Cp4.1LG20g02050 (gene) Cucurbita pepo (Zucchini)

Overview
NameCp4.1LG20g02050
Typegene
OrganismCucurbita pepo (Cucurbita pepo (Zucchini))
Description2-oxoglutarate-dependent dioxygenase
LocationCp4.1LG20 : 1104985 .. 1106131 (-)
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCGGGTGAGATTCCAGTTGTGAGATTTAGTTCACACAATTTGCAGAGCGGAGGGAGGAAGTGGGGTTCGACGAGGGAGAAGGTCCGGGAAGCACTTGAAGAGTATGGATGCTTTGTAGCGTTGTACGACAGCGTTTCAATGGAGGCGAGTTCTTTGAAGATGATGGAAGCCATGAAGGAGGTGTTCGAAGTTCCATTGGAGAGGAAGGTTCAGAATGTGTCTGAGAAGCCTTTCCATGGCTACTTTGGGCAGAACCCATTGATGCCTATCCATGAAAGTATGGGCATTGAAGACCCTCTTCTTCCCTCCAACATCCAATCTTTCTCCAACCTCATGTGGCCCTCTCGCCCAAACCCTCTGTTCTGGTAAATTTCTTCCCTTCGTTCATTCTGCCTTTATTTCTCACTTTCAGAGCTAACCCACATACTATTTTGCAGTGAAAACCTCAAATCCTACGCAGAATTGGTGTCCCAATTGGAGCAGACGGTGAAGAAGATGGTGTTCGAGAGCTACGGCCTCAAAAGCCTCTTCCTTTCACACACCCAATCCACTAAATATCTTCTCCGGATGATCAAATACAGAGTCCCACATCGGAACGAGATGAATTTGGGGGCTTTCCCTCACACCGACAAGAGCTTCCTCACCATTCTTCATCAGAATTCAGTAAATGGGTTACACATTAAGACCAGAGACAACAGATGGGTTGAATATCAGCCTCCTTCTGCTGAATCTTCTGCCAAATCATTCATCGTCATGGCTGGAGATGCCTTCTTTGTAAGTTGCCATTAACTTCAAATTCCATTACAATTAGCAATAACCCATCAAAAACTTACAAAGAAATGTGGGGTTGTTGGGACAGGCGTGGAGCAATGGGAGGATATATTCGGCGCCACACAGAGTGACGATGAGTGGAAACAGAGAGAGATACTCAGTGGGGCTGTTTTCGTTCAACGATGGGATTGTTCAGATTCCAAAAGAATTGGTGGACGACAAACATCCGCAACAGTTTAAGGCGTTTGATCATCAAGATTACCTTCGTTTCTACAGCACTGAGAAAGGCCAAAGCTCCCCTTCTGCTATTAAAGCTTATTGTGGCGTTGCAAATGGCCTCCTTGATTCACGAACTTGCATTCCCATGCATTAA

mRNA sequence

ATGGCGGGTGAGATTCCAGTTGTGAGATTTAGTTCACACAATTTGCAGAGCGGAGGGAGGAAGTGGGGTTCGACGAGGGAGAAGGTCCGGGAAGCACTTGAAGAGTATGGATGCTTTGTAGCGTTGTACGACAGCGTTTCAATGGAGGCGAGTTCTTTGAAGATGATGGAAGCCATGAAGGAGGTGTTCGAAGTTCCATTGGAGAGGAAGGTTCAGAATGTGTCTGAGAAGCCTTTCCATGGCTACTTTGGGCAGAACCCATTGATGCCTATCCATGAAAGTATGGGCATTGAAGACCCTCTTCTTCCCTCCAACATCCAATCTTTCTCCAACCTCATGTGGCCCTCTCGCCCAAACCCTCTGTTCTGTGAAAACCTCAAATCCTACGCAGAATTGGTGTCCCAATTGGAGCAGACGGTGAAGAAGATGGTGTTCGAGAGCTACGGCCTCAAAAGCCTCTTCCTTTCACACACCCAATCCACTAAATATCTTCTCCGGATGATCAAATACAGAGTCCCACATCGGAACGAGATGAATTTGGGGGCTTTCCCTCACACCGACAAGAGCTTCCTCACCATTCTTCATCAGAATTCAGTAAATGGGTTACACATTAAGACCAGAGACAACAGATGGGTTGAATATCAGCCTCCTTCTGCTGAATCTTCTGCCAAATCATTCATCGTCATGGCTGGAGATGCCTTCTTTGCGTGGAGCAATGGGAGGATATATTCGGCGCCACACAGAGTGACGATGAGTGGAAACAGAGAGAGATACTCAGTGGGGCTGTTTTCGTTCAACGATGGGATTGTTCAGATTCCAAAAGAATTGGTGGACGACAAACATCCGCAACAGTTTAAGGCGTTTGATCATCAAGATTACCTTCGTTTCTACAGCACTGAGAAAGGCCAAAGCTCCCCTTCTGCTATTAAAGCTTATTGTGGCGTTGCAAATGGCCTCCTTGATTCACGAACTTGCATTCCCATGCATTAA

Coding sequence (CDS)

ATGGCGGGTGAGATTCCAGTTGTGAGATTTAGTTCACACAATTTGCAGAGCGGAGGGAGGAAGTGGGGTTCGACGAGGGAGAAGGTCCGGGAAGCACTTGAAGAGTATGGATGCTTTGTAGCGTTGTACGACAGCGTTTCAATGGAGGCGAGTTCTTTGAAGATGATGGAAGCCATGAAGGAGGTGTTCGAAGTTCCATTGGAGAGGAAGGTTCAGAATGTGTCTGAGAAGCCTTTCCATGGCTACTTTGGGCAGAACCCATTGATGCCTATCCATGAAAGTATGGGCATTGAAGACCCTCTTCTTCCCTCCAACATCCAATCTTTCTCCAACCTCATGTGGCCCTCTCGCCCAAACCCTCTGTTCTGTGAAAACCTCAAATCCTACGCAGAATTGGTGTCCCAATTGGAGCAGACGGTGAAGAAGATGGTGTTCGAGAGCTACGGCCTCAAAAGCCTCTTCCTTTCACACACCCAATCCACTAAATATCTTCTCCGGATGATCAAATACAGAGTCCCACATCGGAACGAGATGAATTTGGGGGCTTTCCCTCACACCGACAAGAGCTTCCTCACCATTCTTCATCAGAATTCAGTAAATGGGTTACACATTAAGACCAGAGACAACAGATGGGTTGAATATCAGCCTCCTTCTGCTGAATCTTCTGCCAAATCATTCATCGTCATGGCTGGAGATGCCTTCTTTGCGTGGAGCAATGGGAGGATATATTCGGCGCCACACAGAGTGACGATGAGTGGAAACAGAGAGAGATACTCAGTGGGGCTGTTTTCGTTCAACGATGGGATTGTTCAGATTCCAAAAGAATTGGTGGACGACAAACATCCGCAACAGTTTAAGGCGTTTGATCATCAAGATTACCTTCGTTTCTACAGCACTGAGAAAGGCCAAAGCTCCCCTTCTGCTATTAAAGCTTATTGTGGCGTTGCAAATGGCCTCCTTGATTCACGAACTTGCATTCCCATGCATTAA

Protein sequence

MAGEIPVVRFSSHNLQSGGRKWGSTREKVREALEEYGCFVALYDSVSMEASSLKMMEAMKEVFEVPLERKVQNVSEKPFHGYFGQNPLMPIHESMGIEDPLLPSNIQSFSNLMWPSRPNPLFCENLKSYAELVSQLEQTVKKMVFESYGLKSLFLSHTQSTKYLLRMIKYRVPHRNEMNLGAFPHTDKSFLTILHQNSVNGLHIKTRDNRWVEYQPPSAESSAKSFIVMAGDAFFAWSNGRIYSAPHRVTMSGNRERYSVGLFSFNDGIVQIPKELVDDKHPQQFKAFDHQDYLRFYSTEKGQSSPSAIKAYCGVANGLLDSRTCIPMH
Homology
[Close]
BLAST of Cp4.1LG20g02050 vs. Swiss-Prot
Match: AOP1L_ARATH (Probable 2-oxoglutarate-dependent dioxygenase AOP1.2 OS=Arabidopsis thaliana GN=AOP1.2 PE=2 SV=1)

