Cp4.1LG17g04430 (gene) Cucurbita pepo (Zucchini) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Cp4.1LG17g04430
Type	gene
Organism	Cucurbita pepo (Cucurbita pepo (Zucchini))
Description	Cytochrome b5
Location	Cp4.1LG17 : 3020331 .. 3021171 (-)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGATTCAAATTCTAAAGTCTATACTTTCGATGAAGTTGCAAAGCACAACCACGAGAAAGATTGCTGGCTTATCATCTCTGGTTATGTATGTTCCTTTTTTGTTTTAATTTTTTAAAAAATTCATTAATTTCGATTAAAATAAACCGATTGTTTGATAACTGTTTTTTAAGTTTACGGGTATTTCTATAACATTATCAAGATTTTGAGATTTTGATGAAATGCAACATGTTACTGTAACAAATCAGCTCGTTAGTAGCAATAAAGACTTTAAATTAAATATTTAGTTTAAATACTATTAAATTTCATTCTACGTTTCTTTTTTATATCTTGTTTTCTATTTTCTATTTATTATTTTTTTGAGATATATTTCTAATCTCTAGATGTTGATTTAGGTGTATGATGTAACTTCTTATTTACCGGATCACCCAGGTGGCGGTGAGCTTTTGGTATTGGCTGTGGGTAAGATGCTGTTGCATTCGTTTACTATCTTAACCAAAATATATCTTTTTAATAAATAGTTTTTTTCGTGGAAAAAATATCGGACAGTAATACAAAGATTTTGAGCATGGAACTTATATAACATATTTGAATTTGCAGAAAAAGATGCGACATTTGATTTTAAGAGTGTCGGACATAGTGAAACAGCTCAAGAGAGGATGAAAAAGTATCAGATAGGTAAAATCGACCCGTTGACTCTCCCGACGGAACAGAAACACGTGGAAACTACTCCACACAAACCAACTAAGAGTCTACTTTCCTTTTCGCCTTTACATGTTCTAATTCCCCTATTGCTCTTCGTGTTGGCTATTGCTTTTCCCTATTTGAAAATTAAGGCCTAA

mRNA sequence

ATGGATTCAAATTCTAAAGTCTATACTTTCGATGAAGTTGCAAAGCACAACCACGAGAAAGATTGCTGGCTTATCATCTCTGGTTATGTGTATGATGTAACTTCTTATTTACCGGATCACCCAGGTGGCGGTGAGCTTTTGGTATTGGCTGTGGAAAAAGATGCGACATTTGATTTTAAGAGTGTCGGACATAGTGAAACAGCTCAAGAGAGGATGAAAAAGTATCAGATAGGTAAAATCGACCCGTTGACTCTCCCGACGGAACAGAAACACGTGGAAACTACTCCACACAAACCAACTAAGAGTCTACTTTCCTTTTCGCCTTTACATGTTCTAATTCCCCTATTGCTCTTCGTGTTGGCTATTGCTTTTCCCTATTTGAAAATTAAGGCCTAA

Coding sequence (CDS)

ATGGATTCAAATTCTAAAGTCTATACTTTCGATGAAGTTGCAAAGCACAACCACGAGAAAGATTGCTGGCTTATCATCTCTGGTTATGTGTATGATGTAACTTCTTATTTACCGGATCACCCAGGTGGCGGTGAGCTTTTGGTATTGGCTGTGGAAAAAGATGCGACATTTGATTTTAAGAGTGTCGGACATAGTGAAACAGCTCAAGAGAGGATGAAAAAGTATCAGATAGGTAAAATCGACCCGTTGACTCTCCCGACGGAACAGAAACACGTGGAAACTACTCCACACAAACCAACTAAGAGTCTACTTTCCTTTTCGCCTTTACATGTTCTAATTCCCCTATTGCTCTTCGTGTTGGCTATTGCTTTTCCCTATTTGAAAATTAAGGCCTAA

Protein sequence

MDSNSKVYTFDEVAKHNHEKDCWLIISGYVYDVTSYLPDHPGGGELLVLAVEKDATFDFKSVGHSETAQERMKKYQIGKIDPLTLPTEQKHVETTPHKPTKSLLSFSPLHVLIPLLLFVLAIAFPYLKIKA

Homology

[Close]

BLAST of Cp4.1LG17g04430 vs. Swiss-Prot
Match: CYB5E_ARATH (Cytochrome b5 isoform E OS=Arabidopsis thaliana GN=CYTB5-E PE=1 SV=2)

HSP 1 Score: 115.2 bits (287), Expect = 5.6e-25
Identity = 60/127 (47.24%), Postives = 84/127 (66.14%), Query Frame = 1


Query: 1   MDSNSKVYTFDEVAKHNHEKDCWLIISGYVYDVTSYLPDHPGGGELLVLAVEKDATFDFK 60
           M S+ KV +F+EV+KHN  KDCWLIISG VYDVT ++ DHPGG E+L+ +  KDAT DF+
Sbjct: 1   MSSDRKVLSFEEVSKHNKTKDCWLIISGKVYDVTPFMDDHPGGDEVLLSSTGKDATNDFE 60

