Csa1G257930 (gene) Cucumber (Chinese Long) v2 | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Csa1G257930
Type	gene
Organism	Cucumis. sativus (Cucumber (Chinese Long) v2)
Description	mRNA, clone: RTFL01-06-A24
Location	Chr1 : 12667430 .. 12668329 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATAATTCCTCAATCTGTAATTCCCTTAAAAATTTAATGGTAGAATTCGTACCCCCCCACTTGTTCTCTGTTTCTAATGGAAAAAGGATGCGGCCGTAATTCATTTCCACAGGCATTCATTTCAGATTTTCCATAACTGTCTTAACTTCAATTGGCAATGGCGATGGTAGATTCAAGTTTTATCTTCATCGGTTTCTCCCCCGTACCTCATCCCCTTTTGAACAACAATAATGGCAGAAAATTCGGAATCACGTCGGCAAAACGTACACAAGACTCCGATTCCGATTCCGCTTCTTCAACCCAACCAAATCAATTCAAATTTCCATCTCTGAGAGTATCAAATCCACTTCTAGCCCGATCTGTCGTTTCGGTCCTCGGATTAGGGTTCGTCGATGCAGGGTACGAAAATTTCACCTCTAATTAATTTCGATTTTGAGAATTGCTCTGTTTTATTTTTGTTTTGACTGAAACTGAAGTCGAATTAGGGTTTGGAATGATTAGGTATAGCGGAGATTGGTCTAGAATTGGAGCGATTACGAAGGAAACTGAAGATTTGCTTAAGATTGGAGCTTTGTTGGTTGTTCCGTTTTGTGTATTTCTCGTATTTTCTTTCTCAAAATATGATTCGGATTCTTCGTAAGTGTATGGATGATGGATTCCCCAAAATGGTTAGAAATAATTTCAATTTGAAAATGGTAGCTAAGCCAAAGTTGCTTTTTTTGGTTAGAAGGGAGATTTTGAAGAACATGTACAAAATTCATTTACTGATATGCACATGGTTCTTGTTGGTTTCCACTCTCATGTTTTAGAATTTAGTAAGCGTCACACTGTTTAGAAATAGTCATATTCGAACAAGAAATGATATCTAGGTGCATACATTCACTTTAGTAGCTTATTGGCT

mRNA sequence

ATGGCGATGGTAGATTCAAGTTTTATCTTCATCGGTTTCTCCCCCGTACCTCATCCCCTTTTGAACAACAATAATGGCAGAAAATTCGGAATCACGTCGGCAAAACGTACACAAGACTCCGATTCCGATTCCGCTTCTTCAACCCAACCAAATCAATTCAAATTTCCATCTCTGAGAGTATCAAATCCACTTCTAGCCCGATCTGTCGTTTCGGTCCTCGGATTAGGGTTCGTCGATGCAGGGTATAGCGGAGATTGGTCTAGAATTGGAGCGATTACGAAGGAAACTGAAGATTTGCTTAAGATTGGAGCTTTGTTGGTTGTTCCGTTTTGTGTATTTCTCGTATTTTCTTTCTCAAAATATGATTCGGATTCTTCGTAA

Coding sequence (CDS)

ATGGCGATGGTAGATTCAAGTTTTATCTTCATCGGTTTCTCCCCCGTACCTCATCCCCTTTTGAACAACAATAATGGCAGAAAATTCGGAATCACGTCGGCAAAACGTACACAAGACTCCGATTCCGATTCCGCTTCTTCAACCCAACCAAATCAATTCAAATTTCCATCTCTGAGAGTATCAAATCCACTTCTAGCCCGATCTGTCGTTTCGGTCCTCGGATTAGGGTTCGTCGATGCAGGGTATAGCGGAGATTGGTCTAGAATTGGAGCGATTACGAAGGAAACTGAAGATTTGCTTAAGATTGGAGCTTTGTTGGTTGTTCCGTTTTGTGTATTTCTCGTATTTTCTTTCTCAAAATATGATTCGGATTCTTCGTAA

