CsGy5G027910 (gene) Cucumber (Gy14) v2 | Cucurbit Genomics Database (CuGenDB)

Overview


Name	CsGy5G027910
Type	gene
Organism	Cucumis sativus (Cucumber (Gy14) v2)
Description	phytolongin Phyl2.1-like
Location	Chr5 : 31666334 .. 31667111 (-)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSexon

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGTTTCCAATCCAGATCTCGTCTTATATTCCTGCATTGCAAGAGGAACTATAATCCTCGCTGAATTCACAAGAGAACCAAATCTCGATGACTTACCTTTCAGATGTATCGAGAAAACTCCTCCTCATCACTCCATCTTCTCTCACACCATTAGAAACAGAGCATACACCTTTTTGATCGAAGGTTCTTTTGTTTATTTCGGAATCTTCCATGAAGACCTTAACAAATCCGAGGTTTCTGGTTTCTTAAACCGCTTAAAGCGTGATTTCGAGGTGTTTCTCGAAAGCGAATCGCTCAAGGGTTTTGAAAACTTCACTTCTCACTGTTTTCAATCGCACTTCGATTCGAGTTTCCGGCATCTCCTGGGGTTGAATCTGAACAAAACATCGTCCAACGGATTGAAGGGAAGTCAGAGATCGAGCTTGGACTCAAGTTTAAGAAAGCAAATACCATTGGTTTCCAAGTTTTCAAATCCGGGTAGCTTAATGAAGAAGAAGAAGAAGGTGACTGGGGATGGTAATTGGGATGGAGGGAAGTATGGAATGGATGATTCTGTGGATCTGTATGATGATAACAATGGTTTATGCAGTAGAGATATTACATTGCTGATGCAGAAGCATGGTTCTCATAATAGTGAGCGTCAAAAAGCAAAGCAAGTATGGAGAAAACATGTATGGGTTGTTTTATCCATTGATTTGTTTGTATGTTTGGTTTTGTTTGGCATATGGCTTTGGGTTTGCAGGGGATTTCAGTGCATTGAAAGCTGAAAATGCCTGA

mRNA sequence

ATGGTTTCCAATCCAGATCTCGTCTTATATTCCTGCATTGCAAGAGGAACTATAATCCTCGCTGAATTCACAAGAGAACCAAATCTCGATGACTTACCTTTCAGATGTATCGAGAAAACTCCTCCTCATCACTCCATCTTCTCTCACACCATTAGAAACAGAGCATACACCTTTTTGATCGAAGGTTCTTTTGTTTATTTCGGAATCTTCCATGAAGACCTTAACAAATCCGAGGTTTCTGGTTTCTTAAACCGCTTAAAGCGTGATTTCGAGGTGTTTCTCGAAAGCGAATCGCTCAAGGGTTTTGAAAACTTCACTTCTCACTGTTTTCAATCGCACTTCGATTCGAGTTTCCGGCATCTCCTGGGGTTGAATCTGAACAAAACATCGTCCAACGGATTGAAGGGAAGTCAGAGATCGAGCTTGGACTCAAGTTTAAGAAAGCAAATACCATTGGTTTCCAAGTTTTCAAATCCGGGTAGCTTAATGAAGAAGAAGAAGAAGGTGACTGGGGATGGTAATTGGGATGGAGGGAAGTATGGAATGGATGATTCTGTGGATCTGTATGATGATAACAATGGTTTATGCAGTAGAGATATTACATTGCTGATGCAGAAGCATGGGGATTTCAGTGCATTGAAAGCTGAAAATGCCTGA

Coding sequence (CDS)

ATGGTTTCCAATCCAGATCTCGTCTTATATTCCTGCATTGCAAGAGGAACTATAATCCTCGCTGAATTCACAAGAGAACCAAATCTCGATGACTTACCTTTCAGATGTATCGAGAAAACTCCTCCTCATCACTCCATCTTCTCTCACACCATTAGAAACAGAGCATACACCTTTTTGATCGAAGGTTCTTTTGTTTATTTCGGAATCTTCCATGAAGACCTTAACAAATCCGAGGTTTCTGGTTTCTTAAACCGCTTAAAGCGTGATTTCGAGGTGTTTCTCGAAAGCGAATCGCTCAAGGGTTTTGAAAACTTCACTTCTCACTGTTTTCAATCGCACTTCGATTCGAGTTTCCGGCATCTCCTGGGGTTGAATCTGAACAAAACATCGTCCAACGGATTGAAGGGAAGTCAGAGATCGAGCTTGGACTCAAGTTTAAGAAAGCAAATACCATTGGTTTCCAAGTTTTCAAATCCGGGTAGCTTAATGAAGAAGAAGAAGAAGGTGACTGGGGATGGTAATTGGGATGGAGGGAAGTATGGAATGGATGATTCTGTGGATCTGTATGATGATAACAATGGTTTATGCAGTAGAGATATTACATTGCTGATGCAGAAGCATGGGGATTTCAGTGCATTGAAAGCTGAAAATGCCTGA

Protein sequence

MVSNPDLVLYSCIARGTIILAEFTREPNLDDLPFRCIEKTPPHHSIFSHTIRNRAYTFLIEGSFVYFGIFHEDLNKSEVSGFLNRLKRDFEVFLESESLKGFENFTSHCFQSHFDSSFRHLLGLNLNKTSSNGLKGSQRSSLDSSLRKQIPLVSKFSNPGSLMKKKKKVTGDGNWDGGKYGMDDSVDLYDDNNGLCSRDITLLMQKHGDFSALKAENA

Homology

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BLAST of CsGy5G027910 vs. NCBI nr
Match: KGN52421.1 (hypothetical protein Csa_5G633190 [Cucumis sativus])

HSP 1 Score: 435.3 bits (1118), Expect = 1.3e-118
Identity = 218/222 (98.20%), Postives = 218/222 (98.20%), Query Frame = 0