HSP 1 Score: 254.2 bits (648), Expect = 1.9e-66
Identity = 127/316 (40.19%), Postives = 196/316 (62.03%), Query Frame = 1 

Query: 4   EIPVVRFSSHNLQSGGRKWGSTREKVREALEEYGCFVALYDSVSMEASSLKMMEAMKEVF 63
           ++PV+ FS  NL+ G  KW   +  V +ALE+YGCF A +D +S+E +   + EAM+++F
Sbjct: 13  QLPVIDFSDQNLKPGSSKWDEVKADVLKALEDYGCFEAFFDKLSVELNR-SVFEAMEDLF 72

Query: 64  EVPLERKVQNVSEKPFHGYFGQNPLMPIHESMGIEDPLLPSNIQSFSNLMWPSRPNPLFC 123
           E+P+  K +NVS KPFHGY   N    ++ES+GI+D  +   +  F+  +WP   N    
Sbjct: 73  ELPIPTKQRNVSSKPFHGYLCHN----LYESLGIDDANVLEKVNDFTQQLWPDHGNKSIS 132

Query: 124 ENLKSYAELVSQLEQTVKKMVFESYGLKSLFLSHTQSTKYLLRMIKYRVP-----HRNEM 183
           E +  ++E + +L+  V++M+ ES+G+++    H  ST YL R++KY  P        E 
Sbjct: 133 ETIHLFSEQLVELDLMVRRMIMESFGIENYIDEHLNSTYYLTRLMKYTSPPDDDDDDEET 192

Query: 184 NLGAFPHTDKSFLTILHQNSVNGLHIKTRDNRWVEYQPPSAESSAKSFIVMAGDAFFAWS 243
            LG   HTDK+ +TILHQ  V+GL +KT+D++W++ +P     S  S +VM GD+  A  
Sbjct: 193 KLGLRSHTDKNIITILHQYQVDGLEVKTKDDKWIKVKP-----SQDSVLVMVGDSLCALL 252

Query: 244 NGRIYSAPHRVTMSGNRERYSVGLFSF--NDGIVQIPKELVDDKHPQQFKAFDHQDYLRF 303
           NGR++S  HRV M+G + RYS GLFS      I+  P+ELVD +HP+ FK F++ D+L F
Sbjct: 253 NGRLHSPYHRVIMTGKKTRYSTGLFSIPKTGVIIDSPEELVDKEHPRIFKPFEYTDFLHF 312

Query: 304 YSTEKGQSSPSAIKAY 313
           + TE G+ + SA+ A+
Sbjct: 313 FQTEAGRIAQSALHAF 318

[Close]
BLAST of Cp4.1LG20g02050 vs. Swiss-Prot
Match: AOP1C_ARATH (Probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Arabidopsis thaliana GN=AOP1 PE=2 SV=1)

HSP 1 Score: 251.1 bits (640), Expect = 1.6e-65
Identity = 127/317 (40.06%), Postives = 195/317 (61.51%), Query Frame = 1 

Query: 4   EIPVVRFSSHNLQSGGRKWGSTREKVREALEEYGCFVALYDSVSMEASSLKMMEAMKEVF 63
           ++PV+ FS  NL+ G  KW      V +ALE+YGCF A +D +S+E +   + EAM+++F
Sbjct: 13  QLPVIDFSDQNLKPGSSKWDEVTADVLKALEDYGCFEASFDKLSVELNR-SVFEAMEDLF 72

Query: 64  EVPLERKVQNVSEKPFHGYFGQNPLMPIHESMGIEDPLLPSNIQSFSNLMWPSRPNPLFC 123
           E+P+  K +NVS KPFHGY   N    ++ES+GI D  +   +  F+  +WP   N    
Sbjct: 73  ELPIPTKQRNVSSKPFHGYLCHN----LYESLGINDANVLEKVNDFTQQLWPDHGNKSIS 132

Query: 124 ENLKSYAELVSQLEQTVKKMVFESYGLKSLFLSHTQSTKYLLRMIKYRVPHRN------E 183
           E +  ++E + +L+  V++M+ ES+G+++    H  ST YL R++KY  P  +      E
Sbjct: 133 ETIHLFSEQLVELDLMVRRMIMESFGIENYIDEHLNSTYYLTRLMKYTSPPDDDDDDDEE 192

Query: 184 MNLGAFPHTDKSFLTILHQNSVNGLHIKTRDNRWVEYQPPSAESSAKSFIVMAGDAFFAW 243
             LG   HTDK+ +TILHQ  V+GL +KT+D++W++ +P     S  S +VM GD+  A 
Sbjct: 193 TKLGLRSHTDKNIITILHQYQVDGLEVKTKDDKWIKVKP-----SQDSVLVMVGDSLCAL 252

Query: 244 SNGRIYSAPHRVTMSGNRERYSVGLFSF--NDGIVQIPKELVDDKHPQQFKAFDHQDYLR 303
            NGR++S  HRV M+G + RYS GLFS      I+  P+ELVD +HP+ FK F++ D+L 
Sbjct: 253 LNGRLHSPYHRVIMTGKKTRYSTGLFSIPKTGVIIDSPEELVDKEHPRIFKPFEYTDFLH 312

Query: 304 FYSTEKGQSSPSAIKAY 313
           F+ TE G+ + SA+ A+
Sbjct: 313 FFQTEAGRIAQSALHAF 319

[Close]
BLAST of Cp4.1LG20g02050 vs. Swiss-Prot
Match: AOP1V_ARATH (Probable 2-oxoglutarate-dependent dioxygenase AOP1 (Fragment) OS=Arabidopsis thaliana GN=AOP1 PE=2 SV=1)

HSP 1 Score: 241.9 bits (616), Expect = 9.9e-63
Identity = 123/306 (40.20%), Postives = 187/306 (61.11%), Query Frame = 1 

Query: 4   EIPVVRFSSHNLQSGGRKWGSTREKVREALEEYGCFVALYDSVSMEASSLKMMEAMKEVF 63
           ++PV+ FS  NL+ G  KW      V +ALE+YGCF A +D +S+E +   + EAM+++F
Sbjct: 6   QLPVIDFSDQNLKPGSSKWDEVTADVLKALEDYGCFEASFDKLSVELNR-SVFEAMEDLF 65

Query: 64  EVPLERKVQNVSEKPFHGYFGQNPLMPIHESMGIEDPLLPSNIQSFSNLMWPSRPNPLFC 123
           E+P+  K +NVS K FHGY   N    ++ES+GI D  +   +  F+  +WP   N    
Sbjct: 66  ELPIPTKQRNVSSKLFHGYLCHN----LYESLGINDANVLEKVNDFTQQLWPDHGNKSIS 125

Query: 124 ENLKSYAELVSQLEQTVKKMVFESYGLKSLFLSHTQSTKYLLRMIKYRVPHRN----EMN 183
           E +  ++E + +L+  V++M+ ES+G++     H  ST YL R++KY  P  +    E  
Sbjct: 126 ETIHLFSEQLVELDLMVRRMIMESFGIEKYIDEHLNSTYYLTRLMKYTSPPDDDDDEETK 185

Query: 184 LGAFPHTDKSFLTILHQNSVNGLHIKTRDNRWVEYQPPSAESSAKSFIVMAGDAFFAWSN 243
           LG   HTDK+ +TILHQ  V+GL +KT+D++W++ +P     S  S +VM GD+  A  N
Sbjct: 186 LGLRSHTDKNIITILHQYQVDGLEVKTKDDKWIKVKP-----SQDSVLVMVGDSLCALLN 245

Query: 244 GRIYSAPHRVTMSGNRERYSVGLFSF--NDGIVQIPKELVDDKHPQQFKAFDHQDYLRFY 303
           GR++S  HRV M+G + RYS GLFS      I+  P+ELVD +HP+ FK F++ D+L F+
Sbjct: 246 GRLHSPYHRVIMTGKKTRYSTGLFSIPKTGVIIDSPEELVDKEHPRIFKPFEYTDFLHFF 301

