTTCACGCTCTTTCTCCTCGTCTCCCAAAACTTTCCCTACCTCCGCCACTACCTCCACCGCGCCAATGCTGGTTCCAGCGCTTCCTATCCGCGACATCGGACGTCGATTGCGATCCGAGATGGAATCTCTCTTTCCGTATGTCGAAATCCTCTTTCTCCCTCCTCCTTCGTCTCCTTTCTCCGATTCAAAGCTCCCCATCCTCTTCAGTTCCTCCCGATTGTGCTTTAGCTGCTGCGCTTTTCCGATTGGCGCATGGCGCGAGCTACAAGGCGGTTGGGAGACGGTTTGGGATCGATTCTGCTGATGCTTGTCGGTCGTTTTATGCTGTTTGTAAAGCTATTAATGAGAAATTGGGGCATTTGCTTGAGTTACGGTCTGACATTGATCGGATTGTTGTGGGATTTGGGTGGATTTCGCTTCCGAATTGTTGTGGGGTTTTAGGTCTAAGAAGATTTGGGTTTGAAGGTGAGCTGAAAAATGGATCGCTTCTGGTTCAAGCATTAGTCGATGCTGAAGGGAGGTTTCTGGATGTCTCTGCTGGTTGGCCGAGCTCCATGAAACCTGCAACAATCTTGCGGCAGAGCAAACTATATGCAGAAATTGAGAAATCTAGTGAATTACTGAAGGGTCCTGTTTATAATCTCGACAATGAAAAACCCATTCCCCAATACTTGATCGGTGATTCTTGCTTCCCCCTTTTGCCATGGCTTTTGACACCATATATGGAACTGAATGAAGAAGATAGTTCTGGCTTTTGTGGGAGAGCATTCAATTCCACACATGGCCGTGCAATGGCGTTGGTTAACACAGCATTTTGCAGACTCCGAGCTCGGTGGAAGCTCTTGTCAAAACCATGGAAGGAAGGATGTAGAGATTTTTTCCCATTTATTATATTGACTGGATGTCTGCTGCAGAATTTCCTGATTAAATGCAGTGAGAAACTAGATGAAGAGCAAGATCAAGAAGAAGGAGCAAGTTGCTCAAGTGAGGAGCAAAAGTTTCCTCTTTTCGATGGTGAGATAGGAGATGGTAGAGGAAAGGATATCAGAGATGCCCTTGCCTTGCACTTGAGTAGCCTGAACTACAGAAGATGATTGCTTTGAACACCTTGGTAATTTTAATCTTTCATCAGCTGTATATATTCCTCTCTGATCCTTTAGAACATTTTGTTAAAACTGCAGATTGTAAGCCCTAATTCTCTAGAAGTAGATCTTTTTTTTTTTTTTGTCAGCCATTAGTTTCCTAGTGATTTGATTCTTATTGTTAGAGAAGGGATGTTTACACTTATCGAA

mRNA sequence

ATGGCCACCAGAGGACTCGCCGGGGACAAGAGAACCACCAGAAGTTCCGCCATGAACGCTGCCGCCGCCGCCATTACCAGAAGCAAGGCCAAGAAACTCGATCAAGAGAACCATCTTAACCATCAACTGATAACCCTCATCGAAACCACCATTTCTTCTGCTCACTCCTTTCTCTCTCTCAACGATCTTCACCTTCTTCCCTCTCAAACCCTCGCCCTTGAATCCCTACTTTGTTCCACTTCATCTTCTCTTCACGCTCTTTCTCCTCGTCTCCCAAAACTTTCCCTACCTCCGCCACTACCTCCACCGCGCCAATGCTGGTTCCAGCGCTTCCTATCCGCGACATCGGACGTCGATTGCGATCCGAGATGGAATCTCTCTTTCCGTATGTCGAAATCCTCTTTCTCCCTCCTCCTTCGTCTCCTTTCTCCGATTCAAAGCTCCCCATCCTCTTCAGTTCCTCCCGATTGTGCTTTAGCTGCTGCGCTTTTCCGATTGGCGCATGGCGCGAGCTACAAGGCGGTTGGGAGACGGTTTGGGATCGATTCTGCTGATGCTTGTCGGTCGTTTTATGCTGTTTGTAAAGCTATTAATGAGAAATTGGGGCATTTGCTTGAGTTACGGTCTGACATTGATCGGATTGTTGTGGGATTTGGGTGGATTTCGCTTCCGAATTGTTGTGGGGTTTTAGGTCTAAGAAGATTTGGGTTTGAAGGTGAGCTGAAAAATGGATCGCTTCTGGTTCAAGCATTAGTCGATGCTGAAGGGAGGTTTCTGGATGTCTCTGCTGGTTGGCCGAGCTCCATGAAACCTGCAACAATCTTGCGGCAGAGCAAACTATATGCAGAAATTGAGAAATCTAGTGAATTACTGAAGGGTCCTGTTTATAATCTCGACAATGAAAAACCCATTCCCCAATACTTGATCGGTGATTCTTGCTTCCCCCTTTTGCCATGGCTTTTGACACCATATATGGAACTGAATGAAGAAGATAGTTCTGGCTTTTGTGGGAGAGCATTCAATTCCACACATGGCCGTGCAATGGCGTTGGTTAACACAGCATTTTGCAGACTCCGAGCTCGGTGGAAGCTCTTGTCAAAACCATGGAAGGAAGGATGTAGAGATTTTTTCCCATTTATTATATTGACTGGATGTCTGCTGCAGAATTTCCTGATTAAATGCAGTGAGAAACTAGATGAAGAGCAAGATCAAGAAGAAGGAGCAAGTTGCTCAAGTGAGGAGCAAAAGTTTCCTCTTTTCGATGGTGAGATAGGAGATGGTAGAGGAAAGGATATCAGAGATGCCCTTGCCTTGCACTTGAGTAGCCTGAACTACAGAAGATGA

Coding sequence (CDS)

ATGGCCACCAGAGGACTCGCCGGGGACAAGAGAACCACCAGAAGTTCCGCCATGAACGCTGCCGCCGCCGCCATTACCAGAAGCAAGGCCAAGAAACTCGATCAAGAGAACCATCTTAACCATCAACTGATAACCCTCATCGAAACCACCATTTCTTCTGCTCACTCCTTTCTCTCTCTCAACGATCTTCACCTTCTTCCCTCTCAAACCCTCGCCCTTGAATCCCTACTTTGTTCCACTTCATCTTCTCTTCACGCTCTTTCTCCTCGTCTCCCAAAACTTTCCCTACCTCCGCCACTACCTCCACCGCGCCAATGCTGGTTCCAGCGCTTCCTATCCGCGACATCGGACGTCGATTGCGATCCGAGATGGAATCTCTCTTTCCGTATGTCGAAATCCTCTTTCTCCCTCCTCCTTCGTCTCCTTTCTCCGATTCAAAGCTCCCCATCCTCTTCAGTTCCTCCCGATTGTGCTTTAGCTGCTGCGCTTTTCCGATTGGCGCATGGCGCGAGCTACAAGGCGGTTGGGAGACGGTTTGGGATCGATTCTGCTGATGCTTGTCGGTCGTTTTATGCTGTTTGTAAAGCTATTAATGAGAAATTGGGGCATTTGCTTGAGTTACGGTCTGACATTGATCGGATTGTTGTGGGATTTGGGTGGATTTCGCTTCCGAATTGTTGTGGGGTTTTAGGTCTAAGAAGATTTGGGTTTGAAGGTGAGCTGAAAAATGGATCGCTTCTGGTTCAAGCATTAGTCGATGCTGAAGGGAGGTTTCTGGATGTCTCTGCTGGTTGGCCGAGCTCCATGAAACCTGCAACAATCTTGCGGCAGAGCAAACTATATGCAGAAATTGAGAAATCTAGTGAATTACTGAAGGGTCCTGTTTATAATCTCGACAATGAAAAACCCATTCCCCAATACTTGATCGGTGATTCTTGCTTCCCCCTTTTGCCATGGCTTTTGACACCATATATGGAACTGAATGAAGAAGATAGTTCTGGCTTTTGTGGGAGAGCATTCAATTCCACACATGGCCGTGCAATGGCGTTGGTTAACACAGCATTTTGCAGACTCCGAGCTCGGTGGAAGCTCTTGTCAAAACCATGGAAGGAAGGATGTAGAGATTTTTTCCCATTTATTATATTGACTGGATGTCTGCTGCAGAATTTCCTGATTAAATGCAGTGAGAAACTAGATGAAGAGCAAGATCAAGAAGAAGGAGCAAGTTGCTCAAGTGAGGAGCAAAAGTTTCCTCTTTTCGATGGTGAGATAGGAGATGGTAGAGGAAAGGATATCAGAGATGCCCTTGCCTTGCACTTGAGTAGCCTGAACTACAGAAGATGA