Query: 1   MVSNPDLVLYSCIARGTIILAEFTREPNLDDLPFRCIEKTPPHHSIFSHTIRNRAYTFLI 60
           MVSNPDLVLYSCIARGTIILAEFTREPNLDDLPFRCIEKTPPHHSIFSHTIRNRAYTFLI
Sbjct: 1   MVSNPDLVLYSCIARGTIILAEFTREPNLDDLPFRCIEKTPPHHSIFSHTIRNRAYTFLI 60

Query: 61  EGSFVYFGIFHEDLNKSEVSGFLNRLKRDFEVFLESESLKGFENFTSHCFQSHFDSSFRH 120
           EGSFVYFGIFHEDLNKSEVSGFLNRLKRDFEVFLESESLKGFENFTSHCFQSHFDSSFRH
Sbjct: 61  EGSFVYFGIFHEDLNKSEVSGFLNRLKRDFEVFLESESLKGFENFTSHCFQSHFDSSFRH 120

Query: 121 LLGLNLNKTSSNGLKGSQRSSLDSSLRKQIPLVSKFSNPGSLMKKKKKVTGDGNWDGGKY 180
           LLGLNLNKTSSNGLKGSQRSSLDSSLRKQIPLVSKFSNPGSLMKKKKKVTGDGNWDGGKY
Sbjct: 121 LLGLNLNKTSSNGLKGSQRSSLDSSLRKQIPLVSKFSNPGSLMKKKKKVTGDGNWDGGKY 180

Query: 181 GMDDSVDLYDDNNGLCSRDITLLMQKHG----DFSALKAENA 219
           GMDDSVDLYDDNNGLCSRDITLLMQKHG    DFSALKAENA
Sbjct: 181 GMDDSVDLYDDNNGLCSRDITLLMQKHGSHNRDFSALKAENA 222

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BLAST of CsGy5G027910 vs. NCBI nr
Match: XP_004148677.1 (PREDICTED: probable VAMP-like protein At1g33475 [Cucumis sativus])

HSP 1 Score: 422.5 bits (1085), Expect = 8.4e-115
Identity = 208/212 (98.11%), Postives = 210/212 (99.06%), Query Frame = 0


Query: 1   MVSNPDLVLYSCIARGTIILAEFTREPNLDDLPFRCIEKTPPHHSIFSHTIRNRAYTFLI 60
           MVSNPDLVLYSCIARGTIILAEFTREPNLDDLPFRCIEKTPPHHSIFSHTIRNRAYTFLI
Sbjct: 1   MVSNPDLVLYSCIARGTIILAEFTREPNLDDLPFRCIEKTPPHHSIFSHTIRNRAYTFLI 60

Query: 61  EGSFVYFGIFHEDLNKSEVSGFLNRLKRDFEVFLESESLKGFENFTSHCFQSHFDSSFRH 120
           EGSFVYFGIFHEDLNKSEVSGFLNRLKRDFEVFLESESLKGFENFTSHCFQSHFDSSFRH
Sbjct: 61  EGSFVYFGIFHEDLNKSEVSGFLNRLKRDFEVFLESESLKGFENFTSHCFQSHFDSSFRH 120

Query: 121 LLGLNLNKTSSNGLKGSQRSSLDSSLRKQIPLVSKFSNPGSLMKKKKKVTGDGNWDGGKY 180
           LLGLNLNKTSSNGLKGSQRSSLDSSLRKQIPLVSKFSNPGSLMKKKKKVTGDGNWDGGKY
Sbjct: 121 LLGLNLNKTSSNGLKGSQRSSLDSSLRKQIPLVSKFSNPGSLMKKKKKVTGDGNWDGGKY 180

Query: 181 GMDDSVDLYDDNNGLCSRDITLLMQKHGDFSA 213
           GMDDSVDLYDDNNGLCSRDITLLMQKHG  ++
Sbjct: 181 GMDDSVDLYDDNNGLCSRDITLLMQKHGSHNS 212

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BLAST of CsGy5G027910 vs. NCBI nr
Match: XP_008458710.1 (PREDICTED: phytolongin Phyl2.1-like [Cucumis melo])

HSP 1 Score: 402.9 bits (1034), Expect = 6.9e-109
Identity = 199/212 (93.87%), Postives = 204/212 (96.23%), Query Frame = 0


Query: 1   MVSNPDLVLYSCIARGTIILAEFTREPNLDDLPFRCIEKTPPHHSIFSHTIRNRAYTFLI 60
           MVSNPDLV YSCIARGTIILAEFTREPNLDDLP RCIEK PPHHSIFSHTIRNRAYTFLI
Sbjct: 1   MVSNPDLVFYSCIARGTIILAEFTREPNLDDLPLRCIEKAPPHHSIFSHTIRNRAYTFLI 60

Query: 61  EGSFVYFGIFHEDLNKSEVSGFLNRLKRDFEVFLESESLKGFENFTSHCFQSHFDSSFRH 120
           EGSFVYFGIFHEDLNKSEVSGFLNRLKRDFEVFLESESLKGFENFTS+CFQS FDSSFRH
Sbjct: 61  EGSFVYFGIFHEDLNKSEVSGFLNRLKRDFEVFLESESLKGFENFTSYCFQSQFDSSFRH 120

Query: 121 LLGLNLNKTSSNGLKGSQRSSLDSSLRKQIPLVSKFSNPGSLMKKKKKVTGDGNWDGGKY 180
           LLGLNLNKTSSNGL GSQRSSLDSS RKQIPLVSKFSNPGSLMKKKKKVTGDGNW+GGKY
Sbjct: 121 LLGLNLNKTSSNGLMGSQRSSLDSSTRKQIPLVSKFSNPGSLMKKKKKVTGDGNWEGGKY 180

Query: 181 GMDDSVDLYDDNNGLCSRDITLLMQKHGDFSA 213
           GMDDSVDLYDDNNGLCSRDITLLMQK+G  ++
Sbjct: 181 GMDDSVDLYDDNNGLCSRDITLLMQKNGSHNS 212