[Close]
BLAST of Cp4.1LG20g02050 vs. Swiss-Prot
Match: AOP2V_ARATH (2-oxoglutarate-dependent dioxygenase AOP2 OS=Arabidopsis thaliana GN=AOP2 PE=2 SV=1)

HSP 1 Score: 125.6 bits (314), Expect = 1.0e-27
Identity = 62/185 (33.51%), Postives = 106/185 (57.30%), Query Frame = 1 

Query: 4   EIPVVRFSSHNLQSGGRKWGSTREKVREALEEYGCFVALYDSVSMEASSLKMMEAMKEVF 63
           ++P++  +   L+ G  KW   R  VR+ALE++GCF A YD VS+E     +M+ M+E+F
Sbjct: 7   QLPLINLADKTLEPGSSKWAEVRSDVRKALEDFGCFEASYDKVSLELQE-SIMKTMEELF 66

Query: 64  EVPLERKVQNVSEKPFHGYFGQNPLMPIHESMGIEDPLLPSNIQSFSNLMWPSRP-NPLF 123
            +P+E K +NV  KP+ GY   N L    ES+GI +  +  N+  F+  +WP    N   
Sbjct: 67  ALPVETKQRNVCPKPYVGYLNHNNL---SESLGISNANILENVNEFTQQLWPDGDGNENI 126

Query: 124 CENLKSYAELVSQLEQTVKKMVFESYGLKSLFLSHTQSTKYLLRMIKYRVPHRNEMNLGA 183
            + ++ +AE + +++  V++MV ES+G++     H +ST+Y +R++KY  P   + N   
Sbjct: 127 SKTIQLFAEKLVEIDVMVRRMVMESFGIEKYIDDHLKSTEYRMRLMKYIAPPEGDANTTV 186

Query: 184 FPHTD 188
             + D
Sbjct: 187 DDYAD 187

[Close]
BLAST of Cp4.1LG20g02050 vs. Swiss-Prot
Match: AOP3L_ARATH (2-oxoglutarate-dependent dioxygenase AOP3 OS=Arabidopsis thaliana GN=AOP3 PE=2 SV=1)

HSP 1 Score: 124.4 bits (311), Expect = 2.3e-27
Identity = 63/145 (43.45%), Postives = 94/145 (64.83%), Query Frame = 1 

Query: 177 EMNLGAFPHTDKSFLTILHQNSVNGLHIKTRDNRWVEYQPPSAESSAKSFIVMAGDAFFA 236
           +  LG   HTDKS   I++Q+ ++GL +KT++ +W+  +P     +  + IV+AGDA  A
Sbjct: 270 DAELGLPSHTDKSLSGIIYQHQIDGLEVKTKEGKWIRVKP-----APNTVIVIAGDALCA 329

Query: 237 WSNGRIYSAPHRVTMSGNRE-RYSVGLFSF-NDG-IVQIPKELVDDKHPQQFKAFDHQDY 296
             NGRI S  HRV ++  ++ RY+  LFS+  +G I+  PKELVD+KHP+ FK FD  D 
Sbjct: 330 LMNGRIPSPYHRVRVTERKKTRYAAALFSYPKEGYIIDSPKELVDEKHPRAFKPFDFVDL 389

Query: 297 LRFYSTEKGQSSPSAIKAYCGVANG 319
             FY TE G+ +PS ++A+CGV+ G
Sbjct: 390 FNFYHTEAGRRAPSTLQAFCGVSAG 409

[Close]
BLAST of Cp4.1LG20g02050 vs. TrEMBL
Match: A5AJI0_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_028513 PE=3 SV=1)

HSP 1 Score: 347.8 bits (891), Expect = 1.4e-92
Identity = 167/314 (53.18%), Postives = 223/314 (71.02%), Query Frame = 1 

Query: 2   AGEIPVVRFSSHNLQSGGRKWGSTREKVREALEEYGCFVALYDSVSMEASSLKMMEAMKE 61
           A  +PV+ FS+  L+ G R W    + V  ALEEYGCFVA+YD VS E  +    + ++E
Sbjct: 5   APRLPVLNFSTGTLKPGTRSWLLACKDVLHALEEYGCFVAVYDKVSSELDNTTFCQ-LEE 64

Query: 62  VFEVPLERKVQNVSEKPFHGYFGQNPLMPIHESMGIEDPLLPSNIQSFSNLMWPSRPNPL 121
           +F++P E KV+N S+KP+ GY GQ+PL+P+HESMGIED      ++SF+N+MWP+  N  
Sbjct: 65  LFDLPTETKVKNTSDKPYFGYIGQHPLIPLHESMGIEDATTLKGVESFTNIMWPAG-NDH 124

Query: 122 FCENLKSYAELVSQLEQTVKKMVFESYGLKSLFLSHTQSTKYLLRMIKYRVPHRNEMNLG 181
           FCE + SY++LVS+LEQ VKKMVFESY  +  + SH QST YLLR+IKYR P +NE  +G
Sbjct: 125 FCETVLSYSKLVSELEQMVKKMVFESYDAEKYYDSHIQSTTYLLRLIKYRGPQKNETCIG 184

Query: 182 AFPHTDKSFLTILHQNSVNGLHIKTRDNRWVEYQPPSAESSAKSFIVMAGDAFFAWSNGR 241
              HTDKSF+T+LHQN V GL I+T+D  W+ ++P     S  S++VMAGDA  AWSNGR
Sbjct: 185 TSCHTDKSFITVLHQNEVCGLEIRTKDGEWISFKP-----SPSSYVVMAGDALLAWSNGR 244

Query: 242 IYSAPHRVTMSGNRERYSVGLFSFNDGIVQIPKELVDDKHPQQFKAFDHQDYLRFYSTEK 301
           I+S  HRV M+GN+ RYS+GLF+++ GI++IP+EL D++HP QFK F+H   L FY T K
Sbjct: 245 IHSPEHRVIMNGNKARYSIGLFAYHRGIIEIPEELADEEHPLQFKPFNHYGLLHFYFTNK 304

Query: 302 GQSSPSAIKAYCGV 316
              + S  KAYCGV
Sbjct: 305 AGQTESTAKAYCGV 311

[Close]
BLAST of Cp4.1LG20g02050 vs. TrEMBL
Match: D7U0Q7_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_09s0002g05290 PE=3 SV=1)

HSP 1 Score: 347.8 bits (891), Expect = 1.4e-92
Identity = 167/314 (53.18%), Postives = 223/314 (71.02%), Query Frame = 1 

Query: 2   AGEIPVVRFSSHNLQSGGRKWGSTREKVREALEEYGCFVALYDSVSMEASSLKMMEAMKE 61
           A  +PV+ FS+  L+ G R W    + V  ALEEYGCFVA+YD VS E  +    + ++E
Sbjct: 5   APRLPVLNFSTGTLKPGTRSWLLACKDVLHALEEYGCFVAVYDKVSSELDNTTFCQ-LEE 64

Query: 62  VFEVPLERKVQNVSEKPFHGYFGQNPLMPIHESMGIEDPLLPSNIQSFSNLMWPSRPNPL 121
           +F++P E KV+N S+KP+ GY GQ+PL+P+HESMGIED      ++SF+N+MWP+  N  
Sbjct: 65  LFDLPTETKVKNTSDKPYFGYIGQHPLIPLHESMGIEDATTLKGVESFTNIMWPAG-NDH 124

Query: 122 FCENLKSYAELVSQLEQTVKKMVFESYGLKSLFLSHTQSTKYLLRMIKYRVPHRNEMNLG 181
           FCE + SY++LVS+LEQ VKKMVFESY  +  + SH QST YLLR+IKYR P +NE  +G
Sbjct: 125 FCETVLSYSKLVSELEQMVKKMVFESYDAEKYYDSHIQSTTYLLRLIKYRGPQKNETCIG 184

Query: 182 AFPHTDKSFLTILHQNSVNGLHIKTRDNRWVEYQPPSAESSAKSFIVMAGDAFFAWSNGR 241
              HTDKSF+T+LHQN V GL I+T+D  W+ ++P     S  S++VMAGDA  AWSNGR
Sbjct: 185 TSCHTDKSFITVLHQNEVCGLEIRTKDGEWISFKP-----SPSSYVVMAGDALLAWSNGR 244

