CmoCh13G009720 (gene) Cucurbita moschata (Rifu)

NameCmoCh13G009720
Typegene
OrganismCucurbita moschata (Cucurbita moschata (Rifu))
Description(2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein)
LocationCmo_Chr13 : 8374833 .. 8376008 (-)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSexonthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCGGGTGAGCTTCCTGTTGTGAGATTTAGTTCACACAATTTGCAGAGCGGAGGGAGGAAGTGGGGTTCGACGAGGGAGAAGGTCCGGGAAGCACTTGAAGAATATGGGTGCTTTGTAGCGTTGTGCGACAGCGTTTCAATGGAGGCGAGTTCTTTGAAGATGATGGAAGCCATGGAGGAGGTGTTCGAAGTTCCATTGGAGAGGAAGGTTCAGAATGTGTCTGAGAAGCCTTTCCATGGCTACTTTGGGCAGAACCCATTGATGCCTATCCATGAAAGCATGGGCATTGAAGACCCTCTTCTTCCCTCCAACATCCAATCTTTCTCCAACCTCATGTGGCCCTCTCGCCCAAACCCTCTGTTCTGGTAAATTTCTTCCCTTCCTTCATTCTGCCTTTATTTCTCACTTTCAGAGCTAACCCACATACTATTTTGCAGTGAAAACCTCAAATCCTACGCAGAATTGGTTTCCCAATTGGAGCAGACGGTGAAGAAGATGGTGTTCGAGAGCTACGGCCTCAAAAGCCTCTTCCTTTCTCACACCCAATCCACCAAATATCTTCTCCGGATGATCAAATACAGAGTCCCACATCGGAACGAGATGAATTTGGGGGCTTTCCCTCACACCGACAAGAGCTTCCTCACCATTCTTCATCAGAATTCAGTAAATGGGTTACACATCAAGACCAGAGACAACAGATGGGTTGAATATCAGCCTCCTTCTGCTGAATCTTCTGCCAAATCATTCATCATCATGGCTGGAGATGCCTTCTTTGTAAGTTTCCATTAACTTCAAATTCCATTACAACTAGCAATAACCCATCAAAAACTTACAAACAATTGTGGGGTTGTTGGGACAGGCGTGGAGCAATGGGAGGATATACTCGGCGCCGCACAGAGTGACGATGAGTGGAAACAGAGAGAGATACTCAGTGGGGCTGTTTTCGTTCAACGATGGGATTGTTCAGATTCCAAAAGAATTGGTGGACGACAAACATCCACAGCAGTTTAAGGCGTTTGATCATCAAGATTACCTTCGTTTCTACAGCACTGAGAAAGGCCAAAGCTCCCCTTCTGCTATTAAAGCCTATTGCGGCGTTGCAAATGGCCTCCTTGATTCACGAACTTGCATTCCCATGCATTAACTATTAACCCATCAAATTCCCATTTTCAG

mRNA sequence

ATGGCGGGTGAGCTTCCTGTTGTGAGATTTAGTTCACACAATTTGCAGAGCGGAGGGAGGAAGTGGGGTTCGACGAGGGAGAAGGTCCGGGAAGCACTTGAAGAATATGGGTGCTTTGTAGCGTTGTGCGACAGCGTTTCAATGGAGGCGAGTTCTTTGAAGATGATGGAAGCCATGGAGGAGGTGTTCGAAGTTCCATTGGAGAGGAAGGTTCAGAATGTGTCTGAGAAGCCTTTCCATGGCTACTTTGGGCAGAACCCATTGATGCCTATCCATGAAAGCATGGGCATTGAAGACCCTCTTCTTCCCTCCAACATCCAATCTTTCTCCAACCTCATGTGGCCCTCTCGCCCAAACCCTCTTGAAAACCTCAAATCCTACGCAGAATTGGTTTCCCAATTGGAGCAGACGGTGAAGAAGATGGTGTTCGAGAGCTACGGCCTCAAAAGCCTCTTCCTTTCTCACACCCAATCCACCAAATATCTTCTCCGGATGATCAAATACAGAGTCCCACATCGGAACGAGATGAATTTGGGGGCTTTCCCTCACACCGACAAGAGCTTCCTCACCATTCTTCATCAGAATTCAGTAAATGGGTTACACATCAAGACCAGAGACAACAGATGGGTTGAATATCAGCCTCCTTCTGCTGAATCTTCTGCCAAATCATTCATCATCATGGCTGGAGATGCCTTCTTTGCGTGGAGCAATGGGAGGATATACTCGGCGCCGCACAGAGTGACGATGAGTGGAAACAGAGAGAGATACTCAGTGGGGCTGTTTTCGTTCAACGATGGGATTGTTCAGATTCCAAAAGAATTGGTGGACGACAAACATCCACAGCAGTTTAAGGCGTTTGATCATCAAGATTACCTTCGTTTCTACAGCACTGAGAAAGGCCAAAGCTCCCCTTCTGCTATTAAAGCCTATTGCGGCGTTGCAAATGGCCTCCTTGATTCACGAACTTGCATTCCCATGCATTAACTATTAACCCATCAAATTCCCATTTTCAG

Coding sequence (CDS)

ATGGCGGGTGAGCTTCCTGTTGTGAGATTTAGTTCACACAATTTGCAGAGCGGAGGGAGGAAGTGGGGTTCGACGAGGGAGAAGGTCCGGGAAGCACTTGAAGAATATGGGTGCTTTGTAGCGTTGTGCGACAGCGTTTCAATGGAGGCGAGTTCTTTGAAGATGATGGAAGCCATGGAGGAGGTGTTCGAAGTTCCATTGGAGAGGAAGGTTCAGAATGTGTCTGAGAAGCCTTTCCATGGCTACTTTGGGCAGAACCCATTGATGCCTATCCATGAAAGCATGGGCATTGAAGACCCTCTTCTTCCCTCCAACATCCAATCTTTCTCCAACCTCATGTGGCCCTCTCGCCCAAACCCTCTTGAAAACCTCAAATCCTACGCAGAATTGGTTTCCCAATTGGAGCAGACGGTGAAGAAGATGGTGTTCGAGAGCTACGGCCTCAAAAGCCTCTTCCTTTCTCACACCCAATCCACCAAATATCTTCTCCGGATGATCAAATACAGAGTCCCACATCGGAACGAGATGAATTTGGGGGCTTTCCCTCACACCGACAAGAGCTTCCTCACCATTCTTCATCAGAATTCAGTAAATGGGTTACACATCAAGACCAGAGACAACAGATGGGTTGAATATCAGCCTCCTTCTGCTGAATCTTCTGCCAAATCATTCATCATCATGGCTGGAGATGCCTTCTTTGCGTGGAGCAATGGGAGGATATACTCGGCGCCGCACAGAGTGACGATGAGTGGAAACAGAGAGAGATACTCAGTGGGGCTGTTTTCGTTCAACGATGGGATTGTTCAGATTCCAAAAGAATTGGTGGACGACAAACATCCACAGCAGTTTAAGGCGTTTGATCATCAAGATTACCTTCGTTTCTACAGCACTGAGAAAGGCCAAAGCTCCCCTTCTGCTATTAAAGCCTATTGCGGCGTTGCAAATGGCCTCCTTGATTCACGAACTTGCATTCCCATGCATTAA
BLAST of CmoCh13G009720 vs. Swiss-Prot
Match: AOP1L_ARATH (Probable 2-oxoglutarate-dependent dioxygenase AOP1.2 OS=Arabidopsis thaliana GN=AOP1.2 PE=2 SV=1)

HSP 1 Score: 248.8 bits (634), Expect = 8.0e-65
Identity = 128/316 (40.51%), Postives = 195/316 (61.71%), Query Frame = 1

Query: 4   ELPVVRFSSHNLQSGGRKWGSTREKVREALEEYGCFVALCDSVSMEASSLKMMEAMEEVF 63
           +LPV+ FS  NL+ G  KW   +  V +ALE+YGCF A  D +S+E +   + EAME++F
Sbjct: 13  QLPVIDFSDQNLKPGSSKWDEVKADVLKALEDYGCFEAFFDKLSVELNR-SVFEAMEDLF 72

