CsaV3_1G037280 (gene) Cucumber (Chinese Long) v3

BLAST of CsaV3_1G037280 vs. NCBI nr
Match: XP_004135547.1 (PREDICTED: putative pectinesterase/pectinesterase inhibitor 24 [Cucumis sativus] >KGN65919.1 hypothetical protein Csa_1G538170 [Cucumis sativus])

HSP 1 Score: 1026.5 bits (2653), Expect = 3.3e-296
Identity = 567/567 (100.00%), Postives = 567/567 (100.00%), Query Frame = 0

Query: 1   MSFLRPYGKVDETDQLRLDARXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 60
           MSFLRPYGKVDETDQLRLDARXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX
Sbjct: 1   MSFLRPYGKVDETDQLRLDARXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 60

Query: 61  XXXXSLSTSIKAVCDVTLYPDTCQKAFGPIANSSHLDPGQISKLSVQLALGELSKVADYI 120
           XXXXSLSTSIKAVCDVTLYPDTCQKAFGPIANSSHLDPGQISKLSVQLALGELSKVADYI
Sbjct: 61  XXXXSLSTSIKAVCDVTLYPDTCQKAFGPIANSSHLDPGQISKLSVQLALGELSKVADYI 120

Query: 121 FDHAITKTADNKTILALKNCHELLDLALDHLNISLSSSDITLLKAVDDLKTWITSAATYQ 180
           FDHAITKTADNKTILALKNCHELLDLALDHLNISLSSSDITLLKAVDDLKTWITSAATYQ
Sbjct: 121 FDHAITKTADNKTILALKNCHELLDLALDHLNISLSSSDITLLKAVDDLKTWITSAATYQ 180

Query: 181 QTCIDDLAEVDPALADLVANFLKNSTELTSNGLAIVSFFSKLTDSLSLRRLMSYENHQSN 240
           QTCIDDLAEVDPALADLVANFLKNSTELTSNGLAIVSFFSKLTDSLSLRRLMSYENHQSN
Sbjct: 181 QTCIDDLAEVDPALADLVANFLKNSTELTSNGLAIVSFFSKLTDSLSLRRLMSYENHQSN 240

Query: 241 GDWARPILRKLAQSDIRKHADIVVAKDRSGKYKTITEALGAVPDKSKKRTVIYVKKGIYK 300
           GDWARPILRKLAQSDIRKHADIVVAKDRSGKYKTITEALGAVPDKSKKRTVIYVKKGIYK
Sbjct: 241 GDWARPILRKLAQSDIRKHADIVVAKDRSGKYKTITEALGAVPDKSKKRTVIYVKKGIYK 300

Query: 301 ENVEVVKNKWNVVMVGDGMTATIVSGNLNVVDGTPTFSTATFAAKGKGFIAIDMGFINTA 360
           ENVEVVKNKWNVVMVGDGMTATIVSGNLNVVDGTPTFSTATFAAKGKGFIAIDMGFINTA
Sbjct: 301 ENVEVVKNKWNVVMVGDGMTATIVSGNLNVVDGTPTFSTATFAAKGKGFIAIDMGFINTA 360

Query: 361 GPSKHQAVALMSTSDQSIFYRCEMNAYQDTLYAHSNRQFYRECKVYGTVDFIFGNSAVVL 420
           GPSKHQAVALMSTSDQSIFYRCEMNAYQDTLYAHSNRQFYRECKVYGTVDFIFGNSAVVL
Sbjct: 361 GPSKHQAVALMSTSDQSIFYRCEMNAYQDTLYAHSNRQFYRECKVYGTVDFIFGNSAVVL 420

Query: 421 QNCTIAPRLPLLGQKNTITAQGRFDPNQNTGISIQGSLIQPFNDLKTTETYLGRPWKNYS 480
           QNCTIAPRLPLLGQKNTITAQGRFDPNQNTGISIQGSLIQPFNDLKTTETYLGRPWKNYS
Sbjct: 421 QNCTIAPRLPLLGQKNTITAQGRFDPNQNTGISIQGSLIQPFNDLKTTETYLGRPWKNYS 480

Query: 481 TTVFMQSNLGRLIHPTGWLPWIGTSAPDTIFYAEFQNYGPGSSTSKRVKWKGVKNIDTKT 540
           TTVFMQSNLGRLIHPTGWLPWIGTSAPDTIFYAEFQNYGPGSSTSKRVKWKGVKNIDTKT
Sbjct: 481 TTVFMQSNLGRLIHPTGWLPWIGTSAPDTIFYAEFQNYGPGSSTSKRVKWKGVKNIDTKT 540

Query: 541 AKKFTVSSFIDGKDWISKAQVPFTATL 568
           AKKFTVSSFIDGKDWISKAQVPFTATL
Sbjct: 541 AKKFTVSSFIDGKDWISKAQVPFTATL 567

BLAST of CsaV3_1G037280 vs. NCBI nr
Match: XP_008445366.1 (PREDICTED: putative pectinesterase/pectinesterase inhibitor 24 [Cucumis melo])

HSP 1 Score: 967.2 bits (2499), Expect = 2.4e-278
Identity = 536/567 (94.53%), Postives = 550/567 (97.00%), Query Frame = 0

Query: 1   MSFLRPYGKVDETDQLRLDARXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 60
           MSFLRPYGKVDETDQLRLDA XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX
Sbjct: 1   MSFLRPYGKVDETDQLRLDASXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 60

Query: 61  XXXXSLSTSIKAVCDVTLYPDTCQKAFGPIANSSHLDPGQISKLSVQLALGELSKVADYI 120
           XXXXSLS SIKAVCDVTLYPDTCQKAFGP ANSSHLDPGQISKL+VQLALGELSKVAD+I
Sbjct: 61  XXXXSLSASIKAVCDVTLYPDTCQKAFGPFANSSHLDPGQISKLAVQLALGELSKVADHI 120

Query: 121 FDHAITKTADNKTILALKNCHELLDLALDHLNISLSSSDITLLKAVDDLKTWITSAATYQ 180
           FDHAIT T D+KTILALKNCHELLDLALDHLNI+LSSSDITLLKAVDDLKTW+TSAATYQ
Sbjct: 121 FDHAITNTTDDKTILALKNCHELLDLALDHLNITLSSSDITLLKAVDDLKTWLTSAATYQ 180

Query: 181 QTCIDDLAEVDPALADLVANFLKNSTELTSNGLAIVSFFSKLTDSLSLRRLMSYENHQSN 240
           QTCID LAEVDPALADL ANFLKNSTELTSNGLAIVSFFSKLTDSLSLRRLMSYE+ QSN
Sbjct: 181 QTCIDGLAEVDPALADLFANFLKNSTELTSNGLAIVSFFSKLTDSLSLRRLMSYEDRQSN 240

Query: 241 GDWARPILRKLAQSDIRKHADIVVAKDRSGKYKTITEALGAVPDKSKKRTVIYVKKGIYK 300
           GDWARPILRKLAQSDIRK ADIVVAKD SGKYKTITEAL AVPDKSKKRTVIYVKKG YK
Sbjct: 241 GDWARPILRKLAQSDIRKQADIVVAKDSSGKYKTITEALKAVPDKSKKRTVIYVKKGTYK 300

Query: 301 ENVEVVKNKWNVVMVGDGMTATIVSGNLNVVDGTPTFSTATFAAKGKGFIAIDMGFINTA 360
           E VEVVKNKWNVVMVGDGM ATIVSG+LNV+DGTPTFSTA+FAAKGKGFIAIDMGFINTA
Sbjct: 301 EKVEVVKNKWNVVMVGDGMNATIVSGSLNVIDGTPTFSTASFAAKGKGFIAIDMGFINTA 360

Query: 361 GPSKHQAVALMSTSDQSIFYRCEMNAYQDTLYAHSNRQFYRECKVYGTVDFIFGNSAVVL 420
           GPSKHQAVALMSTSDQSIFYRC+MNAYQDTLYAHSNRQFYRECKVYGTVDFIFGNSAVVL
Sbjct: 361 GPSKHQAVALMSTSDQSIFYRCQMNAYQDTLYAHSNRQFYRECKVYGTVDFIFGNSAVVL 420

Query: 421 QNCTIAPRLPLLGQKNTITAQGRFDPNQNTGISIQGSLIQPFNDLKTTETYLGRPWKNYS 480
           QNCTIAPRLPLLGQKNTITAQGRFDPNQNTGISIQGSLIQPF++LKTTETYLGRPWKNYS
Sbjct: 421 QNCTIAPRLPLLGQKNTITAQGRFDPNQNTGISIQGSLIQPFDNLKTTETYLGRPWKNYS 480

Query: 481 TTVFMQSNLGRLIHPTGWLPWIGTSAPDTIFYAEFQNYGPGSSTSKRVKWKGVKNIDTKT 540
           TTVFMQSNLG LIHP+GWLPW+GTSAPDTIFYAEFQNYG GSSTSKRVKWKGVKNID KT
Sbjct: 481 TTVFMQSNLGSLIHPSGWLPWVGTSAPDTIFYAEFQNYGRGSSTSKRVKWKGVKNIDIKT 540

Query: 541 AKKFTVSSFIDGKDWISKAQVPFTATL 568
           AKKFTVSSFIDGKDWISKAQVPFTATL
Sbjct: 541 AKKFTVSSFIDGKDWISKAQVPFTATL 567

BLAST of CsaV3_1G037280 vs. NCBI nr
Match: XP_022927225.1 (putative pectinesterase/pectinesterase inhibitor 24 [Cucurbita moschata])

HSP 1 Score: 897.5 bits (2318), Expect = 2.3e-257
Identity = 478/567 (84.30%), Postives = 518/567 (91.36%), Query Frame = 0

Query: 1   MSFLRPYGKVDETDQLRLDARXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 60
           MS LRPYGKVDE+DQ+RLDA      XXXXXXXXXXXXXXXXXXXXXXXXXXXX      
Sbjct: 1   MSLLRPYGKVDESDQMRLDA-SRRTRXXXXXXXXXXXXXXXXXXXXXXXXXXXXKNDESD 60

Query: 61  XXXXSLSTSIKAVCDVTLYPDTCQKAFGPIANSSHLDPGQISKLSVQLALGELSKVADYI 120
               +LSTSIKAVCD TLYPDTC+ AFG +ANSSHLDPGQI+K SVQLALGELS+VADY+
Sbjct: 61  NHNEALSTSIKAVCDETLYPDTCKNAFGSLANSSHLDPGQIAKFSVQLALGELSRVADYL 120

Query: 121 FDHAITKTADNKTILALKNCHELLDLALDHLNISLSSSDITLLKAVDDLKTWITSAATYQ 180
            +HAIT + DNKTILAL++CHELLDLA+DHLN SLSSS+IT+LKAVDDLKTW++SAATYQ
Sbjct: 121 LEHAITNSTDNKTILALRSCHELLDLAIDHLNDSLSSSEITVLKAVDDLKTWLSSAATYQ 180