Query: 242 IYSAPHRVTMSGNRERYSVGLFSFNDGIVQIPKELVDDKHPQQFKAFDHQDYLRFYSTEK 301
           I+S  HRV M+GN+ RYS+GLF+++ GI++IP+EL D++HP QFK F+H   L FY T K
Sbjct: 245 IHSPEHRVIMNGNKARYSIGLFAYHRGIIEIPEELADEEHPLQFKPFNHYGLLHFYFTNK 304

Query: 302 GQSSPSAIKAYCGV 316
              + S  KAYCGV
Sbjct: 305 AGQAESTAKAYCGV 311

[Close]
BLAST of Cp4.1LG20g02050 vs. TrEMBL
Match: W9STP2_9ROSA (Gibberellin 3-beta-dioxygenase 4 OS=Morus notabilis GN=L484_019467 PE=3 SV=1)

HSP 1 Score: 340.5 bits (872), Expect = 2.3e-90
Identity = 163/313 (52.08%), Postives = 220/313 (70.29%), Query Frame = 1 

Query: 4   EIPVVRFSSHNLQSGGRKWGSTREKVREALEEYGCFVALYDSVSMEASSLKMMEAMKEVF 63
           +IP + FS  NL  G   W S    VR ALEEYGCFVALYD +S +  +  M   +KE+F
Sbjct: 8   KIPTIDFSEGNLNPGTSSWFSVCNDVRRALEEYGCFVALYDKISQQLRT-SMFSELKELF 67

Query: 64  EVPLERKVQNVSEKPFHGYFGQNP-LMPIHESMGIEDPLLPSNIQSFSNLMWPSRPNPLF 123
           ++P E K++NVS+KP+ GY G +P + P+HESMGIE+       +SF+N++WP   N  F
Sbjct: 68  DLPTEIKMKNVSDKPYFGYIGNHPSIPPLHESMGIENATTLEGGESFTNIIWPKGGNNHF 127

Query: 124 CENLKSYAELVSQLEQTVKKMVFESYGLKSLFLSHTQSTKYLLRMIKYRVPHRNEMNLGA 183
           CE + SY+ LVS+LE+ VK+MVF+SYG++  + SH  ST +LL++IKYRVP  NE ++G 
Sbjct: 128 CETVVSYSRLVSELEKMVKRMVFQSYGVEKYYDSHLGSTTFLLKLIKYRVPEMNESSIGC 187

Query: 184 FPHTDKSFLTILHQNSVNGLHIKTRDNRWVEYQPPSAESSAKSFIVMAGDAFFAWSNGRI 243
            PHTDKSF+T+LHQN VNGL I+TRD  W++++P     S  SF+V+AGDAF AW+NGRI
Sbjct: 188 APHTDKSFITVLHQNEVNGLEIETRDGDWIQFEP-----SPSSFLVLAGDAFLAWTNGRI 247

Query: 244 YSAPHRVTMSGNRERYSVGLFSFNDGIVQIPKELVDDKHPQQFKAFDHQDYLRFYSTEKG 303
           +SA HRV M+G + RYS+GLFS+++G ++IP ELVD++HPQQFK+FDH   L  Y     
Sbjct: 248 HSAAHRVVMTGTKPRYSIGLFSYHNGTIEIPDELVDEQHPQQFKSFDHYGLLHHY--HAA 307

Query: 304 QSSPSAIKAYCGV 316
               S  K YCGV
Sbjct: 308 PKIESTAKDYCGV 312

[Close]
BLAST of Cp4.1LG20g02050 vs. TrEMBL
Match: V4RZ66_9ROSI (Uncharacterized protein OS=Citrus clementina GN=CICLE_v10027501mg PE=3 SV=1)

HSP 1 Score: 339.7 bits (870), Expect = 3.9e-90
Identity = 168/314 (53.50%), Postives = 224/314 (71.34%), Query Frame = 1 

Query: 4   EIPVVRFSSHNLQSGGRKWGSTREKVREALEEYGCFVALYDSVSMEASSLKMMEAMKEVF 63
           ++PVV  S  N + G   W  T  KVREALEE+GCFVALYD VS+E  +  +  A +E+F
Sbjct: 31  KLPVVNLSKENFKPGASSWLGTSRKVREALEEFGCFVALYDEVSLELHNA-IFSAAEELF 90

Query: 64  EVPLERKVQNVSEKPFHGYFGQNPLMP-IHESMGIEDPLLPSNIQSFSNLMWPSRPNPLF 123
            +P+E K +NVSEKP++GY G NPL+P I+E MG++        Q+F+NL+WP   N  F
Sbjct: 91  NLPIEIKEKNVSEKPYYGYLGNNPLVPAIYEGMGVDYANTIEGTQNFTNLIWPEG-NENF 150

Query: 124 CENLKSYAELVSQLEQTVKKMVFESYGLKSLFLSHTQSTKYLLRMIKYRVPHRNEMNLGA 183
           C+ + SY+ LVS+LEQ VK+MVFESYG+   + S  +S+ YLLR++KYR P  NE NLG 
Sbjct: 151 CKTVVSYSRLVSELEQMVKRMVFESYGVDKYYDSVLESSTYLLRIMKYRCPETNEKNLGC 210

Query: 184 FPHTDKSFLTILHQNSVNGLHIKTRDNRWVEYQPPSAESSAKSFIVMAGDAFFAWSNGRI 243
             HTDKSF+T+LHQN VNGL I+T+D  W+ +  P    +  SFIV+AGDAF AWSN RI
Sbjct: 211 DVHTDKSFITVLHQNEVNGLEIRTKDGCWIGFDDP----TPSSFIVLAGDAFLAWSNSRI 270

Query: 244 YSAPHRVTMSGNRE-RYSVGLFSFNDGIVQIPKELVDDKHPQQFKAFDHQDYLRFYSTEK 303
           +S  HRV ++G ++ RYS+GLF+FNDGI+QIP+ELVDD+HP +FK+F+H   LR+Y TE+
Sbjct: 271 HSPVHRVILNGIKQARYSLGLFTFNDGIIQIPEELVDDEHPLKFKSFEHFGLLRYYLTEE 330

Query: 304 GQSSPSAIKAYCGV 316
           GQ +    KAYC V
Sbjct: 331 GQKAECTAKAYCSV 338

[Close]
BLAST of Cp4.1LG20g02050 vs. TrEMBL
Match: A0A0A0LT96_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_1G044830 PE=4 SV=1)

HSP 1 Score: 338.6 bits (867), Expect = 8.6e-90
Identity = 168/236 (71.19%), Postives = 197/236 (83.47%), Query Frame = 1 

Query: 4   EIPVVRFSSHNLQSGGRKWGSTREKVREALEEYGCFVALYDSVSMEASSLKMMEAMKEVF 63
           ++PVV+F S NL+SG  +W ST +KVREALEEYGCFVALYD+VS E SS  M +++KE+F
Sbjct: 9   QLPVVKFCSQNLKSGTEEWVSTTKKVREALEEYGCFVALYDTVSAEVSS-NMFDSLKELF 68

Query: 64  -EVPLERKVQNVSEKPFHGYFGQNPLMPIHESMGIEDPLLPSNIQSFSNLMWPSRPNPLF 123
            EVPLERK+QNVSEKP+HGYFGQNPLMPIHESMGIE P+LP+NI SF+NLMWPSR N  F
Sbjct: 69  DEVPLERKLQNVSEKPYHGYFGQNPLMPIHESMGIEHPILPTNINSFTNLMWPSRGNHSF 128

Query: 124 CENLKSYAELVSQLEQTVKKMVFESYGLKSLFLSHTQSTKYLLRMIKYRVPHRNEMNLGA 183
            EN+ +YA LVS+L++ VK MVFESYG+ +   SH +STKYL+RMIKYRVP   EMNLGA
Sbjct: 129 RENVTAYANLVSELDKRVKSMVFESYGVGNALRSHMESTKYLMRMIKYRVPKEKEMNLGA 188

Query: 184 FPHTDKSFLTILHQNSVNGLHIKTRDNRWVEYQPPSAESSAKSFIVMAGDAFFAWS 239
           FPHTDKSFLTILHQN VNGL IKTRDN+W++Y P S+ SS  SFIVMAGDAFF  S
Sbjct: 189 FPHTDKSFLTILHQNEVNGLQIKTRDNKWIQYHPFSS-SSTSSFIVMAGDAFFVSS 242