Query: 61  SVGHSETAQERMKKYQIGKIDPLTLPTEQKHVETTPHKPTKSL-----LSFSPLHVLIPL 120
            VGHS+TA++ M KY IG+ID  ++P  + +V   P +P  +           L  L+P+
Sbjct: 61  DVGHSDTARDMMDKYFIGEIDSSSVPATRTYV--APQQPAYNQDKTPEFIIKILQFLVPI 120

Query: 121 LLFVLAI 123
           L+  LA+
Sbjct: 121 LILGLAL 125

[Close]

BLAST of Cp4.1LG17g04430 vs. Swiss-Prot
Match: CYB5S_TOBAC (Cytochrome b5, seed isoform OS=Nicotiana tabacum PE=2 SV=1)

HSP 1 Score: 114.4 bits (285), Expect = 9.5e-25
Identity = 61/129 (47.29%), Postives = 85/129 (65.89%), Query Frame = 1


Query: 1   MDSNSKVYTFDEVAKHNHEKDCWLIISGYVYDVTSYLPDHPGGGELLVLAVEKDATFDFK 60
           M   SKV+T  EV+ HN+ KDCWLIISG VY+VT +L DHPGGGE+L+ A  KDAT DF+
Sbjct: 1   MGGQSKVFTLAEVSNHNNAKDCWLIISGKVYNVTKFLEDHPGGGEVLLSATGKDATDDFE 60

Query: 61  SVGHSETAQERMKKYQIGKIDPLTLPTEQKHV-ETTPH----KPTKSLLSFSPLHVLIPL 120
            +GHS +A+  + +Y +G ID  T+PT+ K+     PH    K T+ ++    L  L+PL
Sbjct: 61  DIGHSSSARAMLDEYYVGDIDSSTIPTKVKYTPPKQPHYNQDKTTEFIVKL--LQFLVPL 120

Query: 121 LLFVLAIAF 125
           +  +L +AF
Sbjct: 121 I--ILGVAF 125

[Close]

BLAST of Cp4.1LG17g04430 vs. Swiss-Prot
Match: CYB5_TOBAC (Cytochrome b5 OS=Nicotiana tabacum PE=2 SV=1)

HSP 1 Score: 114.4 bits (285), Expect = 9.5e-25
Identity = 60/129 (46.51%), Postives = 84/129 (65.12%), Query Frame = 1


Query: 1   MDSNSKVYTFDEVAKHNHEKDCWLIISGYVYDVTSYLPDHPGGGELLVLAVEKDATFDFK 60
           M   +KV+T  EV++HN+ KDCWL+ISG VYDVT +L DHPGG E+L+ A  KDAT DF+
Sbjct: 1   MGGETKVFTLAEVSQHNNAKDCWLVISGKVYDVTKFLDDHPGGDEVLLSATGKDATDDFE 60

Query: 61  SVGHSETAQERMKKYQIGKIDPLTLPTEQKHVETTPHKP-----TKSLLSFSPLHVLIPL 120
            VGHS +A+  + +Y +G ID  T+PT+ K+  T P++P       S      L  L+PL
Sbjct: 61  DVGHSSSARAMLDEYYVGDIDSATIPTKTKY--TPPNQPHYNQDKTSEFVVKLLQFLVPL 120

Query: 121 LLFVLAIAF 125
           +  +L +AF
Sbjct: 121 I--ILGVAF 125

[Close]

BLAST of Cp4.1LG17g04430 vs. Swiss-Prot
Match: CYB5_BRAOB (Cytochrome b5 OS=Brassica oleracea var. botrytis GN=CYB5 PE=1 SV=1)

HSP 1 Score: 114.0 bits (284), Expect = 1.2e-24
Identity = 60/127 (47.24%), Postives = 83/127 (65.35%), Query Frame = 1


Query: 1   MDSNSKVYTFDEVAKHNHEKDCWLIISGYVYDVTSYLPDHPGGGELLVLAVEKDATFDFK 60
           M S  KV  F+EV++HN  KDCWLIISG VYDVT ++ DHPGG E+L+ +  KDAT DF+
Sbjct: 1   MASEKKVLGFEEVSQHNKTKDCWLIISGKVYDVTPFMDDHPGGDEVLLSSTGKDATNDFE 60

Query: 61  SVGHSETAQERMKKYQIGKIDPLTLPTEQKHVETTPHKPTKSL-----LSFSPLHVLIPL 120
            VGHS+TA++ M+KY IG+ID  T+P  + +V   P +P  +           L  L+P+
Sbjct: 61  DVGHSDTARDMMEKYYIGEIDSSTVPATRTYV--APVQPAYNQDKTPEFMIKILQFLVPI 120

Query: 121 LLFVLAI 123
           L+  LA+
Sbjct: 121 LILGLAL 125

[Close]