Query: 181 QTCIDDLAEVDPALADLVANFLKNSTELTSNGLAIVSFFSKLTDSLSLRRLMSYENHQSN 240
           QTCID L EVD AL +++AN+LKNSTE+TSNGLAIVSFFSKLTDS SLRRLMSYE+  SN
Sbjct: 181 QTCIDGLEEVDLALTNVIANYLKNSTEMTSNGLAIVSFFSKLTDSFSLRRLMSYEDRHSN 240

Query: 241 GDWARPILRKLAQSDIRKHADIVVAKDRSGKYKTITEALGAVPDKSKKRTVIYVKKGIYK 300
           GDWARPILRKLAQ D+RK ADIVVAKD+SGKYKTITEAL AVPDKSKKRTVIYVKKG+YK
Sbjct: 241 GDWARPILRKLAQKDLRKQADIVVAKDKSGKYKTITEALNAVPDKSKKRTVIYVKKGVYK 300

Query: 301 ENVEVVKNKWNVVMVGDGMTATIVSGNLNVVDGTPTFSTATFAAKGKGFIAIDMGFINTA 360
           ENVEVVKNKWNVVM+GDGMT+T V+G LN +DGTPTFSTATFAAKGKGFIAIDMGF NTA
Sbjct: 301 ENVEVVKNKWNVVMIGDGMTSTTVTGRLNFIDGTPTFSTATFAAKGKGFIAIDMGFENTA 360

Query: 361 GPSKHQAVALMSTSDQSIFYRCEMNAYQDTLYAHSNRQFYRECKVYGTVDFIFGNSAVVL 420
           GPSKHQAVALMSTSDQSIFYRC+MNAYQDTLYAHSNRQFYR+CKVYGTVDFIFGNSAVVL
Sbjct: 361 GPSKHQAVALMSTSDQSIFYRCQMNAYQDTLYAHSNRQFYRDCKVYGTVDFIFGNSAVVL 420

Query: 421 QNCTIAPRLPLLGQKNTITAQGRFDPNQNTGISIQGSLIQPFNDLKTTETYLGRPWKNYS 480
           QNC IAP+LPLLGQKNTITAQGRFDPNQ+TGISIQ S IQPF +LKTTETYLGRPWKNYS
Sbjct: 421 QNCKIAPKLPLLGQKNTITAQGRFDPNQHTGISIQHSSIQPFENLKTTETYLGRPWKNYS 480

Query: 481 TTVFMQSNLGRLIHPTGWLPWIGTSAPDTIFYAEFQNYGPGSSTSKRVKWKGVKNIDTKT 540
           TTVFMQ++ G LIHPTGWLPW+GTSAPDTIFYAEFQN GPGSST+ RVKWKG+KNIDTKT
Sbjct: 481 TTVFMQNDFGSLIHPTGWLPWVGTSAPDTIFYAEFQNKGPGSSTANRVKWKGLKNIDTKT 540

Query: 541 AKKFTVSSFIDGKDWISKAQVPFTATL 568
           AKKFTVSSFI GKDWISKAQVPF ATL
Sbjct: 541 AKKFTVSSFIHGKDWISKAQVPFNATL 566

BLAST of CsaV3_1G037280 vs. NCBI nr
Match: XP_023000829.1 (probable pectinesterase/pectinesterase inhibitor 46 [Cucurbita maxima])

HSP 1 Score: 896.7 bits (2316), Expect = 4.0e-257
Identity = 478/567 (84.30%), Postives = 516/567 (91.01%), Query Frame = 0

Query: 1   MSFLRPYGKVDETDQLRLDARXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 60
           MS LRPYGKVDE+DQ+RLDA      XXXXXXXXXXXXXXXXXXXXXXXXXXXX      
Sbjct: 1   MSLLRPYGKVDESDQMRLDA-SRRTRXXXXXXXXXXXXXXXXXXXXXXXXXXXXKNDESD 60

Query: 61  XXXXSLSTSIKAVCDVTLYPDTCQKAFGPIANSSHLDPGQISKLSVQLALGELSKVADYI 120
               +LSTSIKAVCD TLYPDTC  AFGP+ANSSHLDPGQI+K SVQLAL ELS+VADY+
Sbjct: 61  NHNEALSTSIKAVCDETLYPDTCNNAFGPLANSSHLDPGQIAKFSVQLALSELSRVADYL 120

Query: 121 FDHAITKTADNKTILALKNCHELLDLALDHLNISLSSSDITLLKAVDDLKTWITSAATYQ 180
            +HAIT + DNKTILAL+ CHELLDLA+DHLN SLSSS+IT+LKAVDDLKTW++SAATYQ
Sbjct: 121 LEHAITNSTDNKTILALRGCHELLDLAMDHLNDSLSSSEITMLKAVDDLKTWLSSAATYQ 180

Query: 181 QTCIDDLAEVDPALADLVANFLKNSTELTSNGLAIVSFFSKLTDSLSLRRLMSYENHQSN 240
           QTCID L EVD AL +++AN+LKNSTE+TSNGLAIVSFFSKLTDS SLRRLMSYE+  SN
Sbjct: 181 QTCIDGLEEVDLALMNVIANYLKNSTEMTSNGLAIVSFFSKLTDSFSLRRLMSYEDRHSN 240

Query: 241 GDWARPILRKLAQSDIRKHADIVVAKDRSGKYKTITEALGAVPDKSKKRTVIYVKKGIYK 300
           GDWARPILRKLAQ D+RK ADIVVAKD+SGKYKTITEAL AV DKSKKRTVIYVKKG+YK
Sbjct: 241 GDWARPILRKLAQKDLRKQADIVVAKDKSGKYKTITEALKAVRDKSKKRTVIYVKKGVYK 300

Query: 301 ENVEVVKNKWNVVMVGDGMTATIVSGNLNVVDGTPTFSTATFAAKGKGFIAIDMGFINTA 360
           ENVEVVKNKWNVVM+GDGMT+T V+G LN +DGTPTFSTATFAAKGKGFIAIDMGF NTA
Sbjct: 301 ENVEVVKNKWNVVMIGDGMTSTTVTGKLNFIDGTPTFSTATFAAKGKGFIAIDMGFENTA 360

Query: 361 GPSKHQAVALMSTSDQSIFYRCEMNAYQDTLYAHSNRQFYRECKVYGTVDFIFGNSAVVL 420
           GPSKHQAVALMSTSDQSIFYRC+MNAYQDTLYAHSNRQFYR+CKVYGTVDFIFGNSAVVL
Sbjct: 361 GPSKHQAVALMSTSDQSIFYRCQMNAYQDTLYAHSNRQFYRDCKVYGTVDFIFGNSAVVL 420

Query: 421 QNCTIAPRLPLLGQKNTITAQGRFDPNQNTGISIQGSLIQPFNDLKTTETYLGRPWKNYS 480
           QNC IAP+LPLLGQKNTITAQGRFDPNQ+TGISIQ S IQPF +LKTT+TYLGRPWKNYS
Sbjct: 421 QNCKIAPKLPLLGQKNTITAQGRFDPNQHTGISIQHSSIQPFENLKTTQTYLGRPWKNYS 480

Query: 481 TTVFMQSNLGRLIHPTGWLPWIGTSAPDTIFYAEFQNYGPGSSTSKRVKWKGVKNIDTKT 540
           TTVFMQ+N G LIHPTGWLPW+GTSAPDTIFYAEFQN GPGSST+ RVKWKG+KNIDTKT
Sbjct: 481 TTVFMQNNFGSLIHPTGWLPWVGTSAPDTIFYAEFQNKGPGSSTANRVKWKGLKNIDTKT 540

Query: 541 AKKFTVSSFIDGKDWISKAQVPFTATL 568
           AKKFTVSSFIDGKDWISKAQVPF ATL
Sbjct: 541 AKKFTVSSFIDGKDWISKAQVPFNATL 566

BLAST of CsaV3_1G037280 vs. NCBI nr
Match: XP_023520176.1 (putative pectinesterase/pectinesterase inhibitor 24 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 896.3 bits (2315), Expect = 5.2e-257
Identity = 477/567 (84.13%), Postives = 518/567 (91.36%), Query Frame = 0

Query: 1   MSFLRPYGKVDETDQLRLDARXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 60
           MS LRPYGKVDE+DQ+RLDA      XXXXXXXXXXXXXXXXXXXXXXXXXXXX      
Sbjct: 1   MSLLRPYGKVDESDQMRLDA-SRRTRXXXXXXXXXXXXXXXXXXXXXXXXXXXXKNDESD 60

Query: 61  XXXXSLSTSIKAVCDVTLYPDTCQKAFGPIANSSHLDPGQISKLSVQLALGELSKVADYI 120
               +LSTSIKAVCD TLYPDTC+ AFG +ANSSHLDPGQI+K SVQLALGELS+VADY+
Sbjct: 61  NHNEALSTSIKAVCDETLYPDTCKNAFGSLANSSHLDPGQIAKFSVQLALGELSRVADYL 120

Query: 121 FDHAITKTADNKTILALKNCHELLDLALDHLNISLSSSDITLLKAVDDLKTWITSAATYQ 180
            +HAIT + DNKTILAL++CHELL+LA+DHLN SLSSS+IT+LKAVDDLKTW++SAATYQ
Sbjct: 121 LEHAITNSTDNKTILALRSCHELLELAIDHLNDSLSSSEITVLKAVDDLKTWLSSAATYQ 180

Query: 181 QTCIDDLAEVDPALADLVANFLKNSTELTSNGLAIVSFFSKLTDSLSLRRLMSYENHQSN 240
           QTCID L EVD AL +++AN+LKNSTE+TSNGLAIVSFFSKLTDS SLRRLMSY++  SN
Sbjct: 181 QTCIDGLEEVDLALTNVIANYLKNSTEMTSNGLAIVSFFSKLTDSFSLRRLMSYDDRHSN 240

Query: 241 GDWARPILRKLAQSDIRKHADIVVAKDRSGKYKTITEALGAVPDKSKKRTVIYVKKGIYK 300
           GDWARPILRKLAQ D+RK ADIVVAKD+SGKYKTITEAL AVPDKSKKRTVIYVKKG+YK
Sbjct: 241 GDWARPILRKLAQKDLRKQADIVVAKDKSGKYKTITEALNAVPDKSKKRTVIYVKKGVYK 300

Query: 301 ENVEVVKNKWNVVMVGDGMTATIVSGNLNVVDGTPTFSTATFAAKGKGFIAIDMGFINTA 360
           ENVEVVKNKWNVVM+GDGMT T V+G LN +DGTPTFSTATFAAKGKGFIAIDMGF NTA
Sbjct: 301 ENVEVVKNKWNVVMIGDGMTLTTVTGRLNFIDGTPTFSTATFAAKGKGFIAIDMGFENTA 360

Query: 361 GPSKHQAVALMSTSDQSIFYRCEMNAYQDTLYAHSNRQFYRECKVYGTVDFIFGNSAVVL 420
           GPSKHQAVALMSTSDQSIFYRC+MNAYQDTLYAHSNRQFYR+CKVYGTVDFIFGNSAVVL
Sbjct: 361 GPSKHQAVALMSTSDQSIFYRCQMNAYQDTLYAHSNRQFYRDCKVYGTVDFIFGNSAVVL 420