[Close]
BLAST of Cp4.1LG20g02050 vs. TAIR10
Match: AT1G52800.1 (AT1G52800.1 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein)

HSP 1 Score: 287.0 bits (733), Expect = 1.5e-77
Identity = 138/314 (43.95%), Postives = 210/314 (66.88%), Query Frame = 1 

Query: 4   EIPVVRFSSHN-LQSGGRKWGSTREKVREALEEYGCFVALYDSVSMEASSLKMMEAMKEV 63
           ++P++  +S   L+     W S   +  EALEEYGCF+A+YD V+ +     +  A +E+
Sbjct: 9   KVPILDLTSQQELKPNTSTWRSVSREACEALEEYGCFLAVYDGVTQQLDD-SIFAAAEEL 68

Query: 64  FEVPLERKVQNVSEKPFHGYFGQNPLMPIHESMGIEDPLLPSNIQSFSNLMWPSRPNPLF 123
           F++P E K +NV+EKP+HGY GQ P++P+HE +G++        Q F++LMWP + N  F
Sbjct: 69  FDLPTETKKKNVNEKPYHGYVGQMPVIPLHEGLGVDYVTNKEIAQRFTHLMWP-QGNDRF 128

Query: 124 CENLKSYAELVSQLEQTVKKMVFESYGLKSLFLSHTQSTKYLLRMIKYRVPHRNEMNLGA 183
           C  + +++  V++L++ V +M+FE+YG++  + SH  S  YLL+ +KY  P  + +++ A
Sbjct: 129 CNTVHTFSNAVAELDRLVVRMIFENYGVEKHYESHVGSKTYLLKFLKYLAPPES-ISMPA 188

Query: 184 FP-HTDKSFLTILHQNSVNGLHIKTRDNRWVEYQPPSAESSAKSFIVMAGDAFFAWSNGR 243
           FP HTDK+FL+ILHQN VNGL +K++D  W+  Q P      KS++VMAGD    WSN R
Sbjct: 189 FPQHTDKTFLSILHQNDVNGLEVKSKDGEWISLQLPP-----KSYVVMAGDISMGWSNDR 248

Query: 244 IYSAPHRVTMSGNRERYSVGLFSFNDGIVQIPKELVDDKHPQQFKAFDHQDYLRFYSTEK 303
           I S  HRVTM G++ RY++GLFSF   +V IP+ELVDDKHP  +K FD+   + FY+T++
Sbjct: 249 IRSCEHRVTMEGDKTRYTLGLFSFLTDLVSIPEELVDDKHPLMYKPFDNIALINFYTTKE 308

Query: 304 GQSSPSAIKAYCGV 316
           G+ + S +KAYCG+
Sbjct: 309 GREANSTLKAYCGI 314

[Close]
BLAST of Cp4.1LG20g02050 vs. TAIR10
Match: AT1G52820.1 (AT1G52820.1 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein)

HSP 1 Score: 285.4 bits (729), Expect = 4.4e-77
Identity = 137/313 (43.77%), Postives = 210/313 (67.09%), Query Frame = 1 

Query: 5   IPVVRFSSHNLQSGGRKWGSTREKVREALEEYGCFVALYDSVSMEASSLKMMEAMKEVFE 64
           +PV+ FS+ NL+ G  +W  TR  V++AL++YG F A +D +  E     +  A++E+F+
Sbjct: 12  LPVIDFSNKNLKPGEPEWDLTRADVQKALQDYGYFEASFDRIPFELRK-SVFGALEELFD 71

Query: 65  VPLERKVQNVSEKPFHGYFGQNPLMPIHESMGIEDPLLPSNIQSFSNLMWPSRPNPLFCE 124
           +PL+ K++NVS+KPFHGY GQ P++P++ESMGI+D  +   + +F+  +WP + N  F  
Sbjct: 72  LPLQTKLRNVSKKPFHGYVGQYPMVPLYESMGIDDSDIAEKVDAFTEKLWP-QGNISFST 131

Query: 125 NLKSYAELVSQLEQTVKKMVFESYGLKSLFLSHTQSTKYLLRMIKYRVPHRNEMNLGAFP 184
            ++S+++ +S+L+ T+++M+ ES+GL      H  ST YLLR++KY+ P   E  +G   
Sbjct: 132 TIQSFSKKLSELDITIRRMIMESFGLDKYIDEHLHSTNYLLRVMKYKGPDTEETKVGLNA 191

Query: 185 HTDKSFLTILHQNSVNGLHIKTRDNRWVEYQPPSAESSAKSFIVMAGDAFFAWSNGRIYS 244
           HTDK+ +TIL+QN V GL ++T+D  W++ +P     +  SF VM GD+ +A  NGR++S
Sbjct: 192 HTDKNIVTILYQNHVEGLEVQTKDKNWIKVKP-----TQDSFTVMIGDSLYALLNGRLHS 251

Query: 245 APHRVTMSGNRERYSVGLFSFNDG--IVQIPKELVDDKHPQQFKAFDHQDYLRFYSTEKG 304
             HRV M+G   RYS+GLFS      IV  P ELVD++HP+ FK FDH ++L+FY TE G
Sbjct: 252 PYHRVMMTGTETRYSLGLFSIPKAGHIVSSPDELVDEEHPRLFKPFDHVEFLQFYYTEAG 311

Query: 305 QSSPSAIKAYCGV 316
           Q S SA+K YCG+
Sbjct: 312 QRSQSALKTYCGI 317

[Close]
BLAST of Cp4.1LG20g02050 vs. TAIR10
Match: AT1G52790.1 (AT1G52790.1 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein)

HSP 1 Score: 283.1 bits (723), Expect = 2.2e-76
Identity = 139/315 (44.13%), Postives = 207/315 (65.71%), Query Frame = 1 

Query: 1   MAGEIPVVRFSSHNLQSGGRKWGSTREKVREALEEYGCFVA-LYDSVSMEASSLKMMEAM 60
           M+ +IP + FS  +L+ G + W STRE +R+ALEEYGCF+  L D   ++    ++  ++
Sbjct: 1   MSPKIPTLDFSREDLKPGTKYWESTRENIRQALEEYGCFIIDLKDKTPLDLLD-RVFGSL 60

Query: 61  KEVFEVPLERKVQNVSEKPFHGYFGQNPLMPIHESMGIEDPLLPSNIQSFSNLMWPSRPN 120
            ++F++P + K++N  +KP +GY GQ P +P+HES+GI++       +SF+ LMWP + N
Sbjct: 61  VDLFDLPTQTKMKNKYDKPLNGYVGQIPALPLHESLGIDNATSLEATRSFTGLMWP-QGN 120

Query: 121 PLFCENLKSYAELVSQLEQTVKKMVFESYGLKSLFLSHTQSTKYLLRMIKYRVPHRNEMN 180
             F E L  YAE  ++L+Q V +MVF+SY ++  +  + +ST YLLR++K R P+     
Sbjct: 121 EHFSECLYKYAEFAAELDQMVTRMVFQSYNVEKYYDPYIESTTYLLRVLKNRAPNNENPT 180

Query: 181 LGAFPHTDKSFLTILHQNSVNGLHIKTRDNRWVEYQPPSAESSAKSFIVMAGDAFFAWSN 240
           LG   HTDKSF TILHQ+ VNGL ++TR+   +        SS   F+V+AGDA  AWSN
Sbjct: 181 LGFVTHTDKSFTTILHQDQVNGLEMETREGERININL----SSPSLFMVVAGDALMAWSN 240

Query: 241 GRIYSAPHRVTMSGNRERYSVGLFSFNDGIVQIPKELVDDKHPQQFKAFDHQDYLRFYST 300
            R++S  H+V +SG  +RYS+G+F+FN+G +Q+P+EL+D +HP  +K FDH   LRFY T
Sbjct: 241 DRVWSPRHQVLVSGETDRYSLGMFAFNNGTLQVPEELIDHQHPLMYKPFDHLGLLRFYRT 300

