CSPI06G08590 (gene) Wild cucumber (PI 183967) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	CSPI06G08590
Type	gene
Organism	Cucumis sativus (Wild cucumber (PI 183967))
Description	Cytochrome b5
Location	Chr6 : 7220613 .. 7221850 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

GAATAAATTGTTGTACTTTTGCTCACAATATTTACTTCTTTTCTTCTGCTTCTTCTTATCTTCTTCTATTTAAGGTCTAAAATATTGAAACTCTGTTGAAGGGAAGGATTAGAAAAAGAAAAAGAAAAGTATTTGATAGAAAATGGATTCAAATTCAAAAGTGTATTCCTTTGATGAAGTTGCAAAGCACAACCAGCAGAAGGATTGTTGGCTTATAATCTCTGGATATGTATGTTCTTTATTTCCCTTTTCTTCTTGTTGAATAACTCTCTCTACCTAATTTACAAATATATTCATTGCTTGATTAATTAGTATTTCTATTACATATCATCATAATAAGATTTGGATTAATGTTAATGCGCATTCTGTTGGGTCTAAGTTTTAGAAGAGTGTGAGTGTTGTTGTGTTTATAGTTAGTTCTTGTCTGAAGTGGTTTTGGAGGATTGTGAAGCTAAATGTGTATTGTTTTTAGGTGTACGACGTGACTTCATTTTTGCCGGACCATCCAGGCGGCGATGAGCTTCTGCTACTTGCAGTGGGTGAGATGGCTACACACCCATTTCTAATCATACAAATCCATATCTCTACTTAATTACTTTTTCTAATGTTTTATATATGCACATTAACATGGTTTACTTACTATACATTTTACTCTTTATACTTTTACAAATCTTAAAATTAGTCTTCTCTTGTTTCCTTCAAATTAAGATTCATGCAACTTTTCAAAGGATGACCCTGTAATCACCATCCAATTTCAACATTTTGTGTGCTACAAAAACGGATTCAGCTTACGTTTTCTTGTATTTACTTTAACATTTTCTAACGACTAGTTGAAGTATTTTGTGGATTAATTAACATGATAGATTTGAATATATGCAGAAAAAGATGCAACATTTGATTTTAAGAGTGTTGGACATAGCGAACTAGCTCATGAGAAGATGAAAATGTATCAAATAGGGAAGATCGACATGTCGACTCTCCCTGAAAAACAAAAGTACGTGGAAAGTGCACCATATCAACGAACTCCAAGTCTATTTTCCTTCTCACCCATCCATATTCTACTCCCTATTTTGCTCTTAGCCTTGGCTTTTGCATTTCCCTACTTCAAACTTAAGGCCTAAAATTCATATTATTTTACATTTCTATTTTACTTTCCCACAAGTATTATTATAGCCTATGGGCTATAAAATAACAATTTCTAGCAACTTCGTGATTGAATCGTTCAATGGATTTCTACC

mRNA sequence

ATGGATTCAAATTCAAAAGTGTATTCCTTTGATGAAGTTGCAAAGCACAACCAGCAGAAGGATTGTTGGCTTATAATCTCTGGATATGTGTACGACGTGACTTCATTTTTGCCGGACCATCCAGGCGGCGATGAGCTTCTGCTACTTGCAGTGGAAAAAGATGCAACATTTGATTTTAAGAGTGTTGGACATAGCGAACTAGCTCATGAGAAGATGAAAATGTATCAAATAGGGAAGATCGACATGTCGACTCTCCCTGAAAAACAAAAGTACGTGGAAAGTGCACCATATCAACGAACTCCAAGTCTATTTTCCTTCTCACCCATCCATATTCTACTCCCTATTTTGCTCTTAGCCTTGGCTTTTGCATTTCCCTACTTCAAACTTAAGGCCTAA

Coding sequence (CDS)

ATGGATTCAAATTCAAAAGTGTATTCCTTTGATGAAGTTGCAAAGCACAACCAGCAGAAGGATTGTTGGCTTATAATCTCTGGATATGTGTACGACGTGACTTCATTTTTGCCGGACCATCCAGGCGGCGATGAGCTTCTGCTACTTGCAGTGGAAAAAGATGCAACATTTGATTTTAAGAGTGTTGGACATAGCGAACTAGCTCATGAGAAGATGAAAATGTATCAAATAGGGAAGATCGACATGTCGACTCTCCCTGAAAAACAAAAGTACGTGGAAAGTGCACCATATCAACGAACTCCAAGTCTATTTTCCTTCTCACCCATCCATATTCTACTCCCTATTTTGCTCTTAGCCTTGGCTTTTGCATTTCCCTACTTCAAACTTAAGGCCTAA

Homology

[Close]

BLAST of CSPI06G08590 vs. Swiss-Prot
Match: CYB5E_ARATH (Cytochrome b5 isoform E OS=Arabidopsis thaliana GN=CYTB5-E PE=1 SV=2)

HSP 1 Score: 129.4 bits (324), Expect = 2.9e-29
Identity = 70/136 (51.47%), Postives = 90/136 (66.18%), Query Frame = 1