Protein sequence

MATRGLAGDKRTTRSSAMNAAAAAITRSKAKKLDQENHLNHQLITLIETTISSAHSFLSLNDLHLLPSQTLALESLLCSTSSSLHALSPRLPKLSLPPPLPPPRQCWFQRFLSATSDVDCDPRWNLSFRMSKSSFSLLLRLLSPIQSSPSSSVPPDCALAAALFRLAHGASYKAVGRRFGIDSADACRSFYAVCKAINEKLGHLLELRSDIDRIVVGFGWISLPNCCGVLGLRRFGFEGELKNGSLLVQALVDAEGRFLDVSAGWPSSMKPATILRQSKLYAEIEKSSELLKGPVYNLDNEKPIPQYLIGDSCFPLLPWLLTPYMELNEEDSSGFCGRAFNSTHGRAMALVNTAFCRLRARWKLLSKPWKEGCRDFFPFIILTGCLLQNFLIKCSEKLDEEQDQEEGASCSSEEQKFPLFDGEIGDGRGKDIRDALALHLSSLNYRR*
BLAST of Csa2G008700 vs. Swiss-Prot
Match: HARB1_RAT (Putative nuclease HARBI1 OS=Rattus norvegicus GN=Harbi1 PE=2 SV=1)

HSP 1 Score: 63.2 bits (152), Expect = 8.6e-09
Identity = 67/277 (24.19%), Postives = 102/277 (36.82%), Query Frame = 1

Query: 138 LLRLLSPIQSSP---SSSVPPDCALAAALFRLAHGASYKAVGRRFGIDSADACRSFYAVC 197
           L+ LL    S P   S ++ P+  + AAL     G+    +G   GI  A   R    V 
Sbjct: 49  LVELLGASLSRPTQRSRAISPETQILAALGFYTSGSFQTRMGDAIGISQASMSRCVANVT 108

Query: 198 KAINEKLGHLLELRSDIDRIVV----GFGWISLPNCCGVLGLRRFGFEGELKNG------ 257
           +A+ E+    +   +D   I       +G   +P   G +       +            
Sbjct: 109 EALVERASQFIHFPADEAAIQSLKDEFYGLAGMPGVIGAVDCIHVAIKAPNAEDLSYVNR 168

Query: 258 ----SLLVQALVDAEGRFLDVSAGWPSSMKPATILRQSKLYAEIEKSSELLKGPVYNLDN 317
               SL    + D  G  + V   WP S++   +L+QS L ++ E               
Sbjct: 169 KGLHSLNCLVVCDIRGALMTVETSWPGSLQDCAVLQQSSLSSQFETGM------------ 228

Query: 318 EKPIPQYLIGDSCFPLLPWLLTPYMELNEEDSSGFCGRAFNSTHGRAMALVNTAFCRLR- 377
             P   +L+GDS F L  WLLTP + + E  +     RA ++TH      + T  CR R 
Sbjct: 229 --PKDSWLLGDSSFFLHTWLLTP-LHIPETPAEYRYNRAHSATHSVIEKTLRTLCCRFRC 288

Query: 378 ---ARWKLLSKPWKEGCRDFFPFIILTGCLLQNFLIK 394
              ++  L   P K         IIL  C+L N  ++
Sbjct: 289 LDGSKGALQYSPEKSS------HIILACCVLHNISLE 304

BLAST of Csa2G008700 vs. Swiss-Prot
Match: HARB1_MOUSE (Putative nuclease HARBI1 OS=Mus musculus GN=Harbi1 PE=2 SV=1)

HSP 1 Score: 60.5 bits (145), Expect = 5.6e-08
Identity = 66/275 (24.00%), Postives = 99/275 (36.00%), Query Frame = 1

Query: 138 LLRLLSPIQSSP---SSSVPPDCALAAALFRLAHGASYKAVGRRFGIDSADACRSFYAVC 197
           L+ LL    S P   S ++ P+  + AAL     G+    +G   GI  A   R    V 
Sbjct: 49  LVELLGASLSRPTQRSRAISPETQILAALGFYTSGSFQTRMGDAIGISQASMSRCVANVT 108

Query: 198 KAINEKLGHLLELRSDIDRIVVG------FGWISLPNCCGVLGLRRFGFEGELKNG---- 257
           +A+ E+    +     +D   V       +G   +P   GV        +          
Sbjct: 109 EALVERASQFIHF--PVDEAAVQSLKDEFYGLAGMPGVIGVADCIHVAIKAPNAEDLSYV 168

Query: 258 ------SLLVQALVDAEGRFLDVSAGWPSSMKPATILRQSKLYAEIEKSSELLKGPVYNL 317
                 SL    + D  G  + V   WP S++   +L++S L ++ E             
Sbjct: 169 NRKGLHSLNCLVVCDIRGALMTVETSWPGSLQDCAVLQRSSLTSQFETGM---------- 228

Query: 318 DNEKPIPQYLIGDSCFPLLPWLLTPYMELNEEDSSGFCGRAFNSTHGRAMALVNTAFCRL 377
               P   +L+GDS F L  WLLTP + + E  +     RA ++TH      + T  CR 
Sbjct: 229 ----PKDSWLLGDSSFFLRSWLLTP-LPIPETAAEYRYNRAHSATHSVIERTLQTLCCRF 288

Query: 378 RARWKLLSKPWKEGCRDFFP----FIILTGCLLQN 390
           R           +G   + P     IIL  C+L N
Sbjct: 289 RC------LDGSKGALQYSPEKCSHIILACCVLHN 300

BLAST of Csa2G008700 vs. TrEMBL
Match: A0A0A0LFB5_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G008700 PE=4 SV=1)

HSP 1 Score: 902.5 bits (2331), Expect = 2.0e-259
Identity = 447/447 (100.00%), Postives = 447/447 (100.00%), Query Frame = 1

Query: 1   MATRGLAGDKRTTRSSAMNAAAAAITRSKAKKLDQENHLNHQLITLIETTISSAHSFLSL 60
           MATRGLAGDKRTTRSSAMNAAAAAITRSKAKKLDQENHLNHQLITLIETTISSAHSFLSL
Sbjct: 1   MATRGLAGDKRTTRSSAMNAAAAAITRSKAKKLDQENHLNHQLITLIETTISSAHSFLSL 60

Query: 61  NDLHLLPSQTLALESLLCSTSSSLHALSPRLPKLSLPPPLPPPRQCWFQRFLSATSDVDC 120
           NDLHLLPSQTLALESLLCSTSSSLHALSPRLPKLSLPPPLPPPRQCWFQRFLSATSDVDC
Sbjct: 61  NDLHLLPSQTLALESLLCSTSSSLHALSPRLPKLSLPPPLPPPRQCWFQRFLSATSDVDC 120

Query: 121 DPRWNLSFRMSKSSFSLLLRLLSPIQSSPSSSVPPDCALAAALFRLAHGASYKAVGRRFG 180
           DPRWNLSFRMSKSSFSLLLRLLSPIQSSPSSSVPPDCALAAALFRLAHGASYKAVGRRFG
Sbjct: 121 DPRWNLSFRMSKSSFSLLLRLLSPIQSSPSSSVPPDCALAAALFRLAHGASYKAVGRRFG 180

Query: 181 IDSADACRSFYAVCKAINEKLGHLLELRSDIDRIVVGFGWISLPNCCGVLGLRRFGFEGE 240
           IDSADACRSFYAVCKAINEKLGHLLELRSDIDRIVVGFGWISLPNCCGVLGLRRFGFEGE
Sbjct: 181 IDSADACRSFYAVCKAINEKLGHLLELRSDIDRIVVGFGWISLPNCCGVLGLRRFGFEGE 240

Query: 241 LKNGSLLVQALVDAEGRFLDVSAGWPSSMKPATILRQSKLYAEIEKSSELLKGPVYNLDN 300
           LKNGSLLVQALVDAEGRFLDVSAGWPSSMKPATILRQSKLYAEIEKSSELLKGPVYNLDN
Sbjct: 241 LKNGSLLVQALVDAEGRFLDVSAGWPSSMKPATILRQSKLYAEIEKSSELLKGPVYNLDN 300

Query: 301 EKPIPQYLIGDSCFPLLPWLLTPYMELNEEDSSGFCGRAFNSTHGRAMALVNTAFCRLRA 360
           EKPIPQYLIGDSCFPLLPWLLTPYMELNEEDSSGFCGRAFNSTHGRAMALVNTAFCRLRA
Sbjct: 301 EKPIPQYLIGDSCFPLLPWLLTPYMELNEEDSSGFCGRAFNSTHGRAMALVNTAFCRLRA 360

