Cp4.1LG14g04000 (gene) Cucurbita pepo (Zucchini)

BLAST of Cp4.1LG14g04000 vs. Swiss-Prot
Match: NDC80_CHICK (Kinetochore protein NDC80 homolog OS=Gallus gallus GN=NDC80 PE=1 SV=1)

HSP 1 Score: 102.1 bits (253), Expect = 2.1e-20
Identity = 121/530 (22.83%), Postives = 230/530 (43.40%), Query Frame = 1

Query: 75  HSFNLTFKPFSPPSLKDLTETIHLL--LQCLDYH--PTKLEDDLPIVLKSLGYPFKINKG 134
           ++ N+T K    PS+KD  +    +    C  Y    +K E+++P V K LGYPF ++K 
Sbjct: 111 YAHNVTIKSLQSPSVKDFIKIFTFIYGFLCPSYELPDSKFEEEIPRVFKELGYPFPLSKS 170

Query: 135 ILKTPPAPHQWYPILALLHWLVQ-VAVY------------NYNLANDSDSFVSSNDVNV- 194
            + T  APH W  I+A L WL     +Y              N   ++D  +  N + + 
Sbjct: 171 SMYTVGAPHTWPQIVAALIWLTDCFKLYAAMRENPSSFDDGQNWGGETDDGIVHNKLFMD 230

Query: 195 YVLNSYVQYIRGDDDSVEALDQEFMERLKKGRDDAVESLNGLEMEVKELEAKAEAL---- 254
           YV+  Y  +++G  D+ E LD E   +LK   +     + GL  E K L  +   L    
Sbjct: 231 YVVKCYEHFMKG-GDTYEELDAEVQSKLKDLFNVDEFKIEGLAAENKRLHEEIARLEKER 290

Query: 255 RSDPSQREVLEKQQTVLEEDVKKFHTMIAGYSEKMQEMKKILEEKEKELEVKVQENKKIC 314
            S+P +   L K ++  + DV+K+   +A        + + ++   +E+E    E + + 
Sbjct: 291 ESEPDRLVSLRKLRSSFQADVQKYQAYLANLESHTTILDQKMKSVNEEVETTEMEVEAMK 350

Query: 315 EENEELKMRVESQMFNARDVERMRRELQAVERDIGDAEVSRNSWEEKTWDIDATVGNKFK 374
           +EN  L+   ++Q ++  D+ER+  E   +++ I        + E + W+ +       +
Sbjct: 351 QENARLQHIFDNQKYSVADIERINHERNELQQTINKLTKELEAEEHQLWNEELKYARNKE 410

Query: 375 ELEAMARECNQAMRRLKL-------DNCHQYFLNAKGSTPADVMGVDYKTTIKPAL---- 434
            +E    E ++  R+LKL          H + ++       + + V Y+T IK  L    
Sbjct: 411 AIETQLAEYHKLARKLKLIPISAENSKGHDFEIHFNPEAGPNCL-VKYRTQIKAPLMEIV 470

Query: 435 DSFAEDIKKSSMTKLEDLISLRQQSSENTAKIESRRSHIVSLQSHIEEVETQVNSLKNEI 494
           +   E+I+K++  K+    SL     +  A +  ++S + +L+   E+++   +    E 
Sbjct: 471 NQTEEEIRKATQRKM----SLEDTLEQVNAMVAEKKSSVKTLKEEAEKLDDLYHQKLKEA 530

Query: 495 RDYTLRCSTESKKMVEDVQLEAHSIDVVESEAGEILKNAKLKHEEAIKQGEEEILICARE 554
            +   +C+ E + + +  QL    ++   SEA   L + + +++  ++   EE       
Sbjct: 531 EEEEQKCANELELLEKHKQLLESGVNEGLSEATNELHDIQRQYQIVMQTTTEESRKAGDN 590

Query: 555 L-------VACVDSVSQYKEFVRSKISD-----MRSNLSKTAVGISEAYK 560
           L          V S+ +Y +   SKI       M  +L  T  GI ++YK
Sbjct: 591 LNRLLEVITTHVVSIEKYLDEQNSKIDRDYEEFMSEDLLSTLTGILDSYK 634

BLAST of Cp4.1LG14g04000 vs. Swiss-Prot
Match: NDC80_MACFA (Kinetochore protein NDC80 homolog OS=Macaca fascicularis GN=NDC80 PE=2 SV=1)

HSP 1 Score: 87.8 bits (216), Expect = 4.1e-16
Identity = 116/511 (22.70%), Postives = 220/511 (43.05%), Query Frame = 1

Query: 75  HSFNLTFKPFSPPSLKDLTETIHLL--LQCLDYH--PTKLEDDLPIVLKSLGYPFKINKG 134
           ++ N++ K    PS+KD  +    L    C  Y    TK E+++P + K LGYPF ++K 
Sbjct: 108 YAHNVSMKSLQAPSVKDFLKIFTFLYGFLCPSYELPDTKFEEEVPRIFKDLGYPFALSKS 167

Query: 135 ILKTPPAPHQWYPILALLHWLVQVAVYNYNLANDSDSF-------------VSSNDVNV- 194
            + T  APH W  I+A L WL+     +  +   S  F             +  N + + 
Sbjct: 168 SMYTVGAPHTWPHIVAALVWLIDCIKIHTAMKESSPLFDDGQPWGEETEDGIMHNKLFLD 227

Query: 195 YVLNSYVQYIRGDDDSVEALDQEFMERLKK-GRDDA--VESLNGLEMEVKELEAKAEALR 254
           Y +  Y  ++ G  DS + ++ E   +LK     DA  +ESL      + E  A+ E  R
Sbjct: 228 YTIKCYESFMSG-ADSFDEMNAELQSKLKDLFNVDAFKLESLEAKNRALNEQIARLEQER 287

Query: 255 -SDPSQREVLEKQQTVLEEDVKKFHTMIAGYSEKMQEMKKILEEKEKELEVKVQENKKIC 314
             +P++ E L K +  L+ DV+K+      Y   ++    IL++K   L  ++   +  C
Sbjct: 288 EKEPNRLESLRKLKASLQGDVQKYQ----AYMSNLESHSAILDQKLNGLNEEIARVELEC 347

Query: 315 E----ENEELKMRVESQMFNARDVERMRRELQAVERDIGDAEVSRNSWEEKTWDIDATVG 374
           E    EN  L+  +++Q ++  D+ER+  E   +++ I        + ++K W+ +    
Sbjct: 348 ETIKQENTRLQNIIDNQKYSVADIERINHERNELQQTINKLTKDLEAEQQKLWNEELKYA 407

Query: 375 NKFKELEAMARECNQAMRRLKLDNCHQYFLNAKGSTPADVMGVDYKTTIKPALDSFAEDI 434
              + +E    E ++  R+LK        L  KG+   +  G D++    P  ++ A  +
Sbjct: 408 RGKEAIETQLAEYHKLARKLK--------LIPKGA--ENSKGYDFEIKFNP--EAGANCL 467

Query: 435 KKSSMTKLEDLISLRQQSSENTAKIESRRSHIVSLQSHIEEVETQVNSLKNEIRDYTLRC 494
            K    + +  + L++  +E   +I   R+  + L+  +E++   +              
Sbjct: 468 VK---YRAQVYVPLKELLNETEEEINKARNKKMGLEDTLEQLNAMI-------------- 527

Query: 495 STESKKMVEDVQLEAHSIDVVESEAGEILKNAKLKHEEAIKQGEEEILICARELVACVDS 554
            TESK+ V  ++ E   +D +              +++ IK+ EEE   CA EL    +S
Sbjct: 528 -TESKRSVRTLKEEVQKLDDL--------------YQQKIKEAEEEDEKCASEL----ES 565

Query: 555 VSQYKEFVRSKISDMRSNLSKTAVGISEAYK 560
           + ++K  + S ++   S        +   Y+
Sbjct: 588 LEKHKHLLESTVNQGLSEAMNELDAVQREYQ 565

BLAST of Cp4.1LG14g04000 vs. Swiss-Prot
Match: NDC80_HUMAN (Kinetochore protein NDC80 homolog OS=Homo sapiens GN=NDC80 PE=1 SV=1)

HSP 1 Score: 85.9 bits (211), Expect = 1.6e-15
Identity = 111/524 (21.18%), Postives = 223/524 (42.56%), Query Frame = 1

Query: 75  HSFNLTFKPFSPPSLKDLTETIHLL--LQCLDYH--PTKLEDDLPIVLKSLGYPFKINKG 134
           ++ N++ K    PS+KD  +    L    C  Y    TK E+++P + K LGYPF ++K 
Sbjct: 108 YAHNVSMKSLQAPSVKDFLKIFTFLYGFLCPSYELPDTKFEEEVPRIFKDLGYPFALSKS 167

