Carg07731 (gene) Silver-seed gourd | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Carg07731
Type	gene
Organism	Cucurbita argyrosperma (Silver-seed gourd)
Description	2-oxoglutarate-dependent dioxygenase
Location	Cucurbita_argyrosperma_scaffold_024 : 1125668 .. 1126814 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCGGGTGAGCTTCCTGTTGTGAGATTTAGTTCACACAATATGCAGAGCGGAGGGAGGAAGTGGGGTTCGACGAGGGAGAAGGTCCGGGAGGCACTTGAAGAATATGGGTGCTTTGTAGCGTTGTGCGACAGCGTTTCAATGGAGGCGAGTTCTTTGAAGATGATGGAAGGCATGAAGGAGGTGTTCGAAGTTCCATTGGAGAGGAAGGTTCAGAATGTGTCTGAGAAGCCTTTCCATGGCTACTTTGGGCAGAACCCATTGATGCCTATCCATGAAAGCATGGGCATTGAAGACCCTCTTCTTCCCTCCAACATCCAATCTTTCTCCAACCTCATGTGGCCCTCTCGCCCAAACCCTCTCTTCTGGTAAATTTCTTCCCTTCATTCATTCTGCCTTTATTTCTCACTTTCAGAGCTAACCCTCATGCTATTTTGCAGTGAAAACCTCAAATCCTACGCACAATTGGTGTCCCAATTGGAGCAGACGGTGAAGAAGATGGTGTTCGAGAGCTACGGCCTCAAAAGCCTCTTCCTTTCTCACACCCAATCCACCAAATATCTTCTCCGGATGATCAAATACAGAGTCCCACATCGGAACGAGATGAATTTGGGAGCTTTCCCTCACACCGACAAGAGCTTCCTCACCATTCTTCATCAGAATTCAGTAAATGGGTTACACATTAAGACCAGAGACAACAGATGGGTTGAATATCAGCCTCCTTCTACAGAATCTTCTGCCAAATCATTCATCGTCATGGCTGGAGATGCCTTCTTTGTAAGTTCCCATTAACTTCAAATTCCATTACAATTAGCAATAACCCATCAAAAACTTACCAACAATTGTGGGGTTGTTGGGACAGGCGTGGAGCAATGGGAGGATATATTCGGCGCCGCACAGAGTGACGATGAGTGGAAACAGAGAGAGATACTCAGTGGGGTTGTTTTCGTTCAACGATGGGATTGTTCAGATTCCAAAAGAATTGGTGGACGACAAACATCCGCAGCAGTTTGAGGCGTTTGATCATCAAGATTACCTTCGTTTCTACAGCACTGAGAAAGGCCAAAGCTCCCCTTCTGCTATTAAAGCTTATTGCGGCGTTGCAAATGGCCTCCTTGATTCACGAACTTGCATTCCCATGCATTAA

mRNA sequence

ATGGCGGGTGAGCTTCCTGTTGTGAGATTTAGTTCACACAATATGCAGAGCGGAGGGAGGAAGTGGGGTTCGACGAGGGAGAAGGTCCGGGAGGCACTTGAAGAATATGGGTGCTTTGTAGCGTTGTGCGACAGCGTTTCAATGGAGGCGAGTTCTTTGAAGATGATGGAAGGCATGAAGGAGGTGTTCGAAGTTCCATTGGAGAGGAAGGTTCAGAATGTGTCTGAGAAGCCTTTCCATGGCTACTTTGGGCAGAACCCATTGATGCCTATCCATGAAAGCATGGGCATTGAAGACCCTCTTCTTCCCTCCAACATCCAATCTTTCTCCAACCTCATGTGGCCCTCTCGCCCAAACCCTCTCTTCTGTGAAAACCTCAAATCCTACGCACAATTGGTGTCCCAATTGGAGCAGACGGTGAAGAAGATGGTGTTCGAGAGCTACGGCCTCAAAAGCCTCTTCCTTTCTCACACCCAATCCACCAAATATCTTCTCCGGATGATCAAATACAGAGTCCCACATCGGAACGAGATGAATTTGGGAGCTTTCCCTCACACCGACAAGAGCTTCCTCACCATTCTTCATCAGAATTCAGTAAATGGGTTACACATTAAGACCAGAGACAACAGATGGGTTGAATATCAGCCTCCTTCTACAGAATCTTCTGCCAAATCATTCATCGTCATGGCTGGAGATGCCTTCTTTGCGTGGAGCAATGGGAGGATATATTCGGCGCCGCACAGAGTGACGATGAGTGGAAACAGAGAGAGATACTCAGTGGGGTTGTTTTCGTTCAACGATGGGATTGTTCAGATTCCAAAAGAATTGGTGGACGACAAACATCCGCAGCAGTTTGAGGCGTTTGATCATCAAGATTACCTTCGTTTCTACAGCACTGAGAAAGGCCAAAGCTCCCCTTCTGCTATTAAAGCTTATTGCGGCGTTGCAAATGGCCTCCTTGATTCACGAACTTGCATTCCCATGCATTAA

Coding sequence (CDS)

ATGGCGGGTGAGCTTCCTGTTGTGAGATTTAGTTCACACAATATGCAGAGCGGAGGGAGGAAGTGGGGTTCGACGAGGGAGAAGGTCCGGGAGGCACTTGAAGAATATGGGTGCTTTGTAGCGTTGTGCGACAGCGTTTCAATGGAGGCGAGTTCTTTGAAGATGATGGAAGGCATGAAGGAGGTGTTCGAAGTTCCATTGGAGAGGAAGGTTCAGAATGTGTCTGAGAAGCCTTTCCATGGCTACTTTGGGCAGAACCCATTGATGCCTATCCATGAAAGCATGGGCATTGAAGACCCTCTTCTTCCCTCCAACATCCAATCTTTCTCCAACCTCATGTGGCCCTCTCGCCCAAACCCTCTCTTCTGTGAAAACCTCAAATCCTACGCACAATTGGTGTCCCAATTGGAGCAGACGGTGAAGAAGATGGTGTTCGAGAGCTACGGCCTCAAAAGCCTCTTCCTTTCTCACACCCAATCCACCAAATATCTTCTCCGGATGATCAAATACAGAGTCCCACATCGGAACGAGATGAATTTGGGAGCTTTCCCTCACACCGACAAGAGCTTCCTCACCATTCTTCATCAGAATTCAGTAAATGGGTTACACATTAAGACCAGAGACAACAGATGGGTTGAATATCAGCCTCCTTCTACAGAATCTTCTGCCAAATCATTCATCGTCATGGCTGGAGATGCCTTCTTTGCGTGGAGCAATGGGAGGATATATTCGGCGCCGCACAGAGTGACGATGAGTGGAAACAGAGAGAGATACTCAGTGGGGTTGTTTTCGTTCAACGATGGGATTGTTCAGATTCCAAAAGAATTGGTGGACGACAAACATCCGCAGCAGTTTGAGGCGTTTGATCATCAAGATTACCTTCGTTTCTACAGCACTGAGAAAGGCCAAAGCTCCCCTTCTGCTATTAAAGCTTATTGCGGCGTTGCAAATGGCCTCCTTGATTCACGAACTTGCATTCCCATGCATTAA

Protein sequence

MAGELPVVRFSSHNMQSGGRKWGSTREKVREALEEYGCFVALCDSVSMEASSLKMMEGMKEVFEVPLERKVQNVSEKPFHGYFGQNPLMPIHESMGIEDPLLPSNIQSFSNLMWPSRPNPLFCENLKSYAQLVSQLEQTVKKMVFESYGLKSLFLSHTQSTKYLLRMIKYRVPHRNEMNLGAFPHTDKSFLTILHQNSVNGLHIKTRDNRWVEYQPPSTESSAKSFIVMAGDAFFAWSNGRIYSAPHRVTMSGNRERYSVGLFSFNDGIVQIPKELVDDKHPQQFEAFDHQDYLRFYSTEKGQSSPSAIKAYCGVANGLLDSRTCIPMH

Homology

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BLAST of Carg07731 vs. NCBI nr
Match: XP_022923887.1 (probable 2-oxoglutarate-dependent dioxygenase AOP1 [Cucurbita moschata])

HSP 1 Score: 668.3 bits (1723), Expect = 1.3e-188
Identity = 322/329 (97.87%), Postives = 327/329 (99.39%), Query Frame = 0


Query: 1   MAGELPVVRFSSHNMQSGGRKWGSTREKVREALEEYGCFVALCDSVSMEASSLKMMEGMK 60
           MAGELPVVRFSSHN+QSGGRKWGSTREKVREALEEYGCFVALCDSVSMEASSLKMME M+
Sbjct: 1   MAGELPVVRFSSHNLQSGGRKWGSTREKVREALEEYGCFVALCDSVSMEASSLKMMEAME 60