Query: 361 RWKLLSKPWKEGCRDFFPFIILTGCLLQNFLIKCSEKLDEEQDQEEGASCSSEEQKFPLF 420
           RWKLLSKPWKEGCRDFFPFIILTGCLLQNFLIKCSEKLDEEQDQEEGASCSSEEQKFPLF
Sbjct: 361 RWKLLSKPWKEGCRDFFPFIILTGCLLQNFLIKCSEKLDEEQDQEEGASCSSEEQKFPLF 420

Query: 421 DGEIGDGRGKDIRDALALHLSSLNYRR 448
           DGEIGDGRGKDIRDALALHLSSLNYRR
Sbjct: 421 DGEIGDGRGKDIRDALALHLSSLNYRR 447

BLAST of Csa2G008700 vs. TrEMBL
Match: M5VQK6_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa005929mg PE=4 SV=1)

HSP 1 Score: 503.1 bits (1294), Expect = 3.6e-139
Identity = 262/435 (60.23%), Postives = 322/435 (74.02%), Query Frame = 1

Query: 21  AAAAITRSKAKKLDQENHL---NHQLITLIETTISSAHSFLSLNDLHLLPSQTLALESLL 80
           AA + T+ KAK   + N      H L++L+ T  S AHSFLS NDL LLPSQTL LE+LL
Sbjct: 10  AAKSTTKGKAKNKKRHNKKPLSQHHLVSLVATATSLAHSFLSQNDLLLLPSQTLTLETLL 69

Query: 81  CSTSSSLHALS--PRLPKLSLPPPLPPPRQCWFQRFLSATS-DVDCDPRWNLSFRMSKSS 140
            STS+SL  L   P  P      P PPP +CWF RFLSATS   + D RW+ +FRMS+ S
Sbjct: 70  SSTSTSLSTLLCFPNPPPQPPLRPPPPPLECWFSRFLSATSASRNFDSRWSYTFRMSEHS 129

Query: 141 FSLLLRLLSPIQSSPSSSVPPDCALAAALFRLAHGASYKAVGRRFGIDSADACRSFYAVC 200
           FS+LL LLSP  +S   S+PP+  LAAA++RLAHGASYKAVGRRFG+DS +ACR+F+AVC
Sbjct: 130 FSILLSLLSPFLNSTIPSIPPNFVLAAAIYRLAHGASYKAVGRRFGLDSVEACRAFFAVC 189

Query: 201 KAINEKLGHLLELRSDIDRIVVGFGWISLPNCCGVLGLRRFGFEGEL--KNGSLLVQALV 260
           KA+++KLG+L E RSDI RIV GFGWISLPNCCGVLG  RFG  GE+   NGSLLVQALV
Sbjct: 190 KAVSDKLGNLFEFRSDIARIVGGFGWISLPNCCGVLGFGRFGVGGEVLGPNGSLLVQALV 249

Query: 261 DAEGRFLDVSAGWPSSMKPATILRQSKLYAEIEKSSELLKGPVYNLDNEKPIPQYLIGDS 320
           D+EGRFLDVSAGWPS+MK  +I RQ+KLY  +E+S +LL GPVY L N K IPQY++GDS
Sbjct: 250 DSEGRFLDVSAGWPSAMKLESIFRQTKLYLGVEESRDLLNGPVYELGNGKAIPQYILGDS 309

Query: 321 CFPLLPWLLTPYMELNEEDSSGFCGRAFNSTHGRAMALVNTAFCRLRARWKLLSKPWKEG 380
           CFPLLPWLLTPY+  +E DS G   +AFNS H RAM LV+TAF R+RARW+LLS+ WKE 
Sbjct: 310 CFPLLPWLLTPYIRSDEADSFGSLEKAFNSVHSRAMGLVDTAFGRVRARWQLLSRQWKEE 369

Query: 381 CRDFFPFIILTGCLLQNFLIKCSEKLDEEQDQEEGASCSSEEQKFPLFDGEIGDGRGKDI 440
           C +F PF+I+TGCLL NFLIKCSE + ++  +      SS E++ P+F G++ D  G+ +
Sbjct: 370 CVEFLPFVIVTGCLLHNFLIKCSEPMPDDNVK------SSREEELPVFHGQV-DESGERM 429

Query: 441 RDALALHLSSLNYRR 448
           RD LA HLS ++ RR
Sbjct: 430 RDVLAAHLSRVSLRR 437

BLAST of Csa2G008700 vs. TrEMBL
Match: A0A068UQF2_COFCA (Uncharacterized protein OS=Coffea canephora GN=GSCOC_T00031239001 PE=4 SV=1)

HSP 1 Score: 469.5 bits (1207), Expect = 4.4e-129
Identity = 264/473 (55.81%), Postives = 320/473 (67.65%), Query Frame = 1

Query: 1   MATRGLAGDKRTTRSSAMNAAAAAITRSKAKK--------------LDQENHLNHQ---- 60
           MA    AG KR  ++          T+SK+KK              L   +  NH     
Sbjct: 1   MAAATTAGRKRRKKAK---------TKSKSKKPKTAPQPPPPPPLPLPPTSSSNHHEDLS 60

Query: 61  -LITLIETTISSAHSFLSLNDLHLLPSQTLALESLLCSTSSS---LHALSPRLPK-LSLP 120
            LI  + T   SA SFL   DLHLLPSQ+L+LESLLCSTS+S   L +L+   P+ L LP
Sbjct: 61  SLIPHLVTATYSAISFLRHQDLHLLPSQSLSLESLLCSTSTSFSKLLSLTSFFPESLPLP 120

Query: 121 PPLPPP-RQCWFQRFLSATSDVDCDPRWNLSFRMSKSSFSLLLRLLSPIQSSPSSSVPPD 180
           PPLPPP  QCWF RFL++ +  D DPRW   F +SK SF+LLLRLL+P  SS  S +PP+
Sbjct: 121 PPLPPPPAQCWFDRFLTSAA-ADYDPRWTHFFNLSKPSFTLLLRLLTPSLSS-LSPLPPN 180

Query: 181 CALAAALFRLAHGASYKAVGRRFGIDSADACRSFYAVCKAINEKLGHLLELRSDIDRIVV 240
            ALAA LFRLAH AS+ AV RRF IDS  ACR+FY VCKAINE LGHL E +SDI+RI+V
Sbjct: 181 FALAATLFRLAHSASFSAVSRRFNIDSPAACRAFYTVCKAINENLGHLFEFKSDINRIIV 240

Query: 241 GFGWISLPNCCGVLGLRRFGFEGEL--KNGSLLVQALVDAEGRFLDVSAGWPSSMKPATI 300
           GFGWISLPNCCGVLGL +F  +G+L  +NGSL+VQALVD+EGRFLDVSAGWPS++ P  +
Sbjct: 241 GFGWISLPNCCGVLGLEKFKLDGDLLGENGSLVVQALVDSEGRFLDVSAGWPSTLTPEKV 300

Query: 301 LRQSKLYAEIEKSSELLKGPVYNLDNEKPIPQYLIGDSCFPLLPWLLTPYMELNEEDSSG 360
           LRQSKL + +E++ E L GP + L +   IPQY++GDSCFPLLPWLLTPY +L+E     
Sbjct: 301 LRQSKLLSGVEETKEYLNGPSFELSDGNSIPQYILGDSCFPLLPWLLTPYKKLDENAGLN 360

Query: 361 FCGRAFNSTHGRAMALVNTAFCRLRARWKLLSKPWKEGCRDFFPFIILTGCLLQNFLIKC 420
               AFNS H   M LV  AF R+R RWKL++K W E C + FPF+I+T CLL NFLIKC
Sbjct: 361 SSEMAFNSVHSSGMELVRMAFGRVRKRWKLVAKKWSEQCVEAFPFVIVTCCLLHNFLIKC 420

Query: 421 SEKLDEEQDQEEGASCSSEEQKFPLFDGEIGDGRGKDIRDALALHLSSLNYRR 448
           SE +     Q+E A C S +Q+FP+FDGE+ D  GK IRDALA HLS  N RR
Sbjct: 421 SEAV-----QDEDAEC-SRDQEFPVFDGEV-DESGKRIRDALASHLSRANERR 455

BLAST of Csa2G008700 vs. TrEMBL
Match: A0A067KEG1_JATCU (Uncharacterized protein OS=Jatropha curcas GN=JCGZ_16986 PE=4 SV=1)

HSP 1 Score: 450.7 bits (1158), Expect = 2.1e-123
Identity = 246/450 (54.67%), Postives = 306/450 (68.00%), Query Frame = 1