Query: 1   MDSNSKVYSFDEVAKHNQQKDCWLIISGYVYDVTSFLPDHPGGDELLLLAVEKDATFDFK 60
           M S+ KV SF+EV+KHN+ KDCWLIISG VYDVT F+ DHPGGDE+LL +  KDAT DF+
Sbjct: 1   MSSDRKVLSFEEVSKHNKTKDCWLIISGKVYDVTPFMDDHPGGDEVLLSSTGKDATNDFE 60

Query: 61  SVGHSELAHEKMKMYQIGKIDMSTLPEKQKYVESAPYQ------RTPSLFSFSPIHILLP 120
            VGHS+ A + M  Y IG+ID S++P  + YV  AP Q      +TP  F    +  L+P
Sbjct: 61  DVGHSDTARDMMDKYFIGEIDSSSVPATRTYV--APQQPAYNQDKTPE-FIIKILQFLVP 120

Query: 121 ILLLALAFAFPYFKLK 131
           IL+L LA    ++  K
Sbjct: 121 ILILGLALVVRHYTKK 133

[Close]

BLAST of CSPI06G08590 vs. Swiss-Prot
Match: CYB5_BRAOB (Cytochrome b5 OS=Brassica oleracea var. botrytis GN=CYB5 PE=1 SV=1)

HSP 1 Score: 127.9 bits (320), Expect = 8.4e-29
Identity = 69/136 (50.74%), Postives = 88/136 (64.71%), Query Frame = 1


Query: 1   MDSNSKVYSFDEVAKHNQQKDCWLIISGYVYDVTSFLPDHPGGDELLLLAVEKDATFDFK 60
           M S  KV  F+EV++HN+ KDCWLIISG VYDVT F+ DHPGGDE+LL +  KDAT DF+
Sbjct: 1   MASEKKVLGFEEVSQHNKTKDCWLIISGKVYDVTPFMDDHPGGDEVLLSSTGKDATNDFE 60

Query: 61  SVGHSELAHEKMKMYQIGKIDMSTLPEKQKYVESAPYQ------RTPSLFSFSPIHILLP 120
            VGHS+ A + M+ Y IG+ID ST+P  + YV  AP Q      +TP  F    +  L+P
Sbjct: 61  DVGHSDTARDMMEKYYIGEIDSSTVPATRTYV--APVQPAYNQDKTPE-FMIKILQFLVP 120

Query: 121 ILLLALAFAFPYFKLK 131
           IL+L LA     +  K
Sbjct: 121 ILILGLALVVRQYTKK 133

[Close]

BLAST of CSPI06G08590 vs. Swiss-Prot
Match: CYB5_TOBAC (Cytochrome b5 OS=Nicotiana tabacum PE=2 SV=1)

HSP 1 Score: 121.3 bits (303), Expect = 7.8e-27
Identity = 61/130 (46.92%), Postives = 83/130 (63.85%), Query Frame = 1


Query: 1   MDSNSKVYSFDEVAKHNQQKDCWLIISGYVYDVTSFLPDHPGGDELLLLAVEKDATFDFK 60
           M   +KV++  EV++HN  KDCWL+ISG VYDVT FL DHPGGDE+LL A  KDAT DF+
Sbjct: 1   MGGETKVFTLAEVSQHNNAKDCWLVISGKVYDVTKFLDDHPGGDEVLLSATGKDATDDFE 60

Query: 61  SVGHSELAHEKMKMYQIGKIDMSTLPEKQKYV-ESAPY--QRTPSLFSFSPIHILLPILL 120
            VGHS  A   +  Y +G ID +T+P K KY   + P+  Q   S F    +  L+P+++
Sbjct: 61  DVGHSSSARAMLDEYYVGDIDSATIPTKTKYTPPNQPHYNQDKTSEFVVKLLQFLVPLII 120

Query: 121 LALAFAFPYF 128
           L +AF   ++
Sbjct: 121 LGVAFGIRFY 130

[Close]

BLAST of CSPI06G08590 vs. Swiss-Prot
Match: CYB5B_ARATH (Cytochrome b5 isoform B OS=Arabidopsis thaliana GN=CYTB5-B PE=1 SV=1)

HSP 1 Score: 120.2 bits (300), Expect = 1.7e-26
Identity = 58/124 (46.77%), Postives = 83/124 (66.94%), Query Frame = 1


Query: 1   MDSNSKVYSFDEVAKHNQQKDCWLIISGYVYDVTSFLPDHPGGDELLLLAVEKDATFDFK 60
           M   +K+++  EV++HNQ  DCW++I+G VY+VT FL DHPGGD++LL +  KDAT DF+
Sbjct: 1   MGDEAKIFTLSEVSEHNQAHDCWIVINGKVYNVTKFLEDHPGGDDVLLSSTGKDATDDFE 60

Query: 61  SVGHSELAHEKMKMYQIGKIDMSTLPEKQKYV-ESAPY--QRTPSLFSFSPIHILLPILL 120
            VGHSE A E M+ Y +G+ID +T+P+K KY     P+  Q   S F    +  L+P+ +
Sbjct: 61  DVGHSESAREMMEQYYVGEIDPTTIPKKVKYTPPKQPHYNQDKTSEFIIKLLQFLVPLAI 120

