Cp4.1LG20g05580 (gene) Cucurbita pepo (Zucchini)

NameCp4.1LG20g05580
Typegene
OrganismCucurbita pepo (Cucurbita pepo (Zucchini))
DescriptionPectinesterase
LocationCp4.1LG20 : 3376560 .. 3379100 (+)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTCTCTCCTCAGACCTTATGGTAAGGTCGATGAATCCGATCAAATGAGGCTCGACGCTAGCCGACGGACTCGGAAGAGGGTCATTGTCATTGTCTTGTCCTCTGTTGTTCTTGTTGGTGTTGTTGTTGCTGTCGTGTTCGGGACTAGAAATAGTTCCAAGAACGACGAGAGTGACAATCACAATGAGGCTCTTTCAACTTCGATAAAAGCAGTTTGTGATGAGACTTTGTATCCCGATACATGTAAGAATGCTTTTGGTTCTCTAGCTAACTCGAGCCATCTCGACCCTGGTCAAATAGCCAAGTTTTCGGTTCAACTCGCGCTCGGTGAACTGTCGCGTGTGGCTGATTACCTTTTGGAGCATGCCATTACCAATTCAACTGATAATAAGACCATATTAGCTTTGAGAAGTTGTCATGAACTTTTGGAGTTGGCAATAGATCATCTAAATGACTCATTATCATCTAGTGAGATTACAGTGCTGAAAGCTGTGGATGATTTGAAAACATGGCTAAGTTCAGCAGCTACTTACCAGCAAACTTGCATCGACGGGCTCGAGGAAGTCGATCTCGCTTTGACGAATGTCATTGCAAATTACTTGAAGAACTCTACTGAAATGACCAGCAATGGCCTTGCCATTGTGTCCTTCTTTTCGAAGCTAACGGATTCTTTCAGTCTACGACGATTGATGAGTTATGACGATCGACATAGCAATGGAGATTGGGCGCGCCCGATACTCCGGAAGCTAGCTCAAAAAGATCTCCGGAAGCAGGCCGACATCGTCGTAGCGAAAGACAAATCGGGGAAGTACAAAACAATCACTGAAGCTCTCAACGCTGTTCCTGATAAGAGCAAGAAGAGAACTGTGATCTATGTGAAGAAGGGAGTTTACAAGGAGAATGTTGAAGTTGTGAAGAACAAATGGAATGTAGTGATGATTGGCGATGGAATGACTTTAACCACTGTAACTGGCAGGCTCAACTTCATAGATGGGACGCCAACATTTTCTACTGCAACATTTGGTAACATTATCTAAGCTTACGAACGTCCCACATTGGCTAATTTAGGGAATGATCATGAGTTTATAAGTAAGGAATACATCTTCATTAGTACGAGGTCTTTTGGGGAAGCCCAAAGTAAAGCCACGAGAGCTTATGCTTAAAGTGGACAATATCGTACCATTGTGGAGGGTCGTGATTCCTAACATGGTATCAGAGCTATGCCCTGAACTTAGCTATGTCAATAGAATCCTCAAATGTCGAACAAAGAATCGTGAGGCTCGAAGGTGTAGTTAAAAGTGACTAATGTGTCGAACAAAGGGTGTACTTTGTTCGAGGACTCCAGAAAAGGAGTCGAGCCTTGATTAAGGGGAGATTGTTCGATGGGTACATAGACTTTAGGGGAGGCTCTATAGTATACTTTGTTCAAGGGGAGCATTGGTGTGAGGGAAGCCTAAAGTAAAGCTAGAGCTTAGGCTCAAAGTGGACAATATCATACTAGTGTGGAGAGTCGTGATTCCTAGCAAGCTTAGAATCTAACAATGTTTTGTGTTTTTTTTGTGCAGCTGCAAAGGGAAAAGGCTTCATTGCCATAGACATGGGATTTGAGAACACGGCTGGACCGAGCAAACACCAAGCGGTTGCTCTGATGTCGACGTCGGACCAATCGATCTTCTACCGCTGCCAAATGAATGCATACCAAGACACTCTCTATGCACACTCGAACCGTCAATTCTACCGTGACTGCAAAGTTTACGGCACGGTCGATTTCATCTTTGGAAACTCAGCAGTAGTCCTACAAAACTGCAAAATTGCACCAAAGTTGCCTCTTTTAGGCCAAAAGAACACAATCACTGCTCAAGGCCGGTTTGATCCTAATCAACACACTGGCATTTCAATACAACACTCTTCAATCCAACCGTTCGAAAACTTGAAAACTACCGAAACTTACCTCGGTCGTCCATGGAAGAACTACTCGACGACCGTGTTTATGCAGAACGATTTCGGGAGGTTGATTCACCCAACTGGCTGGCTGCCATGGGTTGGAACATCAGCACCAGACACCATTTTCTATGCTGAGTTTCAAAACAAAGGCCCTGGTTCTTCCACAGCCAACAGAGTGAAATGGAAAGGTTTGAAGAATATTGACACCAAAACTGCCAAGAAATTCACTGTTTCCTCTTTCATTCATGGTAAAGATTGGATCTCAAAGGCTCAAGTCCCCTTCAATGCCACCCTCTGAGTTTCATTTTCATTTCATGGACTTCCACTCAATGCTTTTGTCTTCAAATTCTGTTGTTTCATATTGTTTACTTCTTTTTAACCATTTGATTGATTGGTATTGTGATAAGAACATATACTGAGAAAGTTCTGTCCACTTTTTGGGCTTGAATTCGACACTTTTAATGTTTATGAGGTCTTTTAAGTTGCATTCAAACTGTAATTCTCTTATCAGCTCCCTTCAAAATATCAAAACCATGAAAACAATTGTCTTGATGTAGTGGCTTTCTTTCCGACTTCATCCGCGACACCCGGGTTGA

mRNA sequence

ATGTCTCTCCTCAGACCTTATGGTAAGGTCGATGAATCCGATCAAATGAGGCTCGACGCTAGCCGACGGACTCGGAAGAGGGTCATTGTCATTGTCTTGTCCTCTGTTGTTCTTGTTGGTGTTGTTGTTGCTGTCGTGTTCGGGACTAGAAATAGTTCCAAGAACGACGAGAGTGACAATCACAATGAGGCTCTTTCAACTTCGATAAAAGCAGTTTGTGATGAGACTTTGTATCCCGATACATGTAAGAATGCTTTTGGTTCTCTAGCTAACTCGAGCCATCTCGACCCTGGTCAAATAGCCAAGTTTTCGGTTCAACTCGCGCTCGGTGAACTGTCGCGTGTGGCTGATTACCTTTTGGAGCATGCCATTACCAATTCAACTGATAATAAGACCATATTAGCTTTGAGAAGTTGTCATGAACTTTTGGAGTTGGCAATAGATCATCTAAATGACTCATTATCATCTAGTGAGATTACAGTGCTGAAAGCTGTGGATGATTTGAAAACATGGCTAAGTTCAGCAGCTACTTACCAGCAAACTTGCATCGACGGGCTCGAGGAAGTCGATCTCGCTTTGACGAATGTCATTGCAAATTACTTGAAGAACTCTACTGAAATGACCAGCAATGGCCTTGCCATTGTGTCCTTCTTTTCGAAGCTAACGGATTCTTTCAGTCTACGACGATTGATGAGTTATGACGATCGACATAGCAATGGAGATTGGGCGCGCCCGATACTCCGGAAGCTAGCTCAAAAAGATCTCCGGAAGCAGGCCGACATCGTCGTAGCGAAAGACAAATCGGGGAAGTACAAAACAATCACTGAAGCTCTCAACGCTGTTCCTGATAAGAGCAAGAAGAGAACTGTGATCTATGTGAAGAAGGGAGTTTACAAGGAGAATGTTGAAGTTGTGAAGAACAAATGGAATGTAGTGATGATTGGCGATGGAATGACTTTAACCACTTGGCTTTCTTTCCGACTTCATCCGCGACACCCGGGTTGA

Coding sequence (CDS)