Query: 1   MATRGLAGDKRTTRSSAMNAAAAAITRSKAKKLDQENHLNHQLITLIETTISSAHSFLSL 60
           MA  G  G  ++T      +   A  R   K  +Q   L+ QLI+L+    S+A+SFLS 
Sbjct: 1   MAAAG--GSSKSTAQKTSTSPKPATKRKPRKPRNQNKRLSQQLISLLSAAASAAYSFLSH 60

Query: 61  NDLHLLPSQTLALESLLCSTSSSLHALSPRLPKLSLPPPLPPPRQCWFQRFLSATSDVDC 120
           NDL LLPSQ+L++ESLL S   SL   SP L          P  Q +F RFLS+ +  D 
Sbjct: 61  NDLRLLPSQSLSIESLLSSLPFSL---SPSLSHF-------PSSQSFFHRFLSSAAS-DF 120

Query: 121 DPRWNLSFRMSKSSFSLLLRLLSP-IQSSPSSSVPPDCALAAALFRLAHGASYKAVGRRF 180
           DPRW+  FRMSK +F  LL LLSP + S    S+PPD A+AA LFRL+HGASY++  R F
Sbjct: 121 DPRWSEFFRMSKPTFCQLLSLLSPSLLSFLPPSIPPDSAIAATLFRLSHGASYESAARMF 180

Query: 181 GIDS-ADACRSFYAVCKAINEKLGHLLELRSDIDRIVVGFGWISLPNCCGVLGLRRFGFE 240
           G+DS A AC SFY+VCKA+ EKLG L++   D++ I+ GFGWISLPNCCGVLG   FG +
Sbjct: 181 GLDSSAAACLSFYSVCKAVTEKLGDLVDFGRDLEHIMAGFGWISLPNCCGVLGFGTFGVD 240

Query: 241 GEL--KNGSLLVQALVDAEGRFLDVSAGWPSSMKPATILRQSKLYAEIEKSSELLKGPVY 300
            E+  KNG+LLVQALVD+EGRFLD+SAGWP +MKP +I RQ+KLY+ IE+S ELLKGP Y
Sbjct: 241 SEILGKNGTLLVQALVDSEGRFLDISAGWPCTMKPESIFRQTKLYSRIEESRELLKGPCY 300

Query: 301 NLDNEKPIPQYLIGDSCFPLLPWLLTPYMELNEEDSSGFCGRAFNSTHGRAMALVNTAFC 360
           NL N   IPQY++GDS FPLL WLLTPY+   EEDS G   R FNS H RAM LV TAF 
Sbjct: 301 NLSNGNSIPQYILGDSSFPLLNWLLTPYIRPKEEDSFGSAQREFNSAHSRAMGLVATAFG 360

Query: 361 RLRARWKLLSKPWKEGCRDFFPFIILTGCLLQNFLIKCSEKLDEEQDQEEGASCSSEEQK 420
           R++ RW+LL++ WKE C +FFPF+I+ GCLL NFLIKCSE L      EE      +E++
Sbjct: 361 RVKKRWQLLARKWKEECVEFFPFVIVMGCLLHNFLIKCSEPL-----PEECVGGFLQEEE 420

Query: 421 FPLFDGEIGDGRGKDIRDALALHLSSLNYR 447
            P+F GE  D RG+ IRDALA+HLS ++ R
Sbjct: 421 LPVFQGE-ADERGQRIRDALAMHLSRVSIR 431

BLAST of Csa2G008700 vs. TrEMBL
Match: A0A0V0I7U5_SOLCH (Putative ovule protein OS=Solanum chacoense PE=4 SV=1)

HSP 1 Score: 442.2 bits (1136), Expect = 7.6e-121
Identity = 245/440 (55.68%), Postives = 302/440 (68.64%), Query Frame = 1

Query: 31  KKLDQENHLNHQ----LITLIETTISSAHSFLSLNDLHLLPSQTLALESLLCSTSSSLHA 90
           KK      L HQ    LI  +   ISSAHSFL  +DLHLLP Q+L+LESL+ S+SSS+  
Sbjct: 10  KKFKATLQLRHQHCTKLIPHLIAAISSAHSFLLRHDLHLLPHQSLSLESLISSSSSSISN 69

Query: 91  LSPRLPKLSLPPP----LPPPRQ-------------CWFQRFLSATSDVDCDPRWNLSFR 150
           +   +  LSLPPP     PPPR              CWFQRFL A    D D  W  +F 
Sbjct: 70  I---VSLLSLPPPPPRAAPPPRAAVTSPSDDDSLPGCWFQRFLIA----DSDTLWAETFN 129

Query: 151 MSKSSFSLLLRLLSPIQSSPSSSVPPDCALAAALFRLAHGASYKAVGRRFGIDSADACRS 210
           +++ SF+LLLRLL+P  S  S SVPP+ ALA  L+RLAHGAS+ AV RRFGIDS  ACR 
Sbjct: 130 LTEPSFTLLLRLLTP--SLSSLSVPPNYALALTLYRLAHGASFSAVSRRFGIDSPTACRV 189

Query: 211 FYAVCKAINEKLGHLLELRSDIDRIVVGFGWISLPNCCGVLGLRRFGFEGEL--KNGSLL 270
           FY VCKAI E LGHL ELRSDI+R++VGFGWISLPNCCGVLG+ +F   G+L  +NG L+
Sbjct: 190 FYTVCKAITENLGHLFELRSDINRVIVGFGWISLPNCCGVLGIEKFELGGDLLGENGFLI 249

Query: 271 VQALVDAEGRFLDVSAGWPSSMKPATILRQSKLYAEIEKSSELLKGPVYNLDNEKPIPQY 330
           VQALVD+EGRFLDVSAGWPS+M+P T+LR+SKLY  +E+S E L G  + L++   IPQY
Sbjct: 250 VQALVDSEGRFLDVSAGWPSTMRPETVLRKSKLYLGVEESKEYLNGSSFELNDGNSIPQY 309

Query: 331 LIGDSCFPLLPWLLTPYM-ELNEEDSSGFCGRAFNSTHGRAMALVNTAFCRLRARWKLLS 390
           ++GDSCFPLLPW+LTPY  E N ED +     AFNS H R M LV TAF R+R +WKLL+
Sbjct: 310 ILGDSCFPLLPWVLTPYRGESNLEDGAEM---AFNSVHRRGMQLVGTAFGRVREKWKLLA 369

Query: 391 KPWKEGCRDFFPFIILTGCLLQNFLIKCSEKLDEEQDQEEGASCSSEEQKFPLFDGEIGD 447
           + W E C + FPF+I+T CLL NFLIKCSE + +E ++E         ++FP+FDGE+ D
Sbjct: 370 RKWNEQCIEAFPFVIVTCCLLHNFLIKCSEAVTDETEEE-----YPRFEEFPVFDGEV-D 429

BLAST of Csa2G008700 vs. TAIR10
Match: AT1G72270.1 (AT1G72270.1 Ribosome 60S biogenesis N-terminal (InterPro:IPR021714))

HSP 1 Score: 278.5 bits (711), Expect = 7.3e-75
Identity = 178/415 (42.89%), Postives = 234/415 (56.39%), Query Frame = 1

Query: 39  LNHQLITLIETTISSAHSFLSLNDLHLLPSQTLALESLLCSTSSSLHALSPRLPKLSLPP 98
           L   L+  + +  +  +SFL  NDL L PSQTL LESL+ S   S    SP     ++  
Sbjct: 21  LKDPLLRRLSSAAAVTNSFLQANDLFLSPSQTLRLESLISSLPISP---SPSSSSSAITT 80

Query: 99  PLPPPRQCWFQRFLSATSDVDCDPRWNLSFRMSKSSFSLLLRLLSPIQSSPSSSVPPDCA 158
                   WF RFL++ ++ + DPRW L FRMSKS+F  L  +LS       SS+P   +
Sbjct: 81  TT------WFNRFLTSATEDEDDPRWCLYFRMSKSTFFSLYSILS------HSSLP---S 140

Query: 159 LAAALFRLAHGASYKAVGRRFGIDS-ADACRSFYAVCKAINEKLGHLLELRSDIDRIVVG 218
            AA +FRLAHGASY+ +  RFG DS + A RSF+ VCK INEKL   L      D     
Sbjct: 141 FAATIFRLAHGASYECLVHRFGFDSTSQASRSFFTVCKLINEKLSQQL------DDPKPD 200

Query: 219 FGWISLPNCCGVLGLRRFGFEGEL--KNGSLLVQALVDAEGRFLDVSAGWPSSMKPATIL 278
           F    LPNC GV+G  RF  +G+L    GS+LVQALVD+ GRF+D+SAGWPS+MKP  I 
Sbjct: 201 FSPNLLPNCYGVVGFGRFEVKGKLLGAKGSILVQALVDSNGRFVDISAGWPSTMKPEAIF 260