Query: 421 QNCTIAPRLPLLGQKNTITAQGRFDPNQNTGISIQGSLIQPFNDLKTTETYLGRPWKNYS 480
           QNC IAP+LPLLGQKNTITAQGRFDPNQ+TGISIQ S IQPF +LKTTETYLGRPWKNYS
Sbjct: 421 QNCKIAPKLPLLGQKNTITAQGRFDPNQHTGISIQHSSIQPFENLKTTETYLGRPWKNYS 480

Query: 481 TTVFMQSNLGRLIHPTGWLPWIGTSAPDTIFYAEFQNYGPGSSTSKRVKWKGVKNIDTKT 540
           TTVFMQ++ GRLIHPTGWLPW+GTSAPDTIFYAEFQN GPGSST+ RVKWKG+KNIDTKT
Sbjct: 481 TTVFMQNDFGRLIHPTGWLPWVGTSAPDTIFYAEFQNKGPGSSTANRVKWKGLKNIDTKT 540

Query: 541 AKKFTVSSFIDGKDWISKAQVPFTATL 568
           AKKFTVSSFI GKDWISKAQVPF ATL
Sbjct: 541 AKKFTVSSFIHGKDWISKAQVPFNATL 566

BLAST of CsaV3_1G037280 vs. TAIR10
Match: AT3G10710.1 (root hair specific 12)

HSP 1 Score: 552.4 bits (1422), Expect = 3.3e-157
Identity = 296/568 (52.11%), Postives = 376/568 (66.20%), Query Frame = 0

Query: 6   PYGKVDETDQLRLDAR--XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 65
           PYGKVDE + +RL+AR                                            
Sbjct: 4   PYGKVDEREHVRLEARRKTRKNIAIIAVSLVILAGIVIGAVFGTMAHKKSPETVETNNNG 63

Query: 66  XSLSTSIKAVCDVTLYPDTCQKAFGPIANSSHLDPGQISKLSVQLALGELSKVADYIFDH 125
            S+S S+KAVCDVTL+ + C +  G   N+S L+P ++ + +V++ + E+SK  +     
Sbjct: 64  DSISVSVKAVCDVTLHKEKCFETLGSAPNASSLNPEELFRYAVKITIAEVSKAINAF--- 123

Query: 126 AITKTADNKTILALKNCHELLDLALDHLNISLSSS---DITLLKAVDDLKTWITSAATYQ 185
             +   D K  + +  C ELLDL +D+LN +L+SS   D+T+ + VDDL+TW++SA TYQ
Sbjct: 124 -SSSLGDEKNNITMNACAELLDLTIDNLNNTLTSSSNGDVTVPELVDDLRTWLSSAGTYQ 183

Query: 186 QTCIDDLAEVDPALADLVANFLKNSTELTSNGLAIVSFFSKLTDSLSL-RRLMSYENHQS 245
           +TC++ LA   P +     + LKNSTELTSN LAI+++  K+ DS  L RRL++  + + 
Sbjct: 184 RTCVETLA---PDMRPFGESHLKNSTELTSNALAIITWLGKIADSFKLRRRLLTTADVEV 243

Query: 246 NGDWARPILRKLAQSDIRKHADIVVAKDRSGKYKTITEALGAVPDKSKKRTVIYVKKGIY 305
           +    R   R L  +D+RK ADIVVAKD SGKY+TI  AL  VP+KS+KRT+IYVKKG+Y
Sbjct: 244 DFHAGR---RLLQSTDLRKVADIVVAKDGSGKYRTIKRALQDVPEKSEKRTIIYVKKGVY 303

Query: 306 KENVEVVKNKWNVVMVGDGMTATIVSGNLNVVDGTPTFSTATFAAKGKGFIAIDMGFINT 365
            ENV+V K  WNV++VGDG + +IVSG LNV+DGTPTF TATFA  GKGF+A DMGFINT
Sbjct: 304 FENVKVEKKMWNVIVVGDGESKSIVSGRLNVIDGTPTFKTATFAVFGKGFMARDMGFINT 363

Query: 366 AGPSKHQAVALMSTSDQSIFYRCEMNAYQDTLYAHSNRQFYRECKVYGTVDFIFGNSAVV 425
           AGPSKHQAVALM ++D + FYRC MNAYQDTLY H+ RQFYREC + GTVDFIFGNSA V
Sbjct: 364 AGPSKHQAVALMVSADLTAFYRCTMNAYQDTLYVHAQRQFYRECTIIGTVDFIFGNSASV 423

Query: 426 LQNCTIAPRLPLLGQKNTITAQGRFDPNQNTGISIQGSLIQPFNDLKTTETYLGRPWKNY 485
           LQ+C I PR P+ GQ+NTITAQGR DPN NTGISI    I P  DL    T+LGRPWKN+
Sbjct: 424 LQSCRILPRRPMKGQQNTITAQGRTDPNMNTGISIHRCNISPLGDLTDVMTFLGRPWKNF 483

Query: 486 STTVFMQSNLGRLIHPTGWLPWIGTSAPDTIFYAEFQNYGPGSSTSKRVKWKGVKNIDTK 545
           STTV M S L   I   GWLPW G SAPDTIFY E++N GPG+ST  RVKWKG++ + TK
Sbjct: 484 STTVIMDSYLHGFIDRKGWLPWTGDSAPDTIFYGEYKNTGPGASTKNRVKWKGLRFLSTK 543

Query: 546 TAKKFTVSSFIDGKDWISKAQVPFTATL 568
            A +FTV  FIDG  W+   +VPF + L
Sbjct: 544 EANRFTVKPFIDGGRWLPATKVPFRSGL 561

BLAST of CsaV3_1G037280 vs. TAIR10
Match: AT5G04960.1 (Plant invertase/pectin methylesterase inhibitor superfamily)

HSP 1 Score: 526.6 bits (1355), Expect = 2.0e-149
Identity = 272/506 (53.75%), Postives = 353/506 (69.76%), Query Frame = 0

Query: 66  LSTSIKAVCDVTLYPDTCQKAFGPIANSSHLDPGQISKLSVQLALGELSKVADYIFDHAI 125
           +S S+KA+CDVTL+ + C +  G   N+S   P ++ K +V++ + ELSKV D     + 
Sbjct: 64  ISVSVKALCDVTLHKEKCFETLGSAPNASRSSPEELFKYAVKVTITELSKVLD---GFSN 123

Query: 126 TKTADNKTILALKNCHELLDLALDHLNISLSSSDITLLKAVDDLKTWITSAATYQQTCID 185
            +  DN T  A+  C EL+ LA+D LN +++SS    LK  DDL+TW++S  TYQ+TC+D
Sbjct: 124 GEHMDNATSAAMGACVELIGLAVDQLNETMTSS----LKNFDDLRTWLSSVGTYQETCMD 183

Query: 186 DLAEVD-PALADLVANFLKNSTELTSNGLAIVSFFSKLTDSLSLRRLMSYENHQSNGDWA 245
            L E + P+L     N LKNSTE+TSN LAI+++  K+ D++  RR    E   +    A
Sbjct: 184 ALVEANKPSLTTFGENHLKNSTEMTSNALAIITWLGKIADTVKFRRRRLLETGNAKVVVA 243

Query: 246 ----RPILRKLAQSDIRKHADIVVAKDRSGKYKTITEALGAVPDKSKKRTVIYVKKGIYK 305
                            K A IVVAKD SGKY+TI EAL  V +K++K T+IYVKKG+Y 
Sbjct: 244 DLXXXXXXXXXXXXXXXKKATIVVAKDGSGKYRTIGEALAEVEEKNEKPTIIYVKKGVYL 303

Query: 306 ENVEVVKNKWNVVMVGDGMTATIVSGNLNVVDGTPTFSTATFAAKGKGFIAIDMGFINTA 365
           ENV V K KWNVVMVGDG + TIVS  LN +DGTPTF TATFA  GKGF+A DMGFINTA
Sbjct: 304 ENVRVEKTKWNVVMVGDGQSKTIVSAGLNFIDGTPTFETATFAVFGKGFMARDMGFINTA 363

Query: 366 GPSKHQAVALMSTSDQSIFYRCEMNAYQDTLYAHSNRQFYRECKVYGTVDFIFGNSAVVL 425
           GP+KHQAVALM ++D S+FY+C M+A+QDT+YAH+ RQFYR+C + GTVDFIFGN+AVV 
Sbjct: 364 GPAKHQAVALMVSADLSVFYKCTMDAFQDTMYAHAQRQFYRDCVILGTVDFIFGNAAVVF 423

Query: 426 QNCTIAPRLPLLGQKNTITAQGRFDPNQNTGISIQGSLIQPFNDLKTTETYLGRPWKNYS 485
           Q C I PR P+ GQ+NTITAQGR DPNQNTGISI    I+P ++L   +T+LGRPWK++S
Sbjct: 424 QKCEILPRRPMKGQQNTITAQGRKDPNQNTGISIHNCTIKPLDNLTDIQTFLGRPWKDFS 483

Query: 486 TTVFMQSNLGRLIHPTGWLPWIGTSAPDTIFYAEFQNYGPGSSTSKRVKWKGVK-NIDTK 545
           TTV M+S + + I+P GWLPW G +APDTIFYAE+ N GPG+ST  RVKW+G+K ++  K
Sbjct: 484 TTVIMKSFMDKFINPKGWLPWTGDTAPDTIFYAEYLNSGPGASTKNRVKWQGLKTSLTKK 543

Query: 546 TAKKFTVSSFIDGKDWISKAQVPFTA 566
            A KFTV  FIDG +W+   +VPF +
Sbjct: 544 EANKFTVKPFIDGNNWLPATKVPFNS 562

BLAST of CsaV3_1G037280 vs. TAIR10
Match: AT1G53840.1 (pectin methylesterase 1)

HSP 1 Score: 406.0 bits (1042), Expect = 3.9e-113
Identity = 230/513 (44.83%), Postives = 319/513 (62.18%), Query Frame = 0

Query: 67  STSIKAVCDVTLYPDTCQKAFGPIANSSHLDPGQISKLSVQLALGELSKVADYIFDHAIT 126
           STS+KA+C VT +P++C  +   + +S+  DP  + KLS+++ + EL  ++D + +    
Sbjct: 70  STSLKAICSVTRFPESCISSISKLPSSNTTDPETLFKLSLKVIIDELDSISD-LPEKLSK 129

Query: 127 KTADNKTILALKNCHELLDLALDHLNISLSSSDITLLK------AVDDLKTWITSAATYQ 186
           +T D +   AL+ C +L++ ALD LN ++S+ D    K       ++DLKTW+++  T  
Sbjct: 130 ETEDERIKSALRVCGDLIEDALDRLNDTVSAIDDEEKKKTLSSSKIEDLKTWLSATVTDH 189