Query: 64  EVPLERKVQNVSEKPFHGYFGQNPLMPIHESMGIEDPLLPSNIQSFSNLMWPSRPNP--L 123
           E+P+  K +NVS KPFHGY   N    ++ES+GI+D  +   +  F+  +WP   N    
Sbjct: 73  ELPIPTKQRNVSSKPFHGYLCHN----LYESLGIDDANVLEKVNDFTQQLWPDHGNKSIS 132

Query: 124 ENLKSYAELVSQLEQTVKKMVFESYGLKSLFLSHTQSTKYLLRMIKYRVP-----HRNEM 183
           E +  ++E + +L+  V++M+ ES+G+++    H  ST YL R++KY  P        E 
Sbjct: 133 ETIHLFSEQLVELDLMVRRMIMESFGIENYIDEHLNSTYYLTRLMKYTSPPDDDDDDEET 192

Query: 184 NLGAFPHTDKSFLTILHQNSVNGLHIKTRDNRWVEYQPPSAESSAKSFIIMAGDAFFAWS 243
            LG   HTDK+ +TILHQ  V+GL +KT+D++W++ +P     S  S ++M GD+  A  
Sbjct: 193 KLGLRSHTDKNIITILHQYQVDGLEVKTKDDKWIKVKP-----SQDSVLVMVGDSLCALL 252

Query: 244 NGRIYSAPHRVTMSGNRERYSVGLFSF--NDGIVQIPKELVDDKHPQQFKAFDHQDYLRF 303
           NGR++S  HRV M+G + RYS GLFS      I+  P+ELVD +HP+ FK F++ D+L F
Sbjct: 253 NGRLHSPYHRVIMTGKKTRYSTGLFSIPKTGVIIDSPEELVDKEHPRIFKPFEYTDFLHF 312

Query: 304 YSTEKGQSSPSAIKAY 311
           + TE G+ + SA+ A+
Sbjct: 313 FQTEAGRIAQSALHAF 318

BLAST of CmoCh13G009720 vs. Swiss-Prot
Match: AOP1C_ARATH (Probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Arabidopsis thaliana GN=AOP1 PE=2 SV=1)

HSP 1 Score: 245.7 bits (626), Expect = 6.8e-64
Identity = 128/317 (40.38%), Postives = 194/317 (61.20%), Query Frame = 1

Query: 4   ELPVVRFSSHNLQSGGRKWGSTREKVREALEEYGCFVALCDSVSMEASSLKMMEAMEEVF 63
           +LPV+ FS  NL+ G  KW      V +ALE+YGCF A  D +S+E +   + EAME++F
Sbjct: 13  QLPVIDFSDQNLKPGSSKWDEVTADVLKALEDYGCFEASFDKLSVELNR-SVFEAMEDLF 72

Query: 64  EVPLERKVQNVSEKPFHGYFGQNPLMPIHESMGIEDPLLPSNIQSFSNLMWPSRPNPL-- 123
           E+P+  K +NVS KPFHGY   N    ++ES+GI D  +   +  F+  +WP   N    
Sbjct: 73  ELPIPTKQRNVSSKPFHGYLCHN----LYESLGINDANVLEKVNDFTQQLWPDHGNKSIS 132

Query: 124 ENLKSYAELVSQLEQTVKKMVFESYGLKSLFLSHTQSTKYLLRMIKYRVPHRN------E 183
           E +  ++E + +L+  V++M+ ES+G+++    H  ST YL R++KY  P  +      E
Sbjct: 133 ETIHLFSEQLVELDLMVRRMIMESFGIENYIDEHLNSTYYLTRLMKYTSPPDDDDDDDEE 192

Query: 184 MNLGAFPHTDKSFLTILHQNSVNGLHIKTRDNRWVEYQPPSAESSAKSFIIMAGDAFFAW 243
             LG   HTDK+ +TILHQ  V+GL +KT+D++W++ +P     S  S ++M GD+  A 
Sbjct: 193 TKLGLRSHTDKNIITILHQYQVDGLEVKTKDDKWIKVKP-----SQDSVLVMVGDSLCAL 252

Query: 244 SNGRIYSAPHRVTMSGNRERYSVGLFSF--NDGIVQIPKELVDDKHPQQFKAFDHQDYLR 303
            NGR++S  HRV M+G + RYS GLFS      I+  P+ELVD +HP+ FK F++ D+L 
Sbjct: 253 LNGRLHSPYHRVIMTGKKTRYSTGLFSIPKTGVIIDSPEELVDKEHPRIFKPFEYTDFLH 312

Query: 304 FYSTEKGQSSPSAIKAY 311
           F+ TE G+ + SA+ A+
Sbjct: 313 FFQTEAGRIAQSALHAF 319

BLAST of CmoCh13G009720 vs. Swiss-Prot
Match: AOP1V_ARATH (Probable 2-oxoglutarate-dependent dioxygenase AOP1 (Fragment) OS=Arabidopsis thaliana GN=AOP1 PE=2 SV=1)

HSP 1 Score: 236.5 bits (602), Expect = 4.1e-61
Identity = 124/306 (40.52%), Postives = 186/306 (60.78%), Query Frame = 1

Query: 4   ELPVVRFSSHNLQSGGRKWGSTREKVREALEEYGCFVALCDSVSMEASSLKMMEAMEEVF 63
           +LPV+ FS  NL+ G  KW      V +ALE+YGCF A  D +S+E +   + EAME++F
Sbjct: 6   QLPVIDFSDQNLKPGSSKWDEVTADVLKALEDYGCFEASFDKLSVELNR-SVFEAMEDLF 65

Query: 64  EVPLERKVQNVSEKPFHGYFGQNPLMPIHESMGIEDPLLPSNIQSFSNLMWPSRPNPL-- 123
           E+P+  K +NVS K FHGY   N    ++ES+GI D  +   +  F+  +WP   N    
Sbjct: 66  ELPIPTKQRNVSSKLFHGYLCHN----LYESLGINDANVLEKVNDFTQQLWPDHGNKSIS 125

Query: 124 ENLKSYAELVSQLEQTVKKMVFESYGLKSLFLSHTQSTKYLLRMIKYRVPHRN----EMN 183
           E +  ++E + +L+  V++M+ ES+G++     H  ST YL R++KY  P  +    E  
Sbjct: 126 ETIHLFSEQLVELDLMVRRMIMESFGIEKYIDEHLNSTYYLTRLMKYTSPPDDDDDEETK 185

Query: 184 LGAFPHTDKSFLTILHQNSVNGLHIKTRDNRWVEYQPPSAESSAKSFIIMAGDAFFAWSN 243
           LG   HTDK+ +TILHQ  V+GL +KT+D++W++ +P     S  S ++M GD+  A  N
Sbjct: 186 LGLRSHTDKNIITILHQYQVDGLEVKTKDDKWIKVKP-----SQDSVLVMVGDSLCALLN 245

Query: 244 GRIYSAPHRVTMSGNRERYSVGLFSF--NDGIVQIPKELVDDKHPQQFKAFDHQDYLRFY 302
           GR++S  HRV M+G + RYS GLFS      I+  P+ELVD +HP+ FK F++ D+L F+
Sbjct: 246 GRLHSPYHRVIMTGKKTRYSTGLFSIPKTGVIIDSPEELVDKEHPRIFKPFEYTDFLHFF 301

BLAST of CmoCh13G009720 vs. Swiss-Prot
Match: AOP3L_ARATH (2-oxoglutarate-dependent dioxygenase AOP3 OS=Arabidopsis thaliana GN=AOP3 PE=2 SV=1)

HSP 1 Score: 128.3 bits (321), Expect = 1.6e-28
Identity = 66/182 (36.26%), Postives = 103/182 (56.59%), Query Frame = 1