Query: 279 RQSKLYAEIEKSSELLKGPVYNLDNEKPIPQYLIGDSCFPLLPWLLTPYMELNEEDSSGF 338
           RQ+KL++  E   E+L G    L N   +P+Y++GDSC PLLPWL+TPY   ++E+S   
Sbjct: 261 RQTKLFSIAE---EVLSGAPTKLGNGVLVPRYILGDSCLPLLPWLVTPYDLTSDEES--- 320

Query: 339 CGRAFNSTHGRAMALVNTAFCRLRARWKLLSKPWKEGCRDFFPFIILTGCLLQNFLIKCS 398
               FN+     +  V  AF ++RARW++L K WK    +F PF+I TGCLL NFL+   
Sbjct: 321 FREEFNNVVHTGLHSVEIAFAKVRARWRILDKKWKPETIEFMPFVITTGCLLHNFLVNSG 380

Query: 399 EKLD---------EEQDQEEGASCSSEEQKFPLFDGEIGDGRGKDIRDALALHLS 442
           +  D         E  D  E      +E++   F+GE      K IRDA+A +LS
Sbjct: 381 DDDDSVEECVNGCEAGDNGEMRKDDDKEEETRSFEGE-AYRESKRIRDAIAENLS 404

BLAST of Csa2G008700 vs. TAIR10
Match: AT3G55350.1 (AT3G55350.1 PIF / Ping-Pong family of plant transposases)

HSP 1 Score: 139.8 bits (351), Expect = 4.1e-33
Identity = 104/361 (28.81%), Postives = 159/361 (44.04%), Query Frame = 1

Query: 107 WFQRFLSATSDVDCDPR-WNLSFRMSKSSFSLLLRLLS------PIQSSPSSSVPPDC-- 166
           W+  F         DP+ +   F++S+ +F  +  L+       P   S S+  P     
Sbjct: 54  WWDGFSRRIYGGSTDPKTFESVFKISRKTFDYICSLVKADFTAKPANFSDSNGNPLSLND 113

Query: 167 ALAAALFRLAHGASYKAVGRRFGIDSADACRSFYAVCKAINEKLGHLLELRSDIDRIVVG 226
            +A AL RL  G S   +G  FG++ +   +  +   +++ E+  H L   S +D I   
Sbjct: 114 RVAVALRRLGSGESLSVIGETFGMNQSTVSQITWRFVESMEERAIHHLSWPSKLDEIKSK 173

Query: 227 FGWIS-LPNCCGVLGL-------------RRFGFEGELKNGSLLVQALVDAEGRFLDVSA 286
           F  IS LPNCCG + +              +   +GE KN S+ +QA+VD + RFLDV A
Sbjct: 174 FEKISGLPNCCGAIDITHIVMNLPAVEPSNKVWLDGE-KNFSMTLQAVVDPDMRFLDVIA 233

Query: 287 GWPSSMKPATILRQSKLYAEIEKSSELLKGPVYNLDNEKPIPQYLIGDSCFPLLPWLLTP 346
           GWP S+    +L+ S  Y  +EK    L G    L     + +Y++GDS FPLLPWLLTP
Sbjct: 234 GWPGSLNDDVVLKNSGFYKLVEKGKR-LNGEKLPLSERTELREYIVGDSGFPLLPWLLTP 293

Query: 347 YMELNEEDSSGFCGRAFNSTHGRAMALVNTAFCRLRARWKLLSKPWKEGCRDFFPFIILT 406
           Y    +   +      FN  H  A      A  +L+ RW++++       R+  P II  
Sbjct: 294 Y----QGKPTSLPQTEFNKRHSEATKAAQMALSKLKDRWRIINGVMWMPDRNRLPRIIFV 353

Query: 407 GCLLQNFLIKCSEKLDEEQDQEEGASCSSEEQKFPLFDGEIGDGRGKDIRDALALHLSSL 445
            CLL N +I       E+Q  ++       +  +     ++ D     +RD L+  L   
Sbjct: 354 CCLLHNIIIDM-----EDQTLDDQPLSQQHDMNYRQRSCKLADEASSVLRDELSDQLCGK 403

BLAST of Csa2G008700 vs. TAIR10
Match: AT3G63270.1 (AT3G63270.1 Putative harbinger transposase-derived nuclease (InterPro:IPR006912))

HSP 1 Score: 125.9 bits (315), Expect = 6.1e-29
Identity = 93/297 (31.31%), Postives = 140/297 (47.14%), Query Frame = 1

Query: 128 FRMSKSSFSLLLRLLSP--IQSSPSSSVPPDCAL-------AAALFRLAHGASYKAVGRR 187
           FR SK++FS +  L+    I   PS  +  +  L       A AL RLA G S  +VG  
Sbjct: 69  FRASKTTFSYICSLVREDLISRPPSGLINIEGRLLSVEKQVAIALRRLASGDSQVSVGAA 128

Query: 188 FGIDSADACRSFYAVCKAINEKLGHLLELRSD--IDRIVVGFGWI-SLPNCCGVLGLRRF 247
           FG+  +   +  +   +A+ E+  H L       I+ I   F  +  LPNCCG +     
Sbjct: 129 FGVGQSTVSQVTWRFIEALEERAKHHLRWPDSDRIEEIKSKFEEMYGLPNCCGAIDTTHI 188

Query: 248 -----------GFEGELKNGSLLVQALVDAEGRFLDVSAGWPSSMKPATILRQSKLYAEI 307
                       +  + KN S+ +Q + D E RFL++  GWP  M  + +L+ S  +   
Sbjct: 189 IMTLPAVQASDDWCDQEKNYSMFLQGVFDHEMRFLNMVTGWPGGMTVSKLLKFSGFFKLC 248

Query: 308 EKSSELLKGPVYNLDNEKPIPQYLIGDSCFPLLPWLLTPYMELNEEDSSGFCGRAFNSTH 367
           E ++++L G    L     I +Y++G   +PLLPWL+TP+   +  DS      AFN  H
Sbjct: 249 E-NAQILDGNPKTLSQGAQIREYVVGGISYPLLPWLITPHDSDHPSDSMV----AFNERH 308

Query: 368 GRAMALVNTAFCRLRARWKLLSKPWKEGCRDFFPFIILTGCLLQNFLIKCSEKLDEE 402
            +  ++  TAF +L+  W++LSK      R   P IIL  CLL N +I C + L E+
Sbjct: 309 EKVRSVAATAFQQLKGSWRILSKVMWRPDRRKLPSIILVCCLLHNIIIDCGDYLQED 360

BLAST of Csa2G008700 vs. TAIR10
Match: AT5G12010.1 (AT5G12010.1 unknown protein)

HSP 1 Score: 90.1 bits (222), Expect = 3.7e-18
Identity = 78/300 (26.00%), Postives = 123/300 (41.00%), Query Frame = 1

Query: 127 SFRMSKSSFSLLLRLLSPIQSSPSSS----VPPDCALAAALFRLAHGASYKAVGRRFGID 186
           +FRMSKS+F L+   L+   +   ++    +P    +A  ++RLA G   + V ++FG+ 
Sbjct: 178 AFRMSKSTFELICDELNSAVAKEDTALRNAIPVRQRVAVCIWRLATGEPLRLVSKKFGLG 237

Query: 187 SADACRSFYAVCKAINEKL----------------GHLLELRSDIDRIVVGFGWISLPNC 246
            +   +    VCKAI + L                    E  S I  +V       +P  
Sbjct: 238 ISTCHKLVLEVCKAIKDVLMPKYLQWPDDESLRNIRERFESVSGIPNVVGSMYTTHIPII 297

Query: 247 CGVLGL-----RRFGFEGELKNGSLLVQALVDAEGRFLDVSAGWPSSMKPATILRQSKLY 306
              + +     +R     +  + S+ +QA+V+ +G F D+  GWP SM    +L +S LY
Sbjct: 298 APKISVASYFNKRHTERNQKTSYSITIQAVVNPKGVFTDLCIGWPGSMPDDKVLEKSLLY 357

Query: 307 AEIEKSSELLKGPVYNLDNEKPIPQYLIGDSCFPLLPWLLTPYMELNEEDSSGFCGRAFN 366
                   LLKG             ++ G    PLL W+L PY + N      +   AFN
Sbjct: 358 QRANNGG-LLKG------------MWVAGGPGHPLLDWVLVPYTQQN----LTWTQHAFN 417