Protein sequence

MAMVDSSFIFIGFSPVPHPLLNNNNGRKFGITSAKRTQDSDSDSASSTQPNQFKFPSLRVSNPLLARSVVSVLGLGFVDAGYSGDWSRIGAITKETEDLLKIGALLVVPFCVFLVFSFSKYDSDSS*

Homology

[Close]

BLAST of Csa1G257930 vs. TrEMBL
Match: A0A0A0LWH4_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_1G257930 PE=4 SV=1)

HSP 1 Score: 251.1 bits (640), Expect = 7.0e-64
Identity = 126/126 (100.00%), Postives = 126/126 (100.00%), Query Frame = 1


Query: 1   MAMVDSSFIFIGFSPVPHPLLNNNNGRKFGITSAKRTQDSDSDSASSTQPNQFKFPSLRV 60
           MAMVDSSFIFIGFSPVPHPLLNNNNGRKFGITSAKRTQDSDSDSASSTQPNQFKFPSLRV
Sbjct: 1   MAMVDSSFIFIGFSPVPHPLLNNNNGRKFGITSAKRTQDSDSDSASSTQPNQFKFPSLRV 60

Query: 61  SNPLLARSVVSVLGLGFVDAGYSGDWSRIGAITKETEDLLKIGALLVVPFCVFLVFSFSK 120
           SNPLLARSVVSVLGLGFVDAGYSGDWSRIGAITKETEDLLKIGALLVVPFCVFLVFSFSK
Sbjct: 61  SNPLLARSVVSVLGLGFVDAGYSGDWSRIGAITKETEDLLKIGALLVVPFCVFLVFSFSK 120

Query: 121 YDSDSS 127
           YDSDSS
Sbjct: 121 YDSDSS 126

[Close]

BLAST of Csa1G257930 vs. TrEMBL
Match: D7U1G5_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_11s0037g00830 PE=4 SV=1)

HSP 1 Score: 120.6 bits (301), Expect = 1.4e-24
Identity = 68/120 (56.67%), Postives = 82/120 (68.33%), Query Frame = 1


Query: 1   MAMVDSSFIFIGFSPVPHPLLNNNNGRKFGITSAKRTQDSDSDSASSTQPNQFKFPSLRV 60
           M +   +FIF   + +P  L +  NG KF  T A   Q  D+   SSTQ  Q   P LRV
Sbjct: 1   MLVTQDTFIF---NTLPPYLFHIKNGTKFRPTLA---QKRDASKNSSTQQQQSILP-LRV 60

Query: 61  SNPLLARSVVSVLGLGFVDAGYSGDWSRIGAITKETEDLLKIGALLVVPFCVFLVFSFSK 120
           SN +LARS ++VLGLGF+DAGYSGDWSRIG I+KETED LK+ ALLVVP C+FL+FS SK
Sbjct: 61  SNTILARSAIAVLGLGFIDAGYSGDWSRIGVISKETEDFLKLSALLVVPLCLFLIFSISK 113

[Close]

BLAST of Csa1G257930 vs. TrEMBL
Match: A0A059CSI4_EUCGR (Uncharacterized protein OS=Eucalyptus grandis GN=EUGRSUZ_C02516 PE=4 SV=1)

HSP 1 Score: 118.2 bits (295), Expect = 7.1e-24
Identity = 69/126 (54.76%), Postives = 87/126 (69.05%), Query Frame = 1


Query: 1   MAMVDSSFIFIGFSPVP-HPLLNNNNGRKFGITSAKRTQDSDSDSASSTQPNQFKFPSLR 60
           M +  +SFIF   + VP HPL+N   GRK G   AK+   +D  S +  QP      +LR
Sbjct: 1   MVITRTSFIF---TKVPAHPLIN---GRKPGAILAKKGDLTDRSSKTRQQPGF----ALR 60

Query: 61  VSNPLLARSVVSVLGLGFVDAGYSGDWSRIGAITKETEDLLKIGALLVVPFCVFLVFSFS 120
           VSNPLL ++ ++V GLGF+DAGYSGDWSRIGAI+KE EDLLKI A +VVP C+F +FS S
Sbjct: 61  VSNPLLTKAAIAVFGLGFIDAGYSGDWSRIGAISKEGEDLLKIAAFVVVPLCIFAIFSVS 115