ATGTCTCTCCTCAGACCTTATGGTAAGGTCGATGAATCCGATCAAATGAGGCTCGACGCTAGCCGACGGACTCGGAAGAGGGTCATTGTCATTGTCTTGTCCTCTGTTGTTCTTGTTGGTGTTGTTGTTGCTGTCGTGTTCGGGACTAGAAATAGTTCCAAGAACGACGAGAGTGACAATCACAATGAGGCTCTTTCAACTTCGATAAAAGCAGTTTGTGATGAGACTTTGTATCCCGATACATGTAAGAATGCTTTTGGTTCTCTAGCTAACTCGAGCCATCTCGACCCTGGTCAAATAGCCAAGTTTTCGGTTCAACTCGCGCTCGGTGAACTGTCGCGTGTGGCTGATTACCTTTTGGAGCATGCCATTACCAATTCAACTGATAATAAGACCATATTAGCTTTGAGAAGTTGTCATGAACTTTTGGAGTTGGCAATAGATCATCTAAATGACTCATTATCATCTAGTGAGATTACAGTGCTGAAAGCTGTGGATGATTTGAAAACATGGCTAAGTTCAGCAGCTACTTACCAGCAAACTTGCATCGACGGGCTCGAGGAAGTCGATCTCGCTTTGACGAATGTCATTGCAAATTACTTGAAGAACTCTACTGAAATGACCAGCAATGGCCTTGCCATTGTGTCCTTCTTTTCGAAGCTAACGGATTCTTTCAGTCTACGACGATTGATGAGTTATGACGATCGACATAGCAATGGAGATTGGGCGCGCCCGATACTCCGGAAGCTAGCTCAAAAAGATCTCCGGAAGCAGGCCGACATCGTCGTAGCGAAAGACAAATCGGGGAAGTACAAAACAATCACTGAAGCTCTCAACGCTGTTCCTGATAAGAGCAAGAAGAGAACTGTGATCTATGTGAAGAAGGGAGTTTACAAGGAGAATGTTGAAGTTGTGAAGAACAAATGGAATGTAGTGATGATTGGCGATGGAATGACTTTAACCACTTGGCTTTCTTTCCGACTTCATCCGCGACACCCGGGTTGA

Protein sequence

MSLLRPYGKVDESDQMRLDASRRTRKRVIVIVLSSVVLVGVVVAVVFGTRNSSKNDESDNHNEALSTSIKAVCDETLYPDTCKNAFGSLANSSHLDPGQIAKFSVQLALGELSRVADYLLEHAITNSTDNKTILALRSCHELLELAIDHLNDSLSSSEITVLKAVDDLKTWLSSAATYQQTCIDGLEEVDLALTNVIANYLKNSTEMTSNGLAIVSFFSKLTDSFSLRRLMSYDDRHSNGDWARPILRKLAQKDLRKQADIVVAKDKSGKYKTITEALNAVPDKSKKRTVIYVKKGVYKENVEVVKNKWNVVMIGDGMTLTTWLSFRLHPRHPG
BLAST of Cp4.1LG20g05580 vs. Swiss-Prot
Match: PME24_ARATH (Putative pectinesterase/pectinesterase inhibitor 24 OS=Arabidopsis thaliana GN=PME24 PE=3 SV=1)

HSP 1 Score: 263.8 bits (673), Expect = 2.5e-69
Identity = 150/320 (46.88%), Postives = 220/320 (68.75%), Query Frame = 1

Query: 6   PYGKVDESDQMRLDASRRTRKRVIVIVLSSVVLVGVVVAVVFGT---RNSSKNDESDNHN 65
           PYGKVDE + +RL+A R+TRK + +I +S V+L G+V+  VFGT   + S +  E++N+ 
Sbjct: 4   PYGKVDEREHVRLEARRKTRKNIAIIAVSLVILAGIVIGAVFGTMAHKKSPETVETNNNG 63

Query: 66  EALSTSIKAVCDETLYPDTCKNAFGSLANSSHLDPGQIAKFSVQLALGELSRVADYLLEH 125
           +++S S+KAVCD TL+ + C    GS  N+S L+P ++ +++V++ + E+S+  +     
Sbjct: 64  DSISVSVKAVCDVTLHKEKCFETLGSAPNASSLNPEELFRYAVKITIAEVSKAIN----- 123

Query: 126 AITNST-DNKTILALRSCHELLELAIDHLNDSLSSS---EITVLKAVDDLKTWLSSAATY 185
           A ++S  D K  + + +C ELL+L ID+LN++L+SS   ++TV + VDDL+TWLSSA TY
Sbjct: 124 AFSSSLGDEKNNITMNACAELLDLTIDNLNNTLTSSSNGDVTVPELVDDLRTWLSSAGTY 183

Query: 186 QQTCIDGLEEVDLALTNVIANYLKNSTEMTSNGLAIVSFFSKLTDSFSLRR-LMSYDDRH 245
           Q+TC++ L      +     ++LKNSTE+TSN LAI+++  K+ DSF LRR L++  D  
Sbjct: 184 QRTCVETLAP---DMRPFGESHLKNSTELTSNALAIITWLGKIADSFKLRRRLLTTADVE 243

Query: 246 SNGDWARPILRKLAQKDLRKQADIVVAKDKSGKYKTITEALNAVPDKSKKRTVIYVKKGV 305
            +    R   R L   DLRK ADIVVAKD SGKY+TI  AL  VP+KS+KRT+IYVKKGV
Sbjct: 244 VDFHAGR---RLLQSTDLRKVADIVVAKDGSGKYRTIKRALQDVPEKSEKRTIIYVKKGV 303

Query: 306 YKENVEVVKNKWNVVMIGDG 318
           Y ENV+V K  WNV+++GDG
Sbjct: 304 YFENVKVEKKMWNVIVVGDG 312

BLAST of Cp4.1LG20g05580 vs. Swiss-Prot
Match: PME46_ARATH (Probable pectinesterase/pectinesterase inhibitor 46 OS=Arabidopsis thaliana GN=PME46 PE=2 SV=1)

HSP 1 Score: 260.0 bits (663), Expect = 3.6e-68
Identity = 153/324 (47.22%), Postives = 217/324 (66.98%), Query Frame = 1

Query: 7   YGKVDESDQMRLDASRRTRKRVIVIVLSSVVLVGVVVAVVFGT--RNSSKNDESDNHNEA 66
           YG++DE +Q +L+ASR+T+KR+ +I +SS+VLV +VV  V GT  R++SK   ++N+ E 
Sbjct: 4   YGRLDEHEQAKLEASRKTKKRIAIIAISSIVLVCIVVGAVVGTTARDNSKKPPTENNGEP 63

Query: 67  LSTSIKAVCDETLYPDTCKNAFGSLANSSHLDPGQIAKFSVQLALGELSRVADYLL--EH 126
           +S S+KA+CD TL+ + C    GS  N+S   P ++ K++V++ + ELS+V D     EH
Sbjct: 64  ISVSVKALCDVTLHKEKCFETLGSAPNASRSSPEELFKYAVKVTITELSKVLDGFSNGEH 123

Query: 127 AITNSTDNKTILALRSCHELLELAIDHLNDSLSSSEITVLKAVDDLKTWLSSAATYQQTC 186
                 DN T  A+ +C EL+ LA+D LN++++SS    LK  DDL+TWLSS  TYQ+TC
Sbjct: 124 M-----DNATSAAMGACVELIGLAVDQLNETMTSS----LKNFDDLRTWLSSVGTYQETC 183

Query: 187 IDGLEEVDL-ALTNVIANYLKNSTEMTSNGLAIVSFFSKLTDSFSLRRLMSYDDRHSNGD 246
           +D L E +  +LT    N+LKNSTEMTSN LAI+++  K+ D+   RR    +  ++   
Sbjct: 184 MDALVEANKPSLTTFGENHLKNSTEMTSNALAIITWLGKIADTVKFRRRRLLETGNAKVV 243

Query: 247 WAR-PIL---RKLAQKDLRKQADIVVAKDKSGKYKTITEALNAVPDKSKKRTVIYVKKGV 306
            A  P++   R L   DL+K+A IVVAKD SGKY+TI EAL  V +K++K T+IYVKKGV
Sbjct: 244 VADLPMMEGRRLLESGDLKKKATIVVAKDGSGKYRTIGEALAEVEEKNEKPTIIYVKKGV 303

Query: 307 YKENVEVVKNKWNVVMIGDGMTLT 322
           Y ENV V K KWNVVM+GDG + T
Sbjct: 304 YLENVRVEKTKWNVVMVGDGQSKT 318

BLAST of Cp4.1LG20g05580 vs. Swiss-Prot
Match: PME3_PHAVU (Pectinesterase 3 OS=Phaseolus vulgaris GN=MPE3 PE=2 SV=1)

HSP 1 Score: 178.7 bits (452), Expect = 1.0e-43
Identity = 123/336 (36.61%), Postives = 188/336 (55.95%), Query Frame = 1

Query: 7   YGKVDESDQMRLDASRRTRKRVIVIVLSSVVLVGVVVAVVFGTRNSSKNDESDNHNEAL- 66
           YGKV+E +Q   +  ++TRKR+I+I +SS+VL+ V++A V G    ++N ES   ++++ 
Sbjct: 10  YGKVNELEQQAYE--KKTRKRLIIIAVSSIVLIAVIIAAVAGVVIHNRNSESSPSSDSVP 69

Query: 67  ------STSIKAVCDETLYPDTCKNAFGSLANSSHLDPGQIAKFSVQLALGELSRVADYL 126
                 + S+KAVCD T YP +C ++  SL  S+  DP  + K S+++A+ ELS     L
Sbjct: 70  QTELSPAASLKAVCDTTRYPSSCFSSISSLPESNTTDPELLFKLSLRVAIDELSSFPSKL 129

Query: 127 LEHAITNSTDNKTILALRSCHELLELAIDHLNDSLSS-----SEITVLKAVDDLKTWLSS 186
             +A     D +   A+  C  +   A+D LNDS+S+       I    +V +++TWLS+
Sbjct: 130 RANA---EQDARLQKAIDVCSSVFGDALDRLNDSISALGTVAGRIASSASVSNVETWLSA 189