Query: 121 LALA 122
           L LA
Sbjct: 121 LGLA 124

[Close]

BLAST of CSPI06G08590 vs. Swiss-Prot
Match: CYB5_ORYSJ (Cytochrome b5 OS=Oryza sativa subsp. japonica GN=Os05g0108800 PE=2 SV=2)

HSP 1 Score: 119.8 bits (299), Expect = 2.3e-26
Identity = 62/126 (49.21%), Postives = 81/126 (64.29%), Query Frame = 1


Query: 2   DSNSKVYSFDEVAKHNQQKDCWLIISGYVYDVTSFLPDHPGGDELLLLAVEKDATFDFKS 61
           + N KVY+ +EVAKHN + DCWLII G VY+V+ FL DHPGGD++LL +  KDAT DF+ 
Sbjct: 3   NDNKKVYTLEEVAKHNSKDDCWLIIGGKVYNVSKFLEDHPGGDDVLLSSTGKDATDDFED 62

Query: 62  VGHSELAHEKMKMYQIGKIDMSTLPEKQKYV-ESAPY---QRTPSLFSFSPIHILLPILL 121
           VGHS  A   M  Y +G ID ST+P + KYV    P+    +TP  F    +  L+P+ +
Sbjct: 63  VGHSTTARAMMDEYYVGDIDTSTIPARTKYVPPKQPHYNQDKTPE-FIIKILQFLVPLAI 122

Query: 122 LALAFA 124
           L LA A
Sbjct: 123 LGLAVA 127

[Close]

BLAST of CSPI06G08590 vs. TrEMBL
Match: A0A0A0KA20_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_6G107940 PE=3 SV=1)

HSP 1 Score: 266.2 bits (679), Expect = 2.2e-68
Identity = 130/131 (99.24%), Postives = 131/131 (100.00%), Query Frame = 1


Query: 1   MDSNSKVYSFDEVAKHNQQKDCWLIISGYVYDVTSFLPDHPGGDELLLLAVEKDATFDFK 60
           MDSNSKVYSFDEVAKHNQQKDCWLIISGYVYDVTSFLPDHPGGDELLLLAVEKDATFDFK
Sbjct: 1   MDSNSKVYSFDEVAKHNQQKDCWLIISGYVYDVTSFLPDHPGGDELLLLAVEKDATFDFK 60

Query: 61  SVGHSELAHEKMKMYQIGKIDMSTLPEKQKYVESAPYQRTPSLFSFSPIHILLPILLLAL 120
           SVGHSELAHEKMKMYQIGKIDMSTLPEKQKYVESAPYQRTPSLFSFSPIH+LLPILLLAL
Sbjct: 61  SVGHSELAHEKMKMYQIGKIDMSTLPEKQKYVESAPYQRTPSLFSFSPIHLLLPILLLAL 120

Query: 121 AFAFPYFKLKA 132
           AFAFPYFKLKA
Sbjct: 121 AFAFPYFKLKA 131

[Close]

BLAST of CSPI06G08590 vs. TrEMBL
Match: M5X125_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa013234mg PE=3 SV=1)

HSP 1 Score: 151.0 bits (380), Expect = 1.0e-33
Identity = 78/135 (57.78%), Postives = 95/135 (70.37%), Query Frame = 1


Query: 1   MDSNSKVYSFDEVAKHNQQKDCWLIISGYVYDVTSFLPDHPGGDELLLLAVEKDATFDFK 60
           M S+S+VY FDEVAKHN +KDCW+IISG VYDVT FL DHPGGDE+LLLAVEKDAT DF 
Sbjct: 1   MASDSQVYHFDEVAKHNHKKDCWIIISGKVYDVTPFLEDHPGGDEVLLLAVEKDATDDFN 60

Query: 61  SVGHSELAHEKMKMYQIGKIDMSTLPEKQKYVESAPYQRTP-----SLFSFSPIHILLPI 120
            VGHS+ A E+M+ + +GK+D ST+PE+  Y    P Q TP     S F    +  LLP+
Sbjct: 61  DVGHSDSAKEQMEKFYVGKVDTSTIPEQPSY--KLPAQTTPPSGQSSGFVVKLLQFLLPL 120

Query: 121 LLLALAFAFPYFKLK 131
           L+L  AFA  Y+  K
Sbjct: 121 LILGAAFALQYYGKK 133

[Close]

BLAST of CSPI06G08590 vs. TrEMBL
Match: W9QM38_9ROSA (Cytochrome b5 isoform 1 OS=Morus notabilis GN=L484_001660 PE=3 SV=1)

HSP 1 Score: 145.6 bits (366), Expect = 4.3e-32
Identity = 76/134 (56.72%), Postives = 97/134 (72.39%), Query Frame = 1


Query: 1   MDSNSKVYSFDEVAKHNQQKDCWLIISGYVYDVTSFLPDHPGGDELLLLAVEKDATFDFK 60
           M S+SKV+ F+EVAKHN+ KDCWLIISG VYDVT+F+ DHPGGDE+LL A  KDAT DF+
Sbjct: 1   MASDSKVHVFEEVAKHNKTKDCWLIISGKVYDVTTFMDDHPGGDEVLLSATGKDATNDFE 60