Query: 4   ELPVVRFSSHNLQSGGRKWGSTREKVREALEEYGCFVALCDSVSMEASSLKMMEAMEEVF 63
           +LP++  S   L+ G  KW   R  VR+ALE+YGCF A  D VSME     +++AM+E+F
Sbjct: 7   QLPLICLSDQTLKPGSSKWVKVRSDVRKALEDYGCFEAKIDQVSMELQG-SVLKAMQELF 66

Query: 64  EVPLERKVQNVSEKPFHGYFGQNPLMPIHESMGIEDPLLPSNIQSFSNLMWPSRPNPLEN 123
            +P E K +NV  KPF GYF  N L    ES GI+D  +      F+  +WP     ++ 
Sbjct: 67  ALPTEAKQRNVCPKPFAGYFSHNGLS---ESFGIKDANILEKAHEFTQQLWPEGNKSIKM 126

Query: 124 LKSYAELVSQLEQTVKKMVFESYGLKSLFLSHTQSTKYLLRMIKYRVPHRNEMNLGAFPH 183
           ++ YAE +++L+  V++++ ESYG++     H  ST Y +R++KY     N++      +
Sbjct: 127 IQLYAEKLAELDMMVRRLILESYGIEYFIDEHLNSTYYRMRLMKYIARPDNDITAAVGAN 184

Query: 184 TD 186
            D
Sbjct: 187 VD 184

BLAST of CmoCh13G009720 vs. Swiss-Prot
Match: AOP3V_ARATH (2-oxoglutarate-dependent dioxygenase AOP3 OS=Arabidopsis thaliana GN=AOP3 PE=2 SV=1)

HSP 1 Score: 128.3 bits (321), Expect = 1.6e-28
Identity = 66/182 (36.26%), Postives = 103/182 (56.59%), Query Frame = 1

Query: 4   ELPVVRFSSHNLQSGGRKWGSTREKVREALEEYGCFVALCDSVSMEASSLKMMEAMEEVF 63
           +LP++  S   L+ G  KW   R  VR+ALE+YGCF A  D VSME     +++AM+E+F
Sbjct: 7   QLPLICLSDQTLKPGSSKWVKVRSDVRKALEDYGCFEAKIDQVSMELQG-SVLKAMQELF 66

Query: 64  EVPLERKVQNVSEKPFHGYFGQNPLMPIHESMGIEDPLLPSNIQSFSNLMWPSRPNPLEN 123
            +P E K +NV  KPF GYF  N L    ES GI+D  +      F+  +WP     ++ 
Sbjct: 67  ALPTEAKQRNVCPKPFAGYFSHNGLS---ESFGIKDANILEKAHEFTQQLWPEGNKSIKM 126

Query: 124 LKSYAELVSQLEQTVKKMVFESYGLKSLFLSHTQSTKYLLRMIKYRVPHRNEMNLGAFPH 183
           ++ YAE +++L+  V++++ ESYG++     H  ST Y +R++KY     N++      +
Sbjct: 127 IQLYAEKLAELDMMVRRLILESYGIEYFIDEHLNSTYYRMRLMKYIARPDNDITAAVGAN 184

Query: 184 TD 186
            D
Sbjct: 187 VD 184

BLAST of CmoCh13G009720 vs. TrEMBL
Match: A5AJI0_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_028513 PE=3 SV=1)

HSP 1 Score: 337.8 bits (865), Expect = 1.5e-89
Identity = 164/313 (52.40%), Postives = 220/313 (70.29%), Query Frame = 1

Query: 2   AGELPVVRFSSHNLQSGGRKWGSTREKVREALEEYGCFVALCDSVSMEASSLKMMEAMEE 61
           A  LPV+ FS+  L+ G R W    + V  ALEEYGCFVA+ D VS E  +    + +EE
Sbjct: 5   APRLPVLNFSTGTLKPGTRSWLLACKDVLHALEEYGCFVAVYDKVSSELDNTTFCQ-LEE 64

Query: 62  VFEVPLERKVQNVSEKPFHGYFGQNPLMPIHESMGIEDPLLPSNIQSFSNLMWPSRPNPL 121
           +F++P E KV+N S+KP+ GY GQ+PL+P+HESMGIED      ++SF+N+MWP+  +  
Sbjct: 65  LFDLPTETKVKNTSDKPYFGYIGQHPLIPLHESMGIEDATTLKGVESFTNIMWPAGNDHF 124

Query: 122 -ENLKSYAELVSQLEQTVKKMVFESYGLKSLFLSHTQSTKYLLRMIKYRVPHRNEMNLGA 181
            E + SY++LVS+LEQ VKKMVFESY  +  + SH QST YLLR+IKYR P +NE  +G 
Sbjct: 125 CETVLSYSKLVSELEQMVKKMVFESYDAEKYYDSHIQSTTYLLRLIKYRGPQKNETCIGT 184

Query: 182 FPHTDKSFLTILHQNSVNGLHIKTRDNRWVEYQPPSAESSAKSFIIMAGDAFFAWSNGRI 241
             HTDKSF+T+LHQN V GL I+T+D  W+ ++P     S  S+++MAGDA  AWSNGRI
Sbjct: 185 SCHTDKSFITVLHQNEVCGLEIRTKDGEWISFKP-----SPSSYVVMAGDALLAWSNGRI 244

Query: 242 YSAPHRVTMSGNRERYSVGLFSFNDGIVQIPKELVDDKHPQQFKAFDHQDYLRFYSTEKG 301
           +S  HRV M+GN+ RYS+GLF+++ GI++IP+EL D++HP QFK F+H   L FY T K 
Sbjct: 245 HSPEHRVIMNGNKARYSIGLFAYHRGIIEIPEELADEEHPLQFKPFNHYGLLHFYFTNKA 304

Query: 302 QSSPSAIKAYCGV 314
             + S  KAYCGV
Sbjct: 305 GQTESTAKAYCGV 311

BLAST of CmoCh13G009720 vs. TrEMBL
Match: D7U0Q7_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_09s0002g05290 PE=3 SV=1)

HSP 1 Score: 337.8 bits (865), Expect = 1.5e-89
Identity = 164/313 (52.40%), Postives = 220/313 (70.29%), Query Frame = 1

Query: 2   AGELPVVRFSSHNLQSGGRKWGSTREKVREALEEYGCFVALCDSVSMEASSLKMMEAMEE 61
           A  LPV+ FS+  L+ G R W    + V  ALEEYGCFVA+ D VS E  +    + +EE
Sbjct: 5   APRLPVLNFSTGTLKPGTRSWLLACKDVLHALEEYGCFVAVYDKVSSELDNTTFCQ-LEE 64

Query: 62  VFEVPLERKVQNVSEKPFHGYFGQNPLMPIHESMGIEDPLLPSNIQSFSNLMWPSRPNPL 121
           +F++P E KV+N S+KP+ GY GQ+PL+P+HESMGIED      ++SF+N+MWP+  +  
Sbjct: 65  LFDLPTETKVKNTSDKPYFGYIGQHPLIPLHESMGIEDATTLKGVESFTNIMWPAGNDHF 124

Query: 122 -ENLKSYAELVSQLEQTVKKMVFESYGLKSLFLSHTQSTKYLLRMIKYRVPHRNEMNLGA 181
            E + SY++LVS+LEQ VKKMVFESY  +  + SH QST YLLR+IKYR P +NE  +G 
Sbjct: 125 CETVLSYSKLVSELEQMVKKMVFESYDAEKYYDSHIQSTTYLLRLIKYRGPQKNETCIGT 184

Query: 182 FPHTDKSFLTILHQNSVNGLHIKTRDNRWVEYQPPSAESSAKSFIIMAGDAFFAWSNGRI 241
             HTDKSF+T+LHQN V GL I+T+D  W+ ++P     S  S+++MAGDA  AWSNGRI
Sbjct: 185 SCHTDKSFITVLHQNEVCGLEIRTKDGEWISFKP-----SPSSYVVMAGDALLAWSNGRI 244