Query: 121 KYDSDS 126
           K DS+S
Sbjct: 121 K-DSES 115

[Close]

BLAST of Csa1G257930 vs. TrEMBL
Match: A0A058ZTX1_EUCGR (Uncharacterized protein OS=Eucalyptus grandis GN=EUGRSUZ_L01549 PE=4 SV=1)

HSP 1 Score: 117.1 bits (292), Expect = 1.6e-23
Identity = 69/126 (54.76%), Postives = 86/126 (68.25%), Query Frame = 1


Query: 1   MAMVDSSFIFIGFSPVP-HPLLNNNNGRKFGITSAKRTQDSDSDSASSTQPNQFKFPSLR 60
           M +  +SFIF   + VP HPL+N   GRK G   AK+   +D  S +  QP      +LR
Sbjct: 1   MVITRTSFIF---TKVPAHPLIN---GRKPGAILAKKGDLTDRSSKTRRQPGF----ALR 60

Query: 61  VSNPLLARSVVSVLGLGFVDAGYSGDWSRIGAITKETEDLLKIGALLVVPFCVFLVFSFS 120
           VSNPLL R+ ++V GLGF+DAGYSGDWSRIGAI+KE EDLLKI A +VVP C+F + S S
Sbjct: 61  VSNPLLTRAAIAVFGLGFIDAGYSGDWSRIGAISKEGEDLLKIAAFVVVPLCIFAILSVS 115

Query: 121 KYDSDS 126
           K DS+S
Sbjct: 121 K-DSES 115

[Close]

BLAST of Csa1G257930 vs. TrEMBL
Match: A0A0D2UZ55_GOSRA (Uncharacterized protein OS=Gossypium raimondii GN=B456_009G368800 PE=4 SV=1)

HSP 1 Score: 114.0 bits (284), Expect = 1.3e-22
Identity = 64/121 (52.89%), Postives = 81/121 (66.94%), Query Frame = 1


Query: 7   SFIFIGFSPVPHP--LLNNNNGRKFGITSAKRTQDSDSDSASSTQPNQFKFPSLRVSNPL 66
           SF    F+ +P P  L  N NG +F   ++KR ++    S +   P   KF  L+VSN +
Sbjct: 2   SFTHTIFNGIPQPSRLPVNRNGTRFITKASKRKENGGLTSRNQQLP---KF-QLKVSNTV 61

Query: 67  LARSVVSVLGLGFVDAGYSGDWSRIGAITKETEDLLKIGALLVVPFCVFLVFSFSKYDSD 126
           +ARSVV + GLGF+DAGYSGDWSRIG I+K+ EDLLKI A LVVPFC+FLV SFSK   D
Sbjct: 62  IARSVVVLFGLGFIDAGYSGDWSRIGVISKDVEDLLKIAAFLVVPFCIFLVSSFSKQTPD 118

[Close]

BLAST of Csa1G257930 vs. TAIR10
Match: AT4G30845.1 (AT4G30845.1 unknown protein)

HSP 1 Score: 99.8 bits (247), Expect = 1.3e-21
Identity = 55/117 (47.01%), Postives = 73/117 (62.39%), Query Frame = 1


Query: 10  FIGFSPVPHPLLNNNNGRKF--GITSAKRTQDSDSDSASSTQPNQFKFPSLRVSNPLLAR 69
           F+ F P      N+N  R F  G+T   + +D  S+ ++  +P       ++V N ++AR
Sbjct: 9   FVSFCP------NDNKLRSFANGVTIMSKRRDF-SEKSNEERP----ILRIKVPNTIVAR 68

Query: 70  SVVSVLGLGFVDAGYSGDWSRIGAITKETEDLLKIGALLVVPFCVFLVFSFSKYDSD 125
           S ++VL LGF+DAGYSGDWSRIG I+KETE+LLKI A LVVP C+FL  SFS   +D
Sbjct: 69  SAIAVLSLGFIDAGYSGDWSRIGVISKETEELLKIAAFLVVPLCIFLALSFSNDSTD 114