Query: 301 EKGQSSPSAIKAYCG 315
           + G  S   IKAYCG
Sbjct: 301 DTGYKSECPIKAYCG 309

[Close]
BLAST of Cp4.1LG20g02050 vs. TAIR10
Match: AT4G03070.1 (AT4G03070.1 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein)

HSP 1 Score: 251.1 bits (640), Expect = 9.2e-67
Identity = 127/317 (40.06%), Postives = 195/317 (61.51%), Query Frame = 1 

Query: 4   EIPVVRFSSHNLQSGGRKWGSTREKVREALEEYGCFVALYDSVSMEASSLKMMEAMKEVF 63
           ++PV+ FS  NL+ G  KW      V +ALE+YGCF A +D +S+E +   + EAM+++F
Sbjct: 13  QLPVIDFSDQNLKPGSSKWDEVTADVLKALEDYGCFEASFDKLSVELNR-SVFEAMEDLF 72

Query: 64  EVPLERKVQNVSEKPFHGYFGQNPLMPIHESMGIEDPLLPSNIQSFSNLMWPSRPNPLFC 123
           E+P+  K +NVS KPFHGY   N    ++ES+GI D  +   +  F+  +WP   N    
Sbjct: 73  ELPIPTKQRNVSSKPFHGYLCHN----LYESLGINDANVLEKVNDFTQQLWPDHGNKSIS 132

Query: 124 ENLKSYAELVSQLEQTVKKMVFESYGLKSLFLSHTQSTKYLLRMIKYRVPHRN------E 183
           E +  ++E + +L+  V++M+ ES+G+++    H  ST YL R++KY  P  +      E
Sbjct: 133 ETIHLFSEQLVELDLMVRRMIMESFGIENYIDEHLNSTYYLTRLMKYTSPPDDDDDDDEE 192

Query: 184 MNLGAFPHTDKSFLTILHQNSVNGLHIKTRDNRWVEYQPPSAESSAKSFIVMAGDAFFAW 243
             LG   HTDK+ +TILHQ  V+GL +KT+D++W++ +P     S  S +VM GD+  A 
Sbjct: 193 TKLGLRSHTDKNIITILHQYQVDGLEVKTKDDKWIKVKP-----SQDSVLVMVGDSLCAL 252

Query: 244 SNGRIYSAPHRVTMSGNRERYSVGLFSF--NDGIVQIPKELVDDKHPQQFKAFDHQDYLR 303
            NGR++S  HRV M+G + RYS GLFS      I+  P+ELVD +HP+ FK F++ D+L 
Sbjct: 253 LNGRLHSPYHRVIMTGKKTRYSTGLFSIPKTGVIIDSPEELVDKEHPRIFKPFEYTDFLH 312

Query: 304 FYSTEKGQSSPSAIKAY 313
           F+ TE G+ + SA+ A+
Sbjct: 313 FFQTEAGRIAQSALHAF 319

[Close]
BLAST of Cp4.1LG20g02050 vs. TAIR10
Match: AT1G80320.1 (AT1G80320.1 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein)

HSP 1 Score: 243.4 bits (620), Expect = 1.9e-64
Identity = 130/317 (41.01%), Postives = 190/317 (59.94%), Query Frame = 1 

Query: 5   IPVVRFSSHNLQSGGRKWGSTREKVREALEEYGCFVALYDSVSMEASSLKMMEAMKEVFE 64
           +P++ FSS  L  G   W +TR+ VR A+E  G FVA +  VS +     ++  MKE++ 
Sbjct: 9   LPILDFSSDKLVRGTSHWITTRDDVRRAMEGQGWFVAEFSGVSSDLRD-NLLAGMKEMYY 68

Query: 65  VPLERKVQNVSEKPFHGYFGQN-PLMPIHESMGIEDPLLPSNIQSFSNLMWPSRPNPLFC 124
           +P + K++N + K  HGY         IHES+GI+        + FS L+WP   +P FC
Sbjct: 69  LPDQIKIKNENHKASHGYMSMVVDDYRIHESLGIDYATELQACKDFSKLLWPQGNDP-FC 128

Query: 125 ENLKSYAELVSQLEQTVKKMVFESYGL--KSLFLSHTQSTKYLLRMIKYRVPHRNEMNLG 184
           +    YA  +++L+QTV +M++ESYG+  K   +SH++ST+YLLRM+ YR     E N G
Sbjct: 129 QTTHMYAMTMAELDQTVMRMLYESYGMDEKKHSVSHSESTRYLLRMLSYRRQQNGEANTG 188

Query: 185 AFPHTDKSFLTILHQNSVNGLHIKTRDNRWVEYQPPSAESSAKSFIVMAGDAFFAWSNGR 244
              HTDKSF++ILHQN V GL +KT   +WV + P     S   F+V++G    AWSN R
Sbjct: 189 FVSHTDKSFMSILHQNHVGGLQLKTMTGQWVGFNP-----SPTRFVVLSGMGLTAWSNDR 248

Query: 245 IYSAPHRVTMSGNRERYSVGLFSFNDGIVQIPKELVDDKHPQQFKAFDHQDYLRFYST-- 304
           I +  H+V MS +  RYS+G FSF+ G ++ P+ELVDD+HP ++  F+H   LRFY +  
Sbjct: 249 IKACYHKVVMSADEIRYSLGFFSFHKGTIRTPEELVDDQHPLRYNPFEHDGLLRFYESYI 308

Query: 305 -EKGQSSPSAIKAYCGV 316
               +SS   ++ YCGV
Sbjct: 309 NSLKKSSEDLLQIYCGV 318

[Close]
BLAST of Cp4.1LG20g02050 vs. NCBI nr
Match: gi|778664781|ref|XP_011660352.1| (PREDICTED: probable 2-oxoglutarate-dependent dioxygenase AOP1 [Cucumis sativus])

HSP 1 Score: 499.6 bits (1285), Expect = 4.2e-138
Identity = 243/326 (74.54%), Postives = 279/326 (85.58%), Query Frame = 1 

Query: 4   EIPVVRFSSHNLQSGGRKWGSTREKVREALEEYGCFVALYDSVSMEASSLKMMEAMKEVF 63
           ++PVV+F S NL+SG  +W ST +KVREALEEYGCFVALYD+VS E SS  M +++KE+F
Sbjct: 9   QLPVVKFCSQNLKSGTEEWVSTTKKVREALEEYGCFVALYDTVSAEVSS-NMFDSLKELF 68

Query: 64  -EVPLERKVQNVSEKPFHGYFGQNPLMPIHESMGIEDPLLPSNIQSFSNLMWPSRPNPLF 123
            EVPLERK+QNVSEKP+HGYFGQNPLMPIHESMGIE P+LP+NI SF+NLMWPSR N  F
Sbjct: 69  DEVPLERKLQNVSEKPYHGYFGQNPLMPIHESMGIEHPILPTNINSFTNLMWPSRGNHSF 128

Query: 124 CENLKSYAELVSQLEQTVKKMVFESYGLKSLFLSHTQSTKYLLRMIKYRVPHRNEMNLGA 183
            EN+ +YA LVS+L++ VK MVFESYG+ +   SH +STKYL+RMIKYRVP   EMNLGA
Sbjct: 129 RENVTAYANLVSELDKRVKSMVFESYGVGNALRSHMESTKYLMRMIKYRVPKEKEMNLGA 188

Query: 184 FPHTDKSFLTILHQNSVNGLHIKTRDNRWVEYQPPSAESSAKSFIVMAGDAFFAWSNGRI 243
           FPHTDKSFLTILHQN VNGL IKTRDN+W++Y P S+ SS  SFIVMAGDAFFAWSNGRI
Sbjct: 189 FPHTDKSFLTILHQNEVNGLQIKTRDNKWIQYHPFSS-SSTSSFIVMAGDAFFAWSNGRI 248

Query: 244 YSAPHRVTMSGNRERYSVGLFSFNDGIVQIPKELVDDKHPQQFKAFDHQDYLRFYSTEKG 303
           YS PHRV MSGN+ERYSVGLFSFN+GIVQIPKELVDDKHPQ FK FDHQDYLRFYSTE+G
Sbjct: 249 YSPPHRVIMSGNKERYSVGLFSFNNGIVQIPKELVDDKHPQLFKPFDHQDYLRFYSTERG 308