Query: 242 YSAPHRVTMSGNRERYSVGLFSFNDGIVQIPKELVDDKHPQQFKAFDHQDYLRFYSTEKG 301
           +S  HRV M+GN+ RYS+GLF+++ GI++IP+EL D++HP QFK F+H   L FY T K 
Sbjct: 245 HSPEHRVIMNGNKARYSIGLFAYHRGIIEIPEELADEEHPLQFKPFNHYGLLHFYFTNKA 304

Query: 302 QSSPSAIKAYCGV 314
             + S  KAYCGV
Sbjct: 305 GQAESTAKAYCGV 311

BLAST of CmoCh13G009720 vs. TrEMBL
Match: V4RZ66_9ROSI (Uncharacterized protein OS=Citrus clementina GN=CICLE_v10027501mg PE=3 SV=1)

HSP 1 Score: 331.6 bits (849), Expect = 1.1e-87
Identity = 166/313 (53.04%), Postives = 221/313 (70.61%), Query Frame = 1

Query: 4   ELPVVRFSSHNLQSGGRKWGSTREKVREALEEYGCFVALCDSVSMEASSLKMMEAMEEVF 63
           +LPVV  S  N + G   W  T  KVREALEE+GCFVAL D VS+E  +  +  A EE+F
Sbjct: 31  KLPVVNLSKENFKPGASSWLGTSRKVREALEEFGCFVALYDEVSLELHNA-IFSAAEELF 90

Query: 64  EVPLERKVQNVSEKPFHGYFGQNPLMP-IHESMGIEDPLLPSNIQSFSNLMWPS-RPNPL 123
            +P+E K +NVSEKP++GY G NPL+P I+E MG++        Q+F+NL+WP    N  
Sbjct: 91  NLPIEIKEKNVSEKPYYGYLGNNPLVPAIYEGMGVDYANTIEGTQNFTNLIWPEGNENFC 150

Query: 124 ENLKSYAELVSQLEQTVKKMVFESYGLKSLFLSHTQSTKYLLRMIKYRVPHRNEMNLGAF 183
           + + SY+ LVS+LEQ VK+MVFESYG+   + S  +S+ YLLR++KYR P  NE NLG  
Sbjct: 151 KTVVSYSRLVSELEQMVKRMVFESYGVDKYYDSVLESSTYLLRIMKYRCPETNEKNLGCD 210

Query: 184 PHTDKSFLTILHQNSVNGLHIKTRDNRWVEYQPPSAESSAKSFIIMAGDAFFAWSNGRIY 243
            HTDKSF+T+LHQN VNGL I+T+D  W+ +  P    +  SFI++AGDAF AWSN RI+
Sbjct: 211 VHTDKSFITVLHQNEVNGLEIRTKDGCWIGFDDP----TPSSFIVLAGDAFLAWSNSRIH 270

Query: 244 SAPHRVTMSGNRE-RYSVGLFSFNDGIVQIPKELVDDKHPQQFKAFDHQDYLRFYSTEKG 303
           S  HRV ++G ++ RYS+GLF+FNDGI+QIP+ELVDD+HP +FK+F+H   LR+Y TE+G
Sbjct: 271 SPVHRVILNGIKQARYSLGLFTFNDGIIQIPEELVDDEHPLKFKSFEHFGLLRYYLTEEG 330

Query: 304 QSSPSAIKAYCGV 314
           Q +    KAYC V
Sbjct: 331 QKAECTAKAYCSV 338

BLAST of CmoCh13G009720 vs. TrEMBL
Match: A0A0A0LT96_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_1G044830 PE=4 SV=1)

HSP 1 Score: 327.4 bits (838), Expect = 2.0e-86
Identity = 165/236 (69.92%), Postives = 195/236 (82.63%), Query Frame = 1

Query: 4   ELPVVRFSSHNLQSGGRKWGSTREKVREALEEYGCFVALCDSVSMEASSLKMMEAMEEVF 63
           +LPVV+F S NL+SG  +W ST +KVREALEEYGCFVAL D+VS E SS  M ++++E+F
Sbjct: 9   QLPVVKFCSQNLKSGTEEWVSTTKKVREALEEYGCFVALYDTVSAEVSS-NMFDSLKELF 68

Query: 64  -EVPLERKVQNVSEKPFHGYFGQNPLMPIHESMGIEDPLLPSNIQSFSNLMWPSRPNP-- 123
            EVPLERK+QNVSEKP+HGYFGQNPLMPIHESMGIE P+LP+NI SF+NLMWPSR N   
Sbjct: 69  DEVPLERKLQNVSEKPYHGYFGQNPLMPIHESMGIEHPILPTNINSFTNLMWPSRGNHSF 128

Query: 124 LENLKSYAELVSQLEQTVKKMVFESYGLKSLFLSHTQSTKYLLRMIKYRVPHRNEMNLGA 183
            EN+ +YA LVS+L++ VK MVFESYG+ +   SH +STKYL+RMIKYRVP   EMNLGA
Sbjct: 129 RENVTAYANLVSELDKRVKSMVFESYGVGNALRSHMESTKYLMRMIKYRVPKEKEMNLGA 188

Query: 184 FPHTDKSFLTILHQNSVNGLHIKTRDNRWVEYQPPSAESSAKSFIIMAGDAFFAWS 237
           FPHTDKSFLTILHQN VNGL IKTRDN+W++Y P S+ SS  SFI+MAGDAFF  S
Sbjct: 189 FPHTDKSFLTILHQNEVNGLQIKTRDNKWIQYHPFSS-SSTSSFIVMAGDAFFVSS 242

BLAST of CmoCh13G009720 vs. TrEMBL
Match: W9STP2_9ROSA (Gibberellin 3-beta-dioxygenase 4 OS=Morus notabilis GN=L484_019467 PE=3 SV=1)

HSP 1 Score: 323.9 bits (829), Expect = 2.2e-85
Identity = 157/313 (50.16%), Postives = 217/313 (69.33%), Query Frame = 1

Query: 4   ELPVVRFSSHNLQSGGRKWGSTREKVREALEEYGCFVALCDSVSMEASSLKMMEAMEEVF 63
           ++P + FS  NL  G   W S    VR ALEEYGCFVAL D +S +  +  M   ++E+F
Sbjct: 8   KIPTIDFSEGNLNPGTSSWFSVCNDVRRALEEYGCFVALYDKISQQLRT-SMFSELKELF 67

Query: 64  EVPLERKVQNVSEKPFHGYFGQNP-LMPIHESMGIEDPLLPSNIQSFSNLMWPSRPNP-- 123
           ++P E K++NVS+KP+ GY G +P + P+HESMGIE+       +SF+N++WP   N   
Sbjct: 68  DLPTEIKMKNVSDKPYFGYIGNHPSIPPLHESMGIENATTLEGGESFTNIIWPKGGNNHF 127

Query: 124 LENLKSYAELVSQLEQTVKKMVFESYGLKSLFLSHTQSTKYLLRMIKYRVPHRNEMNLGA 183
            E + SY+ LVS+LE+ VK+MVF+SYG++  + SH  ST +LL++IKYRVP  NE ++G 
Sbjct: 128 CETVVSYSRLVSELEKMVKRMVFQSYGVEKYYDSHLGSTTFLLKLIKYRVPEMNESSIGC 187

Query: 184 FPHTDKSFLTILHQNSVNGLHIKTRDNRWVEYQPPSAESSAKSFIIMAGDAFFAWSNGRI 243
            PHTDKSF+T+LHQN VNGL I+TRD  W++++P     S  SF+++AGDAF AW+NGRI
Sbjct: 188 APHTDKSFITVLHQNEVNGLEIETRDGDWIQFEP-----SPSSFLVLAGDAFLAWTNGRI 247

Query: 244 YSAPHRVTMSGNRERYSVGLFSFNDGIVQIPKELVDDKHPQQFKAFDHQDYLRFYSTEKG 303
           +SA HRV M+G + RYS+GLFS+++G ++IP ELVD++HPQQFK+FDH   L  Y     
Sbjct: 248 HSAAHRVVMTGTKPRYSIGLFSYHNGTIEIPDELVDEQHPQQFKSFDHYGLLHHY--HAA 307