Query: 367 STHGRAMALVNTAFCRLRARWKLLSKPWKEGCRDFFPFIILTGCLLQNFLIKCSEKLDEE 402
                   +   AF RL+ RW  L K  +   +D  P ++   C+L N      EK++ E
Sbjct: 418 EKMSEVQGVAKEAFGRLKGRWACLQKRTEVKLQD-LPTVLGACCVLHNICEMREEKMEPE 459

BLAST of Csa2G008700 vs. TAIR10
Match: AT3G19120.1 (AT3G19120.1 PIF / Ping-Pong family of plant transposases)

HSP 1 Score: 85.1 bits (209), Expect = 1.2e-16
Identity = 110/419 (26.25%), Postives = 182/419 (43.44%), Query Frame = 1

Query: 56  SFLSLNDLHLLPSQTLA--LESLLCSTSSSLHALSPRLPKLSLPPPLPPPRQCWFQRFLS 115
           S LS +    L   TLA  L  L  + SS+  + S   P  S PPPL          F +
Sbjct: 41  SLLSTSSAAPLLFFTLASLLSFLAVNRSSTESSSSSESPSPSPPPPLADGDYS-VAAFRA 100

Query: 116 ATSD----VDC---DPRWNLSFRMSKSSFSLLLRLLSPIQSSPSSSVPPDCALAAALFRL 175
            T+D    +D    D RW   + +S   F  ++  L P  ++ + S+P D A+A  L RL
Sbjct: 101 LTTDHIWSLDAPLRDARWRSLYGLSYPVFITVVDKLKPFITASNLSLPADYAVAMVLSRL 160

Query: 176 AHGASYKAVGRRFGIDSADACRSFYAVCKAINEKL-GHLLELRSDIDRIV---VGFGWI- 235
           AHG S K +  R+ +D     +    V + +  KL    +++     R++    GF  + 
Sbjct: 161 AHGCSAKTLASRYSLDPYLISKITNMVTRLLATKLYPEFIKIPVGKRRLIETTQGFEELT 220

Query: 236 SLPNCCGVLG-----LRR-------------FGFEGELKNGSLLVQALVDAEGRFLDVSA 295
           SLPN CG +      LRR             +G++      ++L+Q + D +  F DV  
Sbjct: 221 SLPNICGAIDSTPVKLRRRTKLNPRNIYGCKYGYD------AVLLQVVADHKKIFWDVCV 280

Query: 296 GWPSSMKPATILRQSKLYAEIEKSSELLKGPVYNLDNEKPIPQYLIGDSCFPLLPWLLTP 355
             P     ++  R S LY  +  S +++   V N+      P Y++GD C+PLL +L+TP
Sbjct: 281 KAPGGEDDSSHFRDSLLYKRL-TSGDIVWEKVINIRGHHVRP-YIVGDWCYPLLSFLMTP 340

Query: 356 YMELNEEDSSGFCGRAFNSTHGRAMALVNTAFCRLRARWKLLSKPWKEGCRDFFPFIILT 415
           +   +   S       F+    +  ++V  A   L+ARWK+L +    G  +  P  I+ 
Sbjct: 341 F---SPNGSGTPPENLFDGMLMKGRSVVVEAIGLLKARWKIL-QSLNVGV-NHAPQTIVA 400

Query: 416 GCLLQNFLIKCSEKLDEE--QDQEEG---ASCSSEEQKFPLFDGEIGDGRGKDIRDALA 438
            C+L N L + + + + E  +D +E    A     E++F  +   +     +D+   L+
Sbjct: 401 CCVLHN-LCQIAREPEPEIWKDPDEAGTPARVLESERQFYYYGESLRQALAEDLHQRLS 444

BLAST of Csa2G008700 vs. NCBI nr
Match: gi|449443271|ref|XP_004139403.1| (PREDICTED: putative nuclease HARBI1 [Cucumis sativus])

HSP 1 Score: 902.5 bits (2331), Expect = 2.9e-259
Identity = 447/447 (100.00%), Postives = 447/447 (100.00%), Query Frame = 1

Query: 1   MATRGLAGDKRTTRSSAMNAAAAAITRSKAKKLDQENHLNHQLITLIETTISSAHSFLSL 60
           MATRGLAGDKRTTRSSAMNAAAAAITRSKAKKLDQENHLNHQLITLIETTISSAHSFLSL
Sbjct: 1   MATRGLAGDKRTTRSSAMNAAAAAITRSKAKKLDQENHLNHQLITLIETTISSAHSFLSL 60

Query: 61  NDLHLLPSQTLALESLLCSTSSSLHALSPRLPKLSLPPPLPPPRQCWFQRFLSATSDVDC 120
           NDLHLLPSQTLALESLLCSTSSSLHALSPRLPKLSLPPPLPPPRQCWFQRFLSATSDVDC
Sbjct: 61  NDLHLLPSQTLALESLLCSTSSSLHALSPRLPKLSLPPPLPPPRQCWFQRFLSATSDVDC 120

Query: 121 DPRWNLSFRMSKSSFSLLLRLLSPIQSSPSSSVPPDCALAAALFRLAHGASYKAVGRRFG 180
           DPRWNLSFRMSKSSFSLLLRLLSPIQSSPSSSVPPDCALAAALFRLAHGASYKAVGRRFG
Sbjct: 121 DPRWNLSFRMSKSSFSLLLRLLSPIQSSPSSSVPPDCALAAALFRLAHGASYKAVGRRFG 180

Query: 181 IDSADACRSFYAVCKAINEKLGHLLELRSDIDRIVVGFGWISLPNCCGVLGLRRFGFEGE 240
           IDSADACRSFYAVCKAINEKLGHLLELRSDIDRIVVGFGWISLPNCCGVLGLRRFGFEGE
Sbjct: 181 IDSADACRSFYAVCKAINEKLGHLLELRSDIDRIVVGFGWISLPNCCGVLGLRRFGFEGE 240

Query: 241 LKNGSLLVQALVDAEGRFLDVSAGWPSSMKPATILRQSKLYAEIEKSSELLKGPVYNLDN 300
           LKNGSLLVQALVDAEGRFLDVSAGWPSSMKPATILRQSKLYAEIEKSSELLKGPVYNLDN
Sbjct: 241 LKNGSLLVQALVDAEGRFLDVSAGWPSSMKPATILRQSKLYAEIEKSSELLKGPVYNLDN 300

Query: 301 EKPIPQYLIGDSCFPLLPWLLTPYMELNEEDSSGFCGRAFNSTHGRAMALVNTAFCRLRA 360
           EKPIPQYLIGDSCFPLLPWLLTPYMELNEEDSSGFCGRAFNSTHGRAMALVNTAFCRLRA
Sbjct: 301 EKPIPQYLIGDSCFPLLPWLLTPYMELNEEDSSGFCGRAFNSTHGRAMALVNTAFCRLRA 360

Query: 361 RWKLLSKPWKEGCRDFFPFIILTGCLLQNFLIKCSEKLDEEQDQEEGASCSSEEQKFPLF 420
           RWKLLSKPWKEGCRDFFPFIILTGCLLQNFLIKCSEKLDEEQDQEEGASCSSEEQKFPLF
Sbjct: 361 RWKLLSKPWKEGCRDFFPFIILTGCLLQNFLIKCSEKLDEEQDQEEGASCSSEEQKFPLF 420

Query: 421 DGEIGDGRGKDIRDALALHLSSLNYRR 448
           DGEIGDGRGKDIRDALALHLSSLNYRR
Sbjct: 421 DGEIGDGRGKDIRDALALHLSSLNYRR 447

BLAST of Csa2G008700 vs. NCBI nr
Match: gi|659070933|ref|XP_008457314.1| (PREDICTED: putative nuclease HARBI1 [Cucumis melo])

HSP 1 Score: 865.9 bits (2236), Expect = 3.0e-248
Identity = 434/447 (97.09%), Postives = 437/447 (97.76%), Query Frame = 1

Query: 1   MATRGLAGDKRTTRSSAMNAAAAAITRSKAKKLDQENHLNHQLITLIETTISSAHSFLSL 60
           MATRGLAGDKRTTRSSAMN AAAAITRSKAKKLDQENHLNHQLITLIETTISSA SFLSL
Sbjct: 1   MATRGLAGDKRTTRSSAMN-AAAAITRSKAKKLDQENHLNHQLITLIETTISSARSFLSL 60

Query: 61  NDLHLLPSQTLALESLLCSTSSSLHALSPRLPKLSLPPPLPPPRQCWFQRFLSATSDVDC 120
           NDLHLLPSQTLALESLLCSTSSSLHALSPRLPKLSLP PLPPPRQCWFQRFLSATSDVDC
Sbjct: 61  NDLHLLPSQTLALESLLCSTSSSLHALSPRLPKLSLPSPLPPPRQCWFQRFLSATSDVDC 120