Query: 61  EVFEVPLERKVQNVSEKPFHGYFGQNPLMPIHESMGIEDPLLPSNIQSFSNLMWPSRPNP 120
           EVFEVPLERKVQNVSEKPFHGYFGQNPLMPIHESMGIEDPLLPSNIQSFSNLMWPSRPNP
Sbjct: 61  EVFEVPLERKVQNVSEKPFHGYFGQNPLMPIHESMGIEDPLLPSNIQSFSNLMWPSRPNP 120

Query: 121 LFCENLKSYAQLVSQLEQTVKKMVFESYGLKSLFLSHTQSTKYLLRMIKYRVPHRNEMNL 180
           LFCENLKSYA+LVSQLEQTVKKMVFESYGLKSLFLSHTQSTKYLLRMIKYRVPHRNEMNL
Sbjct: 121 LFCENLKSYAELVSQLEQTVKKMVFESYGLKSLFLSHTQSTKYLLRMIKYRVPHRNEMNL 180

Query: 181 GAFPHTDKSFLTILHQNSVNGLHIKTRDNRWVEYQPPSTESSAKSFIVMAGDAFFAWSNG 240
           GAFPHTDKSFLTILHQNSVNGLHIKTRDNRWVEYQPPS ESSAKSFI+MAGDAFFAWSNG
Sbjct: 181 GAFPHTDKSFLTILHQNSVNGLHIKTRDNRWVEYQPPSAESSAKSFIIMAGDAFFAWSNG 240

Query: 241 RIYSAPHRVTMSGNRERYSVGLFSFNDGIVQIPKELVDDKHPQQFEAFDHQDYLRFYSTE 300
           RIYSAPHRVTMSGNRERYSVGLFSFNDGIVQIPKELVDDKHPQQF+AFDHQDYLRFYSTE
Sbjct: 241 RIYSAPHRVTMSGNRERYSVGLFSFNDGIVQIPKELVDDKHPQQFKAFDHQDYLRFYSTE 300

Query: 301 KGQSSPSAIKAYCGVANGLLDSRTCIPMH 330
           KGQSSPSAIKAYCGVANGLLDSRTCIPMH
Sbjct: 301 KGQSSPSAIKAYCGVANGLLDSRTCIPMH 329

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BLAST of Carg07731 vs. NCBI nr
Match: XP_022923888.1 (probable 2-oxoglutarate-dependent dioxygenase AOP1 [Cucurbita moschata] >XP_023519885.1 probable 2-oxoglutarate-dependent dioxygenase AOP1 [Cucurbita pepo subsp. pepo] >XP_023519886.1 probable 2-oxoglutarate-dependent dioxygenase AOP1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 665.2 bits (1715), Expect = 1.1e-187
Identity = 322/329 (97.87%), Postives = 326/329 (99.09%), Query Frame = 0


Query: 1   MAGELPVVRFSSHNMQSGGRKWGSTREKVREALEEYGCFVALCDSVSMEASSLKMMEGMK 60
           MAGE+PVVRFSSHN+QSGGRKWGSTREKVREALEEYGCFVAL DSVSMEASSLKMME MK
Sbjct: 1   MAGEIPVVRFSSHNLQSGGRKWGSTREKVREALEEYGCFVALYDSVSMEASSLKMMEAMK 60

Query: 61  EVFEVPLERKVQNVSEKPFHGYFGQNPLMPIHESMGIEDPLLPSNIQSFSNLMWPSRPNP 120
           EVFEVPLERKVQNVSEKPFHGYFGQNPLMPIHESMGIEDPLLPSNIQSFSNLMWPSRPNP
Sbjct: 61  EVFEVPLERKVQNVSEKPFHGYFGQNPLMPIHESMGIEDPLLPSNIQSFSNLMWPSRPNP 120

Query: 121 LFCENLKSYAQLVSQLEQTVKKMVFESYGLKSLFLSHTQSTKYLLRMIKYRVPHRNEMNL 180
           LFCENLKSYA+LVSQLEQTVKKMVFESYGLKSLFLSHTQSTKYLLRMIKYRVPHRNEMNL
Sbjct: 121 LFCENLKSYAELVSQLEQTVKKMVFESYGLKSLFLSHTQSTKYLLRMIKYRVPHRNEMNL 180

Query: 181 GAFPHTDKSFLTILHQNSVNGLHIKTRDNRWVEYQPPSTESSAKSFIVMAGDAFFAWSNG 240
           GAFPHTDKSFLTILHQNSVNGLHIKTRDNRWVEYQPPS ESSAKSFIVMAGDAFFAWSNG
Sbjct: 181 GAFPHTDKSFLTILHQNSVNGLHIKTRDNRWVEYQPPSAESSAKSFIVMAGDAFFAWSNG 240

Query: 241 RIYSAPHRVTMSGNRERYSVGLFSFNDGIVQIPKELVDDKHPQQFEAFDHQDYLRFYSTE 300
           RIYSAPHRVTMSGNRERYSVGLFSFNDGIVQIPKELVDDKHPQQF+AFDHQDYLRFYSTE
Sbjct: 241 RIYSAPHRVTMSGNRERYSVGLFSFNDGIVQIPKELVDDKHPQQFKAFDHQDYLRFYSTE 300

Query: 301 KGQSSPSAIKAYCGVANGLLDSRTCIPMH 330
           KGQSSPSAIKAYCGVANGLLDSRTCIPMH
Sbjct: 301 KGQSSPSAIKAYCGVANGLLDSRTCIPMH 329

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BLAST of Carg07731 vs. NCBI nr
Match: XP_023000968.1 (probable 2-oxoglutarate-dependent dioxygenase AOP1 [Cucurbita maxima] >XP_023001072.1 probable 2-oxoglutarate-dependent dioxygenase AOP1 [Cucurbita maxima])

HSP 1 Score: 661.8 bits (1706), Expect = 1.2e-186
Identity = 318/329 (96.66%), Postives = 326/329 (99.09%), Query Frame = 0


Query: 1   MAGELPVVRFSSHNMQSGGRKWGSTREKVREALEEYGCFVALCDSVSMEASSLKMMEGMK 60
           MAGE+PVVRFSSHN+QSGGRKWGSTREKVREALEEYGCFVALCDSVSMEASS+KMME MK
Sbjct: 1   MAGEIPVVRFSSHNVQSGGRKWGSTREKVREALEEYGCFVALCDSVSMEASSVKMMEAMK 60

Query: 61  EVFEVPLERKVQNVSEKPFHGYFGQNPLMPIHESMGIEDPLLPSNIQSFSNLMWPSRPNP 120
           EVFEVPLERKVQNVS+KPFHGYFGQNPLMPIHESMGIEDPLLPSNIQSFSNLMWPSRPNP
Sbjct: 61  EVFEVPLERKVQNVSQKPFHGYFGQNPLMPIHESMGIEDPLLPSNIQSFSNLMWPSRPNP 120

Query: 121 LFCENLKSYAQLVSQLEQTVKKMVFESYGLKSLFLSHTQSTKYLLRMIKYRVPHRNEMNL 180
           LFC NLKSYA+LVSQLEQTVKKMVFESYGLKSLFLSHTQSTKYLLRMIKYRVPHRNEMNL
Sbjct: 121 LFCGNLKSYAELVSQLEQTVKKMVFESYGLKSLFLSHTQSTKYLLRMIKYRVPHRNEMNL 180

Query: 181 GAFPHTDKSFLTILHQNSVNGLHIKTRDNRWVEYQPPSTESSAKSFIVMAGDAFFAWSNG 240
           GAFPHTDKSFLTILHQNSVNGLHIKTRDNRWVEYQPPS +SSA+SFIVMAGDAFFAWSNG
Sbjct: 181 GAFPHTDKSFLTILHQNSVNGLHIKTRDNRWVEYQPPSAQSSARSFIVMAGDAFFAWSNG 240

Query: 241 RIYSAPHRVTMSGNRERYSVGLFSFNDGIVQIPKELVDDKHPQQFEAFDHQDYLRFYSTE 300
           RIYSAPHRVTMSGNRERYSVGLFSFNDGIVQIPKELVDDKHPQQF+AFDHQDYLRFYSTE
Sbjct: 241 RIYSAPHRVTMSGNRERYSVGLFSFNDGIVQIPKELVDDKHPQQFKAFDHQDYLRFYSTE 300

Query: 301 KGQSSPSAIKAYCGVANGLLDSRTCIPMH 330
           KGQSSPSAIKAYCGVANGLLDSRTCIPMH
Sbjct: 301 KGQSSPSAIKAYCGVANGLLDSRTCIPMH 329