Query: 61  SVGHSELAHEKMKMYQIGKIDMSTLPEKQKYV--ESAPY--QRTPSLFSFSPIHILLPIL 120
            VGHS+ A + MK Y IG+ID ST+P K+ Y+  + APY   +TP  F    +  L+P+L
Sbjct: 61  DVGHSDSARDMMKKYYIGEIDSSTVPLKRTYIPPQQAPYNPDKTPE-FVIKILQFLVPLL 120

Query: 121 LLALAFAFPYFKLK 131
           +L LAFA  +F  K
Sbjct: 121 ILGLAFAVRHFTKK 133

[Close]

BLAST of CSPI06G08590 vs. TrEMBL
Match: W9RDL9_9ROSA (Cytochrome b5, seed isoform OS=Morus notabilis GN=L484_023457 PE=3 SV=1)

HSP 1 Score: 145.6 bits (366), Expect = 4.3e-32
Identity = 74/133 (55.64%), Postives = 96/133 (72.18%), Query Frame = 1


Query: 1   MDSNSKVYSFDEVAKHNQQKDCWLIISGYVYDVTSFLPDHPGGDELLLLAVEKDATFDFK 60
           M  +SKV++FDEVAKHN +KDCW+IISG VY+VT FL +HPGGDE+LLLA EKDAT DF+
Sbjct: 1   MARDSKVFAFDEVAKHNHKKDCWIIISGKVYNVTEFLEEHPGGDEVLLLAAEKDATDDFE 60

Query: 61  SVGHSELAHEKMKMYQIGKIDMSTLPEKQKYVESAPYQRTPSLFSFSP------IHILLP 120
            VGHS+ A E M+ Y +GK+D+STLPEK  Y    P  + PS  + S       +  LLP
Sbjct: 61  DVGHSDTAREMMEKYYVGKVDVSTLPEKPSY---KPPPQPPSQSNQSSGGSVKILQFLLP 120

Query: 121 ILLLALAFAFPYF 128
           +L+LA+AFA  ++
Sbjct: 121 LLILAIAFALHFY 130

[Close]

BLAST of CSPI06G08590 vs. TrEMBL
Match: A0A151TY23_CAJCA (Cytochrome b5 isoform 1 OS=Cajanus cajan GN=KK1_011257 PE=3 SV=1)

HSP 1 Score: 141.0 bits (354), Expect = 1.1e-30
Identity = 70/135 (51.85%), Postives = 90/135 (66.67%), Query Frame = 1


Query: 1   MDSNSKVYSFDEVAKHNQQKDCWLIISGYVYDVTSFLPDHPGGDELLLLAVEKDATFDFK 60
           M  NSK  +F+EVAKHN +KDCW+I+ G VYD+T FL DHPGGDE+L+ A EKDAT DF+
Sbjct: 1   MALNSKTLTFEEVAKHNHKKDCWIIVHGKVYDITPFLDDHPGGDEVLVAATEKDATIDFE 60

Query: 61  SVGHSELAHEKMKMYQIGKIDMSTLPEKQKYVESAPYQ-----RTPSLFSFSPIHILLPI 120
            VGHS+ A E M+ Y IGK+D +TLP K  +++  P Q        S F    +  LLP+
Sbjct: 61  DVGHSDSATEMMERYLIGKVDTATLPAKVNHIQQPPTQAHGVGNQSSGFVVKMLQFLLPL 120

Query: 121 LLLALAFAFPYFKLK 131
           LLL LAFA  ++  K
Sbjct: 121 LLLGLAFALQHYGKK 135

[Close]

BLAST of CSPI06G08590 vs. TAIR10
Match: AT5G53560.1 (AT5G53560.1 cytochrome B5 isoform E)

HSP 1 Score: 129.4 bits (324), Expect = 1.6e-30
Identity = 70/136 (51.47%), Postives = 90/136 (66.18%), Query Frame = 1


Query: 1   MDSNSKVYSFDEVAKHNQQKDCWLIISGYVYDVTSFLPDHPGGDELLLLAVEKDATFDFK 60
           M S+ KV SF+EV+KHN+ KDCWLIISG VYDVT F+ DHPGGDE+LL +  KDAT DF+
Sbjct: 1   MSSDRKVLSFEEVSKHNKTKDCWLIISGKVYDVTPFMDDHPGGDEVLLSSTGKDATNDFE 60

Query: 61  SVGHSELAHEKMKMYQIGKIDMSTLPEKQKYVESAPYQ------RTPSLFSFSPIHILLP 120
            VGHS+ A + M  Y IG+ID S++P  + YV  AP Q      +TP  F    +  L+P
Sbjct: 61  DVGHSDTARDMMDKYFIGEIDSSSVPATRTYV--APQQPAYNQDKTPE-FIIKILQFLVP 120

Query: 121 ILLLALAFAFPYFKLK 131
           IL+L LA    ++  K
Sbjct: 121 ILILGLALVVRHYTKK 133

[Close]

