Csa6G476020 (gene) Cucumber (Chinese Long) v2

NameCsa6G476020
Typegene
OrganismCucumis. sativus (Cucumber (Chinese Long) v2)
DescriptionCysteine proteinase inhibitor, putative; contains IPR000010 (Proteinase inhibitor I25, cystatin)
LocationChr6 : 21901114 .. 21901971 (+)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CATATTACTATAAATTTGAAACAATTTTTTTTATTGGAAAGAAGCACACAAATTAAAGCATTCCCTTATTAAGTAGTAATAATTTTGAAAGGGTAATTTAGAAAGATACATAGATATGTGTACTCCTCTGGGTCCTTATGGTCCATGTGACGATTCGGATGCCCCATACGTAAAAGAAATTGCAGAACGGGCAGTGGCTGATCACAACAAATCAGCAGGAACAAATTACACACTCGTTTCCATCGTCAACTGTGAGTCCGTGGTTGTATCCGGAACCAACTACCGCCTTGTGCTGTCGCTTAAGGATGACAGTACTACCAGTGATTTCTTGGTAGTTGTGTATTATAGGCCATGGGATAATTACCTGGAGGTCACTCAGTTTGAACCTGTCACGAAATAAATTGCAACATCCCATCTACTACTGCTTATATATATACGTATGTATGAGCTATCTATATAAGTAATGGAACATACATTTTAAATAAAGCTATTATGTGTGTGTTTTTGTATCTTTTCATCTCACTGTTGTGGGGCGCATGTTTAGAAGTTCTTTTTTTTCTTTCAAAGGGGTGCAAACAGGAGAATTTCTCATCTAAGGAAGCTTAGACTATGCGAGTTTTGATGGAATTTAGTGTGAATTAGTGCAGGTATAATCGTGCCACAAAATATATAGTTTGGTCTTTTTATATGAGAAATGAAATATGTAAATCTCACTGTACTTGTGGCTGCAGGTTGAATGTATCAATTGATCAATAACATAATCATATGAAAATTAAGGGTTAAGGATACAAATATGTATCTAAAGGGAAAAAATGAAATAAAATCATATACTTTTTAAGTATGTATTGAGAGCAAAAC

mRNA sequence

ATGTGTACTCCTCTGGGTCCTTATGGTCCATGTGACGATTCGGATGCCCCATACGTAAAAGAAATTGCAGAACGGGCAGTGGCTGATCACAACAAATCAGCAGGAACAAATTACACACTCGTTTCCATCGTCAACTGTGAGTCCGTGGTTGTATCCGGAACCAACTACCGCCTTGTGCTGTCGCTTAAGGATGACAGTACTACCAGTGATTTCTTGGTAGTTGTGTATTATAGGCCATGGGATAATTACCTGGAGGTCACTCAGTTTGAACCTGTCACGAAATAA

Coding sequence (CDS)

ATGTGTACTCCTCTGGGTCCTTATGGTCCATGTGACGATTCGGATGCCCCATACGTAAAAGAAATTGCAGAACGGGCAGTGGCTGATCACAACAAATCAGCAGGAACAAATTACACACTCGTTTCCATCGTCAACTGTGAGTCCGTGGTTGTATCCGGAACCAACTACCGCCTTGTGCTGTCGCTTAAGGATGACAGTACTACCAGTGATTTCTTGGTAGTTGTGTATTATAGGCCATGGGATAATTACCTGGAGGTCACTCAGTTTGAACCTGTCACGAAATAA

Protein sequence

MCTPLGPYGPCDDSDAPYVKEIAERAVADHNKSAGTNYTLVSIVNCESVVVSGTNYRLVLSLKDDSTTSDFLVVVYYRPWDNYLEVTQFEPVTK*
BLAST of Csa6G476020 vs. Swiss-Prot
Match: CYT5_ARATH (Cysteine proteinase inhibitor 5 OS=Arabidopsis thaliana GN=CYS5 PE=2 SV=2)

HSP 1 Score: 65.5 bits (158), Expect = 3.7e-10
Identity = 32/88 (36.36%), Postives = 52/88 (59.09%), Query Frame = 1

Query: 5   LGPYGPCDDSDAPYVKEIAERAVADHNKSAGTNYTLVSIVNCESVVVSGTNYRLVLSLKD 64
           +G + P  +   P V EI E AV+++NK + +     ++V+ E+ VVSGTNYRL ++  D
Sbjct: 28  VGGWSPISNVTDPQVVEIGEFAVSEYNKRSESGLKFETVVSGETQVVSGTNYRLKVAAND 87