Query: 135 ILKTPPAPHQWYPILALLHWLVQVAVYNYNLANDSDSF-------------VSSNDVNV- 194
            + T  APH W  I+A L WL+     +  +   S  F             +  N + + 
Sbjct: 168 SMYTVGAPHTWPHIVAALVWLIDCIKIHTAMKESSPLFDDGQPWGEETEDGIMHNKLFLD 227

Query: 195 YVLNSYVQYIRGDDDSVEALDQEFMERLKK-GRDDA--VESLNGLEMEVKELEAKAEALR 254
           Y +  Y  ++ G  DS + ++ E   +LK     DA  +ESL      + E  A+ E  R
Sbjct: 228 YTIKCYESFMSG-ADSFDEMNAELQSKLKDLFNVDAFKLESLEAKNRALNEQIARLEQER 287

Query: 255 -SDPSQREVLEKQQTVLEEDVKKFHTMIAGYSEKMQEMKKILEEKEKELEVKVQENKKIC 314
             +P++ E L K +  L+ DV+K+      Y   ++    IL++K   L  ++   +  C
Sbjct: 288 EKEPNRLESLRKLKASLQGDVQKYQ----AYMSNLESHSAILDQKLNGLNEEIARVELEC 347

Query: 315 E----ENEELKMRVESQMFNARDVERMRRELQAVERDIGDAEVSRNSWEEKTWDIDATVG 374
           E    EN  L+  +++Q ++  D+ER+  E   +++ I        + ++K W+ +    
Sbjct: 348 ETIKQENTRLQNIIDNQKYSVADIERINHERNELQQTINKLTKDLEAEQQKLWNEELKYA 407

Query: 375 NKFKELEAMARECNQAMRRLKL-----DNCHQYFLNAKGSTPADV-MGVDYKTTIKPALD 434
              + +E    E ++  R+LKL     +N   Y    K +  A     V Y+  +   L 
Sbjct: 408 RGKEAIETQLAEYHKLARKLKLIPKGAENSKGYDFEIKFNPEAGANCLVKYRAQVYVPLK 467

Query: 435 SFAEDIKKSSMTKLEDLISLRQQSSENTAKIESRRSHIVSLQSHIEEVETQVNSLKNEIR 494
               + ++     L   + L     +  A I   +  + +L+  +++++        E  
Sbjct: 468 ELLNETEEEINKALNKKMGLEDTLEQLNAMITESKRSVRTLKEEVQKLDDLYQQKIKEAE 527

Query: 495 DYTLRCSTESKKMVEDVQLEAHSIDVVESEAGEILKNAKLKHEEAIKQGEEEILICAREL 554
           +   +C++E + + +   L   +++   SEA   L   + +++  ++   EE     R  
Sbjct: 528 EEDEKCASELESLEKHKHLLESTVNQGLSEAMNELDAVQREYQLVVQTTTEE-----RRK 587

Query: 555 VACVDSVSQYKEFVRSKISDMRSNLSKTAVGISEAYKNSLPAEL 567
           V   +++ +  E V + +  +  +L +    +   Y+  +  +L
Sbjct: 588 VG--NNLQRLLEMVATHVGSVEKHLEEQIAKVDREYEECMSEDL 619

BLAST of Cp4.1LG14g04000 vs. Swiss-Prot
Match: NDC80_XENTR (Kinetochore protein NDC80 homolog OS=Xenopus tropicalis GN=ndc80 PE=2 SV=1)

HSP 1 Score: 81.6 bits (200), Expect = 3.0e-14
Identity = 113/519 (21.77%), Postives = 230/519 (44.32%), Query Frame = 1

Query: 75  HSFNLTFKPFSPPSLKDLTETIHLLLQ--CLDYH--PTKLEDDLPIVLKSLGYPFKINKG 134
           +S  LT K    PS KD  +    +    C +Y    +K E+++P + K LGYPF ++K 
Sbjct: 107 YSQTLTVKSLQGPSTKDFLKIFAFIYTFICPNYENPESKFEEEIPRIFKELGYPFALSKS 166

Query: 135 ILKTPPAPHQWYPILALLHWLVQVAVYNYNLANDSDSF-------VSSNDVNV------Y 194
            + T  APH W  I+A L WL+        L +++  F        S N ++       Y
Sbjct: 167 SMYTVGAPHTWPQIVAALVWLIDCVKLCCVLRSENPVFEEPQMGEQSENGIDFNQLFLDY 226

Query: 195 VLNSYVQYIRGDDDSVEALDQEFMERLKKGRDDAVESLNGLEME----VKELEAKAEALR 254
            +  Y Q++ G  D+ E  D +   RLK+       +L  L+ E    ++E+E   +   
Sbjct: 227 TVRCYDQFMEG-RDTFEEYDADMCIRLKEAYHVDESNLEALQQESRRLLEEIERLEKEKE 286

Query: 255 SDPSQREVLEKQQTVLEEDVKKFHTMIAGYSEKMQEMKKILEEKEKELEVKVQENKKICE 314
           ++P +   + K +  L+ D++K+   +         +++ +    ++LE    E++ + +
Sbjct: 287 NEPDRLASMRKLKVSLQADIQKYQNYLTEIESHSTLLEQRVSSVSEDLEATELESRAVQQ 346

Query: 315 ENEELKMRVESQMFNARDVERMRRELQAVERDIGDAEVSRNSWEEKTWDIDATVGNKFKE 374
           EN  LK  +++Q ++  D+ER++ E   +++ I       +  +++ W  +       + 
Sbjct: 347 ENLRLKNILDNQKYSVADIERIKYEENELQQTIAKLTKDLDEDKQQLWSEELKYAKMKES 406

Query: 375 LEAMARECNQAMRRLKLDNCHQYFLNAKGSTPADVMGVDYKTTIKPALDSFAEDIKKSSM 434
           +E    E ++  R+++L      + N          G D++  I+  LDS     ++SS+
Sbjct: 407 VETQLSEFHKIGRKVRLIPPTAEYAN----------GYDFQ--IQCNLDS-----EQSSL 466

Query: 435 TKLEDLIS--LRQQSSENTAKIESRRSHIVSLQSHIEEVETQVNSLKNEI---RDYTLRC 494
               + I+  L +  S++  +I    +  + ++  +E++ T +   KNE+   +D   + 
Sbjct: 467 IHYRNKINVPLVEILSQSEGQIAGATNKKMGVEDMVEQLNTLIGEKKNEVKVQKDEAQKL 526

Query: 495 -STESKKMVEDVQLEAHSIDVVES--------EAG------EILKNAKLKHEE---AIKQ 550
             T  +K+ E  + E   I  +ES        E+G      E LK+ + + +E      Q
Sbjct: 527 EETYQQKVEEAEEDEKRWISEIESLEKHRQLLESGVNKSLDEALKDLQKEQQELQLVEHQ 586

BLAST of Cp4.1LG14g04000 vs. Swiss-Prot
Match: NDC80_XENLA (Kinetochore protein NDC80 homolog OS=Xenopus laevis GN=ndc80 PE=1 SV=2)

HSP 1 Score: 79.3 bits (194), Expect = 1.5e-13
Identity = 110/519 (21.19%), Postives = 224/519 (43.16%), Query Frame = 1

Query: 75  HSFNLTFKPFSPPSLKDLTETIHLLLQ--CLDYH--PTKLEDDLPIVLKSLGYPFKINKG 134
           +S  LT K    PS KD  +    +    C +Y    +K E+++P + K LGYPF ++K 
Sbjct: 107 YSQALTVKSLQGPSTKDFLKIFAFIYTFICPNYENPESKFEEEIPRIFKELGYPFALSKS 166

Query: 135 ILKTPPAPHQWYPILALLHWLVQVAVYNYNLANDSDSF-------VSSNDVNV------Y 194
            + T  APH W  I+A L WL+        L +++  F        S N ++       Y
Sbjct: 167 SMYTVGAPHTWPQIVAALVWLIDCVKLCCVLRSENPVFEDPPMGEQSENGIDFNQLFLDY 226

Query: 195 VLNSYVQYIRGDDDSVEALDQEFMERLKKGRDDAVESLNGLEMEVKELEAKAEAL----R 254
            +  Y Q++ G  D+ E  D +   RLK+       +L  L+ E + L  + E L     
Sbjct: 227 TVRCYDQFMEG-RDTFEEYDTDVCIRLKEAYHVDESNLEALQQESRRLMEEIERLEKEKE 286

Query: 255 SDPSQREVLEKQQTVLEEDVKKFHTMIAGYSEKMQEMKKILEEKEKELEVKVQENKKICE 314
           ++P +   + K +  L+ D++K+   +         + + +    +ELE    E++ I +
Sbjct: 287 NEPDRLASMRKLKASLQADIQKYQNYLTEIESHSTLLDQRVSSISEELEATELESRAIQQ 346