Query: 187 QTCIDDLAEV--------DPALADLVANFLKNSTELTSNGLAIVS-FFSKLTD-SLSLRR 246
           +TC D L E+        +  +   + + +  STE TSN LAIVS   S L+D  + + R
Sbjct: 190 ETCFDSLDELKQNKTEYANSTITQNLKSAMSRSTEFTSNSLAIVSKILSALSDLGIPIHR 249

Query: 247 LMSYENHQSNGD-----WARPILRKLAQSDIRKHADIVVAKDRSGKYKTITEALGAVPDK 306
                            WAR   R+L Q+   K  D+ VA D +G   T+ EA+  VP K
Sbjct: 250 XXXXXXXXXXXSVDFEKWAR---RRLLQTAGLK-PDVTVAGDGTGDVLTVNEAVAKVPKK 309

Query: 307 SKKRTVIYVKKGIYKENVEVVKNKWNVVMVGDGMTATIVSGNLNVVDGTPTFSTATFAAK 366
           S K  VIYVK G Y ENV + K+KWNV++ GDG   TI+SG+ N VDGTPT+ TATFA +
Sbjct: 310 SLKMFVIYVKSGTYVENVVMDKSKWNVMIYGDGKGKTIISGSKNFVDGTPTYETATFAIQ 369

Query: 367 GKGFIAIDMGFINTAGPSKHQAVALMSTSDQSIFYRCEMNAYQDTLYAHSNRQFYRECKV 426
           GKGFI  D+G INTAG +KHQAVA  S SD S++Y+C  + +QDTLY HSNRQFYR+C V
Sbjct: 370 GKGFIMKDIGIINTAGAAKHQAVAFRSGSDFSVYYQCSFDGFQDTLYPHSNRQFYRDCDV 429

Query: 427 YGTVDFIFGNSAVVLQNCTIAPRLPLLGQKNTITAQGRFDPNQNTGISIQGSLIQPFNDL 486
            GT+DFIFG++AVV Q C I PR PL  Q NTITAQG+ DPNQ++G+SIQ   I   N  
Sbjct: 430 TGTIDFIFGSAAVVFQGCKIMPRQPLSNQFNTITAQGKKDPNQSSGMSIQRCTISA-NGN 489

Query: 487 KTTETYLGRPWKNYSTTVFMQSNLGRLIHPTGWLPWI-GTSAPDTIFYAEFQNYGPGSST 546
               TYLGRPWK +STTV M++ +G ++ P+GW+ W+ G   P +I Y E++N GPGS  
Sbjct: 490 VIAPTYLGRPWKEFSTTVIMETVIGAVVRPSGWMSWVSGVDPPASIVYGEYKNTGPGSDV 549

Query: 547 SKRVKWKGVKNI-DTKTAKKFTVSSFIDGKDWI 557
           ++RVKW G K +     A KFTV++ + G DWI
Sbjct: 550 TQRVKWAGYKPVMSDAEAAKFTVATLLHGADWI 576

BLAST of CsaV3_1G037280 vs. TAIR10
Match: AT3G14300.1 (pectinesterase family protein)

HSP 1 Score: 396.0 bits (1016), Expect = 4.0e-110
Identity = 237/513 (46.20%), Postives = 310/513 (60.43%), Query Frame = 0

Query: 67  STSIKAVCDVTLYPDTCQKAFGPI-ANSSHLDPGQISKLSVQLALGELSKVADYIFDHAI 126
           S+ ++ VC+VT YP +C  +   +  + +  DP  + +LS+Q+   EL+ +   +     
Sbjct: 456 SSVLRTVCNVTNYPASCISSISKLPLSKTTTDPKVLFRLSLQVTFDELNSIVG-LPKKLA 515

Query: 127 TKTADNKTILALKNCHELLDLALDHLNISLSSSDITL--------LKAVDDLKTWITSAA 186
            +T D     AL  C ++ DLA+D +N ++SS D  +           + DL TW++SA 
Sbjct: 516 EETNDEGLKSALSVCADVFDLAVDSVNDTISSLDEVISGGKKNLNSSTIGDLITWLSSAV 575

Query: 187 TYQQTCIDDLAE--VDPALADLVANFLKNSTELTSNGLAIVSFF----SKLTDSLSLRRL 246
           T   TC D L E   +  +   + + + NSTE TSN LAIV+      SK    +  RRL
Sbjct: 576 TDIGTCGDTLDEDNYNSPIPQKLKSAMVNSTEFTSNSLAIVAQVLKKPSKSRIPVQGRRL 635

Query: 247 MSYENHQSNGDWARPILRKLAQS-DIRKHADIVVAKDRSGKYKTITEALGAVPDKSKKRT 306
           +   N  S  +W RP +R+L Q+ ++  H  + VA D SG  +T+ EA+  VP K K   
Sbjct: 636 L---NSNSFPNWVRPGVRRLLQAKNLTPH--VTVAADGSGDVRTVNEAVWRVPKKGKTMF 695

Query: 307 VIYVKKGIYKENVEVVKNKWNVVMVGDGMTATIVSGNLNVVDGTPTFSTATFAAKGKGFI 366
           VIYVK G Y ENV + K+KWNV + GDG   TI+SG+ N+VDG  TF+T+TFA +GKGF+
Sbjct: 696 VIYVKAGTYVENVLMKKDKWNVFIYGDGRDKTIISGSTNMVDGVRTFNTSTFATEGKGFM 755

Query: 367 AIDMGFINTAGPSKHQAVALMSTSDQSIFYRCEMNAYQDTLYAHSNRQFYRECKVYGTVD 426
             DMG INTAGP KHQAVA  S SD+S++YRC  + YQDTLY HSNRQ+YR C V GTVD
Sbjct: 756 MKDMGIINTAGPEKHQAVAFRSDSDRSVYYRCSFDGYQDTLYTHSNRQYYRNCDVTGTVD 815

Query: 427 FIFGNSAVVLQNCTIAPRLPLLGQKNTITAQGRFDPNQNTGISIQGSLIQPFNDLKTTET 486
           FIFG   VV Q C+I PR PL  Q NTITA+G  + NQNTGISI    I P N   T  T
Sbjct: 816 FIFGAGTVVFQGCSIRPRQPLPNQFNTITAEGTQEANQNTGISIHQCTISP-NGNVTATT 875

Query: 487 YLGRPWKNYSTTVFMQSNLGRLIHPTGWLPWIGT--SAPDTIFYAEFQNYGPGSSTSKRV 546
           YLGRPWK +S TV MQS +G  ++P GW+ W  T    P TIFY E++N GPGS  SKRV
Sbjct: 876 YLGRPWKLFSKTVIMQSVIGSFVNPAGWIAWNSTYDPPPRTIFYREYKNSGPGSDLSKRV 935

Query: 547 KWKGVKNIDT-KTAKKFTVSSFIDGKD-WISKA 560
           KW G K I +   A +FTV  F+ G D WI KA
Sbjct: 936 KWAGYKPISSDDEAARFTVKYFLRGDDNWIPKA 961

BLAST of CsaV3_1G037280 vs. TAIR10
Match: AT3G49220.1 (Plant invertase/pectin methylesterase inhibitor superfamily)

HSP 1 Score: 374.0 bits (959), Expect = 1.6e-103
Identity = 227/525 (43.24%), Postives = 312/525 (59.43%), Query Frame = 0

Query: 67  STSIKAVCDVTLYPDTCQKAF----GPIANSSHLDPGQISKLSVQLALGELSKVADYIFD 126
           S +I   C++T +P+ C  +     G +A SS  D   +  ++V + L        + F 
Sbjct: 84  SQAISKACELTRFPELCVDSLMDFPGSLAASSSKD---LIHVTVNMTL--------HHFS 143

Query: 127 HAITKTADNKTI-------LALKNCHELLDLALDHLNISLSSSDITLLKAVDDLKTWITS 186
           HA+  +A    +        A  +C ELLD ++D L+ +LSS   +  K   D+ TW+++
Sbjct: 144 HALYSSASLSFVDMPPRARSAYDSCVELLDDSVDALSRALSSVVSSSAKP-QDVTTWLSA 203

Query: 187 AATYQQTCIDDLAEVDP-ALADLVANFLKNSTELTSNGLAIVSFFSKLTD----SLSLRR 246
           A T   TC +    VD   + D +   L+N +EL SN LAI S      D     +  RR
Sbjct: 204 ALTNHDTCTEGFDGVDDGGVKDHMTAALQNLSELVSNCLAIFSASHDGDDFAGVPIQNRR 263

Query: 247 LMSYENHQSN-GDWARPILRKLAQSDIRK-HADIVVAKDRSGKYKTITEALGAVPDKSKK 306
           L+  E  +     W RP  R++ +  + +  ADI+V+KD +G  KTI+EA+   P  S +
Sbjct: 264 LLGVEEREEKFPRWMRPKEREILEMPVSQIQADIIVSKDGNGTCKTISEAIKKAPQNSTR 323

Query: 307 RTVIYVKKGIYKE-NVEVVKNKWNVVMVGDGMTATIVSGNLNVVDGTPTFSTATFAAKGK 366
           R +IYVK G Y+E N++V + K N++ VGDG   T++SG  ++ D   TF TA+FAA G 
Sbjct: 324 RIIIYVKAGRYEENNLKVGRKKINLMFVGDGKGKTVISGGKSIFDNITTFHTASFAATGA 383

Query: 367 GFIAIDMGFINTAGPSKHQAVALMSTSDQSIFYRCEMNAYQDTLYAHSNRQFYRECKVYG 426
           GFIA D+ F N AGP+KHQAVAL   +D ++ YRC +  YQDTLY HSNRQF+REC +YG
Sbjct: 384 GFIARDITFENWAGPAKHQAVALRIGADHAVIYRCNIIGYQDTLYVHSNRQFFRECDIYG 443

Query: 427 TVDFIFGNSAVVLQNCTIAPRLPLLGQKNTITAQGRFDPNQNTGISIQGSLIQPFNDLK- 486
           TVDFIFGN+AVVLQNC+I  R P+  QKNTITAQ R DPNQNTGISI  S +   +DL+ 
Sbjct: 444 TVDFIFGNAAVVLQNCSIYARKPMDFQKNTITAQNRKDPNQNTGISIHASRVLAASDLQA 503

Query: 487 ---TTETYLGRPWKNYSTTVFMQSNLGRLIHPTGWLPWIGTSAPDTIFYAEFQNYGPGSS 546
              +T+TYLGRPWK +S TV+M S +G  +H  GWL W  T A DT++Y E+ N GPGS 
Sbjct: 504 TNGSTQTYLGRPWKLFSRTVYMMSYIGGHVHTRGWLEWNTTFALDTLYYGEYLNSGPGSG 563

Query: 547 TSKRVKWKGVKNID-TKTAKKFTVSSFIDGKDWISKAQVPFTATL 568
             +RV W G + I+ T  A +FTV+ FI G  W+    V F A L
Sbjct: 564 LGQRVSWPGYRVINSTAEANRFTVAEFIYGSSWLPSTGVSFLAGL 596