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BLAST of Carg07731 vs. NCBI nr
Match: XP_023522901.1 (probable 2-oxoglutarate-dependent dioxygenase AOP1.2, partial [Cucurbita pepo subsp. pepo])

HSP 1 Score: 520.4 bits (1339), Expect = 4.5e-144
Identity = 253/259 (97.68%), Postives = 256/259 (98.84%), Query Frame = 0


Query: 1   MAGELPVVRFSSHNMQSGGRKWGSTREKVREALEEYGCFVALCDSVSMEASSLKMMEGMK 60
           MAGE+PVVRFSSHN+QSGGRKWGSTREKVREALEEYGCFVAL DSVSMEASSLKMME MK
Sbjct: 1   MAGEIPVVRFSSHNLQSGGRKWGSTREKVREALEEYGCFVALYDSVSMEASSLKMMEAMK 60

Query: 61  EVFEVPLERKVQNVSEKPFHGYFGQNPLMPIHESMGIEDPLLPSNIQSFSNLMWPSRPNP 120
           EVFEVPLERKVQNVSEKPFHGYFGQNPLMPIHESMGIEDPLLPSNIQSFSNLMWPSRPNP
Sbjct: 61  EVFEVPLERKVQNVSEKPFHGYFGQNPLMPIHESMGIEDPLLPSNIQSFSNLMWPSRPNP 120

Query: 121 LFCENLKSYAQLVSQLEQTVKKMVFESYGLKSLFLSHTQSTKYLLRMIKYRVPHRNEMNL 180
           LFCENLKSYA+LVSQLEQTVKKMVFESYGLKSLFLSHTQSTKYLLRMIKYRVPHRNEMNL
Sbjct: 121 LFCENLKSYAELVSQLEQTVKKMVFESYGLKSLFLSHTQSTKYLLRMIKYRVPHRNEMNL 180

Query: 181 GAFPHTDKSFLTILHQNSVNGLHIKTRDNRWVEYQPPSTESSAKSFIVMAGDAFFAWSNG 240
           GAFPHTDKSFLTILHQNSVNGLHIKTRDNRWVEYQPPS ESSAKSFIVMAGDAFFAWSNG
Sbjct: 181 GAFPHTDKSFLTILHQNSVNGLHIKTRDNRWVEYQPPSAESSAKSFIVMAGDAFFAWSNG 240

Query: 241 RIYSAPHRVTMSGNRERYS 260
           RIYSAPHRVTMSGNRERYS
Sbjct: 241 RIYSAPHRVTMSGNRERYS 259

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BLAST of Carg07731 vs. NCBI nr
Match: XP_022137575.1 (probable 2-oxoglutarate-dependent dioxygenase AOP1 isoform X1 [Momordica charantia])

HSP 1 Score: 501.5 bits (1290), Expect = 2.2e-138
Identity = 239/326 (73.31%), Postives = 276/326 (84.66%), Query Frame = 0


Query: 4   ELPVVRFSSHNMQSGGRKWGSTREKVREALEEYGCFVALCDSVSMEASSLKMMEGMKEVF 63
           +LP+V+FS  N+    R W STREKVREALEEYGCFVAL DSVSME SS  M + +KE+F
Sbjct: 5   QLPLVKFSEENL----RSWVSTREKVREALEEYGCFVALYDSVSMELSS-NMFDSLKELF 64

Query: 64  EVPLERKVQNVSEKPFHGYFGQNPLMPIHESMGIEDPLLPSNIQSFSNLMWPSRPNPLFC 123
           EVPLERK++NVSEKP+HGYFGQNPLMPIHESMGIEDP L S  QSF+NLMWPS  N  FC
Sbjct: 65  EVPLERKLKNVSEKPYHGYFGQNPLMPIHESMGIEDPNLLSKTQSFTNLMWPSNGNKSFC 124

Query: 124 ENLKSYAQLVSQLEQTVKKMVFESYGLKSLFLSHTQSTKYLLRMIKYRVPHRNEMNLGAF 183
           EN+ SYA+ +++L++TVKKMVFESYG++    SH +ST+YLLRMIKYRVP +NEMN+GAF
Sbjct: 125 ENVNSYARAIAELDETVKKMVFESYGVRKHLESHMKSTRYLLRMIKYRVPKQNEMNMGAF 184

Query: 184 PHTDKSFLTILHQNSVNGLHIKTRDNRWVEYQPPSTESSAKSFIVMAGDAFFAWSNGRIY 243
           PHTDKSFLTILHQN VNGL IKT+ N+W++Y P S      SFIVMAGDAFFAWSNGRIY
Sbjct: 185 PHTDKSFLTILHQNQVNGLQIKTKHNKWLQYHPSSXXXXXXSFIVMAGDAFFAWSNGRIY 244

Query: 244 SAPHRVTMSGNRERYSVGLFSFNDGIVQIPKELVDDKHPQQFEAFDHQDYLRFYSTEKGQ 303
           S PH+V MSG RERYSVGLFSFNDGIVQIPKELVD+ HPQQF+ FDHQDYLRFYSTE+GQ
Sbjct: 245 SPPHKVIMSGKRERYSVGLFSFNDGIVQIPKELVDENHPQQFKPFDHQDYLRFYSTERGQ 304

Query: 304 SSPSAIKAYCGVANGLLDSRTCIPMH 330
           +SP AIKAYCG+ NGL+D+R CI +H
Sbjct: 305 NSPCAIKAYCGIGNGLVDTRGCILLH 325

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BLAST of Carg07731 vs. TAIR10
Match: AT1G52820.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein)

HSP 1 Score: 280.8 bits (717), Expect = 1.1e-75
Identity = 135/313 (43.13%), Postives = 208/313 (66.45%), Query Frame = 0


Query: 5   LPVVRFSSHNMQSGGRKWGSTREKVREALEEYGCFVALCDSVSMEASSLKMMEGMKEVFE 64
           LPV+ FS+ N++ G  +W  TR  V++AL++YG F A  D +  E     +   ++E+F+
Sbjct: 12  LPVIDFSNKNLKPGEPEWDLTRADVQKALQDYGYFEASFDRIPFELRK-SVFGALEELFD 71

Query: 65  VPLERKVQNVSEKPFHGYFGQNPLMPIHESMGIEDPLLPSNIQSFSNLMWPSRPNPLFCE 124
           +PL+ K++NVS+KPFHGY GQ P++P++ESMGI+D  +   + +F+  +WP + N  F  
Sbjct: 72  LPLQTKLRNVSKKPFHGYVGQYPMVPLYESMGIDDSDIAEKVDAFTEKLWP-QGNISFST 131

Query: 125 NLKSYAQLVSQLEQTVKKMVFESYGLKSLFLSHTQSTKYLLRMIKYRVPHRNEMNLGAFP 184
            ++S+++ +S+L+ T+++M+ ES+GL      H  ST YLLR++KY+ P   E  +G   
Sbjct: 132 TIQSFSKKLSELDITIRRMIMESFGLDKYIDEHLHSTNYLLRVMKYKGPDTEETKVGLNA 191

Query: 185 HTDKSFLTILHQNSVNGLHIKTRDNRWVEYQPPSTESSAKSFIVMAGDAFFAWSNGRIYS 244
           HTDK+ +TIL+QN V GL ++T+D  W++ +P     +  SF VM GD+ +A  NGR++S
Sbjct: 192 HTDKNIVTILYQNHVEGLEVQTKDKNWIKVKP-----TQDSFTVMIGDSLYALLNGRLHS 251

Query: 245 APHRVTMSGNRERYSVGLFSFNDG--IVQIPKELVDDKHPQQFEAFDHQDYLRFYSTEKG 304
             HRV M+G   RYS+GLFS      IV  P ELVD++HP+ F+ FDH ++L+FY TE G
Sbjct: 252 PYHRVMMTGTETRYSLGLFSIPKAGHIVSSPDELVDEEHPRLFKPFDHVEFLQFYYTEAG 311

Query: 305 QSSPSAIKAYCGV 316
           Q S SA+K YCG+
Sbjct: 312 QRSQSALKTYCGI 317

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BLAST of Carg07731 vs. TAIR10
Match: AT1G52790.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein)

HSP 1 Score: 278.1 bits (710), Expect = 7.0e-75
Identity = 135/315 (42.86%), Postives = 206/315 (65.40%), Query Frame = 0


Query: 1   MAGELPVVRFSSHNMQSGGRKWGSTREKVREALEEYGCFVA-LCDSVSMEASSLKMMEGM 60
           M+ ++P + FS  +++ G + W STRE +R+ALEEYGCF+  L D   ++    ++   +
Sbjct: 1   MSPKIPTLDFSREDLKPGTKYWESTRENIRQALEEYGCFIIDLKDKTPLDLLD-RVFGSL 60