Query: 187 AATYQQTCIDGLEEVDLALTN----VIANYLKNSTEMTSNGLAIVSFFSKLTDSFSL--- 246
           A T Q TC+D + E++          I   ++NSTE  SN LAIV+    L   F     
Sbjct: 190 ALTDQDTCLDAVGELNSTAARGALQEIETAMRNSTEFASNSLAIVTKILGLLSRFETPIH 249

Query: 247 -RRLMSYDDRHSNGDWARPILRKLAQ-KDLRKQADIVVAKDKSGKYKTITEALNAVPDKS 306
            RRL+ + +      W     R+L + K+     D VVAKD SG++KTI EAL  V  KS
Sbjct: 250 HRRLLGFPE------WLGAAERRLLEEKNNDSTPDAVVAKDGSGQFKTIGEALKLVKKKS 309

Query: 307 KKRTVIYVKKGVYKENVEVVKNKWNVVMIGDGMTLT 322
           ++R  +YVK+G Y EN+++ KN WNV++ GDG   T
Sbjct: 310 EERFSVYVKEGRYVENIDLDKNTWNVMIYGDGKDKT 334

BLAST of Cp4.1LG20g05580 vs. Swiss-Prot
Match: PME1_ARATH (Pectinesterase 1 OS=Arabidopsis thaliana GN=PME1 PE=1 SV=1)

HSP 1 Score: 178.7 bits (452), Expect = 1.0e-43
Identity = 127/335 (37.91%), Postives = 191/335 (57.01%), Query Frame = 1

Query: 7   YGKVDESDQMRLDASRRTRKRVIVIVLSSVVLVGVVVAVVFGT---RNSSKNDESDNHNE 66
           YGKVDE+  + L   ++TRKR++++ +S VVL+ V++A V  T   +N +++  S     
Sbjct: 10  YGKVDEAQDLAL--KKKTRKRLLLLSISVVVLIAVIIAAVVATVVHKNKNESTPSPPPEL 69

Query: 67  ALSTSIKAVCDETLYPDTCKNAFGSLANSSHLDPGQIAKFSVQLALGELSRVADYLLEHA 126
             STS+KA+C  T +P++C ++   L +S+  DP  + K S+++ + EL  ++D L E  
Sbjct: 70  TPSTSLKAICSVTRFPESCISSISKLPSSNTTDPETLFKLSLKVIIDELDSISD-LPEKL 129

Query: 127 ITNSTDNKTILALRSCHELLELAIDHLNDSLSSSEITVLKA------VDDLKTWLSSAAT 186
              + D +   ALR C +L+E A+D LND++S+ +    K       ++DLKTWLS+  T
Sbjct: 130 SKETEDERIKSALRVCGDLIEDALDRLNDTVSAIDDEEKKKTLSSSKIEDLKTWLSATVT 189

Query: 187 YQQTCIDGLEEV--------DLALTNVIANYLKNSTEMTSNGLAIVS-FFSKLTDS---- 246
             +TC D L+E+        +  +T  + + +  STE TSN LAIVS   S L+D     
Sbjct: 190 DHETCFDSLDELKQNKTEYANSTITQNLKSAMSRSTEFTSNSLAIVSKILSALSDLGIPI 249

Query: 247 FSLRRLMSYDDRHSNG--DWARPILRKLAQKDLRKQADIVVAKDKSGKYKTITEALNAVP 306
              RRLMS+  + S     WAR   R+L Q    K  D+ VA D +G   T+ EA+  VP
Sbjct: 250 HRRRRLMSHHHQQSVDFEKWAR---RRLLQTAGLKP-DVTVAGDGTGDVLTVNEAVAKVP 309

Query: 307 DKSKKRTVIYVKKGVYKENVEVVKNKWNVVMIGDG 318
            KS K  VIYVK G Y ENV + K+KWNV++ GDG
Sbjct: 310 KKSLKMFVIYVKSGTYVENVVMDKSKWNVMIYGDG 337

BLAST of Cp4.1LG20g05580 vs. Swiss-Prot
Match: PME58_ARATH (Probable pectinesterase/pectinesterase inhibitor 58 OS=Arabidopsis thaliana GN=PME58 PE=2 SV=1)

HSP 1 Score: 145.2 bits (365), Expect = 1.3e-33
Identity = 106/323 (32.82%), Postives = 170/323 (52.63%), Query Frame = 1

Query: 16  MRLDASRRTRKRVIVIVLSSVVLVGVVVAVVFGTRNSSKNDES-DNHNEALSTSIKAVCD 75
           M +D   + +K +I  V+++++++ VV   +  +RN+S +++      +  +T+++AVC 
Sbjct: 1   MGVDGELKKKKCIIAGVITALLVLMVVAVGITTSRNTSHSEKIVPVQIKTATTAVEAVCA 60

Query: 76  ETLYPDTCKNAFGSLANSSHLDPGQIAKFSVQLALGELS-RVADYLLEHAITNSTDNKTI 135
            T Y +TC N+    A+     P  + K    + +  +   +    +E     + D  T 
Sbjct: 61  PTDYKETCVNSLMK-ASPDSTQPLDLIKLGFNVTIRSIEDSIKKASVELTAKAANDKDTK 120

Query: 136 LALRSCHELLELAIDHLNDSLSS----SEITVLKAVDDLKTWLSSAATYQQTCIDGLEEV 195
            AL  C +L+  A D L   L +    S   +   V+DL+ WLS +  YQQTC+D  EE 
Sbjct: 121 GALELCEKLMNDATDDLKKCLDNFDGFSIPQIEDFVEDLRVWLSGSIAYQQTCMDTFEET 180

Query: 196 DLALTNVIANYLKNSTEMTSNGLAIVSFFSKLTDSFSL-----------RRLMSYDDRHS 255
           +  L+  +    K S E+TSNGLA+++  S L   F++           R+L+S +D   
Sbjct: 181 NSKLSQDMQKIFKTSRELTSNGLAMITNISNLLGEFNVTGVTGDLGKYARKLLSAEDGIP 240

Query: 256 NGDWARPILRKLAQKDLRKQADIVVAKDKSGKYKTITEALNAVPDKSKKRTVIYVKKGVY 315
           +  W  P  R+L       +A++VVA D SG+YKTI EALNAVP  ++K  VIY+K+GVY
Sbjct: 241 S--WVGPNTRRLMATKGGVKANVVVAHDGSGQYKTINEALNAVPKANQKPFVIYIKQGVY 300

Query: 316 KENVEVVKNKWNVVMIGDGMTLT 322
            E V+V K   +V  IGDG T T
Sbjct: 301 NEKVDVTKKMTHVTFIGDGPTKT 320

BLAST of Cp4.1LG20g05580 vs. TrEMBL
Match: A0A0A0LY08_CUCSA (Pectinesterase OS=Cucumis sativus GN=Csa_1G538170 PE=4 SV=1)

HSP 1 Score: 512.3 bits (1318), Expect = 4.4e-142
Identity = 261/322 (81.06%), Postives = 296/322 (91.93%), Query Frame = 1

Query: 1   MSLLRPYGKVDESDQMRLDASRRTRKRVIVIVLSSVVLVGVVVAVVFGTRNSSKN-DESD 60
           MS LRPYGKVDE+DQ+RLDA RRTR+R++VIVLSSVVLV VVVAVVFGTRN+S N D+S+
Sbjct: 1   MSFLRPYGKVDETDQLRLDARRRTRRRIVVIVLSSVVLVAVVVAVVFGTRNASNNNDDSN 60

Query: 61  NHNEALSTSIKAVCDETLYPDTCKNAFGSLANSSHLDPGQIAKFSVQLALGELSRVADYL 120
           N N +LSTSIKAVCD TLYPDTC+ AFG +ANSSHLDPGQI+K SVQLALGELS+VADY+
Sbjct: 61  NQNGSLSTSIKAVCDVTLYPDTCQKAFGPIANSSHLDPGQISKLSVQLALGELSKVADYI 120

Query: 121 LEHAITNSTDNKTILALRSCHELLELAIDHLNDSLSSSEITVLKAVDDLKTWLSSAATYQ 180
            +HAIT + DNKTILAL++CHELL+LA+DHLN SLSSS+IT+LKAVDDLKTW++SAATYQ
Sbjct: 121 FDHAITKTADNKTILALKNCHELLDLALDHLNISLSSSDITLLKAVDDLKTWITSAATYQ 180

Query: 181 QTCIDGLEEVDLALTNVIANYLKNSTEMTSNGLAIVSFFSKLTDSFSLRRLMSYDDRHSN 240
           QTCID L EVD AL +++AN+LKNSTE+TSNGLAIVSFFSKLTDS SLRRLMSY++  SN
Sbjct: 181 QTCIDDLAEVDPALADLVANFLKNSTELTSNGLAIVSFFSKLTDSLSLRRLMSYENHQSN 240