Query: 304 QSSPSAIKAYCGV 314
               S  K YCGV
Sbjct: 308 PKIESTAKDYCGV 312

BLAST of CmoCh13G009720 vs. TAIR10
Match: AT1G52820.1 (AT1G52820.1 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein)

HSP 1 Score: 280.0 bits (715), Expect = 1.8e-75
Identity = 136/312 (43.59%), Postives = 206/312 (66.03%), Query Frame = 1

Query: 5   LPVVRFSSHNLQSGGRKWGSTREKVREALEEYGCFVALCDSVSMEASSLKMMEAMEEVFE 64
           LPV+ FS+ NL+ G  +W  TR  V++AL++YG F A  D +  E     +  A+EE+F+
Sbjct: 12  LPVIDFSNKNLKPGEPEWDLTRADVQKALQDYGYFEASFDRIPFELRK-SVFGALEELFD 71

Query: 65  VPLERKVQNVSEKPFHGYFGQNPLMPIHESMGIEDPLLPSNIQSFSNLMWPSRPNPLEN- 124
           +PL+ K++NVS+KPFHGY GQ P++P++ESMGI+D  +   + +F+  +WP         
Sbjct: 72  LPLQTKLRNVSKKPFHGYVGQYPMVPLYESMGIDDSDIAEKVDAFTEKLWPQGNISFSTT 131

Query: 125 LKSYAELVSQLEQTVKKMVFESYGLKSLFLSHTQSTKYLLRMIKYRVPHRNEMNLGAFPH 184
           ++S+++ +S+L+ T+++M+ ES+GL      H  ST YLLR++KY+ P   E  +G   H
Sbjct: 132 IQSFSKKLSELDITIRRMIMESFGLDKYIDEHLHSTNYLLRVMKYKGPDTEETKVGLNAH 191

Query: 185 TDKSFLTILHQNSVNGLHIKTRDNRWVEYQPPSAESSAKSFIIMAGDAFFAWSNGRIYSA 244
           TDK+ +TIL+QN V GL ++T+D  W++ +P     +  SF +M GD+ +A  NGR++S 
Sbjct: 192 TDKNIVTILYQNHVEGLEVQTKDKNWIKVKP-----TQDSFTVMIGDSLYALLNGRLHSP 251

Query: 245 PHRVTMSGNRERYSVGLFSFNDG--IVQIPKELVDDKHPQQFKAFDHQDYLRFYSTEKGQ 304
            HRV M+G   RYS+GLFS      IV  P ELVD++HP+ FK FDH ++L+FY TE GQ
Sbjct: 252 YHRVMMTGTETRYSLGLFSIPKAGHIVSSPDELVDEEHPRLFKPFDHVEFLQFYYTEAGQ 311

Query: 305 SSPSAIKAYCGV 314
            S SA+K YCG+
Sbjct: 312 RSQSALKTYCGI 317

BLAST of CmoCh13G009720 vs. TAIR10
Match: AT1G52790.1 (AT1G52790.1 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein)

HSP 1 Score: 276.2 bits (705), Expect = 2.7e-74
Identity = 135/314 (42.99%), Postives = 204/314 (64.97%), Query Frame = 1

Query: 1   MAGELPVVRFSSHNLQSGGRKWGSTREKVREALEEYGCFVA-LCDSVSMEASSLKMMEAM 60
           M+ ++P + FS  +L+ G + W STRE +R+ALEEYGCF+  L D   ++    ++  ++
Sbjct: 1   MSPKIPTLDFSREDLKPGTKYWESTRENIRQALEEYGCFIIDLKDKTPLDLLD-RVFGSL 60

Query: 61  EEVFEVPLERKVQNVSEKPFHGYFGQNPLMPIHESMGIEDPLLPSNIQSFSNLMWPSRPN 120
            ++F++P + K++N  +KP +GY GQ P +P+HES+GI++       +SF+ LMWP    
Sbjct: 61  VDLFDLPTQTKMKNKYDKPLNGYVGQIPALPLHESLGIDNATSLEATRSFTGLMWPQGNE 120

Query: 121 PL-ENLKSYAELVSQLEQTVKKMVFESYGLKSLFLSHTQSTKYLLRMIKYRVPHRNEMNL 180
              E L  YAE  ++L+Q V +MVF+SY ++  +  + +ST YLLR++K R P+     L
Sbjct: 121 HFSECLYKYAEFAAELDQMVTRMVFQSYNVEKYYDPYIESTTYLLRVLKNRAPNNENPTL 180

Query: 181 GAFPHTDKSFLTILHQNSVNGLHIKTRDNRWVEYQPPSAESSAKSFIIMAGDAFFAWSNG 240
           G   HTDKSF TILHQ+ VNGL ++TR+   +        SS   F+++AGDA  AWSN 
Sbjct: 181 GFVTHTDKSFTTILHQDQVNGLEMETREGERININL----SSPSLFMVVAGDALMAWSND 240

Query: 241 RIYSAPHRVTMSGNRERYSVGLFSFNDGIVQIPKELVDDKHPQQFKAFDHQDYLRFYSTE 300
           R++S  H+V +SG  +RYS+G+F+FN+G +Q+P+EL+D +HP  +K FDH   LRFY T+
Sbjct: 241 RVWSPRHQVLVSGETDRYSLGMFAFNNGTLQVPEELIDHQHPLMYKPFDHLGLLRFYRTD 300

Query: 301 KGQSSPSAIKAYCG 313
            G  S   IKAYCG
Sbjct: 301 TGYKSECPIKAYCG 309

BLAST of CmoCh13G009720 vs. TAIR10
Match: AT1G52800.1 (AT1G52800.1 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein)

HSP 1 Score: 274.2 bits (700), Expect = 1.0e-73
Identity = 134/305 (43.93%), Postives = 201/305 (65.90%), Query Frame = 1

Query: 11  SSHNLQSGGRKWGSTREKVREALEEYGCFVALCDSVSMEASSLKMMEAMEEVFEVPLERK 70
           S   L+     W S   +  EALEEYGCF+A+ D V+ +     +  A EE+F++P E K
Sbjct: 17  SQQELKPNTSTWRSVSREACEALEEYGCFLAVYDGVTQQLDD-SIFAAAEELFDLPTETK 76

Query: 71  VQNVSEKPFHGYFGQNPLMPIHESMGIEDPLLPSNIQSFSNLMWPSRPNPLEN-LKSYAE 130
            +NV+EKP+HGY GQ P++P+HE +G++        Q F++LMWP   +   N + +++ 
Sbjct: 77  KKNVNEKPYHGYVGQMPVIPLHEGLGVDYVTNKEIAQRFTHLMWPQGNDRFCNTVHTFSN 136

Query: 131 LVSQLEQTVKKMVFESYGLKSLFLSHTQSTKYLLRMIKYRVPHRNEMNLGAFP-HTDKSF 190
            V++L++ V +M+FE+YG++  + SH  S  YLL+ +KY  P  + +++ AFP HTDK+F
Sbjct: 137 AVAELDRLVVRMIFENYGVEKHYESHVGSKTYLLKFLKYLAPPES-ISMPAFPQHTDKTF 196

Query: 191 LTILHQNSVNGLHIKTRDNRWVEYQPPSAESSAKSFIIMAGDAFFAWSNGRIYSAPHRVT 250
           L+ILHQN VNGL +K++D  W+  Q P      KS+++MAGD    WSN RI S  HRVT
Sbjct: 197 LSILHQNDVNGLEVKSKDGEWISLQLPP-----KSYVVMAGDISMGWSNDRIRSCEHRVT 256

Query: 251 MSGNRERYSVGLFSFNDGIVQIPKELVDDKHPQQFKAFDHQDYLRFYSTEKGQSSPSAIK 310
           M G++ RY++GLFSF   +V IP+ELVDDKHP  +K FD+   + FY+T++G+ + S +K
Sbjct: 257 MEGDKTRYTLGLFSFLTDLVSIPEELVDDKHPLMYKPFDNIALINFYTTKEGREANSTLK 314