Query: 61  KEVFEVPLERKVQNVSEKPFHGYFGQNPLMPIHESMGIEDPLLPSNIQSFSNLMWPSRPN 120
            ++F++P + K++N  +KP +GY GQ P +P+HES+GI++       +SF+ LMWP + N
Sbjct: 61  VDLFDLPTQTKMKNKYDKPLNGYVGQIPALPLHESLGIDNATSLEATRSFTGLMWP-QGN 120

Query: 121 PLFCENLKSYAQLVSQLEQTVKKMVFESYGLKSLFLSHTQSTKYLLRMIKYRVPHRNEMN 180
             F E L  YA+  ++L+Q V +MVF+SY ++  +  + +ST YLLR++K R P+     
Sbjct: 121 EHFSECLYKYAEFAAELDQMVTRMVFQSYNVEKYYDPYIESTTYLLRVLKNRAPNNENPT 180

Query: 181 LGAFPHTDKSFLTILHQNSVNGLHIKTRDNRWVEYQPPSTESSAKSFIVMAGDAFFAWSN 240
           LG   HTDKSF TILHQ+ VNGL ++TR+   +        SS   F+V+AGDA  AWSN
Sbjct: 181 LGFVTHTDKSFTTILHQDQVNGLEMETREGERINI----NLSSPSLFMVVAGDALMAWSN 240

Query: 241 GRIYSAPHRVTMSGNRERYSVGLFSFNDGIVQIPKELVDDKHPQQFEAFDHQDYLRFYST 300
            R++S  H+V +SG  +RYS+G+F+FN+G +Q+P+EL+D +HP  ++ FDH   LRFY T
Sbjct: 241 DRVWSPRHQVLVSGETDRYSLGMFAFNNGTLQVPEELIDHQHPLMYKPFDHLGLLRFYRT 300

Query: 301 EKGQSSPSAIKAYCG 315
           + G  S   IKAYCG
Sbjct: 301 DTGYKSECPIKAYCG 309

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BLAST of Carg07731 vs. TAIR10
Match: AT1G52800.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein)

HSP 1 Score: 277.7 bits (709), Expect = 9.2e-75
Identity = 133/306 (43.46%), Postives = 202/306 (66.01%), Query Frame = 0


Query: 11  SSHNMQSGGRKWGSTREKVREALEEYGCFVALCDSVSMEASSLKMMEGMKEVFEVPLERK 70
           S   ++     W S   +  EALEEYGCF+A+ D V+ +     +    +E+F++P E K
Sbjct: 17  SQQELKPNTSTWRSVSREACEALEEYGCFLAVYDGVTQQLDD-SIFAAAEELFDLPTETK 76

Query: 71  VQNVSEKPFHGYFGQNPLMPIHESMGIEDPLLPSNIQSFSNLMWPSRPNPLFCENLKSYA 130
            +NV+EKP+HGY GQ P++P+HE +G++        Q F++LMWP + N  FC  + +++
Sbjct: 77  KKNVNEKPYHGYVGQMPVIPLHEGLGVDYVTNKEIAQRFTHLMWP-QGNDRFCNTVHTFS 136

Query: 131 QLVSQLEQTVKKMVFESYGLKSLFLSHTQSTKYLLRMIKYRVPHRNEMNLGAFP-HTDKS 190
             V++L++ V +M+FE+YG++  + SH  S  YLL+ +KY  P  + +++ AFP HTDK+
Sbjct: 137 NAVAELDRLVVRMIFENYGVEKHYESHVGSKTYLLKFLKYLAPPES-ISMPAFPQHTDKT 196

Query: 191 FLTILHQNSVNGLHIKTRDNRWVEYQPPSTESSAKSFIVMAGDAFFAWSNGRIYSAPHRV 250
           FL+ILHQN VNGL +K++D  W+  Q P      KS++VMAGD    WSN RI S  HRV
Sbjct: 197 FLSILHQNDVNGLEVKSKDGEWISLQLP-----PKSYVVMAGDISMGWSNDRIRSCEHRV 256

Query: 251 TMSGNRERYSVGLFSFNDGIVQIPKELVDDKHPQQFEAFDHQDYLRFYSTEKGQSSPSAI 310
           TM G++ RY++GLFSF   +V IP+ELVDDKHP  ++ FD+   + FY+T++G+ + S +
Sbjct: 257 TMEGDKTRYTLGLFSFLTDLVSIPEELVDDKHPLMYKPFDNIALINFYTTKEGREANSTL 314

Query: 311 KAYCGV 316
           KAYCG+
Sbjct: 317 KAYCGI 314

[Close]

BLAST of Carg07731 vs. TAIR10
Match: AT1G80320.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein)

HSP 1 Score: 245.4 bits (625), Expect = 5.0e-65
Identity = 131/317 (41.32%), Postives = 190/317 (59.94%), Query Frame = 0


Query: 5   LPVVRFSSHNMQSGGRKWGSTREKVREALEEYGCFVALCDSVSMEASSLKMMEGMKEVFE 64
           LP++ FSS  +  G   W +TR+ VR A+E  G FVA    VS +     ++ GMKE++ 
Sbjct: 9   LPILDFSSDKLVRGTSHWITTRDDVRRAMEGQGWFVAEFSGVSSDLRD-NLLAGMKEMYY 68

Query: 65  VPLERKVQNVSEKPFHGYFGQ-NPLMPIHESMGIEDPLLPSNIQSFSNLMWPSRPNPLFC 124
           +P + K++N + K  HGY         IHES+GI+        + FS L+WP   +P FC
Sbjct: 69  LPDQIKIKNENHKASHGYMSMVVDDYRIHESLGIDYATELQACKDFSKLLWPQGNDP-FC 128

Query: 125 ENLKSYAQLVSQLEQTVKKMVFESYGL--KSLFLSHTQSTKYLLRMIKYRVPHRNEMNLG 184
           +    YA  +++L+QTV +M++ESYG+  K   +SH++ST+YLLRM+ YR     E N G
Sbjct: 129 QTTHMYAMTMAELDQTVMRMLYESYGMDEKKHSVSHSESTRYLLRMLSYRRQQNGEANTG 188

Query: 185 AFPHTDKSFLTILHQNSVNGLHIKTRDNRWVEYQPPSTESSAKSFIVMAGDAFFAWSNGR 244
              HTDKSF++ILHQN V GL +KT   +WV + P  T      F+V++G    AWSN R
Sbjct: 189 FVSHTDKSFMSILHQNHVGGLQLKTMTGQWVGFNPSPTR-----FVVLSGMGLTAWSNDR 248

Query: 245 IYSAPHRVTMSGNRERYSVGLFSFNDGIVQIPKELVDDKHPQQFEAFDHQDYLRFYST-- 304
           I +  H+V MS +  RYS+G FSF+ G ++ P+ELVDD+HP ++  F+H   LRFY +  
Sbjct: 249 IKACYHKVVMSADEIRYSLGFFSFHKGTIRTPEELVDDQHPLRYNPFEHDGLLRFYESYI 308

Query: 305 -EKGQSSPSAIKAYCGV 316
               +SS   ++ YCGV
Sbjct: 309 NSLKKSSEDLLQIYCGV 318

[Close]

BLAST of Carg07731 vs. TAIR10
Match: AT4G03070.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein)

HSP 1 Score: 240.0 bits (611), Expect = 2.1e-63
Identity = 122/317 (38.49%), Postives = 190/317 (59.94%), Query Frame = 0


Query: 4   ELPVVRFSSHNMQSGGRKWGSTREKVREALEEYGCFVALCDSVSMEASSLKMMEGMKEVF 63
           +LPV+ FS  N++ G  KW      V +ALE+YGCF A  D +S+E +   + E M+++F
Sbjct: 13  QLPVIDFSDQNLKPGSSKWDEVTADVLKALEDYGCFEASFDKLSVELNR-SVFEAMEDLF 72

Query: 64  EVPLERKVQNVSEKPFHGYFGQNPLMPIHESMGIEDPLLPSNIQSFSNLMWPSRPNPLFC 123
           E+P+  K +NVS KPFHGY   N    ++ES+GI D  +   +  F+  +WP   N    
Sbjct: 73  ELPIPTKQRNVSSKPFHGYLCHN----LYESLGINDANVLEKVNDFTQQLWPDHGNKSIS 132

Query: 124 ENLKSYAQLVSQLEQTVKKMVFESYGLKSLFLSHTQSTKYLLRMIKY------RVPHRNE 183
           E +  +++ + +L+  V++M+ ES+G+++    H  ST YL R++KY             
Sbjct: 133 ETIHLFSEQLVELDLMVRRMIMESFGIENYIDEHLNSTYYLTRLMKYXXXXXXXXXXXXX 192