BLAST of Cp4.1LG17g04430 vs. Swiss-Prot
Match: CYB5B_ARATH (Cytochrome b5 isoform B OS=Arabidopsis thaliana GN=CYTB5-B PE=1 SV=1)

HSP 1 Score: 112.5 bits (280), Expect = 3.6e-24
Identity = 55/127 (43.31%), Postives = 83/127 (65.35%), Query Frame = 1


Query: 1   MDSNSKVYTFDEVAKHNHEKDCWLIISGYVYDVTSYLPDHPGGGELLVLAVEKDATFDFK 60
           M   +K++T  EV++HN   DCW++I+G VY+VT +L DHPGG ++L+ +  KDAT DF+
Sbjct: 1   MGDEAKIFTLSEVSEHNQAHDCWIVINGKVYNVTKFLEDHPGGDDVLLSSTGKDATDDFE 60

Query: 61  SVGHSETAQERMKKYQIGKIDPLTLPTEQKHVETTPHKP-----TKSLLSFSPLHVLIPL 120
            VGHSE+A+E M++Y +G+IDP T+P + K+  T P +P       S      L  L+PL
Sbjct: 61  DVGHSESAREMMEQYYVGEIDPTTIPKKVKY--TPPKQPHYNQDKTSEFIIKLLQFLVPL 120

Query: 121 LLFVLAI 123
            +  LA+
Sbjct: 121 AILGLAV 125

[Close]

BLAST of Cp4.1LG17g04430 vs. TrEMBL
Match: A0A0A0KA20_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_6G107940 PE=3 SV=1)

HSP 1 Score: 210.3 bits (534), Expect = 1.4e-51
Identity = 100/131 (76.34%), Postives = 115/131 (87.79%), Query Frame = 1


Query: 1   MDSNSKVYTFDEVAKHNHEKDCWLIISGYVYDVTSYLPDHPGGGELLVLAVEKDATFDFK 60
           MDSNSKVY+FDEVAKHN +KDCWLIISGYVYDVTS+LPDHPGG ELL+LAVEKDATFDFK
Sbjct: 1   MDSNSKVYSFDEVAKHNQQKDCWLIISGYVYDVTSFLPDHPGGDELLLLAVEKDATFDFK 60

Query: 61  SVGHSETAQERMKKYQIGKIDPLTLPTEQKHVETTPHKPTKSLLSFSPLHVLIPLLLFVL 120
           SVGHSE A E+MK YQIGKID  TLP +QK+VE+ P++ T SL SFSP+H+L+P+LL  L
Sbjct: 61  SVGHSELAHEKMKMYQIGKIDMSTLPEKQKYVESAPYQRTPSLFSFSPIHLLLPILLLAL 120

Query: 121 AIAFPYLKIKA 132
           A AFPY K+KA
Sbjct: 121 AFAFPYFKLKA 131

[Close]

BLAST of Cp4.1LG17g04430 vs. TrEMBL
Match: M5X125_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa013234mg PE=3 SV=1)

HSP 1 Score: 140.2 bits (352), Expect = 1.8e-30
Identity = 70/129 (54.26%), Postives = 91/129 (70.54%), Query Frame = 1


Query: 1   MDSNSKVYTFDEVAKHNHEKDCWLIISGYVYDVTSYLPDHPGGGELLVLAVEKDATFDFK 60
           M S+S+VY FDEVAKHNH+KDCW+IISG VYDVT +L DHPGG E+L+LAVEKDAT DF 
Sbjct: 1   MASDSQVYHFDEVAKHNHKKDCWIIISGKVYDVTPFLEDHPGGDEVLLLAVEKDATDDFN 60

Query: 61  SVGHSETAQERMKKYQIGKIDPLTLPTEQKH---VETTPHKPTKSLLSFSPLHVLIPLLL 120
            VGHS++A+E+M+K+ +GK+D  T+P +  +    +TTP     S      L  L+PLL+
Sbjct: 61  DVGHSDSAKEQMEKFYVGKVDTSTIPEQPSYKLPAQTTPPSGQSSGFVVKLLQFLLPLLI 120

Query: 121 FVLAIAFPY 127
              A A  Y
Sbjct: 121 LGAAFALQY 129

[Close]

BLAST of Cp4.1LG17g04430 vs. TrEMBL
Match: W9RDL9_9ROSA (Cytochrome b5, seed isoform OS=Morus notabilis GN=L484_023457 PE=3 SV=1)

HSP 1 Score: 135.2 bits (339), Expect = 5.8e-29
Identity = 69/130 (53.08%), Postives = 92/130 (70.77%), Query Frame = 1


Query: 1   MDSNSKVYTFDEVAKHNHEKDCWLIISGYVYDVTSYLPDHPGGGELLVLAVEKDATFDFK 60
           M  +SKV+ FDEVAKHNH+KDCW+IISG VY+VT +L +HPGG E+L+LA EKDAT DF+
Sbjct: 1   MARDSKVFAFDEVAKHNHKKDCWIIISGKVYNVTEFLEEHPGGDEVLLLAAEKDATDDFE 60

