Csa1G538170 (gene) Cucumber (Chinese Long) v2

BLAST of Csa1G538170 vs. Swiss-Prot
Match: PME24_ARATH (Putative pectinesterase/pectinesterase inhibitor 24 OS=Arabidopsis thaliana GN=PME24 PE=3 SV=1)

HSP 1 Score: 609.0 bits (1569), Expect = 5.3e-173
Identity = 312/568 (54.93%), Postives = 399/568 (70.25%), Query Frame = 1

Query: 6   PYGKVDETDQLRLDARRRTRRRIVVIVLSSVVLVAVVVAVVFGT--RNASNNNDDSNNQN 65
           PYGKVDE + +RL+ARR+TR+ I +I +S V+L  +V+  VFGT     S    ++NN  
Sbjct: 4   PYGKVDEREHVRLEARRKTRKNIAIIAVSLVILAGIVIGAVFGTMAHKKSPETVETNNNG 63

Query: 66  GSLSTSIKAVCDVTLYPDTCQKAFGPIANSSHLDPGQISKLSVQLALGELSKVADYIFDH 125
            S+S S+KAVCDVTL+ + C +  G   N+S L+P ++ + +V++ + E+SK  +     
Sbjct: 64  DSISVSVKAVCDVTLHKEKCFETLGSAPNASSLNPEELFRYAVKITIAEVSKAINAFS-- 123

Query: 126 AITKTADNKTILALKNCHELLDLALDHLNISLSSS---DITLLKAVDDLKTWITSAATYQ 185
             +   D K  + +  C ELLDL +D+LN +L+SS   D+T+ + VDDL+TW++SA TYQ
Sbjct: 124 --SSLGDEKNNITMNACAELLDLTIDNLNNTLTSSSNGDVTVPELVDDLRTWLSSAGTYQ 183

Query: 186 QTCIDDLAEVDPALADLVANFLKNSTELTSNGLAIVSFFSKLTDSLSLRR-LMSYENHQS 245
           +TC++ LA   P +     + LKNSTELTSN LAI+++  K+ DS  LRR L++  + + 
Sbjct: 184 RTCVETLA---PDMRPFGESHLKNSTELTSNALAIITWLGKIADSFKLRRRLLTTADVEV 243

Query: 246 NGDWARPILRKLAQSDIRKHADIVVAKDRSGKYKTITEALGAVPDKSKKRTVIYVKKGIY 305
           +    R   R L  +D+RK ADIVVAKD SGKY+TI  AL  VP+KS+KRT+IYVKKG+Y
Sbjct: 244 DFHAGR---RLLQSTDLRKVADIVVAKDGSGKYRTIKRALQDVPEKSEKRTIIYVKKGVY 303

Query: 306 KENVEVVKNKWNVVMVGDGMTATIVSGNLNVVDGTPTFSTATFAAKGKGFIAIDMGFINT 365
            ENV+V K  WNV++VGDG + +IVSG LNV+DGTPTF TATFA  GKGF+A DMGFINT
Sbjct: 304 FENVKVEKKMWNVIVVGDGESKSIVSGRLNVIDGTPTFKTATFAVFGKGFMARDMGFINT 363

Query: 366 AGPSKHQAVALMSTSDQSIFYRCEMNAYQDTLYAHSNRQFYRECKVYGTVDFIFGNSAVV 425
           AGPSKHQAVALM ++D + FYRC MNAYQDTLY H+ RQFYREC + GTVDFIFGNSA V
Sbjct: 364 AGPSKHQAVALMVSADLTAFYRCTMNAYQDTLYVHAQRQFYRECTIIGTVDFIFGNSASV 423

Query: 426 LQNCTIAPRLPLLGQKNTITAQGRFDPNQNTGISIQGSLIQPFNDLKTTETYLGRPWKNY 485
           LQ+C I PR P+ GQ+NTITAQGR DPN NTGISI    I P  DL    T+LGRPWKN+
Sbjct: 424 LQSCRILPRRPMKGQQNTITAQGRTDPNMNTGISIHRCNISPLGDLTDVMTFLGRPWKNF 483

Query: 486 STTVFMQSNLGRLIHPTGWLPWIGTSAPDTIFYAEFQNYGPGSSTSKRVKWKGVKNIDTK 545
           STTV M S L   I   GWLPW G SAPDTIFY E++N GPG+ST  RVKWKG++ + TK
Sbjct: 484 STTVIMDSYLHGFIDRKGWLPWTGDSAPDTIFYGEYKNTGPGASTKNRVKWKGLRFLSTK 543

Query: 546 TAKKFTVSSFIDGKDWISKAQVPFTATL 568
            A +FTV  FIDG  W+   +VPF + L
Sbjct: 544 EANRFTVKPFIDGGRWLPATKVPFRSGL 561

BLAST of Csa1G538170 vs. Swiss-Prot
Match: PME46_ARATH (Probable pectinesterase/pectinesterase inhibitor 46 OS=Arabidopsis thaliana GN=PME46 PE=2 SV=1)

HSP 1 Score: 590.9 bits (1522), Expect = 1.5e-167
Identity = 301/566 (53.18%), Postives = 397/566 (70.14%), Query Frame = 1

Query: 7   YGKVDETDQLRLDARRRTRRRIVVIVLSSVVLVAVVVAVVFGTRNASNNNDDSNNQNGS- 66
           YG++DE +Q +L+A R+T++RI +I +SS+VLV +VV  V GT    N+       NG  
Sbjct: 4   YGRLDEHEQAKLEASRKTKKRIAIIAISSIVLVCIVVGAVVGTTARDNSKKPPTENNGEP 63

Query: 67  LSTSIKAVCDVTLYPDTCQKAFGPIANSSHLDPGQISKLSVQLALGELSKVADYIFDHAI 126
           +S S+KA+CDVTL+ + C +  G   N+S   P ++ K +V++ + ELSKV D   +   
Sbjct: 64  ISVSVKALCDVTLHKEKCFETLGSAPNASRSSPEELFKYAVKVTITELSKVLDGFSNG-- 123

Query: 127 TKTADNKTILALKNCHELLDLALDHLNISLSSSDITLLKAVDDLKTWITSAATYQQTCID 186
            +  DN T  A+  C EL+ LA+D LN +++SS    LK  DDL+TW++S  TYQ+TC+D
Sbjct: 124 -EHMDNATSAAMGACVELIGLAVDQLNETMTSS----LKNFDDLRTWLSSVGTYQETCMD 183

Query: 187 DLAEVD-PALADLVANFLKNSTELTSNGLAIVSFFSKLTDSLSLRRLMSYENHQSNGDWA 246
            L E + P+L     N LKNSTE+TSN LAI+++  K+ D++  RR    E   +    A
Sbjct: 184 ALVEANKPSLTTFGENHLKNSTEMTSNALAIITWLGKIADTVKFRRRRLLETGNAKVVVA 243

Query: 247 R-PIL---RKLAQSDIRKHADIVVAKDRSGKYKTITEALGAVPDKSKKRTVIYVKKGIYK 306
             P++   R L   D++K A IVVAKD SGKY+TI EAL  V +K++K T+IYVKKG+Y 
Sbjct: 244 DLPMMEGRRLLESGDLKKKATIVVAKDGSGKYRTIGEALAEVEEKNEKPTIIYVKKGVYL 303

Query: 307 ENVEVVKNKWNVVMVGDGMTATIVSGNLNVVDGTPTFSTATFAAKGKGFIAIDMGFINTA 366
           ENV V K KWNVVMVGDG + TIVS  LN +DGTPTF TATFA  GKGF+A DMGFINTA
Sbjct: 304 ENVRVEKTKWNVVMVGDGQSKTIVSAGLNFIDGTPTFETATFAVFGKGFMARDMGFINTA 363

Query: 367 GPSKHQAVALMSTSDQSIFYRCEMNAYQDTLYAHSNRQFYRECKVYGTVDFIFGNSAVVL 426
           GP+KHQAVALM ++D S+FY+C M+A+QDT+YAH+ RQFYR+C + GTVDFIFGN+AVV 
Sbjct: 364 GPAKHQAVALMVSADLSVFYKCTMDAFQDTMYAHAQRQFYRDCVILGTVDFIFGNAAVVF 423

Query: 427 QNCTIAPRLPLLGQKNTITAQGRFDPNQNTGISIQGSLIQPFNDLKTTETYLGRPWKNYS 486
           Q C I PR P+ GQ+NTITAQGR DPNQNTGISI    I+P ++L   +T+LGRPWK++S
Sbjct: 424 QKCEILPRRPMKGQQNTITAQGRKDPNQNTGISIHNCTIKPLDNLTDIQTFLGRPWKDFS 483

Query: 487 TTVFMQSNLGRLIHPTGWLPWIGTSAPDTIFYAEFQNYGPGSSTSKRVKWKGVK-NIDTK 546
           TTV M+S + + I+P GWLPW G +APDTIFYAE+ N GPG+ST  RVKW+G+K ++  K
Sbjct: 484 TTVIMKSFMDKFINPKGWLPWTGDTAPDTIFYAEYLNSGPGASTKNRVKWQGLKTSLTKK 543

Query: 547 TAKKFTVSSFIDGKDWISKAQVPFTA 566
            A KFTV  FIDG +W+   +VPF +
Sbjct: 544 EANKFTVKPFIDGNNWLPATKVPFNS 562

BLAST of Csa1G538170 vs. Swiss-Prot
Match: PME3_PHAVU (Pectinesterase 3 OS=Phaseolus vulgaris GN=MPE3 PE=2 SV=1)

HSP 1 Score: 494.2 bits (1271), Expect = 1.9e-138
Identity = 269/583 (46.14%), Postives = 371/583 (63.64%), Query Frame = 1

Query: 7   YGKVDETDQLRLDARRRTRRRIVVIVLSSVVLVAVVVAVVFGTRNASNNNDDSNNQNG-- 66
           YGKV+E +Q   +  ++TR+R+++I +SS+VL+AV++A V G    + N++ S + +   
Sbjct: 10  YGKVNELEQQAYE--KKTRKRLIIIAVSSIVLIAVIIAAVAGVVIHNRNSESSPSSDSVP 69

Query: 67  ----SLSTSIKAVCDVTLYPDTCQKAFGPIANSSHLDPGQISKLSVQLALGELSKVADYI 126
               S + S+KAVCD T YP +C  +   +  S+  DP  + KLS+++A+ ELS     +
Sbjct: 70  QTELSPAASLKAVCDTTRYPSSCFSSISSLPESNTTDPELLFKLSLRVAIDELSSFPSKL 129

Query: 127 FDHAITKTADNKTILALKNCHELLDLALDHLNISLSS-----SDITLLKAVDDLKTWITS 186
             +A     D +   A+  C  +   ALD LN S+S+       I    +V +++TW+++
Sbjct: 130 RANA---EQDARLQKAIDVCSSVFGDALDRLNDSISALGTVAGRIASSASVSNVETWLSA 189

Query: 187 AATYQQTCIDDLAEVDPALA----DLVANFLKNSTELTSNGLAIVS----FFSKLTDSLS 246
           A T Q TC+D + E++   A      +   ++NSTE  SN LAIV+      S+    + 
Sbjct: 190 ALTDQDTCLDAVGELNSTAARGALQEIETAMRNSTEFASNSLAIVTKILGLLSRFETPIH 249

Query: 247 LRRLMSYENHQSNGDWARPILRKLAQSDIRKHA-DIVVAKDRSGKYKTITEALGAVPDKS 306
            RRL+ +       +W     R+L +        D VVAKD SG++KTI EAL  V  KS
Sbjct: 250 HRRLLGFP------EWLGAAERRLLEEKNNDSTPDAVVAKDGSGQFKTIGEALKLVKKKS 309

Query: 307 KKRTVIYVKKGIYKENVEVVKNKWNVVMVGDGMTATIVSGNLNVVDGTPTFSTATFAAKG 366
           ++R  +YVK+G Y EN+++ KN WNV++ GDG   T V G+ N +DGTPTF TATFA KG
Sbjct: 310 EERFSVYVKEGRYVENIDLDKNTWNVMIYGDGKDKTFVVGSRNFMDGTPTFETATFAVKG 369