[Close]

BLAST of Csa1G257930 vs. NCBI nr
Match: gi|449457863|ref|XP_004146667.1| (PREDICTED: uncharacterized protein LOC101206545 [Cucumis sativus])

HSP 1 Score: 251.1 bits (640), Expect = 1.0e-63
Identity = 126/126 (100.00%), Postives = 126/126 (100.00%), Query Frame = 1


Query: 1   MAMVDSSFIFIGFSPVPHPLLNNNNGRKFGITSAKRTQDSDSDSASSTQPNQFKFPSLRV 60
           MAMVDSSFIFIGFSPVPHPLLNNNNGRKFGITSAKRTQDSDSDSASSTQPNQFKFPSLRV
Sbjct: 1   MAMVDSSFIFIGFSPVPHPLLNNNNGRKFGITSAKRTQDSDSDSASSTQPNQFKFPSLRV 60

Query: 61  SNPLLARSVVSVLGLGFVDAGYSGDWSRIGAITKETEDLLKIGALLVVPFCVFLVFSFSK 120
           SNPLLARSVVSVLGLGFVDAGYSGDWSRIGAITKETEDLLKIGALLVVPFCVFLVFSFSK
Sbjct: 61  SNPLLARSVVSVLGLGFVDAGYSGDWSRIGAITKETEDLLKIGALLVVPFCVFLVFSFSK 120

Query: 121 YDSDSS 127
           YDSDSS
Sbjct: 121 YDSDSS 126

[Close]

BLAST of Csa1G257930 vs. NCBI nr
Match: gi|659086235|ref|XP_008443827.1| (PREDICTED: uncharacterized protein LOC103487329 [Cucumis melo])

HSP 1 Score: 225.3 bits (573), Expect = 5.9e-56
Identity = 118/129 (91.47%), Postives = 120/129 (93.02%), Query Frame = 1


Query: 1   MAMVDSSFIFIGFSPVPHPLLNNN---NGRKFGITSAKRTQDSDSDSASSTQPNQFKFPS 60
           MAMVDSSFIFIG S VPHPLLNNN   NGRKFGITSAKRTQDSDS   SSTQPNQFKFPS
Sbjct: 1   MAMVDSSFIFIGISAVPHPLLNNNKNNNGRKFGITSAKRTQDSDS--TSSTQPNQFKFPS 60

Query: 61  LRVSNPLLARSVVSVLGLGFVDAGYSGDWSRIGAITKETEDLLKIGALLVVPFCVFLVFS 120
           LRVSNPLLARSVVSVLGLGFVDAGYSGDWSRIGAITKETE+LLKIGA LVVPFCVFLVFS
Sbjct: 61  LRVSNPLLARSVVSVLGLGFVDAGYSGDWSRIGAITKETEELLKIGAFLVVPFCVFLVFS 120

Query: 121 FSKYDSDSS 127
           FSK+DSDSS
Sbjct: 121 FSKFDSDSS 127

[Close]

BLAST of Csa1G257930 vs. NCBI nr
Match: gi|694419824|ref|XP_009337849.1| (PREDICTED: uncharacterized protein LOC103930267 [Pyrus x bretschneideri])

HSP 1 Score: 121.7 bits (304), Expect = 9.2e-25
Identity = 65/126 (51.59%), Postives = 85/126 (67.46%), Query Frame = 1


Query: 1   MAMVDSSFIFIGFSPV-PHPLLNNNNGRKFGITSAKRTQDSDSDSASSTQPNQFKFP-SL 60
           M + + S IF     + P+ LL   NGR+FG T     ++   + +  +Q  Q  FP  +
Sbjct: 1   MLVAEKSLIFSSSRNLSPYLLLKRKNGREFGSTFLLAKREDMPEDSRRSQDQQSVFPLRI 60