Query: 61  SVGHSETAQERMKKYQIGKIDPLTLPTEQKHVETTPHKPTKSLL----SFSPLHVLIPLL 120
            VGHS+TA+E M+KY +GK+D  TLP E+   +  P  P++S      S   L  L+PLL
Sbjct: 61  DVGHSDTAREMMEKYYVGKVDVSTLP-EKPSYKPPPQPPSQSNQSSGGSVKILQFLLPLL 120

Query: 121 LFVLAIAFPY 127
           +  +A A  +
Sbjct: 121 ILAIAFALHF 129

[Close]

BLAST of Cp4.1LG17g04430 vs. TrEMBL
Match: A0A151TY23_CAJCA (Cytochrome b5 isoform 1 OS=Cajanus cajan GN=KK1_011257 PE=3 SV=1)

HSP 1 Score: 134.0 bits (336), Expect = 1.3e-28
Identity = 67/131 (51.15%), Postives = 86/131 (65.65%), Query Frame = 1


Query: 1   MDSNSKVYTFDEVAKHNHEKDCWLIISGYVYDVTSYLPDHPGGGELLVLAVEKDATFDFK 60
           M  NSK  TF+EVAKHNH+KDCW+I+ G VYD+T +L DHPGG E+LV A EKDAT DF+
Sbjct: 1   MALNSKTLTFEEVAKHNHKKDCWIIVHGKVYDITPFLDDHPGGDEVLVAATEKDATIDFE 60

Query: 61  SVGHSETAQERMKKYQIGKIDPLTLPTEQKHVETTPHKP-----TKSLLSFSPLHVLIPL 120
            VGHS++A E M++Y IGK+D  TLP +  H++  P +        S      L  L+PL
Sbjct: 61  DVGHSDSATEMMERYLIGKVDTATLPAKVNHIQQPPTQAHGVGNQSSGFVVKMLQFLLPL 120

Query: 121 LLFVLAIAFPY 127
           LL  LA A  +
Sbjct: 121 LLLGLAFALQH 131

[Close]

BLAST of Cp4.1LG17g04430 vs. TrEMBL
Match: A0A151SWJ2_CAJCA (Cytochrome b5 OS=Cajanus cajan GN=KK1_014582 PE=3 SV=1)

HSP 1 Score: 131.3 bits (329), Expect = 8.3e-28
Identity = 66/134 (49.25%), Postives = 87/134 (64.93%), Query Frame = 1


Query: 1   MDSNSKVYTFDEVAKHNHEKDCWLIISGYVYDVTSYLPDHPGGGELLVLAVEKDATFDFK 60
           M S +K +TF+EVAKHNH KDCW+I++G VYDVT +L DHPGG E+LV A EKDAT DF+
Sbjct: 1   MGSKTKTFTFEEVAKHNHRKDCWIIVNGKVYDVTPFLEDHPGGDEVLVTATEKDATSDFE 60

Query: 61  SVGHSETAQERMKKYQIGKIDPLTLPTEQKHVETTPHKPTKSLLS--------FSPLHVL 120
            +GHSE+A E M+ Y +G++D  TLPT+      TP  PT++  +           L  L
Sbjct: 61  DIGHSESAIEMMQTYLVGEVDTNTLPTQ---ATNTPRPPTQAPANSNQSAGFVLKMLQYL 120

Query: 121 IPLLLFVLAIAFPY 127
           +PLL+   A A  Y
Sbjct: 121 VPLLILGFAFALQY 131

[Close]

BLAST of Cp4.1LG17g04430 vs. TAIR10
Match: AT5G53560.1 (AT5G53560.1 cytochrome B5 isoform E)

HSP 1 Score: 115.2 bits (287), Expect = 3.1e-26
Identity = 60/127 (47.24%), Postives = 84/127 (66.14%), Query Frame = 1


Query: 1   MDSNSKVYTFDEVAKHNHEKDCWLIISGYVYDVTSYLPDHPGGGELLVLAVEKDATFDFK 60
           M S+ KV +F+EV+KHN  KDCWLIISG VYDVT ++ DHPGG E+L+ +  KDAT DF+
Sbjct: 1   MSSDRKVLSFEEVSKHNKTKDCWLIISGKVYDVTPFMDDHPGGDEVLLSSTGKDATNDFE 60

Query: 61  SVGHSETAQERMKKYQIGKIDPLTLPTEQKHVETTPHKPTKSL-----LSFSPLHVLIPL 120
            VGHS+TA++ M KY IG+ID  ++P  + +V   P +P  +           L  L+P+
Sbjct: 61  DVGHSDTARDMMDKYFIGEIDSSSVPATRTYV--APQQPAYNQDKTPEFIIKILQFLVPI 120

Query: 121 LLFVLAI 123
           L+  LA+
Sbjct: 121 LILGLAL 125

[Close]