Query: 241 GDWARPILRKLAQKDLRKQADIVVAKDKSGKYKTITEALNAVPDKSKKRTVIYVKKGVYK 300
           GDWARPILRKLAQ D+RK ADIVVAKD+SGKYKTITEAL AVPDKSKKRTVIYVKKG+YK
Sbjct: 241 GDWARPILRKLAQSDIRKHADIVVAKDRSGKYKTITEALGAVPDKSKKRTVIYVKKGIYK 300

Query: 301 ENVEVVKNKWNVVMIGDGMTLT 322
           ENVEVVKNKWNVVM+GDGMT T
Sbjct: 301 ENVEVVKNKWNVVMVGDGMTAT 322

BLAST of Cp4.1LG20g05580 vs. TrEMBL
Match: A0A061FYJ8_THECC (Pectinesterase OS=Theobroma cacao GN=TCM_045262 PE=4 SV=1)

HSP 1 Score: 313.9 bits (803), Expect = 2.3e-82
Identity = 176/326 (53.99%), Postives = 229/326 (70.25%), Query Frame = 1

Query: 2   SLLRPYGKVDESDQMRLDASRRTRKRVIVIVLSSVVLVGVVVAVVFGTRNSSKNDESDNH 61
           ++ R YGKV+E+DQ RL A R+TR+R+ +I LS +VL  +VVA VFG+R S  + ++  +
Sbjct: 32  NMFRAYGKVNEADQARLLARRKTRRRIAIIGLSFIVLAAIVVAAVFGSRGSGGDSKNGGN 91

Query: 62  N--EALSTSIKAVCDETLYPDTCKNAFGSLANSSHLDPGQIAKFSVQLALGELSRVADYL 121
              + +S+SIKAVCD TLY DTC ++   +ANSS L P  I K S+++A+ ELS+ + Y 
Sbjct: 92  GGVQPISSSIKAVCDVTLYKDTCYDSLAPMANSSQLQPEDIFKLSMKVAIAELSKASQYF 151

Query: 122 LEHAITNST-DNKTILALRSCHELLELAIDHLNDSLSSS-EITVLKAVDDLKTWLSSAAT 181
            ++ I     D  +I AL +C ELL LAIDHLN SLSSS E++V++AVDDL+TWLSSA+T
Sbjct: 152 SKNGIFKDVADKMSIAALENCGELLSLAIDHLNSSLSSSGEVSVIQAVDDLRTWLSSAST 211

Query: 182 YQQTCIDGLEEVDLALTNVIANYLKNSTEMTSNGLAIVSFFSKLTDSFSLRRLMSYDDRH 241
           YQQTCID  EE+       + ++LK S+E+TSN LAI+++ SK+    +LRRLMS    H
Sbjct: 212 YQQTCIDAFEELKGDTKASVHDHLKYSSELTSNSLAIITWISKVASVLNLRRLMSSSPNH 271

Query: 242 SNGDWARPILRKLAQKD--LRKQADIVVAKDKSGKYKTITEALNAVPDKSKKRTVIYVKK 301
              +W     RKL Q    L+K+ADI+VAKD SGKYKTI+ AL AVPDKSKKR VIYVKK
Sbjct: 272 EEPEWFHVKDRKLLQSSDLLKKKADIIVAKDGSGKYKTISAALKAVPDKSKKRIVIYVKK 331

Query: 302 GVYKENVEVVKNKWNVVMIGDGMTLT 322
           GVY ENV V K KWNV MIGDGM  T
Sbjct: 332 GVYSENVSVEKKKWNVTMIGDGMEST 357

BLAST of Cp4.1LG20g05580 vs. TrEMBL
Match: M5XPH8_PRUPE (Pectinesterase OS=Prunus persica GN=PRUPE_ppa024156mg PE=4 SV=1)

HSP 1 Score: 312.4 bits (799), Expect = 6.7e-82
Identity = 179/331 (54.08%), Postives = 228/331 (68.88%), Query Frame = 1

Query: 1   MSLLRPYGKVDESDQMRLDASRRTRKRVIVIVLSSVVLVGVVVAVVFGTRNSSKNDESDN 60
           MS  + YGKV+E +Q RLDA R+TR+RV++I LSS+VLV VVVA V GT    KN+    
Sbjct: 1   MSSFKAYGKVNELEQSRLDARRKTRRRVVIISLSSIVLVCVVVAAVVGT-TIHKNNSGGK 60

Query: 61  HNEALSTSIKAVCDETLYPDTCKNAFGSLANSSHLDPGQIAKFSVQLALGELSRVADYLL 120
              +LSTS+KAVCD TLY D+C ++ G+L NS++  P  + K ++++A+ ELS+ A    
Sbjct: 61  PGSSLSTSVKAVCDVTLYQDSCYSSLGALVNSTNAKPEDLFKLAIRVAINELSKAASRFS 120

Query: 121 EHAITNSTDNKTI--LALRSCHELLELAIDHLNDSLSSSEITVLKAVDDLKTWLSSAATY 180
              I N T + T+   AL +C ELL LA+DHLNDSLS  + ++    DDLKTWLSS+ TY
Sbjct: 121 GDGILNGTSHDTLSKAALENCQELLSLALDHLNDSLSDDK-SLFDVFDDLKTWLSSSGTY 180

Query: 181 QQTCIDGLEEVDLALTNVIANYLKNSTEMTSNGLAIVSFFSKLTDSFSLRRLMSYDDR-- 240
           QQTCIDG ++    L + I +YLKNSTE+TSN LAIVS+ SK+  S  LRRL+++     
Sbjct: 181 QQTCIDGFDDAKAELKDKIVSYLKNSTELTSNSLAIVSWISKVVSSVKLRRLLTHPATTA 240

Query: 241 ---HSNG--DWARPILRKLAQK-DLRKQADIVVAKDKSGKYKTITEALNAVPDKSKKRTV 300
              +S G  +W  P  RKL +  DLRK+ADIVVA D SGKYKTI  AL AVPDKSKKRTV
Sbjct: 241 TASYSEGVPEWLHPRDRKLLESSDLRKKADIVVASDGSGKYKTIHAALKAVPDKSKKRTV 300

Query: 301 IYVKKGVYKENVEVVKNKWNVVMIGDGMTLT 322
           IYVKKGVY EN  V K KWNV+++GDG T T
Sbjct: 301 IYVKKGVYFENARVEKTKWNVLVVGDGSTAT 329

BLAST of Cp4.1LG20g05580 vs. TrEMBL
Match: V7BW13_PHAVU (Pectinesterase OS=Phaseolus vulgaris GN=PHAVU_005G012000g PE=4 SV=1)

HSP 1 Score: 305.8 bits (782), Expect = 6.3e-80
Identity = 165/325 (50.77%), Postives = 228/325 (70.15%), Query Frame = 1

Query: 1   MSLLRPYGKVDESDQMRLDASRRTRKRVIVIVLSSVVLVGVVVAVVFGTRNSSKNDESDN 60
           MS  + YGKVDE +Q  L+  R+TRKR+ +I LSS+VL GVV A VF    S+ + E  N
Sbjct: 1   MSSFKSYGKVDEHEQRVLETRRKTRKRITIIGLSSIVLAGVVFAAVFCILGSTNSSEGAN 60

Query: 61  HNEALSTSIKAVCDETLYPDTCKNAFGSLANSSHLDPGQIAKFSVQLALGELSRVADYLL 120
               ++ S+KAVCD TLY D+C ++ GS  +S  L P ++   S+++AL E+S+ ADYL 
Sbjct: 61  DAHTVTNSVKAVCDVTLYKDSCYSSLGSAVDSRQLQPQELFMLSMKVALSEVSKAADYLS 120

Query: 121 EHAITN--STDNKTILALRSCHELLELAIDHLNDSLSSSE-ITVLKAVDDLKTWLSSAAT 180
           +H + N  + DN+T  A ++C ELL+LA+DHLN SL+S E  ++L+  DDL+TWLS+A T
Sbjct: 121 DHGVFNGVAKDNRTKEAFKNCRELLDLAVDHLNSSLTSGEKFSLLEVFDDLETWLSAAGT 180

Query: 181 YQQTCIDGLEEVDLALTNVIANYLKNSTEMTSNGLAIVSFFSKLTDSFSLRRLMSYDDRH 240
           YQQTCI+G E+   A+   + +YLKNST+ TSN LAI+++ +K   + +LRRL+S   ++
Sbjct: 181 YQQTCIEGFEDGKEAIKKSVVSYLKNSTQFTSNSLAIITWINKAATTLNLRRLLSLHHQN 240

Query: 241 SNGDWARPILRK-LAQKDLRKQADIVVAKDKSGKYKTITEALNAVPDKSKKRTVIYVKKG 300
              +W     R+ L  +DLRK+ADIVVAKD SGKYK I++AL  VP++S KRTVIYVK+G
Sbjct: 241 EAPEWLPSNDRRLLLTEDLRKKADIVVAKDGSGKYKKISDALKHVPNESNKRTVIYVKRG 300

Query: 301 VYKENVEVVKNKWNVVMIGDGMTLT 322
           VY ENV V K KWNV++IGDGMT T
Sbjct: 301 VYYENVRVEKTKWNVMIIGDGMTST 325

BLAST of Cp4.1LG20g05580 vs. TrEMBL
Match: G7IVH6_MEDTR (Pectinesterase OS=Medicago truncatula GN=MTR_3g010780 PE=4 SV=1)