BLAST of CsaV3_1G037280 vs. Swiss-Prot
Match: sp|Q9SG77|PME24_ARATH (Putative pectinesterase/pectinesterase inhibitor 24 OS=Arabidopsis thaliana OX=3702 GN=PME24 PE=3 SV=1)

HSP 1 Score: 552.4 bits (1422), Expect = 6.0e-156
Identity = 296/568 (52.11%), Postives = 376/568 (66.20%), Query Frame = 0

Query: 6   PYGKVDETDQLRLDAR--XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 65
           PYGKVDE + +RL+AR                                            
Sbjct: 4   PYGKVDEREHVRLEARRKTRKNIAIIAVSLVILAGIVIGAVFGTMAHKKSPETVETNNNG 63

Query: 66  XSLSTSIKAVCDVTLYPDTCQKAFGPIANSSHLDPGQISKLSVQLALGELSKVADYIFDH 125
            S+S S+KAVCDVTL+ + C +  G   N+S L+P ++ + +V++ + E+SK  +     
Sbjct: 64  DSISVSVKAVCDVTLHKEKCFETLGSAPNASSLNPEELFRYAVKITIAEVSKAINAF--- 123

Query: 126 AITKTADNKTILALKNCHELLDLALDHLNISLSSS---DITLLKAVDDLKTWITSAATYQ 185
             +   D K  + +  C ELLDL +D+LN +L+SS   D+T+ + VDDL+TW++SA TYQ
Sbjct: 124 -SSSLGDEKNNITMNACAELLDLTIDNLNNTLTSSSNGDVTVPELVDDLRTWLSSAGTYQ 183

Query: 186 QTCIDDLAEVDPALADLVANFLKNSTELTSNGLAIVSFFSKLTDSLSL-RRLMSYENHQS 245
           +TC++ LA   P +     + LKNSTELTSN LAI+++  K+ DS  L RRL++  + + 
Sbjct: 184 RTCVETLA---PDMRPFGESHLKNSTELTSNALAIITWLGKIADSFKLRRRLLTTADVEV 243

Query: 246 NGDWARPILRKLAQSDIRKHADIVVAKDRSGKYKTITEALGAVPDKSKKRTVIYVKKGIY 305
           +    R   R L  +D+RK ADIVVAKD SGKY+TI  AL  VP+KS+KRT+IYVKKG+Y
Sbjct: 244 DFHAGR---RLLQSTDLRKVADIVVAKDGSGKYRTIKRALQDVPEKSEKRTIIYVKKGVY 303

Query: 306 KENVEVVKNKWNVVMVGDGMTATIVSGNLNVVDGTPTFSTATFAAKGKGFIAIDMGFINT 365
            ENV+V K  WNV++VGDG + +IVSG LNV+DGTPTF TATFA  GKGF+A DMGFINT
Sbjct: 304 FENVKVEKKMWNVIVVGDGESKSIVSGRLNVIDGTPTFKTATFAVFGKGFMARDMGFINT 363

Query: 366 AGPSKHQAVALMSTSDQSIFYRCEMNAYQDTLYAHSNRQFYRECKVYGTVDFIFGNSAVV 425
           AGPSKHQAVALM ++D + FYRC MNAYQDTLY H+ RQFYREC + GTVDFIFGNSA V
Sbjct: 364 AGPSKHQAVALMVSADLTAFYRCTMNAYQDTLYVHAQRQFYRECTIIGTVDFIFGNSASV 423

Query: 426 LQNCTIAPRLPLLGQKNTITAQGRFDPNQNTGISIQGSLIQPFNDLKTTETYLGRPWKNY 485
           LQ+C I PR P+ GQ+NTITAQGR DPN NTGISI    I P  DL    T+LGRPWKN+
Sbjct: 424 LQSCRILPRRPMKGQQNTITAQGRTDPNMNTGISIHRCNISPLGDLTDVMTFLGRPWKNF 483

Query: 486 STTVFMQSNLGRLIHPTGWLPWIGTSAPDTIFYAEFQNYGPGSSTSKRVKWKGVKNIDTK 545
           STTV M S L   I   GWLPW G SAPDTIFY E++N GPG+ST  RVKWKG++ + TK
Sbjct: 484 STTVIMDSYLHGFIDRKGWLPWTGDSAPDTIFYGEYKNTGPGASTKNRVKWKGLRFLSTK 543

Query: 546 TAKKFTVSSFIDGKDWISKAQVPFTATL 568
            A +FTV  FIDG  W+   +VPF + L
Sbjct: 544 EANRFTVKPFIDGGRWLPATKVPFRSGL 561

BLAST of CsaV3_1G037280 vs. Swiss-Prot
Match: sp|Q9FF78|PME46_ARATH (Probable pectinesterase/pectinesterase inhibitor 46 OS=Arabidopsis thaliana OX=3702 GN=PME46 PE=2 SV=1)

HSP 1 Score: 526.6 bits (1355), Expect = 3.5e-148
Identity = 272/506 (53.75%), Postives = 353/506 (69.76%), Query Frame = 0

Query: 66  LSTSIKAVCDVTLYPDTCQKAFGPIANSSHLDPGQISKLSVQLALGELSKVADYIFDHAI 125
           +S S+KA+CDVTL+ + C +  G   N+S   P ++ K +V++ + ELSKV D     + 
Sbjct: 64  ISVSVKALCDVTLHKEKCFETLGSAPNASRSSPEELFKYAVKVTITELSKVLD---GFSN 123

Query: 126 TKTADNKTILALKNCHELLDLALDHLNISLSSSDITLLKAVDDLKTWITSAATYQQTCID 185
            +  DN T  A+  C EL+ LA+D LN +++SS    LK  DDL+TW++S  TYQ+TC+D
Sbjct: 124 GEHMDNATSAAMGACVELIGLAVDQLNETMTSS----LKNFDDLRTWLSSVGTYQETCMD 183

Query: 186 DLAEVD-PALADLVANFLKNSTELTSNGLAIVSFFSKLTDSLSLRRLMSYENHQSNGDWA 245
            L E + P+L     N LKNSTE+TSN LAI+++  K+ D++  RR    E   +    A
Sbjct: 184 ALVEANKPSLTTFGENHLKNSTEMTSNALAIITWLGKIADTVKFRRRRLLETGNAKVVVA 243

Query: 246 ----RPILRKLAQSDIRKHADIVVAKDRSGKYKTITEALGAVPDKSKKRTVIYVKKGIYK 305
                            K A IVVAKD SGKY+TI EAL  V +K++K T+IYVKKG+Y 
Sbjct: 244 DLXXXXXXXXXXXXXXXKKATIVVAKDGSGKYRTIGEALAEVEEKNEKPTIIYVKKGVYL 303

Query: 306 ENVEVVKNKWNVVMVGDGMTATIVSGNLNVVDGTPTFSTATFAAKGKGFIAIDMGFINTA 365
           ENV V K KWNVVMVGDG + TIVS  LN +DGTPTF TATFA  GKGF+A DMGFINTA
Sbjct: 304 ENVRVEKTKWNVVMVGDGQSKTIVSAGLNFIDGTPTFETATFAVFGKGFMARDMGFINTA 363

Query: 366 GPSKHQAVALMSTSDQSIFYRCEMNAYQDTLYAHSNRQFYRECKVYGTVDFIFGNSAVVL 425
           GP+KHQAVALM ++D S+FY+C M+A+QDT+YAH+ RQFYR+C + GTVDFIFGN+AVV 
Sbjct: 364 GPAKHQAVALMVSADLSVFYKCTMDAFQDTMYAHAQRQFYRDCVILGTVDFIFGNAAVVF 423

Query: 426 QNCTIAPRLPLLGQKNTITAQGRFDPNQNTGISIQGSLIQPFNDLKTTETYLGRPWKNYS 485
           Q C I PR P+ GQ+NTITAQGR DPNQNTGISI    I+P ++L   +T+LGRPWK++S
Sbjct: 424 QKCEILPRRPMKGQQNTITAQGRKDPNQNTGISIHNCTIKPLDNLTDIQTFLGRPWKDFS 483

Query: 486 TTVFMQSNLGRLIHPTGWLPWIGTSAPDTIFYAEFQNYGPGSSTSKRVKWKGVK-NIDTK 545
           TTV M+S + + I+P GWLPW G +APDTIFYAE+ N GPG+ST  RVKW+G+K ++  K
Sbjct: 484 TTVIMKSFMDKFINPKGWLPWTGDTAPDTIFYAEYLNSGPGASTKNRVKWQGLKTSLTKK 543

Query: 546 TAKKFTVSSFIDGKDWISKAQVPFTA 566
            A KFTV  FIDG +W+   +VPF +
Sbjct: 544 EANKFTVKPFIDGNNWLPATKVPFNS 562

BLAST of CsaV3_1G037280 vs. Swiss-Prot
Match: sp|Q43111|PME3_PHAVU (Pectinesterase 3 OS=Phaseolus vulgaris OX=3885 GN=MPE3 PE=2 SV=1)

HSP 1 Score: 452.6 bits (1163), Expect = 6.5e-126
Identity = 248/519 (47.78%), Postives = 332/519 (63.97%), Query Frame = 0

Query: 65  SLSTSIKAVCDVTLYPDTCQKAFGPIANSSHLDPGQISKLSVQLALGELSKVADYIFDHA 124
           S + S+KAVCD T YP +C  +   +  S+  DP  + KLS+++A+ ELS     +  +A
Sbjct: 72  SPAASLKAVCDTTRYPSSCFSSISSLPESNTTDPELLFKLSLRVAIDELSSFPSKLRANA 131

Query: 125 ITKTADNKTILALKNCHELLDLALDHLNISLS-----SSDITLLKAVDDLKTWITSAATY 184
                D +   A+  C  +   ALD LN S+S     +  I    +V +++TW+++A T 
Sbjct: 132 ---EQDARLQKAIDVCSSVFGDALDRLNDSISALGTVAGRIASSASVSNVETWLSAALTD 191

Query: 185 QQTCIDDLAEVDPALA----DLVANFLKNSTELTSNGLAIVS----FFSKLTDSLSLRRL 244
           Q TC+D + E++   A      +   ++NSTE  SN LAIV+      S+    +  RRL
Sbjct: 192 QDTCLDAVGELNSTAARGALQEIETAMRNSTEFASNSLAIVTKILGLLSRFETPIHHRRL 251

Query: 245 MSYENHQSNGDWARPILRKLAQSDIRKHA-DIVVAKDRSGKYKTITEALGAVPDKSKKRT 304
           + +       +W     R+L +        D VVAKD SG++KTI EAL  V  KS++R 
Sbjct: 252 LGFP------EWLGAAERRLLEEKNNDSTPDAVVAKDGSGQFKTIGEALKLVKKKSEERF 311

Query: 305 VIYVKKGIYKENVEVVKNKWNVVMVGDGMTATIVSGNLNVVDGTPTFSTATFAAKGKGFI 364
            +YVK+G Y EN+++ KN WNV++ GDG   T V G+ N +DGTPTF TATFA KGKGFI
Sbjct: 312 SVYVKEGRYVENIDLDKNTWNVMIYGDGKDKTFVVGSRNFMDGTPTFETATFAVKGKGFI 371