Query: 367 KGFIAIDMGFINTAGPSKHQAVALMSTSDQSIFYRCEMNAYQDTLYAHSNRQFYRECKVY 426
           KGFIA D+GF+N AG SKHQAVAL S SD+S+F+RC  + +QDTLYAHSNRQFYR+C + 
Sbjct: 370 KGFIAKDIGFVNNAGASKHQAVALRSGSDRSVFFRCSFDGFQDTLYAHSNRQFYRDCDIT 429

Query: 427 GTVDFIFGNSAVVLQNCTIAPRLPLLGQKNTITAQGRFDPNQNTGISIQGSLIQPFNDLK 486
           GT+DFIFGN+AVV Q+C I PR PL  Q NTITAQG+ DPNQNTGI IQ S I PF +  
Sbjct: 430 GTIDFIFGNAAVVFQSCKIMPRQPLPNQFNTITAQGKKDPNQNTGIIIQKSTITPFGNNL 489

Query: 487 TTETYLGRPWKNYSTTVFMQSNLGRLIHPTGWLPWI-GTSAPDTIFYAEFQNYGPGSSTS 546
           T  TYLGRPWK++STTV MQS++G L++P GW+ W+     P TIFYAE+QN GPG+  S
Sbjct: 490 TAPTYLGRPWKDFSTTVIMQSDIGALLNPVGWMSWVPNVEPPTTIFYAEYQNSGPGADVS 549

Query: 547 KRVKWKGVK-NIDTKTAKKFTVSSFIDGKDWISKAQVPFTATL 568
           +RVKW G K  I  + A++FTV SFI G +W+  A V F +TL
Sbjct: 550 QRVKWAGYKPTITDRNAEEFTVQSFIQGPEWLPNAAVQFDSTL 581

BLAST of Csa1G538170 vs. Swiss-Prot
Match: PME1_ARATH (Pectinesterase 1 OS=Arabidopsis thaliana GN=PME1 PE=1 SV=1)

HSP 1 Score: 461.8 bits (1187), Expect = 1.1e-128
Identity = 255/573 (44.50%), Postives = 360/573 (62.83%), Query Frame = 1

Query: 7   YGKVDETDQLRLDARRRTRRRIVVIVLSSVVLVAVVVAVVFGTRNASNNNDDSNNQNGSL 66
           YGKVDE   L L  +++TR+R++++ +S VVL+AV++A V  T    N N+ + +    L
Sbjct: 10  YGKVDEAQDLAL--KKKTRKRLLLLSISVVVLIAVIIAAVVATVVHKNKNESTPSPPPEL 69

Query: 67  --STSIKAVCDVTLYPDTCQKAFGPIANSSHLDPGQISKLSVQLALGELSKVADYIFDHA 126
             STS+KA+C VT +P++C  +   + +S+  DP  + KLS+++ + EL  ++D + +  
Sbjct: 70  TPSTSLKAICSVTRFPESCISSISKLPSSNTTDPETLFKLSLKVIIDELDSISD-LPEKL 129

Query: 127 ITKTADNKTILALKNCHELLDLALDHLNISLSSSDITLLKA------VDDLKTWITSAAT 186
             +T D +   AL+ C +L++ ALD LN ++S+ D    K       ++DLKTW+++  T
Sbjct: 130 SKETEDERIKSALRVCGDLIEDALDRLNDTVSAIDDEEKKKTLSSSKIEDLKTWLSATVT 189

Query: 187 YQQTCIDDLAEV--------DPALADLVANFLKNSTELTSNGLAIVSFFSKLTDSLSL-- 246
             +TC D L E+        +  +   + + +  STE TSN LAIVS        L +  
Sbjct: 190 DHETCFDSLDELKQNKTEYANSTITQNLKSAMSRSTEFTSNSLAIVSKILSALSDLGIPI 249

Query: 247 ---RRLMSYENHQSNGDWARPILRKLAQSDIRKHADIVVAKDRSGKYKTITEALGAVPDK 306
              RRLMS+ +HQ + D+ +   R+L Q+   K  D+ VA D +G   T+ EA+  VP K
Sbjct: 250 HRRRRLMSH-HHQQSVDFEKWARRRLLQTAGLK-PDVTVAGDGTGDVLTVNEAVAKVPKK 309

Query: 307 SKKRTVIYVKKGIYKENVEVVKNKWNVVMVGDGMTATIVSGNLNVVDGTPTFSTATFAAK 366
           S K  VIYVK G Y ENV + K+KWNV++ GDG   TI+SG+ N VDGTPT+ TATFA +
Sbjct: 310 SLKMFVIYVKSGTYVENVVMDKSKWNVMIYGDGKGKTIISGSKNFVDGTPTYETATFAIQ 369

Query: 367 GKGFIAIDMGFINTAGPSKHQAVALMSTSDQSIFYRCEMNAYQDTLYAHSNRQFYRECKV 426
           GKGFI  D+G INTAG +KHQAVA  S SD S++Y+C  + +QDTLY HSNRQFYR+C V
Sbjct: 370 GKGFIMKDIGIINTAGAAKHQAVAFRSGSDFSVYYQCSFDGFQDTLYPHSNRQFYRDCDV 429

Query: 427 YGTVDFIFGNSAVVLQNCTIAPRLPLLGQKNTITAQGRFDPNQNTGISIQGSLIQPFNDL 486
            GT+DFIFG++AVV Q C I PR PL  Q NTITAQG+ DPNQ++G+SIQ   I   N  
Sbjct: 430 TGTIDFIFGSAAVVFQGCKIMPRQPLSNQFNTITAQGKKDPNQSSGMSIQRCTISA-NGN 489

Query: 487 KTTETYLGRPWKNYSTTVFMQSNLGRLIHPTGWLPWI-GTSAPDTIFYAEFQNYGPGSST 546
               TYLGRPWK +STTV M++ +G ++ P+GW+ W+ G   P +I Y E++N GPGS  
Sbjct: 490 VIAPTYLGRPWKEFSTTVIMETVIGAVVRPSGWMSWVSGVDPPASIVYGEYKNTGPGSDV 549

Query: 547 SKRVKWKGVKNI-DTKTAKKFTVSSFIDGKDWI 557
           ++RVKW G K +     A KFTV++ + G DWI
Sbjct: 550 TQRVKWAGYKPVMSDAEAAKFTVATLLHGADWI 576

BLAST of Csa1G538170 vs. Swiss-Prot
Match: PME1_CITSI (Pectinesterase 1 OS=Citrus sinensis GN=PECS-1.1 PE=2 SV=1)

HSP 1 Score: 405.2 bits (1040), Expect = 1.2e-111
Identity = 234/565 (41.42%), Postives = 331/565 (58.58%), Query Frame = 1

Query: 23  RTRRRIVVIVLSSVVLVAVVVAVVFGTRNASNNNDDSNNQNGSLSTSIKAVCDVTLYPDT 82
           + ++++ + + +++++VA V+ +V G  +  N+ D+ N  + ++   +K+ C  T YPD 
Sbjct: 25  KKKKKLFLALFATLLVVAAVIGIVAGVNSRKNSGDNGNEPHHAI---LKSSCSSTRYPDL 84

Query: 83  CQKAFGPIANSSHLDPGQISKLSVQLALGELSKVADYIFDHAITKTAD--NKTILALKNC 142
           C  A   +  +S     Q   + + L +   +   +Y     + K  +   +  +AL +C
Sbjct: 85  CFSAIAAVPEASKKVTSQKDVIEMSLNITTTAVEHNYFGIQKLLKRTNLTKREKVALHDC 144

Query: 143 HELLDLALDHLNISLSSSDI-----TLLKAVDDLKTWITSAATYQQTCIDDLAEVDPA-- 202
            E +D  LD L+ ++   +      +L +  DDLKT +++A T Q TC+D  +  D    
Sbjct: 145 LETIDETLDELHKAVEDLEEYPNKKSLSQHADDLKTLMSAAMTNQGTCLDGFSHDDANKH 204

Query: 203 LADLVANFLKNSTELTSNGLAIVSFFSKLTDSLSLR----RLMSYENHQSNG--DWARPI 262
           + D +++   +  ++ SN LA++   +  TD + +R    R ++ E    +G   W  P 
Sbjct: 205 VRDALSDGQVHVEKMCSNALAMIKNMTD-TDMMIMRTSNNRKLTEETSTVDGWPAWLSPG 264

Query: 263 LRKLAQSDIRKHADIVVAKDRSGKYKTITEALGAVPDKSKKRTVIYVKKGIYKENVEVVK 322
            R+L QS      + VVA D SG +KT+  A+ A P    KR +I +K G+Y+ENVEV K
Sbjct: 265 DRRLLQSS-SVTPNAVVAADGSGNFKTVAAAVAAAPQGGTKRYIIRIKAGVYRENVEVTK 324

Query: 323 NKWNVVMVGDGMTATIVSGNLNVVDGTPTFSTATFAAKGKGFIAIDMGFINTAGPSKHQA 382
              N++ +GDG T TI++G+ NVVDG+ TF +AT A  G+GF+A D+ F NTAGPSKHQA
Sbjct: 325 KHKNIMFIGDGRTRTIITGSRNVVDGSTTFKSATAAVVGEGFLARDITFQNTAGPSKHQA 384

Query: 383 VALMSTSDQSIFYRCEMNAYQDTLYAHSNRQFYRECKVYGTVDFIFGNSAVVLQNCTIAP 442
           VAL   +D S FY C+M AYQDTLY HSNRQF+  C + GTVDFIFGN+A VLQNC I  
Sbjct: 385 VALRVGADLSAFYNCDMLAYQDTLYVHSNRQFFVNCLIAGTVDFIFGNAAAVLQNCDIHA 444

Query: 443 RLPLLGQKNTITAQGRFDPNQNTGISIQGSLIQPFNDLKTTE----TYLGRPWKNYSTTV 502
           R P  GQKN +TAQGR DPNQNTGI IQ S I   +DLK  +    TYLGRPWK YS TV
Sbjct: 445 RKPNSGQKNMVTAQGRTDPNQNTGIVIQKSRIGATSDLKPVQGSFPTYLGRPWKEYSRTV 504

Query: 503 FMQSNLGRLIHPTGWLPWIGTSAPDTIFYAEFQNYGPGSSTSKRVKWKGVKNIDTKT-AK 562
            MQS++  LIHP GW  W G  A +T+FY E QN G G+ TS RVKWKG + I + T A+
Sbjct: 505 IMQSSITDLIHPAGWHEWDGNFALNTLFYGEHQNSGAGAGTSGRVKWKGFRVITSATEAQ 564

Query: 563 KFTVSSFIDGKDWISKAQVPFTATL 568
            FT  SFI G  W+     PF+  L
Sbjct: 565 AFTPGSFIAGSSWLGSTGFPFSLGL 584

BLAST of Csa1G538170 vs. TrEMBL
Match: A0A0A0LY08_CUCSA (Pectinesterase OS=Cucumis sativus GN=Csa_1G538170 PE=4 SV=1)

HSP 1 Score: 1127.5 bits (2915), Expect = 0.0e+00
Identity = 567/567 (100.00%), Postives = 567/567 (100.00%), Query Frame = 1

Query: 1   MSFLRPYGKVDETDQLRLDARRRTRRRIVVIVLSSVVLVAVVVAVVFGTRNASNNNDDSN 60
           MSFLRPYGKVDETDQLRLDARRRTRRRIVVIVLSSVVLVAVVVAVVFGTRNASNNNDDSN
Sbjct: 1   MSFLRPYGKVDETDQLRLDARRRTRRRIVVIVLSSVVLVAVVVAVVFGTRNASNNNDDSN 60