HSP 1 Score: 302.4 bits (773), Expect = 7.0e-79
Identity = 166/328 (50.61%), Postives = 230/328 (70.12%), Query Frame = 1

Query: 1   MSLLRPYGKVDESDQMRLDASRRTRKRVIVIVLSSVVLVGVVVAVVFGTRNSSKNDESDN 60
           MS  + YGKVDE DQM L+A R+TRKR+ +I LSS++ +GVV+A V G  NS KND+ +N
Sbjct: 1   MSFFKSYGKVDEHDQMMLEAKRKTRKRITIISLSSIIFIGVVIAAVLGVVNS-KNDDDNN 60

Query: 61  HN---EALSTSIKAVCDETLYPDTCKNAFGSLANSS-HLDPGQIAKFSVQLALGELSRVA 120
           +N    +++ S+KAVCD TLY D+C N+  S+  S   + P ++ K S+ +AL  +S+  
Sbjct: 61  NNASQNSVTNSMKAVCDVTLYKDSCYNSLSSIVGSGKEVQPEELFKLSINVALTHVSKAV 120

Query: 121 DYLLEHAITNST--DNKTILALRSCHELLELAIDHLNDSLSSSE--ITVLKAVDDLKTWL 180
           +Y  EH +      +++T  AL++C  LL+LAIDHLN++L++S    ++ +  DDL+TWL
Sbjct: 121 EYFNEHGVFKKLIENSRTNEALKNCRVLLDLAIDHLNNTLTASRENSSLHQVFDDLQTWL 180

Query: 181 SSAATYQQTCIDGLEEVDLALTNVIANYLKNSTEMTSNGLAIVSFFSKLTDSFSLRRLMS 240
           S+A TYQQTCI+G E+    L   + +YLKNSTE TSN LAI+++ +K  ++ +LRRLMS
Sbjct: 181 SAAGTYQQTCIEGFEDTKEQLKTSVTSYLKNSTEYTSNSLAIITYINKAINTLNLRRLMS 240

Query: 241 YDDRHSNGDWARPILRKL-AQKDLRKQADIVVAKDKSGKYKTITEALNAVPDKSKKRTVI 300
               +    W     RKL + KDLR +ADIVVAKD SGKYKTI++AL  VP+KSKKRT+I
Sbjct: 241 LPYENETPKWFHSKDRKLLSTKDLRSKADIVVAKDGSGKYKTISDALKHVPNKSKKRTLI 300

Query: 301 YVKKGVYKENVEVVKNKWNVVMIGDGMT 320
           YVKKG+Y ENV V K KWNV++IGDGMT
Sbjct: 301 YVKKGIYYENVRVEKTKWNVMIIGDGMT 327

BLAST of Cp4.1LG20g05580 vs. TAIR10
Match: AT3G10710.1 (AT3G10710.1 root hair specific 12)

HSP 1 Score: 263.8 bits (673), Expect = 1.4e-70
Identity = 150/320 (46.88%), Postives = 220/320 (68.75%), Query Frame = 1

Query: 6   PYGKVDESDQMRLDASRRTRKRVIVIVLSSVVLVGVVVAVVFGT---RNSSKNDESDNHN 65
           PYGKVDE + +RL+A R+TRK + +I +S V+L G+V+  VFGT   + S +  E++N+ 
Sbjct: 4   PYGKVDEREHVRLEARRKTRKNIAIIAVSLVILAGIVIGAVFGTMAHKKSPETVETNNNG 63

Query: 66  EALSTSIKAVCDETLYPDTCKNAFGSLANSSHLDPGQIAKFSVQLALGELSRVADYLLEH 125
           +++S S+KAVCD TL+ + C    GS  N+S L+P ++ +++V++ + E+S+  +     
Sbjct: 64  DSISVSVKAVCDVTLHKEKCFETLGSAPNASSLNPEELFRYAVKITIAEVSKAIN----- 123

Query: 126 AITNST-DNKTILALRSCHELLELAIDHLNDSLSSS---EITVLKAVDDLKTWLSSAATY 185
           A ++S  D K  + + +C ELL+L ID+LN++L+SS   ++TV + VDDL+TWLSSA TY
Sbjct: 124 AFSSSLGDEKNNITMNACAELLDLTIDNLNNTLTSSSNGDVTVPELVDDLRTWLSSAGTY 183

Query: 186 QQTCIDGLEEVDLALTNVIANYLKNSTEMTSNGLAIVSFFSKLTDSFSLRR-LMSYDDRH 245
           Q+TC++ L      +     ++LKNSTE+TSN LAI+++  K+ DSF LRR L++  D  
Sbjct: 184 QRTCVETLAP---DMRPFGESHLKNSTELTSNALAIITWLGKIADSFKLRRRLLTTADVE 243

Query: 246 SNGDWARPILRKLAQKDLRKQADIVVAKDKSGKYKTITEALNAVPDKSKKRTVIYVKKGV 305
            +    R   R L   DLRK ADIVVAKD SGKY+TI  AL  VP+KS+KRT+IYVKKGV
Sbjct: 244 VDFHAGR---RLLQSTDLRKVADIVVAKDGSGKYRTIKRALQDVPEKSEKRTIIYVKKGV 303

Query: 306 YKENVEVVKNKWNVVMIGDG 318
           Y ENV+V K  WNV+++GDG
Sbjct: 304 YFENVKVEKKMWNVIVVGDG 312

BLAST of Cp4.1LG20g05580 vs. TAIR10
Match: AT5G04960.1 (AT5G04960.1 Plant invertase/pectin methylesterase inhibitor superfamily)

HSP 1 Score: 260.0 bits (663), Expect = 2.0e-69
Identity = 153/324 (47.22%), Postives = 217/324 (66.98%), Query Frame = 1

Query: 7   YGKVDESDQMRLDASRRTRKRVIVIVLSSVVLVGVVVAVVFGT--RNSSKNDESDNHNEA 66
           YG++DE +Q +L+ASR+T+KR+ +I +SS+VLV +VV  V GT  R++SK   ++N+ E 
Sbjct: 4   YGRLDEHEQAKLEASRKTKKRIAIIAISSIVLVCIVVGAVVGTTARDNSKKPPTENNGEP 63

Query: 67  LSTSIKAVCDETLYPDTCKNAFGSLANSSHLDPGQIAKFSVQLALGELSRVADYLL--EH 126
           +S S+KA+CD TL+ + C    GS  N+S   P ++ K++V++ + ELS+V D     EH
Sbjct: 64  ISVSVKALCDVTLHKEKCFETLGSAPNASRSSPEELFKYAVKVTITELSKVLDGFSNGEH 123

Query: 127 AITNSTDNKTILALRSCHELLELAIDHLNDSLSSSEITVLKAVDDLKTWLSSAATYQQTC 186
                 DN T  A+ +C EL+ LA+D LN++++SS    LK  DDL+TWLSS  TYQ+TC
Sbjct: 124 M-----DNATSAAMGACVELIGLAVDQLNETMTSS----LKNFDDLRTWLSSVGTYQETC 183

Query: 187 IDGLEEVDL-ALTNVIANYLKNSTEMTSNGLAIVSFFSKLTDSFSLRRLMSYDDRHSNGD 246
           +D L E +  +LT    N+LKNSTEMTSN LAI+++  K+ D+   RR    +  ++   
Sbjct: 184 MDALVEANKPSLTTFGENHLKNSTEMTSNALAIITWLGKIADTVKFRRRRLLETGNAKVV 243

Query: 247 WAR-PIL---RKLAQKDLRKQADIVVAKDKSGKYKTITEALNAVPDKSKKRTVIYVKKGV 306
            A  P++   R L   DL+K+A IVVAKD SGKY+TI EAL  V +K++K T+IYVKKGV
Sbjct: 244 VADLPMMEGRRLLESGDLKKKATIVVAKDGSGKYRTIGEALAEVEEKNEKPTIIYVKKGV 303

Query: 307 YKENVEVVKNKWNVVMIGDGMTLT 322
           Y ENV V K KWNVVM+GDG + T
Sbjct: 304 YLENVRVEKTKWNVVMVGDGQSKT 318

BLAST of Cp4.1LG20g05580 vs. TAIR10
Match: AT1G53840.1 (AT1G53840.1 pectin methylesterase 1)

HSP 1 Score: 178.7 bits (452), Expect = 5.9e-45
Identity = 127/335 (37.91%), Postives = 191/335 (57.01%), Query Frame = 1

Query: 7   YGKVDESDQMRLDASRRTRKRVIVIVLSSVVLVGVVVAVVFGT---RNSSKNDESDNHNE 66
           YGKVDE+  + L   ++TRKR++++ +S VVL+ V++A V  T   +N +++  S     
Sbjct: 10  YGKVDEAQDLAL--KKKTRKRLLLLSISVVVLIAVIIAAVVATVVHKNKNESTPSPPPEL 69