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BLAST of CsGy5G027910 vs. NCBI nr
Match: XP_022938537.1 (phytolongin Phyl2.2-like [Cucurbita moschata])

HSP 1 Score: 305.8 bits (782), Expect = 1.1e-79
Identity = 158/209 (75.60%), Postives = 170/209 (81.34%), Query Frame = 0


Query: 1   MVSNPDLVLYSCIARGTIILAEFTREPNLDDLPFRCIEKTPPHHSIFSHTIRNRAYTFLI 60
           MVSNPDLVLY+CIA GT+ILAEFTRE NLDDL FRCIEK PPHHSIFSHT+R R Y FLI
Sbjct: 1   MVSNPDLVLYACIASGTVILAEFTRELNLDDLAFRCIEKAPPHHSIFSHTVRKRVYMFLI 60

Query: 61  EGSFVYFGIFHEDLNKSEVSGFLNRLKRDFEVFLESESLKGFENFTSHCFQSHFDSSFRH 120
           EG FVYFGIFHEDL KSE+ GFLNRL   FE FLESES KGFENFTS+CFQS FDS+FRH
Sbjct: 61  EGPFVYFGIFHEDLYKSEICGFLNRLSCVFEEFLESESFKGFENFTSYCFQSQFDSTFRH 120

Query: 121 LLGLNLNKTSSNGLKGSQRSSLDSSLRKQIPLVSKFSNPGSLM-KKKKKVTGDGNWDGGK 180
           LLGLNLN TS + LK +QRSSLDSS RKQIPL+SKFSNP SLM      V GD N + GK
Sbjct: 121 LLGLNLNNTSYSVLKEAQRSSLDSSKRKQIPLISKFSNPSSLMXXXXXXVVGDANEEAGK 180

Query: 181 YGMDDSVDLYDDNNGLCSRDITLLMQKHG 209
           Y M DS DL D NNGLCSRD +LL QK+G
Sbjct: 181 YAMVDSADLNDHNNGLCSRDSSLLKQKNG 209

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BLAST of CsGy5G027910 vs. NCBI nr
Match: XP_022133479.1 (phytolongin Phyl2.2-like [Momordica charantia])

HSP 1 Score: 298.1 bits (762), Expect = 2.4e-77
Identity = 154/215 (71.63%), Postives = 173/215 (80.47%), Query Frame = 0


Query: 1   MVSNPDLVLYSCIARGTIILAEFTREPNLDDLPFRCIEKTPPHHSIFSHTIRNRAYTFLI 60
           MVSNPDLV Y+CIARGT+ILAEFTRE NLDDL FRCIEK PP+HS FSHT+RNRAY FLI
Sbjct: 1   MVSNPDLVFYACIARGTVILAEFTRESNLDDLAFRCIEKAPPNHSTFSHTVRNRAYAFLI 60

Query: 61  EGSFVYFGIFHEDLNKSEVSGFLNRLKRDFEVFLESESLKGFENFTSHCFQSHFDSSFRH 120
           E SFVYFGIFHEDL KSEVSGFLNR+KR F  F+ESE L GF+NFT +CFQ+ FDS FR 
Sbjct: 61  EESFVYFGIFHEDLYKSEVSGFLNRVKRVFTDFIESELLNGFDNFTPYCFQAQFDSIFRQ 120

Query: 121 LLGLNLNKTSS-NGLKGSQRSSLDSSLRKQIPLVSKFSNPGSLMKKKKKVTGDGNWDGGK 180
           +LG NLN + + +   G  R S+DSS+RKQIP +SKFSNP +LMKKKKKV GD N +GGK
Sbjct: 121 ILGSNLNTSGNLSTSSGVLRPSVDSSIRKQIPFISKFSNPSNLMKKKKKVVGDANGEGGK 180

Query: 181 YG--MDDSVDLYDDNNGLCSRDITLLMQKHGDFSA 213
           Y   MDDS D YDD NGL SRDITLLMQK+G   A
Sbjct: 181 YATTMDDSADPYDDMNGLGSRDITLLMQKNGSHIA 215

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BLAST of CsGy5G027910 vs. TAIR10
Match: AT5G52990.1 (SNARE-like superfamily protein)

HSP 1 Score: 120.6 bits (301), Expect = 1.2e-27
Identity = 77/219 (35.16%), Postives = 116/219 (52.97%), Query Frame = 0


Query: 1   MVSNPDLVLYSCIARGTIILAEFT--REPNLDDLPFRCIEKTPPHHSIFSHTIRNRAYTF 60
           M+SNP L+ Y+CIA+GT++LAEF   +EP ++ +  RCIE TPPHHS+FSHT+  + YTF
Sbjct: 1   MISNPSLLSYTCIAKGTVVLAEFVSRQEPGIEAIALRCIENTPPHHSMFSHTVHKKTYTF 60

Query: 61  LI-EGSFVYFGIFHEDLNKSEVSGFLNRLKRDFEVFLE--SESLKGFENFTSHCFQSHFD 120
            I + SFVYF I  E + K E    LNRL+   E  ++     ++   N  SHC Q   D
Sbjct: 61  AIDDDSFVYFSISDESMEKPESFWVLNRLRSAIEDLIKDGGSDVETLINPVSHCLQLKLD 120

Query: 121 SSFRHLLGLNLNKTSSNGLKGSQRS--------SLDSSLRKQIPLVSKFSNPGSLMKKKK 180
             F  ++G+   +     L GS RS        S+DSS  ++  L+     P   +KKKK
Sbjct: 121 PVFAEIVGVVDLELLDMDLVGSPRSVARESRNPSIDSSKGRRAALMPLLGKPLKALKKKK 180

Query: 181 KVTGDGNWDGGKYGMDDSVDLYDDNNGLCSRDITLLMQK 207
           ++  +    G    +    ++ + N  LC      +++K
Sbjct: 181 RLHNEAK-GGDSCEVGSIQEISEKNVDLCGNGNNGVLRK 218