Query: 61  NQNGSLSTSIKAVCDVTLYPDTCQKAFGPIANSSHLDPGQISKLSVQLALGELSKVADYI 120
           NQNGSLSTSIKAVCDVTLYPDTCQKAFGPIANSSHLDPGQISKLSVQLALGELSKVADYI
Sbjct: 61  NQNGSLSTSIKAVCDVTLYPDTCQKAFGPIANSSHLDPGQISKLSVQLALGELSKVADYI 120

Query: 121 FDHAITKTADNKTILALKNCHELLDLALDHLNISLSSSDITLLKAVDDLKTWITSAATYQ 180
           FDHAITKTADNKTILALKNCHELLDLALDHLNISLSSSDITLLKAVDDLKTWITSAATYQ
Sbjct: 121 FDHAITKTADNKTILALKNCHELLDLALDHLNISLSSSDITLLKAVDDLKTWITSAATYQ 180

Query: 181 QTCIDDLAEVDPALADLVANFLKNSTELTSNGLAIVSFFSKLTDSLSLRRLMSYENHQSN 240
           QTCIDDLAEVDPALADLVANFLKNSTELTSNGLAIVSFFSKLTDSLSLRRLMSYENHQSN
Sbjct: 181 QTCIDDLAEVDPALADLVANFLKNSTELTSNGLAIVSFFSKLTDSLSLRRLMSYENHQSN 240

Query: 241 GDWARPILRKLAQSDIRKHADIVVAKDRSGKYKTITEALGAVPDKSKKRTVIYVKKGIYK 300
           GDWARPILRKLAQSDIRKHADIVVAKDRSGKYKTITEALGAVPDKSKKRTVIYVKKGIYK
Sbjct: 241 GDWARPILRKLAQSDIRKHADIVVAKDRSGKYKTITEALGAVPDKSKKRTVIYVKKGIYK 300

Query: 301 ENVEVVKNKWNVVMVGDGMTATIVSGNLNVVDGTPTFSTATFAAKGKGFIAIDMGFINTA 360
           ENVEVVKNKWNVVMVGDGMTATIVSGNLNVVDGTPTFSTATFAAKGKGFIAIDMGFINTA
Sbjct: 301 ENVEVVKNKWNVVMVGDGMTATIVSGNLNVVDGTPTFSTATFAAKGKGFIAIDMGFINTA 360

Query: 361 GPSKHQAVALMSTSDQSIFYRCEMNAYQDTLYAHSNRQFYRECKVYGTVDFIFGNSAVVL 420
           GPSKHQAVALMSTSDQSIFYRCEMNAYQDTLYAHSNRQFYRECKVYGTVDFIFGNSAVVL
Sbjct: 361 GPSKHQAVALMSTSDQSIFYRCEMNAYQDTLYAHSNRQFYRECKVYGTVDFIFGNSAVVL 420

Query: 421 QNCTIAPRLPLLGQKNTITAQGRFDPNQNTGISIQGSLIQPFNDLKTTETYLGRPWKNYS 480
           QNCTIAPRLPLLGQKNTITAQGRFDPNQNTGISIQGSLIQPFNDLKTTETYLGRPWKNYS
Sbjct: 421 QNCTIAPRLPLLGQKNTITAQGRFDPNQNTGISIQGSLIQPFNDLKTTETYLGRPWKNYS 480

Query: 481 TTVFMQSNLGRLIHPTGWLPWIGTSAPDTIFYAEFQNYGPGSSTSKRVKWKGVKNIDTKT 540
           TTVFMQSNLGRLIHPTGWLPWIGTSAPDTIFYAEFQNYGPGSSTSKRVKWKGVKNIDTKT
Sbjct: 481 TTVFMQSNLGRLIHPTGWLPWIGTSAPDTIFYAEFQNYGPGSSTSKRVKWKGVKNIDTKT 540

Query: 541 AKKFTVSSFIDGKDWISKAQVPFTATL 568
           AKKFTVSSFIDGKDWISKAQVPFTATL
Sbjct: 541 AKKFTVSSFIDGKDWISKAQVPFTATL 567

BLAST of Csa1G538170 vs. TrEMBL
Match: M5XPH8_PRUPE (Pectinesterase OS=Prunus persica GN=PRUPE_ppa024156mg PE=4 SV=1)

HSP 1 Score: 697.2 bits (1798), Expect = 1.7e-197
Identity = 354/577 (61.35%), Postives = 431/577 (74.70%), Query Frame = 1

Query: 1   MSFLRPYGKVDETDQLRLDARRRTRRRIVVIVLSSVVLVAVVVAVVFGTRNASNNNDDSN 60
           MS  + YGKV+E +Q RLDARR+TRRR+V+I LSS+VLV VVVA V GT    NN+    
Sbjct: 1   MSSFKAYGKVNELEQSRLDARRKTRRRVVIISLSSIVLVCVVVAAVVGTTIHKNNS--GG 60

Query: 61  NQNGSLSTSIKAVCDVTLYPDTCQKAFGPIANSSHLDPGQISKLSVQLALGELSKVADYI 120
               SLSTS+KAVCDVTLY D+C  + G + NS++  P  + KL++++A+ ELSK A   
Sbjct: 61  KPGSSLSTSVKAVCDVTLYQDSCYSSLGALVNSTNAKPEDLFKLAIRVAINELSKAASRF 120

Query: 121 FDHAITKTADNKTI--LALKNCHELLDLALDHLNISLSSSDITLLKAVDDLKTWITSAAT 180
               I     + T+   AL+NC ELL LALDHLN SLS  D +L    DDLKTW++S+ T
Sbjct: 121 SGDGILNGTSHDTLSKAALENCQELLSLALDHLNDSLSD-DKSLFDVFDDLKTWLSSSGT 180

Query: 181 YQQTCIDDLAEVDPALADLVANFLKNSTELTSNGLAIVSFFSKLTDSLSLRRLMSYENHQ 240
           YQQTCID   +    L D + ++LKNSTELTSN LAIVS+ SK+  S+ LRRL+++    
Sbjct: 181 YQQTCIDGFDDAKAELKDKIVSYLKNSTELTSNSLAIVSWISKVVSSVKLRRLLTHPATT 240

Query: 241 SNG-------DWARPILRKLAQS-DIRKHADIVVAKDRSGKYKTITEALGAVPDKSKKRT 300
           +         +W  P  RKL +S D+RK ADIVVA D SGKYKTI  AL AVPDKSKKRT
Sbjct: 241 ATASYSEGVPEWLHPRDRKLLESSDLRKKADIVVASDGSGKYKTIHAALKAVPDKSKKRT 300

Query: 301 VIYVKKGIYKENVEVVKNKWNVVMVGDGMTATIVSGNLNVVDGTPTFSTATFAAKGKGFI 360
           VIYVKKG+Y EN  V K KWNV++VGDG TATIVS   NV+DGTPTFS+ATFA  GKGFI
Sbjct: 301 VIYVKKGVYFENARVEKTKWNVLVVGDGSTATIVSAGRNVIDGTPTFSSATFAVFGKGFI 360

Query: 361 AIDMGFINTAGPSKHQAVALMSTSDQSIFYRCEMNAYQDTLYAHSNRQFYRECKVYGTVD 420
           A D+GF NTAG +KHQAVALMST+DQS+FYRC ++A+QDTLYAHSNRQFYREC +YGTVD
Sbjct: 361 ARDIGFRNTAGAAKHQAVALMSTADQSVFYRCHIDAFQDTLYAHSNRQFYRECNIYGTVD 420

Query: 421 FIFGNSAVVLQNCTIAPRLPLLGQKNTITAQGRFDPNQNTGISIQGSLIQPFNDLKTTET 480
           FIFGNSAVVLQNC I P+ P+ GQ+NTITAQG+ DPNQNTGISIQ   I P+ DL + +T
Sbjct: 421 FIFGNSAVVLQNCNIMPKRPMAGQQNTITAQGKIDPNQNTGISIQNCSISPYGDLSSVQT 480

Query: 481 YLGRPWKNYSTTVFMQSNLGRLIHPTGWLPWIGTSAPDTIFYAEFQNYGPGSSTSKRVKW 540
           YLGRPWKNYSTTV+MQ+ +G LI+P+GWLPW+G +APDTIFY+EFQN+GPGSST  RVKW
Sbjct: 481 YLGRPWKNYSTTVYMQTFMGSLINPSGWLPWVGNTAPDTIFYSEFQNFGPGSSTRNRVKW 540

Query: 541 KGVKNIDTKTAKKFTVSSFIDGKDWISKAQVPFTATL 568
           KG++ I++KTA KFTV S + G  WIS A V + + L
Sbjct: 541 KGLRTINSKTAGKFTVHSLLQGNKWISDASVTYKSNL 574

BLAST of Csa1G538170 vs. TrEMBL
Match: A0A061FYJ8_THECC (Pectinesterase OS=Theobroma cacao GN=TCM_045262 PE=4 SV=1)

HSP 1 Score: 695.7 bits (1794), Expect = 4.8e-197
Identity = 356/574 (62.02%), Postives = 432/574 (75.26%), Query Frame = 1

Query: 2   SFLRPYGKVDETDQLRLDARRRTRRRIVVIVLSSVVLVAVVVAVVFGTRNASNNNDDSNN 61
           +  R YGKV+E DQ RL ARR+TRRRI +I LS +VL A+VVA VFG+R +    D  N 
Sbjct: 32  NMFRAYGKVNEADQARLLARRKTRRRIAIIGLSFIVLAAIVVAAVFGSRGSGG--DSKNG 91

Query: 62  QNGS---LSTSIKAVCDVTLYPDTCQKAFGPIANSSHLDPGQISKLSVQLALGELSKVAD 121
            NG    +S+SIKAVCDVTLY DTC  +  P+ANSS L P  I KLS+++A+ ELSK + 
Sbjct: 92  GNGGVQPISSSIKAVCDVTLYKDTCYDSLAPMANSSQLQPEDIFKLSMKVAIAELSKASQ 151

Query: 122 YIFDHAITK-TADNKTILALKNCHELLDLALDHLNISLSSS-DITLLKAVDDLKTWITSA 181
           Y   + I K  AD  +I AL+NC ELL LA+DHLN SLSSS ++++++AVDDL+TW++SA
Sbjct: 152 YFSKNGIFKDVADKMSIAALENCGELLSLAIDHLNSSLSSSGEVSVIQAVDDLRTWLSSA 211

Query: 182 ATYQQTCIDDLAEVDPALADLVANFLKNSTELTSNGLAIVSFFSKLTDSLSLRRLMSYEN 241
           +TYQQTCID   E+       V + LK S+ELTSN LAI+++ SK+   L+LRRLMS   
Sbjct: 212 STYQQTCIDAFEELKGDTKASVHDHLKYSSELTSNSLAIITWISKVASVLNLRRLMSSSP 271

Query: 242 HQSNGDWARPILRKLAQSD--IRKHADIVVAKDRSGKYKTITEALGAVPDKSKKRTVIYV 301
           +    +W     RKL QS   ++K ADI+VAKD SGKYKTI+ AL AVPDKSKKR VIYV
Sbjct: 272 NHEEPEWFHVKDRKLLQSSDLLKKKADIIVAKDGSGKYKTISAALKAVPDKSKKRIVIYV 331

Query: 302 KKGIYKENVEVVKNKWNVVMVGDGMTATIVSGNLNVVDGTPTFSTATFAAKGKGFIAIDM 361
           KKG+Y ENV V K KWNV M+GDGM +T+VSG+LN VDGTPTFSTATFA  GKGF+A D+
Sbjct: 332 KKGVYSENVSVEKKKWNVTMIGDGMESTVVSGSLNYVDGTPTFSTATFAVFGKGFVARDI 391

Query: 362 GFINTAGPSKHQAVALMSTSDQSIFYRCEMNAYQDTLYAHSNRQFYRECKVYGTVDFIFG 421
           GF+NTAGP KHQAVALMST+DQS+FYRC   A+QDTLYAHSNRQFYREC + GTVDFIFG
Sbjct: 392 GFVNTAGPQKHQAVALMSTADQSVFYRCRFEAFQDTLYAHSNRQFYRECNIIGTVDFIFG 451