BLAST of CSPI06G08590 vs. TAIR10
Match: AT2G32720.1 (AT2G32720.1 cytochrome B5 isoform B)

HSP 1 Score: 120.2 bits (300), Expect = 9.8e-28
Identity = 58/124 (46.77%), Postives = 83/124 (66.94%), Query Frame = 1


Query: 1   MDSNSKVYSFDEVAKHNQQKDCWLIISGYVYDVTSFLPDHPGGDELLLLAVEKDATFDFK 60
           M   +K+++  EV++HNQ  DCW++I+G VY+VT FL DHPGGD++LL +  KDAT DF+
Sbjct: 1   MGDEAKIFTLSEVSEHNQAHDCWIVINGKVYNVTKFLEDHPGGDDVLLSSTGKDATDDFE 60

Query: 61  SVGHSELAHEKMKMYQIGKIDMSTLPEKQKYV-ESAPY--QRTPSLFSFSPIHILLPILL 120
            VGHSE A E M+ Y +G+ID +T+P+K KY     P+  Q   S F    +  L+P+ +
Sbjct: 61  DVGHSESAREMMEQYYVGEIDPTTIPKKVKYTPPKQPHYNQDKTSEFIIKLLQFLVPLAI 120

Query: 121 LALA 122
           L LA
Sbjct: 121 LGLA 124

[Close]

BLAST of CSPI06G08590 vs. TAIR10
Match: AT5G48810.1 (AT5G48810.1 cytochrome B5 isoform D)

HSP 1 Score: 119.4 bits (298), Expect = 1.7e-27
Identity = 61/137 (44.53%), Postives = 85/137 (62.04%), Query Frame = 1


Query: 1   MDSNSKVYSFDEVAKHNQQKDCWLIISGYVYDVTSFLPDHPGGDELLLLAVEKDATFDFK 60
           M  + KV++  EV++H+  KDCW++I G VYDVT FL DHPGGDE++L +  KDAT DF+
Sbjct: 1   MGGDGKVFTLSEVSQHSSAKDCWIVIDGKVYDVTKFLDDHPGGDEVILTSTGKDATDDFE 60

Query: 61  SVGHSELAHEKMKMYQIGKIDMSTLPEKQKYV-----ESAPYQRTPSLFSFSPIHILLPI 120
            VGHS  A   +  Y +G ID +T+P K K+V     ++   Q   S F    +  L+P+
Sbjct: 61  DVGHSSTAKAMLDEYYVGDIDTATVPVKAKFVPPTSTKAVATQDKSSDFVIKLLQFLVPL 120

Query: 121 LLLALAFAFPYF-KLKA 132
           L+L LAF   Y+ K KA
Sbjct: 121 LILGLAFGIRYYTKTKA 137

[Close]

BLAST of CSPI06G08590 vs. TAIR10
Match: AT1G26340.1 (AT1G26340.1 cytochrome B5 isoform A)

HSP 1 Score: 110.9 bits (276), Expect = 6.0e-25
Identity = 52/126 (41.27%), Postives = 82/126 (65.08%), Query Frame = 1


Query: 1   MDSNSKVYSFDEVAKHNQQKDCWLIISGYVYDVTSFLPDHPGGDELLLLAVEKDATFDFK 60
           M + +K+YS +E A HN+Q DCW++I G VYDV+S++ +HPGGD++LL    KDAT DF+
Sbjct: 1   MPTLTKLYSMEEAATHNKQDDCWVVIDGKVYDVSSYMDEHPGGDDVLLAVAGKDATDDFE 60

Query: 61  SVGHSELAHEKMKMYQIGKIDMSTLPEKQK---YVESAPYQRTPSLFSFSPIHILLPILL 120
             GHS+ A E M+ Y IG++D S+LPE  +   Y +  P      LF  +  + ++P+ +
Sbjct: 61  DAGHSKDARELMEKYFIGELDESSLPEIPELKIYKKDQPQDSVQKLFDLTKQYWVVPVSI 120

Query: 121 LALAFA 124
           + ++ A
Sbjct: 121 ITISVA 126

[Close]

BLAST of CSPI06G08590 vs. TAIR10
Match: AT2G46650.1 (AT2G46650.1 cytochrome B5 isoform C)

HSP 1 Score: 109.8 bits (273), Expect = 1.3e-24
Identity = 54/131 (41.22%), Postives = 81/131 (61.83%), Query Frame = 1


Query: 5   SKVYSFDEVAKHNQQKDCWLIISGYVYDVTSFLPDHPGGDELLLLAVEKDATFDFKSVGH 64
           + + SF +VAKH  + DCW++I G VYD+++F+ +HPGGD +LL    KDA+ DF+ V H
Sbjct: 2   ANLISFHDVAKHKCKNDCWILIHGKVYDISTFMDEHPGGDNVLLAVTGKDASIDFEDVNH 61

Query: 65  SELAHEKMKMYQIGKIDMSTLPEKQKYV-----ESAPYQRTPSLFSFSPIHILLPILLLA 124
           S+ A E MK Y IG +D ST+P  Q+Y+     ES   + T        +  L+P+L+L 
Sbjct: 62  SKDAKELMKKYCIGDVDQSTVPVTQQYIPPWEKESTAAETTKEESGKKLLIYLIPLLILG 121

