CsGy6G025650 (gene) Cucumber (Gy14) v2

NameCsGy6G025650
Typegene
OrganismCucumis sativus (Cucumber (Gy14) v2)
DescriptionCysteine proteinase inhibitor
LocationChr6 : 25179072 .. 25179705 (-)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTGTACTCCTCTGGGTCCTTATGGTCCATGTGACGATTCGGATGCCCCATACGTAAAAGAAATTGCAGAACGGGCAGTGGCTGATCACAACAAATCAGCAGGAACAAATTACACACTCGTTTCCATCGTCAACTGTGAGTCCGTGGTTGTATCCGGAACCAACTACCGCCTTGTGCTGTCGCTTAAGGATGACAGTACTACCAGTGATTTCTTGGTAGTTGTGTATTATAGGCCATGGGATAATTACCTGGAGGTCACTCAGTTTGAACCTGTCACGAAATAAATTGCAACATCCCATCTACTACTGCTTATATATATACGTATGTATGAGCTATCTATATAAGTAATGGAACATACATTTTAAATAAAGCTATTATGTGTGTGTTTTTGTATCTTTTCATCTCACTGTTGTGGGGCGCATGTTTAGAAGTTCTTTTTTTTCTTTCAAAGGGGTGCAAACAGGAGAATTTCTCATCTAAGGAAGCTTAGACTATGCGAGTTTTGATGGAATTTAGTGTGAATTAGTGCAGGTATAATCGTGCCACAAAATATATAGTTTGGTCTTTTTATATGAGAAATGAAATATGTAAATCTCACTGTACTTGTGGCTGCAGGTTGAATGTATCAATTGA

mRNA sequence

ATGTGTACTCCTCTGGGTCCTTATGGTCCATGTGACGATTCGGATGCCCCATACGTAAAAGAAATTGCAGAACGGGCAGTGGCTGATCACAACAAATCAGCAGGAACAAATTACACACTCGTTTCCATCGTCAACTGTGAGTCCGTGGTTGTATCCGGAACCAACTACCGCCTTGTGCTGTCGCTTAAGGATGACAGTACTACCAGTGATTTCTTGGTAGTTGTGTATTATAGGCCATGGGATAATTACCTGGAGGTTGAATGTATCAATTGA

Coding sequence (CDS)

ATGTGTACTCCTCTGGGTCCTTATGGTCCATGTGACGATTCGGATGCCCCATACGTAAAAGAAATTGCAGAACGGGCAGTGGCTGATCACAACAAATCAGCAGGAACAAATTACACACTCGTTTCCATCGTCAACTGTGAGTCCGTGGTTGTATCCGGAACCAACTACCGCCTTGTGCTGTCGCTTAAGGATGACAGTACTACCAGTGATTTCTTGGTAGTTGTGTATTATAGGCCATGGGATAATTACCTGGAGGTTGAATGTATCAATTGA

Protein sequence

MCTPLGPYGPCDDSDAPYVKEIAERAVADHNKSAGTNYTLVSIVNCESVVVSGTNYRLVLSLKDDSTTSDFLVVVYYRPWDNYLEVECIN
BLAST of CsGy6G025650 vs. NCBI nr
Match: KGN48311.1 (hypothetical protein Csa_6G476020 [Cucumis sativus])

HSP 1 Score: 180.6 bits (457), Expect = 2.3e-42
Identity = 86/86 (100.00%), Postives = 86/86 (100.00%), Query Frame = 0

Query: 1  MCTPLGPYGPCDDSDAPYVKEIAERAVADHNKSAGTNYTLVSIVNCESVVVSGTNYRLVL 60
          MCTPLGPYGPCDDSDAPYVKEIAERAVADHNKSAGTNYTLVSIVNCESVVVSGTNYRLVL
Sbjct: 1  MCTPLGPYGPCDDSDAPYVKEIAERAVADHNKSAGTNYTLVSIVNCESVVVSGTNYRLVL 60

Query: 61 SLKDDSTTSDFLVVVYYRPWDNYLEV 87
          SLKDDSTTSDFLVVVYYRPWDNYLEV
Sbjct: 61 SLKDDSTTSDFLVVVYYRPWDNYLEV 86

BLAST of CsGy6G025650 vs. NCBI nr
Match: NP_001267677.1 (cysteine proteinase inhibitor 5-like [Cucumis sativus] >BAA28867.1 cystein proteinase inhibitor [Cucumis sativus])

HSP 1 Score: 81.6 bits (200), Expect = 1.5e-12
Identity = 40/83 (48.19%), Postives = 60/83 (72.29%), Query Frame = 0