Query: 67  ALSTSIKAVCDETLYPDTCKNAFGSLANSSHLDPGQIAKFSVQLALGELSRVADYLLEHA 126
             STS+KA+C  T +P++C ++   L +S+  DP  + K S+++ + EL  ++D L E  
Sbjct: 70  TPSTSLKAICSVTRFPESCISSISKLPSSNTTDPETLFKLSLKVIIDELDSISD-LPEKL 129

Query: 127 ITNSTDNKTILALRSCHELLELAIDHLNDSLSSSEITVLKA------VDDLKTWLSSAAT 186
              + D +   ALR C +L+E A+D LND++S+ +    K       ++DLKTWLS+  T
Sbjct: 130 SKETEDERIKSALRVCGDLIEDALDRLNDTVSAIDDEEKKKTLSSSKIEDLKTWLSATVT 189

Query: 187 YQQTCIDGLEEV--------DLALTNVIANYLKNSTEMTSNGLAIVS-FFSKLTDS---- 246
             +TC D L+E+        +  +T  + + +  STE TSN LAIVS   S L+D     
Sbjct: 190 DHETCFDSLDELKQNKTEYANSTITQNLKSAMSRSTEFTSNSLAIVSKILSALSDLGIPI 249

Query: 247 FSLRRLMSYDDRHSNG--DWARPILRKLAQKDLRKQADIVVAKDKSGKYKTITEALNAVP 306
              RRLMS+  + S     WAR   R+L Q    K  D+ VA D +G   T+ EA+  VP
Sbjct: 250 HRRRRLMSHHHQQSVDFEKWAR---RRLLQTAGLKP-DVTVAGDGTGDVLTVNEAVAKVP 309

Query: 307 DKSKKRTVIYVKKGVYKENVEVVKNKWNVVMIGDG 318
            KS K  VIYVK G Y ENV + K+KWNV++ GDG
Sbjct: 310 KKSLKMFVIYVKSGTYVENVVMDKSKWNVMIYGDG 337

BLAST of Cp4.1LG20g05580 vs. TAIR10
Match: AT5G49180.1 (AT5G49180.1 Plant invertase/pectin methylesterase inhibitor superfamily)

HSP 1 Score: 145.2 bits (365), Expect = 7.2e-35
Identity = 106/323 (32.82%), Postives = 170/323 (52.63%), Query Frame = 1

Query: 16  MRLDASRRTRKRVIVIVLSSVVLVGVVVAVVFGTRNSSKNDES-DNHNEALSTSIKAVCD 75
           M +D   + +K +I  V+++++++ VV   +  +RN+S +++      +  +T+++AVC 
Sbjct: 1   MGVDGELKKKKCIIAGVITALLVLMVVAVGITTSRNTSHSEKIVPVQIKTATTAVEAVCA 60

Query: 76  ETLYPDTCKNAFGSLANSSHLDPGQIAKFSVQLALGELS-RVADYLLEHAITNSTDNKTI 135
            T Y +TC N+    A+     P  + K    + +  +   +    +E     + D  T 
Sbjct: 61  PTDYKETCVNSLMK-ASPDSTQPLDLIKLGFNVTIRSIEDSIKKASVELTAKAANDKDTK 120

Query: 136 LALRSCHELLELAIDHLNDSLSS----SEITVLKAVDDLKTWLSSAATYQQTCIDGLEEV 195
            AL  C +L+  A D L   L +    S   +   V+DL+ WLS +  YQQTC+D  EE 
Sbjct: 121 GALELCEKLMNDATDDLKKCLDNFDGFSIPQIEDFVEDLRVWLSGSIAYQQTCMDTFEET 180

Query: 196 DLALTNVIANYLKNSTEMTSNGLAIVSFFSKLTDSFSL-----------RRLMSYDDRHS 255
           +  L+  +    K S E+TSNGLA+++  S L   F++           R+L+S +D   
Sbjct: 181 NSKLSQDMQKIFKTSRELTSNGLAMITNISNLLGEFNVTGVTGDLGKYARKLLSAEDGIP 240

Query: 256 NGDWARPILRKLAQKDLRKQADIVVAKDKSGKYKTITEALNAVPDKSKKRTVIYVKKGVY 315
           +  W  P  R+L       +A++VVA D SG+YKTI EALNAVP  ++K  VIY+K+GVY
Sbjct: 241 S--WVGPNTRRLMATKGGVKANVVVAHDGSGQYKTINEALNAVPKANQKPFVIYIKQGVY 300

Query: 316 KENVEVVKNKWNVVMIGDGMTLT 322
            E V+V K   +V  IGDG T T
Sbjct: 301 NEKVDVTKKMTHVTFIGDGPTKT 320

BLAST of Cp4.1LG20g05580 vs. TAIR10
Match: AT5G27870.1 (AT5G27870.1 Plant invertase/pectin methylesterase inhibitor superfamily)

HSP 1 Score: 140.6 bits (353), Expect = 1.8e-33
Identity = 104/312 (33.33%), Postives = 165/312 (52.88%), Query Frame = 1

Query: 19  DASRRTRKRVIVIVLSSVVLVGVVVAVVFGTRNSSKNDESDNHNEALSTSIKA---VCDE 78
           D   + +KR ++I +SSV+L+ +VVAV  G    S N   +  +E ++TS+KA   VC  
Sbjct: 7   DEDAKRKKRYVIISISSVLLISMVVAVTIGV---SVNKSDNAGDEEITTSVKAIKDVCAP 66

Query: 79  TLYPDTCKNAFGSLANSSHLDPGQIAKFSVQLALGELSRVADYLLEHAITNSTDNKTILA 138
           T Y +TC++     A  +  DP ++ K +    + ++S VA    +  I    D +  +A
Sbjct: 67  TDYKETCEDTLRKDAKDTS-DPLELVKTAFNATMKQISDVAKKS-QTMIELQKDPRAKMA 126

Query: 139 LRSCHELLELAIDHLNDSLSSSEITVLKAVDD----LKTWLSSAATYQQTCIDGLEEVDL 198
           L  C EL++ AI  L+ S           VD+    L+ WLS+  +++QTC+DG +    
Sbjct: 127 LDQCKELMDYAIGELSKSFEELGKFEFHKVDEALVKLRIWLSATISHEQTCLDGFQGTQG 186

Query: 199 ALTNVIANYLKNSTEMTSNGLAIVSFFSKLTDSFSL-----RRLMSYDDRHSNGDWARPI 258
                I   LK + ++T NGLA+V+  S       +     RRL+S +       W    
Sbjct: 187 NAGETIKKALKTAVQLTHNGLAMVTEMSNYLGQMQIPEMNSRRLLSQEFP----SWMDAR 246

Query: 259 LRKLAQKDLRK-QADIVVAKDKSGKYKTITEALNAVPDKSKKRTVIYVKKGVYKENVEVV 318
            R+L    + + + DIVVA+D SG+YKTI EALN VP K     V+++K+G+YKE V+V 
Sbjct: 247 ARRLLNAPMSEVKPDIVVAQDGSGQYKTINEALNFVPKKKNTTFVVHIKEGIYKEYVQVN 306

BLAST of Cp4.1LG20g05580 vs. NCBI nr
Match: gi|449435528|ref|XP_004135547.1| (PREDICTED: putative pectinesterase/pectinesterase inhibitor 24 [Cucumis sativus])

HSP 1 Score: 512.3 bits (1318), Expect = 6.4e-142
Identity = 261/322 (81.06%), Postives = 296/322 (91.93%), Query Frame = 1

Query: 1   MSLLRPYGKVDESDQMRLDASRRTRKRVIVIVLSSVVLVGVVVAVVFGTRNSSKN-DESD 60
           MS LRPYGKVDE+DQ+RLDA RRTR+R++VIVLSSVVLV VVVAVVFGTRN+S N D+S+
Sbjct: 1   MSFLRPYGKVDETDQLRLDARRRTRRRIVVIVLSSVVLVAVVVAVVFGTRNASNNNDDSN 60

Query: 61  NHNEALSTSIKAVCDETLYPDTCKNAFGSLANSSHLDPGQIAKFSVQLALGELSRVADYL 120
           N N +LSTSIKAVCD TLYPDTC+ AFG +ANSSHLDPGQI+K SVQLALGELS+VADY+
Sbjct: 61  NQNGSLSTSIKAVCDVTLYPDTCQKAFGPIANSSHLDPGQISKLSVQLALGELSKVADYI 120

Query: 121 LEHAITNSTDNKTILALRSCHELLELAIDHLNDSLSSSEITVLKAVDDLKTWLSSAATYQ 180
            +HAIT + DNKTILAL++CHELL+LA+DHLN SLSSS+IT+LKAVDDLKTW++SAATYQ
Sbjct: 121 FDHAITKTADNKTILALKNCHELLDLALDHLNISLSSSDITLLKAVDDLKTWITSAATYQ 180

Query: 181 QTCIDGLEEVDLALTNVIANYLKNSTEMTSNGLAIVSFFSKLTDSFSLRRLMSYDDRHSN 240
           QTCID L EVD AL +++AN+LKNSTE+TSNGLAIVSFFSKLTDS SLRRLMSY++  SN
Sbjct: 181 QTCIDDLAEVDPALADLVANFLKNSTELTSNGLAIVSFFSKLTDSLSLRRLMSYENHQSN 240