Query: 311 AYCGV 314
           AYCG+
Sbjct: 317 AYCGI 314

BLAST of CmoCh13G009720 vs. TAIR10
Match: AT4G03070.1 (AT4G03070.1 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein)

HSP 1 Score: 245.7 bits (626), Expect = 3.8e-65
Identity = 128/317 (40.38%), Postives = 194/317 (61.20%), Query Frame = 1

Query: 4   ELPVVRFSSHNLQSGGRKWGSTREKVREALEEYGCFVALCDSVSMEASSLKMMEAMEEVF 63
           +LPV+ FS  NL+ G  KW      V +ALE+YGCF A  D +S+E +   + EAME++F
Sbjct: 13  QLPVIDFSDQNLKPGSSKWDEVTADVLKALEDYGCFEASFDKLSVELNR-SVFEAMEDLF 72

Query: 64  EVPLERKVQNVSEKPFHGYFGQNPLMPIHESMGIEDPLLPSNIQSFSNLMWPSRPNPL-- 123
           E+P+  K +NVS KPFHGY   N    ++ES+GI D  +   +  F+  +WP   N    
Sbjct: 73  ELPIPTKQRNVSSKPFHGYLCHN----LYESLGINDANVLEKVNDFTQQLWPDHGNKSIS 132

Query: 124 ENLKSYAELVSQLEQTVKKMVFESYGLKSLFLSHTQSTKYLLRMIKYRVPHRN------E 183
           E +  ++E + +L+  V++M+ ES+G+++    H  ST YL R++KY  P  +      E
Sbjct: 133 ETIHLFSEQLVELDLMVRRMIMESFGIENYIDEHLNSTYYLTRLMKYTSPPDDDDDDDEE 192

Query: 184 MNLGAFPHTDKSFLTILHQNSVNGLHIKTRDNRWVEYQPPSAESSAKSFIIMAGDAFFAW 243
             LG   HTDK+ +TILHQ  V+GL +KT+D++W++ +P     S  S ++M GD+  A 
Sbjct: 193 TKLGLRSHTDKNIITILHQYQVDGLEVKTKDDKWIKVKP-----SQDSVLVMVGDSLCAL 252

Query: 244 SNGRIYSAPHRVTMSGNRERYSVGLFSF--NDGIVQIPKELVDDKHPQQFKAFDHQDYLR 303
            NGR++S  HRV M+G + RYS GLFS      I+  P+ELVD +HP+ FK F++ D+L 
Sbjct: 253 LNGRLHSPYHRVIMTGKKTRYSTGLFSIPKTGVIIDSPEELVDKEHPRIFKPFEYTDFLH 312

Query: 304 FYSTEKGQSSPSAIKAY 311
           F+ TE G+ + SA+ A+
Sbjct: 313 FFQTEAGRIAQSALHAF 319

BLAST of CmoCh13G009720 vs. TAIR10
Match: AT1G80320.1 (AT1G80320.1 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein)

HSP 1 Score: 233.4 bits (594), Expect = 2.0e-61
Identity = 127/316 (40.19%), Postives = 187/316 (59.18%), Query Frame = 1

Query: 5   LPVVRFSSHNLQSGGRKWGSTREKVREALEEYGCFVALCDSVSMEASSLKMMEAMEEVFE 64
           LP++ FSS  L  G   W +TR+ VR A+E  G FVA    VS +     ++  M+E++ 
Sbjct: 9   LPILDFSSDKLVRGTSHWITTRDDVRRAMEGQGWFVAEFSGVSSDLRD-NLLAGMKEMYY 68

Query: 65  VPLERKVQNVSEKPFHGYFGQN-PLMPIHESMGIEDPLLPSNIQSFSNLMWPSRPNPL-E 124
           +P + K++N + K  HGY         IHES+GI+        + FS L+WP   +P  +
Sbjct: 69  LPDQIKIKNENHKASHGYMSMVVDDYRIHESLGIDYATELQACKDFSKLLWPQGNDPFCQ 128

Query: 125 NLKSYAELVSQLEQTVKKMVFESYGL--KSLFLSHTQSTKYLLRMIKYRVPHRNEMNLGA 184
               YA  +++L+QTV +M++ESYG+  K   +SH++ST+YLLRM+ YR     E N G 
Sbjct: 129 TTHMYAMTMAELDQTVMRMLYESYGMDEKKHSVSHSESTRYLLRMLSYRRQQNGEANTGF 188

Query: 185 FPHTDKSFLTILHQNSVNGLHIKTRDNRWVEYQPPSAESSAKSFIIMAGDAFFAWSNGRI 244
             HTDKSF++ILHQN V GL +KT   +WV + P     S   F++++G    AWSN RI
Sbjct: 189 VSHTDKSFMSILHQNHVGGLQLKTMTGQWVGFNP-----SPTRFVVLSGMGLTAWSNDRI 248

Query: 245 YSAPHRVTMSGNRERYSVGLFSFNDGIVQIPKELVDDKHPQQFKAFDHQDYLRFYST--- 304
            +  H+V MS +  RYS+G FSF+ G ++ P+ELVDD+HP ++  F+H   LRFY +   
Sbjct: 249 KACYHKVVMSADEIRYSLGFFSFHKGTIRTPEELVDDQHPLRYNPFEHDGLLRFYESYIN 308

Query: 305 EKGQSSPSAIKAYCGV 314
              +SS   ++ YCGV
Sbjct: 309 SLKKSSEDLLQIYCGV 318

BLAST of CmoCh13G009720 vs. NCBI nr
Match: gi|778664781|ref|XP_011660352.1| (PREDICTED: probable 2-oxoglutarate-dependent dioxygenase AOP1 [Cucumis sativus])

HSP 1 Score: 488.4 bits (1256), Expect = 9.6e-135
Identity = 240/326 (73.62%), Postives = 277/326 (84.97%), Query Frame = 1

Query: 4   ELPVVRFSSHNLQSGGRKWGSTREKVREALEEYGCFVALCDSVSMEASSLKMMEAMEEVF 63
           +LPVV+F S NL+SG  +W ST +KVREALEEYGCFVAL D+VS E SS  M ++++E+F
Sbjct: 9   QLPVVKFCSQNLKSGTEEWVSTTKKVREALEEYGCFVALYDTVSAEVSS-NMFDSLKELF 68

Query: 64  -EVPLERKVQNVSEKPFHGYFGQNPLMPIHESMGIEDPLLPSNIQSFSNLMWPSRPNPL- 123
            EVPLERK+QNVSEKP+HGYFGQNPLMPIHESMGIE P+LP+NI SF+NLMWPSR N   
Sbjct: 69  DEVPLERKLQNVSEKPYHGYFGQNPLMPIHESMGIEHPILPTNINSFTNLMWPSRGNHSF 128

Query: 124 -ENLKSYAELVSQLEQTVKKMVFESYGLKSLFLSHTQSTKYLLRMIKYRVPHRNEMNLGA 183
            EN+ +YA LVS+L++ VK MVFESYG+ +   SH +STKYL+RMIKYRVP   EMNLGA
Sbjct: 129 RENVTAYANLVSELDKRVKSMVFESYGVGNALRSHMESTKYLMRMIKYRVPKEKEMNLGA 188

Query: 184 FPHTDKSFLTILHQNSVNGLHIKTRDNRWVEYQPPSAESSAKSFIIMAGDAFFAWSNGRI 243
           FPHTDKSFLTILHQN VNGL IKTRDN+W++Y P S+ SS  SFI+MAGDAFFAWSNGRI
Sbjct: 189 FPHTDKSFLTILHQNEVNGLQIKTRDNKWIQYHPFSS-SSTSSFIVMAGDAFFAWSNGRI 248

Query: 244 YSAPHRVTMSGNRERYSVGLFSFNDGIVQIPKELVDDKHPQQFKAFDHQDYLRFYSTEKG 303
           YS PHRV MSGN+ERYSVGLFSFN+GIVQIPKELVDDKHPQ FK FDHQDYLRFYSTE+G
Sbjct: 249 YSPPHRVIMSGNKERYSVGLFSFNNGIVQIPKELVDDKHPQLFKPFDHQDYLRFYSTERG 308