Query: 5  LGPYGPCDDSDAPYVKEIAERAVADHNKSAGTNYTLVSIVNCESVVVSGTNYRLVLSLKD 64
          +G Y PC D + P+VK+IAE AVA++NKS G + TLVSI+ CES VV+G N+RLVL  KD
Sbjct: 6  IGGYVPCKDPNDPHVKDIAEWAVAEYNKSQGHHLTLVSILKCESQVVAGVNWRLVLKCKD 65

Query: 65 DST-TSDFLVVVYYRPWDNYLEV 87
          ++    ++  VV+ + W+N+ ++
Sbjct: 66 ENNGEGNYETVVWEKIWENFRQL 88

BLAST of CsGy6G025650 vs. NCBI nr
Match: AFK40971.1 (unknown [Lotus japonicus])

HSP 1 Score: 77.0 bits (188), Expect = 3.6e-11
Identity = 35/82 (42.68%), Postives = 55/82 (67.07%), Query Frame = 0

Query: 5   LGPYGPCDDSDAPYVKEIAERAVADHNKSAGTNYTLVSIVNCESVVVSGTNYRLVLSLKD 64
           +G Y P  D +AP+V++IA+ A+ +HNK  G N  L  ++  +S VVSG NYRL L+  D
Sbjct: 22  VGGYSPIKDINAPHVRKIAQFAITEHNKHTGENLKLERVIKGDSQVVSGLNYRLTLAATD 81

Query: 65  DSTTSDFLVVVYYRPWDNYLEV 87
            ST+ ++LV+VY + W + +E+
Sbjct: 82  GSTSHNYLVIVYEQSWTHTIEL 103

BLAST of CsGy6G025650 vs. NCBI nr
Match: XP_009396391.1 (PREDICTED: cysteine proteinase inhibitor 1-like [Musa acuminata subsp. malaccensis])

HSP 1 Score: 75.1 bits (183), Expect = 1.4e-10
Identity = 38/83 (45.78%), Postives = 56/83 (67.47%), Query Frame = 0

Query: 5   LGPYGPCDDSDAPYVKEIAERAVADHNKSAGTNYTLVSIVNCESVVVSGTNYRLVLSLKD 64
           LG + P  ++D P+V+EIAE AVA+HNK A  +  L  +V  E+ VV+GTNYRLVL +KD
Sbjct: 32  LGGWTPIKNTDDPHVREIAEFAVAEHNKQANASLALSKVVKGETQVVAGTNYRLVLQVKD 91

Query: 65  DS-TTSDFLVVVYYRPWDNYLEV 87
            S     +  VV+ +PW+N+ ++
Sbjct: 92  ASGARVKYQAVVWEKPWENFRQL 114

BLAST of CsGy6G025650 vs. NCBI nr
Match: XP_011008977.1 (PREDICTED: cysteine proteinase inhibitor 5-like [Populus euphratica])

HSP 1 Score: 74.3 bits (181), Expect = 2.3e-10
Identity = 41/78 (52.56%), Postives = 54/78 (69.23%), Query Frame = 0

Query: 5   LGPYGPCDDSDAPYVKEIAERAVADHNKSAGTNYTLVSIVNCESVVVSGTNYRLVLSLKD 64
           LG + P  D    +V EIAE AVA++NK A +N TLVSIV  ES VVSGTNYRLVL++K 
Sbjct: 25  LGGWSPIKDLKDKHVVEIAEFAVAEYNKEAKSNLTLVSIVKGESQVVSGTNYRLVLAVK- 84

Query: 65  DSTTSDFLVVVYYRPWDN 83
              ++ +  VVY +PW++
Sbjct: 85  GGASAKYQAVVYEKPWEH 101

BLAST of CsGy6G025650 vs. TAIR10
Match: AT5G47550.1 (Cystatin/monellin superfamily protein)

HSP 1 Score: 56.2 bits (134), Expect = 1.2e-08
Identity = 28/77 (36.36%), Postives = 48/77 (62.34%), Query Frame = 0

Query: 5   LGPYGPCDDSDAPYVKEIAERAVADHNKSAGTNYTLVSIVNCESVVVSGTNYRLVLSLKD 64
           +G + P  +   P V EI E AV+++NK + +     ++V+ E+ VVSGTNYRL ++  D
Sbjct: 28  VGGWSPISNVTDPQVVEIGEFAVSEYNKRSESGLKFETVVSGETQVVSGTNYRLKVAAND 87