BLAST of Cp4.1LG17g04430 vs. TAIR10
Match: AT2G32720.1 (AT2G32720.1 cytochrome B5 isoform B)

HSP 1 Score: 112.5 bits (280), Expect = 2.0e-25
Identity = 55/127 (43.31%), Postives = 83/127 (65.35%), Query Frame = 1


Query: 1   MDSNSKVYTFDEVAKHNHEKDCWLIISGYVYDVTSYLPDHPGGGELLVLAVEKDATFDFK 60
           M   +K++T  EV++HN   DCW++I+G VY+VT +L DHPGG ++L+ +  KDAT DF+
Sbjct: 1   MGDEAKIFTLSEVSEHNQAHDCWIVINGKVYNVTKFLEDHPGGDDVLLSSTGKDATDDFE 60

Query: 61  SVGHSETAQERMKKYQIGKIDPLTLPTEQKHVETTPHKP-----TKSLLSFSPLHVLIPL 120
            VGHSE+A+E M++Y +G+IDP T+P + K+  T P +P       S      L  L+PL
Sbjct: 61  DVGHSESAREMMEQYYVGEIDPTTIPKKVKY--TPPKQPHYNQDKTSEFIIKLLQFLVPL 120

Query: 121 LLFVLAI 123
            +  LA+
Sbjct: 121 AILGLAV 125

[Close]

BLAST of Cp4.1LG17g04430 vs. TAIR10
Match: AT5G48810.1 (AT5G48810.1 cytochrome B5 isoform D)

HSP 1 Score: 109.8 bits (273), Expect = 1.3e-24
Identity = 56/131 (42.75%), Postives = 77/131 (58.78%), Query Frame = 1


Query: 1   MDSNSKVYTFDEVAKHNHEKDCWLIISGYVYDVTSYLPDHPGGGELLVLAVEKDATFDFK 60
           M  + KV+T  EV++H+  KDCW++I G VYDVT +L DHPGG E+++ +  KDAT DF+
Sbjct: 1   MGGDGKVFTLSEVSQHSSAKDCWIVIDGKVYDVTKFLDDHPGGDEVILTSTGKDATDDFE 60

Query: 61  SVGHSETAQERMKKYQIGKIDPLTLPTEQKHVETTPHKPT-----KSLLSFSPLHVLIPL 120
            VGHS TA+  + +Y +G ID  T+P + K V  T  K        S      L  L+PL
Sbjct: 61  DVGHSSTAKAMLDEYYVGDIDTATVPVKAKFVPPTSTKAVATQDKSSDFVIKLLQFLVPL 120

Query: 121 LLFVLAIAFPY 127
           L+  LA    Y
Sbjct: 121 LILGLAFGIRY 131

[Close]

BLAST of Cp4.1LG17g04430 vs. TAIR10
Match: AT2G46650.1 (AT2G46650.1 cytochrome B5 isoform C)

HSP 1 Score: 100.1 bits (248), Expect = 1.0e-21
Identity = 51/131 (38.93%), Postives = 80/131 (61.07%), Query Frame = 1


Query: 5   SKVYTFDEVAKHNHEKDCWLIISGYVYDVTSYLPDHPGGGELLVLAVEKDATFDFKSVGH 64
           + + +F +VAKH  + DCW++I G VYD+++++ +HPGG  +L+    KDA+ DF+ V H
Sbjct: 2   ANLISFHDVAKHKCKNDCWILIHGKVYDISTFMDEHPGGDNVLLAVTGKDASIDFEDVNH 61

Query: 65  SETAQERMKKYQIGKIDPLTLPTEQKHV-----ETTPHKPTKSLLSFSPLHVLIPLLLFV 124
           S+ A+E MKKY IG +D  T+P  Q+++     E+T  + TK       L  LIPLL+  
Sbjct: 62  SKDAKELMKKYCIGDVDQSTVPVTQQYIPPWEKESTAAETTKEESGKKLLIYLIPLLILG 121

Query: 125 LAIAFPYLKIK 131
           +A A  +   K
Sbjct: 122 VAFALRFYNNK 132

[Close]

BLAST of Cp4.1LG17g04430 vs. TAIR10
Match: AT1G26340.1 (AT1G26340.1 cytochrome B5 isoform A)

HSP 1 Score: 99.8 bits (247), Expect = 1.4e-21
Identity = 49/130 (37.69%), Postives = 82/130 (63.08%), Query Frame = 1


Query: 1   MDSNSKVYTFDEVAKHNHEKDCWLIISGYVYDVTSYLPDHPGGGELLVLAVEKDATFDFK 60
           M + +K+Y+ +E A HN + DCW++I G VYDV+SY+ +HPGG ++L+    KDAT DF+
Sbjct: 1   MPTLTKLYSMEEAATHNKQDDCWVVIDGKVYDVSSYMDEHPGGDDVLLAVAGKDATDDFE 60