Query: 422 NSAVVLQNCTIAPRLPLLGQKNTITAQGRFDPNQNTGISIQGSLIQPFNDLKTT-ETYLG 481
           NSAVV QNC I PR PL  Q+NTITAQG+ DPNQNTGI+IQ   I P+  L T+  TYLG
Sbjct: 452 NSAVVFQNCNILPRQPLPNQQNTITAQGKVDPNQNTGIAIQNCTILPYGKLDTSLRTYLG 511

Query: 482 RPWKNYSTTVFMQSNLGRLIHPTGWLPWIGTSAPDTIFYAEFQNYGPGSSTSKRVKWKGV 541
           RPWKNYSTT+ M S+LG +IHPTGWLPW GT+APDTIFY+E++N GPGSST  RVKWKG+
Sbjct: 512 RPWKNYSTTILMHSSLGSVIHPTGWLPWSGTTAPDTIFYSEYKNTGPGSSTKDRVKWKGL 571

Query: 542 KNIDTKTAKKFTVSSFIDGKDWISKAQVPFTATL 568
           ++I  K AKKFTV  F+ G  WIS   V + ++L
Sbjct: 572 RSITDKEAKKFTVKEFLHGDKWISDTGVSYKSSL 603

BLAST of Csa1G538170 vs. TrEMBL
Match: B9RPW2_RICCO (Pectinesterase OS=Ricinus communis GN=RCOM_1542240 PE=4 SV=1)

HSP 1 Score: 687.6 bits (1773), Expect = 1.3e-194
Identity = 349/571 (61.12%), Postives = 431/571 (75.48%), Query Frame = 1

Query: 1   MSFLRPYGKVDETDQLRLDARRRTRRRIVVIVLSSVVLVAVVVAVVFGTRNASNNNDDSN 60
           MS  + YGKVDE +Q  L+ARRRTR+RI +I LSS++L A+VVA V GT  +  N+++  
Sbjct: 1   MSSFKAYGKVDEAEQATLEARRRTRKRITIISLSSILLAAIVVAAVVGTHASGGNSNNGG 60

Query: 61  NQNGSLSTSIKAVCDVTLYPDTCQKAFGPIANSSHLDPGQISKLSVQLALGELSKVADYI 120
           +Q   +STS+KAVCDVTLY D+C  +  P A  ++L P ++ KLS+Q+AL E+SK + Y 
Sbjct: 61  DQAKPISTSVKAVCDVTLYKDSCYSSLAPFAKPNNLQPEELFKLSIQVALDEISKASQYF 120

Query: 121 FDHA--ITKTADNKTI-LALKNCHELLDLALDHLNISLSSS-DITLLKAVDDLKTWITSA 180
            ++   +    DN  I  ALK+C +LLDLA+D LN SLSS+ D++L+   DD ++W+++A
Sbjct: 121 INNGQFLGGLNDNNMINAALKDCQDLLDLAIDRLNSSLSSANDVSLIDVADDFRSWLSAA 180

Query: 181 ATYQQTCIDDLAEVDPALADLVANF-LKNSTELTSNGLAIVSFFSKLTDSLSLRRLMSYE 240
            +YQQTCID L E +  L     N+ LKN+TELTSN LAI+++  K+  S+ +RRLMSY 
Sbjct: 181 GSYQQTCIDGLKEAN--LKSTAQNYYLKNTTELTSNSLAIITWIYKIASSVKMRRLMSYA 240

Query: 241 NHQSNG--DWARPILRKLAQS-DIRKHADIVVAKDRSGKYKTITEALGAVPDKSKKRTVI 300
            H       W     RKL QS D++K A+ VVAKD SGKYKTI++AL AVPDKSKKR +I
Sbjct: 241 EHDKVNLPRWLHQNDRKLLQSNDLKKKANAVVAKDGSGKYKTISDALKAVPDKSKKRFII 300

Query: 301 YVKKGIYKENVEVVKNKWNVVMVGDGMTATIVSGNLNVVDGTPTFSTATFAAKGKGFIAI 360
           YVKKGIY ENV V K KWNVV+VGDGM ATIVSG+LN VDGTPTFSTATFA  GKGFIA 
Sbjct: 301 YVKKGIYTENVRVEKPKWNVVIVGDGMNATIVSGSLNFVDGTPTFSTATFAVFGKGFIAR 360

Query: 361 DMGFINTAGPSKHQAVALMSTSDQSIFYRCEMNAYQDTLYAHSNRQFYRECKVYGTVDFI 420
           DMGF NTAGP KHQAVALMST+D S+FYRC  +A+QDTLYAHSNRQFYREC +YGTVDFI
Sbjct: 361 DMGFRNTAGPIKHQAVALMSTADMSVFYRCSFDAFQDTLYAHSNRQFYRECNIYGTVDFI 420

Query: 421 FGNSAVVLQNCTIAPRLPLLGQKNTITAQGRFDPNQNTGISIQGSLIQPFNDLKTTETYL 480
           FGNSAVV+QNC I PR  + GQKNTITAQGRFDPNQNTGISIQ   I PF++L + +T+L
Sbjct: 421 FGNSAVVIQNCNILPRRTMPGQKNTITAQGRFDPNQNTGISIQNCTILPFDNLTSIQTFL 480

Query: 481 GRPWKNYSTTVFMQSNLGRLIHPTGWLPWIGTSAPDTIFYAEFQNYGPGSSTSKRVKWKG 540
           GRPWKNYSTTV+M S +G LI P+GWLPW G +AP TIFYAEFQN+GPGSST  RVKWKG
Sbjct: 481 GRPWKNYSTTVYMHSMMGSLIDPSGWLPWTGNTAPPTIFYAEFQNFGPGSSTKNRVKWKG 540

Query: 541 VKNIDTKTAKKFTVSSFIDGKDWISKAQVPF 564
           +KNI  K A KFT ++F+ G  WI ++ V +
Sbjct: 541 LKNITYKLASKFTANAFLQGDKWIPESGVSY 569

BLAST of Csa1G538170 vs. TrEMBL
Match: I1M0H1_SOYBN (Pectinesterase OS=Glycine max GN=GLYMA_13G186100 PE=4 SV=1)

HSP 1 Score: 685.3 bits (1767), Expect = 6.5e-194
Identity = 342/579 (59.07%), Postives = 430/579 (74.27%), Query Frame = 1

Query: 1   MSFLRPYGKVDETDQLRLDARRRTRRRIVVIVLSSVVLVAVVVAVVFGT--RNASNNNDD 60
           MS  + YGKVDE++Q RL+ARR+T +R+ +I LS++VL+ VV A VFGT   N S+NND 
Sbjct: 1   MSSFKSYGKVDESEQARLEARRKTGKRVTIIALSTIVLIGVVCAAVFGTVAHNNSDNNDG 60

Query: 61  SNNQNGS-LSTSIKAVCDVTLYPDTCQKAFGPIANSSHLDPGQISKLSVQLALGELSKVA 120
            N+ +   LS S+K+VCD+TLY   C  + GP+ +S  + P ++  LS+++AL E S+  
Sbjct: 61  VNSNSAPFLSNSVKSVCDLTLYKGACYSSIGPLVHSGQVRPEKLFLLSIEVALAEASRAV 120

Query: 121 DYIFDHAITK---TADNKTILALKNCHELLDLALDHLNISLSSSD-ITLLKAVDDLKTWI 180
           +Y  +  +       DNKT+   KNC +LL LA+DHLN SL+S    +LL  ++DL+TW+
Sbjct: 121 EYFSEKGVFNGLINVDNKTMEGFKNCKDLLGLAVDHLNSSLASGGKSSLLDVLEDLRTWL 180

Query: 181 TSAATYQQTCIDDLAEVDPALADLVANFLKNSTELTSNGLAIVSFFSKLTDSLSLRRLMS 240
           ++A TYQQTCID   E   AL   V N LKNSTE TSN LAIV++ +K   +++LRRL+S
Sbjct: 181 SAAGTYQQTCIDGFGEAGEALKTSVVNNLKNSTEFTSNSLAIVTWLNKAASTVNLRRLLS 240

Query: 241 ----YENHQSNGDWARPILRKLAQSD--IRKHADIVVAKDRSGKYKTITEALGAVPDKSK 300
               + +H     W     RKL Q D  +++ ADIVVAKD SGK+KTIT AL  VP+KS 
Sbjct: 241 TTLPHHHHMVEPKWLHSKDRKLIQKDDNLKRKADIVVAKDGSGKFKTITAALKHVPEKSD 300

Query: 301 KRTVIYVKKGIYKENVEVVKNKWNVVMVGDGMTATIVSGNLNVVDGTPTFSTATFAAKGK 360
           KRTVIYVKKG+Y ENV V K KWNV+++GDGM ATIVSG+LN VDGTPTFSTATFA  GK
Sbjct: 301 KRTVIYVKKGVYYENVRVEKTKWNVMIIGDGMNATIVSGSLNFVDGTPTFSTATFAVFGK 360

Query: 361 GFIAIDMGFINTAGPSKHQAVALMSTSDQSIFYRCEMNAYQDTLYAHSNRQFYRECKVYG 420
            FIA DMGF NTAGP KHQAVALM+++DQ+++YRC+++A+QD+LYAHSNRQFYREC +YG
Sbjct: 361 NFIARDMGFRNTAGPQKHQAVALMTSADQAVYYRCQIDAFQDSLYAHSNRQFYRECNIYG 420

Query: 421 TVDFIFGNSAVVLQNCTIAPRLPLLGQKNTITAQGRFDPNQNTGISIQGSLIQPFNDLKT 480
           TVDFIFGNSAVVLQNC I PR+P+ GQ+NTITAQG+ DPN NTGISIQ   I PF DL +
Sbjct: 421 TVDFIFGNSAVVLQNCNIFPRVPMQGQQNTITAQGKTDPNMNTGISIQSCNIAPFGDLSS 480

Query: 481 TETYLGRPWKNYSTTVFMQSNLGRLIHPTGWLPWIGTSAPDTIFYAEFQNYGPGSSTSKR 540
            +TYLGRPWKNYSTTVFMQS LG  IHP GWLPW+G SAPDTIFYAEFQN GPGSST  R
Sbjct: 481 VKTYLGRPWKNYSTTVFMQSTLGSFIHPNGWLPWVGDSAPDTIFYAEFQNVGPGSSTKNR 540

Query: 541 VKWKGVKNIDTKTAKKFTVSSFIDGKDWISKAQVPFTAT 567
           VKWKG+K I  K A  FTV++F+ G+ WI+ +  PF ++
Sbjct: 541 VKWKGLKTITKKQASMFTVNAFLSGEKWITASGAPFKSS 579

BLAST of Csa1G538170 vs. TAIR10
Match: AT3G10710.1 (AT3G10710.1 root hair specific 12)

HSP 1 Score: 609.0 bits (1569), Expect = 3.0e-174
Identity = 312/568 (54.93%), Postives = 399/568 (70.25%), Query Frame = 1

Query: 6   PYGKVDETDQLRLDARRRTRRRIVVIVLSSVVLVAVVVAVVFGT--RNASNNNDDSNNQN 65
           PYGKVDE + +RL+ARR+TR+ I +I +S V+L  +V+  VFGT     S    ++NN  
Sbjct: 4   PYGKVDEREHVRLEARRKTRKNIAIIAVSLVILAGIVIGAVFGTMAHKKSPETVETNNNG 63

Query: 66  GSLSTSIKAVCDVTLYPDTCQKAFGPIANSSHLDPGQISKLSVQLALGELSKVADYIFDH 125
            S+S S+KAVCDVTL+ + C +  G   N+S L+P ++ + +V++ + E+SK  +     
Sbjct: 64  DSISVSVKAVCDVTLHKEKCFETLGSAPNASSLNPEELFRYAVKITIAEVSKAINAFS-- 123