Query: 184 MNLGAFPHTDKSFLTILHQNSVNGLHIKTRDNRWVEYQPPSTESSAKSFIVMAGDAFFAW 243
             LG   HTDK+ +TILHQ  V+GL +KT+D++W++ +P     S  S +VM GD+  A 
Sbjct: 193 XKLGLRSHTDKNIITILHQYQVDGLEVKTKDDKWIKVKP-----SQDSVLVMVGDSLCAL 252

Query: 244 SNGRIYSAPHRVTMSGNRERYSVGLFSF--NDGIVQIPKELVDDKHPQQFEAFDHQDYLR 303
            NGR++S  HRV M+G + RYS GLFS      I+  P+ELVD +HP+ F+ F++ D+L 
Sbjct: 253 LNGRLHSPYHRVIMTGKKTRYSTGLFSIPKTGVIIDSPEELVDKEHPRIFKPFEYTDFLH 312

Query: 304 FYSTEKGQSSPSAIKAY 313
           F+ TE G+ + SA+ A+
Sbjct: 313 FFQTEAGRIAQSALHAF 319

[Close]

BLAST of Carg07731 vs. Swiss-Prot
Match: sp|Q945B6|AOP1L_ARATH (Probable 2-oxoglutarate-dependent dioxygenase AOP1.2 OS=Arabidopsis thaliana OX=3702 GN=AOP1.2 PE=2 SV=1)

HSP 1 Score: 243.0 bits (619), Expect = 4.5e-63
Identity = 122/316 (38.61%), Postives = 192/316 (60.76%), Query Frame = 0


Query: 4   ELPVVRFSSHNMQSGGRKWGSTREKVREALEEYGCFVALCDSVSMEASSLKMMEGMKEVF 63
           +LPV+ FS  N++ G  KW   +  V +ALE+YGCF A  D +S+E +   + E M+++F
Sbjct: 13  QLPVIDFSDQNLKPGSSKWDEVKADVLKALEDYGCFEAFFDKLSVELNR-SVFEAMEDLF 72

Query: 64  EVPLERKVQNVSEKPFHGYFGQNPLMPIHESMGIEDPLLPSNIQSFSNLMWPSRPNPLFC 123
           E+P+  K +NVS KPFHGY   N    ++ES+GI+D  +   +  F+  +WP   N    
Sbjct: 73  ELPIPTKQRNVSSKPFHGYLCHN----LYESLGIDDANVLEKVNDFTQQLWPDHGNKSIS 132

Query: 124 ENLKSYAQLVSQLEQTVKKMVFESYGLKSLFLSHTQSTKYLLRMIKY-----RVPHRNEM 183
           E +  +++ + +L+  V++M+ ES+G+++    H  ST YL R++KY             
Sbjct: 133 ETIHLFSEQLVELDLMVRRMIMESFGIENYIDEHLNSTYYLTRLMKYXXXXXXXXXXXXX 192

Query: 184 NLGAFPHTDKSFLTILHQNSVNGLHIKTRDNRWVEYQPPSTESSAKSFIVMAGDAFFAWS 243
            LG   HTDK+ +TILHQ  V+GL +KT+D++W++ +P     S  S +VM GD+  A  
Sbjct: 193 KLGLRSHTDKNIITILHQYQVDGLEVKTKDDKWIKVKP-----SQDSVLVMVGDSLCALL 252

Query: 244 NGRIYSAPHRVTMSGNRERYSVGLFSF--NDGIVQIPKELVDDKHPQQFEAFDHQDYLRF 303
           NGR++S  HRV M+G + RYS GLFS      I+  P+ELVD +HP+ F+ F++ D+L F
Sbjct: 253 NGRLHSPYHRVIMTGKKTRYSTGLFSIPKTGVIIDSPEELVDKEHPRIFKPFEYTDFLHF 312

Query: 304 YSTEKGQSSPSAIKAY 313
           + TE G+ + SA+ A+
Sbjct: 313 FQTEAGRIAQSALHAF 318

[Close]

BLAST of Carg07731 vs. Swiss-Prot
Match: sp|Q9ZTA3|AOP1C_ARATH (Probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Arabidopsis thaliana OX=3702 GN=AOP1 PE=2 SV=1)

HSP 1 Score: 240.0 bits (611), Expect = 3.8e-62
Identity = 122/317 (38.49%), Postives = 190/317 (59.94%), Query Frame = 0


Query: 4   ELPVVRFSSHNMQSGGRKWGSTREKVREALEEYGCFVALCDSVSMEASSLKMMEGMKEVF 63
           +LPV+ FS  N++ G  KW      V +ALE+YGCF A  D +S+E +   + E M+++F
Sbjct: 13  QLPVIDFSDQNLKPGSSKWDEVTADVLKALEDYGCFEASFDKLSVELNR-SVFEAMEDLF 72

Query: 64  EVPLERKVQNVSEKPFHGYFGQNPLMPIHESMGIEDPLLPSNIQSFSNLMWPSRPNPLFC 123
           E+P+  K +NVS KPFHGY   N    ++ES+GI D  +   +  F+  +WP   N    
Sbjct: 73  ELPIPTKQRNVSSKPFHGYLCHN----LYESLGINDANVLEKVNDFTQQLWPDHGNKSIS 132

Query: 124 ENLKSYAQLVSQLEQTVKKMVFESYGLKSLFLSHTQSTKYLLRMIKY------RVPHRNE 183
           E +  +++ + +L+  V++M+ ES+G+++    H  ST YL R++KY             
Sbjct: 133 ETIHLFSEQLVELDLMVRRMIMESFGIENYIDEHLNSTYYLTRLMKYXXXXXXXXXXXXX 192

Query: 184 MNLGAFPHTDKSFLTILHQNSVNGLHIKTRDNRWVEYQPPSTESSAKSFIVMAGDAFFAW 243
             LG   HTDK+ +TILHQ  V+GL +KT+D++W++ +P     S  S +VM GD+  A 
Sbjct: 193 XKLGLRSHTDKNIITILHQYQVDGLEVKTKDDKWIKVKP-----SQDSVLVMVGDSLCAL 252

Query: 244 SNGRIYSAPHRVTMSGNRERYSVGLFSF--NDGIVQIPKELVDDKHPQQFEAFDHQDYLR 303
            NGR++S  HRV M+G + RYS GLFS      I+  P+ELVD +HP+ F+ F++ D+L 
Sbjct: 253 LNGRLHSPYHRVIMTGKKTRYSTGLFSIPKTGVIIDSPEELVDKEHPRIFKPFEYTDFLH 312

Query: 304 FYSTEKGQSSPSAIKAY 313
           F+ TE G+ + SA+ A+
Sbjct: 313 FFQTEAGRIAQSALHAF 319

[Close]

BLAST of Carg07731 vs. Swiss-Prot
Match: sp|Q944Z5|AOP1V_ARATH (Probable 2-oxoglutarate-dependent dioxygenase AOP1 (Fragment) OS=Arabidopsis thaliana OX=3702 GN=AOP1 PE=2 SV=1)

HSP 1 Score: 230.7 bits (587), Expect = 2.3e-59
Identity = 118/306 (38.56%), Postives = 182/306 (59.48%), Query Frame = 0


Query: 4   ELPVVRFSSHNMQSGGRKWGSTREKVREALEEYGCFVALCDSVSMEASSLKMMEGMKEVF 63
           +LPV+ FS  N++ G  KW      V +ALE+YGCF A  D +S+E +   + E M+++F
Sbjct: 6   QLPVIDFSDQNLKPGSSKWDEVTADVLKALEDYGCFEASFDKLSVELNR-SVFEAMEDLF 65

Query: 64  EVPLERKVQNVSEKPFHGYFGQNPLMPIHESMGIEDPLLPSNIQSFSNLMWPSRPNPLFC 123
           E+P+  K +NVS K FHGY   N    ++ES+GI D  +   +  F+  +WP   N    
Sbjct: 66  ELPIPTKQRNVSSKLFHGYLCHN----LYESLGINDANVLEKVNDFTQQLWPDHGNKSIS 125

Query: 124 ENLKSYAQLVSQLEQTVKKMVFESYGLKSLFLSHTQSTKYLLRMIKY----RVPHRNEMN 183
           E +  +++ + +L+  V++M+ ES+G++     H  ST YL R++KY             
Sbjct: 126 ETIHLFSEQLVELDLMVRRMIMESFGIEKYIDEHLNSTYYLTRLMKYTXXXXXXXXXXTK 185

Query: 184 LGAFPHTDKSFLTILHQNSVNGLHIKTRDNRWVEYQPPSTESSAKSFIVMAGDAFFAWSN 243
           LG   HTDK+ +TILHQ  V+GL +KT+D++W++ +P     S  S +VM GD+  A  N
Sbjct: 186 LGLRSHTDKNIITILHQYQVDGLEVKTKDDKWIKVKP-----SQDSVLVMVGDSLCALLN 245