Query: 365 AIDMGFINTAGPSKHQAVALMSTSDQSIFYRCEMNAYQDTLYAHSNRQFYRECKVYGTVD 424
           A D+GF+N AG SKHQAVAL S SD+S+F+RC  + +QDTLYAHSNRQFYR+C + GT+D
Sbjct: 372 AKDIGFVNNAGASKHQAVALRSGSDRSVFFRCSFDGFQDTLYAHSNRQFYRDCDITGTID 431

Query: 425 FIFGNSAVVLQNCTIAPRLPLLGQKNTITAQGRFDPNQNTGISIQGSLIQPFNDLKTTET 484
           FIFGN+AVV Q+C I PR PL  Q NTITAQG+ DPNQNTGI IQ S I PF +  T  T
Sbjct: 432 FIFGNAAVVFQSCKIMPRQPLPNQFNTITAQGKKDPNQNTGIIIQKSTITPFGNNLTAPT 491

Query: 485 YLGRPWKNYSTTVFMQSNLGRLIHPTGWLPWI-GTSAPDTIFYAEFQNYGPGSSTSKRVK 544
           YLGRPWK++STTV MQS++G L++P GW+ W+     P TIFYAE+QN GPG+  S+RVK
Sbjct: 492 YLGRPWKDFSTTVIMQSDIGALLNPVGWMSWVPNVEPPTTIFYAEYQNSGPGADVSQRVK 551

Query: 545 WKGVK-NIDTKTAKKFTVSSFIDGKDWISKAQVPFTATL 568
           W G K  I  + A++FTV SFI G +W+  A V F +TL
Sbjct: 552 WAGYKPTITDRNAEEFTVQSFIQGPEWLPNAAVQFDSTL 581

BLAST of CsaV3_1G037280 vs. Swiss-Prot
Match: sp|Q43867|PME1_ARATH (Pectinesterase 1 OS=Arabidopsis thaliana OX=3702 GN=PME1 PE=1 SV=1)

HSP 1 Score: 406.0 bits (1042), Expect = 6.9e-112
Identity = 230/513 (44.83%), Postives = 319/513 (62.18%), Query Frame = 0

Query: 67  STSIKAVCDVTLYPDTCQKAFGPIANSSHLDPGQISKLSVQLALGELSKVADYIFDHAIT 126
           STS+KA+C VT +P++C  +   + +S+  DP  + KLS+++ + EL  ++D + +    
Sbjct: 70  STSLKAICSVTRFPESCISSISKLPSSNTTDPETLFKLSLKVIIDELDSISD-LPEKLSK 129

Query: 127 KTADNKTILALKNCHELLDLALDHLNISLSSSDITLLK------AVDDLKTWITSAATYQ 186
           +T D +   AL+ C +L++ ALD LN ++S+ D    K       ++DLKTW+++  T  
Sbjct: 130 ETEDERIKSALRVCGDLIEDALDRLNDTVSAIDDEEKKKTLSSSKIEDLKTWLSATVTDH 189

Query: 187 QTCIDDLAEV--------DPALADLVANFLKNSTELTSNGLAIVS-FFSKLTD-SLSLRR 246
           +TC D L E+        +  +   + + +  STE TSN LAIVS   S L+D  + + R
Sbjct: 190 ETCFDSLDELKQNKTEYANSTITQNLKSAMSRSTEFTSNSLAIVSKILSALSDLGIPIHR 249

Query: 247 LMSYENHQSNGD-----WARPILRKLAQSDIRKHADIVVAKDRSGKYKTITEALGAVPDK 306
                            WAR   R+L Q+   K  D+ VA D +G   T+ EA+  VP K
Sbjct: 250 XXXXXXXXXXXSVDFEKWAR---RRLLQTAGLK-PDVTVAGDGTGDVLTVNEAVAKVPKK 309

Query: 307 SKKRTVIYVKKGIYKENVEVVKNKWNVVMVGDGMTATIVSGNLNVVDGTPTFSTATFAAK 366
           S K  VIYVK G Y ENV + K+KWNV++ GDG   TI+SG+ N VDGTPT+ TATFA +
Sbjct: 310 SLKMFVIYVKSGTYVENVVMDKSKWNVMIYGDGKGKTIISGSKNFVDGTPTYETATFAIQ 369

Query: 367 GKGFIAIDMGFINTAGPSKHQAVALMSTSDQSIFYRCEMNAYQDTLYAHSNRQFYRECKV 426
           GKGFI  D+G INTAG +KHQAVA  S SD S++Y+C  + +QDTLY HSNRQFYR+C V
Sbjct: 370 GKGFIMKDIGIINTAGAAKHQAVAFRSGSDFSVYYQCSFDGFQDTLYPHSNRQFYRDCDV 429

Query: 427 YGTVDFIFGNSAVVLQNCTIAPRLPLLGQKNTITAQGRFDPNQNTGISIQGSLIQPFNDL 486
            GT+DFIFG++AVV Q C I PR PL  Q NTITAQG+ DPNQ++G+SIQ   I   N  
Sbjct: 430 TGTIDFIFGSAAVVFQGCKIMPRQPLSNQFNTITAQGKKDPNQSSGMSIQRCTISA-NGN 489

Query: 487 KTTETYLGRPWKNYSTTVFMQSNLGRLIHPTGWLPWI-GTSAPDTIFYAEFQNYGPGSST 546
               TYLGRPWK +STTV M++ +G ++ P+GW+ W+ G   P +I Y E++N GPGS  
Sbjct: 490 VIAPTYLGRPWKEFSTTVIMETVIGAVVRPSGWMSWVSGVDPPASIVYGEYKNTGPGSDV 549

Query: 547 SKRVKWKGVKNI-DTKTAKKFTVSSFIDGKDWI 557
           ++RVKW G K +     A KFTV++ + G DWI
Sbjct: 550 TQRVKWAGYKPVMSDAEAAKFTVATLLHGADWI 576

BLAST of CsaV3_1G037280 vs. Swiss-Prot
Match: sp|Q9LUL8|PME26_ARATH (Putative pectinesterase/pectinesterase inhibitor 26 OS=Arabidopsis thaliana OX=3702 GN=PME26 PE=2 SV=1)

HSP 1 Score: 396.0 bits (1016), Expect = 7.2e-109
Identity = 237/513 (46.20%), Postives = 310/513 (60.43%), Query Frame = 0

Query: 67  STSIKAVCDVTLYPDTCQKAFGPI-ANSSHLDPGQISKLSVQLALGELSKVADYIFDHAI 126
           S+ ++ VC+VT YP +C  +   +  + +  DP  + +LS+Q+   EL+ +   +     
Sbjct: 456 SSVLRTVCNVTNYPASCISSISKLPLSKTTTDPKVLFRLSLQVTFDELNSIVG-LPKKLA 515

Query: 127 TKTADNKTILALKNCHELLDLALDHLNISLSSSDITL--------LKAVDDLKTWITSAA 186
            +T D     AL  C ++ DLA+D +N ++SS D  +           + DL TW++SA 
Sbjct: 516 EETNDEGLKSALSVCADVFDLAVDSVNDTISSLDEVISGGKKNLNSSTIGDLITWLSSAV 575

Query: 187 TYQQTCIDDLAE--VDPALADLVANFLKNSTELTSNGLAIVSFF----SKLTDSLSLRRL 246
           T   TC D L E   +  +   + + + NSTE TSN LAIV+      SK    +  RRL
Sbjct: 576 TDIGTCGDTLDEDNYNSPIPQKLKSAMVNSTEFTSNSLAIVAQVLKKPSKSRIPVQGRRL 635

Query: 247 MSYENHQSNGDWARPILRKLAQS-DIRKHADIVVAKDRSGKYKTITEALGAVPDKSKKRT 306
           +   N  S  +W RP +R+L Q+ ++  H  + VA D SG  +T+ EA+  VP K K   
Sbjct: 636 L---NSNSFPNWVRPGVRRLLQAKNLTPH--VTVAADGSGDVRTVNEAVWRVPKKGKTMF 695

Query: 307 VIYVKKGIYKENVEVVKNKWNVVMVGDGMTATIVSGNLNVVDGTPTFSTATFAAKGKGFI 366
           VIYVK G Y ENV + K+KWNV + GDG   TI+SG+ N+VDG  TF+T+TFA +GKGF+
Sbjct: 696 VIYVKAGTYVENVLMKKDKWNVFIYGDGRDKTIISGSTNMVDGVRTFNTSTFATEGKGFM 755

Query: 367 AIDMGFINTAGPSKHQAVALMSTSDQSIFYRCEMNAYQDTLYAHSNRQFYRECKVYGTVD 426
             DMG INTAGP KHQAVA  S SD+S++YRC  + YQDTLY HSNRQ+YR C V GTVD
Sbjct: 756 MKDMGIINTAGPEKHQAVAFRSDSDRSVYYRCSFDGYQDTLYTHSNRQYYRNCDVTGTVD 815

Query: 427 FIFGNSAVVLQNCTIAPRLPLLGQKNTITAQGRFDPNQNTGISIQGSLIQPFNDLKTTET 486
           FIFG   VV Q C+I PR PL  Q NTITA+G  + NQNTGISI    I P N   T  T
Sbjct: 816 FIFGAGTVVFQGCSIRPRQPLPNQFNTITAEGTQEANQNTGISIHQCTISP-NGNVTATT 875

Query: 487 YLGRPWKNYSTTVFMQSNLGRLIHPTGWLPWIGT--SAPDTIFYAEFQNYGPGSSTSKRV 546
           YLGRPWK +S TV MQS +G  ++P GW+ W  T    P TIFY E++N GPGS  SKRV
Sbjct: 876 YLGRPWKLFSKTVIMQSVIGSFVNPAGWIAWNSTYDPPPRTIFYREYKNSGPGSDLSKRV 935

Query: 547 KWKGVKNIDT-KTAKKFTVSSFIDGKD-WISKA 560
           KW G K I +   A +FTV  F+ G D WI KA
Sbjct: 936 KWAGYKPISSDDEAARFTVKYFLRGDDNWIPKA 961

BLAST of CsaV3_1G037280 vs. TrEMBL
Match: tr|A0A0A0LY08|A0A0A0LY08_CUCSA (Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_1G538170 PE=3 SV=1)

HSP 1 Score: 1026.5 bits (2653), Expect = 2.2e-296
Identity = 567/567 (100.00%), Postives = 567/567 (100.00%), Query Frame = 0

Query: 1   MSFLRPYGKVDETDQLRLDARXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 60
           MSFLRPYGKVDETDQLRLDARXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX
Sbjct: 1   MSFLRPYGKVDETDQLRLDARXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 60

Query: 61  XXXXSLSTSIKAVCDVTLYPDTCQKAFGPIANSSHLDPGQISKLSVQLALGELSKVADYI 120
           XXXXSLSTSIKAVCDVTLYPDTCQKAFGPIANSSHLDPGQISKLSVQLALGELSKVADYI
Sbjct: 61  XXXXSLSTSIKAVCDVTLYPDTCQKAFGPIANSSHLDPGQISKLSVQLALGELSKVADYI 120