Query: 304 QSSPSAIKAYCGVANGLLDSRTCIPM 329
           Q+SP AIKAYCGV N L+D+ T  P+
Sbjct: 309 QNSPCAIKAYCGVGNSLIDTDTRSPL 332

[Close]
BLAST of Cp4.1LG20g02050 vs. NCBI nr
Match: gi|778657573|ref|XP_004151475.2| (PREDICTED: probable 2-oxoglutarate-dependent dioxygenase AOP1.2 [Cucumis sativus])

HSP 1 Score: 434.5 bits (1116), Expect = 1.7e-118
Identity = 209/275 (76.00%), Postives = 238/275 (86.55%), Query Frame = 1 

Query: 55  MMEAMKEVF-EVPLERKVQNVSEKPFHGYFGQNPLMPIHESMGIEDPLLPSNIQSFSNLM 114
           M +++KE+F EVPLERK+QNVSEKP+HGYFGQNPLMPIHESMGIE P+LP+NI SF+NLM
Sbjct: 1   MFDSLKELFDEVPLERKLQNVSEKPYHGYFGQNPLMPIHESMGIEHPILPTNINSFTNLM 60

Query: 115 WPSRPNPLFCENLKSYAELVSQLEQTVKKMVFESYGLKSLFLSHTQSTKYLLRMIKYRVP 174
           WPSR N  F EN+ +YA LVS+L++ VK MVFESYG+ +   SH +STKYL+RMIKYRVP
Sbjct: 61  WPSRGNHSFRENVTAYANLVSELDKRVKSMVFESYGVGNALRSHMESTKYLMRMIKYRVP 120

Query: 175 HRNEMNLGAFPHTDKSFLTILHQNSVNGLHIKTRDNRWVEYQPPSAESSAKSFIVMAGDA 234
              EMNLGAFPHTDKSFLTILHQN VNGL IKTRDN+W++Y P S+ SS  SFIVMAGDA
Sbjct: 121 KEKEMNLGAFPHTDKSFLTILHQNEVNGLQIKTRDNKWIQYHPFSS-SSTSSFIVMAGDA 180

Query: 235 FFAWSNGRIYSAPHRVTMSGNRERYSVGLFSFNDGIVQIPKELVDDKHPQQFKAFDHQDY 294
           FFAWSNGRIYS PHRV MSGN+ERYSVGLFSFN+GIVQIPKELVDDKHPQ FK FDHQDY
Sbjct: 181 FFAWSNGRIYSPPHRVIMSGNKERYSVGLFSFNNGIVQIPKELVDDKHPQLFKPFDHQDY 240

Query: 295 LRFYSTEKGQSSPSAIKAYCGVANGLLDSRTCIPM 329
           LRFYSTE+GQ+SP AIKAYCGV N L+D+ T  P+
Sbjct: 241 LRFYSTERGQNSPCAIKAYCGVGNSLIDTDTRSPL 274

[Close]
BLAST of Cp4.1LG20g02050 vs. NCBI nr
Match: gi|147852210|emb|CAN80139.1| (hypothetical protein VITISV_028513 [Vitis vinifera])

HSP 1 Score: 347.8 bits (891), Expect = 2.0e-92
Identity = 167/314 (53.18%), Postives = 223/314 (71.02%), Query Frame = 1 

Query: 2   AGEIPVVRFSSHNLQSGGRKWGSTREKVREALEEYGCFVALYDSVSMEASSLKMMEAMKE 61
           A  +PV+ FS+  L+ G R W    + V  ALEEYGCFVA+YD VS E  +    + ++E
Sbjct: 5   APRLPVLNFSTGTLKPGTRSWLLACKDVLHALEEYGCFVAVYDKVSSELDNTTFCQ-LEE 64

Query: 62  VFEVPLERKVQNVSEKPFHGYFGQNPLMPIHESMGIEDPLLPSNIQSFSNLMWPSRPNPL 121
           +F++P E KV+N S+KP+ GY GQ+PL+P+HESMGIED      ++SF+N+MWP+  N  
Sbjct: 65  LFDLPTETKVKNTSDKPYFGYIGQHPLIPLHESMGIEDATTLKGVESFTNIMWPAG-NDH 124

Query: 122 FCENLKSYAELVSQLEQTVKKMVFESYGLKSLFLSHTQSTKYLLRMIKYRVPHRNEMNLG 181
           FCE + SY++LVS+LEQ VKKMVFESY  +  + SH QST YLLR+IKYR P +NE  +G
Sbjct: 125 FCETVLSYSKLVSELEQMVKKMVFESYDAEKYYDSHIQSTTYLLRLIKYRGPQKNETCIG 184

Query: 182 AFPHTDKSFLTILHQNSVNGLHIKTRDNRWVEYQPPSAESSAKSFIVMAGDAFFAWSNGR 241
              HTDKSF+T+LHQN V GL I+T+D  W+ ++P     S  S++VMAGDA  AWSNGR
Sbjct: 185 TSCHTDKSFITVLHQNEVCGLEIRTKDGEWISFKP-----SPSSYVVMAGDALLAWSNGR 244

Query: 242 IYSAPHRVTMSGNRERYSVGLFSFNDGIVQIPKELVDDKHPQQFKAFDHQDYLRFYSTEK 301
           I+S  HRV M+GN+ RYS+GLF+++ GI++IP+EL D++HP QFK F+H   L FY T K
Sbjct: 245 IHSPEHRVIMNGNKARYSIGLFAYHRGIIEIPEELADEEHPLQFKPFNHYGLLHFYFTNK 304

Query: 302 GQSSPSAIKAYCGV 316
              + S  KAYCGV
Sbjct: 305 AGQTESTAKAYCGV 311

[Close]
BLAST of Cp4.1LG20g02050 vs. NCBI nr
Match: gi|225442749|ref|XP_002284974.1| (PREDICTED: probable 2-oxoglutarate-dependent dioxygenase AOP1 [Vitis vinifera])

HSP 1 Score: 347.8 bits (891), Expect = 2.0e-92
Identity = 167/314 (53.18%), Postives = 223/314 (71.02%), Query Frame = 1 

Query: 2   AGEIPVVRFSSHNLQSGGRKWGSTREKVREALEEYGCFVALYDSVSMEASSLKMMEAMKE 61
           A  +PV+ FS+  L+ G R W    + V  ALEEYGCFVA+YD VS E  +    + ++E
Sbjct: 5   APRLPVLNFSTGTLKPGTRSWLLACKDVLHALEEYGCFVAVYDKVSSELDNTTFCQ-LEE 64

Query: 62  VFEVPLERKVQNVSEKPFHGYFGQNPLMPIHESMGIEDPLLPSNIQSFSNLMWPSRPNPL 121
           +F++P E KV+N S+KP+ GY GQ+PL+P+HESMGIED      ++SF+N+MWP+  N  
Sbjct: 65  LFDLPTETKVKNTSDKPYFGYIGQHPLIPLHESMGIEDATTLKGVESFTNIMWPAG-NDH 124

Query: 122 FCENLKSYAELVSQLEQTVKKMVFESYGLKSLFLSHTQSTKYLLRMIKYRVPHRNEMNLG 181
           FCE + SY++LVS+LEQ VKKMVFESY  +  + SH QST YLLR+IKYR P +NE  +G
Sbjct: 125 FCETVLSYSKLVSELEQMVKKMVFESYDAEKYYDSHIQSTTYLLRLIKYRGPQKNETCIG 184

Query: 182 AFPHTDKSFLTILHQNSVNGLHIKTRDNRWVEYQPPSAESSAKSFIVMAGDAFFAWSNGR 241
              HTDKSF+T+LHQN V GL I+T+D  W+ ++P     S  S++VMAGDA  AWSNGR
Sbjct: 185 TSCHTDKSFITVLHQNEVCGLEIRTKDGEWISFKP-----SPSSYVVMAGDALLAWSNGR 244

Query: 242 IYSAPHRVTMSGNRERYSVGLFSFNDGIVQIPKELVDDKHPQQFKAFDHQDYLRFYSTEK 301
           I+S  HRV M+GN+ RYS+GLF+++ GI++IP+EL D++HP QFK F+H   L FY T K
Sbjct: 245 IHSPEHRVIMNGNKARYSIGLFAYHRGIIEIPEELADEEHPLQFKPFNHYGLLHFYFTNK 304