Query: 65  -DSTTSDFLVVVYYRPW 81
            D  + ++L +V+ +PW
Sbjct: 88  GDGVSKNYLAIVWDKPW 104

BLAST of CsGy6G025650 vs. Swiss-Prot
Match: sp|Q6TPK4|CYT1_ACTDE (Cysteine proteinase inhibitor 1 OS=Actinidia deliciosa OX=3627 PE=1 SV=1)

HSP 1 Score: 58.5 bits (140), Expect = 4.3e-08
Identity = 27/78 (34.62%), Postives = 49/78 (62.82%), Query Frame = 0

Query: 6   GPYGPCDDSDAPYVKEIAERAVADHNKSAGTNYTLVSIVNCESVVVSGTNYRLVLSLKDD 65
           G + P +  ++  V+++A+ AV++HNK A       S+V   + VV+GTNYRLV++ KD 
Sbjct: 30  GGWRPIESLNSAEVQDVAQFAVSEHNKQANDELQYQSVVRGYTQVVAGTNYRLVIAAKDG 89

Query: 66  STTSDFLVVVYYRPWDNY 84
           +   ++  VV+ +PW ++
Sbjct: 90  AVVGNYEAVVWDKPWMHF 107

BLAST of CsGy6G025650 vs. Swiss-Prot
Match: sp|Q41916|CYT5_ARATH (Cysteine proteinase inhibitor 5 OS=Arabidopsis thaliana OX=3702 GN=CYS5 PE=2 SV=2)

HSP 1 Score: 56.2 bits (134), Expect = 2.1e-07
Identity = 28/77 (36.36%), Postives = 48/77 (62.34%), Query Frame = 0

Query: 5   LGPYGPCDDSDAPYVKEIAERAVADHNKSAGTNYTLVSIVNCESVVVSGTNYRLVLSLKD 64
           +G + P  +   P V EI E AV+++NK + +     ++V+ E+ VVSGTNYRL ++  D
Sbjct: 28  VGGWSPISNVTDPQVVEIGEFAVSEYNKRSESGLKFETVVSGETQVVSGTNYRLKVAAND 87

Query: 65  -DSTTSDFLVVVYYRPW 81
            D  + ++L +V+ +PW
Sbjct: 88  GDGVSKNYLAIVWDKPW 104

BLAST of CsGy6G025650 vs. Swiss-Prot
Match: sp|Q10J94|CYT8_ORYSJ (Cysteine proteinase inhibitor 8 OS=Oryza sativa subsp. japonica OX=39947 GN=Os03g0429000 PE=2 SV=1)

HSP 1 Score: 48.1 bits (113), Expect = 5.8e-05
Identity = 26/79 (32.91%), Postives = 41/79 (51.90%), Query Frame = 0

Query: 5   LGPYGPCDDSDAPYVKEIAERAVADHNKSAGTNYTLVSIVNCESVVVSGTNYRLVLSLKD 64
           +G + P  D   P+++E+   AV  H   +        + + E  VVSG NYRLV+S  D
Sbjct: 32  VGGWSPITDVGDPHIQELGGWAVERHASLSSDGLRFRRVTSGEQQVVSGMNYRLVVSASD 91

Query: 65  DS-TTSDFLVVVYYRPWDN 83
            +  T+ ++ VVY + W N
Sbjct: 92  PAGATASYVAVVYEQSWTN 110

BLAST of CsGy6G025650 vs. Swiss-Prot
Match: sp|P31726|CYT1_MAIZE (Cystatin-1 OS=Zea mays OX=4577 GN=RAMDAZC7 PE=2 SV=1)

HSP 1 Score: 45.8 bits (107), Expect = 2.9e-04
Identity = 22/78 (28.21%), Postives = 44/78 (56.41%), Query Frame = 0

Query: 10  PCDDSDAPYVKEIAERAVADHNKSAGTNYTLVSIVNCESVVVSGTNYRLVLSLKDDSTTS 69
           P +++D   ++E+A  AV +HN+ A        +V  ++ VV+GT Y L + +KD     
Sbjct: 48  PANENDL-QLQELARFAVNEHNQKANALLGFEKLVKAKTQVVAGTMYYLTIEVKDGEVKK 107

Query: 70  DFLVVVYYRPWDNYLEVE 88
            +   V+ +PW+N+ +++
Sbjct: 108 LYEAKVWEKPWENFKQLQ 124

BLAST of CsGy6G025650 vs. TrEMBL
Match: tr|A0A0A0KKN2|A0A0A0KKN2_CUCSA (Cysteine proteinase inhibitor OS=Cucumis sativus OX=3659 GN=Csa_6G476020 PE=3 SV=1)

HSP 1 Score: 180.6 bits (457), Expect = 1.5e-42
Identity = 86/86 (100.00%), Postives = 86/86 (100.00%), Query Frame = 0