Query: 121 DPRWNLSFRMSKSSFSLLLRLLSPIQSSPSSSVPPDCALAAALFRLAHGASYKAVGRRFG 180
           DPRWNLSFRMSKSSFSLLLRLLSPIQS  SSSVPPDCALAAALFRLAHGASYKAVGRRFG
Sbjct: 121 DPRWNLSFRMSKSSFSLLLRLLSPIQSPSSSSVPPDCALAAALFRLAHGASYKAVGRRFG 180

Query: 181 IDSADACRSFYAVCKAINEKLGHLLELRSDIDRIVVGFGWISLPNCCGVLGLRRFGFEGE 240
           IDSADACRSFYAVCKAINEKLGHLLELRSDIDRIVVGFGWISLPNCCGVLGLRRFGFEGE
Sbjct: 181 IDSADACRSFYAVCKAINEKLGHLLELRSDIDRIVVGFGWISLPNCCGVLGLRRFGFEGE 240

Query: 241 LKNGSLLVQALVDAEGRFLDVSAGWPSSMKPATILRQSKLYAEIEKSSELLKGPVYNLDN 300
           LKNGSLLVQALVDAEGRFLDVSAGWPSSMKPATILRQSKLY EIEKSSELLKGPVYNLD+
Sbjct: 241 LKNGSLLVQALVDAEGRFLDVSAGWPSSMKPATILRQSKLYEEIEKSSELLKGPVYNLDD 300

Query: 301 EKPIPQYLIGDSCFPLLPWLLTPYMELNEEDSSGFCGRAFNSTHGRAMALVNTAFCRLRA 360
           EKPIPQYLIGDSCFPL PWLLTPY+ELNEEDSSGF  RAFNSTHGRAMALVNTAFCRLRA
Sbjct: 301 EKPIPQYLIGDSCFPLFPWLLTPYIELNEEDSSGFRERAFNSTHGRAMALVNTAFCRLRA 360

Query: 361 RWKLLSKPWKEGCRDFFPFIILTGCLLQNFLIKCSEKLDEEQDQEEGASCSSEEQKFPLF 420
           RWKLLSKPWKEGCRDFFPFIILTGCLLQNFLIKCSEKLDEEQDQEEGASCSSEEQKFP F
Sbjct: 361 RWKLLSKPWKEGCRDFFPFIILTGCLLQNFLIKCSEKLDEEQDQEEGASCSSEEQKFPPF 420

Query: 421 DGEIGDGRGKDIRDALALHLSSLNYRR 448
           DGEIGDGRGKDIRDALALHLSSL+YRR
Sbjct: 421 DGEIGDGRGKDIRDALALHLSSLSYRR 446

BLAST of Csa2G008700 vs. NCBI nr
Match: gi|595803820|ref|XP_007202067.1| (hypothetical protein PRUPE_ppa005929mg [Prunus persica])

HSP 1 Score: 503.1 bits (1294), Expect = 5.2e-139
Identity = 262/435 (60.23%), Postives = 322/435 (74.02%), Query Frame = 1

Query: 21  AAAAITRSKAKKLDQENHL---NHQLITLIETTISSAHSFLSLNDLHLLPSQTLALESLL 80
           AA + T+ KAK   + N      H L++L+ T  S AHSFLS NDL LLPSQTL LE+LL
Sbjct: 10  AAKSTTKGKAKNKKRHNKKPLSQHHLVSLVATATSLAHSFLSQNDLLLLPSQTLTLETLL 69

Query: 81  CSTSSSLHALS--PRLPKLSLPPPLPPPRQCWFQRFLSATS-DVDCDPRWNLSFRMSKSS 140
            STS+SL  L   P  P      P PPP +CWF RFLSATS   + D RW+ +FRMS+ S
Sbjct: 70  SSTSTSLSTLLCFPNPPPQPPLRPPPPPLECWFSRFLSATSASRNFDSRWSYTFRMSEHS 129

Query: 141 FSLLLRLLSPIQSSPSSSVPPDCALAAALFRLAHGASYKAVGRRFGIDSADACRSFYAVC 200
           FS+LL LLSP  +S   S+PP+  LAAA++RLAHGASYKAVGRRFG+DS +ACR+F+AVC
Sbjct: 130 FSILLSLLSPFLNSTIPSIPPNFVLAAAIYRLAHGASYKAVGRRFGLDSVEACRAFFAVC 189

Query: 201 KAINEKLGHLLELRSDIDRIVVGFGWISLPNCCGVLGLRRFGFEGEL--KNGSLLVQALV 260
           KA+++KLG+L E RSDI RIV GFGWISLPNCCGVLG  RFG  GE+   NGSLLVQALV
Sbjct: 190 KAVSDKLGNLFEFRSDIARIVGGFGWISLPNCCGVLGFGRFGVGGEVLGPNGSLLVQALV 249

Query: 261 DAEGRFLDVSAGWPSSMKPATILRQSKLYAEIEKSSELLKGPVYNLDNEKPIPQYLIGDS 320
           D+EGRFLDVSAGWPS+MK  +I RQ+KLY  +E+S +LL GPVY L N K IPQY++GDS
Sbjct: 250 DSEGRFLDVSAGWPSAMKLESIFRQTKLYLGVEESRDLLNGPVYELGNGKAIPQYILGDS 309

Query: 321 CFPLLPWLLTPYMELNEEDSSGFCGRAFNSTHGRAMALVNTAFCRLRARWKLLSKPWKEG 380
           CFPLLPWLLTPY+  +E DS G   +AFNS H RAM LV+TAF R+RARW+LLS+ WKE 
Sbjct: 310 CFPLLPWLLTPYIRSDEADSFGSLEKAFNSVHSRAMGLVDTAFGRVRARWQLLSRQWKEE 369

Query: 381 CRDFFPFIILTGCLLQNFLIKCSEKLDEEQDQEEGASCSSEEQKFPLFDGEIGDGRGKDI 440
           C +F PF+I+TGCLL NFLIKCSE + ++  +      SS E++ P+F G++ D  G+ +
Sbjct: 370 CVEFLPFVIVTGCLLHNFLIKCSEPMPDDNVK------SSREEELPVFHGQV-DESGERM 429

Query: 441 RDALALHLSSLNYRR 448
           RD LA HLS ++ RR
Sbjct: 430 RDVLAAHLSRVSLRR 437

BLAST of Csa2G008700 vs. NCBI nr
Match: gi|645275650|ref|XP_008242923.1| (PREDICTED: putative nuclease HARBI1 [Prunus mume])

HSP 1 Score: 500.0 bits (1286), Expect = 4.4e-138
Identity = 261/435 (60.00%), Postives = 321/435 (73.79%), Query Frame = 1

Query: 21  AAAAITRSKAKKLDQENHL---NHQLITLIETTISSAHSFLSLNDLHLLPSQTLALESLL 80
           AA + T+ KAK   + N      H L++L+ T  S AHSFLS NDL LLPSQTL LE+LL
Sbjct: 10  AAKSTTKGKAKNKKRHNKKPLSQHHLVSLVATATSLAHSFLSQNDLLLLPSQTLTLETLL 69

Query: 81  CSTSSSLHALS--PRLPKLSLPPPLPPPRQCWFQRFLSATS-DVDCDPRWNLSFRMSKSS 140
            STS+SL  L   P  P      P PPP +CWF RFLSATS   + D RW+ +FRMS+ S
Sbjct: 70  SSTSTSLSTLLCFPNPPPQPPLRPPPPPLECWFSRFLSATSASRNFDSRWSHTFRMSEHS 129

Query: 141 FSLLLRLLSPIQSSPSSSVPPDCALAAALFRLAHGASYKAVGRRFGIDSADACRSFYAVC 200
           FS+LL LLSP  +S   S+PP+  LAAA++RLAHGASYKAVGRRFG+DS +ACR+F+AVC
Sbjct: 130 FSILLSLLSPFLNSTIPSIPPNFVLAAAIYRLAHGASYKAVGRRFGLDSVEACRAFFAVC 189

Query: 201 KAINEKLGHLLELRSDIDRIVVGFGWISLPNCCGVLGLRRFGFEGEL--KNGSLLVQALV 260
           KA+++KLG+L E RSDI RIV GFGWISLPNCCGVLG  RFG  GE+   NGSLLVQALV
Sbjct: 190 KAVSDKLGNLFEFRSDIARIVGGFGWISLPNCCGVLGFGRFGVGGEVLGPNGSLLVQALV 249