Query: 61  SVGHSETAQERMKKYQIGKIDPLTLP--TEQK-HVETTPHKPTKSLLSFSPLHVLIPLLL 120
             GHS+ A+E M+KY IG++D  +LP   E K + +  P    + L   +  + ++P+ +
Sbjct: 61  DAGHSKDARELMEKYFIGELDESSLPEIPELKIYKKDQPQDSVQKLFDLTKQYWVVPVSI 120

Query: 121 FVLAIAFPYL 128
             +++A   L
Sbjct: 121 ITISVAVSVL 130

[Close]

BLAST of Cp4.1LG17g04430 vs. NCBI nr
Match: gi|659119599|ref|XP_008459741.1| (PREDICTED: cytochrome b5 isoform A-like [Cucumis melo])

HSP 1 Score: 218.4 bits (555), Expect = 7.5e-54
Identity = 103/131 (78.63%), Postives = 118/131 (90.08%), Query Frame = 1


Query: 1   MDSNSKVYTFDEVAKHNHEKDCWLIISGYVYDVTSYLPDHPGGGELLVLAVEKDATFDFK 60
           MDSNSKVY+FDEVAKHNH+KDCWLIISGYVYDVTS+LPDHPGG ELL+LAVEKDATFDFK
Sbjct: 1   MDSNSKVYSFDEVAKHNHQKDCWLIISGYVYDVTSFLPDHPGGDELLLLAVEKDATFDFK 60

Query: 61  SVGHSETAQERMKKYQIGKIDPLTLPTEQKHVETTPHKPTKSLLSFSPLHVLIPLLLFVL 120
           SVGHSE AQE+MK Y+IGKID  TLP +Q +VE+TP++PT SL SFSP+HVL+P+LL  L
Sbjct: 61  SVGHSELAQEKMKMYRIGKIDMSTLPKKQNYVESTPYQPTPSLFSFSPIHVLLPILLLAL 120

Query: 121 AIAFPYLKIKA 132
           A AFPY K+KA
Sbjct: 121 AFAFPYFKLKA 131

[Close]

BLAST of Cp4.1LG17g04430 vs. NCBI nr
Match: gi|449445483|ref|XP_004140502.1| (PREDICTED: cytochrome b5 isoform E-like [Cucumis sativus])

HSP 1 Score: 210.3 bits (534), Expect = 2.0e-51
Identity = 100/131 (76.34%), Postives = 115/131 (87.79%), Query Frame = 1


Query: 1   MDSNSKVYTFDEVAKHNHEKDCWLIISGYVYDVTSYLPDHPGGGELLVLAVEKDATFDFK 60
           MDSNSKVY+FDEVAKHN +KDCWLIISGYVYDVTS+LPDHPGG ELL+LAVEKDATFDFK
Sbjct: 1   MDSNSKVYSFDEVAKHNQQKDCWLIISGYVYDVTSFLPDHPGGDELLLLAVEKDATFDFK 60

Query: 61  SVGHSETAQERMKKYQIGKIDPLTLPTEQKHVETTPHKPTKSLLSFSPLHVLIPLLLFVL 120
           SVGHSE A E+MK YQIGKID  TLP +QK+VE+ P++ T SL SFSP+H+L+P+LL  L
Sbjct: 61  SVGHSELAHEKMKMYQIGKIDMSTLPEKQKYVESAPYQRTPSLFSFSPIHLLLPILLLAL 120

Query: 121 AIAFPYLKIKA 132
           A AFPY K+KA
Sbjct: 121 AFAFPYFKLKA 131

[Close]

BLAST of Cp4.1LG17g04430 vs. NCBI nr
Match: gi|596010726|ref|XP_007218583.1| (hypothetical protein PRUPE_ppa013234mg [Prunus persica])

HSP 1 Score: 140.2 bits (352), Expect = 2.6e-30
Identity = 70/129 (54.26%), Postives = 91/129 (70.54%), Query Frame = 1


Query: 1   MDSNSKVYTFDEVAKHNHEKDCWLIISGYVYDVTSYLPDHPGGGELLVLAVEKDATFDFK 60
           M S+S+VY FDEVAKHNH+KDCW+IISG VYDVT +L DHPGG E+L+LAVEKDAT DF 
Sbjct: 1   MASDSQVYHFDEVAKHNHKKDCWIIISGKVYDVTPFLEDHPGGDEVLLLAVEKDATDDFN 60

Query: 61  SVGHSETAQERMKKYQIGKIDPLTLPTEQKH---VETTPHKPTKSLLSFSPLHVLIPLLL 120
            VGHS++A+E+M+K+ +GK+D  T+P +  +    +TTP     S      L  L+PLL+
Sbjct: 61  DVGHSDSAKEQMEKFYVGKVDTSTIPEQPSYKLPAQTTPPSGQSSGFVVKLLQFLLPLLI 120

Query: 121 FVLAIAFPY 127
              A A  Y
Sbjct: 121 LGAAFALQY 129

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BLAST of Cp4.1LG17g04430 vs. NCBI nr
Match: gi|645254539|ref|XP_008233085.1| (PREDICTED: cytochrome b5-like [Prunus mume])