Query: 125 LAFAFPYFKLK 131
           +AFA  ++  K
Sbjct: 122 VAFALRFYNNK 132

[Close]

BLAST of CSPI06G08590 vs. NCBI nr
Match: gi|449445483|ref|XP_004140502.1| (PREDICTED: cytochrome b5 isoform E-like [Cucumis sativus])

HSP 1 Score: 266.2 bits (679), Expect = 3.1e-68
Identity = 130/131 (99.24%), Postives = 131/131 (100.00%), Query Frame = 1


Query: 1   MDSNSKVYSFDEVAKHNQQKDCWLIISGYVYDVTSFLPDHPGGDELLLLAVEKDATFDFK 60
           MDSNSKVYSFDEVAKHNQQKDCWLIISGYVYDVTSFLPDHPGGDELLLLAVEKDATFDFK
Sbjct: 1   MDSNSKVYSFDEVAKHNQQKDCWLIISGYVYDVTSFLPDHPGGDELLLLAVEKDATFDFK 60

Query: 61  SVGHSELAHEKMKMYQIGKIDMSTLPEKQKYVESAPYQRTPSLFSFSPIHILLPILLLAL 120
           SVGHSELAHEKMKMYQIGKIDMSTLPEKQKYVESAPYQRTPSLFSFSPIH+LLPILLLAL
Sbjct: 61  SVGHSELAHEKMKMYQIGKIDMSTLPEKQKYVESAPYQRTPSLFSFSPIHLLLPILLLAL 120

Query: 121 AFAFPYFKLKA 132
           AFAFPYFKLKA
Sbjct: 121 AFAFPYFKLKA 131

[Close]

BLAST of CSPI06G08590 vs. NCBI nr
Match: gi|659119599|ref|XP_008459741.1| (PREDICTED: cytochrome b5 isoform A-like [Cucumis melo])

HSP 1 Score: 252.3 bits (643), Expect = 4.7e-64
Identity = 123/131 (93.89%), Postives = 126/131 (96.18%), Query Frame = 1


Query: 1   MDSNSKVYSFDEVAKHNQQKDCWLIISGYVYDVTSFLPDHPGGDELLLLAVEKDATFDFK 60
           MDSNSKVYSFDEVAKHN QKDCWLIISGYVYDVTSFLPDHPGGDELLLLAVEKDATFDFK
Sbjct: 1   MDSNSKVYSFDEVAKHNHQKDCWLIISGYVYDVTSFLPDHPGGDELLLLAVEKDATFDFK 60

Query: 61  SVGHSELAHEKMKMYQIGKIDMSTLPEKQKYVESAPYQRTPSLFSFSPIHILLPILLLAL 120
           SVGHSELA EKMKMY+IGKIDMSTLP+KQ YVES PYQ TPSLFSFSPIH+LLPILLLAL
Sbjct: 61  SVGHSELAQEKMKMYRIGKIDMSTLPKKQNYVESTPYQPTPSLFSFSPIHVLLPILLLAL 120

Query: 121 AFAFPYFKLKA 132
           AFAFPYFKLKA
Sbjct: 121 AFAFPYFKLKA 131

[Close]

BLAST of CSPI06G08590 vs. NCBI nr
Match: gi|596010726|ref|XP_007218583.1| (hypothetical protein PRUPE_ppa013234mg [Prunus persica])

HSP 1 Score: 151.0 bits (380), Expect = 1.5e-33
Identity = 78/135 (57.78%), Postives = 95/135 (70.37%), Query Frame = 1


Query: 1   MDSNSKVYSFDEVAKHNQQKDCWLIISGYVYDVTSFLPDHPGGDELLLLAVEKDATFDFK 60
           M S+S+VY FDEVAKHN +KDCW+IISG VYDVT FL DHPGGDE+LLLAVEKDAT DF 
Sbjct: 1   MASDSQVYHFDEVAKHNHKKDCWIIISGKVYDVTPFLEDHPGGDEVLLLAVEKDATDDFN 60

Query: 61  SVGHSELAHEKMKMYQIGKIDMSTLPEKQKYVESAPYQRTP-----SLFSFSPIHILLPI 120
            VGHS+ A E+M+ + +GK+D ST+PE+  Y    P Q TP     S F    +  LLP+
Sbjct: 61  DVGHSDSAKEQMEKFYVGKVDTSTIPEQPSY--KLPAQTTPPSGQSSGFVVKLLQFLLPL 120

Query: 121 LLLALAFAFPYFKLK 131
           L+L  AFA  Y+  K
Sbjct: 121 LILGAAFALQYYGKK 133

[Close]

BLAST of CSPI06G08590 vs. NCBI nr
Match: gi|645254539|ref|XP_008233085.1| (PREDICTED: cytochrome b5-like [Prunus mume])

HSP 1 Score: 150.6 bits (379), Expect = 1.9e-33
Identity = 78/135 (57.78%), Postives = 95/135 (70.37%), Query Frame = 1