Query: 65  -DSTTSDFLVVVYYRPWDNYLEVTQFEP 92
            D  + ++L +V+ +PW  +  +T FEP
Sbjct: 88  GDGVSKNYLAIVWDKPWMKFRNLTSFEP 115

BLAST of Csa6G476020 vs. Swiss-Prot
Match: CYT1_ACTDE (Cysteine proteinase inhibitor 1 OS=Actinidia deliciosa PE=1 SV=1)

HSP 1 Score: 64.7 bits (156), Expect = 6.3e-10
Identity = 30/87 (34.48%), Postives = 51/87 (58.62%), Query Frame = 1

Query: 6   GPYGPCDDSDAPYVKEIAERAVADHNKSAGTNYTLVSIVNCESVVVSGTNYRLVLSLKDD 65
           G + P +  ++  V+++A+ AV++HNK A       S+V   + VV+GTNYRLV++ KD 
Sbjct: 30  GGWRPIESLNSAEVQDVAQFAVSEHNKQANDELQYQSVVRGYTQVVAGTNYRLVIAAKDG 89

Query: 66  STTSDFLVVVYYRPWDNYLEVTQFEPV 93
           +   ++  VV+ +PW ++  +T F  V
Sbjct: 90  AVVGNYEAVVWDKPWMHFRNLTSFRKV 116

BLAST of Csa6G476020 vs. Swiss-Prot
Match: CYT8_ORYSJ (Cysteine proteinase inhibitor 8 OS=Oryza sativa subsp. japonica GN=Os03g0429000 PE=2 SV=1)

HSP 1 Score: 55.8 bits (133), Expect = 2.9e-07
Identity = 29/88 (32.95%), Postives = 45/88 (51.14%), Query Frame = 1

Query: 5   LGPYGPCDDSDAPYVKEIAERAVADHNKSAGTNYTLVSIVNCESVVVSGTNYRLVLSLKD 64
           +G + P  D   P+++E+   AV  H   +        + + E  VVSG NYRLV+S  D
Sbjct: 32  VGGWSPITDVGDPHIQELGGWAVERHASLSSDGLRFRRVTSGEQQVVSGMNYRLVVSASD 91

Query: 65  DS-TTSDFLVVVYYRPWDNYLEVTQFEP 92
            +  T+ ++ VVY + W N  ++T F+P
Sbjct: 92  PAGATASYVAVVYEQSWTNTRQLTSFKP 119

BLAST of Csa6G476020 vs. TrEMBL
Match: A0A0A0KKN2_CUCSA (Cysteine proteinase inhibitor OS=Cucumis sativus GN=Csa_6G476020 PE=3 SV=1)

HSP 1 Score: 197.6 bits (501), Expect = 6.9e-48
Identity = 94/94 (100.00%), Postives = 94/94 (100.00%), Query Frame = 1

Query: 1  MCTPLGPYGPCDDSDAPYVKEIAERAVADHNKSAGTNYTLVSIVNCESVVVSGTNYRLVL 60
          MCTPLGPYGPCDDSDAPYVKEIAERAVADHNKSAGTNYTLVSIVNCESVVVSGTNYRLVL
Sbjct: 1  MCTPLGPYGPCDDSDAPYVKEIAERAVADHNKSAGTNYTLVSIVNCESVVVSGTNYRLVL 60

Query: 61 SLKDDSTTSDFLVVVYYRPWDNYLEVTQFEPVTK 95
          SLKDDSTTSDFLVVVYYRPWDNYLEVTQFEPVTK
Sbjct: 61 SLKDDSTTSDFLVVVYYRPWDNYLEVTQFEPVTK 94

BLAST of Csa6G476020 vs. TrEMBL
Match: O80389_CUCSA (Cysteine proteinase inhibitor OS=Cucumis sativus PE=2 SV=1)

HSP 1 Score: 84.7 bits (208), Expect = 6.5e-14
Identity = 41/87 (47.13%), Postives = 62/87 (71.26%), Query Frame = 1

Query: 5  LGPYGPCDDSDAPYVKEIAERAVADHNKSAGTNYTLVSIVNCESVVVSGTNYRLVLSLKD 64
          +G Y PC D + P+VK+IAE AVA++NKS G + TLVSI+ CES VV+G N+RLVL  KD
Sbjct: 6  IGGYVPCKDPNDPHVKDIAEWAVAEYNKSQGHHLTLVSILKCESQVVAGVNWRLVLKCKD 65