Query: 121 FDHAITKTADNKTILALKNCHELLDLALDHLNISLSSSDITLLKAVDDLKTWITSAATYQ 180
           FDHAITKTADNKTILALKNCHELLDLALDHLNISLSSSDITLLKAVDDLKTWITSAATYQ
Sbjct: 121 FDHAITKTADNKTILALKNCHELLDLALDHLNISLSSSDITLLKAVDDLKTWITSAATYQ 180

Query: 181 QTCIDDLAEVDPALADLVANFLKNSTELTSNGLAIVSFFSKLTDSLSLRRLMSYENHQSN 240
           QTCIDDLAEVDPALADLVANFLKNSTELTSNGLAIVSFFSKLTDSLSLRRLMSYENHQSN
Sbjct: 181 QTCIDDLAEVDPALADLVANFLKNSTELTSNGLAIVSFFSKLTDSLSLRRLMSYENHQSN 240

Query: 241 GDWARPILRKLAQSDIRKHADIVVAKDRSGKYKTITEALGAVPDKSKKRTVIYVKKGIYK 300
           GDWARPILRKLAQSDIRKHADIVVAKDRSGKYKTITEALGAVPDKSKKRTVIYVKKGIYK
Sbjct: 241 GDWARPILRKLAQSDIRKHADIVVAKDRSGKYKTITEALGAVPDKSKKRTVIYVKKGIYK 300

Query: 301 ENVEVVKNKWNVVMVGDGMTATIVSGNLNVVDGTPTFSTATFAAKGKGFIAIDMGFINTA 360
           ENVEVVKNKWNVVMVGDGMTATIVSGNLNVVDGTPTFSTATFAAKGKGFIAIDMGFINTA
Sbjct: 301 ENVEVVKNKWNVVMVGDGMTATIVSGNLNVVDGTPTFSTATFAAKGKGFIAIDMGFINTA 360

Query: 361 GPSKHQAVALMSTSDQSIFYRCEMNAYQDTLYAHSNRQFYRECKVYGTVDFIFGNSAVVL 420
           GPSKHQAVALMSTSDQSIFYRCEMNAYQDTLYAHSNRQFYRECKVYGTVDFIFGNSAVVL
Sbjct: 361 GPSKHQAVALMSTSDQSIFYRCEMNAYQDTLYAHSNRQFYRECKVYGTVDFIFGNSAVVL 420

Query: 421 QNCTIAPRLPLLGQKNTITAQGRFDPNQNTGISIQGSLIQPFNDLKTTETYLGRPWKNYS 480
           QNCTIAPRLPLLGQKNTITAQGRFDPNQNTGISIQGSLIQPFNDLKTTETYLGRPWKNYS
Sbjct: 421 QNCTIAPRLPLLGQKNTITAQGRFDPNQNTGISIQGSLIQPFNDLKTTETYLGRPWKNYS 480

Query: 481 TTVFMQSNLGRLIHPTGWLPWIGTSAPDTIFYAEFQNYGPGSSTSKRVKWKGVKNIDTKT 540
           TTVFMQSNLGRLIHPTGWLPWIGTSAPDTIFYAEFQNYGPGSSTSKRVKWKGVKNIDTKT
Sbjct: 481 TTVFMQSNLGRLIHPTGWLPWIGTSAPDTIFYAEFQNYGPGSSTSKRVKWKGVKNIDTKT 540

Query: 541 AKKFTVSSFIDGKDWISKAQVPFTATL 568
           AKKFTVSSFIDGKDWISKAQVPFTATL
Sbjct: 541 AKKFTVSSFIDGKDWISKAQVPFTATL 567

BLAST of CsaV3_1G037280 vs. TrEMBL
Match: tr|A0A1S3BDA4|A0A1S3BDA4_CUCME (Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103488419 PE=3 SV=1)

HSP 1 Score: 967.2 bits (2499), Expect = 1.6e-278
Identity = 536/567 (94.53%), Postives = 550/567 (97.00%), Query Frame = 0

Query: 1   MSFLRPYGKVDETDQLRLDARXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 60
           MSFLRPYGKVDETDQLRLDA XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX
Sbjct: 1   MSFLRPYGKVDETDQLRLDASXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 60

Query: 61  XXXXSLSTSIKAVCDVTLYPDTCQKAFGPIANSSHLDPGQISKLSVQLALGELSKVADYI 120
           XXXXSLS SIKAVCDVTLYPDTCQKAFGP ANSSHLDPGQISKL+VQLALGELSKVAD+I
Sbjct: 61  XXXXSLSASIKAVCDVTLYPDTCQKAFGPFANSSHLDPGQISKLAVQLALGELSKVADHI 120

Query: 121 FDHAITKTADNKTILALKNCHELLDLALDHLNISLSSSDITLLKAVDDLKTWITSAATYQ 180
           FDHAIT T D+KTILALKNCHELLDLALDHLNI+LSSSDITLLKAVDDLKTW+TSAATYQ
Sbjct: 121 FDHAITNTTDDKTILALKNCHELLDLALDHLNITLSSSDITLLKAVDDLKTWLTSAATYQ 180

Query: 181 QTCIDDLAEVDPALADLVANFLKNSTELTSNGLAIVSFFSKLTDSLSLRRLMSYENHQSN 240
           QTCID LAEVDPALADL ANFLKNSTELTSNGLAIVSFFSKLTDSLSLRRLMSYE+ QSN
Sbjct: 181 QTCIDGLAEVDPALADLFANFLKNSTELTSNGLAIVSFFSKLTDSLSLRRLMSYEDRQSN 240

Query: 241 GDWARPILRKLAQSDIRKHADIVVAKDRSGKYKTITEALGAVPDKSKKRTVIYVKKGIYK 300
           GDWARPILRKLAQSDIRK ADIVVAKD SGKYKTITEAL AVPDKSKKRTVIYVKKG YK
Sbjct: 241 GDWARPILRKLAQSDIRKQADIVVAKDSSGKYKTITEALKAVPDKSKKRTVIYVKKGTYK 300

Query: 301 ENVEVVKNKWNVVMVGDGMTATIVSGNLNVVDGTPTFSTATFAAKGKGFIAIDMGFINTA 360
           E VEVVKNKWNVVMVGDGM ATIVSG+LNV+DGTPTFSTA+FAAKGKGFIAIDMGFINTA
Sbjct: 301 EKVEVVKNKWNVVMVGDGMNATIVSGSLNVIDGTPTFSTASFAAKGKGFIAIDMGFINTA 360

Query: 361 GPSKHQAVALMSTSDQSIFYRCEMNAYQDTLYAHSNRQFYRECKVYGTVDFIFGNSAVVL 420
           GPSKHQAVALMSTSDQSIFYRC+MNAYQDTLYAHSNRQFYRECKVYGTVDFIFGNSAVVL
Sbjct: 361 GPSKHQAVALMSTSDQSIFYRCQMNAYQDTLYAHSNRQFYRECKVYGTVDFIFGNSAVVL 420

Query: 421 QNCTIAPRLPLLGQKNTITAQGRFDPNQNTGISIQGSLIQPFNDLKTTETYLGRPWKNYS 480
           QNCTIAPRLPLLGQKNTITAQGRFDPNQNTGISIQGSLIQPF++LKTTETYLGRPWKNYS
Sbjct: 421 QNCTIAPRLPLLGQKNTITAQGRFDPNQNTGISIQGSLIQPFDNLKTTETYLGRPWKNYS 480

Query: 481 TTVFMQSNLGRLIHPTGWLPWIGTSAPDTIFYAEFQNYGPGSSTSKRVKWKGVKNIDTKT 540
           TTVFMQSNLG LIHP+GWLPW+GTSAPDTIFYAEFQNYG GSSTSKRVKWKGVKNID KT
Sbjct: 481 TTVFMQSNLGSLIHPSGWLPWVGTSAPDTIFYAEFQNYGRGSSTSKRVKWKGVKNIDIKT 540

Query: 541 AKKFTVSSFIDGKDWISKAQVPFTATL 568
           AKKFTVSSFIDGKDWISKAQVPFTATL
Sbjct: 541 AKKFTVSSFIDGKDWISKAQVPFTATL 567

BLAST of CsaV3_1G037280 vs. TrEMBL
Match: tr|A0A2N9G0Y0|A0A2N9G0Y0_FAGSY (Pectinesterase OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS21045 PE=3 SV=1)

HSP 1 Score: 649.0 bits (1673), Expect = 9.5e-183
Identity = 330/569 (58.00%), Postives = 404/569 (71.00%), Query Frame = 0

Query: 1   MSFLRPYGKVDETDQLRLDARXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 60
           MS  R YGKVDE  Q +L+AR                                       
Sbjct: 4   MSSFRAYGKVDEVQQSKLEARRKTRKRVTIISISSIILVCVIVAAVLGTSHRSSENSQSG 63

Query: 61  XXXXSLSTSIKAVCDVTLYPDTCQKAFGPIANSSHLDPGQISKLSVQLALGELSKVADYI 120
               SL+TSIKA+CD+TLYP+TC  +  P+ NS+ + P  I  L+ Q+AL EL K A Y 
Sbjct: 64  GNSQSLTTSIKAICDITLYPNTCYTSLAPLNNSTQIQPEGIFNLATQVALNELYKAAQYF 123

Query: 121 FDH-AITKTADNKTILALKNCHELLDLALDHLNISLSSSDITLLKAVDDLKTWITSAATY 180
            +H  +  + DN T+ AL+NCH++L LALDHLN++LSSS ++LL+A DD KTW++SA TY
Sbjct: 124 SEHNVLNGSTDNMTVSALENCHQVLSLALDHLNMTLSSSSLSLLEAADDFKTWLSSAGTY 183

Query: 181 QQTCIDDLAEVDPALADLVANFLKNSTELTSNGLAIVSFFSKLTDSLSLRRLMSYENHQS 240
           Q+TC +        L   + ++L+ STELTSN LAI+S+ + +  SL LRRLMS+  H  
Sbjct: 184 QETCKEGFENASATLKSSIDDWLQLSTELTSNSLAIISWIANVEGSLKLRRLMSFPGHYE 243

Query: 241 NGDWARPILRKLAQS-DIRKHADIVVAKDRSGKYKTITEALGAVPDKSKKRTVIYVKKGI 300
           + +W  P  RKL QS D+RK A+I+VA D SGKYK I+ AL AVP+KS  R VIYVKKGI
Sbjct: 244 DPNWLNPKDRKLLQSPDLRKQANIIVALDGSGKYKNISAALNAVPNKSSNRFVIYVKKGI 303

Query: 301 YKENVEVVKNKWNVVMVGDGMTATIVSGNLNVVDGTPTFSTATFAAKGKGFIAIDMGFIN 360
           Y ENV+V KNKWN+VMVGDGM  ++VSG+LN VDGTPTFSTATFA  GKGFIAIDMGF N
Sbjct: 304 YYENVQVNKNKWNIVMVGDGMNDSVVSGSLNFVDGTPTFSTATFAVFGKGFIAIDMGFQN 363