Query: 315 ENEELKMRVESQMFNARDVERMRRELQAVERDIGDAEVSRNSWEEKTWDIDATVGNKFKE 374
           EN  LK  +++Q ++  D+ER++ E   +++ I       +  +++ W  +       + 
Sbjct: 347 ENLRLKNILDNQKYSVADIERIKYEETELQQTIAKLTKDLDEDKQQLWHEELKYAKMKES 406

Query: 375 LEAMARECNQAMRRLKLDNCHQYFLNAKGSTPADVMGVDYKTTIKPALDSFAEDIKKSSM 434
           +E    E ++  R+++L      F N          G D++  I+  LDS     ++SS+
Sbjct: 407 VETQLSEFHKIGRKVRLIPPTAEFAN----------GYDFQ--IQCNLDS-----EQSSL 466

Query: 435 TKLEDLIS--LRQQSSENTAKIESRRSHIVSLQSHIEEVETQVNSLKNEIR--------- 494
               + I+  L +  S++   I S  +  + ++  +E++ T +   KNE++         
Sbjct: 467 MHYRNKINVPLVEILSQSEGHIASSTNKKMGVEDMVEQLNTLIGEKKNEVKVQKDEAQKL 526

Query: 495 ------------DYTLRCSTESKKMVEDVQLEAHSIDVVESEAGEILKNAKLKHEEAIKQ 550
                       ++  R  +E + + +  QL    ++    EA + L+  + + +    +
Sbjct: 527 EEIYQQKVEESEEHEKRWISEIESLEKHRQLLESGVNKSLDEAMKDLQKEQQELQLVEHR 586

BLAST of Cp4.1LG14g04000 vs. TrEMBL
Match: F6H969_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_12s0034g00950 PE=4 SV=1)

HSP 1 Score: 621.7 bits (1602), Expect = 8.9e-175
Identity = 339/573 (59.16%), Postives = 436/573 (76.09%), Query Frame = 1

Query: 1   MKGNGRRHPKGSFNPPPPPTP------QFRRDSDASFASSRPSSIGMARPAAASIDMYKE 60
           MK  GRR PK S  PPPPP P      QF RDSDASFASSRPSS+G+ R +A   D   +
Sbjct: 1   MKATGRRRPKDSIRPPPPPAPSVDHHRQFSRDSDASFASSRPSSLGINRSSAG--DALTD 60

Query: 61  RAVQISAVNTINSSLKELFNHSFNLTFKPFSPPSLKDLTETIHLLLQCLDYHPTKLEDDL 120
           R+ Q+SA+ TINS L    +HS  L+ KP   PS KD++ET+  L+  LD+  TKLEDDL
Sbjct: 61  RSYQLSAIRTINSFLA---SHSSPLSLKP-PLPSAKDISETLKFLISQLDFPTTKLEDDL 120

Query: 121 PIVLKSLGYPFKINKGILKTPPAPHQWYPILALLHWLVQVAVYNYNLANDSDSFVSSNDV 180
           PI+LK L  P K+NK  LK P  PH W P+L ++HWLVQ+A+YN +L ++   F   ++ 
Sbjct: 121 PILLKHLNCPIKLNKSALKAPGTPHAWPPLLGVMHWLVQIALYNDHLVSNLQCF---DNT 180

Query: 181 NVYVLNSYVQYIRGDDDSVEALDQEFMERLKKGRDDAVESLNGLEMEVKELEAKAEALRS 240
            VY L+SY+ YIRGDDD +EA+D+EFM +L+K RD   E +  LE EV E E + E LRS
Sbjct: 181 FVYALDSYLHYIRGDDDGMEAVDREFMGKLEKERDAVAEGVKALEKEVAEREGRLEELRS 240

Query: 241 DPSQREVLEKQQTVLEEDVKKFHTMIAGYSEKMQEMKKILEEKEKELEVKVQENKKICEE 300
            PS +EV+EK + VLEEDVKKFH +IA +S ++  ++KILEEKEKEL VKV+EN +ICEE
Sbjct: 241 GPSAKEVVEKVRGVLEEDVKKFHAIIAEFSGRIASVEKILEEKEKELGVKVEENNRICEE 300

Query: 301 NEELKMRVESQMFNARDVERMRRELQAVERDIGDAEVSRNSWEEKTWDIDATVGNKFKEL 360
           NEELK RVE Q FNARD ERM+RELQAVERDI +AEV+RN WEEK+WD+D T+G+KFKEL
Sbjct: 301 NEELKKRVELQTFNARDAERMKRELQAVERDITEAEVARNGWEEKSWDLDTTIGHKFKEL 360

Query: 361 EAMARECNQAMRRLKLDNCHQYFLNAKGSTPADVMGVDYKTTIKPALDSFAEDIKKSSMT 420
           EA++ ECNQA+RRLKL N  QY LNAKGS+P +V+G+DYK+ +KPALDSFA+DI KSSM+
Sbjct: 361 EALSIECNQALRRLKLGNGLQYVLNAKGSSPTEVLGIDYKSALKPALDSFADDINKSSMS 420

Query: 421 KLEDLISLRQQSSENTAKIESRRSHIVSLQSHIEEVETQVNSLKNEIRDYTLRCSTESKK 480
           KLE+LISL+QQS EN AKIE++R+ + +LQS  +EVE Q+N LK E ++YT RC+ E+KK
Sbjct: 421 KLEELISLQQQSVENAAKIEAKRNRLAALQSRSDEVEAQLNFLKKETQNYTSRCAMEAKK 480

Query: 481 MVEDVQLEAHSIDVVESEAGEILKNAKLKHEEAIKQGEEEILICARELVACVDSVSQYKE 540
           +VEDV++E H++D+VE E  ++L+ +KLK +EAIKQ EEEI ICA EL A VDSVS+YKE
Sbjct: 481 LVEDVEIETHNVDIVEREVADVLETSKLKLQEAIKQNEEEIQICAWELYALVDSVSKYKE 540

Query: 541 FVRSKISDMRSNLSKTAVGISEAYKNSLPAELG 568
            + SKIS+M+SNLS+TA  +S++YK SL A+ G
Sbjct: 541 HMASKISEMKSNLSETAGAVSDSYKGSLSAQFG 564

BLAST of Cp4.1LG14g04000 vs. TrEMBL
Match: F6I2P0_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_16s0013g00940 PE=4 SV=1)

HSP 1 Score: 613.6 bits (1581), Expect = 2.4e-172
Identity = 335/573 (58.46%), Postives = 436/573 (76.09%), Query Frame = 1

Query: 1   MKGNGRRHPKGSFNPPPPPTP------QFRRDSDASFASSRPSSIGMARPAAASIDMYKE 60
           MK  GRR PK SF PPPPP P      Q  RDSDASFA SRPSS+G++R +A   D   +
Sbjct: 215 MKATGRRRPKDSFRPPPPPPPSVDHHRQLSRDSDASFAGSRPSSLGISRSSAG--DALTD 274

Query: 61  RAVQISAVNTINSSLKELFNHSFNLTFKPFSPPSLKDLTETIHLLLQCLDYHPTKLEDDL 120
           R+ Q+SA+ TINS L    +HS  L+ KP   PS KD++ET+  L+  LD+  TKLEDDL
Sbjct: 275 RSYQLSAIRTINSFLA---SHSSPLSLKP-PLPSAKDISETLKFLISQLDFPTTKLEDDL 334

Query: 121 PIVLKSLGYPFKINKGILKTPPAPHQWYPILALLHWLVQVAVYNYNLANDSDSFVSSNDV 180
           PI+LK L  P K+NK  LK P  PH W  +L ++HWLVQ+A+YN +L ++   F   ++ 
Sbjct: 335 PILLKHLNCPIKLNKSALKAPGTPHAWPSLLGVMHWLVQIALYNDHLVSNLQCF---DNT 394

Query: 181 NVYVLNSYVQYIRGDDDSVEALDQEFMERLKKGRDDAVESLNGLEMEVKELEAKAEALRS 240
            VY L+SY+ YIRGDDD +EA+D+EFM +L++ RD   E +  LE EV E E + E LR 
Sbjct: 395 FVYALDSYLHYIRGDDDGMEAVDREFMGKLERERDAVAEGVKALEKEVAEREGRLEELRL 454

Query: 241 DPSQREVLEKQQTVLEEDVKKFHTMIAGYSEKMQEMKKILEEKEKELEVKVQENKKICEE 300
            PS +EV+EK++ VLEEDVKKFH +IA +S ++  ++KILEEKEKEL VKV+EN +ICEE
Sbjct: 455 GPSAKEVVEKERGVLEEDVKKFHAIIAEFSGRIASVEKILEEKEKELGVKVEENNRICEE 514