Query: 65 DST-TSDFLVVVYYRPWDNYLEVTQFE 91
          ++    ++  VV+ + W+N+ ++  F+
Sbjct: 66 ENNGEGNYETVVWEKIWENFRQLITFD 92

BLAST of Csa6G476020 vs. TrEMBL
Match: I3SL29_LOTJA (Cysteine proteinase inhibitor OS=Lotus japonicus PE=2 SV=1)

HSP 1 Score: 82.4 bits (202), Expect = 3.2e-13
Identity = 37/88 (42.05%), Postives = 58/88 (65.91%), Query Frame = 1

Query: 5   LGPYGPCDDSDAPYVKEIAERAVADHNKSAGTNYTLVSIVNCESVVVSGTNYRLVLSLKD 64
           +G Y P  D +AP+V++IA+ A+ +HNK  G N  L  ++  +S VVSG NYRL L+  D
Sbjct: 22  VGGYSPIKDINAPHVRKIAQFAITEHNKHTGENLKLERVIKGDSQVVSGLNYRLTLAATD 81

Query: 65  DSTTSDFLVVVYYRPWDNYLEVTQFEPV 93
            ST+ ++LV+VY + W + +E+T F  +
Sbjct: 82  GSTSHNYLVIVYEQSWTHTIELTSFSRI 109

BLAST of Csa6G476020 vs. TrEMBL
Match: B9HCU3_POPTR (Cysteine proteinase inhibitor OS=Populus trichocarpa GN=POPTR_0006s01560g PE=3 SV=1)

HSP 1 Score: 81.3 bits (199), Expect = 7.2e-13
Identity = 44/87 (50.57%), Postives = 57/87 (65.52%), Query Frame = 1

Query: 5   LGPYGPCDDSDAPYVKEIAERAVADHNKSAGTNYTLVSIVNCESVVVSGTNYRLVLSLKD 64
           LG + P  D    +V EIAE AVA+HNK A +N  L SIV  ES VVSGTNYRLVL++K 
Sbjct: 25  LGGWSPIKDLKDKHVVEIAEFAVAEHNKEAKSNLMLESIVKGESQVVSGTNYRLVLAVKG 84

Query: 65  DSTTSDFLVVVYYRPWDNYLEVTQFEP 92
            +  + +  VVY +PW+N   +T F+P
Sbjct: 85  RANAT-YQAVVYEKPWENLKSLTSFQP 110

BLAST of Csa6G476020 vs. TrEMBL
Match: M0SNR0_MUSAM (Cysteine proteinase inhibitor OS=Musa acuminata subsp. malaccensis PE=3 SV=1)

HSP 1 Score: 80.9 bits (198), Expect = 9.4e-13
Identity = 41/89 (46.07%), Postives = 60/89 (67.42%), Query Frame = 1

Query: 5   LGPYGPCDDSDAPYVKEIAERAVADHNKSAGTNYTLVSIVNCESVVVSGTNYRLVLSLKD 64
           LG + P  ++D P+V+EIAE AVA+HNK A  +  L  +V  E+ VV+GTNYRLVL +KD
Sbjct: 32  LGGWTPIKNTDDPHVREIAEFAVAEHNKQANASLALSKVVKGETQVVAGTNYRLVLQVKD 91

Query: 65  DS-TTSDFLVVVYYRPWDNYLEVTQFEPV 93
            S     +  VV+ +PW+N+ ++T F+ V
Sbjct: 92  ASGARVKYQAVVWEKPWENFRQLTSFKLV 120

BLAST of Csa6G476020 vs. TAIR10
Match: AT5G47550.1 (AT5G47550.1 Cystatin/monellin superfamily protein)

HSP 1 Score: 65.5 bits (158), Expect = 2.1e-11
Identity = 32/88 (36.36%), Postives = 52/88 (59.09%), Query Frame = 1

Query: 5   LGPYGPCDDSDAPYVKEIAERAVADHNKSAGTNYTLVSIVNCESVVVSGTNYRLVLSLKD 64
           +G + P  +   P V EI E AV+++NK + +     ++V+ E+ VVSGTNYRL ++  D
Sbjct: 28  VGGWSPISNVTDPQVVEIGEFAVSEYNKRSESGLKFETVVSGETQVVSGTNYRLKVAAND 87

Query: 65  -DSTTSDFLVVVYYRPWDNYLEVTQFEP 92
            D  + ++L +V+ +PW  +  +T FEP
Sbjct: 88  GDGVSKNYLAIVWDKPWMKFRNLTSFEP 115

BLAST of Csa6G476020 vs. NCBI nr
Match: gi|700193107|gb|KGN48311.1| (hypothetical protein Csa_6G476020 [Cucumis sativus])