Query: 304 QSSPSAIKAYCGVANGLLDSRTCIPM 327
           Q+SP AIKAYCGV N L+D+ T  P+
Sbjct: 309 QNSPCAIKAYCGVGNSLIDTDTRSPL 332

BLAST of CmoCh13G009720 vs. NCBI nr
Match: gi|778657573|ref|XP_004151475.2| (PREDICTED: probable 2-oxoglutarate-dependent dioxygenase AOP1.2 [Cucumis sativus])

HSP 1 Score: 426.0 bits (1094), Expect = 5.9e-116
Identity = 206/275 (74.91%), Postives = 237/275 (86.18%), Query Frame = 1

Query: 55  MMEAMEEVF-EVPLERKVQNVSEKPFHGYFGQNPLMPIHESMGIEDPLLPSNIQSFSNLM 114
           M ++++E+F EVPLERK+QNVSEKP+HGYFGQNPLMPIHESMGIE P+LP+NI SF+NLM
Sbjct: 1   MFDSLKELFDEVPLERKLQNVSEKPYHGYFGQNPLMPIHESMGIEHPILPTNINSFTNLM 60

Query: 115 WPSRPNPL--ENLKSYAELVSQLEQTVKKMVFESYGLKSLFLSHTQSTKYLLRMIKYRVP 174
           WPSR N    EN+ +YA LVS+L++ VK MVFESYG+ +   SH +STKYL+RMIKYRVP
Sbjct: 61  WPSRGNHSFRENVTAYANLVSELDKRVKSMVFESYGVGNALRSHMESTKYLMRMIKYRVP 120

Query: 175 HRNEMNLGAFPHTDKSFLTILHQNSVNGLHIKTRDNRWVEYQPPSAESSAKSFIIMAGDA 234
              EMNLGAFPHTDKSFLTILHQN VNGL IKTRDN+W++Y P S+ SS  SFI+MAGDA
Sbjct: 121 KEKEMNLGAFPHTDKSFLTILHQNEVNGLQIKTRDNKWIQYHPFSS-SSTSSFIVMAGDA 180

Query: 235 FFAWSNGRIYSAPHRVTMSGNRERYSVGLFSFNDGIVQIPKELVDDKHPQQFKAFDHQDY 294
           FFAWSNGRIYS PHRV MSGN+ERYSVGLFSFN+GIVQIPKELVDDKHPQ FK FDHQDY
Sbjct: 181 FFAWSNGRIYSPPHRVIMSGNKERYSVGLFSFNNGIVQIPKELVDDKHPQLFKPFDHQDY 240

Query: 295 LRFYSTEKGQSSPSAIKAYCGVANGLLDSRTCIPM 327
           LRFYSTE+GQ+SP AIKAYCGV N L+D+ T  P+
Sbjct: 241 LRFYSTERGQNSPCAIKAYCGVGNSLIDTDTRSPL 274

BLAST of CmoCh13G009720 vs. NCBI nr
Match: gi|147852210|emb|CAN80139.1| (hypothetical protein VITISV_028513 [Vitis vinifera])

HSP 1 Score: 337.8 bits (865), Expect = 2.1e-89
Identity = 164/313 (52.40%), Postives = 220/313 (70.29%), Query Frame = 1

Query: 2   AGELPVVRFSSHNLQSGGRKWGSTREKVREALEEYGCFVALCDSVSMEASSLKMMEAMEE 61
           A  LPV+ FS+  L+ G R W    + V  ALEEYGCFVA+ D VS E  +    + +EE
Sbjct: 5   APRLPVLNFSTGTLKPGTRSWLLACKDVLHALEEYGCFVAVYDKVSSELDNTTFCQ-LEE 64

Query: 62  VFEVPLERKVQNVSEKPFHGYFGQNPLMPIHESMGIEDPLLPSNIQSFSNLMWPSRPNPL 121
           +F++P E KV+N S+KP+ GY GQ+PL+P+HESMGIED      ++SF+N+MWP+  +  
Sbjct: 65  LFDLPTETKVKNTSDKPYFGYIGQHPLIPLHESMGIEDATTLKGVESFTNIMWPAGNDHF 124

Query: 122 -ENLKSYAELVSQLEQTVKKMVFESYGLKSLFLSHTQSTKYLLRMIKYRVPHRNEMNLGA 181
            E + SY++LVS+LEQ VKKMVFESY  +  + SH QST YLLR+IKYR P +NE  +G 
Sbjct: 125 CETVLSYSKLVSELEQMVKKMVFESYDAEKYYDSHIQSTTYLLRLIKYRGPQKNETCIGT 184

Query: 182 FPHTDKSFLTILHQNSVNGLHIKTRDNRWVEYQPPSAESSAKSFIIMAGDAFFAWSNGRI 241
             HTDKSF+T+LHQN V GL I+T+D  W+ ++P     S  S+++MAGDA  AWSNGRI
Sbjct: 185 SCHTDKSFITVLHQNEVCGLEIRTKDGEWISFKP-----SPSSYVVMAGDALLAWSNGRI 244

Query: 242 YSAPHRVTMSGNRERYSVGLFSFNDGIVQIPKELVDDKHPQQFKAFDHQDYLRFYSTEKG 301
           +S  HRV M+GN+ RYS+GLF+++ GI++IP+EL D++HP QFK F+H   L FY T K 
Sbjct: 245 HSPEHRVIMNGNKARYSIGLFAYHRGIIEIPEELADEEHPLQFKPFNHYGLLHFYFTNKA 304

Query: 302 QSSPSAIKAYCGV 314
             + S  KAYCGV
Sbjct: 305 GQTESTAKAYCGV 311

BLAST of CmoCh13G009720 vs. NCBI nr
Match: gi|225442749|ref|XP_002284974.1| (PREDICTED: probable 2-oxoglutarate-dependent dioxygenase AOP1 [Vitis vinifera])

HSP 1 Score: 337.8 bits (865), Expect = 2.1e-89
Identity = 164/313 (52.40%), Postives = 220/313 (70.29%), Query Frame = 1

Query: 2   AGELPVVRFSSHNLQSGGRKWGSTREKVREALEEYGCFVALCDSVSMEASSLKMMEAMEE 61
           A  LPV+ FS+  L+ G R W    + V  ALEEYGCFVA+ D VS E  +    + +EE
Sbjct: 5   APRLPVLNFSTGTLKPGTRSWLLACKDVLHALEEYGCFVAVYDKVSSELDNTTFCQ-LEE 64

Query: 62  VFEVPLERKVQNVSEKPFHGYFGQNPLMPIHESMGIEDPLLPSNIQSFSNLMWPSRPNPL 121
           +F++P E KV+N S+KP+ GY GQ+PL+P+HESMGIED      ++SF+N+MWP+  +  
Sbjct: 65  LFDLPTETKVKNTSDKPYFGYIGQHPLIPLHESMGIEDATTLKGVESFTNIMWPAGNDHF 124

Query: 122 -ENLKSYAELVSQLEQTVKKMVFESYGLKSLFLSHTQSTKYLLRMIKYRVPHRNEMNLGA 181
            E + SY++LVS+LEQ VKKMVFESY  +  + SH QST YLLR+IKYR P +NE  +G 
Sbjct: 125 CETVLSYSKLVSELEQMVKKMVFESYDAEKYYDSHIQSTTYLLRLIKYRGPQKNETCIGT 184

Query: 182 FPHTDKSFLTILHQNSVNGLHIKTRDNRWVEYQPPSAESSAKSFIIMAGDAFFAWSNGRI 241
             HTDKSF+T+LHQN V GL I+T+D  W+ ++P     S  S+++MAGDA  AWSNGRI
Sbjct: 185 SCHTDKSFITVLHQNEVCGLEIRTKDGEWISFKP-----SPSSYVVMAGDALLAWSNGRI 244