Query: 261 DAEGRFLDVSAGWPSSMKPATILRQSKLYAEIEKSSELLKGPVYNLDNEKPIPQYLIGDS 320
           D+EGRFLDVSAGWPS+MK  +I RQ+KLY  +E+S +LL GPVY L N K IPQY++GDS
Sbjct: 250 DSEGRFLDVSAGWPSAMKLESIFRQTKLYLGVEESRDLLNGPVYELGNGKAIPQYILGDS 309

Query: 321 CFPLLPWLLTPYMELNEEDSSGFCGRAFNSTHGRAMALVNTAFCRLRARWKLLSKPWKEG 380
           CFPLLPWLLTPY+  +E DS G   +AFNS H RAM LV+TAF R+RARW+LLS+ WKE 
Sbjct: 310 CFPLLPWLLTPYIRSDEADSFGSLEKAFNSVHSRAMGLVDTAFGRVRARWQLLSRQWKEE 369

Query: 381 CRDFFPFIILTGCLLQNFLIKCSEKLDEEQDQEEGASCSSEEQKFPLFDGEIGDGRGKDI 440
           C +F PF+I+TGCLL NFLIKCSE + ++  +      S  E++ P+F G++ D  G+ +
Sbjct: 370 CVEFLPFVIVTGCLLHNFLIKCSEPMPDDNIK------SLREEELPVFHGQV-DESGERM 429

Query: 441 RDALALHLSSLNYRR 448
           RD LA HLS ++ RR
Sbjct: 430 RDVLAAHLSRVSLRR 437

BLAST of Csa2G008700 vs. NCBI nr
Match: gi|694373640|ref|XP_009363921.1| (PREDICTED: putative nuclease HARBI1 [Pyrus x bretschneideri])

HSP 1 Score: 490.7 bits (1262), Expect = 2.7e-135
Identity = 255/443 (57.56%), Postives = 320/443 (72.23%), Query Frame = 1

Query: 12  TTRSSAMNAAAAAITRSKAKKLDQENHLNHQLITLIETTISSAHSFLSLNDLHLLPSQTL 71
           TT+ +A N   +    +  K L+Q     H L+ L+    S AHSFL  NDL LLP+QTL
Sbjct: 14  TTKGNARNNKQSQTHSNNKKALNQ-----HHLVGLVTAATSLAHSFLFQNDLLLLPAQTL 73

Query: 72  ALESLLCSTSSSLHAL----SPRLPKLSLPPPLPPPRQCWFQRFLSATS-DVDCDPRWNL 131
            LESLL S S+SL  L    +PR P+  L PP PPP +CWF RFLSAT+ D D D RW+ 
Sbjct: 74  TLESLLSSASTSLSTLLCFPNPR-PQSPLRPP-PPPLECWFSRFLSATAADGDFDFRWSQ 133

Query: 132 SFRMSKSSFSLLLRLLSPIQSSPSSSVPPDCALAAALFRLAHGASYKAVGRRFGIDSADA 191
            FRMS+ SFS+LL LLSP  +S   S+PP+  LAAA++RLAHGASYKAVGRRFG+DS DA
Sbjct: 134 IFRMSEHSFSVLLSLLSPFLNSTIPSIPPNFVLAAAIYRLAHGASYKAVGRRFGLDSMDA 193

Query: 192 CRSFYAVCKAINEKLGHLLELRSDIDRIVVGFGWISLPNCCGVLGLRRFGFEGEL--KNG 251
           CR+FY+VCKA+N++LG+L E RSDI R++  FGWISLPNCCGVLG  RF   GEL   NG
Sbjct: 194 CRAFYSVCKAVNDQLGNLFEFRSDISRVLAAFGWISLPNCCGVLGFTRFEVGGELLGANG 253

Query: 252 SLLVQALVDAEGRFLDVSAGWPSSMKPATILRQSKLYAEIEKSSELLKGPVYNLDNEKPI 311
           SLLVQA+VD+EGRFLDVSAGWPS+MKP +I RQSKLY  +E+S ELL GP + L N   I
Sbjct: 254 SLLVQAVVDSEGRFLDVSAGWPSTMKPESIFRQSKLYLGVEESRELLSGPAFELGNGNSI 313

Query: 312 PQYLIGDSCFPLLPWLLTPYMELNEEDSSGFCGRAFNSTHGRAMALVNTAFCRLRARWKL 371
           PQY++GDSCFPLLPWLLTPY+  +E DS G   +AFN+ H RAM LV TAF R+RARW+L
Sbjct: 314 PQYILGDSCFPLLPWLLTPYVRSDEADSFGSMEKAFNAVHYRAMGLVGTAFGRVRARWQL 373

Query: 372 LSKPWKEGCRDFFPFIILTGCLLQNFLIKCSEKLDEEQDQEEGASCSSEEQKFPLFDGEI 431
           L++ WKE C +F PF+++TGCLL NFLIKCSE +  +  +        +E + P+FDG++
Sbjct: 374 LARQWKEECAEFLPFVVVTGCLLHNFLIKCSEPMPNDNVR------GLKEDELPVFDGQV 433

Query: 432 GDGRGKDIRDALALHLSSLNYRR 448
            +  G+ +RD LA+HLS ++ RR
Sbjct: 434 NES-GERMRDVLAMHLSRVSLRR 442

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
HARB1_RAT8.6e-0924.19Putative nuclease HARBI1 OS=Rattus norvegicus GN=Harbi1 PE=2 SV=1[more]
HARB1_MOUSE5.6e-0824.00Putative nuclease HARBI1 OS=Mus musculus GN=Harbi1 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0LFB5_CUCSA2.0e-259100.00Uncharacterized protein OS=Cucumis sativus GN=Csa_2G008700 PE=4 SV=1[more]
M5VQK6_PRUPE3.6e-13960.23Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa005929mg PE=4 SV=1[more]
A0A068UQF2_COFCA4.4e-12955.81Uncharacterized protein OS=Coffea canephora GN=GSCOC_T00031239001 PE=4 SV=1[more]
A0A067KEG1_JATCU2.1e-12354.67Uncharacterized protein OS=Jatropha curcas GN=JCGZ_16986 PE=4 SV=1[more]
A0A0V0I7U5_SOLCH7.6e-12155.68Putative ovule protein OS=Solanum chacoense PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT1G72270.17.3e-7542.89 Ribosome 60S biogenesis N-terminal (InterPro:IPR021714)[more]
AT3G55350.14.1e-3328.81 PIF / Ping-Pong family of plant transposases[more]
AT3G63270.16.1e-2931.31 Putative harbinger transposase-derived nuclease (InterPro:IPR006912)[more]
AT5G12010.13.7e-1826.00 unknown protein[more]
AT3G19120.11.2e-1626.25 PIF / Ping-Pong family of plant transposases[more]
Match NameE-valueIdentityDescription
gi|449443271|ref|XP_004139403.1|2.9e-259100.00PREDICTED: putative nuclease HARBI1 [Cucumis sativus][more]
gi|659070933|ref|XP_008457314.1|3.0e-24897.09PREDICTED: putative nuclease HARBI1 [Cucumis melo][more]
gi|595803820|ref|XP_007202067.1|5.2e-13960.23hypothetical protein PRUPE_ppa005929mg [Prunus persica][more]
gi|645275650|ref|XP_008242923.1|4.4e-13860.00PREDICTED: putative nuclease HARBI1 [Prunus mume][more]
gi|694373640|ref|XP_009363921.1|2.7e-13557.56PREDICTED: putative nuclease HARBI1 [Pyrus x bretschneideri][more]
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR027806HARBI1_dom
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008150 biological_process
cellular_component GO:0005575 cellular_component
molecular_function GO:0003674 molecular_function
This gene is associated with the following unigenes:
Unigene NameAnalysis NameSequence type in Unigene
CU087537cucumber EST collection version 3.0transcribed_cluster
CU130187cucumber EST collection version 3.0transcribed_cluster
CU131258cucumber EST collection version 3.0transcribed_cluster
CU172772cucumber EST collection version 3.0transcribed_cluster

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Csa2G008700.1Csa2G008700.1mRNA


The following transcribed_cluster feature(s) are associated with this gene:

Feature NameUnique NameType
CU130187CU130187transcribed_cluster
CU087537CU087537transcribed_cluster
CU172772CU172772transcribed_cluster
CU131258CU131258transcribed_cluster


Analysis Name: InterPro Annotations of cucumber (Chinese Long)
Date Performed: 2016-09-28
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR027806Harbinger transposase-derived nuclease domainPFAMPF13359DDE_Tnp_4coord: 241..389
score: 6.9
NoneNo IPR availablePANTHERPTHR22930UNCHARACTERIZEDcoord: 40..440
score: 1.4
NoneNo IPR availablePANTHERPTHR22930:SF59SUBFAMILY NOT NAMEDcoord: 40..440
score: 1.4