Query: 301 NEELKMRVESQMFNARDVERMRRELQAVERDIGDAEVSRNSWEEKTWDIDATVGNKFKEL 360
           NEELK RVE Q FNARD ERM+RELQAVERDI +AEV+RN WEEK+WD+D T+G+KFKEL
Sbjct: 515 NEELKKRVELQTFNARDAERMKRELQAVERDITEAEVARNGWEEKSWDLDTTIGHKFKEL 574

Query: 361 EAMARECNQAMRRLKLDNCHQYFLNAKGSTPADVMGVDYKTTIKPALDSFAEDIKKSSMT 420
           EA++ ECNQA+RRLKL N  QY LNAKGS+PA+V+G+DYK+T+KPALDSFA+DI KSSM+
Sbjct: 575 EALSIECNQALRRLKLGNGLQYVLNAKGSSPAEVLGIDYKSTLKPALDSFADDINKSSMS 634

Query: 421 KLEDLISLRQQSSENTAKIESRRSHIVSLQSHIEEVETQVNSLKNEIRDYTLRCSTESKK 480
           KLE+LISL+QQS EN AKIE++R+ + +LQS  +EVE Q+  LK E ++YT RC+TE+KK
Sbjct: 635 KLEELISLQQQSVENAAKIEAKRNRLAALQSSSDEVEAQLIFLKKESQNYTSRCATEAKK 694

Query: 481 MVEDVQLEAHSIDVVESEAGEILKNAKLKHEEAIKQGEEEILICARELVACVDSVSQYKE 540
           +VEDV++E H++D+VE E  ++L+ +KLK +EAIKQ EEEI +CA EL A VDSVS+YKE
Sbjct: 695 LVEDVEIETHNVDIVEREVADVLETSKLKLQEAIKQNEEEIQMCAWELYALVDSVSKYKE 754

Query: 541 FVRSKISDMRSNLSKTAVGISEAYKNSLPAELG 568
            + SKIS+M+ NLS+TA  +S++YK SL A+ G
Sbjct: 755 HMASKISEMKCNLSETAGAVSDSYKGSLLAQFG 778

BLAST of Cp4.1LG14g04000 vs. TrEMBL
Match: A5AI77_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_035303 PE=4 SV=1)

HSP 1 Score: 612.1 bits (1577), Expect = 7.0e-172
Identity = 336/573 (58.64%), Postives = 433/573 (75.57%), Query Frame = 1

Query: 1   MKGNGRRHPKGSFNPPPPPTP------QFRRDSDASFASSRPSSIGMARPAAASIDMYKE 60
           MK  GRR PK S  PPPPP P      QF RDSDASFASSRPSS+G+ R +A   D   +
Sbjct: 1   MKATGRRRPKDSIRPPPPPAPSVDHHRQFSRDSDASFASSRPSSLGINRSSAG--DALTD 60

Query: 61  RAVQISAVNTINSSLKELFNHSFNLTFKPFSPPSLKDLTETIHLLLQCLDYHPTKLEDDL 120
           R+ Q+SA+ TINS L    +HS  L+ KP   PS KD++ET+  L+  LD+  TKLEDDL
Sbjct: 61  RSYQLSAIRTINSFLA---SHSSPLSLKP-PLPSAKDISETLKFLISQLDFPTTKLEDDL 120

Query: 121 PIVLKSLGYPFKINKGILKTPPAPHQWYPILALLHWLVQVAVYNYNLANDSDSFVSSNDV 180
           PI+LK L  P K+NK  LK P  PH W P+L ++HWLVQ+A+YN +L ++   F   ++ 
Sbjct: 121 PILLKHLNCPIKLNKSALKAPGTPHAWPPLLGVMHWLVQIALYNDHLVSNLQCF---DNT 180

Query: 181 NVYVLNSYVQYIRGDDDSVEALDQEFMERLKKGRDDAVESLNGLEMEVKELEAKAEALRS 240
            VY L+SY+ YIRGDDD +EA+D+EFM +L+  RD   E +  LE EV E E + E LRS
Sbjct: 181 FVYALDSYLHYIRGDDDGMEAVDREFMGKLEXERDAVAEGVKALEKEVAEREGRLEELRS 240

Query: 241 DPSQREVLEKQQTVLEEDVKKFHTMIAGYSEKMQEMKKILEEKEKELEVKVQENKKICEE 300
            PS +EV+EK++ VLEEDVKKFH +IA +S ++  ++KILEEKEKEL VKV+EN +ICEE
Sbjct: 241 GPSAKEVVEKERGVLEEDVKKFHAIIAEFSGRIASVEKILEEKEKELGVKVEENNRICEE 300

Query: 301 NEELKMRVESQMFNARDVERMRRELQAVERDIGDAEVSRNSWEEKTWDIDATVGNKFKEL 360
           NEELK RVE Q FNARD ERM+RELQAVERDI +AEV+RN WEEK+WD+D T+G+KFKEL
Sbjct: 301 NEELKKRVELQTFNARDAERMKRELQAVERDITEAEVARNGWEEKSWDLDTTIGHKFKEL 360

Query: 361 EAMARECNQAMRRLKLDNCHQYFLNAKGSTPADVMGVDYKTTIKPALDSFAEDIKKSSMT 420
           EA++ ECNQA+RRLKL N  QY LNAKGS+P +V+G+DYK+ +KPALDSFA+DI KSSM+
Sbjct: 361 EALSIECNQALRRLKLGNGLQYVLNAKGSSPXEVLGIDYKSXLKPALDSFADDINKSSMS 420

Query: 421 KLEDLISLRQQSSENTAKIESRRSHIVSLQSHIEEVETQVNSLKNEIRDYTLRCSTESKK 480
           KLE+LISL+QQS EN AKIE++R+ + +LQS  +EVE Q+  LK E ++YT RC+TE+KK
Sbjct: 421 KLEELISLQQQSVENAAKIEAKRNRLAALQSXSDEVEAQLIFLKKESQNYTSRCATEAKK 480

Query: 481 MVEDVQLEAHSIDVVESEAGEILKNAKLKHEEAIKQGEEEILICARELVACVDSVSQYKE 540
           +VEDV++E H++D VE E  ++L+ +KLK +EAIKQ EEEI  CA EL A VDSVS+YKE
Sbjct: 481 LVEDVEIETHNVDXVEREVADVLETSKLKLQEAIKQNEEEIQXCAWELYALVDSVSKYKE 540

Query: 541 FVRSKISDMRSNLSKTAVGISEAYKNSLPAELG 568
            + SKIS+M+ NLS+TA  +S++YK SL A+ G
Sbjct: 541 HMASKISEMKCNLSETAGAVSDSYKGSLLAQFG 564

BLAST of Cp4.1LG14g04000 vs. TrEMBL
Match: M5XWK4_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa003403mg PE=4 SV=1)

HSP 1 Score: 590.1 bits (1520), Expect = 2.9e-165
Identity = 324/569 (56.94%), Postives = 436/569 (76.63%), Query Frame = 1

Query: 7   RHPKGSFNP-PPPPTP--QFR-RDSDASFASSRPSSIGMARPAAASIDMYKERAVQISAV 66
           R PK SFNP PPP TP   FR RDSDASFASSRPSS+GM  P + ++D+YK+R+ Q + +
Sbjct: 9   RRPKESFNPRPPPATPLDHFRHRDSDASFASSRPSSVGMG-PTSNALDLYKDRSFQQATL 68

Query: 67  NTINSSLKELFNHSFNLTFKPFSPPSLKDLTETIHLLLQCLDYHPTKLEDDLPIVLKSLG 126
           ++INS L    +HS  + F  F  PS K++TET+  L+  LDY   KLEDDLP++LK L 
Sbjct: 69  SSINSYLA---SHSLQI-FLKFPIPSAKEITETLKFLVARLDYPNPKLEDDLPVILKFLK 128

Query: 127 YPFKINKGILKTPPA-PHQWYPILALLHWLVQVAVYNYNLANDSDSFVSSNDVNVYVLNS 186
            PFK NK +L+ P    HQW  +LA++HWL Q+ +YN +LA++S +FV +N ++ Y L S
Sbjct: 129 CPFKPNKSVLRNPTVNHHQWPTLLAVIHWLFQIVLYNDHLASNSGAFVDNNAMSAYALES 188

Query: 187 YVQYIRGDDDSVEALDQEFMERLKKGRDDAVESLNGLEMEVKELEAKAEALRSDPSQREV 246
           Y  YI GDDDSVEALD++F+E+L+  R++A ES+  LE    ELE K E +RS PS+RE 
Sbjct: 189 YSYYISGDDDSVEALDRQFLEKLEAERENAEESIRVLEATAAELEGKMEEMRSGPSKREA 248