Query: 126 AITKTADNKTILALKNCHELLDLALDHLNISLSSS---DITLLKAVDDLKTWITSAATYQ 185
             +   D K  + +  C ELLDL +D+LN +L+SS   D+T+ + VDDL+TW++SA TYQ
Sbjct: 124 --SSLGDEKNNITMNACAELLDLTIDNLNNTLTSSSNGDVTVPELVDDLRTWLSSAGTYQ 183

Query: 186 QTCIDDLAEVDPALADLVANFLKNSTELTSNGLAIVSFFSKLTDSLSLRR-LMSYENHQS 245
           +TC++ LA   P +     + LKNSTELTSN LAI+++  K+ DS  LRR L++  + + 
Sbjct: 184 RTCVETLA---PDMRPFGESHLKNSTELTSNALAIITWLGKIADSFKLRRRLLTTADVEV 243

Query: 246 NGDWARPILRKLAQSDIRKHADIVVAKDRSGKYKTITEALGAVPDKSKKRTVIYVKKGIY 305
           +    R   R L  +D+RK ADIVVAKD SGKY+TI  AL  VP+KS+KRT+IYVKKG+Y
Sbjct: 244 DFHAGR---RLLQSTDLRKVADIVVAKDGSGKYRTIKRALQDVPEKSEKRTIIYVKKGVY 303

Query: 306 KENVEVVKNKWNVVMVGDGMTATIVSGNLNVVDGTPTFSTATFAAKGKGFIAIDMGFINT 365
            ENV+V K  WNV++VGDG + +IVSG LNV+DGTPTF TATFA  GKGF+A DMGFINT
Sbjct: 304 FENVKVEKKMWNVIVVGDGESKSIVSGRLNVIDGTPTFKTATFAVFGKGFMARDMGFINT 363

Query: 366 AGPSKHQAVALMSTSDQSIFYRCEMNAYQDTLYAHSNRQFYRECKVYGTVDFIFGNSAVV 425
           AGPSKHQAVALM ++D + FYRC MNAYQDTLY H+ RQFYREC + GTVDFIFGNSA V
Sbjct: 364 AGPSKHQAVALMVSADLTAFYRCTMNAYQDTLYVHAQRQFYRECTIIGTVDFIFGNSASV 423

Query: 426 LQNCTIAPRLPLLGQKNTITAQGRFDPNQNTGISIQGSLIQPFNDLKTTETYLGRPWKNY 485
           LQ+C I PR P+ GQ+NTITAQGR DPN NTGISI    I P  DL    T+LGRPWKN+
Sbjct: 424 LQSCRILPRRPMKGQQNTITAQGRTDPNMNTGISIHRCNISPLGDLTDVMTFLGRPWKNF 483

Query: 486 STTVFMQSNLGRLIHPTGWLPWIGTSAPDTIFYAEFQNYGPGSSTSKRVKWKGVKNIDTK 545
           STTV M S L   I   GWLPW G SAPDTIFY E++N GPG+ST  RVKWKG++ + TK
Sbjct: 484 STTVIMDSYLHGFIDRKGWLPWTGDSAPDTIFYGEYKNTGPGASTKNRVKWKGLRFLSTK 543

Query: 546 TAKKFTVSSFIDGKDWISKAQVPFTATL 568
            A +FTV  FIDG  W+   +VPF + L
Sbjct: 544 EANRFTVKPFIDGGRWLPATKVPFRSGL 561

BLAST of Csa1G538170 vs. TAIR10
Match: AT5G04960.1 (AT5G04960.1 Plant invertase/pectin methylesterase inhibitor superfamily)

HSP 1 Score: 590.9 bits (1522), Expect = 8.4e-169
Identity = 301/566 (53.18%), Postives = 397/566 (70.14%), Query Frame = 1

Query: 7   YGKVDETDQLRLDARRRTRRRIVVIVLSSVVLVAVVVAVVFGTRNASNNNDDSNNQNGS- 66
           YG++DE +Q +L+A R+T++RI +I +SS+VLV +VV  V GT    N+       NG  
Sbjct: 4   YGRLDEHEQAKLEASRKTKKRIAIIAISSIVLVCIVVGAVVGTTARDNSKKPPTENNGEP 63

Query: 67  LSTSIKAVCDVTLYPDTCQKAFGPIANSSHLDPGQISKLSVQLALGELSKVADYIFDHAI 126
           +S S+KA+CDVTL+ + C +  G   N+S   P ++ K +V++ + ELSKV D   +   
Sbjct: 64  ISVSVKALCDVTLHKEKCFETLGSAPNASRSSPEELFKYAVKVTITELSKVLDGFSNG-- 123

Query: 127 TKTADNKTILALKNCHELLDLALDHLNISLSSSDITLLKAVDDLKTWITSAATYQQTCID 186
            +  DN T  A+  C EL+ LA+D LN +++SS    LK  DDL+TW++S  TYQ+TC+D
Sbjct: 124 -EHMDNATSAAMGACVELIGLAVDQLNETMTSS----LKNFDDLRTWLSSVGTYQETCMD 183

Query: 187 DLAEVD-PALADLVANFLKNSTELTSNGLAIVSFFSKLTDSLSLRRLMSYENHQSNGDWA 246
            L E + P+L     N LKNSTE+TSN LAI+++  K+ D++  RR    E   +    A
Sbjct: 184 ALVEANKPSLTTFGENHLKNSTEMTSNALAIITWLGKIADTVKFRRRRLLETGNAKVVVA 243

Query: 247 R-PIL---RKLAQSDIRKHADIVVAKDRSGKYKTITEALGAVPDKSKKRTVIYVKKGIYK 306
             P++   R L   D++K A IVVAKD SGKY+TI EAL  V +K++K T+IYVKKG+Y 
Sbjct: 244 DLPMMEGRRLLESGDLKKKATIVVAKDGSGKYRTIGEALAEVEEKNEKPTIIYVKKGVYL 303

Query: 307 ENVEVVKNKWNVVMVGDGMTATIVSGNLNVVDGTPTFSTATFAAKGKGFIAIDMGFINTA 366
           ENV V K KWNVVMVGDG + TIVS  LN +DGTPTF TATFA  GKGF+A DMGFINTA
Sbjct: 304 ENVRVEKTKWNVVMVGDGQSKTIVSAGLNFIDGTPTFETATFAVFGKGFMARDMGFINTA 363

Query: 367 GPSKHQAVALMSTSDQSIFYRCEMNAYQDTLYAHSNRQFYRECKVYGTVDFIFGNSAVVL 426
           GP+KHQAVALM ++D S+FY+C M+A+QDT+YAH+ RQFYR+C + GTVDFIFGN+AVV 
Sbjct: 364 GPAKHQAVALMVSADLSVFYKCTMDAFQDTMYAHAQRQFYRDCVILGTVDFIFGNAAVVF 423

Query: 427 QNCTIAPRLPLLGQKNTITAQGRFDPNQNTGISIQGSLIQPFNDLKTTETYLGRPWKNYS 486
           Q C I PR P+ GQ+NTITAQGR DPNQNTGISI    I+P ++L   +T+LGRPWK++S
Sbjct: 424 QKCEILPRRPMKGQQNTITAQGRKDPNQNTGISIHNCTIKPLDNLTDIQTFLGRPWKDFS 483

Query: 487 TTVFMQSNLGRLIHPTGWLPWIGTSAPDTIFYAEFQNYGPGSSTSKRVKWKGVK-NIDTK 546
           TTV M+S + + I+P GWLPW G +APDTIFYAE+ N GPG+ST  RVKW+G+K ++  K
Sbjct: 484 TTVIMKSFMDKFINPKGWLPWTGDTAPDTIFYAEYLNSGPGASTKNRVKWQGLKTSLTKK 543

Query: 547 TAKKFTVSSFIDGKDWISKAQVPFTA 566
            A KFTV  FIDG +W+   +VPF +
Sbjct: 544 EANKFTVKPFIDGNNWLPATKVPFNS 562

BLAST of Csa1G538170 vs. TAIR10
Match: AT1G53840.1 (AT1G53840.1 pectin methylesterase 1)

HSP 1 Score: 461.8 bits (1187), Expect = 5.9e-130
Identity = 255/573 (44.50%), Postives = 360/573 (62.83%), Query Frame = 1

Query: 7   YGKVDETDQLRLDARRRTRRRIVVIVLSSVVLVAVVVAVVFGTRNASNNNDDSNNQNGSL 66
           YGKVDE   L L  +++TR+R++++ +S VVL+AV++A V  T    N N+ + +    L
Sbjct: 10  YGKVDEAQDLAL--KKKTRKRLLLLSISVVVLIAVIIAAVVATVVHKNKNESTPSPPPEL 69

Query: 67  --STSIKAVCDVTLYPDTCQKAFGPIANSSHLDPGQISKLSVQLALGELSKVADYIFDHA 126
             STS+KA+C VT +P++C  +   + +S+  DP  + KLS+++ + EL  ++D + +  
Sbjct: 70  TPSTSLKAICSVTRFPESCISSISKLPSSNTTDPETLFKLSLKVIIDELDSISD-LPEKL 129

Query: 127 ITKTADNKTILALKNCHELLDLALDHLNISLSSSDITLLKA------VDDLKTWITSAAT 186
             +T D +   AL+ C +L++ ALD LN ++S+ D    K       ++DLKTW+++  T
Sbjct: 130 SKETEDERIKSALRVCGDLIEDALDRLNDTVSAIDDEEKKKTLSSSKIEDLKTWLSATVT 189

Query: 187 YQQTCIDDLAEV--------DPALADLVANFLKNSTELTSNGLAIVSFFSKLTDSLSL-- 246
             +TC D L E+        +  +   + + +  STE TSN LAIVS        L +  
Sbjct: 190 DHETCFDSLDELKQNKTEYANSTITQNLKSAMSRSTEFTSNSLAIVSKILSALSDLGIPI 249

Query: 247 ---RRLMSYENHQSNGDWARPILRKLAQSDIRKHADIVVAKDRSGKYKTITEALGAVPDK 306
              RRLMS+ +HQ + D+ +   R+L Q+   K  D+ VA D +G   T+ EA+  VP K
Sbjct: 250 HRRRRLMSH-HHQQSVDFEKWARRRLLQTAGLK-PDVTVAGDGTGDVLTVNEAVAKVPKK 309

Query: 307 SKKRTVIYVKKGIYKENVEVVKNKWNVVMVGDGMTATIVSGNLNVVDGTPTFSTATFAAK 366
           S K  VIYVK G Y ENV + K+KWNV++ GDG   TI+SG+ N VDGTPT+ TATFA +
Sbjct: 310 SLKMFVIYVKSGTYVENVVMDKSKWNVMIYGDGKGKTIISGSKNFVDGTPTYETATFAIQ 369

Query: 367 GKGFIAIDMGFINTAGPSKHQAVALMSTSDQSIFYRCEMNAYQDTLYAHSNRQFYRECKV 426
           GKGFI  D+G INTAG +KHQAVA  S SD S++Y+C  + +QDTLY HSNRQFYR+C V
Sbjct: 370 GKGFIMKDIGIINTAGAAKHQAVAFRSGSDFSVYYQCSFDGFQDTLYPHSNRQFYRDCDV 429

Query: 427 YGTVDFIFGNSAVVLQNCTIAPRLPLLGQKNTITAQGRFDPNQNTGISIQGSLIQPFNDL 486
            GT+DFIFG++AVV Q C I PR PL  Q NTITAQG+ DPNQ++G+SIQ   I   N  
Sbjct: 430 TGTIDFIFGSAAVVFQGCKIMPRQPLSNQFNTITAQGKKDPNQSSGMSIQRCTISA-NGN 489

Query: 487 KTTETYLGRPWKNYSTTVFMQSNLGRLIHPTGWLPWI-GTSAPDTIFYAEFQNYGPGSST 546
               TYLGRPWK +STTV M++ +G ++ P+GW+ W+ G   P +I Y E++N GPGS  
Sbjct: 490 VIAPTYLGRPWKEFSTTVIMETVIGAVVRPSGWMSWVSGVDPPASIVYGEYKNTGPGSDV 549