Query: 1  MCTPLGPYGPCDDSDAPYVKEIAERAVADHNKSAGTNYTLVSIVNCESVVVSGTNYRLVL 60
          MCTPLGPYGPCDDSDAPYVKEIAERAVADHNKSAGTNYTLVSIVNCESVVVSGTNYRLVL
Sbjct: 1  MCTPLGPYGPCDDSDAPYVKEIAERAVADHNKSAGTNYTLVSIVNCESVVVSGTNYRLVL 60

Query: 61 SLKDDSTTSDFLVVVYYRPWDNYLEV 87
          SLKDDSTTSDFLVVVYYRPWDNYLEV
Sbjct: 61 SLKDDSTTSDFLVVVYYRPWDNYLEV 86

BLAST of CsGy6G025650 vs. TrEMBL
Match: tr|O80389|O80389_CUCSA (Cysteine proteinase inhibitor OS=Cucumis sativus OX=3659 PE=2 SV=1)

HSP 1 Score: 81.6 bits (200), Expect = 9.6e-13
Identity = 40/83 (48.19%), Postives = 60/83 (72.29%), Query Frame = 0

Query: 5  LGPYGPCDDSDAPYVKEIAERAVADHNKSAGTNYTLVSIVNCESVVVSGTNYRLVLSLKD 64
          +G Y PC D + P+VK+IAE AVA++NKS G + TLVSI+ CES VV+G N+RLVL  KD
Sbjct: 6  IGGYVPCKDPNDPHVKDIAEWAVAEYNKSQGHHLTLVSILKCESQVVAGVNWRLVLKCKD 65

Query: 65 DST-TSDFLVVVYYRPWDNYLEV 87
          ++    ++  VV+ + W+N+ ++
Sbjct: 66 ENNGEGNYETVVWEKIWENFRQL 88

BLAST of CsGy6G025650 vs. TrEMBL
Match: tr|I3SL29|I3SL29_LOTJA (Cysteine proteinase inhibitor OS=Lotus japonicus OX=34305 PE=2 SV=1)

HSP 1 Score: 77.0 bits (188), Expect = 2.4e-11
Identity = 35/82 (42.68%), Postives = 55/82 (67.07%), Query Frame = 0

Query: 5   LGPYGPCDDSDAPYVKEIAERAVADHNKSAGTNYTLVSIVNCESVVVSGTNYRLVLSLKD 64
           +G Y P  D +AP+V++IA+ A+ +HNK  G N  L  ++  +S VVSG NYRL L+  D
Sbjct: 22  VGGYSPIKDINAPHVRKIAQFAITEHNKHTGENLKLERVIKGDSQVVSGLNYRLTLAATD 81

Query: 65  DSTTSDFLVVVYYRPWDNYLEV 87
            ST+ ++LV+VY + W + +E+
Sbjct: 82  GSTSHNYLVIVYEQSWTHTIEL 103

BLAST of CsGy6G025650 vs. TrEMBL
Match: tr|A0A1D1YNQ2|A0A1D1YNQ2_9ARAE (Cysteine proteinase inhibitor (Fragment) OS=Anthurium amnicola OX=1678845 GN=CYS5 PE=3 SV=1)

HSP 1 Score: 75.9 bits (185), Expect = 5.3e-11
Identity = 39/86 (45.35%), Postives = 49/86 (56.98%), Query Frame = 0

Query: 5   LGPYGPCDDSDAPYVKEIAERAVADHNKSAGTNYTLVSIVNCESVVVSGTNYRLVLSLKD 64
           LG Y P  +   PYV+EI + AVA+HNK AG     V +V+ E  VV+GTNY+LVL   D
Sbjct: 55  LGGYQPIRNVGDPYVREIGQFAVAEHNKEAGQALAFVRVVSGEQQVVAGTNYKLVLQASD 114

Query: 65  DSTTSDFLVVVYYRPWDNYLEVECIN 91
              T  +  VVY RPWD    +   N
Sbjct: 115 RGATKAYEAVVYDRPWDKVRTLTSFN 140

BLAST of CsGy6G025650 vs. TrEMBL
Match: tr|M0SNR0|M0SNR0_MUSAM (Cysteine proteinase inhibitor OS=Musa acuminata subsp. malaccensis OX=214687 GN=103981394 PE=3 SV=1)

HSP 1 Score: 75.1 bits (183), Expect = 9.0e-11
Identity = 38/83 (45.78%), Postives = 56/83 (67.47%), Query Frame = 0