Query: 247 LEKQQTVLEEDVKKFHTMIAGYSEKMQEMKKILEEKEKELEVKVQENKKICEENEELKMR 306
           LEK++ VLE+DV KF+ MI   ++ + +++ +LE KEKELE KV + ++ICEENEELK R
Sbjct: 249 LEKERGVLEQDVNKFNEMIGSLAKSVAKLEAVLEAKEKELEAKVADTRRICEENEELKKR 308

Query: 307 VESQMFNARDVERMRRELQAVERDIGDAEVSRNSWEEKTWDIDATVGNKFKELEAMAREC 366
           VE Q FNARDV+RM+RELQAVERDIG+AE++RN+WEEK WD+D  + +KFKELE +A EC
Sbjct: 309 VELQTFNARDVDRMKRELQAVERDIGEAELARNAWEEKAWDLDTMLSHKFKELETLAMEC 368

Query: 367 NQAMRRLKLDNCHQYFLNAKGSTPADVMGVDYKTTIKPALDSFAEDIKKSSMTKLEDLIS 426
           NQAMRRLKL N  QY LNAKGST ADVMG+DYK T+KPAL+S+++DIKKSS+ KLE+LIS
Sbjct: 369 NQAMRRLKLGNGFQYVLNAKGSTAADVMGIDYKLTLKPALNSYSDDIKKSSVEKLEELIS 428

Query: 427 LRQQSSENTAKIESRRSHIVSLQSHIEEVETQVNSLKNEIRDYTLRCSTESKKMVEDVQL 486
           L+QQSSE +A IE +R+ I +LQSHI+EVE Q+N LK E  DYT RC+ E+++M++DVQ+
Sbjct: 429 LQQQSSELSATIEGKRNFIATLQSHIDEVEAQLNLLKKETHDYTYRCAIEARQMMDDVQM 488

Query: 487 EAHSIDVVESEAGEILKNAKLKHEEAIKQGEEEILICARELVACVDSVSQYKEFVRSKIS 546
           EAH++D++E +A EILK +KLK +E IKQ EEE   CA EL+  +DSVS++KE+V+SKI 
Sbjct: 489 EAHNLDILERDAEEILKTSKLKLQETIKQTEEETKKCAYELMTVIDSVSKHKEYVQSKIL 548

Query: 547 DMRSNLSKTAVGISEAYKNSLPAELGTVS 571
           +MR ++S+TAV +S+A+K SL ++ G +S
Sbjct: 549 EMRRDVSETAVAVSDAHKGSLQSQFGFLS 572

BLAST of Cp4.1LG14g04000 vs. TrEMBL
Match: A0A067LHE2_JATCU (Uncharacterized protein OS=Jatropha curcas GN=JCGZ_16911 PE=4 SV=1)

HSP 1 Score: 580.1 bits (1494), Expect = 3.0e-162
Identity = 320/575 (55.65%), Postives = 422/575 (73.39%), Query Frame = 1

Query: 1   MKGNGRRHPKGSFNPPPPPTPQ----------FRRDSDASFASSRPSSIGMARPAAASID 60
           M+GN RR PK SF P P P  +            RDSDASFASSRPS++G+ R +    +
Sbjct: 1   MRGNSRRQPKDSFIPQPTPDRRQYPFSGAAGNTSRDSDASFASSRPSTVGVGRTS----E 60

Query: 61  MYKERAVQISAVNTINSSLKELFNHSFNLTFKPFSPPSLKDLTETIHLLLQCLDYHPTKL 120
           +Y +R  Q SAV +IN+ L    +H+  L+ +     S K++TE +  LL  LDY   KL
Sbjct: 61  LYTDRGHQNSAVRSINTFLS---SHNSLLSLRTHPISSAKEMTEVLQFLLHQLDYPKAKL 120

Query: 121 EDDLPIVLKSLGYPFKINKGILKTPPAPHQWYPILALLHWLVQVAVYNYNLANDSDSFVS 180
           +DDL ++LKSL  PFK+NK  L+ P  PH W   LAL+HWLVQ+A+Y  +LA +S S V 
Sbjct: 121 DDDLFVILKSLNCPFKVNKSALRAPNTPHNWPSFLALIHWLVQIAMYAEHLAANSRSLVE 180

Query: 181 SNDVNVYVLNSYVQYIRGDDDSVEALDQEFMERLKKGRDDAVESLNGLEMEVKELEAKAE 240
           +N ++VY L+SY+ +I G D++VE LD+EFME+L++ RD  +ES+  LE    EL  KAE
Sbjct: 181 TNGMHVYTLDSYLNFINGHDEAVEELDREFMEKLERERDSVLESVKNLEANFNELSTKAE 240

Query: 241 ALRSDPSQREVLEKQQTVLEEDVKKFHTMIAGYSEKMQEMKKILEEKEKELEVKVQENKK 300
            LR+ P++RE LEKQ++VLEEDV KF+ MIA ++++M+ M+ ++EEK KELE KV+E K+
Sbjct: 241 ELRTGPTEREKLEKQRSVLEEDVNKFNVMIADFNQRMKAMENLVEEKRKELEAKVEEKKR 300

Query: 301 ICEENEELKMRVESQMFNARDVERMRRELQAVERDIGDAEVSRNSWEEKTWDIDATVGNK 360
           I EENEELK RV+ Q FNARD ERM+RELQAVE  IG+AE SRNSWEEK WD+DA +G++
Sbjct: 301 IDEENEELKKRVDDQSFNARDAERMKRELQAVENGIGEAEASRNSWEEKIWDLDAAIGHE 360

Query: 361 FKELEAMARECNQAMRRLKLDNCHQYFLNAKGSTPADVMGVDYKTTIKPALDSFAEDIKK 420
            KELE++A ECNQA++RLKL N  QY LNAKGSTPA VMGVDYK+T+KP L+SFA+DIKK
Sbjct: 361 LKELESLAMECNQAVKRLKLGNGFQYVLNAKGSTPAQVMGVDYKSTVKPGLESFADDIKK 420

Query: 421 SSMTKLEDLISLRQQSSENTAKIESRRSHIVSLQSHIEEVETQVNSLKNEIRDYTLRCST 480
           SSM KLE+LISL+Q+ SE TAK E +++    LQ HI+EVET +N L+ E  +YT RC+ 
Sbjct: 421 SSMEKLEELISLQQELSELTAKTEGKKNRTAVLQCHIDEVETLLNLLRKETEEYTNRCAM 480

Query: 481 ESKKMVEDVQLEAHSIDVVESEAGEILKNAKLKHEEAIKQGEEEILICARELVACVDSVS 540
           E+KK++EDVQ+EAH++DV+E EA  ILK  +LK +EAIKQ EEEI + A +L A +D+VS
Sbjct: 481 EAKKLIEDVQMEAHNLDVLEREAAAILKANELKLQEAIKQSEEEIQMHAIDLFAVIDTVS 540

Query: 541 QYKEFVRSKISDMRSNLSKTAVGISEAYKNSLPAE 566
           +YKE V SKIS+MRS LS+T   IS+AYK+SLP E
Sbjct: 541 EYKEHVESKISEMRSKLSETVGAISDAYKHSLPVE 568

BLAST of Cp4.1LG14g04000 vs. TAIR10
Match: AT3G54630.1 (AT3G54630.1 Kinetochore protein Ndc80 (InterPro:IPR005550))

HSP 1 Score: 428.3 bits (1100), Expect = 7.3e-120
Identity = 245/572 (42.83%), Postives = 372/572 (65.03%), Query Frame = 1

Query: 6   RRHPKGSFNPPPPPTPQFR--------RDSDASFASSRPSSIGMARPAAASIDMYKERAV 65
           RR   G    PPPP P           RDSDASFASSRPSSIG+    A+      +R+ 
Sbjct: 9   RRTTVGFGGAPPPPPPSIEQQRHLFNSRDSDASFASSRPSSIGLGGRGASD-----DRSS 68

Query: 66  QISAVNTINSSLKELFNHSFNLTFKPFSPPSLKDLTETIHLLLQCLDY--HPTKLEDDLP 125
            I  +N   S+      H+F ++ +    PS+KD++ET+  LL  LDY     K ++DL 
Sbjct: 69  MIRFINAFLST------HNFPISIRGNPVPSVKDISETLKFLLSALDYPCDSIKWDEDLV 128

Query: 126 IVLKSLGYPFKINKGILKTPPAPHQWYPILALLHWLVQVAVYNYNLANDSDSFVSSNDVN 185
             LKS   PFKI K  LK P  PH W  +LA++HWL ++A ++ +L ++S S    N +N
Sbjct: 129 FFLKSQKCPFKITKSSLKAPNTPHNWPTVLAVVHWLAELARFHQHLVSNSTSVPEDNSMN 188