[Close]

BLAST of CsGy5G027910 vs. TAIR10
Match: AT4G27840.1 (SNARE-like superfamily protein)

HSP 1 Score: 118.6 bits (296), Expect = 4.7e-27
Identity = 79/198 (39.90%), Postives = 103/198 (52.02%), Query Frame = 0


Query: 1   MVSNPDLVLYSCIARGTIILAEFT--REPNLDDLPFRCIEKTPPHHSIFSHTIRNRAYTF 60
           M SN  ++ YSCIA+GT+ILAEF    EP ++DL  RCIE  PPHHS+ SHT+  R Y  
Sbjct: 1   MTSNQRMLFYSCIAKGTVILAEFASKEEPGIEDLALRCIENVPPHHSMISHTVHKRTYAL 60

Query: 61  LIEGSFVYFGIFHEDLNKSEVSGFLNRLKRDFEVFLESES-LKGFENFTSHCFQSHFDSS 120
           +I+G F YF I  E + KSE     NRLK   E  +E  S     +N T HC QS  D  
Sbjct: 61  IIDGLFSYFAILDEVVAKSESVWLFNRLKSATESLMEDGSTADSLDNPTQHCLQSKLDPV 120

Query: 121 FRHLLGLNLNKTSSNGLK-GSQRS----------SLDSSL-RKQIPLVSKFSNPGSLMKK 180
           F  +  +  N      L+ GS RS          SLDSS  RK   L+     P  ++K 
Sbjct: 121 FAEIAAIGGNHNKDLELEFGSPRSIAREIKSNNQSLDSSKGRKGGALMPLLGKPLRVLKN 180

[Close]

BLAST of CsGy5G027910 vs. TAIR10
Match: AT4G10170.1 (SNARE-like superfamily protein)

HSP 1 Score: 59.3 bits (142), Expect = 3.4e-09
Identity = 34/96 (35.42%), Postives = 48/96 (50.00%), Query Frame = 0


Query: 8   VLYSCIARGTIILAEFT-REPNLDDLPFRCIEKTPPHHSIFSHTIRNRAYTFLIEGSFVY 67
           V Y C++R   IL  +   +   + L   C+EK+PP H+ +  TI  R + FLI   FVY
Sbjct: 8   VHYCCVSRDNQILYSYNGGDQTNESLAALCLEKSPPFHTWYFETIGKRRFGFLIGDGFVY 67

Query: 68  FGIFHEDLNKSEVSGFLNRLKRDFEVFLESESLKGF 103
           F I  E L +S V  FL  L+ +F+      S   F
Sbjct: 68  FAIVDEVLKRSSVLKFLEHLRDEFKKAARENSRGSF 103

[Close]

BLAST of CsGy5G027910 vs. TAIR10
Match: AT2G32670.1 (vesicle-associated membrane protein 725)

HSP 1 Score: 57.4 bits (137), Expect = 1.3e-08
Identity = 26/84 (30.95%), Postives = 47/84 (55.95%), Query Frame = 0


Query: 8   VLYSCIARGTIILAEFTR-EPNLDDLPFRCIEKTPPHHSIFSHTIRNRAYTFLIEGSFVY 67
           ++YS +ARGT+IL E+T  + N   +  +C++K P  ++ F++      + +L+E  F Y
Sbjct: 71  LIYSFVARGTVILVEYTEFKGNFTAVAAQCLQKLPSSNNKFTYNCDGHTFNYLVENGFTY 130

Query: 68  FGIFHEDLNKSEVSGFLNRLKRDF 91
             +  E + +     FL R+K DF
Sbjct: 131 CVVAVESVGRQIPMAFLERVKEDF 154

[Close]

BLAST of CsGy5G027910 vs. TAIR10
Match: AT1G04760.1 (vesicle-associated membrane protein 726)

HSP 1 Score: 56.6 bits (135), Expect = 2.2e-08
Identity = 26/84 (30.95%), Postives = 46/84 (54.76%), Query Frame = 0


Query: 8  VLYSCIARGTIILAEFTR-EPNLDDLPFRCIEKTPPHHSIFSHTIRNRAYTFLIEGSFVY 67
          ++YS +ARGT+ILAE+T  + N   +  +C++K P  ++ F++      + +L +  F Y
Sbjct: 6  LIYSFVARGTVILAEYTEFKGNFTSVAAQCLQKLPSSNNKFTYNCDGHTFNYLADNGFTY 65

Query: 68 FGIFHEDLNKSEVSGFLNRLKRDF 91
            +  E   +     FL R+K DF
Sbjct: 66 CVVVIESAGRQIPMAFLERVKEDF 89

[Close]

BLAST of CsGy5G027910 vs. Swiss-Prot
Match: sp|Q9LVV3|PHL22_ARATH (Phytolongin Phyl2.2 OS=Arabidopsis thaliana OX=3702 GN=PHYL2.2 PE=2 SV=1)

HSP 1 Score: 120.6 bits (301), Expect = 2.2e-26
Identity = 77/219 (35.16%), Postives = 116/219 (52.97%), Query Frame = 0


Query: 1   MVSNPDLVLYSCIARGTIILAEFT--REPNLDDLPFRCIEKTPPHHSIFSHTIRNRAYTF 60
           M+SNP L+ Y+CIA+GT++LAEF   +EP ++ +  RCIE TPPHHS+FSHT+  + YTF
Sbjct: 1   MISNPSLLSYTCIAKGTVVLAEFVSRQEPGIEAIALRCIENTPPHHSMFSHTVHKKTYTF 60

Query: 61  LI-EGSFVYFGIFHEDLNKSEVSGFLNRLKRDFEVFLE--SESLKGFENFTSHCFQSHFD 120
            I + SFVYF I  E + K E    LNRL+   E  ++     ++   N  SHC Q   D
Sbjct: 61  AIDDDSFVYFSISDESMEKPESFWVLNRLRSAIEDLIKDGGSDVETLINPVSHCLQLKLD 120