Query: 61  RVSNPLLARSVVSVLGLGFVDAGYSGDWSRIGAITKETEDLLKIGALLVVPFCVFLVFSF 120
           RVSN +LAR+ V+V GLGF+DAGYSGDWSRIGAI+KE+E+LLKI A LVVPFC+FL+ SF
Sbjct: 61  RVSNTVLARAAVAVFGLGFIDAGYSGDWSRIGAISKESEELLKISAFLVVPFCLFLIISF 120

Query: 121 SKYDSD 125
           SK   D
Sbjct: 121 SKEAED 126

[Close]

BLAST of Csa1G257930 vs. NCBI nr
Match: gi|470148842|ref|XP_004309956.1| (PREDICTED: uncharacterized protein LOC101291784 [Fragaria vesca subsp. vesca])

HSP 1 Score: 121.7 bits (304), Expect = 9.2e-25
Identity = 64/105 (60.95%), Postives = 76/105 (72.38%), Query Frame = 1


Query: 20  LLNNNNGRKFGITSAKRTQDSDSDSASSTQPNQFKFPSLRVSNPLLARSVVSVLGLGFVD 79
           L    NGR  G   AKR +D  ++  SSTQ   F FP LRVSN ++ARSVV+VLGLGF+D
Sbjct: 17  LFVKKNGRDLGTIMAKR-RDLPTE-ISSTQEGGFVFPRLRVSNTIVARSVVAVLGLGFID 76

Query: 80  AGYSGDWSRIGAITKETEDLLKIGALLVVPFCVFLVFSFSKYDSD 125
           AGYSGDWSR G I+KE+EDLLK+ A LVVP C+FL+FS SK   D
Sbjct: 77  AGYSGDWSRFGVISKESEDLLKVAAFLVVPLCLFLIFSISKQHED 119

[Close]

BLAST of Csa1G257930 vs. NCBI nr
Match: gi|225445770|ref|XP_002274551.1| (PREDICTED: uncharacterized protein LOC100267275 [Vitis vinifera])

HSP 1 Score: 120.6 bits (301), Expect = 2.1e-24
Identity = 68/120 (56.67%), Postives = 82/120 (68.33%), Query Frame = 1


Query: 1   MAMVDSSFIFIGFSPVPHPLLNNNNGRKFGITSAKRTQDSDSDSASSTQPNQFKFPSLRV 60
           M +   +FIF   + +P  L +  NG KF  T A   Q  D+   SSTQ  Q   P LRV
Sbjct: 1   MLVTQDTFIF---NTLPPYLFHIKNGTKFRPTLA---QKRDASKNSSTQQQQSILP-LRV 60

Query: 61  SNPLLARSVVSVLGLGFVDAGYSGDWSRIGAITKETEDLLKIGALLVVPFCVFLVFSFSK 120
           SN +LARS ++VLGLGF+DAGYSGDWSRIG I+KETED LK+ ALLVVP C+FL+FS SK
Sbjct: 61  SNTILARSAIAVLGLGFIDAGYSGDWSRIGVISKETEDFLKLSALLVVPLCLFLIFSISK 113

The following BLAST results are available for this feature:

BLAST of Csa1G257930 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LWH4_CUCSA	7.0e-64	100.00	Uncharacterized protein OS=Cucumis sativus GN=Csa_1G257930 PE=4 SV=1	[more]
D7U1G5_VITVI	1.4e-24	56.67	Putative uncharacterized protein OS=Vitis vinifera GN=VIT_11s0037g00830 PE=4 SV=...	[more]
A0A059CSI4_EUCGR	7.1e-24	54.76	Uncharacterized protein OS=Eucalyptus grandis GN=EUGRSUZ_C02516 PE=4 SV=1	[more]
A0A058ZTX1_EUCGR	1.6e-23	54.76	Uncharacterized protein OS=Eucalyptus grandis GN=EUGRSUZ_L01549 PE=4 SV=1	[more]
A0A0D2UZ55_GOSRA	1.3e-22	52.89	Uncharacterized protein OS=Gossypium raimondii GN=B456_009G368800 PE=4 SV=1	[more]