Query: 186 VYVLNSYVQYIRGDDDSVEALDQEFMERLKKGRDDAVESLNGLEMEVKELEAKAEALRSD 245
            + + S+  +IRG+DD V  LD +F+ +L+  +    E+++G E    ELEAK E+LR  
Sbjct: 189 FFAIQSFGHFIRGEDDKVNDLDSQFLGKLEAEKTSVAETISGCEKISGELEAKLESLRKG 248

Query: 246 PSQREVLEKQQTVLEEDVKKFHTMIAGYSEKMQEMKKILEEKEKELEVKVQENKKICEEN 305
           PS++E LEK +  LE DV KF T++  Y+++   M+K++EEK KEL+ K +E ++I  EN
Sbjct: 249 PSKKESLEKVKADLENDVNKFRTIVVEYTDRNPAMEKVVEEKAKELKAKEEERERISVEN 308

Query: 306 EELKMRVESQMFNARDVERMRRELQAVERDIGDAEVSRNSWEEKTWDIDATVGNKFKELE 365
           +ELK  VE Q F+A DV RMRRELQAVERD+ DAEV+R+ W++K W++++ + N+F +++
Sbjct: 309 KELKKSVELQNFSAADVNRMRRELQAVERDVADAEVARDGWDQKAWELNSQIRNQFHQIQ 368

Query: 366 AMARECNQAMRRLKLDNCHQYFLNAKGSTPADVMGVDYKTTIKPALDSFAEDIKKSSMTK 425
            +A +CNQA+RRLKLD   Q+ +N +G TPA VMGVDYK+ +KPAL S  + IK SS  K
Sbjct: 369 TLAIDCNQALRRLKLD--IQFAVNERGETPAAVMGVDYKSVVKPALCSLCDGIKGSSAEK 428

Query: 426 LEDLISLRQQSSENTAKIESRRSHIVSLQSHIEEVETQVNSLKNEIRDYTLRCSTESKKM 485
           +E+L++L+   SE  +KIES+RS + S+Q  I ++E ++  +K E ++ + +C  E+K +
Sbjct: 429 VEELVTLQHHKSEMASKIESKRSLLGSIQLQINDLEEKMKLVKKETQELSTKCDLEAKTL 488

Query: 486 VEDVQLEAHSIDVVESEAGEILKNAKLKHEEAIKQGEEEILICARELVACVDSVSQYKEF 545
           VE V+ EA +++VVE EA E +K ++L+ +EA+K+ EEE+  CA +L A +DS+S+ KE+
Sbjct: 489 VESVKAEALNLEVVEKEAAEFVKASELRLQEAVKESEEEVQACAAQLFALIDSISKQKEY 548

Query: 546 VRSKISDMRSNLSKTAVGISEAYKNSLPAELG 568
           + SKIS++++ ++ TA  +SE YK +    LG
Sbjct: 549 MDSKISEIKTGVADTASAVSEIYKANFKKNLG 567

BLAST of Cp4.1LG14g04000 vs. NCBI nr
Match: gi|778680142|ref|XP_004149971.2| (PREDICTED: kinetochore protein NDC80 homolog [Cucumis sativus])

HSP 1 Score: 901.4 bits (2328), Expect = 8.3e-259
Identity = 469/565 (83.01%), Postives = 516/565 (91.33%), Query Frame = 1

Query: 1   MKGNGRRHPKGSFNPPPPPTPQFRRDSDASFASSRPSSIGMARPAAASIDMYKERAVQIS 60
           MKG  RR PK SFNPPPPPTPQFRRDSD SFASSRPSSIGM+RP++ASIDMYKERAVQIS
Sbjct: 1   MKGTARRRPKASFNPPPPPTPQFRRDSDVSFASSRPSSIGMSRPSSASIDMYKERAVQIS 60

Query: 61  AVNTINSSLKELFNHSFNLTFKPFSPPSLKDLTETIHLLLQCLDYHPTKLEDDLPIVLKS 120
            VNTINSSLKELF+HSFN+TFKPFSPPSLKDLTETI+LLLQCLDY PTKLEDDLPI+LKS
Sbjct: 61  TVNTINSSLKELFSHSFNVTFKPFSPPSLKDLTETINLLLQCLDYPPTKLEDDLPILLKS 120

Query: 121 LGYPFKINKGILKTPPAPHQWYPILALLHWLVQVAVYNYNLANDSDSFVSSNDVNVYVLN 180
           LGYPFKINK ILK PPAPHQWY ILALLHWLVQVA+YNYNLANDS+   + +DVN+YVLN
Sbjct: 121 LGYPFKINKNILKNPPAPHQWYQILALLHWLVQVALYNYNLANDSNPIFTGHDVNIYVLN 180

Query: 181 SYVQYIRGDDDSVEALDQEFMERLKKGRDDAVESLNGLEMEVKELEAKAEALRSDPSQRE 240
           SY+ Y+RGDDDSVEALDQEFMERLKK RDD+ ESL  LE EVK LEAK EALRSDPSQRE
Sbjct: 181 SYLHYMRGDDDSVEALDQEFMERLKKTRDDSRESLKELEEEVKVLEAKVEALRSDPSQRE 240

Query: 241 VLEKQQTVLEEDVKKFHTMIAGYSEKMQEMKKILEEKEKELEVKVQENKKICEENEELKM 300
           VLEKQQ++LEEDVKKFHTMIAGYSEKMQEMKK+LEEKEKEL+VK++E  KICEENEELK 
Sbjct: 241 VLEKQQSLLEEDVKKFHTMIAGYSEKMQEMKKLLEEKEKELDVKMEERNKICEENEELKK 300

Query: 301 RVESQMFNARDVERMRRELQAVERDIGDAEVSRNSWEEKTWDIDATVGNKFKELEAMARE 360
           RVESQ+FN RDVERMRRELQAVERDIGDAEVSRNSWEEK+WD+DAT+GNKFKELEA+A E
Sbjct: 301 RVESQLFNPRDVERMRRELQAVERDIGDAEVSRNSWEEKSWDLDATLGNKFKELEAIAME 360

Query: 361 CNQAMRRLKLDNCHQYFLNAKGSTPADVMGVDYKTTIKPALDSFAEDIKKSSMTKLEDLI 420
           CNQAMRRLKLD+CHQY LNAKGSTPADVMG+DYKTT+KPALDSFAEDI++SSM KLE+LI
Sbjct: 361 CNQAMRRLKLDSCHQYVLNAKGSTPADVMGIDYKTTLKPALDSFAEDIRRSSMMKLEELI 420

Query: 421 SLRQQSSENTAKIESRRSHIVSLQSHIEEVETQVNSLKNEIRDYTLRCSTESKKMVEDVQ 480
           SLRQQSSENTAKIES+R HI SLQSH+ E ETQ+ SLK+E+RDY LRCSTESKKMVEDVQ
Sbjct: 421 SLRQQSSENTAKIESKRDHIASLQSHVNEHETQLISLKDEMRDYILRCSTESKKMVEDVQ 480

Query: 481 LEAHSIDVVESEAGEILKNAKLKHEEAIKQGEEEILICARELVACVDSVSQYKEFVRSKI 540
            EAH++D+VE EA E LKNAKL H+EAIKQGEEEI +CAREL++C+DSVSQYK  V  KI
Sbjct: 481 QEAHNLDLVEGEAEESLKNAKLAHQEAIKQGEEEIHMCARELLSCLDSVSQYKAVVSCKI 540

Query: 541 SDMRSNLSKTAVGISEAYKNSLPAE 566
           SDM S LSK A GISEA+KNS+PAE
Sbjct: 541 SDMESCLSKAAAGISEAFKNSMPAE 565

BLAST of Cp4.1LG14g04000 vs. NCBI nr
Match: gi|659077518|ref|XP_008439248.1| (PREDICTED: kinetochore protein NDC80 homolog [Cucumis melo])

HSP 1 Score: 888.3 bits (2294), Expect = 7.3e-255
Identity = 464/565 (82.12%), Postives = 512/565 (90.62%), Query Frame = 1

Query: 1   MKGNGRRHPKGSFNPPPPPTPQFRRDSDASFASSRPSSIGMARPAAASIDMYKERAVQIS 60
           MKG GRR PK SFNPPPPPTPQFRRDSD SFASSRPSSIGM+RP +ASIDMYKERAVQIS
Sbjct: 1   MKGTGRRRPKASFNPPPPPTPQFRRDSDVSFASSRPSSIGMSRPPSASIDMYKERAVQIS 60

Query: 61  AVNTINSSLKELFNHSFNLTFKPFSPPSLKDLTETIHLLLQCLDYHPTKLEDDLPIVLKS 120
            VNTINSSLKELF+HSFN+TFKPFSPPSLKDLTETI+LLLQCLDY PTKLEDDLPI+LKS
Sbjct: 61  TVNTINSSLKELFSHSFNVTFKPFSPPSLKDLTETINLLLQCLDYPPTKLEDDLPILLKS 120