Query: 121 SSFRHLLGLNLNKTSSNGLKGSQRS--------SLDSSLRKQIPLVSKFSNPGSLMKKKK 180
             F  ++G+   +     L GS RS        S+DSS  ++  L+     P   +KKKK
Sbjct: 121 PVFAEIVGVVDLELLDMDLVGSPRSVARESRNPSIDSSKGRRAALMPLLGKPLKALKKKK 180

Query: 181 KVTGDGNWDGGKYGMDDSVDLYDDNNGLCSRDITLLMQK 207
           ++  +    G    +    ++ + N  LC      +++K
Sbjct: 181 RLHNEAK-GGDSCEVGSIQEISEKNVDLCGNGNNGVLRK 218

[Close]

BLAST of CsGy5G027910 vs. Swiss-Prot
Match: sp|Q9STP2|PHL21_ARATH (Phytolongin Phyl2.1 OS=Arabidopsis thaliana OX=3702 GN=PHYL2.1 PE=2 SV=1)

HSP 1 Score: 118.6 bits (296), Expect = 8.5e-26
Identity = 79/198 (39.90%), Postives = 103/198 (52.02%), Query Frame = 0


Query: 1   MVSNPDLVLYSCIARGTIILAEFT--REPNLDDLPFRCIEKTPPHHSIFSHTIRNRAYTF 60
           M SN  ++ YSCIA+GT+ILAEF    EP ++DL  RCIE  PPHHS+ SHT+  R Y  
Sbjct: 1   MTSNQRMLFYSCIAKGTVILAEFASKEEPGIEDLALRCIENVPPHHSMISHTVHKRTYAL 60

Query: 61  LIEGSFVYFGIFHEDLNKSEVSGFLNRLKRDFEVFLESES-LKGFENFTSHCFQSHFDSS 120
           +I+G F YF I  E + KSE     NRLK   E  +E  S     +N T HC QS  D  
Sbjct: 61  IIDGLFSYFAILDEVVAKSESVWLFNRLKSATESLMEDGSTADSLDNPTQHCLQSKLDPV 120

Query: 121 FRHLLGLNLNKTSSNGLK-GSQRS----------SLDSSL-RKQIPLVSKFSNPGSLMKK 180
           F  +  +  N      L+ GS RS          SLDSS  RK   L+     P  ++K 
Sbjct: 121 FAEIAAIGGNHNKDLELEFGSPRSIAREIKSNNQSLDSSKGRKGGALMPLLGKPLRVLKN 180

[Close]

BLAST of CsGy5G027910 vs. Swiss-Prot
Match: sp|Q9SN26|PHL11_ARATH (Phytolongin Phyl1.1 OS=Arabidopsis thaliana OX=3702 GN=PHYL1.1 PE=2 SV=1)

HSP 1 Score: 59.3 bits (142), Expect = 6.1e-08
Identity = 34/96 (35.42%), Postives = 48/96 (50.00%), Query Frame = 0


Query: 8   VLYSCIARGTIILAEFT-REPNLDDLPFRCIEKTPPHHSIFSHTIRNRAYTFLIEGSFVY 67
           V Y C++R   IL  +   +   + L   C+EK+PP H+ +  TI  R + FLI   FVY
Sbjct: 8   VHYCCVSRDNQILYSYNGGDQTNESLAALCLEKSPPFHTWYFETIGKRRFGFLIGDGFVY 67

Query: 68  FGIFHEDLNKSEVSGFLNRLKRDFEVFLESESLKGF 103
           F I  E L +S V  FL  L+ +F+      S   F
Sbjct: 68  FAIVDEVLKRSSVLKFLEHLRDEFKKAARENSRGSF 103

[Close]

BLAST of CsGy5G027910 vs. Swiss-Prot
Match: sp|O48850|VA725_ARATH (Vesicle-associated membrane protein 725 OS=Arabidopsis thaliana OX=3702 GN=VAMP725 PE=2 SV=2)

HSP 1 Score: 57.4 bits (137), Expect = 2.3e-07
Identity = 26/84 (30.95%), Postives = 47/84 (55.95%), Query Frame = 0


Query: 8   VLYSCIARGTIILAEFTR-EPNLDDLPFRCIEKTPPHHSIFSHTIRNRAYTFLIEGSFVY 67
           ++YS +ARGT+IL E+T  + N   +  +C++K P  ++ F++      + +L+E  F Y
Sbjct: 71  LIYSFVARGTVILVEYTEFKGNFTAVAAQCLQKLPSSNNKFTYNCDGHTFNYLVENGFTY 130

Query: 68  FGIFHEDLNKSEVSGFLNRLKRDF 91
             +  E + +     FL R+K DF
Sbjct: 131 CVVAVESVGRQIPMAFLERVKEDF 154

[Close]

BLAST of CsGy5G027910 vs. Swiss-Prot
Match: sp|P47192|VA722_ARATH (Vesicle-associated membrane protein 722 OS=Arabidopsis thaliana OX=3702 GN=VAMP722 PE=2 SV=2)

HSP 1 Score: 57.0 bits (136), Expect = 3.0e-07
Identity = 26/84 (30.95%), Postives = 46/84 (54.76%), Query Frame = 0


Query: 8  VLYSCIARGTIILAEFTR-EPNLDDLPFRCIEKTPPHHSIFSHTIRNRAYTFLIEGSFVY 67
          ++YS +ARGT+IL EFT  + N   +  +C++K P  ++ F++      + +L+E  F Y
Sbjct: 6  LIYSFVARGTVILVEFTDFKGNFTSIAAQCLQKLPSSNNKFTYNCDGHTFNYLVENGFTY 65

Query: 68 FGIFHEDLNKSEVSGFLNRLKRDF 91
            +  +   +     FL R+K DF
Sbjct: 66 CVVAVDSAGRQIPMAFLERVKEDF 89

[Close]