Query: 5   LGPYGPCDDSDAPYVKEIAERAVADHNKSAGTNYTLVSIVNCESVVVSGTNYRLVLSLKD 64
           LG + P  ++D P+V+EIAE AVA+HNK A  +  L  +V  E+ VV+GTNYRLVL +KD
Sbjct: 32  LGGWTPIKNTDDPHVREIAEFAVAEHNKQANASLALSKVVKGETQVVAGTNYRLVLQVKD 91

Query: 65  DS-TTSDFLVVVYYRPWDNYLEV 87
            S     +  VV+ +PW+N+ ++
Sbjct: 92  ASGARVKYQAVVWEKPWENFRQL 114

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KGN48311.12.3e-42100.00hypothetical protein Csa_6G476020 [Cucumis sativus][more]
NP_001267677.11.5e-1248.19cysteine proteinase inhibitor 5-like [Cucumis sativus] >BAA28867.1 cystein prote... [more]
AFK40971.13.6e-1142.68unknown [Lotus japonicus][more]
XP_009396391.11.4e-1045.78PREDICTED: cysteine proteinase inhibitor 1-like [Musa acuminata subsp. malaccens... [more]
XP_011008977.12.3e-1052.56PREDICTED: cysteine proteinase inhibitor 5-like [Populus euphratica][more]
Match NameE-valueIdentityDescription
AT5G47550.11.2e-0836.36Cystatin/monellin superfamily protein[more]
Match NameE-valueIdentityDescription
sp|Q6TPK4|CYT1_ACTDE4.3e-0834.62Cysteine proteinase inhibitor 1 OS=Actinidia deliciosa OX=3627 PE=1 SV=1[more]
sp|Q41916|CYT5_ARATH2.1e-0736.36Cysteine proteinase inhibitor 5 OS=Arabidopsis thaliana OX=3702 GN=CYS5 PE=2 SV=... [more]
sp|Q10J94|CYT8_ORYSJ5.8e-0532.91Cysteine proteinase inhibitor 8 OS=Oryza sativa subsp. japonica OX=39947 GN=Os03... [more]
sp|P31726|CYT1_MAIZE2.9e-0428.21Cystatin-1 OS=Zea mays OX=4577 GN=RAMDAZC7 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
tr|A0A0A0KKN2|A0A0A0KKN2_CUCSA1.5e-42100.00Cysteine proteinase inhibitor OS=Cucumis sativus OX=3659 GN=Csa_6G476020 PE=3 SV... [more]
tr|O80389|O80389_CUCSA9.6e-1348.19Cysteine proteinase inhibitor OS=Cucumis sativus OX=3659 PE=2 SV=1[more]
tr|I3SL29|I3SL29_LOTJA2.4e-1142.68Cysteine proteinase inhibitor OS=Lotus japonicus OX=34305 PE=2 SV=1[more]
tr|A0A1D1YNQ2|A0A1D1YNQ2_9ARAE5.3e-1145.35Cysteine proteinase inhibitor (Fragment) OS=Anthurium amnicola OX=1678845 GN=CYS... [more]
tr|M0SNR0|M0SNR0_MUSAM9.0e-1145.78Cysteine proteinase inhibitor OS=Musa acuminata subsp. malaccensis OX=214687 GN=... [more]
The following terms have been associated with this gene:
Vocabulary: Molecular Function
TermDefinition
GO:0004869cysteine-type endopeptidase inhibitor activity
Vocabulary: INTERPRO
TermDefinition
IPR027214Cystatin
IPR000010Cystatin_dom
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:2000117 negative regulation of cysteine-type endopeptidase activity
cellular_component GO:0005575 cellular_component
molecular_function GO:0004869 cysteine-type endopeptidase inhibitor activity
molecular_function GO:0002020 protease binding

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CsGy6G025650.1CsGy6G025650.1mRNA


Analysis Name: InterPro Annotations of cucumber Gy14 genome (v2)
Date Performed: 2018-09-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000010Cystatin domainPFAMPF16845SQAPIcoord: 13..86
e-value: 2.0E-17
score: 63.2
IPR000010Cystatin domainCDDcd00042CYcoord: 10..86
e-value: 5.15377E-8
score: 44.7724
NoneNo IPR availableGENE3DG3DSA:3.10.450.10coord: 3..90
e-value: 3.6E-20
score: 73.9
NoneNo IPR availablePRODOMPD001231coord: 18..86
e-value: 1.0E-12
score: 170.0
NoneNo IPR availableSUPERFAMILYSSF54403Cystatin/monellincoord: 4..87
IPR027214CystatinPANTHERPTHR11413CYSTATIN FAMILY MEMBERcoord: 6..86