Query: 244 GRIYSAPHRVTMSGNRERYSVGLFSF--NDGIVQIPKELVDDKHPQQFEAFDHQDYLRFY 303
           GR++S  HRV M+G + RYS GLFS      I+  P+ELVD +HP+ F+ F++ D+L F+
Sbjct: 246 GRLHSPYHRVIMTGKKTRYSTGLFSIPKTGVIIDSPEELVDKEHPRIFKPFEYTDFLHFF 301

[Close]

BLAST of Carg07731 vs. Swiss-Prot
Match: sp|Q945B4|AOP3L_ARATH (2-oxoglutarate-dependent dioxygenase AOP3 OS=Arabidopsis thaliana OX=3702 GN=AOP3 PE=2 SV=1)

HSP 1 Score: 194.9 bits (494), Expect = 1.4e-48
Identity = 124/414 (29.95%), Postives = 193/414 (46.62%), Query Frame = 0


Query: 4   ELPVVRFSSHNMQSGGRKWGSTREKVREALEEYGCFVALCDSVSMEASSLKMMEGMKEVF 63
           +LP++  S   ++ G  KW   R  VR+ALE+YGCF A  D VSME     +++ M+E+F
Sbjct: 7   QLPLICLSDQTLKPGSSKWVKVRSDVRKALEDYGCFEAKIDQVSMELQG-SVLKAMQELF 66

Query: 64  EVPLERKVQNVSEKPFHGYFGQNPLMPIHESMGIEDPLLPSNIQSFSNLMWPSRPNPLFC 123
            +P E K +NV  KPF GYF  N L    ES GI+D  +      F+  +WP     +  
Sbjct: 67  ALPTEAKQRNVCPKPFAGYFSHNGL---SESFGIKDANILEKAHEFTQQLWPEGNKSI-- 126

Query: 124 ENLKSYAQLVSQLEQTVKKMVFESYGLKSLFLSHTQSTKYLLRMIKYRVPHRNEM----- 183
           + ++ YA+ +++L+  V++++ ESYG++     H  ST Y +R++KY     N++     
Sbjct: 127 KMIQLYAEKLAELDMMVRRLILESYGIEYFIDEHLNSTYYRMRLMKYIARPDNDITAAVG 186

Query: 184 ------------------------------------------------------------ 243
                                                                       
Sbjct: 187 ANVDNGXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 246

Query: 244 -------------------------------NLGAFPHTDKSFLTILHQNSVNGLHIKTR 303
                                           LG   HTDKS   I++Q+ ++GL +KT+
Sbjct: 247 XXXXXXXXXXXXXXXXXXXXXVGAKEANVDAELGLPSHTDKSLSGIIYQHQIDGLEVKTK 306

Query: 304 DNRWVEYQPPSTESSAKSFIVMAGDAFFAWSNGRIYSAPHRVTMSGNRE-RYSVGLFSF- 319
           + +W+  +P     +  + IV+AGDA  A  NGRI S  HRV ++  ++ RY+  LFS+ 
Sbjct: 307 EGKWIRVKP-----APNTVIVIAGDALCALMNGRIPSPYHRVRVTERKKTRYAAALFSYP 366

[Close]

BLAST of Carg07731 vs. Swiss-Prot
Match: sp|Q9ZTA1|AOP3C_ARATH (2-oxoglutarate-dependent dioxygenase AOP3 OS=Arabidopsis thaliana OX=3702 GN=AOP3 PE=2 SV=1)

HSP 1 Score: 192.6 bits (488), Expect = 7.0e-48
Identity = 124/414 (29.95%), Postives = 191/414 (46.14%), Query Frame = 0


Query: 4   ELPVVRFSSHNMQSGGRKWGSTREKVREALEEYGCFVALCDSVSMEASSLKMMEGMKEVF 63
           +LP++  S   ++ G  KW   R  VR+ALE+YGCF A  D VSME     +++ M+E+F
Sbjct: 7   QLPLICLSDQTLKPGSSKWVKVRSDVRKALEDYGCFEAKIDQVSMELQG-SVLKAMQELF 66

Query: 64  EVPLERKVQNVSEKPFHGYFGQNPLMPIHESMGIEDPLLPSNIQSFSNLMWPSRPNPLFC 123
            +P E K +NV  KPF GY   N L    ES GI+D  +      F+  +WP   N    
Sbjct: 67  ALPTEAKQRNVCPKPFTGYLSHNGL---SESFGIKDANILEKAHEFTQQLWP-EGNKSIS 126

Query: 124 ENLKSYAQLVSQLEQTVKKMVFESYGLKSLFLSHTQSTKYLLRMIKYRVPHRNEM----- 183
           + ++ YA+ +++L+  V++++ ESYG++     H  ST Y +R++KY     N++     
Sbjct: 127 KMIQLYAEKLAELDMMVRRLILESYGIEYFIDEHLNSTYYRMRLMKYIARPDNDITAAVG 186

Query: 184 ------------------------------------------------------------ 243
                                                                       
Sbjct: 187 ANVDNGXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 246

Query: 244 -------------------------------NLGAFPHTDKSFLTILHQNSVNGLHIKTR 303
                                           LG   HTDKS   I++Q+ ++GL +KT+
Sbjct: 247 XXXXXXXXXXXXXXXXXXXXXVGAKEANVDAELGLPSHTDKSLTGIIYQHQIDGLEVKTK 306

Query: 304 DNRWVEYQPPSTESSAKSFIVMAGDAFFAWSNGRIYSAPHRVTMSGNRE-RYSVGLFSF- 319
           + +W+  +P     +  + IV+AGDA  A  NGRI S  HRV ++  ++ RY+  LFS  
Sbjct: 307 EGKWIRVKP-----APNTVIVIAGDALCALMNGRIPSPYHRVRVTEKKKTRYAAALFSNP 366

[Close]

BLAST of Carg07731 vs. TrEMBL
Match: tr|A0A2I4FFJ5|A0A2I4FFJ5_9ROSI (probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Juglans regia OX=51240 GN=LOC108998345 PE=3 SV=1)

HSP 1 Score: 365.2 bits (936), Expect = 1.6e-97
Identity = 181/316 (57.28%), Postives = 237/316 (75.00%), Query Frame = 0


Query: 2   AGELPVVRFSSHNMQSGGRKWGSTREKVREALEEYGCFVALCDSVSMEASSLKMMEGMKE 61
           A +LPV+ FS  N+QS    W STR+KVR+ALEEYGCFVAL + VS +  +  + + + E
Sbjct: 7   AAKLPVIDFSGDNLQS---YWASTRDKVRQALEEYGCFVALYEKVSSDLDN-DVFDSLVE 66

Query: 62  VFEVPLERKVQNVSEKPFHGYFGQNPLMPIHESMGIEDPLLPSNIQSFSNLMWPSRPNPL 121
           +F++PLE K++NVSEKP+HGYFGQNP +P++ESMGIED  + S  QSF+NLMWPS  N  
Sbjct: 67  LFDLPLETKLKNVSEKPYHGYFGQNPWLPLNESMGIEDAQVLSRTQSFTNLMWPS-GNQN 126

Query: 122 FCENLKSYAQLVSQLEQTVKKMVFESYGLKSLFLSHTQSTKYLLRMIKYRVPHRNEM-NL 181
           FC+++ SY++ VS+L+  V+ MVFESYG+K  F SH +ST  LLR+IKY  P + ++ N+
Sbjct: 127 FCKSIHSYSKKVSELDYLVESMVFESYGVKKYFDSHIKSTTNLLRLIKYNEPKKGDVNNI 186

Query: 182 GAFPHTDKSFLTILHQNSVNGLHIKTRDNRWVEYQP-PSTESSAKSFIVMAGDAFFAWSN 241
           GA  HTDKSF+TILHQN VNGL I+T+D RW++++P PS      SF+VMAG+A  AWSN
Sbjct: 187 GAISHTDKSFITILHQNEVNGLEIETKDGRWIDFEPFPS------SFVVMAGEASTAWSN 246

Query: 242 GRIYSAPHRVTMSGNRERYSVGLFSFNDGIVQIPKELVDDKHPQQFEAFDHQDYLRFYST 301
           GRIYS  HRV M   ++RYS+G+FSFN G +Q PKELVDD+HP QF+ FDH   LRFYST
Sbjct: 247 GRIYSPFHRVIMKEGKDRYSLGMFSFNSGTIQTPKELVDDEHPLQFKPFDHLSLLRFYST 306