BLAST of CsGy5G027910 vs. TrEMBL
Match: tr|A0A0A0KX86|A0A0A0KX86_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G633190 PE=4 SV=1)

HSP 1 Score: 435.3 bits (1118), Expect = 8.3e-119
Identity = 218/222 (98.20%), Postives = 218/222 (98.20%), Query Frame = 0


Query: 1   MVSNPDLVLYSCIARGTIILAEFTREPNLDDLPFRCIEKTPPHHSIFSHTIRNRAYTFLI 60
           MVSNPDLVLYSCIARGTIILAEFTREPNLDDLPFRCIEKTPPHHSIFSHTIRNRAYTFLI
Sbjct: 1   MVSNPDLVLYSCIARGTIILAEFTREPNLDDLPFRCIEKTPPHHSIFSHTIRNRAYTFLI 60

Query: 61  EGSFVYFGIFHEDLNKSEVSGFLNRLKRDFEVFLESESLKGFENFTSHCFQSHFDSSFRH 120
           EGSFVYFGIFHEDLNKSEVSGFLNRLKRDFEVFLESESLKGFENFTSHCFQSHFDSSFRH
Sbjct: 61  EGSFVYFGIFHEDLNKSEVSGFLNRLKRDFEVFLESESLKGFENFTSHCFQSHFDSSFRH 120

Query: 121 LLGLNLNKTSSNGLKGSQRSSLDSSLRKQIPLVSKFSNPGSLMKKKKKVTGDGNWDGGKY 180
           LLGLNLNKTSSNGLKGSQRSSLDSSLRKQIPLVSKFSNPGSLMKKKKKVTGDGNWDGGKY
Sbjct: 121 LLGLNLNKTSSNGLKGSQRSSLDSSLRKQIPLVSKFSNPGSLMKKKKKVTGDGNWDGGKY 180

Query: 181 GMDDSVDLYDDNNGLCSRDITLLMQKHG----DFSALKAENA 219
           GMDDSVDLYDDNNGLCSRDITLLMQKHG    DFSALKAENA
Sbjct: 181 GMDDSVDLYDDNNGLCSRDITLLMQKHGSHNRDFSALKAENA 222

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BLAST of CsGy5G027910 vs. TrEMBL
Match: tr|A0A1S3C809|A0A1S3C809_CUCME (phytolongin Phyl2.1-like OS=Cucumis melo OX=3656 GN=LOC103498036 PE=4 SV=1)

HSP 1 Score: 402.9 bits (1034), Expect = 4.6e-109
Identity = 199/212 (93.87%), Postives = 204/212 (96.23%), Query Frame = 0


Query: 1   MVSNPDLVLYSCIARGTIILAEFTREPNLDDLPFRCIEKTPPHHSIFSHTIRNRAYTFLI 60
           MVSNPDLV YSCIARGTIILAEFTREPNLDDLP RCIEK PPHHSIFSHTIRNRAYTFLI
Sbjct: 1   MVSNPDLVFYSCIARGTIILAEFTREPNLDDLPLRCIEKAPPHHSIFSHTIRNRAYTFLI 60

Query: 61  EGSFVYFGIFHEDLNKSEVSGFLNRLKRDFEVFLESESLKGFENFTSHCFQSHFDSSFRH 120
           EGSFVYFGIFHEDLNKSEVSGFLNRLKRDFEVFLESESLKGFENFTS+CFQS FDSSFRH
Sbjct: 61  EGSFVYFGIFHEDLNKSEVSGFLNRLKRDFEVFLESESLKGFENFTSYCFQSQFDSSFRH 120

Query: 121 LLGLNLNKTSSNGLKGSQRSSLDSSLRKQIPLVSKFSNPGSLMKKKKKVTGDGNWDGGKY 180
           LLGLNLNKTSSNGL GSQRSSLDSS RKQIPLVSKFSNPGSLMKKKKKVTGDGNW+GGKY
Sbjct: 121 LLGLNLNKTSSNGLMGSQRSSLDSSTRKQIPLVSKFSNPGSLMKKKKKVTGDGNWEGGKY 180

Query: 181 GMDDSVDLYDDNNGLCSRDITLLMQKHGDFSA 213
           GMDDSVDLYDDNNGLCSRDITLLMQK+G  ++
Sbjct: 181 GMDDSVDLYDDNNGLCSRDITLLMQKNGSHNS 212

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BLAST of CsGy5G027910 vs. TrEMBL
Match: tr|A0A2P5E6S9|A0A2P5E6S9_9ROSA (Longin domain containing protein OS=Trema orientalis OX=63057 GN=TorRG33x02_229990 PE=3 SV=1)

HSP 1 Score: 182.6 bits (462), Expect = 9.7e-43
Identity = 107/214 (50.00%), Postives = 138/214 (64.49%), Query Frame = 0


Query: 1   MVSNPDLVLYSCIARGTIILAEFTREPNLDDLPFRCIEKTPPHHSIFSHTIRNRAYTFLI 60
           MVSNPDL+ Y CIAR T ILAEFT+EP L+DL  +CI++TPP+HS+FSHT+R R YTFLI
Sbjct: 1   MVSNPDLIYYVCIARDTTILAEFTKEPQLEDLARQCIQRTPPYHSMFSHTVRKRTYTFLI 60

Query: 61  EGSFVYFGIFHEDLNKSEVSGFLNRLKRDFEVFLESESLKGFENFTSHCFQSHFDSSFRH 120
           +  FVYF IF E L++SE   FLNR+K  FE      S +G EN +SHCFQ+ FDS  R 
Sbjct: 61  DDPFVYFAIFDEQLDQSEGLWFLNRVKTGFEE--GGGSKRGSENLSSHCFQAEFDSILRE 120