Query: 241 GDWARPILRKLAQKDLRKQADIVVAKDKSGKYKTITEALNAVPDKSKKRTVIYVKKGVYK 300
           GDWARPILRKLAQ D+RK ADIVVAKD+SGKYKTITEAL AVPDKSKKRTVIYVKKG+YK
Sbjct: 241 GDWARPILRKLAQSDIRKHADIVVAKDRSGKYKTITEALGAVPDKSKKRTVIYVKKGIYK 300

Query: 301 ENVEVVKNKWNVVMIGDGMTLT 322
           ENVEVVKNKWNVVM+GDGMT T
Sbjct: 301 ENVEVVKNKWNVVMVGDGMTAT 322

BLAST of Cp4.1LG20g05580 vs. NCBI nr
Match: gi|659068622|ref|XP_008445366.1| (PREDICTED: putative pectinesterase/pectinesterase inhibitor 24 [Cucumis melo])

HSP 1 Score: 511.5 bits (1316), Expect = 1.1e-141
Identity = 262/322 (81.37%), Postives = 295/322 (91.61%), Query Frame = 1

Query: 1   MSLLRPYGKVDESDQMRLDASRRTRKRVIVIVLSSVVLVGVVVAVVFGTRNSSKN-DESD 60
           MS LRPYGKVDE+DQ+RLDASRRTR+R++VIVLSSVVLV VVVAVVFGTRN+S N D+S+
Sbjct: 1   MSFLRPYGKVDETDQLRLDASRRTRRRIVVIVLSSVVLVAVVVAVVFGTRNASNNNDDSN 60

Query: 61  NHNEALSTSIKAVCDETLYPDTCKNAFGSLANSSHLDPGQIAKFSVQLALGELSRVADYL 120
           N N +LS SIKAVCD TLYPDTC+ AFG  ANSSHLDPGQI+K +VQLALGELS+VAD++
Sbjct: 61  NQNGSLSASIKAVCDVTLYPDTCQKAFGPFANSSHLDPGQISKLAVQLALGELSKVADHI 120

Query: 121 LEHAITNSTDNKTILALRSCHELLELAIDHLNDSLSSSEITVLKAVDDLKTWLSSAATYQ 180
            +HAITN+TD+KTILAL++CHELL+LA+DHLN +LSSS+IT+LKAVDDLKTWL+SAATYQ
Sbjct: 121 FDHAITNTTDDKTILALKNCHELLDLALDHLNITLSSSDITLLKAVDDLKTWLTSAATYQ 180

Query: 181 QTCIDGLEEVDLALTNVIANYLKNSTEMTSNGLAIVSFFSKLTDSFSLRRLMSYDDRHSN 240
           QTCIDGL EVD AL ++ AN+LKNSTE+TSNGLAIVSFFSKLTDS SLRRLMSY+DR SN
Sbjct: 181 QTCIDGLAEVDPALADLFANFLKNSTELTSNGLAIVSFFSKLTDSLSLRRLMSYEDRQSN 240

Query: 241 GDWARPILRKLAQKDLRKQADIVVAKDKSGKYKTITEALNAVPDKSKKRTVIYVKKGVYK 300
           GDWARPILRKLAQ D+RKQADIVVAKD SGKYKTITEAL AVPDKSKKRTVIYVKKG YK
Sbjct: 241 GDWARPILRKLAQSDIRKQADIVVAKDSSGKYKTITEALKAVPDKSKKRTVIYVKKGTYK 300

Query: 301 ENVEVVKNKWNVVMIGDGMTLT 322
           E VEVVKNKWNVVM+GDGM  T
Sbjct: 301 EKVEVVKNKWNVVMVGDGMNAT 322

BLAST of Cp4.1LG20g05580 vs. NCBI nr
Match: gi|645251887|ref|XP_008231878.1| (PREDICTED: probable pectinesterase/pectinesterase inhibitor 46 [Prunus mume])

HSP 1 Score: 316.6 bits (810), Expect = 5.1e-83
Identity = 181/331 (54.68%), Postives = 229/331 (69.18%), Query Frame = 1

Query: 1   MSLLRPYGKVDESDQMRLDASRRTRKRVIVIVLSSVVLVGVVVAVVFGTRNSSKNDESDN 60
           MS  + YGKV+E +Q RLDA R+TR+RV++I LSS+VLV VVVA V GT    KN+    
Sbjct: 1   MSSFKAYGKVNELEQSRLDARRKTRRRVVIISLSSIVLVCVVVAAVVGT-TIHKNNSGGK 60

Query: 61  HNEALSTSIKAVCDETLYPDTCKNAFGSLANSSHLDPGQIAKFSVQLALGELSRVADYLL 120
              +LSTS+KAVCD TLY D+C ++ G+L NS++  P  + K ++++A+ ELS+ A    
Sbjct: 61  QGSSLSTSVKAVCDVTLYQDSCYSSLGALVNSTNAKPEDLFKLAIRVAINELSKAASRFS 120

Query: 121 EHAITNSTDNKTI--LALRSCHELLELAIDHLNDSLSSSEITVLKAVDDLKTWLSSAATY 180
              I N T N T+   AL +C ELL LA+DHLNDSLS  + ++    DDLKTWLSS+ TY
Sbjct: 121 GDRILNGTSNDTLSKAALENCQELLRLALDHLNDSLSDDK-SLFDVFDDLKTWLSSSGTY 180

Query: 181 QQTCIDGLEEVDLALTNVIANYLKNSTEMTSNGLAIVSFFSKLTDSFSLRRLMSYDD--- 240
           QQTCIDG ++    L + IA+YLKNSTE+TSN LAIVS+ SK+  S  LRRL+++     
Sbjct: 181 QQTCIDGFDDAKAELKDKIASYLKNSTELTSNSLAIVSWISKVVSSVKLRRLLTHPATTA 240

Query: 241 --RHSNG--DWARPILRKLAQK-DLRKQADIVVAKDKSGKYKTITEALNAVPDKSKKRTV 300
              +S G  +W  P  RKL +  DLRK+ADIVVA D SGKYKTI  AL  VPDKSKKRTV
Sbjct: 241 TANYSEGVPEWLHPRDRKLLESSDLRKKADIVVASDGSGKYKTIHAALKVVPDKSKKRTV 300

Query: 301 IYVKKGVYKENVEVVKNKWNVVMIGDGMTLT 322
           IYVKKGVY ENV V K KWNV+++GDG T T
Sbjct: 301 IYVKKGVYFENVRVEKTKWNVLVVGDGSTAT 329

BLAST of Cp4.1LG20g05580 vs. NCBI nr
Match: gi|694422862|ref|XP_009339254.1| (PREDICTED: putative pectinesterase/pectinesterase inhibitor 24 [Pyrus x bretschneideri])

HSP 1 Score: 314.7 bits (805), Expect = 1.9e-82
Identity = 177/329 (53.80%), Postives = 236/329 (71.73%), Query Frame = 1

Query: 1   MSLLRPYGKVDESDQMRLDASRRTRKRVIVIVLSSVVLVGVVVAVVFGTRNSSKNDESDN 60
           MS  + YGKVDE +Q RLDA R+TRKRV +I LSS+VLV +VVA V GT   +K+   ++
Sbjct: 1   MSSFKSYGKVDEYEQARLDARRKTRKRVAIISLSSIVLVCIVVAAVVGTTVHNKSG-GNS 60

Query: 61  HNEALSTSIKAVCDETLYPDTCKNAFGSLANSSHLDPGQIAKFSVQLALGELSRVADYLL 120
               +STS+KAVCD TLY D+C ++ G L NS+++ P ++ K +VQ+A  EL++ A +  
Sbjct: 61  EGSGISTSVKAVCDVTLYQDSCYSSLGPLVNSNNVKPEELFKLAVQVANNELTKAASHFS 120

Query: 121 EHAITNST--DNKTILALRSCHELLELAIDHLNDSLSSSEITVLKAVDDLKTWLSSAATY 180
              + N+T  D K+  AL+ C +LL LA+DHLND+LSS+  +++   DDLKTWLSS+ TY
Sbjct: 121 ADGVLNATAKDRKSAAALKDCQDLLSLALDHLNDTLSSAN-SLVDVFDDLKTWLSSSGTY 180

Query: 181 QQTCIDGLEEVDLALTNVIANYLKNSTEMTSNGLAIVSFFSKLTDSFSLRRLMSYDD--- 240
           QQTCI+G +EV   L + +  YLKNSTE+TSN LAIVS+ SK+ +S  LRRL+++ D   
Sbjct: 181 QQTCIEGFDEVKGELKDKVIGYLKNSTELTSNSLAIVSWISKILNSVKLRRLLTHPDPTD 240