Query: 121 LGYPFKINKGILKTPPAPHQWYPILALLHWLVQVAVYNYNLANDSDSFVSSNDVNVYVLN 180
           LGYPFKINK ILKTPPAPHQWY ILALLHWLVQVA+YNYNLANDS+     +DVN+YVLN
Sbjct: 121 LGYPFKINKNILKTPPAPHQWYQILALLHWLVQVALYNYNLANDSNPVFMGHDVNIYVLN 180

Query: 181 SYVQYIRGDDDSVEALDQEFMERLKKGRDDAVESLNGLEMEVKELEAKAEALRSDPSQRE 240
           SY+ Y+RGDDDSVEALDQEFMERLKK RDD+ ESL  LE EVK LEAK EALRSDPSQRE
Sbjct: 181 SYLHYMRGDDDSVEALDQEFMERLKKTRDDSCESLKELEEEVKVLEAKVEALRSDPSQRE 240

Query: 241 VLEKQQTVLEEDVKKFHTMIAGYSEKMQEMKKILEEKEKELEVKVQENKKICEENEELKM 300
           VLEKQQ++LEEDVKKFHTMIAGYSEKMQEMKK+LEEKEKELEVK++E  KICEENEELK 
Sbjct: 241 VLEKQQSLLEEDVKKFHTMIAGYSEKMQEMKKLLEEKEKELEVKMEERNKICEENEELKK 300

Query: 301 RVESQMFNARDVERMRRELQAVERDIGDAEVSRNSWEEKTWDIDATVGNKFKELEAMARE 360
           RVESQ+FN RDVERMRRELQAVERDIGDAEVSRNSWEEK+WD+DAT+GNKFKELEA+A E
Sbjct: 301 RVESQLFNPRDVERMRRELQAVERDIGDAEVSRNSWEEKSWDLDATLGNKFKELEAIAME 360

Query: 361 CNQAMRRLKLDNCHQYFLNAKGSTPADVMGVDYKTTIKPALDSFAEDIKKSSMTKLEDLI 420
           CNQAMRRLKLD+CHQY LNAKGSTPADVMG+DYK+T+KPALDSFAEDI++SSM KLE+LI
Sbjct: 361 CNQAMRRLKLDSCHQYVLNAKGSTPADVMGIDYKSTVKPALDSFAEDIRRSSMMKLEELI 420

Query: 421 SLRQQSSENTAKIESRRSHIVSLQSHIEEVETQVNSLKNEIRDYTLRCSTESKKMVEDVQ 480
           SLRQQSSENTAKI+S+R+ I SLQSHI E ET++ SLK+E+RDY LRCSTESKKMVED Q
Sbjct: 421 SLRQQSSENTAKIDSKRNQIASLQSHINEHETKLISLKDEMRDYILRCSTESKKMVEDFQ 480

Query: 481 LEAHSIDVVESEAGEILKNAKLKHEEAIKQGEEEILICARELVACVDSVSQYKEFVRSKI 540
            EAH++D+VE EA E LKNAKL ++EA KQ EEEI +CAREL++C+DSVSQYK  V  KI
Sbjct: 481 QEAHNLDLVEGEAEESLKNAKLANQEATKQEEEEIHMCARELLSCLDSVSQYKAVVSCKI 540

Query: 541 SDMRSNLSKTAVGISEAYKNSLPAE 566
           SDM S LSK A GISEA+KNS+P E
Sbjct: 541 SDMESCLSKAAAGISEAFKNSMPVE 565

BLAST of Cp4.1LG14g04000 vs. NCBI nr
Match: gi|225448101|ref|XP_002273942.1| (PREDICTED: kinetochore protein ndc80-like [Vitis vinifera])

HSP 1 Score: 621.7 bits (1602), Expect = 1.3e-174
Identity = 339/573 (59.16%), Postives = 436/573 (76.09%), Query Frame = 1

Query: 1   MKGNGRRHPKGSFNPPPPPTP------QFRRDSDASFASSRPSSIGMARPAAASIDMYKE 60
           MK  GRR PK S  PPPPP P      QF RDSDASFASSRPSS+G+ R +A   D   +
Sbjct: 1   MKATGRRRPKDSIRPPPPPAPSVDHHRQFSRDSDASFASSRPSSLGINRSSAG--DALTD 60

Query: 61  RAVQISAVNTINSSLKELFNHSFNLTFKPFSPPSLKDLTETIHLLLQCLDYHPTKLEDDL 120
           R+ Q+SA+ TINS L    +HS  L+ KP   PS KD++ET+  L+  LD+  TKLEDDL
Sbjct: 61  RSYQLSAIRTINSFLA---SHSSPLSLKP-PLPSAKDISETLKFLISQLDFPTTKLEDDL 120

Query: 121 PIVLKSLGYPFKINKGILKTPPAPHQWYPILALLHWLVQVAVYNYNLANDSDSFVSSNDV 180
           PI+LK L  P K+NK  LK P  PH W P+L ++HWLVQ+A+YN +L ++   F   ++ 
Sbjct: 121 PILLKHLNCPIKLNKSALKAPGTPHAWPPLLGVMHWLVQIALYNDHLVSNLQCF---DNT 180

Query: 181 NVYVLNSYVQYIRGDDDSVEALDQEFMERLKKGRDDAVESLNGLEMEVKELEAKAEALRS 240
            VY L+SY+ YIRGDDD +EA+D+EFM +L+K RD   E +  LE EV E E + E LRS
Sbjct: 181 FVYALDSYLHYIRGDDDGMEAVDREFMGKLEKERDAVAEGVKALEKEVAEREGRLEELRS 240

Query: 241 DPSQREVLEKQQTVLEEDVKKFHTMIAGYSEKMQEMKKILEEKEKELEVKVQENKKICEE 300
            PS +EV+EK + VLEEDVKKFH +IA +S ++  ++KILEEKEKEL VKV+EN +ICEE
Sbjct: 241 GPSAKEVVEKVRGVLEEDVKKFHAIIAEFSGRIASVEKILEEKEKELGVKVEENNRICEE 300

Query: 301 NEELKMRVESQMFNARDVERMRRELQAVERDIGDAEVSRNSWEEKTWDIDATVGNKFKEL 360
           NEELK RVE Q FNARD ERM+RELQAVERDI +AEV+RN WEEK+WD+D T+G+KFKEL
Sbjct: 301 NEELKKRVELQTFNARDAERMKRELQAVERDITEAEVARNGWEEKSWDLDTTIGHKFKEL 360

Query: 361 EAMARECNQAMRRLKLDNCHQYFLNAKGSTPADVMGVDYKTTIKPALDSFAEDIKKSSMT 420
           EA++ ECNQA+RRLKL N  QY LNAKGS+P +V+G+DYK+ +KPALDSFA+DI KSSM+
Sbjct: 361 EALSIECNQALRRLKLGNGLQYVLNAKGSSPTEVLGIDYKSALKPALDSFADDINKSSMS 420

Query: 421 KLEDLISLRQQSSENTAKIESRRSHIVSLQSHIEEVETQVNSLKNEIRDYTLRCSTESKK 480
           KLE+LISL+QQS EN AKIE++R+ + +LQS  +EVE Q+N LK E ++YT RC+ E+KK
Sbjct: 421 KLEELISLQQQSVENAAKIEAKRNRLAALQSRSDEVEAQLNFLKKETQNYTSRCAMEAKK 480

Query: 481 MVEDVQLEAHSIDVVESEAGEILKNAKLKHEEAIKQGEEEILICARELVACVDSVSQYKE 540
           +VEDV++E H++D+VE E  ++L+ +KLK +EAIKQ EEEI ICA EL A VDSVS+YKE
Sbjct: 481 LVEDVEIETHNVDIVEREVADVLETSKLKLQEAIKQNEEEIQICAWELYALVDSVSKYKE 540

Query: 541 FVRSKISDMRSNLSKTAVGISEAYKNSLPAELG 568
            + SKIS+M+SNLS+TA  +S++YK SL A+ G
Sbjct: 541 HMASKISEMKSNLSETAGAVSDSYKGSLSAQFG 564

BLAST of Cp4.1LG14g04000 vs. NCBI nr
Match: gi|225455018|ref|XP_002279662.1| (PREDICTED: kinetochore protein ndc80 [Vitis vinifera])

HSP 1 Score: 613.6 bits (1581), Expect = 3.5e-172
Identity = 335/573 (58.46%), Postives = 436/573 (76.09%), Query Frame = 1