Query: 547 SKRVKWKGVKNI-DTKTAKKFTVSSFIDGKDWI 557
           ++RVKW G K +     A KFTV++ + G DWI
Sbjct: 550 TQRVKWAGYKPVMSDAEAAKFTVATLLHGADWI 576

BLAST of Csa1G538170 vs. TAIR10
Match: AT3G14300.1 (AT3G14300.1 pectinesterase family protein)

HSP 1 Score: 404.1 bits (1037), Expect = 1.5e-112
Identity = 237/513 (46.20%), Postives = 307/513 (59.84%), Query Frame = 1

Query: 67  STSIKAVCDVTLYPDTCQKAFGPIANS-SHLDPGQISKLSVQLALGELSKVADYIFDHAI 126
           S+ ++ VC+VT YP +C  +   +  S +  DP  + +LS+Q+   EL+ +   +     
Sbjct: 456 SSVLRTVCNVTNYPASCISSISKLPLSKTTTDPKVLFRLSLQVTFDELNSIVG-LPKKLA 515

Query: 127 TKTADNKTILALKNCHELLDLALDHLNISLSSSDITLL--------KAVDDLKTWITSAA 186
            +T D     AL  C ++ DLA+D +N ++SS D  +           + DL TW++SA 
Sbjct: 516 EETNDEGLKSALSVCADVFDLAVDSVNDTISSLDEVISGGKKNLNSSTIGDLITWLSSAV 575

Query: 187 TYQQTCIDDLAE--VDPALADLVANFLKNSTELTSNGLAIVSFFSKLTDSLSL----RRL 246
           T   TC D L E   +  +   + + + NSTE TSN LAIV+   K      +    RRL
Sbjct: 576 TDIGTCGDTLDEDNYNSPIPQKLKSAMVNSTEFTSNSLAIVAQVLKKPSKSRIPVQGRRL 635

Query: 247 MSYENHQSNGDWARPILRKLAQS-DIRKHADIVVAKDRSGKYKTITEALGAVPDKSKKRT 306
           +   N  S  +W RP +R+L Q+ ++  H  + VA D SG  +T+ EA+  VP K K   
Sbjct: 636 L---NSNSFPNWVRPGVRRLLQAKNLTPH--VTVAADGSGDVRTVNEAVWRVPKKGKTMF 695

Query: 307 VIYVKKGIYKENVEVVKNKWNVVMVGDGMTATIVSGNLNVVDGTPTFSTATFAAKGKGFI 366
           VIYVK G Y ENV + K+KWNV + GDG   TI+SG+ N+VDG  TF+T+TFA +GKGF+
Sbjct: 696 VIYVKAGTYVENVLMKKDKWNVFIYGDGRDKTIISGSTNMVDGVRTFNTSTFATEGKGFM 755

Query: 367 AIDMGFINTAGPSKHQAVALMSTSDQSIFYRCEMNAYQDTLYAHSNRQFYRECKVYGTVD 426
             DMG INTAGP KHQAVA  S SD+S++YRC  + YQDTLY HSNRQ+YR C V GTVD
Sbjct: 756 MKDMGIINTAGPEKHQAVAFRSDSDRSVYYRCSFDGYQDTLYTHSNRQYYRNCDVTGTVD 815

Query: 427 FIFGNSAVVLQNCTIAPRLPLLGQKNTITAQGRFDPNQNTGISIQGSLIQPFNDLKTTET 486
           FIFG   VV Q C+I PR PL  Q NTITA+G  + NQNTGISI    I P N   T  T
Sbjct: 816 FIFGAGTVVFQGCSIRPRQPLPNQFNTITAEGTQEANQNTGISIHQCTISP-NGNVTATT 875

Query: 487 YLGRPWKNYSTTVFMQSNLGRLIHPTGWLPWIGT--SAPDTIFYAEFQNYGPGSSTSKRV 546
           YLGRPWK +S TV MQS +G  ++P GW+ W  T    P TIFY E++N GPGS  SKRV
Sbjct: 876 YLGRPWKLFSKTVIMQSVIGSFVNPAGWIAWNSTYDPPPRTIFYREYKNSGPGSDLSKRV 935

Query: 547 KWKGVKNIDT-KTAKKFTVSSFIDGKD-WISKA 560
           KW G K I +   A +FTV  F+ G D WI KA
Sbjct: 936 KWAGYKPISSDDEAARFTVKYFLRGDDNWIPKA 961

HSP 2 Score: 121.7 bits (304), Expect = 1.5e-27
Identity = 86/254 (33.86%), Postives = 140/254 (55.12%), Query Frame = 1

Query: 2   SFLRPYGKVDETDQLRLDARRRTRRRIVVIVLSSVVLVAVVVA-----VVFGTRNASNNN 61
           S  + YGKVDET  L L  +R+TR+R+  I +S  VLVA++++      +   +  S + 
Sbjct: 6   SINKGYGKVDETQDLAL--KRKTRKRLYQIGISVAVLVAIIISSTVTIAIHSRKGNSPHP 65

Query: 62  DDSNNQNGSLSTSIKAVCDVTLYPDTCQKAFGPIANSSHLDPGQISKLSVQLALGELSKV 121
             S+    + + S+K VC VT YP +C  +   +  S+  DP  I +LS+Q+ + EL+ +
Sbjct: 66  TPSSVPELTPAASLKTVCSVTNYPVSCFSSISKLPLSNTTDPEVIFRLSLQVVIDELNSI 125

Query: 122 ADYIFDHAITKTADNKTILALKNCHELLDLALDHLNISLSSSDIT----LLKA--VDDLK 181
            + +      +T D     AL  C  LLDLA+D +N ++S+ ++     +L A  +DDL 
Sbjct: 126 VE-LPKKLAEETDDEGLKSALSVCEHLLDLAIDRVNETVSAMEVVDGKKILNAATIDDLL 185

Query: 182 TWITSAATYQQTCIDDLAEV---DPALADLVANFLKNSTELTSNGLAIVS-FFSKLTD-- 238
           TW+++A TY  TC+D L E+   + A+   + + + NSTE TSN LAIV+   S ++D  
Sbjct: 186 TWLSAAVTYHGTCLDALDEISHTNSAIPLKLKSGMVNSTEFTSNSLAIVAKILSTISDFG 245

HSP 3 Score: 75.1 bits (183), Expect = 1.6e-13
Identity = 45/168 (26.79%), Postives = 89/168 (52.98%), Query Frame = 1

Query: 67  STSIKAVCDVTLYPDTCQKAFGPIANSSHLDPGQISKLSVQLALGELSKVADYIFDHAIT 126
           + S++ VC VT YP +C  +   + +S+  DP  + +LS+Q+ + EL+ +A  +      
Sbjct: 268 AASLRNVCSVTRYPASCVSSISKLPSSNTTDPEALFRLSLQVVINELNSIAG-LPKKLAE 327

Query: 127 KTADNKTILALKNCHELLDLALDHLNISLSSSD-------ITLLKAVDDLKTWITSAATY 186
           +T D +   +L  C ++ + A+D +N ++S+ +       I     +D+++TW+++A T 
Sbjct: 328 ETDDERLKSSLSVCGDVFNDAIDIVNDTISTMEEVGDGKKILKSSTIDEIQTWLSAAVTD 387

Query: 187 QQTCIDDLAEV--------DPALADLVANFLKNSTELTSNGLAIVSFF 220
             TC+D L E+        +  ++  + + + NS + TSN LAI++ F
Sbjct: 388 HDTCLDALDELSQNKTEYANSPISLKLKSAMVNSRKFTSNSLAIIAKF 434

BLAST of Csa1G538170 vs. TAIR10
Match: AT3G05610.1 (AT3G05610.1 Plant invertase/pectin methylesterase inhibitor superfamily)

HSP 1 Score: 394.8 bits (1013), Expect = 8.9e-110
Identity = 229/571 (40.11%), Postives = 329/571 (57.62%), Query Frame = 1

Query: 19  DARRRTRRRIVVIVLSSVVLVAVVVAVVFGTRNASNNNDDSNNQNGSLSTSIKAVCDV-- 78
           D   + +RR +VI +SSV+L+++VVAV  G   + N +D  +     ++ S+KAV DV  
Sbjct: 6   DDESKRKRRYIVITISSVLLISMVVAVTVGV--SLNKHDGDSKGKAEVNASVKAVKDVCA 65

Query: 79  -TLYPDTCQKAFGPIANSSHL-DPGQISKLSVQLALGELSKVADYIFDHAITKTADNKTI 138
            T Y  TC+     I N  +  DP ++ K +  + + +++  A       +    D++T 
Sbjct: 66  PTDYRKTCEDTL--IKNGKNTTDPMELVKTAFNVTMKQITDAAKKS-QTIMELQKDSRTR 125

Query: 139 LALKNCHELLDLALDHLNIS---LSSSDITLL-KAVDDLKTWITSAATYQQTCIDDLAEV 198
           +AL  C EL+D ALD L+ S   L   +  LL +A+ +L+ W+++A ++++TC++     
Sbjct: 126 MALDQCKELMDYALDELSNSFEELGKFEFHLLDEALINLRIWLSAAISHEETCLEGFQGT 185

Query: 199 DPALADLVANFLKNSTELTSNGLAIVSFFSKLTDSLSL-----RRLMSYENHQSNGDWAR 258
                + +   LK + ELT NGLAI+S  S     + +     RRL++    +    W  
Sbjct: 186 QGNAGETMKKALKTAIELTHNGLAIISEMSNFVGQMQIPGLNSRRLLA----EGFPSWVD 245

Query: 259 PILRKLAQ-----SDIRKHADIVVAKDRSGKYKTITEALGAVPDKSKKRTVIYVKKGIYK 318
              RKL Q     SD++   DIVVA+D SG+YKTI EAL  VP K     V+++K G+YK
Sbjct: 246 QRGRKLLQAAAAYSDVKP--DIVVAQDGSGQYKTINEALQFVPKKRNTTFVVHIKAGLYK 305

Query: 319 ENVEVVKNKWNVVMVGDGMTATIVSGNLNVVDGTPTFSTATFAAKGKGFIAIDMGFINTA 378
           E V+V K   ++V +GDG   TI+SGN N  DG  T+ TAT A  G  FIA ++GF NTA
Sbjct: 306 EYVQVNKTMSHLVFIGDGPDKTIISGNKNYKDGITTYRTATVAIVGNYFIAKNIGFENTA 365

Query: 379 GPSKHQAVALMSTSDQSIFYRCEMNAYQDTLYAHSNRQFYRECKVYGTVDFIFGNSAVVL 438
           G  KHQAVA+   SD+SIF+ C  + YQDTLY HS+RQF+R+C + GT+DF+FG++A V 
Sbjct: 366 GAIKHQAVAVRVQSDESIFFNCRFDGYQDTLYTHSHRQFFRDCTISGTIDFLFGDAAAVF 425

Query: 439 QNCTIAPRLPLLGQKNTITAQGRFDPNQNTGISIQGSLIQPFNDL----KTTETYLGRPW 498
           QNCT+  R PL  Q   ITA GR DP ++TG   QG  I    D     +T++ YLGRPW
Sbjct: 426 QNCTLLVRKPLPNQACPITAHGRKDPRESTGFVFQGCTIAGEPDYLAVKETSKAYLGRPW 485

Query: 499 KNYSTTVFMQSNLGRLIHPTGWLPWIGTSAPDTIFYAEFQNYGPGSSTSKRVKWKGVKNI 558
           K YS T+ M + +   + P GW PW+G     T+FY+E QN GPGS+ + RV W G+K +
Sbjct: 486 KEYSRTIIMNTFIPDFVQPQGWQPWLGDFGLKTLFYSEVQNTGPGSALANRVTWAGIKTL 545

Query: 559 DTKTAKKFTVSSFIDGKDWISKAQVPFTATL 568
             +   KFT + +I G DWI    VP+T  L
Sbjct: 546 SEEDILKFTPAQYIQGDDWIPGKGVPYTTGL 565