Query: 302 EKGQSSPSAIKAYCGV 316
           ++GQ     IKAYCG+
Sbjct: 307 DEGQKHHCTIKAYCGL 311

[Close]

BLAST of Carg07731 vs. TrEMBL
Match: tr|D7U0Q7|D7U0Q7_VITVI (Uncharacterized protein OS=Vitis vinifera OX=29760 GN=VIT_09s0002g05290 PE=3 SV=1)

HSP 1 Score: 342.4 bits (877), Expect = 1.1e-90
Identity = 165/314 (52.55%), Postives = 222/314 (70.70%), Query Frame = 0


Query: 2   AGELPVVRFSSHNMQSGGRKWGSTREKVREALEEYGCFVALCDSVSMEASSLKMMEGMKE 61
           A  LPV+ FS+  ++ G R W    + V  ALEEYGCFVA+ D VS E  +    + ++E
Sbjct: 5   APRLPVLNFSTGTLKPGTRSWLLACKDVLHALEEYGCFVAVYDKVSSELDNTTFCQ-LEE 64

Query: 62  VFEVPLERKVQNVSEKPFHGYFGQNPLMPIHESMGIEDPLLPSNIQSFSNLMWPSRPNPL 121
           +F++P E KV+N S+KP+ GY GQ+PL+P+HESMGIED      ++SF+N+MWP+  N  
Sbjct: 65  LFDLPTETKVKNTSDKPYFGYIGQHPLIPLHESMGIEDATTLKGVESFTNIMWPA-GNDH 124

Query: 122 FCENLKSYAQLVSQLEQTVKKMVFESYGLKSLFLSHTQSTKYLLRMIKYRVPHRNEMNLG 181
           FCE + SY++LVS+LEQ VKKMVFESY  +  + SH QST YLLR+IKYR P +NE  +G
Sbjct: 125 FCETVLSYSKLVSELEQMVKKMVFESYDAEKYYDSHIQSTTYLLRLIKYRGPQKNETCIG 184

Query: 182 AFPHTDKSFLTILHQNSVNGLHIKTRDNRWVEYQPPSTESSAKSFIVMAGDAFFAWSNGR 241
              HTDKSF+T+LHQN V GL I+T+D  W+ ++P     S  S++VMAGDA  AWSNGR
Sbjct: 185 TSCHTDKSFITVLHQNEVCGLEIRTKDGEWISFKP-----SPSSYVVMAGDALLAWSNGR 244

Query: 242 IYSAPHRVTMSGNRERYSVGLFSFNDGIVQIPKELVDDKHPQQFEAFDHQDYLRFYSTEK 301
           I+S  HRV M+GN+ RYS+GLF+++ GI++IP+EL D++HP QF+ F+H   L FY T K
Sbjct: 245 IHSPEHRVIMNGNKARYSIGLFAYHRGIIEIPEELADEEHPLQFKPFNHYGLLHFYFTNK 304

Query: 302 GQSSPSAIKAYCGV 316
              + S  KAYCGV
Sbjct: 305 AGQAESTAKAYCGV 311

[Close]

BLAST of Carg07731 vs. TrEMBL
Match: tr|A5AJI0|A5AJI0_VITVI (Uncharacterized protein OS=Vitis vinifera OX=29760 GN=VITISV_028513 PE=3 SV=1)

HSP 1 Score: 342.4 bits (877), Expect = 1.1e-90
Identity = 165/314 (52.55%), Postives = 222/314 (70.70%), Query Frame = 0


Query: 2   AGELPVVRFSSHNMQSGGRKWGSTREKVREALEEYGCFVALCDSVSMEASSLKMMEGMKE 61
           A  LPV+ FS+  ++ G R W    + V  ALEEYGCFVA+ D VS E  +    + ++E
Sbjct: 5   APRLPVLNFSTGTLKPGTRSWLLACKDVLHALEEYGCFVAVYDKVSSELDNTTFCQ-LEE 64

Query: 62  VFEVPLERKVQNVSEKPFHGYFGQNPLMPIHESMGIEDPLLPSNIQSFSNLMWPSRPNPL 121
           +F++P E KV+N S+KP+ GY GQ+PL+P+HESMGIED      ++SF+N+MWP+  N  
Sbjct: 65  LFDLPTETKVKNTSDKPYFGYIGQHPLIPLHESMGIEDATTLKGVESFTNIMWPA-GNDH 124

Query: 122 FCENLKSYAQLVSQLEQTVKKMVFESYGLKSLFLSHTQSTKYLLRMIKYRVPHRNEMNLG 181
           FCE + SY++LVS+LEQ VKKMVFESY  +  + SH QST YLLR+IKYR P +NE  +G
Sbjct: 125 FCETVLSYSKLVSELEQMVKKMVFESYDAEKYYDSHIQSTTYLLRLIKYRGPQKNETCIG 184

Query: 182 AFPHTDKSFLTILHQNSVNGLHIKTRDNRWVEYQPPSTESSAKSFIVMAGDAFFAWSNGR 241
              HTDKSF+T+LHQN V GL I+T+D  W+ ++P     S  S++VMAGDA  AWSNGR
Sbjct: 185 TSCHTDKSFITVLHQNEVCGLEIRTKDGEWISFKP-----SPSSYVVMAGDALLAWSNGR 244

Query: 242 IYSAPHRVTMSGNRERYSVGLFSFNDGIVQIPKELVDDKHPQQFEAFDHQDYLRFYSTEK 301
           I+S  HRV M+GN+ RYS+GLF+++ GI++IP+EL D++HP QF+ F+H   L FY T K
Sbjct: 245 IHSPEHRVIMNGNKARYSIGLFAYHRGIIEIPEELADEEHPLQFKPFNHYGLLHFYFTNK 304

Query: 302 GQSSPSAIKAYCGV 316
              + S  KAYCGV
Sbjct: 305 AGQTESTAKAYCGV 311

[Close]

BLAST of Carg07731 vs. TrEMBL
Match: tr|A0A0A0LT96|A0A0A0LT96_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G044830 PE=4 SV=1)

HSP 1 Score: 335.1 bits (858), Expect = 1.8e-88
Identity = 167/236 (70.76%), Postives = 195/236 (82.63%), Query Frame = 0


Query: 4   ELPVVRFSSHNMQSGGRKWGSTREKVREALEEYGCFVALCDSVSMEASSLKMMEGMKEVF 63
           +LPVV+F S N++SG  +W ST +KVREALEEYGCFVAL D+VS E SS  M + +KE+F
Sbjct: 9   QLPVVKFCSQNLKSGTEEWVSTTKKVREALEEYGCFVALYDTVSAEVSS-NMFDSLKELF 68

Query: 64  -EVPLERKVQNVSEKPFHGYFGQNPLMPIHESMGIEDPLLPSNIQSFSNLMWPSRPNPLF 123
            EVPLERK+QNVSEKP+HGYFGQNPLMPIHESMGIE P+LP+NI SF+NLMWPSR N  F
Sbjct: 69  DEVPLERKLQNVSEKPYHGYFGQNPLMPIHESMGIEHPILPTNINSFTNLMWPSRGNHSF 128

Query: 124 CENLKSYAQLVSQLEQTVKKMVFESYGLKSLFLSHTQSTKYLLRMIKYRVPHRNEMNLGA 183
            EN+ +YA LVS+L++ VK MVFESYG+ +   SH +STKYL+RMIKYRVP   EMNLGA
Sbjct: 129 RENVTAYANLVSELDKRVKSMVFESYGVGNALRSHMESTKYLMRMIKYRVPKEKEMNLGA 188

Query: 184 FPHTDKSFLTILHQNSVNGLHIKTRDNRWVEYQPPSTESSAKSFIVMAGDAFFAWS 239
           FPHTDKSFLTILHQN VNGL IKTRDN+W++Y P S+ SS  SFIVMAGDAFF  S
Sbjct: 189 FPHTDKSFLTILHQNEVNGLQIKTRDNKWIQYHPFSS-SSTSSFIVMAGDAFFVSS 242

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BLAST of Carg07731 vs. TrEMBL
Match: tr|V4RZ66|V4RZ66_9ROSI (Uncharacterized protein OS=Citrus clementina OX=85681 GN=CICLE_v10027501mg PE=3 SV=1)

HSP 1 Score: 334.3 bits (856), Expect = 3.0e-88
Identity = 166/314 (52.87%), Postives = 223/314 (71.02%), Query Frame = 0


Query: 4   ELPVVRFSSHNMQSGGRKWGSTREKVREALEEYGCFVALCDSVSMEASSLKMMEGMKEVF 63
           +LPVV  S  N + G   W  T  KVREALEE+GCFVAL D VS+E  +  +    +E+F
Sbjct: 31  KLPVVNLSKENFKPGASSWLGTSRKVREALEEFGCFVALYDEVSLELHN-AIFSAAEELF 90