HSP 1 Score: 138.3 bits (347), Expect = 9.8e-30
Identity = 69/129 (53.49%), Postives = 90/129 (69.77%), Query Frame = 1


Query: 1   MDSNSKVYTFDEVAKHNHEKDCWLIISGYVYDVTSYLPDHPGGGELLVLAVEKDATFDFK 60
           M S+S+VY FDEVAKHNH+KDCW+IISG VYDVT +L DHPGG E+L+LAVEKDAT DF 
Sbjct: 1   MASDSQVYHFDEVAKHNHKKDCWIIISGKVYDVTPFLEDHPGGDEVLLLAVEKDATDDFN 60

Query: 61  SVGHSETAQERMKKYQIGKIDPLTLPTEQKH---VETTPHKPTKSLLSFSPLHVLIPLLL 120
            VGHS++A+E+M+ + +GK+D  T+P +  +    +TTP     S      L  L+PLL+
Sbjct: 61  DVGHSDSAKEQMENFYVGKVDTSTIPEQPNYKLPAQTTPPSGQSSGFVVKLLQFLLPLLI 120

Query: 121 FVLAIAFPY 127
              A A  Y
Sbjct: 121 LGAAFALQY 129

[Close]

BLAST of Cp4.1LG17g04430 vs. NCBI nr
Match: gi|694400730|ref|XP_009375446.1| (PREDICTED: cytochrome b5-like [Pyrus x bretschneideri])

HSP 1 Score: 137.1 bits (344), Expect = 2.2e-29
Identity = 70/133 (52.63%), Postives = 90/133 (67.67%), Query Frame = 1


Query: 1   MDSNSKVYTFDEVAKHNHEKDCWLIISGYVYDVTSYLPDHPGGGELLVLAVEKDATFDFK 60
           M SNS+VY FDEVAKHNH+KDCW+IISG VYDVT +L +HPGG E+L+LAVEKDAT DF 
Sbjct: 1   MGSNSEVYLFDEVAKHNHKKDCWIIISGKVYDVTPFLEEHPGGDEVLLLAVEKDATDDFN 60

Query: 61  SVGHSETAQERMKKYQIGKIDPLTLPTEQKH---VETTPHKPTKSLLSFSPLHVLIPLLL 120
            VGHS++A+E+M+KY +GK+D  T+P +  +    +        S L    L  L+PLL+
Sbjct: 61  DVGHSDSAREQMEKYYVGKVDTSTIPAQPNYKLPPQAAAPAEQSSGLMVKLLQFLLPLLI 120

Query: 121 FVLAIAFPYLKIK 131
              A A  Y   K
Sbjct: 121 LGAAFALQYYNKK 133

The following BLAST results are available for this feature:

BLAST of Cp4.1LG17g04430 vs. Swiss-Prot


Match Name	E-value	Identity	Description
CYB5E_ARATH	5.6e-25	47.24	Cytochrome b5 isoform E OS=Arabidopsis thaliana GN=CYTB5-E PE=1 SV=2	[more]
CYB5S_TOBAC	9.5e-25	47.29	Cytochrome b5, seed isoform OS=Nicotiana tabacum PE=2 SV=1	[more]
CYB5_TOBAC	9.5e-25	46.51	Cytochrome b5 OS=Nicotiana tabacum PE=2 SV=1	[more]
CYB5_BRAOB	1.2e-24	47.24	Cytochrome b5 OS=Brassica oleracea var. botrytis GN=CYB5 PE=1 SV=1	[more]
CYB5B_ARATH	3.6e-24	43.31	Cytochrome b5 isoform B OS=Arabidopsis thaliana GN=CYTB5-B PE=1 SV=1	[more]

BLAST of Cp4.1LG17g04430 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KA20_CUCSA	1.4e-51	76.34	Uncharacterized protein OS=Cucumis sativus GN=Csa_6G107940 PE=3 SV=1	[more]
M5X125_PRUPE	1.8e-30	54.26	Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa013234mg PE=3 SV=1	[more]
W9RDL9_9ROSA	5.8e-29	53.08	Cytochrome b5, seed isoform OS=Morus notabilis GN=L484_023457 PE=3 SV=1	[more]
A0A151TY23_CAJCA	1.3e-28	51.15	Cytochrome b5 isoform 1 OS=Cajanus cajan GN=KK1_011257 PE=3 SV=1	[more]
A0A151SWJ2_CAJCA	8.3e-28	49.25	Cytochrome b5 OS=Cajanus cajan GN=KK1_014582 PE=3 SV=1	[more]

BLAST of Cp4.1LG17g04430 vs. TAIR10


Match Name	E-value	Identity	Description
AT5G53560.1	3.1e-26	47.24	cytochrome B5 isoform E	[more]
AT2G32720.1	2.0e-25	43.31	cytochrome B5 isoform B	[more]
AT5G48810.1	1.3e-24	42.75	cytochrome B5 isoform D	[more]
AT2G46650.1	1.0e-21	38.93	cytochrome B5 isoform C	[more]
AT1G26340.1	1.4e-21	37.69	cytochrome B5 isoform A	[more]