BLAST of Csa1G538170 vs. NCBI nr
Match: gi|449435528|ref|XP_004135547.1| (PREDICTED: putative pectinesterase/pectinesterase inhibitor 24 [Cucumis sativus])

HSP 1 Score: 1127.5 bits (2915), Expect = 0.0e+00
Identity = 567/567 (100.00%), Postives = 567/567 (100.00%), Query Frame = 1

Query: 1   MSFLRPYGKVDETDQLRLDARRRTRRRIVVIVLSSVVLVAVVVAVVFGTRNASNNNDDSN 60
           MSFLRPYGKVDETDQLRLDARRRTRRRIVVIVLSSVVLVAVVVAVVFGTRNASNNNDDSN
Sbjct: 1   MSFLRPYGKVDETDQLRLDARRRTRRRIVVIVLSSVVLVAVVVAVVFGTRNASNNNDDSN 60

Query: 61  NQNGSLSTSIKAVCDVTLYPDTCQKAFGPIANSSHLDPGQISKLSVQLALGELSKVADYI 120
           NQNGSLSTSIKAVCDVTLYPDTCQKAFGPIANSSHLDPGQISKLSVQLALGELSKVADYI
Sbjct: 61  NQNGSLSTSIKAVCDVTLYPDTCQKAFGPIANSSHLDPGQISKLSVQLALGELSKVADYI 120

Query: 121 FDHAITKTADNKTILALKNCHELLDLALDHLNISLSSSDITLLKAVDDLKTWITSAATYQ 180
           FDHAITKTADNKTILALKNCHELLDLALDHLNISLSSSDITLLKAVDDLKTWITSAATYQ
Sbjct: 121 FDHAITKTADNKTILALKNCHELLDLALDHLNISLSSSDITLLKAVDDLKTWITSAATYQ 180

Query: 181 QTCIDDLAEVDPALADLVANFLKNSTELTSNGLAIVSFFSKLTDSLSLRRLMSYENHQSN 240
           QTCIDDLAEVDPALADLVANFLKNSTELTSNGLAIVSFFSKLTDSLSLRRLMSYENHQSN
Sbjct: 181 QTCIDDLAEVDPALADLVANFLKNSTELTSNGLAIVSFFSKLTDSLSLRRLMSYENHQSN 240

Query: 241 GDWARPILRKLAQSDIRKHADIVVAKDRSGKYKTITEALGAVPDKSKKRTVIYVKKGIYK 300
           GDWARPILRKLAQSDIRKHADIVVAKDRSGKYKTITEALGAVPDKSKKRTVIYVKKGIYK
Sbjct: 241 GDWARPILRKLAQSDIRKHADIVVAKDRSGKYKTITEALGAVPDKSKKRTVIYVKKGIYK 300

Query: 301 ENVEVVKNKWNVVMVGDGMTATIVSGNLNVVDGTPTFSTATFAAKGKGFIAIDMGFINTA 360
           ENVEVVKNKWNVVMVGDGMTATIVSGNLNVVDGTPTFSTATFAAKGKGFIAIDMGFINTA
Sbjct: 301 ENVEVVKNKWNVVMVGDGMTATIVSGNLNVVDGTPTFSTATFAAKGKGFIAIDMGFINTA 360

Query: 361 GPSKHQAVALMSTSDQSIFYRCEMNAYQDTLYAHSNRQFYRECKVYGTVDFIFGNSAVVL 420
           GPSKHQAVALMSTSDQSIFYRCEMNAYQDTLYAHSNRQFYRECKVYGTVDFIFGNSAVVL
Sbjct: 361 GPSKHQAVALMSTSDQSIFYRCEMNAYQDTLYAHSNRQFYRECKVYGTVDFIFGNSAVVL 420

Query: 421 QNCTIAPRLPLLGQKNTITAQGRFDPNQNTGISIQGSLIQPFNDLKTTETYLGRPWKNYS 480
           QNCTIAPRLPLLGQKNTITAQGRFDPNQNTGISIQGSLIQPFNDLKTTETYLGRPWKNYS
Sbjct: 421 QNCTIAPRLPLLGQKNTITAQGRFDPNQNTGISIQGSLIQPFNDLKTTETYLGRPWKNYS 480

Query: 481 TTVFMQSNLGRLIHPTGWLPWIGTSAPDTIFYAEFQNYGPGSSTSKRVKWKGVKNIDTKT 540
           TTVFMQSNLGRLIHPTGWLPWIGTSAPDTIFYAEFQNYGPGSSTSKRVKWKGVKNIDTKT
Sbjct: 481 TTVFMQSNLGRLIHPTGWLPWIGTSAPDTIFYAEFQNYGPGSSTSKRVKWKGVKNIDTKT 540

Query: 541 AKKFTVSSFIDGKDWISKAQVPFTATL 568
           AKKFTVSSFIDGKDWISKAQVPFTATL
Sbjct: 541 AKKFTVSSFIDGKDWISKAQVPFTATL 567

BLAST of Csa1G538170 vs. NCBI nr
Match: gi|659068622|ref|XP_008445366.1| (PREDICTED: putative pectinesterase/pectinesterase inhibitor 24 [Cucumis melo])

HSP 1 Score: 1068.1 bits (2761), Expect = 5.1e-309
Identity = 536/567 (94.53%), Postives = 550/567 (97.00%), Query Frame = 1

Query: 1   MSFLRPYGKVDETDQLRLDARRRTRRRIVVIVLSSVVLVAVVVAVVFGTRNASNNNDDSN 60
           MSFLRPYGKVDETDQLRLDA RRTRRRIVVIVLSSVVLVAVVVAVVFGTRNASNNNDDSN
Sbjct: 1   MSFLRPYGKVDETDQLRLDASRRTRRRIVVIVLSSVVLVAVVVAVVFGTRNASNNNDDSN 60

Query: 61  NQNGSLSTSIKAVCDVTLYPDTCQKAFGPIANSSHLDPGQISKLSVQLALGELSKVADYI 120
           NQNGSLS SIKAVCDVTLYPDTCQKAFGP ANSSHLDPGQISKL+VQLALGELSKVAD+I
Sbjct: 61  NQNGSLSASIKAVCDVTLYPDTCQKAFGPFANSSHLDPGQISKLAVQLALGELSKVADHI 120

Query: 121 FDHAITKTADNKTILALKNCHELLDLALDHLNISLSSSDITLLKAVDDLKTWITSAATYQ 180
           FDHAIT T D+KTILALKNCHELLDLALDHLNI+LSSSDITLLKAVDDLKTW+TSAATYQ
Sbjct: 121 FDHAITNTTDDKTILALKNCHELLDLALDHLNITLSSSDITLLKAVDDLKTWLTSAATYQ 180

Query: 181 QTCIDDLAEVDPALADLVANFLKNSTELTSNGLAIVSFFSKLTDSLSLRRLMSYENHQSN 240
           QTCID LAEVDPALADL ANFLKNSTELTSNGLAIVSFFSKLTDSLSLRRLMSYE+ QSN
Sbjct: 181 QTCIDGLAEVDPALADLFANFLKNSTELTSNGLAIVSFFSKLTDSLSLRRLMSYEDRQSN 240

Query: 241 GDWARPILRKLAQSDIRKHADIVVAKDRSGKYKTITEALGAVPDKSKKRTVIYVKKGIYK 300
           GDWARPILRKLAQSDIRK ADIVVAKD SGKYKTITEAL AVPDKSKKRTVIYVKKG YK
Sbjct: 241 GDWARPILRKLAQSDIRKQADIVVAKDSSGKYKTITEALKAVPDKSKKRTVIYVKKGTYK 300

Query: 301 ENVEVVKNKWNVVMVGDGMTATIVSGNLNVVDGTPTFSTATFAAKGKGFIAIDMGFINTA 360
           E VEVVKNKWNVVMVGDGM ATIVSG+LNV+DGTPTFSTA+FAAKGKGFIAIDMGFINTA
Sbjct: 301 EKVEVVKNKWNVVMVGDGMNATIVSGSLNVIDGTPTFSTASFAAKGKGFIAIDMGFINTA 360

Query: 361 GPSKHQAVALMSTSDQSIFYRCEMNAYQDTLYAHSNRQFYRECKVYGTVDFIFGNSAVVL 420
           GPSKHQAVALMSTSDQSIFYRC+MNAYQDTLYAHSNRQFYRECKVYGTVDFIFGNSAVVL
Sbjct: 361 GPSKHQAVALMSTSDQSIFYRCQMNAYQDTLYAHSNRQFYRECKVYGTVDFIFGNSAVVL 420

Query: 421 QNCTIAPRLPLLGQKNTITAQGRFDPNQNTGISIQGSLIQPFNDLKTTETYLGRPWKNYS 480
           QNCTIAPRLPLLGQKNTITAQGRFDPNQNTGISIQGSLIQPF++LKTTETYLGRPWKNYS
Sbjct: 421 QNCTIAPRLPLLGQKNTITAQGRFDPNQNTGISIQGSLIQPFDNLKTTETYLGRPWKNYS 480

Query: 481 TTVFMQSNLGRLIHPTGWLPWIGTSAPDTIFYAEFQNYGPGSSTSKRVKWKGVKNIDTKT 540
           TTVFMQSNLG LIHP+GWLPW+GTSAPDTIFYAEFQNYG GSSTSKRVKWKGVKNID KT
Sbjct: 481 TTVFMQSNLGSLIHPSGWLPWVGTSAPDTIFYAEFQNYGRGSSTSKRVKWKGVKNIDIKT 540

Query: 541 AKKFTVSSFIDGKDWISKAQVPFTATL 568
           AKKFTVSSFIDGKDWISKAQVPFTATL
Sbjct: 541 AKKFTVSSFIDGKDWISKAQVPFTATL 567

BLAST of Csa1G538170 vs. NCBI nr
Match: gi|694422862|ref|XP_009339254.1| (PREDICTED: putative pectinesterase/pectinesterase inhibitor 24 [Pyrus x bretschneideri])

HSP 1 Score: 707.6 bits (1825), Expect = 1.8e-200
Identity = 357/575 (62.09%), Postives = 445/575 (77.39%), Query Frame = 1

Query: 1   MSFLRPYGKVDETDQLRLDARRRTRRRIVVIVLSSVVLVAVVVAVVFGTRNASNNNDDSN 60
           MS  + YGKVDE +Q RLDARR+TR+R+ +I LSS+VLV +VVA V GT    +N    N
Sbjct: 1   MSSFKSYGKVDEYEQARLDARRKTRKRVAIISLSSIVLVCIVVAAVVGT--TVHNKSGGN 60

Query: 61  NQNGSLSTSIKAVCDVTLYPDTCQKAFGPIANSSHLDPGQISKLSVQLALGELSKVADYI 120
           ++   +STS+KAVCDVTLY D+C  + GP+ NS+++ P ++ KL+VQ+A  EL+K A + 
Sbjct: 61  SEGSGISTSVKAVCDVTLYQDSCYSSLGPLVNSNNVKPEELFKLAVQVANNELTKAASHF 120

Query: 121 F-DHAITKTA-DNKTILALKNCHELLDLALDHLNISLSSSDITLLKAVDDLKTWITSAAT 180
             D  +  TA D K+  ALK+C +LL LALDHLN +LSS++ +L+   DDLKTW++S+ T
Sbjct: 121 SADGVLNATAKDRKSAAALKDCQDLLSLALDHLNDTLSSAN-SLVDVFDDLKTWLSSSGT 180

Query: 181 YQQTCIDDLAEVDPALADLVANFLKNSTELTSNGLAIVSFFSKLTDSLSLRRLMSYEN-- 240
           YQQTCI+   EV   L D V  +LKNSTELTSN LAIVS+ SK+ +S+ LRRL+++ +  
Sbjct: 181 YQQTCIEGFDEVKGELKDKVIGYLKNSTELTSNSLAIVSWISKILNSVKLRRLLTHPDPT 240