Query: 1   MKGNGRRHPKGSFNPPPPPTP------QFRRDSDASFASSRPSSIGMARPAAASIDMYKE 60
           MK  GRR PK SF PPPPP P      Q  RDSDASFA SRPSS+G++R +A   D   +
Sbjct: 1   MKATGRRRPKDSFRPPPPPPPSVDHHRQLSRDSDASFAGSRPSSLGISRSSAG--DALTD 60

Query: 61  RAVQISAVNTINSSLKELFNHSFNLTFKPFSPPSLKDLTETIHLLLQCLDYHPTKLEDDL 120
           R+ Q+SA+ TINS L    +HS  L+ KP   PS KD++ET+  L+  LD+  TKLEDDL
Sbjct: 61  RSYQLSAIRTINSFLA---SHSSPLSLKP-PLPSAKDISETLKFLISQLDFPTTKLEDDL 120

Query: 121 PIVLKSLGYPFKINKGILKTPPAPHQWYPILALLHWLVQVAVYNYNLANDSDSFVSSNDV 180
           PI+LK L  P K+NK  LK P  PH W  +L ++HWLVQ+A+YN +L ++   F   ++ 
Sbjct: 121 PILLKHLNCPIKLNKSALKAPGTPHAWPSLLGVMHWLVQIALYNDHLVSNLQCF---DNT 180

Query: 181 NVYVLNSYVQYIRGDDDSVEALDQEFMERLKKGRDDAVESLNGLEMEVKELEAKAEALRS 240
            VY L+SY+ YIRGDDD +EA+D+EFM +L++ RD   E +  LE EV E E + E LR 
Sbjct: 181 FVYALDSYLHYIRGDDDGMEAVDREFMGKLERERDAVAEGVKALEKEVAEREGRLEELRL 240

Query: 241 DPSQREVLEKQQTVLEEDVKKFHTMIAGYSEKMQEMKKILEEKEKELEVKVQENKKICEE 300
            PS +EV+EK++ VLEEDVKKFH +IA +S ++  ++KILEEKEKEL VKV+EN +ICEE
Sbjct: 241 GPSAKEVVEKERGVLEEDVKKFHAIIAEFSGRIASVEKILEEKEKELGVKVEENNRICEE 300

Query: 301 NEELKMRVESQMFNARDVERMRRELQAVERDIGDAEVSRNSWEEKTWDIDATVGNKFKEL 360
           NEELK RVE Q FNARD ERM+RELQAVERDI +AEV+RN WEEK+WD+D T+G+KFKEL
Sbjct: 301 NEELKKRVELQTFNARDAERMKRELQAVERDITEAEVARNGWEEKSWDLDTTIGHKFKEL 360

Query: 361 EAMARECNQAMRRLKLDNCHQYFLNAKGSTPADVMGVDYKTTIKPALDSFAEDIKKSSMT 420
           EA++ ECNQA+RRLKL N  QY LNAKGS+PA+V+G+DYK+T+KPALDSFA+DI KSSM+
Sbjct: 361 EALSIECNQALRRLKLGNGLQYVLNAKGSSPAEVLGIDYKSTLKPALDSFADDINKSSMS 420

Query: 421 KLEDLISLRQQSSENTAKIESRRSHIVSLQSHIEEVETQVNSLKNEIRDYTLRCSTESKK 480
           KLE+LISL+QQS EN AKIE++R+ + +LQS  +EVE Q+  LK E ++YT RC+TE+KK
Sbjct: 421 KLEELISLQQQSVENAAKIEAKRNRLAALQSSSDEVEAQLIFLKKESQNYTSRCATEAKK 480

Query: 481 MVEDVQLEAHSIDVVESEAGEILKNAKLKHEEAIKQGEEEILICARELVACVDSVSQYKE 540
           +VEDV++E H++D+VE E  ++L+ +KLK +EAIKQ EEEI +CA EL A VDSVS+YKE
Sbjct: 481 LVEDVEIETHNVDIVEREVADVLETSKLKLQEAIKQNEEEIQMCAWELYALVDSVSKYKE 540

Query: 541 FVRSKISDMRSNLSKTAVGISEAYKNSLPAELG 568
            + SKIS+M+ NLS+TA  +S++YK SL A+ G
Sbjct: 541 HMASKISEMKCNLSETAGAVSDSYKGSLLAQFG 564

BLAST of Cp4.1LG14g04000 vs. NCBI nr
Match: gi|147771819|emb|CAN66776.1| (hypothetical protein VITISV_035303 [Vitis vinifera])

HSP 1 Score: 612.1 bits (1577), Expect = 1.0e-171
Identity = 336/573 (58.64%), Postives = 433/573 (75.57%), Query Frame = 1

Query: 1   MKGNGRRHPKGSFNPPPPPTP------QFRRDSDASFASSRPSSIGMARPAAASIDMYKE 60
           MK  GRR PK S  PPPPP P      QF RDSDASFASSRPSS+G+ R +A   D   +
Sbjct: 1   MKATGRRRPKDSIRPPPPPAPSVDHHRQFSRDSDASFASSRPSSLGINRSSAG--DALTD 60

Query: 61  RAVQISAVNTINSSLKELFNHSFNLTFKPFSPPSLKDLTETIHLLLQCLDYHPTKLEDDL 120
           R+ Q+SA+ TINS L    +HS  L+ KP   PS KD++ET+  L+  LD+  TKLEDDL
Sbjct: 61  RSYQLSAIRTINSFLA---SHSSPLSLKP-PLPSAKDISETLKFLISQLDFPTTKLEDDL 120

Query: 121 PIVLKSLGYPFKINKGILKTPPAPHQWYPILALLHWLVQVAVYNYNLANDSDSFVSSNDV 180
           PI+LK L  P K+NK  LK P  PH W P+L ++HWLVQ+A+YN +L ++   F   ++ 
Sbjct: 121 PILLKHLNCPIKLNKSALKAPGTPHAWPPLLGVMHWLVQIALYNDHLVSNLQCF---DNT 180

Query: 181 NVYVLNSYVQYIRGDDDSVEALDQEFMERLKKGRDDAVESLNGLEMEVKELEAKAEALRS 240
            VY L+SY+ YIRGDDD +EA+D+EFM +L+  RD   E +  LE EV E E + E LRS
Sbjct: 181 FVYALDSYLHYIRGDDDGMEAVDREFMGKLEXERDAVAEGVKALEKEVAEREGRLEELRS 240

Query: 241 DPSQREVLEKQQTVLEEDVKKFHTMIAGYSEKMQEMKKILEEKEKELEVKVQENKKICEE 300
            PS +EV+EK++ VLEEDVKKFH +IA +S ++  ++KILEEKEKEL VKV+EN +ICEE
Sbjct: 241 GPSAKEVVEKERGVLEEDVKKFHAIIAEFSGRIASVEKILEEKEKELGVKVEENNRICEE 300

Query: 301 NEELKMRVESQMFNARDVERMRRELQAVERDIGDAEVSRNSWEEKTWDIDATVGNKFKEL 360
           NEELK RVE Q FNARD ERM+RELQAVERDI +AEV+RN WEEK+WD+D T+G+KFKEL
Sbjct: 301 NEELKKRVELQTFNARDAERMKRELQAVERDITEAEVARNGWEEKSWDLDTTIGHKFKEL 360

Query: 361 EAMARECNQAMRRLKLDNCHQYFLNAKGSTPADVMGVDYKTTIKPALDSFAEDIKKSSMT 420
           EA++ ECNQA+RRLKL N  QY LNAKGS+P +V+G+DYK+ +KPALDSFA+DI KSSM+
Sbjct: 361 EALSIECNQALRRLKLGNGLQYVLNAKGSSPXEVLGIDYKSXLKPALDSFADDINKSSMS 420

Query: 421 KLEDLISLRQQSSENTAKIESRRSHIVSLQSHIEEVETQVNSLKNEIRDYTLRCSTESKK 480
           KLE+LISL+QQS EN AKIE++R+ + +LQS  +EVE Q+  LK E ++YT RC+TE+KK
Sbjct: 421 KLEELISLQQQSVENAAKIEAKRNRLAALQSXSDEVEAQLIFLKKESQNYTSRCATEAKK 480

Query: 481 MVEDVQLEAHSIDVVESEAGEILKNAKLKHEEAIKQGEEEILICARELVACVDSVSQYKE 540
           +VEDV++E H++D VE E  ++L+ +KLK +EAIKQ EEEI  CA EL A VDSVS+YKE
Sbjct: 481 LVEDVEIETHNVDXVEREVADVLETSKLKLQEAIKQNEEEIQXCAWELYALVDSVSKYKE 540

Query: 541 FVRSKISDMRSNLSKTAVGISEAYKNSLPAELG 568
            + SKIS+M+ NLS+TA  +S++YK SL A+ G
Sbjct: 541 HMASKISEMKCNLSETAGAVSDSYKGSLLAQFG 564