Query: 241 RHSNG--DWARPILRKLAQK-DLRKQADIVVAKDKSGKYKTITEALNAVPDKSKKRTVIY 300
            +S G  +W     RKL Q  DLRK+ADIVVA+D SGKYKTI +AL AVP+KSKKRT+IY
Sbjct: 241 SYSQGVPEWLHRKDRKLLQSSDLRKKADIVVARDGSGKYKTIHDALKAVPNKSKKRTIIY 300

Query: 301 VKKGVYKENVEVVKNKWNVVMIGDGMTLT 322
           VKKGVY  NV V K+KWNV+++GDG T T
Sbjct: 301 VKKGVYYGNVRVEKSKWNVLVVGDGSTAT 327

BLAST of Cp4.1LG20g05580 vs. NCBI nr
Match: gi|590569436|ref|XP_007011069.1| (Root hair specific 12 [Theobroma cacao])

HSP 1 Score: 313.9 bits (803), Expect = 3.3e-82
Identity = 176/326 (53.99%), Postives = 229/326 (70.25%), Query Frame = 1

Query: 2   SLLRPYGKVDESDQMRLDASRRTRKRVIVIVLSSVVLVGVVVAVVFGTRNSSKNDESDNH 61
           ++ R YGKV+E+DQ RL A R+TR+R+ +I LS +VL  +VVA VFG+R S  + ++  +
Sbjct: 32  NMFRAYGKVNEADQARLLARRKTRRRIAIIGLSFIVLAAIVVAAVFGSRGSGGDSKNGGN 91

Query: 62  N--EALSTSIKAVCDETLYPDTCKNAFGSLANSSHLDPGQIAKFSVQLALGELSRVADYL 121
              + +S+SIKAVCD TLY DTC ++   +ANSS L P  I K S+++A+ ELS+ + Y 
Sbjct: 92  GGVQPISSSIKAVCDVTLYKDTCYDSLAPMANSSQLQPEDIFKLSMKVAIAELSKASQYF 151

Query: 122 LEHAITNST-DNKTILALRSCHELLELAIDHLNDSLSSS-EITVLKAVDDLKTWLSSAAT 181
            ++ I     D  +I AL +C ELL LAIDHLN SLSSS E++V++AVDDL+TWLSSA+T
Sbjct: 152 SKNGIFKDVADKMSIAALENCGELLSLAIDHLNSSLSSSGEVSVIQAVDDLRTWLSSAST 211

Query: 182 YQQTCIDGLEEVDLALTNVIANYLKNSTEMTSNGLAIVSFFSKLTDSFSLRRLMSYDDRH 241
           YQQTCID  EE+       + ++LK S+E+TSN LAI+++ SK+    +LRRLMS    H
Sbjct: 212 YQQTCIDAFEELKGDTKASVHDHLKYSSELTSNSLAIITWISKVASVLNLRRLMSSSPNH 271

Query: 242 SNGDWARPILRKLAQKD--LRKQADIVVAKDKSGKYKTITEALNAVPDKSKKRTVIYVKK 301
              +W     RKL Q    L+K+ADI+VAKD SGKYKTI+ AL AVPDKSKKR VIYVKK
Sbjct: 272 EEPEWFHVKDRKLLQSSDLLKKKADIIVAKDGSGKYKTISAALKAVPDKSKKRIVIYVKK 331

Query: 302 GVYKENVEVVKNKWNVVMIGDGMTLT 322
           GVY ENV V K KWNV MIGDGM  T
Sbjct: 332 GVYSENVSVEKKKWNVTMIGDGMEST 357

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
PME24_ARATH2.5e-6946.88Putative pectinesterase/pectinesterase inhibitor 24 OS=Arabidopsis thaliana GN=P... [more]
PME46_ARATH3.6e-6847.22Probable pectinesterase/pectinesterase inhibitor 46 OS=Arabidopsis thaliana GN=P... [more]
PME3_PHAVU1.0e-4336.61Pectinesterase 3 OS=Phaseolus vulgaris GN=MPE3 PE=2 SV=1[more]
PME1_ARATH1.0e-4337.91Pectinesterase 1 OS=Arabidopsis thaliana GN=PME1 PE=1 SV=1[more]
PME58_ARATH1.3e-3332.82Probable pectinesterase/pectinesterase inhibitor 58 OS=Arabidopsis thaliana GN=P... [more]
Match NameE-valueIdentityDescription
A0A0A0LY08_CUCSA4.4e-14281.06Pectinesterase OS=Cucumis sativus GN=Csa_1G538170 PE=4 SV=1[more]
A0A061FYJ8_THECC2.3e-8253.99Pectinesterase OS=Theobroma cacao GN=TCM_045262 PE=4 SV=1[more]
M5XPH8_PRUPE6.7e-8254.08Pectinesterase OS=Prunus persica GN=PRUPE_ppa024156mg PE=4 SV=1[more]
V7BW13_PHAVU6.3e-8050.77Pectinesterase OS=Phaseolus vulgaris GN=PHAVU_005G012000g PE=4 SV=1[more]
G7IVH6_MEDTR7.0e-7950.61Pectinesterase OS=Medicago truncatula GN=MTR_3g010780 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT3G10710.11.4e-7046.88 root hair specific 12[more]
AT5G04960.12.0e-6947.22 Plant invertase/pectin methylesterase inhibitor superfamily[more]
AT1G53840.15.9e-4537.91 pectin methylesterase 1[more]
AT5G49180.17.2e-3532.82 Plant invertase/pectin methylesterase inhibitor superfamily[more]
AT5G27870.11.8e-3333.33 Plant invertase/pectin methylesterase inhibitor superfamily[more]
Match NameE-valueIdentityDescription
gi|449435528|ref|XP_004135547.1|6.4e-14281.06PREDICTED: putative pectinesterase/pectinesterase inhibitor 24 [Cucumis sativus][more]
gi|659068622|ref|XP_008445366.1|1.1e-14181.37PREDICTED: putative pectinesterase/pectinesterase inhibitor 24 [Cucumis melo][more]
gi|645251887|ref|XP_008231878.1|5.1e-8354.68PREDICTED: probable pectinesterase/pectinesterase inhibitor 46 [Prunus mume][more]
gi|694422862|ref|XP_009339254.1|1.9e-8253.80PREDICTED: putative pectinesterase/pectinesterase inhibitor 24 [Pyrus x bretschn... [more]
gi|590569436|ref|XP_007011069.1|3.3e-8253.99Root hair specific 12 [Theobroma cacao][more]
The following terms have been associated with this gene:
Vocabulary: Molecular Function
TermDefinition
GO:0004857enzyme inhibitor activity
GO:0030599pectinesterase activity
Vocabulary: Biological Process
TermDefinition
GO:0042545cell wall modification
Vocabulary: Cellular Component
TermDefinition
GO:0005618cell wall
Vocabulary: INTERPRO
TermDefinition
IPR018040Pectinesterase_Tyr_AS
IPR012334Pectin_lyas_fold
IPR011050Pectin_lyase_fold/virulence
IPR006501Pectinesterase_inhib_dom
IPR000070Pectinesterase_cat
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0042545 cell wall modification
biological_process GO:0043086 negative regulation of catalytic activity
biological_process GO:0045490 pectin catabolic process
biological_process GO:0005982 starch metabolic process
biological_process GO:0005985 sucrose metabolic process
biological_process GO:0008150 biological_process
cellular_component GO:0005618 cell wall
cellular_component GO:0005576 extracellular region
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0005575 cellular_component
molecular_function GO:0045330 aspartyl esterase activity
molecular_function GO:0004857 enzyme inhibitor activity
molecular_function GO:0030599 pectinesterase activity

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG20g05580.1Cp4.1LG20g05580.1mRNA


Analysis Name: InterPro Annotations of Cucurbita pepo
Date Performed: 2017-12-02
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000070Pectinesterase, catalyticPFAMPF01095Pectinesterasecoord: 260..321
score: 1.8
IPR006501Pectinesterase inhibitor domainGENE3DG3DSA:1.20.140.40coord: 66..217
score: 5.3
IPR006501Pectinesterase inhibitor domainPFAMPF04043PMEIcoord: 68..214
score: 5.2
IPR006501Pectinesterase inhibitor domainSMARTSM00856PMEI_2coord: 64..214
score: 3.5
IPR006501Pectinesterase inhibitor domainTIGRFAMsTIGR01614TIGR01614coord: 37..215
score: 1.2
IPR006501Pectinesterase inhibitor domainunknownSSF101148Plant invertase/pectin methylesterase inhibitorcoord: 68..219
score: 5.1
IPR011050Pectin lyase fold/virulence factorunknownSSF51126Pectin lyase-likecoord: 258..322
score: 3.08
IPR012334Pectin lyase foldGENE3DG3DSA:2.160.20.10coord: 258..322
score: 3.5
IPR018040Pectinesterase, Tyr active sitePROSITEPS00800PECTINESTERASE_1coord: 285..304
scor
NoneNo IPR availablePANTHERPTHR31707FAMILY NOT NAMEDcoord: 25..321
score: 2.2E
NoneNo IPR availablePANTHERPTHR31707:SF48PECTINESTERASE/PECTINESTERASE INHIBITOR 24-RELATEDcoord: 25..321
score: 2.2E

The following gene(s) are paralogous to this gene:

None