Query: 242 YSAPHRVTMSGNRERYSVGLFSFNDGIVQIPKELVDDKHPQQFKAFDHQDYLRFYSTEKG 301
           +S  HRV M+GN+ RYS+GLF+++ GI++IP+EL D++HP QFK F+H   L FY T K 
Sbjct: 245 HSPEHRVIMNGNKARYSIGLFAYHRGIIEIPEELADEEHPLQFKPFNHYGLLHFYFTNKA 304

Query: 302 QSSPSAIKAYCGV 314
             + S  KAYCGV
Sbjct: 305 GQAESTAKAYCGV 311

BLAST of CmoCh13G009720 vs. NCBI nr
Match: gi|567869013|ref|XP_006427128.1| (hypothetical protein CICLE_v10027501mg [Citrus clementina])

HSP 1 Score: 331.6 bits (849), Expect = 1.5e-87
Identity = 166/313 (53.04%), Postives = 221/313 (70.61%), Query Frame = 1

Query: 4   ELPVVRFSSHNLQSGGRKWGSTREKVREALEEYGCFVALCDSVSMEASSLKMMEAMEEVF 63
           +LPVV  S  N + G   W  T  KVREALEE+GCFVAL D VS+E  +  +  A EE+F
Sbjct: 31  KLPVVNLSKENFKPGASSWLGTSRKVREALEEFGCFVALYDEVSLELHNA-IFSAAEELF 90

Query: 64  EVPLERKVQNVSEKPFHGYFGQNPLMP-IHESMGIEDPLLPSNIQSFSNLMWPS-RPNPL 123
            +P+E K +NVSEKP++GY G NPL+P I+E MG++        Q+F+NL+WP    N  
Sbjct: 91  NLPIEIKEKNVSEKPYYGYLGNNPLVPAIYEGMGVDYANTIEGTQNFTNLIWPEGNENFC 150

Query: 124 ENLKSYAELVSQLEQTVKKMVFESYGLKSLFLSHTQSTKYLLRMIKYRVPHRNEMNLGAF 183
           + + SY+ LVS+LEQ VK+MVFESYG+   + S  +S+ YLLR++KYR P  NE NLG  
Sbjct: 151 KTVVSYSRLVSELEQMVKRMVFESYGVDKYYDSVLESSTYLLRIMKYRCPETNEKNLGCD 210

Query: 184 PHTDKSFLTILHQNSVNGLHIKTRDNRWVEYQPPSAESSAKSFIIMAGDAFFAWSNGRIY 243
            HTDKSF+T+LHQN VNGL I+T+D  W+ +  P    +  SFI++AGDAF AWSN RI+
Sbjct: 211 VHTDKSFITVLHQNEVNGLEIRTKDGCWIGFDDP----TPSSFIVLAGDAFLAWSNSRIH 270

Query: 244 SAPHRVTMSGNRE-RYSVGLFSFNDGIVQIPKELVDDKHPQQFKAFDHQDYLRFYSTEKG 303
           S  HRV ++G ++ RYS+GLF+FNDGI+QIP+ELVDD+HP +FK+F+H   LR+Y TE+G
Sbjct: 271 SPVHRVILNGIKQARYSLGLFTFNDGIIQIPEELVDDEHPLKFKSFEHFGLLRYYLTEEG 330

Query: 304 QSSPSAIKAYCGV 314
           Q +    KAYC V
Sbjct: 331 QKAECTAKAYCSV 338

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
AOP1L_ARATH8.0e-6540.51Probable 2-oxoglutarate-dependent dioxygenase AOP1.2 OS=Arabidopsis thaliana GN=... [more]
AOP1C_ARATH6.8e-6440.38Probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Arabidopsis thaliana GN=AO... [more]
AOP1V_ARATH4.1e-6140.52Probable 2-oxoglutarate-dependent dioxygenase AOP1 (Fragment) OS=Arabidopsis tha... [more]
AOP3L_ARATH1.6e-2836.262-oxoglutarate-dependent dioxygenase AOP3 OS=Arabidopsis thaliana GN=AOP3 PE=2 S... [more]
AOP3V_ARATH1.6e-2836.262-oxoglutarate-dependent dioxygenase AOP3 OS=Arabidopsis thaliana GN=AOP3 PE=2 S... [more]
Match NameE-valueIdentityDescription
A5AJI0_VITVI1.5e-8952.40Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_028513 PE=3 SV=1[more]
D7U0Q7_VITVI1.5e-8952.40Putative uncharacterized protein OS=Vitis vinifera GN=VIT_09s0002g05290 PE=3 SV=... [more]
V4RZ66_9ROSI1.1e-8753.04Uncharacterized protein OS=Citrus clementina GN=CICLE_v10027501mg PE=3 SV=1[more]
A0A0A0LT96_CUCSA2.0e-8669.92Uncharacterized protein OS=Cucumis sativus GN=Csa_1G044830 PE=4 SV=1[more]
W9STP2_9ROSA2.2e-8550.16Gibberellin 3-beta-dioxygenase 4 OS=Morus notabilis GN=L484_019467 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT1G52820.11.8e-7543.59 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily prot... [more]
AT1G52790.12.7e-7442.99 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily prot... [more]
AT1G52800.11.0e-7343.93 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily prot... [more]
AT4G03070.13.8e-6540.38 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily prot... [more]
AT1G80320.12.0e-6140.19 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily prot... [more]
Match NameE-valueIdentityDescription
gi|778664781|ref|XP_011660352.1|9.6e-13573.62PREDICTED: probable 2-oxoglutarate-dependent dioxygenase AOP1 [Cucumis sativus][more]
gi|778657573|ref|XP_004151475.2|5.9e-11674.91PREDICTED: probable 2-oxoglutarate-dependent dioxygenase AOP1.2 [Cucumis sativus... [more]
gi|147852210|emb|CAN80139.1|2.1e-8952.40hypothetical protein VITISV_028513 [Vitis vinifera][more]
gi|225442749|ref|XP_002284974.1|2.1e-8952.40PREDICTED: probable 2-oxoglutarate-dependent dioxygenase AOP1 [Vitis vinifera][more]
gi|567869013|ref|XP_006427128.1|1.5e-8753.04hypothetical protein CICLE_v10027501mg [Citrus clementina][more]
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR005123Oxoglu/Fe-dep_dioxygenase
IPR026992DIOX_N
IPR027443IPNS-like
Vocabulary: Molecular Function
TermDefinition
GO:0016491oxidoreductase activity
Vocabulary: Biological Process
TermDefinition
GO:0055114oxidation-reduction process
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0055114 oxidation-reduction process
cellular_component GO:0005575 cellular_component
molecular_function GO:0016706 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, 2-oxoglutarate as one donor, and incorporation of one atom each of oxygen into both donors
molecular_function GO:0046872 metal ion binding
molecular_function GO:0016491 oxidoreductase activity
molecular_function GO:0051213 dioxygenase activity

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmoCh13G009720.1CmoCh13G009720.1mRNA


Analysis Name: InterPro Annotations of Cucurbita moschata
Date Performed: 2017-05-19
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR005123Oxoglutarate/iron-dependent dioxygenasePFAMPF031712OG-FeII_Oxycoord: 167..263
score: 1.4
IPR005123Oxoglutarate/iron-dependent dioxygenasePROFILEPS51471FE2OG_OXYcoord: 159..266
score: 10
IPR026992Non-haem dioxygenase N-terminal domainPFAMPF14226DIOX_Ncoord: 5..84
score: 2.
IPR027443Isopenicillin N synthase-likeGENE3DG3DSA:2.60.120.330coord: 4..301
score: 3.2
NoneNo IPR availablePANTHERPTHR10209OXIDOREDUCTASE, 2OG-FE II OXYGENASE FAMILY PROTEINcoord: 4..305
score: 1.1E
NoneNo IPR availablePANTHERPTHR10209:SF182SUBFAMILY NOT NAMEDcoord: 4..305
score: 1.1E
NoneNo IPR availableunknownSSF51197Clavaminate synthase-likecoord: 4..300
score: 6.73