BLAST of Csa1G257930 vs. TAIR10


Match Name	E-value	Identity	Description
AT4G30845.1	1.3e-21	47.01	unknown protein	[more]

BLAST of Csa1G257930 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|449457863\|ref\|XP_004146667.1\|	1.0e-63	100.00	PREDICTED: uncharacterized protein LOC101206545 [Cucumis sativus]	[more]
gi\|659086235\|ref\|XP_008443827.1\|	5.9e-56	91.47	PREDICTED: uncharacterized protein LOC103487329 [Cucumis melo]	[more]
gi\|694419824\|ref\|XP_009337849.1\|	9.2e-25	51.59	PREDICTED: uncharacterized protein LOC103930267 [Pyrus x bretschneideri]	[more]
gi\|470148842\|ref\|XP_004309956.1\|	9.2e-25	60.95	PREDICTED: uncharacterized protein LOC101291784 [Fragaria vesca subsp. vesca]	[more]
gi\|225445770\|ref\|XP_002274551.1\|	2.1e-24	56.67	PREDICTED: uncharacterized protein LOC100267275 [Vitis vinifera]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006144	purine nucleobase metabolic process
biological_process	GO:0006206	pyrimidine nucleobase metabolic process
biological_process	GO:0006351	transcription, DNA-templated
biological_process	GO:0008150	biological_process
cellular_component	GO:0005730	nucleolus
cellular_component	GO:0005575	cellular_component
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0003899	DNA-directed RNA polymerase activity
molecular_function	GO:0005515	protein binding
molecular_function	GO:0003674	molecular_function

Unigene

This gene is associated with the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
CU137223	cucumber EST collection version 3.0	transcribed_cluster
CU158510	cucumber EST collection version 3.0	transcribed_cluster
CU173462	cucumber EST collection version 3.0	transcribed_cluster

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Csa1G257930.1	Csa1G257930.1	mRNA

The following transcribed_cluster feature(s) are associated with this gene:


Feature Name	Unique Name	Type
CU173462	CU173462	transcribed_cluster
CU137223	CU137223	transcribed_cluster
CU158510	CU158510	transcribed_cluster

InterPro

Analysis Name: InterPro Annotations of cucumber (Chinese Long)
Date Performed: 2016-09-28


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	PANTHER	PTHR38389	FAMILY NOT NAMED	coord: 10..124 score: 7.3

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Csa1G257930	Cla018756	Watermelon (97103) v1	cuwmB085
Csa1G257930	MELO3C010198	Melon (DHL92) v3.5.1	cumeB060
Csa1G257930	ClCG06G012660	Watermelon (Charleston Gray)	cuwcgB072
Csa1G257930	Lsi06G010810	Bottle gourd (USVL1VR-Ls)	culsiB073
Csa1G257930	MELO3C010198.2	Melon (DHL92) v3.6.1	cumedB053
Csa1G257930	Cla97C06G121880	Watermelon (97103) v2	cuwmbB071
Csa1G257930	Bhi02G000657	Wax gourd	cuwgoB103
Csa1G257930	CSPI01G17990	Wild cucumber (PI 183967)	cpicuB004
Csa1G257930	Cucsa.170760	Cucumber (Gy14) v1	cgycuB260
Csa1G257930	CmaCh11G004400	Cucurbita maxima (Rimu)	cmacuB102
Csa1G257930	CmoCh11G004400	Cucurbita moschata (Rifu)	cmocuB091
Csa1G257930	Cp4.1LG04g12610	Cucurbita pepo (Zucchini)	cpecuB641
Csa1G257930	CsGy1G018060	Cucumber (Gy14) v2	cgybcuB006
Csa1G257930	Carg14071	Silver-seed gourd	carcuB0532

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Csa1G257930	Cucumber (Chinese Long) v2	cucuB009
Csa1G257930	Cucumber (Gy14) v1	cgycuB095
Csa1G257930	Bottle gourd (USVL1VR-Ls)	culsiB011
Csa1G257930	Bottle gourd (USVL1VR-Ls)	culsiB066
Csa1G257930	Cucumber (Gy14) v2	cgybcuB008