HSP 1 Score: 197.6 bits (501), Expect = 9.9e-48
Identity = 94/94 (100.00%), Postives = 94/94 (100.00%), Query Frame = 1

Query: 1  MCTPLGPYGPCDDSDAPYVKEIAERAVADHNKSAGTNYTLVSIVNCESVVVSGTNYRLVL 60
          MCTPLGPYGPCDDSDAPYVKEIAERAVADHNKSAGTNYTLVSIVNCESVVVSGTNYRLVL
Sbjct: 1  MCTPLGPYGPCDDSDAPYVKEIAERAVADHNKSAGTNYTLVSIVNCESVVVSGTNYRLVL 60

Query: 61 SLKDDSTTSDFLVVVYYRPWDNYLEVTQFEPVTK 95
          SLKDDSTTSDFLVVVYYRPWDNYLEVTQFEPVTK
Sbjct: 61 SLKDDSTTSDFLVVVYYRPWDNYLEVTQFEPVTK 94

BLAST of Csa6G476020 vs. NCBI nr
Match: gi|525507274|ref|NP_001267677.1| (cysteine proteinase inhibitor 5-like [Cucumis sativus])

HSP 1 Score: 84.7 bits (208), Expect = 9.3e-14
Identity = 41/87 (47.13%), Postives = 62/87 (71.26%), Query Frame = 1

Query: 5  LGPYGPCDDSDAPYVKEIAERAVADHNKSAGTNYTLVSIVNCESVVVSGTNYRLVLSLKD 64
          +G Y PC D + P+VK+IAE AVA++NKS G + TLVSI+ CES VV+G N+RLVL  KD
Sbjct: 6  IGGYVPCKDPNDPHVKDIAEWAVAEYNKSQGHHLTLVSILKCESQVVAGVNWRLVLKCKD 65

Query: 65 DST-TSDFLVVVYYRPWDNYLEVTQFE 91
          ++    ++  VV+ + W+N+ ++  F+
Sbjct: 66 ENNGEGNYETVVWEKIWENFRQLITFD 92

BLAST of Csa6G476020 vs. NCBI nr
Match: gi|388505810|gb|AFK40971.1| (unknown [Lotus japonicus])

HSP 1 Score: 82.4 bits (202), Expect = 4.6e-13
Identity = 37/88 (42.05%), Postives = 58/88 (65.91%), Query Frame = 1

Query: 5   LGPYGPCDDSDAPYVKEIAERAVADHNKSAGTNYTLVSIVNCESVVVSGTNYRLVLSLKD 64
           +G Y P  D +AP+V++IA+ A+ +HNK  G N  L  ++  +S VVSG NYRL L+  D
Sbjct: 22  VGGYSPIKDINAPHVRKIAQFAITEHNKHTGENLKLERVIKGDSQVVSGLNYRLTLAATD 81

Query: 65  DSTTSDFLVVVYYRPWDNYLEVTQFEPV 93
            ST+ ++LV+VY + W + +E+T F  +
Sbjct: 82  GSTSHNYLVIVYEQSWTHTIELTSFSRI 109

BLAST of Csa6G476020 vs. NCBI nr
Match: gi|743929484|ref|XP_011008977.1| (PREDICTED: cysteine proteinase inhibitor 5-like [Populus euphratica])

HSP 1 Score: 82.0 bits (201), Expect = 6.0e-13
Identity = 44/87 (50.57%), Postives = 59/87 (67.82%), Query Frame = 1

Query: 5   LGPYGPCDDSDAPYVKEIAERAVADHNKSAGTNYTLVSIVNCESVVVSGTNYRLVLSLKD 64
           LG + P  D    +V EIAE AVA++NK A +N TLVSIV  ES VVSGTNYRLVL++K 
Sbjct: 25  LGGWSPIKDLKDKHVVEIAEFAVAEYNKEAKSNLTLVSIVKGESQVVSGTNYRLVLAVK- 84

Query: 65  DSTTSDFLVVVYYRPWDNYLEVTQFEP 92
              ++ +  VVY +PW++   +T F+P
Sbjct: 85  GGASAKYQAVVYEKPWEHLKSLTSFQP 110

BLAST of Csa6G476020 vs. NCBI nr
Match: gi|224086460|ref|XP_002307893.1| (cystatin family protein [Populus trichocarpa])

HSP 1 Score: 81.3 bits (199), Expect = 1.0e-12
Identity = 44/87 (50.57%), Postives = 57/87 (65.52%), Query Frame = 1