Query: 1   MDSNSKVYSFDEVAKHNQQKDCWLIISGYVYDVTSFLPDHPGGDELLLLAVEKDATFDFK 60
           M S+S+VY FDEVAKHN +KDCW+IISG VYDVT FL DHPGGDE+LLLAVEKDAT DF 
Sbjct: 1   MASDSQVYHFDEVAKHNHKKDCWIIISGKVYDVTPFLEDHPGGDEVLLLAVEKDATDDFN 60

Query: 61  SVGHSELAHEKMKMYQIGKIDMSTLPEKQKYVESAPYQRTP-----SLFSFSPIHILLPI 120
            VGHS+ A E+M+ + +GK+D ST+PE+  Y    P Q TP     S F    +  LLP+
Sbjct: 61  DVGHSDSAKEQMENFYVGKVDTSTIPEQPNY--KLPAQTTPPSGQSSGFVVKLLQFLLPL 120

Query: 121 LLLALAFAFPYFKLK 131
           L+L  AFA  Y+  K
Sbjct: 121 LILGAAFALQYYGKK 133

[Close]

BLAST of CSPI06G08590 vs. NCBI nr
Match: gi|694400730|ref|XP_009375446.1| (PREDICTED: cytochrome b5-like [Pyrus x bretschneideri])

HSP 1 Score: 148.7 bits (374), Expect = 7.3e-33
Identity = 75/134 (55.97%), Postives = 95/134 (70.90%), Query Frame = 1


Query: 1   MDSNSKVYSFDEVAKHNQQKDCWLIISGYVYDVTSFLPDHPGGDELLLLAVEKDATFDFK 60
           M SNS+VY FDEVAKHN +KDCW+IISG VYDVT FL +HPGGDE+LLLAVEKDAT DF 
Sbjct: 1   MGSNSEVYLFDEVAKHNHKKDCWIIISGKVYDVTPFLEEHPGGDEVLLLAVEKDATDDFN 60

Query: 61  SVGHSELAHEKMKMYQIGKIDMSTLPEKQKY----VESAPYQRTPSLFSFSPIHILLPIL 120
            VGHS+ A E+M+ Y +GK+D ST+P +  Y      +AP +++  L     +  LLP+L
Sbjct: 61  DVGHSDSAREQMEKYYVGKVDTSTIPAQPNYKLPPQAAAPAEQSSGLM-VKLLQFLLPLL 120

Query: 121 LLALAFAFPYFKLK 131
           +L  AFA  Y+  K
Sbjct: 121 ILGAAFALQYYNKK 133

The following BLAST results are available for this feature:

BLAST of CSPI06G08590 vs. Swiss-Prot


Match Name	E-value	Identity	Description
CYB5E_ARATH	2.9e-29	51.47	Cytochrome b5 isoform E OS=Arabidopsis thaliana GN=CYTB5-E PE=1 SV=2	[more]
CYB5_BRAOB	8.4e-29	50.74	Cytochrome b5 OS=Brassica oleracea var. botrytis GN=CYB5 PE=1 SV=1	[more]
CYB5_TOBAC	7.8e-27	46.92	Cytochrome b5 OS=Nicotiana tabacum PE=2 SV=1	[more]
CYB5B_ARATH	1.7e-26	46.77	Cytochrome b5 isoform B OS=Arabidopsis thaliana GN=CYTB5-B PE=1 SV=1	[more]
CYB5_ORYSJ	2.3e-26	49.21	Cytochrome b5 OS=Oryza sativa subsp. japonica GN=Os05g0108800 PE=2 SV=2	[more]

BLAST of CSPI06G08590 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KA20_CUCSA	2.2e-68	99.24	Uncharacterized protein OS=Cucumis sativus GN=Csa_6G107940 PE=3 SV=1	[more]
M5X125_PRUPE	1.0e-33	57.78	Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa013234mg PE=3 SV=1	[more]
W9QM38_9ROSA	4.3e-32	56.72	Cytochrome b5 isoform 1 OS=Morus notabilis GN=L484_001660 PE=3 SV=1	[more]
W9RDL9_9ROSA	4.3e-32	55.64	Cytochrome b5, seed isoform OS=Morus notabilis GN=L484_023457 PE=3 SV=1	[more]
A0A151TY23_CAJCA	1.1e-30	51.85	Cytochrome b5 isoform 1 OS=Cajanus cajan GN=KK1_011257 PE=3 SV=1	[more]

BLAST of CSPI06G08590 vs. TAIR10


Match Name	E-value	Identity	Description
AT5G53560.1	1.6e-30	51.47	cytochrome B5 isoform E	[more]
AT2G32720.1	9.8e-28	46.77	cytochrome B5 isoform B	[more]
AT5G48810.1	1.7e-27	44.53	cytochrome B5 isoform D	[more]
AT1G26340.1	6.0e-25	41.27	cytochrome B5 isoform A	[more]
AT2G46650.1	1.3e-24	41.22	cytochrome B5 isoform C	[more]