Query: 121 LLGLNLNKTSSNGLKG--SQRSSLDSSLRKQ----IPLVSKFSNPGSLMKKKKKVTGDGN 180
            +GL +    S       SQ  SLDSS   Q     PL+     P   +KKKK+++G+ N
Sbjct: 121 NIGLGVGLVDSTSSLSSVSQNPSLDSSRGGQSFGFTPLLG--VKPVKGLKKKKRLSGETN 180

Query: 181 WDGGKYG--MDDSVDLYDDNNGLCSRDITLLMQK 207
            D GK     +++VD+YDD NG+ +R+ T  MQK
Sbjct: 181 GDSGKDSTTAENTVDVYDDVNGVSNREFTTPMQK 210

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BLAST of CsGy5G027910 vs. TrEMBL
Match: tr|A0A2N9IVU8|A0A2N9IVU8_FAGSY (Uncharacterized protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS56141 PE=4 SV=1)

HSP 1 Score: 177.6 bits (449), Expect = 3.1e-41
Identity = 96/211 (45.50%), Postives = 137/211 (64.93%), Query Frame = 0


Query: 1   MVSNPDLVLYSCIARGTIILAEFTREPNLDDLPFRCIEKTPPHHSIFSHTIRNRAYTFLI 60
           M+SNPDL+LY CIA+GT IL+ +T+E  L+ L  +C+EKTPP+HS+FSHTIR R YTFLI
Sbjct: 1   MISNPDLILYVCIAKGTTILSHYTKELGLESLVLQCLEKTPPYHSMFSHTIRRRTYTFLI 60

Query: 61  EGSFVYFGIFHEDLNKSEVSGFLNRLKRDFEVFLESESLKGFENFTSHCFQSHFDSSFRH 120
           +  FVYF IF+ED+ +S+   FLNR+K  FE  +E+ +LK F+ F+SHC Q   D  FR 
Sbjct: 61  QDPFVYFAIFNEDMEQSQGVWFLNRVKCAFEEVMEAGALKDFDKFSSHCLQKVCDPIFRE 120

Query: 121 LLGLNL--NKTSSNGLKGSQRSSLDSSLRKQI---PLVSKFSNPGSLMKKKKKVTGDGNW 180
            + L+L    +S +  K S   SLD+ +  ++   PL+    N G   KKK+   G+ N 
Sbjct: 121 AMALDLRMGNSSWSDSKDSWNLSLDAGIGMKVAMAPLLGSKPNKGLKKKKKRASDGEVNG 180

Query: 181 DGGKYGMDDSVDLYDDNNGLCSRDITLLMQK 207
           D     ++  VD++DD NG+C +D +L +QK
Sbjct: 181 DSKDRSLEKKVDVFDDVNGVC-KDFSLPVQK 210

[Close]

BLAST of CsGy5G027910 vs. TrEMBL
Match: tr|A0A2P5DFJ7|A0A2P5DFJ7_PARAD (Longin domain containing protein OS=Parasponia andersonii OX=3476 GN=PanWU01x14_068930 PE=3 SV=1)

HSP 1 Score: 174.1 bits (440), Expect = 3.5e-40
Identity = 103/214 (48.13%), Postives = 133/214 (62.15%), Query Frame = 0


Query: 1   MVSNPDLVLYSCIARGTIILAEFTREPNLDDLPFRCIEKTPPHHSIFSHTIRNRAYTFLI 60
           MVSNPDL+ Y+CIAR T ILAEFT+EP L+DL  +CI++TPP+HS+FSHT+R R YTFLI
Sbjct: 1   MVSNPDLIYYACIARDTTILAEFTKEPQLEDLARQCIQRTPPYHSMFSHTVRKRTYTFLI 60

Query: 61  EGSFVYFGIFHEDLNKSEVSGFLNRLKRDFEVFLESESLKGFENFTSHCFQSHFDSSFRH 120
           +  FVYF IF E+L++SE   FLNR+K  FE      S +G EN  SHCFQ+ FDS  + 
Sbjct: 61  DDPFVYFAIFDEELDQSEGLWFLNRVKAGFEE--GGGSKRGSENLNSHCFQAEFDSILKE 120

Query: 121 LLGLNLNKTSSNGLKG--SQRSSLDSSLRKQ----IPLVSKFSNPGSLMKKKKKVTGDGN 180
            +GL++    S       SQ  SLDSS   Q     PL+     P         ++G+ N
Sbjct: 121 SVGLDMGLVDSTSSLSSVSQNLSLDSSRGGQSFGFTPLLG--VKPVXXXXXXXXLSGESN 180

Query: 181 WDGGKYG--MDDSVDLYDDNNGLCSRDITLLMQK 207
            D GK     ++SVD YDD NG+ +R+ T  MQK
Sbjct: 181 GDSGKDSTTAENSVDAYDDVNGVSNREFTSTMQK 210

The following BLAST results are available for this feature:

BLAST of CsGy5G027910 vs. NCBI nr


Match Name	E-value	Identity	Description
KGN52421.1	1.3e-118	98.20	hypothetical protein Csa_5G633190 [Cucumis sativus]	[more]
XP_004148677.1	8.4e-115	98.11	PREDICTED: probable VAMP-like protein At1g33475 [Cucumis sativus]	[more]
XP_008458710.1	6.9e-109	93.87	PREDICTED: phytolongin Phyl2.1-like [Cucumis melo]	[more]
XP_022938537.1	1.1e-79	75.60	phytolongin Phyl2.2-like [Cucurbita moschata]	[more]
XP_022133479.1	2.4e-77	71.63	phytolongin Phyl2.2-like [Momordica charantia]	[more]

BLAST of CsGy5G027910 vs. TAIR10


Match Name	E-value	Identity	Description
AT5G52990.1	1.2e-27	35.16	SNARE-like superfamily protein	[more]
AT4G27840.1	4.7e-27	39.90	SNARE-like superfamily protein	[more]
AT4G10170.1	3.4e-09	35.42	SNARE-like superfamily protein	[more]
AT2G32670.1	1.3e-08	30.95	vesicle-associated membrane protein 725	[more]
AT1G04760.1	2.2e-08	30.95	vesicle-associated membrane protein 726	[more]