BLAST of Cp4.1LG17g04430 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|659119599\|ref\|XP_008459741.1\|	7.5e-54	78.63	PREDICTED: cytochrome b5 isoform A-like [Cucumis melo]	[more]
gi\|449445483\|ref\|XP_004140502.1\|	2.0e-51	76.34	PREDICTED: cytochrome b5 isoform E-like [Cucumis sativus]	[more]
gi\|596010726\|ref\|XP_007218583.1\|	2.6e-30	54.26	hypothetical protein PRUPE_ppa013234mg [Prunus persica]	[more]
gi\|645254539\|ref\|XP_008233085.1\|	9.8e-30	53.49	PREDICTED: cytochrome b5-like [Prunus mume]	[more]
gi\|694400730\|ref\|XP_009375446.1\|	2.2e-29	52.63	PREDICTED: cytochrome b5-like [Pyrus x bretschneideri]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **Molecular Function**
Term	Definition
GO:0020037	heme binding

Vocabulary: **INTERPRO**
Term	Definition
IPR018506	Cyt_B5_heme-BS
IPR001199	Cyt_B5-like_heme/steroid-bd

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0008150	biological_process
cellular_component	GO:0005575	cellular_component
cellular_component	GO:0009535	chloroplast thylakoid membrane
cellular_component	GO:0005783	endoplasmic reticulum
cellular_component	GO:0016021	integral component of membrane
cellular_component	GO:0005886	plasma membrane
cellular_component	GO:0005774	vacuolar membrane
molecular_function	GO:0020037	heme binding
molecular_function	GO:0046872	metal ion binding
molecular_function	GO:0016491	oxidoreductase activity

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cp4.1LG17g04430.1	Cp4.1LG17g04430.1	mRNA

InterPro

Analysis Name: InterPro Annotations of Cucurbita pepo
Date Performed: 2017-12-02


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001199	Cytochrome b5-like heme/steroid binding domain	PRINTS	PR00363	CYTOCHROMEB5	coord: 68..80 score: 6.6E-10coord: 55..62 score: 6.6E-10coord: 30..40 score: 6.6E-10coord: 40..54 score: 6.6
IPR001199	Cytochrome b5-like heme/steroid binding domain	GENE3D	G3DSA:3.10.120.10		coord: 5..82 score: 2.1
IPR001199	Cytochrome b5-like heme/steroid binding domain	PFAM	PF00173	Cyt-b5	coord: 10..80 score: 9.8
IPR001199	Cytochrome b5-like heme/steroid binding domain	SMART	SM01117	Cyt_b5_2	coord: 8..81 score: 3.0
IPR001199	Cytochrome b5-like heme/steroid binding domain	PROFILE	PS50255	CYTOCHROME_B5_2	coord: 5..81 score: 26
IPR001199	Cytochrome b5-like heme/steroid binding domain	unknown	SSF55856	Cytochrome b5-like heme/steroid binding domain	coord: 5..89 score: 1.96
IPR018506	Cytochrome b5, heme-binding site	PROSITE	PS00191	CYTOCHROME_B5_1	coord: 36..43 scor
None	No IPR available	PANTHER	PTHR19359	CYTOCHROME B5	coord: 1..123 score: 3.7
None	No IPR available	PANTHER	PTHR19359:SF22	CYTOCHROME B5 ISOFORM A	coord: 1..123 score: 3.7

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Cp4.1LG17g04430	Cucsa.120800	Cucumber (Gy14) v1	cgycpeB0295
Cp4.1LG17g04430	CmaCh08G008540	Cucurbita maxima (Rimu)	cmacpeB916
Cp4.1LG17g04430	Csa6G107940	Cucumber (Chinese Long) v2	cpecuB328
Cp4.1LG17g04430	MELO3C022247	Melon (DHL92) v3.5.1	cpemeB285
Cp4.1LG17g04430	ClCG06G002800	Watermelon (Charleston Gray)	cpewcgB299
Cp4.1LG17g04430	CSPI06G08590	Wild cucumber (PI 183967)	cpecpiB329
Cp4.1LG17g04430	Lsi09G016560	Bottle gourd (USVL1VR-Ls)	cpelsiB248
Cp4.1LG17g04430	MELO3C022247.2	Melon (DHL92) v3.6.1	cpemedB333
Cp4.1LG17g04430	CsaV3_6G008970	Cucumber (Chinese Long) v3	cpecucB0410
Cp4.1LG17g04430	Bhi12G001938	Wax gourd	cpewgoB0381
Cp4.1LG17g04430	CsGy6G008690	Cucumber (Gy14) v2	cgybcpeB790
Cp4.1LG17g04430	Carg13878	Silver-seed gourd	carcpeB0851

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Cp4.1LG17g04430	Cucurbita moschata (Rifu)	cmocpeB853