Query: 302 GQSSPSAIKAYCGV 316
              + S  KAYCGV
Sbjct: 305 AGQAESTAKAYCGV 311

[Close]
BLAST of Cp4.1LG20g02050 vs. NCBI nr
Match: gi|703152173|ref|XP_010110313.1| (Gibberellin 3-beta-dioxygenase 4 [Morus notabilis])

HSP 1 Score: 340.5 bits (872), Expect = 3.3e-90
Identity = 163/313 (52.08%), Postives = 220/313 (70.29%), Query Frame = 1 

Query: 4   EIPVVRFSSHNLQSGGRKWGSTREKVREALEEYGCFVALYDSVSMEASSLKMMEAMKEVF 63
           +IP + FS  NL  G   W S    VR ALEEYGCFVALYD +S +  +  M   +KE+F
Sbjct: 8   KIPTIDFSEGNLNPGTSSWFSVCNDVRRALEEYGCFVALYDKISQQLRT-SMFSELKELF 67

Query: 64  EVPLERKVQNVSEKPFHGYFGQNP-LMPIHESMGIEDPLLPSNIQSFSNLMWPSRPNPLF 123
           ++P E K++NVS+KP+ GY G +P + P+HESMGIE+       +SF+N++WP   N  F
Sbjct: 68  DLPTEIKMKNVSDKPYFGYIGNHPSIPPLHESMGIENATTLEGGESFTNIIWPKGGNNHF 127

Query: 124 CENLKSYAELVSQLEQTVKKMVFESYGLKSLFLSHTQSTKYLLRMIKYRVPHRNEMNLGA 183
           CE + SY+ LVS+LE+ VK+MVF+SYG++  + SH  ST +LL++IKYRVP  NE ++G 
Sbjct: 128 CETVVSYSRLVSELEKMVKRMVFQSYGVEKYYDSHLGSTTFLLKLIKYRVPEMNESSIGC 187

Query: 184 FPHTDKSFLTILHQNSVNGLHIKTRDNRWVEYQPPSAESSAKSFIVMAGDAFFAWSNGRI 243
            PHTDKSF+T+LHQN VNGL I+TRD  W++++P     S  SF+V+AGDAF AW+NGRI
Sbjct: 188 APHTDKSFITVLHQNEVNGLEIETRDGDWIQFEP-----SPSSFLVLAGDAFLAWTNGRI 247

Query: 244 YSAPHRVTMSGNRERYSVGLFSFNDGIVQIPKELVDDKHPQQFKAFDHQDYLRFYSTEKG 303
           +SA HRV M+G + RYS+GLFS+++G ++IP ELVD++HPQQFK+FDH   L  Y     
Sbjct: 248 HSAAHRVVMTGTKPRYSIGLFSYHNGTIEIPDELVDEQHPQQFKSFDHYGLLHHY--HAA 307

Query: 304 QSSPSAIKAYCGV 316
               S  K YCGV
Sbjct: 308 PKIESTAKDYCGV 312

The following BLAST results are available for this feature:
BLAST of Cp4.1LG20g02050 vs. Swiss-Prot
Match NameE-valueIdentityDescription
AOP1L_ARATH1.9e-6640.19Probable 2-oxoglutarate-dependent dioxygenase AOP1.2 OS=Arabidopsis thaliana GN=... [more]
AOP1C_ARATH1.6e-6540.06Probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Arabidopsis thaliana GN=AO... [more]
AOP1V_ARATH9.9e-6340.20Probable 2-oxoglutarate-dependent dioxygenase AOP1 (Fragment) OS=Arabidopsis tha... [more]
AOP2V_ARATH1.0e-2733.512-oxoglutarate-dependent dioxygenase AOP2 OS=Arabidopsis thaliana GN=AOP2 PE=2 S... [more]
AOP3L_ARATH2.3e-2743.452-oxoglutarate-dependent dioxygenase AOP3 OS=Arabidopsis thaliana GN=AOP3 PE=2 S... [more]
BLAST of Cp4.1LG20g02050 vs. TrEMBL
Match NameE-valueIdentityDescription
A5AJI0_VITVI1.4e-9253.18Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_028513 PE=3 SV=1[more]
D7U0Q7_VITVI1.4e-9253.18Putative uncharacterized protein OS=Vitis vinifera GN=VIT_09s0002g05290 PE=3 SV=... [more]
W9STP2_9ROSA2.3e-9052.08Gibberellin 3-beta-dioxygenase 4 OS=Morus notabilis GN=L484_019467 PE=3 SV=1[more]
V4RZ66_9ROSI3.9e-9053.50Uncharacterized protein OS=Citrus clementina GN=CICLE_v10027501mg PE=3 SV=1[more]
A0A0A0LT96_CUCSA8.6e-9071.19Uncharacterized protein OS=Cucumis sativus GN=Csa_1G044830 PE=4 SV=1[more]
BLAST of Cp4.1LG20g02050 vs. TAIR10
Match NameE-valueIdentityDescription
AT1G52800.11.5e-7743.95 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily prot... [more]
AT1G52820.14.4e-7743.77 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily prot... [more]
AT1G52790.12.2e-7644.13 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily prot... [more]
AT4G03070.19.2e-6740.06 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily prot... [more]
AT1G80320.11.9e-6441.01 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily prot... [more]
BLAST of Cp4.1LG20g02050 vs. NCBI nr
Match NameE-valueIdentityDescription
gi|778664781|ref|XP_011660352.1|4.2e-13874.54PREDICTED: probable 2-oxoglutarate-dependent dioxygenase AOP1 [Cucumis sativus][more]
gi|778657573|ref|XP_004151475.2|1.7e-11876.00PREDICTED: probable 2-oxoglutarate-dependent dioxygenase AOP1.2 [Cucumis sativus... [more]
gi|147852210|emb|CAN80139.1|2.0e-9253.18hypothetical protein VITISV_028513 [Vitis vinifera][more]
gi|225442749|ref|XP_002284974.1|2.0e-9253.18PREDICTED: probable 2-oxoglutarate-dependent dioxygenase AOP1 [Vitis vinifera][more]
gi|703152173|ref|XP_010110313.1|3.3e-9052.08Gibberellin 3-beta-dioxygenase 4 [Morus notabilis][more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: Biological Process
TermDefinition
GO:0055114oxidation-reduction process
Vocabulary: Molecular Function
TermDefinition
GO:0016491oxidoreductase activity
Vocabulary: INTERPRO
TermDefinition
IPR027443IPNS-like
IPR026992DIOX_N
IPR005123Oxoglu/Fe-dep_dioxygenase
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0055114 oxidation-reduction process
cellular_component GO:0005575 cellular_component
molecular_function GO:0016706 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, 2-oxoglutarate as one donor, and incorporation of one atom each of oxygen into both donors
molecular_function GO:0046872 metal ion binding
molecular_function GO:0016491 oxidoreductase activity
molecular_function GO:0051213 dioxygenase activity
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG20g02050.1Cp4.1LG20g02050.1mRNA


InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo
Date Performed: 2017-12-02
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR005123Oxoglutarate/iron-dependent dioxygenasePFAMPF031712OG-FeII_Oxycoord: 169..265
score: 4.3
IPR005123Oxoglutarate/iron-dependent dioxygenasePROFILEPS51471FE2OG_OXYcoord: 161..268
score: 10
IPR026992Non-haem dioxygenase N-terminal domainPFAMPF14226DIOX_Ncoord: 5..84
score: 3.
IPR027443Isopenicillin N synthase-likeGENE3DG3DSA:2.60.120.330coord: 4..303
score: 6.6
NoneNo IPR availablePANTHERPTHR10209OXIDOREDUCTASE, 2OG-FE II OXYGENASE FAMILY PROTEINcoord: 4..307
score: 1.0E
NoneNo IPR availablePANTHERPTHR10209:SF1742-OXOGLUTARATE (2OG) AND FE(II)-DEPENDENT OXYGENASE SUPERFAMILY PROTEIN-RELATEDcoord: 4..307
score: 1.0E
NoneNo IPR availableunknownSSF51197Clavaminate synthase-likecoord: 4..302
score: 8.24