Query: 64  EVPLERKVQNVSEKPFHGYFGQNPLMP-IHESMGIEDPLLPSNIQSFSNLMWPSRPNPLF 123
            +P+E K +NVSEKP++GY G NPL+P I+E MG++        Q+F+NL+WP   N  F
Sbjct: 91  NLPIEIKEKNVSEKPYYGYLGNNPLVPAIYEGMGVDYANTIEGTQNFTNLIWP-EGNENF 150

Query: 124 CENLKSYAQLVSQLEQTVKKMVFESYGLKSLFLSHTQSTKYLLRMIKYRVPHRNEMNLGA 183
           C+ + SY++LVS+LEQ VK+MVFESYG+   + S  +S+ YLLR++KYR P  NE NLG 
Sbjct: 151 CKTVVSYSRLVSELEQMVKRMVFESYGVDKYYDSVLESSTYLLRIMKYRCPETNEKNLGC 210

Query: 184 FPHTDKSFLTILHQNSVNGLHIKTRDNRWVEYQPPSTESSAKSFIVMAGDAFFAWSNGRI 243
             HTDKSF+T+LHQN VNGL I+T+D  W+ +  P    +  SFIV+AGDAF AWSN RI
Sbjct: 211 DVHTDKSFITVLHQNEVNGLEIRTKDGCWIGFDDP----TPSSFIVLAGDAFLAWSNSRI 270

Query: 244 YSAPHRVTMSGNRE-RYSVGLFSFNDGIVQIPKELVDDKHPQQFEAFDHQDYLRFYSTEK 303
           +S  HRV ++G ++ RYS+GLF+FNDGI+QIP+ELVDD+HP +F++F+H   LR+Y TE+
Sbjct: 271 HSPVHRVILNGIKQARYSLGLFTFNDGIIQIPEELVDDEHPLKFKSFEHFGLLRYYLTEE 330

Query: 304 GQSSPSAIKAYCGV 316
           GQ +    KAYC V
Sbjct: 331 GQKAECTAKAYCSV 338

The following BLAST results are available for this feature:

BLAST of Carg07731 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022923887.1	1.3e-188	97.87	probable 2-oxoglutarate-dependent dioxygenase AOP1 [Cucurbita moschata]	[more]
XP_022923888.1	1.1e-187	97.87	probable 2-oxoglutarate-dependent dioxygenase AOP1 [Cucurbita moschata] >XP_0235...	[more]
XP_023000968.1	1.2e-186	96.66	probable 2-oxoglutarate-dependent dioxygenase AOP1 [Cucurbita maxima] >XP_023001...	[more]
XP_023522901.1	4.5e-144	97.68	probable 2-oxoglutarate-dependent dioxygenase AOP1.2, partial [Cucurbita pepo su...	[more]
XP_022137575.1	2.2e-138	73.31	probable 2-oxoglutarate-dependent dioxygenase AOP1 isoform X1 [Momordica charant...	[more]

BLAST of Carg07731 vs. TAIR10


Match Name	E-value	Identity	Description
AT1G52820.1	1.1e-75	43.13	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT1G52790.1	7.0e-75	42.86	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT1G52800.1	9.2e-75	43.46	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT1G80320.1	5.0e-65	41.32	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT4G03070.1	2.1e-63	38.49	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]

BLAST of Carg07731 vs. Swiss-Prot


Match Name	E-value	Identity	Description
sp\|Q945B6\|AOP1L_ARATH	4.5e-63	38.61	Probable 2-oxoglutarate-dependent dioxygenase AOP1.2 OS=Arabidopsis thaliana OX=...	[more]
sp\|Q9ZTA3\|AOP1C_ARATH	3.8e-62	38.49	Probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Arabidopsis thaliana OX=37...	[more]
sp\|Q944Z5\|AOP1V_ARATH	2.3e-59	38.56	Probable 2-oxoglutarate-dependent dioxygenase AOP1 (Fragment) OS=Arabidopsis tha...	[more]
sp\|Q945B4\|AOP3L_ARATH	1.4e-48	29.95	2-oxoglutarate-dependent dioxygenase AOP3 OS=Arabidopsis thaliana OX=3702 GN=AOP...	[more]
sp\|Q9ZTA1\|AOP3C_ARATH	7.0e-48	29.95	2-oxoglutarate-dependent dioxygenase AOP3 OS=Arabidopsis thaliana OX=3702 GN=AOP...	[more]

BLAST of Carg07731 vs. TrEMBL


Match Name	E-value	Identity	Description
tr\|A0A2I4FFJ5\|A0A2I4FFJ5_9ROSI	1.6e-97	57.28	probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Juglans regia OX=51240 GN=...	[more]
tr\|D7U0Q7\|D7U0Q7_VITVI	1.1e-90	52.55	Uncharacterized protein OS=Vitis vinifera OX=29760 GN=VIT_09s0002g05290 PE=3 SV=...	[more]
tr\|A5AJI0\|A5AJI0_VITVI	1.1e-90	52.55	Uncharacterized protein OS=Vitis vinifera OX=29760 GN=VITISV_028513 PE=3 SV=1	[more]
tr\|A0A0A0LT96\|A0A0A0LT96_CUCSA	1.8e-88	70.76	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G044830 PE=4 SV=1	[more]
tr\|V4RZ66\|V4RZ66_9ROSI	3.0e-88	52.87	Uncharacterized protein OS=Citrus clementina OX=85681 GN=CICLE_v10027501mg PE=3 ...	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **Biological Process**
Term	Definition
GO:0055114	oxidation-reduction process

Vocabulary: **Molecular Function**
Term	Definition
GO:0016491	oxidoreductase activity

Vocabulary: **INTERPRO**
Term	Definition
IPR005123	Oxoglu/Fe-dep_dioxygenase
IPR027443	IPNS-like

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0055114	oxidation-reduction process
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0016491	oxidoreductase activity

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg07731-RA	Carg07731-RA	mRNA

InterPro

Analysis Name: InterPro Annotations of silver-seed gourd
Date Performed: 2019-03-07


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR027443	Isopenicillin N synthase-like	GENE3D	G3DSA:2.60.120.330		coord: 1..296 e-value: 1.2E-48 score: 168.3
IPR005123	Oxoglutarate/iron-dependent dioxygenase	PFAM	PF03171	2OG-FeII_Oxy	coord: 169..265 e-value: 4.3E-18 score: 65.6
IPR005123	Oxoglutarate/iron-dependent dioxygenase	PROSITE	PS51471	FE2OG_OXY	coord: 161..268 score: 10.735
None	No IPR available	PANTHER	PTHR10209:SF182	2-OXOGLUTARATE 2OG AND FE II -DEPENDENT OXYGENASE SUPERFAMILY PROTEIN-RELATED	coord: 4..314
None	No IPR available	PANTHER	PTHR10209	OXIDOREDUCTASE, 2OG-FE II OXYGENASE FAMILY PROTEIN	coord: 4..314
None	No IPR available	SUPERFAMILY	SSF51197	Clavaminate synthase-like	coord: 4..302

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Carg07731	Cla008361	Watermelon (97103) v1	carwmB1006
Carg07731	Csa1G044830	Cucumber (Chinese Long) v2	carcuB1019
Carg07731	ClCG01G014610	Watermelon (Charleston Gray)	carwcgB0977
Carg07731	CSPI01G07760	Wild cucumber (PI 183967)	carcpiB1043
Carg07731	Cucsa.097750	Cucumber (Gy14) v1	carcgyB1120
Carg07731	CmaCh13G009410	Cucurbita maxima (Rimu)	carcmaB1393
Carg07731	CmoCh13G009720	Cucurbita moschata (Rifu)	carcmoB1349
Carg07731	Lsi02G002920	Bottle gourd (USVL1VR-Ls)	carlsiB919
Carg07731	Cp4.1LG20g02050	Cucurbita pepo (Zucchini)	carcpeB1401
Carg07731	CsGy1G007590	Cucumber (Gy14) v2	carcgybB0991
Carg07731	MELO3C035752.2	Melon (DHL92) v3.6.1	carmedB0986
Carg07731	CsaV3_1G007710	Cucumber (Chinese Long) v3	carcucB1048
Carg07731	Cla97C01G013620	Watermelon (97103) v2	carwmbB1057
Carg07731	Bhi08G001144	Wax gourd	carwgoB1181

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Carg07731	Silver-seed gourd	carcarB482
Carg07731	Cucurbita maxima (Rimu)	carcmaB1396
Carg07731	Cucurbita moschata (Rifu)	carcmoB1351
Carg07731	Cucurbita pepo (Zucchini)	carcpeB1394
Carg07731	Melon (DHL92) v3.5.1	carmeB0976