Query: 361 TAGPSKHQAVALMSTSDQSIFYRCEMNAYQDTLYAHSNRQFYRECKVYGTVDFIFGNSAV 420
           TAG  KHQAVAL+S SDQSIFYRC ++A+QDTLYAH+NRQFYREC + GTVDFIFGNSAV
Sbjct: 364 TAGAIKHQAVALLSDSDQSIFYRCFVDAFQDTLYAHTNRQFYRECNITGTVDFIFGNSAV 423

Query: 421 VLQNCTIAPRLPLLGQKNTITAQGRFDPNQNTGISIQGSLIQPFNDLKTTETYLGRPWKN 480
           V Q+C I PR+PLLGQ+ TITAQG+ DPNQNTGISIQ   I PF +L + +TYLGRPWKN
Sbjct: 424 VFQDCNILPRVPLLGQQITITAQGKIDPNQNTGISIQNCTIFPFGNLSSIQTYLGRPWKN 483

Query: 481 YSTTVFMQSNLGRLIHPTGWLPWIGTSAPDTIFYAEFQNYGPGSSTSKRVKWKGVKNIDT 540
           YSTTV++ +++G  I   GWLPW+GTSAPDTIFY+EFQNYGPGSST  RV+WKGVKNI +
Sbjct: 484 YSTTVYIGNSMGSFIDSNGWLPWVGTSAPDTIFYSEFQNYGPGSSTKDRVQWKGVKNITS 543

Query: 541 KTAKKFTVSSFIDGKDWISKAQVPFTATL 568
             A KFTV SFI G  WI  A VP+ ++L
Sbjct: 544 HQASKFTVKSFIKGDKWIPAAGVPYKSSL 572

BLAST of CsaV3_1G037280 vs. TrEMBL
Match: tr|A0A2P6SBF6|A0A2P6SBF6_ROSCH (Pectinesterase OS=Rosa chinensis OX=74649 GN=RchiOBHm_Chr1g0331001 PE=3 SV=1)

HSP 1 Score: 645.2 bits (1663), Expect = 1.4e-181
Identity = 329/573 (57.42%), Postives = 415/573 (72.43%), Query Frame = 0

Query: 1   MSFLRPYGKVDETDQLRLDARXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 60
           MS  + YGKV E+DQ +LDAR                                       
Sbjct: 1   MSSFKAYGKVSESDQAKLDAR-RKTCRRVAIISISSIVLVCVVVAAVVGTATHNSKKGDD 60

Query: 61  XXXXSLSTSIKAVCDVTLYPDTCQKAFGPIANSSHLDPGQISKLSVQLALGELSKVADYI 120
               +LS S+KAVCDVTLY D+C  + GPI NSS+  P  + KLS+Q+A+ ELSK A  I
Sbjct: 61  NGKGTLSASVKAVCDVTLYQDSCYSSLGPIVNSSNFKPEDLFKLSIQVAITELSKAATQI 120

Query: 121 FDHAITK--TADNKTILALKNCHELLDLALDHLNISLSSSDITLLKAVDDLKTWITSAAT 180
            D  + K    D  ++ ALKNC ELL LALDHLN SL S D +L++ V+DLKTW++SA T
Sbjct: 121 SDQGLFKGTVTDKLSVAALKNCGELLSLALDHLNESL-SGDGSLVEVVEDLKTWLSSAGT 180

Query: 181 YQQTCIDDLAEVDPALADLVANFLKNSTELTSNGLAIVSFFSKLTDSLSLRRLMSYENHQ 240
           +QQ+C+D   +    L   +  +LK STELTSN LAIVS+ SK+ +S+ LRRL+S++   
Sbjct: 181 FQQSCVDGFEDAKGDLKATIEKYLKTSTELTSNSLAIVSWISKVANSVKLRRLLSHDGAA 240

Query: 241 SN---GDWARPILRKLAQ-SDIRKHADIVVAKDRSGKYKTITEALGAVPDKSKKRTVIYV 300
           ++    +W     R+L + SD+RK A+IVVAKD SGKYKT+  AL AVPDKSKKRTVIYV
Sbjct: 241 ASHGMPEWLHQTDRRLIESSDLRKKANIVVAKDGSGKYKTVGAALKAVPDKSKKRTVIYV 300

Query: 301 KKGIYKENVEVVKNKWNVVMVGDGMTATIVSGNLNVVDGTPTFSTATFAAKGKGFIAIDM 360
           KKG+Y ENV V K+KWNVV+VGDGMT+TIVS +LNVVDGTPTFS+ATFA  GKGF+A D+
Sbjct: 301 KKGVYFENVRVEKSKWNVVVVGDGMTSTIVSASLNVVDGTPTFSSATFAVFGKGFVARDI 360

Query: 361 GFINTAGPSKHQAVALMSTSDQSIFYRCEMNAYQDTLYAHSNRQFYRECKVYGTVDFIFG 420
           G  NTAG +KHQAVALMS++DQ++FYRC ++A+QD+LY H+NRQFYREC +YGTVDFIFG
Sbjct: 361 GIRNTAGAAKHQAVALMSSADQTVFYRCHIDAFQDSLYVHANRQFYRECNIYGTVDFIFG 420

Query: 421 NSAVVLQNCTIAPRLPLLGQKNTITAQGRFDPNQNTGISIQGSLIQPFNDLKTTETYLGR 480
           NSAVVLQNC + P++P++GQ+NTITAQG+ DPNQNTGI+ Q   I PF DL + +TYLGR
Sbjct: 421 NSAVVLQNCKVFPKVPMVGQQNTITAQGKIDPNQNTGIAFQNCTISPFGDLSSVKTYLGR 480

Query: 481 PWKNYSTTVFMQSNLGRLIHPTGWLPWIGTSAPDTIFYAEFQNYGPGSSTSKRVKWKGVK 540
           PWKNYSTTV+MQ+ +G LIHP GWLPW+GT+APD+IFY+EFQN+GPGS T  RVKWKG+K
Sbjct: 481 PWKNYSTTVYMQTFMGSLIHPDGWLPWVGTTAPDSIFYSEFQNFGPGSVTKNRVKWKGLK 540

Query: 541 NIDTKTAKKFTVSSFIDGKDWISKAQVPFTATL 568
            I +K A KFTV SF++G +WIS A VP+ + L
Sbjct: 541 TITSKVAGKFTVHSFLEGTNWISDASVPYKSNL 571

BLAST of CsaV3_1G037280 vs. TrEMBL
Match: tr|A0A2P4HGI0|A0A2P4HGI0_QUESU (Pectinesterase OS=Quercus suber OX=58331 GN=CFP56_61652 PE=3 SV=1)

HSP 1 Score: 638.3 bits (1645), Expect = 1.7e-179
Identity = 329/566 (58.13%), Postives = 397/566 (70.14%), Query Frame = 0

Query: 1   MSFLRPYGKVDETDQLRLDARXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 60
           MS  + YGK+DE  QL+ +AR                                       
Sbjct: 1   MSSFKAYGKIDEAQQLKHEARQKTRKRVTIISISSIILACVIVGAVLGTSHSSRENSQSG 60

Query: 61  XXXXSLSTSIKAVCDVTLYPDTCQKAFGPIANSSHLDPGQISKLSVQLALGELSKVADYI 120
               S + SIKAVCD+TLYP+TC  +  P+ NSSH+ P  I K + Q+A+ ELSKVA Y 
Sbjct: 61  GTSQSSAASIKAVCDLTLYPNTCYSSLSPLNNSSHIQPEDIFKFATQVAMNELSKVAQYF 120

Query: 121 FDH-AITKTADNKTILALKNCHELLDLALDHLNISLSSSDITLLKAVDDLKTWITSAATY 180
            DH A+  +ADN T+ AL+NC +LL LALDHLN+ LSS  +TLL+AVDDLKTW++SA TY
Sbjct: 121 SDHNALNGSADNTTVAALENCVQLLSLALDHLNMVLSSGSLTLLEAVDDLKTWLSSAGTY 180

Query: 181 QQTCIDDLAEVDPALADLVANFLKNSTELTSNGLAIVSFFSKLTDSLSLRRLMSYENHQS 240
           Q TC +        +   + N+L+NSTELTSN LAI+S+ S +  SL LRRLMS+  H  
Sbjct: 181 QDTCKEGFETASATVKTSITNWLQNSTELTSNCLAIISWISNVESSLKLRRLMSFTGHYE 240

Query: 241 NGDWARPILRKLAQS-DIRKHADIVVAKDRSGKYKTITEALGAVPDKSKKRTVIYVKKGI 300
           +  W  P  RKL QS D++K A+I+VA+D SG+YK I+ AL AVPDKS  R VIYVKKG 
Sbjct: 241 HPKWLNPKDRKLLQSWDVKKQANIIVAQDGSGQYKNISAALTAVPDKSTNRFVIYVKKGS 300

Query: 301 YKENVEVVKNKWNVVMVGDGMTATIVSGNLNVVDGTPTFSTATFAAKGKGFIAIDMGFIN 360
           Y E V+V KNKWN+VMVGDGM  T+V G LN +DGTPTFSTATFA  GKGFIAIDMGF N
Sbjct: 301 YYEKVKVNKNKWNIVMVGDGMNDTVVYGGLNNIDGTPTFSTATFAVTGKGFIAIDMGFRN 360

Query: 361 TAGPSKHQAVALMSTSDQSIFYRCEMNAYQDTLYAHSNRQFYRECKVYGTVDFIFGNSAV 420
            AG  KHQAVAL+S SD SIFYRC ++A+QD+LYAH+NRQFY EC + GTVDFIFGNSAV
Sbjct: 361 IAGAIKHQAVALLSDSDHSIFYRCFIDAFQDSLYAHTNRQFYSECNITGTVDFIFGNSAV 420

Query: 421 VLQNCTIAPRLPLLGQKNTITAQGRFDPNQNTGISIQGSLIQPFNDLK-TTETYLGRPWK 480
             QNC I P+ PLLGQ+NTITAQG+FDPNQNTGISIQ   I P+     + +TYLGRPWK
Sbjct: 421 AFQNCNILPKEPLLGQQNTITAQGKFDPNQNTGISIQNCTIFPYGYANLSCQTYLGRPWK 480

Query: 481 NYSTTVFMQSNLGRLIHPTGWLPWIGTSAPDTIFYAEFQNYGPGSSTSKRVKWKGVKNID 540
           NYSTTV++ ++LG LI  +GWLPW+GTSAPDTIFY+EFQNYGPGSST  RV+WKGVKN+ 
Sbjct: 481 NYSTTVYIGNSLGSLIDSSGWLPWVGTSAPDTIFYSEFQNYGPGSSTKDRVQWKGVKNMT 540

Query: 541 TKTAKKFTVSSFIDGKDWISKAQVPF 564
           +  A KFTVSSFI G  WI  A VP+
Sbjct: 541 SHQASKFTVSSFIKGDKWIPAAGVPY 566