BLAST of CsGy5G027910 vs. Swiss-Prot


Match Name	E-value	Identity	Description
sp\|Q9LVV3\|PHL22_ARATH	2.2e-26	35.16	Phytolongin Phyl2.2 OS=Arabidopsis thaliana OX=3702 GN=PHYL2.2 PE=2 SV=1	[more]
sp\|Q9STP2\|PHL21_ARATH	8.5e-26	39.90	Phytolongin Phyl2.1 OS=Arabidopsis thaliana OX=3702 GN=PHYL2.1 PE=2 SV=1	[more]
sp\|Q9SN26\|PHL11_ARATH	6.1e-08	35.42	Phytolongin Phyl1.1 OS=Arabidopsis thaliana OX=3702 GN=PHYL1.1 PE=2 SV=1	[more]
sp\|O48850\|VA725_ARATH	2.3e-07	30.95	Vesicle-associated membrane protein 725 OS=Arabidopsis thaliana OX=3702 GN=VAMP7...	[more]
sp\|P47192\|VA722_ARATH	3.0e-07	30.95	Vesicle-associated membrane protein 722 OS=Arabidopsis thaliana OX=3702 GN=VAMP7...	[more]

BLAST of CsGy5G027910 vs. TrEMBL


Match Name	E-value	Identity	Description
tr\|A0A0A0KX86\|A0A0A0KX86_CUCSA	8.3e-119	98.20	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G633190 PE=4 SV=1	[more]
tr\|A0A1S3C809\|A0A1S3C809_CUCME	4.6e-109	93.87	phytolongin Phyl2.1-like OS=Cucumis melo OX=3656 GN=LOC103498036 PE=4 SV=1	[more]
tr\|A0A2P5E6S9\|A0A2P5E6S9_9ROSA	9.7e-43	50.00	Longin domain containing protein OS=Trema orientalis OX=63057 GN=TorRG33x02_2299...	[more]
tr\|A0A2N9IVU8\|A0A2N9IVU8_FAGSY	3.1e-41	45.50	Uncharacterized protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS56141 PE=4 SV=1	[more]
tr\|A0A2P5DFJ7\|A0A2P5DFJ7_PARAD	3.5e-40	48.13	Longin domain containing protein OS=Parasponia andersonii OX=3476 GN=PanWU01x14_...	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR011012	Longin-like_dom_sf

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0008150	biological_process
cellular_component	GO:0016021	integral component of membrane
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0003674	molecular_function

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CsGy5G027910.1	CsGy5G027910.1	mRNA

InterPro

Analysis Name: InterPro Annotations of cucumber Gy14 genome (v2)
Date Performed: 2018-09-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	G3DSA:3.30.450.50		coord: 2..132 e-value: 1.6E-15 score: 59.1
None	No IPR available	PANTHER	PTHR21136	SNARE PROTEINS	coord: 1..208
None	No IPR available	PANTHER	PTHR21136:SF93	PHYTOLONGIN PHYL2.1-RELATED	coord: 1..208
IPR011012	Longin-like domain superfamily	SUPERFAMILY	SSF64356	SNARE-like	coord: 8..95

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
CsGy5G027910	Cla010362	Watermelon (97103) v1	cgybwmB340
CsGy5G027910	Csa5G633190	Cucumber (Chinese Long) v2	cgybcuB223
CsGy5G027910	MELO3C021488	Melon (DHL92) v3.5.1	cgybmeB299
CsGy5G027910	ClCG09G018560	Watermelon (Charleston Gray)	cgybwcgB315
CsGy5G027910	CSPI05G28590	Wild cucumber (PI 183967)	cgybcpiB225
CsGy5G027910	CmaCh15G003000	Cucurbita maxima (Rimu)	cgybcmaB609
CsGy5G027910	CmaCh04G027380	Cucurbita maxima (Rimu)	cgybcmaB647
CsGy5G027910	Lsi02G015190	Bottle gourd (USVL1VR-Ls)	cgyblsiB319
CsGy5G027910	Cp4.1LG01g23010	Cucurbita pepo (Zucchini)	cgybcpeB625
CsGy5G027910	Cp4.1LG13g08600	Cucurbita pepo (Zucchini)	cgybcpeB598
CsGy5G027910	MELO3C021488.2	Melon (DHL92) v3.6.1	cgybmedB299
CsGy5G027910	CsaV3_5G037670	Cucumber (Chinese Long) v3	cgybcucB232
CsGy5G027910	Cla97C09G179980	Watermelon (97103) v2	cgybwmbB390
CsGy5G027910	Bhi06G000386	Wax gourd	cgybwgoB447
CsGy5G027910	Carg04411	Silver-seed gourd	carcgybB0686
CsGy5G027910	Carg02273	Silver-seed gourd	carcgybB0280

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
CsGy5G027910	Cucumber (Gy14) v2	cgybcgybB032
CsGy5G027910	Cucurbita moschata (Rifu)	cgybcmoB584
CsGy5G027910	Cucurbita moschata (Rifu)	cgybcmoB625
CsGy5G027910	Cucumber (Chinese Long) v2	cgybcuB203
CsGy5G027910	Bottle gourd (USVL1VR-Ls)	cgyblsiB324
CsGy5G027910	Melon (DHL92) v3.5.1	cgybmeB331
CsGy5G027910	Melon (DHL92) v3.6.1	cgybmedB333
CsGy5G027910	Watermelon (Charleston Gray)	cgybwcgB319
CsGy5G027910	Watermelon (Charleston Gray)	cgybwcgB338
CsGy5G027910	Wild cucumber (PI 183967)	cgybcpiB206
CsGy5G027910	Cucumber (Chinese Long) v3	cgybcucB207
CsGy5G027910	Watermelon (97103) v2	cgybwmbB326
CsGy5G027910	Wax gourd	cgybwgoB475