Query: 241 ---HQSNGDWARPILRKLAQS-DIRKHADIVVAKDRSGKYKTITEALGAVPDKSKKRTVI 300
               Q   +W     RKL QS D+RK ADIVVA+D SGKYKTI +AL AVP+KSKKRT+I
Sbjct: 241 DSYSQGVPEWLHRKDRKLLQSSDLRKKADIVVARDGSGKYKTIHDALKAVPNKSKKRTII 300

Query: 301 YVKKGIYKENVEVVKNKWNVVMVGDGMTATIVSGNLNVVDGTPTFSTATFAAKGKGFIAI 360
           YVKKG+Y  NV V K+KWNV++VGDG TATIVS  LNVVDGTPTFSTATFA  G+GFIA 
Sbjct: 301 YVKKGVYYGNVRVEKSKWNVLVVGDGSTATIVSAKLNVVDGTPTFSTATFAVFGRGFIAR 360

Query: 361 DMGFINTAGPSKHQAVALMSTSDQSIFYRCEMNAYQDTLYAHSNRQFYRECKVYGTVDFI 420
           D+GF NTAGP+KHQAVALMST+DQS+FYRC ++A+QDTLYAHSNRQFYREC +YGTVDFI
Sbjct: 361 DIGFRNTAGPAKHQAVALMSTADQSVFYRCHIDAFQDTLYAHSNRQFYRECNIYGTVDFI 420

Query: 421 FGNSAVVLQNCTIAPRLPLLGQKNTITAQGRFDPNQNTGISIQGSLIQPFNDLKTTETYL 480
           FGNSAVVLQNC I P++P+ GQ+NTITAQG+ DPNQNTGISI    I PF DL   +TYL
Sbjct: 421 FGNSAVVLQNCNIMPKVPMPGQQNTITAQGKIDPNQNTGISIHNCSISPFGDLSRVKTYL 480

Query: 481 GRPWKNYSTTVFMQSNLGRLIHPTGWLPWIGTSAPDTIFYAEFQNYGPGSSTSKRVKWKG 540
           GRPWKN+STTV+MQ+ +G LI+P GW+PW+GTSAPDTIFY+EFQN+GPGSST  RVKWKG
Sbjct: 481 GRPWKNHSTTVYMQTYMGSLINPNGWMPWVGTSAPDTIFYSEFQNFGPGSSTKNRVKWKG 540

Query: 541 VKNIDTKTAKKFTVSSFIDGKDWISKAQVPFTATL 568
           +++I+ K A KFTV SF+ G  WIS A V + + L
Sbjct: 541 LRSINQKAAGKFTVHSFLQGTKWISDAGVTYKSNL 572

BLAST of Csa1G538170 vs. NCBI nr
Match: gi|645251887|ref|XP_008231878.1| (PREDICTED: probable pectinesterase/pectinesterase inhibitor 46 [Prunus mume])

HSP 1 Score: 699.1 bits (1803), Expect = 6.2e-198
Identity = 358/577 (62.05%), Postives = 434/577 (75.22%), Query Frame = 1

Query: 1   MSFLRPYGKVDETDQLRLDARRRTRRRIVVIVLSSVVLVAVVVAVVFGTRNASNNNDDSN 60
           MS  + YGKV+E +Q RLDARR+TRRR+V+I LSS+VLV VVVA V GT    NN+    
Sbjct: 1   MSSFKAYGKVNELEQSRLDARRKTRRRVVIISLSSIVLVCVVVAAVVGTTIHKNNS--GG 60

Query: 61  NQNGSLSTSIKAVCDVTLYPDTCQKAFGPIANSSHLDPGQISKLSVQLALGELSKVADYI 120
            Q  SLSTS+KAVCDVTLY D+C  + G + NS++  P  + KL++++A+ ELSK A   
Sbjct: 61  KQGSSLSTSVKAVCDVTLYQDSCYSSLGALVNSTNAKPEDLFKLAIRVAINELSKAASRF 120

Query: 121 FDHAITKTADNKTI--LALKNCHELLDLALDHLNISLSSSDITLLKAVDDLKTWITSAAT 180
               I     N T+   AL+NC ELL LALDHLN SLS  D +L    DDLKTW++S+ T
Sbjct: 121 SGDRILNGTSNDTLSKAALENCQELLRLALDHLNDSLSD-DKSLFDVFDDLKTWLSSSGT 180

Query: 181 YQQTCIDDLAEVDPALADLVANFLKNSTELTSNGLAIVSFFSKLTDSLSLRRLMSYEN-- 240
           YQQTCID   +    L D +A++LKNSTELTSN LAIVS+ SK+  S+ LRRL+++    
Sbjct: 181 YQQTCIDGFDDAKAELKDKIASYLKNSTELTSNSLAIVSWISKVVSSVKLRRLLTHPATT 240

Query: 241 ---HQSNG--DWARPILRKLAQS-DIRKHADIVVAKDRSGKYKTITEALGAVPDKSKKRT 300
              + S G  +W  P  RKL +S D+RK ADIVVA D SGKYKTI  AL  VPDKSKKRT
Sbjct: 241 ATANYSEGVPEWLHPRDRKLLESSDLRKKADIVVASDGSGKYKTIHAALKVVPDKSKKRT 300

Query: 301 VIYVKKGIYKENVEVVKNKWNVVMVGDGMTATIVSGNLNVVDGTPTFSTATFAAKGKGFI 360
           VIYVKKG+Y ENV V K KWNV++VGDG TATIVS   NV+DGTPTFS+ATFA  GKGFI
Sbjct: 301 VIYVKKGVYFENVRVEKTKWNVLVVGDGSTATIVSAGRNVIDGTPTFSSATFAVFGKGFI 360

Query: 361 AIDMGFINTAGPSKHQAVALMSTSDQSIFYRCEMNAYQDTLYAHSNRQFYRECKVYGTVD 420
           A D+GF NTAG +KHQAVALMST+DQS+FYRC ++A+QDTLYAHSNRQFYREC +YGTVD
Sbjct: 361 ARDIGFRNTAGAAKHQAVALMSTADQSVFYRCHIDAFQDTLYAHSNRQFYRECNIYGTVD 420

Query: 421 FIFGNSAVVLQNCTIAPRLPLLGQKNTITAQGRFDPNQNTGISIQGSLIQPFNDLKTTET 480
           FIFGNSAVVLQNC I P+ P+ GQ+NTITAQG+ DPNQNTGISIQ   I P+ DL + +T
Sbjct: 421 FIFGNSAVVLQNCNIMPKRPMAGQQNTITAQGKIDPNQNTGISIQNCSISPYGDLSSVQT 480

Query: 481 YLGRPWKNYSTTVFMQSNLGRLIHPTGWLPWIGTSAPDTIFYAEFQNYGPGSSTSKRVKW 540
           YLGRPWKNYSTTV+MQ+ +G LI+P+GWLPW+G +APDTIFY+EFQN+GPGSST  RVKW
Sbjct: 481 YLGRPWKNYSTTVYMQTFMGSLINPSGWLPWVGNTAPDTIFYSEFQNFGPGSSTRNRVKW 540

Query: 541 KGVKNIDTKTAKKFTVSSFIDGKDWISKAQVPFTATL 568
           KG++ I++K A KFTV S + G  WIS A V + + L
Sbjct: 541 KGLRTINSKEAGKFTVHSLLQGNKWISDAGVTYKSNL 574

BLAST of Csa1G538170 vs. NCBI nr
Match: gi|694328524|ref|XP_009355064.1| (PREDICTED: putative pectinesterase/pectinesterase inhibitor 24 [Pyrus x bretschneideri])

HSP 1 Score: 698.7 bits (1802), Expect = 8.2e-198
Identity = 350/575 (60.87%), Postives = 439/575 (76.35%), Query Frame = 1

Query: 1   MSFLRPYGKVDETDQLRLDARRRTRRRIVVIVLSSVVLVAVVVAVVFGTRNASNNNDDSN 60
           MS ++ YGK+DE++Q RLD RR+TR R+ +I LSS+VLV VVVA V GT    +N    N
Sbjct: 1   MSSIKSYGKIDESEQARLDNRRKTRMRVAIISLSSIVLVCVVVAAVVGT--TVHNKSGGN 60

Query: 61  NQNGSLSTSIKAVCDVTLYPDTCQKAFGPIANSSHLDPGQISKLSVQLALGELSKVADYI 120
           ++  ++STS+KAVCDVTLY D C  + GP+ NSS++ P +I KL++Q+A+ ELSK A + 
Sbjct: 61  SKGSAISTSVKAVCDVTLYQDLCYSSLGPLVNSSNVKPEEIFKLAIQVAINELSKAATHF 120

Query: 121 FDHAITKTA--DNKTILALKNCHELLDLALDHLNISLSSSDITLLKAVDDLKTWITSAAT 180
               +      D +++ ALK+C +LL LALDHLN SLS +  +L+   DDLKTW++S+ T
Sbjct: 121 SADGVLNAIAKDTESVAALKDCQDLLSLALDHLNDSLSDAK-SLVDVFDDLKTWLSSSGT 180

Query: 181 YQQTCIDDLAEVDPALADLVANFLKNSTELTSNGLAIVSFFSKLTDSLSLRRLMSYEN-- 240
           YQQTCI+   +V   L D V  +LKNS ELTSN LAIVS+ SK+ +S++LRRL+++ +  
Sbjct: 181 YQQTCIEGFDDVKGELKDKVVGYLKNSNELTSNSLAIVSWISKILNSVNLRRLLTHPDPT 240

Query: 241 ---HQSNGDWARPILRKLAQS-DIRKHADIVVAKDRSGKYKTITEALGAVPDKSKKRTVI 300
               Q   +W     RKL QS D+RK ADIVVA + S KYKTI EAL AVPDKSKKRTVI
Sbjct: 241 DKYSQGVPEWLDRKDRKLLQSSDLRKKADIVVASNGSSKYKTIQEALKAVPDKSKKRTVI 300

Query: 301 YVKKGIYKENVEVVKNKWNVVMVGDGMTATIVSGNLNVVDGTPTFSTATFAAKGKGFIAI 360
           YVKKG+Y  NV V K+KWNV++VGDG  ATIV+  LNVVDGTPTFSTATFA  GKGFIA 
Sbjct: 301 YVKKGVYYGNVRVEKSKWNVLVVGDGSNATIVTAKLNVVDGTPTFSTATFAVFGKGFIAR 360

Query: 361 DMGFINTAGPSKHQAVALMSTSDQSIFYRCEMNAYQDTLYAHSNRQFYRECKVYGTVDFI 420
           D+GF NTAGP+KHQAVAL+ST+DQS+FYRC+++A+QDTLYAHSNRQFYREC +YGTVDFI
Sbjct: 361 DIGFRNTAGPAKHQAVALLSTADQSVFYRCDIDAFQDTLYAHSNRQFYRECNIYGTVDFI 420

Query: 421 FGNSAVVLQNCTIAPRLPLLGQKNTITAQGRFDPNQNTGISIQGSLIQPFNDLKTTETYL 480
           FGNSAVVLQNC I PR+P+ GQ+NTITAQG+FDPNQNTGISI    I P  DL + +T+L
Sbjct: 421 FGNSAVVLQNCNIMPRVPMPGQQNTITAQGKFDPNQNTGISIHNCSISPLGDLSSVKTFL 480

Query: 481 GRPWKNYSTTVFMQSNLGRLIHPTGWLPWIGTSAPDTIFYAEFQNYGPGSSTSKRVKWKG 540
           GRPWKNYSTTV+MQ+ +G LI   GWLPW+G SAPDT+FY+EFQN+GPG+ST  RVKWKG
Sbjct: 481 GRPWKNYSTTVYMQTYMGNLISTDGWLPWVGNSAPDTVFYSEFQNFGPGASTKNRVKWKG 540

Query: 541 VKNIDTKTAKKFTVSSFIDGKDWISKAQVPFTATL 568
           ++ I+ K A KFTV SF+ G  WIS A V +T++L
Sbjct: 541 LRTINQKAASKFTVHSFLQGTKWISDAGVTYTSSL 572