Query: 5   LGPYGPCDDSDAPYVKEIAERAVADHNKSAGTNYTLVSIVNCESVVVSGTNYRLVLSLKD 64
           LG + P  D    +V EIAE AVA+HNK A +N  L SIV  ES VVSGTNYRLVL++K 
Sbjct: 25  LGGWSPIKDLKDKHVVEIAEFAVAEHNKEAKSNLMLESIVKGESQVVSGTNYRLVLAVKG 84

Query: 65  DSTTSDFLVVVYYRPWDNYLEVTQFEP 92
            +  + +  VVY +PW+N   +T F+P
Sbjct: 85  RANAT-YQAVVYEKPWENLKSLTSFQP 110

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
CYT5_ARATH3.7e-1036.36Cysteine proteinase inhibitor 5 OS=Arabidopsis thaliana GN=CYS5 PE=2 SV=2[more]
CYT1_ACTDE6.3e-1034.48Cysteine proteinase inhibitor 1 OS=Actinidia deliciosa PE=1 SV=1[more]
CYT8_ORYSJ2.9e-0732.95Cysteine proteinase inhibitor 8 OS=Oryza sativa subsp. japonica GN=Os03g0429000 ... [more]
Match NameE-valueIdentityDescription
A0A0A0KKN2_CUCSA6.9e-48100.00Cysteine proteinase inhibitor OS=Cucumis sativus GN=Csa_6G476020 PE=3 SV=1[more]
O80389_CUCSA6.5e-1447.13Cysteine proteinase inhibitor OS=Cucumis sativus PE=2 SV=1[more]
I3SL29_LOTJA3.2e-1342.05Cysteine proteinase inhibitor OS=Lotus japonicus PE=2 SV=1[more]
B9HCU3_POPTR7.2e-1350.57Cysteine proteinase inhibitor OS=Populus trichocarpa GN=POPTR_0006s01560g PE=3 S... [more]
M0SNR0_MUSAM9.4e-1346.07Cysteine proteinase inhibitor OS=Musa acuminata subsp. malaccensis PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT5G47550.12.1e-1136.36 Cystatin/monellin superfamily protein[more]
Match NameE-valueIdentityDescription
gi|700193107|gb|KGN48311.1|9.9e-48100.00hypothetical protein Csa_6G476020 [Cucumis sativus][more]
gi|525507274|ref|NP_001267677.1|9.3e-1447.13cysteine proteinase inhibitor 5-like [Cucumis sativus][more]
gi|388505810|gb|AFK40971.1|4.6e-1342.05unknown [Lotus japonicus][more]
gi|743929484|ref|XP_011008977.1|6.0e-1350.57PREDICTED: cysteine proteinase inhibitor 5-like [Populus euphratica][more]
gi|224086460|ref|XP_002307893.1|1.0e-1250.57cystatin family protein [Populus trichocarpa][more]
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR000010Cystatin_dom
IPR020381Proteinase inhibitor I25, cystatin, conserved region
IPR027214Cystatin
Vocabulary: Molecular Function
TermDefinition
GO:0004869cysteine-type endopeptidase inhibitor activity
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0010951 negative regulation of endopeptidase activity
biological_process GO:2000117 negative regulation of cysteine-type endopeptidase activity
cellular_component GO:0005575 cellular_component
molecular_function GO:0004869 cysteine-type endopeptidase inhibitor activity
molecular_function GO:0002020 protease binding
This gene is associated with the following unigenes:
Unigene NameAnalysis NameSequence type in Unigene
CU122975cucumber EST collection version 3.0transcribed_cluster
CU125714cucumber EST collection version 3.0transcribed_cluster

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Csa6G476020.1Csa6G476020.1mRNA


The following transcribed_cluster feature(s) are associated with this gene:

Feature NameUnique NameType
CU122975CU122975transcribed_cluster
CU125714CU125714transcribed_cluster


Analysis Name: InterPro Annotations of cucumber (Chinese Long)
Date Performed: 2016-09-28
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000010Cystatin domainSMARTSM00043CY_4coord: 5..92
score: 2.
IPR020381Proteinase inhibitor I25, cystatin, conserved regionPRODOMPD001231coord: 18..90
score: 2.0
IPR020381Proteinase inhibitor I25, cystatin, conserved regionPFAMPF16845SQAPIcoord: 13..91
score: 2.5
IPR027214CystatinPANTHERPTHR11413CYSTATIN FAMILY MEMBERcoord: 5..92
score: 2.0
NoneNo IPR availableGENE3DG3DSA:3.10.450.10coord: 5..91
score: 1.5
NoneNo IPR availableunknownSSF54403Cystatin/monellincoord: 4..91
score: 2.92