BLAST of CSPI06G08590 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|449445483\|ref\|XP_004140502.1\|	3.1e-68	99.24	PREDICTED: cytochrome b5 isoform E-like [Cucumis sativus]	[more]
gi\|659119599\|ref\|XP_008459741.1\|	4.7e-64	93.89	PREDICTED: cytochrome b5 isoform A-like [Cucumis melo]	[more]
gi\|596010726\|ref\|XP_007218583.1\|	1.5e-33	57.78	hypothetical protein PRUPE_ppa013234mg [Prunus persica]	[more]
gi\|645254539\|ref\|XP_008233085.1\|	1.9e-33	57.78	PREDICTED: cytochrome b5-like [Prunus mume]	[more]
gi\|694400730\|ref\|XP_009375446.1\|	7.3e-33	55.97	PREDICTED: cytochrome b5-like [Pyrus x bretschneideri]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR001199	Cyt_B5-like_heme/steroid-bd
IPR018506	Cyt_B5_heme-BS

Vocabulary: **Molecular Function**
Term	Definition
GO:0020037	heme binding

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0008150	biological_process
cellular_component	GO:0005575	cellular_component
cellular_component	GO:0009535	chloroplast thylakoid membrane
cellular_component	GO:0005783	endoplasmic reticulum
cellular_component	GO:0016021	integral component of membrane
cellular_component	GO:0005886	plasma membrane
cellular_component	GO:0005774	vacuolar membrane
molecular_function	GO:0020037	heme binding
molecular_function	GO:0046872	metal ion binding
molecular_function	GO:0016491	oxidoreductase activity

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI06G08590.1	CSPI06G08590.1	mRNA

InterPro

Analysis Name: InterPro Annotations of cucumber (PI183967)
Date Performed: 2017-01-17


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001199	Cytochrome b5-like heme/steroid binding domain	PRINTS	PR00363	CYTOCHROMEB5	coord: 30..40 score: 4.9E-10coord: 55..62 score: 4.9E-10coord: 40..54 score: 4.9E-10coord: 68..80 score: 4.9
IPR001199	Cytochrome b5-like heme/steroid binding domain	GENE3D	G3DSA:3.10.120.10		coord: 4..88 score: 2.1
IPR001199	Cytochrome b5-like heme/steroid binding domain	PFAM	PF00173	Cyt-b5	coord: 11..80 score: 5.8
IPR001199	Cytochrome b5-like heme/steroid binding domain	SMART	SM01117	Cyt_b5_2	coord: 8..81 score: 9.1
IPR001199	Cytochrome b5-like heme/steroid binding domain	PROFILE	PS50255	CYTOCHROME_B5_2	coord: 5..81 score: 26
IPR001199	Cytochrome b5-like heme/steroid binding domain	unknown	SSF55856	Cytochrome b5-like heme/steroid binding domain	coord: 5..88 score: 2.36
IPR018506	Cytochrome b5, heme-binding site	PROSITE	PS00191	CYTOCHROME_B5_1	coord: 36..43 scor
None	No IPR available	PANTHER	PTHR19359	CYTOCHROME B5	coord: 1..123 score: 2.7
None	No IPR available	PANTHER	PTHR19359:SF22	CYTOCHROME B5 ISOFORM A	coord: 1..123 score: 2.7

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
CSPI06G08590	Csa6G107940	Cucumber (Chinese Long) v2	cpicuB314
CSPI06G08590	MELO3C022247	Melon (DHL92) v3.5.1	cpimeB436
CSPI06G08590	ClCG06G002800	Watermelon (Charleston Gray)	cpiwcgB503
CSPI06G08590	Lsi09G016560	Bottle gourd (USVL1VR-Ls)	cpilsiB401
CSPI06G08590	MELO3C022247.2	Melon (DHL92) v3.6.1	cpimedB435
CSPI06G08590	CsaV3_6G008970	Cucumber (Chinese Long) v3	cpicucB364
CSPI06G08590	Cla97C06G112170	Watermelon (97103) v2	cpiwmbB495
CSPI06G08590	Bhi12G001938	Wax gourd	cpiwgoB549
CSPI06G08590	Cucsa.120800	Cucumber (Gy14) v1	cgycpiB156
CSPI06G08590	CmaCh08G008540	Cucurbita maxima (Rimu)	cmacpiB918
CSPI06G08590	Cp4.1LG17g04430	Cucurbita pepo (Zucchini)	cpecpiB329
CSPI06G08590	CsGy6G008690	Cucumber (Gy14) v2	cgybcpiB288
CSPI06G08590	Carg13878	Silver-seed gourd	carcpiB0637

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
CSPI06G08590	Cucurbita maxima (Rimu)	cmacpiB383
CSPI06G08590	Cucurbita maxima (Rimu)	cmacpiB932
CSPI06G08590	Cucurbita moschata (Rifu)	cmocpiB372
CSPI06G08590	Cucurbita moschata (Rifu)	cmocpiB911
CSPI06G08590	Watermelon (97103) v1	cpiwmB563
CSPI06G08590	Cucurbita pepo (Zucchini)	cpecpiB154
CSPI06G08590	Cucurbita pepo (Zucchini)	cpecpiB332
CSPI06G08590	Bottle gourd (USVL1VR-Ls)	cpilsiB404
CSPI06G08590	Silver-seed gourd	carcpiB0097
CSPI06G08590	Silver-seed gourd	carcpiB0936