Cp4.1LG12g00960 (gene) Cucurbita pepo (Zucchini)

Overview
NameCp4.1LG12g00960
Typegene
OrganismCucurbita pepo (Cucurbita pepo (Zucchini))
DescriptionPectinesterase
LocationCp4.1LG12 : 553363 .. 555617 (-)
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS
Hold the cursor over a type above to highlight its positions in the sequence below.
GGGAATGAAGATGAACGTTATAGAAGCGTGGGAGGGAAGAAATTGGCATGGCGGACAGTGGTTGTGGATCAGAGCGGCCATGGAAATTTCTCAACCATTCAAGCCGCCATTGATTCTGTTCCTTCAAATAATATGTTCTGGGTCTCCATTCATATCAACCCTGGTATTTACAGGTCATTTTCAATTTCCTTAATTACCCTCAATTTCTTTTCTTATTTATTTATTTATTTTTTTATTTATTTGTTTGTTTGTTCTTGTTTAGAGAGAAGGTGAAAATCCCATATGATAAGCCGTATATCATCTTGAAAGGACAAAGAAGAAAAACAACAAAGGTTGTCTGGAACGACCATTTCACTCTTGCTCAAAGTCCTACTTTCACTTCTTCAGCTGATAATGTTGTCGTTAAATCCATCACTTTTGTGGTAAGTAAGTGAACATCATTCATTTCTTCATATTAACTCTGTTTTTTTTTTTGTGATCTAATTCTTGTGGGTATTAGAATTCGTTTAACTTTCCATGGACTAATGGAAATCGAAGAGCTCCAGCAGTTGCAGCTATGATCACTGGCGATAAGGCTTTGTTTTATAAATGTGGCTTCTATGGTGTCCAAGATACCTTGTGGGATGATCAAGGTCGACATTATTATCATCGTTGCGTCATTCAAGGTGCTGTCGATTTTATTTTCGGAAGCGCTCAATCCATTTTTCAGGTATCTTTTACTGTTTCGATATGACGTTTGAAGCAACATAGGTAGCGGGAACTTACTAGGGTTTAGGGTTTAGGGTTTAGAGTGAGCTTGTGTAACAGCCCAGATCCACCCCTAGCAAATATTGTCCTCTTTGGGCTTTTACTTTCGGGCTTCTCCTCAAGACTTTAAAATGCGTCTGTTGGGGATATGATGTTTGAAGCAAAATAGGTGGCAAGAACTTACTAGGGTTTAGGGTGAGACTGTGCTCGGATAAAGATGAAGTCATGAAACCCTAATCGTTTTTTATGATGTCGAAGGATATGATGTTTGAATCAACATAGGTGATAGGAACTTACTAGGGTTTAGGGTTTAGGGTGAGACTGTGTTCGGGTAAAGATGAAGTCGTGAAACCTTAATCGATGTCAAATGGTATGACATTTGAAGCAACATAGGTGCCGGGAACTTACTAGAGTTTAGGATGAGCCTGTGGTCGGTTAAAGATGAAGTTGTGAAACCCGAATCGTTTTCGTTGATGTAGGGATGTTCGATATCAGTGGTGGGAGAAGCACTTCTTCCATTGGGGTCTACAAGCTTCATAACAGCACAAGGAAGAACAGACCCAAATGATGCAAATGGGTTTGTTTTCAAAGATTGCAAAGTGTTTGGGAGTGGCTCAGCTTTCTTAGGAAGGCCATGGAGAGGCTACTCAAGGGTTATCTTTTACAATTCTAGCTTCTCCAACGTCATAAACCCTAGTGGTTGGGATCCTTGGCATTTTGTTGGTCATGAGTAAGCTATCTTTTATTCTTCAACGGTTTATCGGCTCGACTTTTCAAAAGTTACAATATTGCCCTTTGACCTTTCATAAATACTTCAAAATTGTATTATCAAGGTCGAGGATAATATTGTAACTTTCAAAAAAACCCATAGCGTTTCTTTCTCTACTAATAGCTACAAAACCTTGCTTTCAAACTCTCTTTCGAAAATCTCTCTGCCCCAATTTTCTAAAACTTTCTTCTCAAGACGGGGTTAGAGGCTTGAGCATACGTTGCTCGACCGTAGGCGACGCAAATTCTAATTGGCTTGACTCACTCAGTGAGTGCGACACCGTTACAAATGTCCATTGCCATATATATAATATATATTGGTTTATACTAGAAAAAGAATATAATTAATGACTTACTTTCTTTGTAAGAAAAATAGGCGTTTACTAATAATTTCCTTTTTAGCTTTTATCCATTTTTAAAATCTTAGGGTTTTGTCCATATAAATCCTAAATTTAAAAAAATAAAAATAAAAATTTTGCATGAAAAAAAAGACTTATTAGGAAAGAAAATAAAGTTGTTTAAAGTATTTGTAATTACTATTTTTTATTATTATTATAATGAATATTTTATTTTTATAATCCAGGGACCAATTAACCTATGCAGAGTATGGTTGCTACGGACCAGGTTCCGACATGTCGCATCGAGTAAAATGGGAAAAGAAGCTGGATTTGGATGAAATACAACAGCTAACCGACATGAGTTTTATCGATACTGAAGGTTGGATGCAAGATCAACCTTGG

mRNA sequence

GGGAATGAAGATGAACGTTATAGAAGCGTGGGAGGGAAGAAATTGGCATGGCGGACAGTGGTGAAAATCCCATATGATAAGCCGTATATCATCTTGAAAGGACAAAGAAGAAAAACAACAAAGGTTGTCTGGAACGACCATTTCACTCTTGCTCAAAGTCCTACTTTCACTTCTTCAGCTGATAATGTTGTCGTTAAATCCATCACTTTTGTGAATTCGTTTAACTTTCCATGGACTAATGGAAATCGAAGAGCTCCAGCAGTTGCAGCTATGATCACTGGCGATAAGGCTTTGTTTTATAAATGTGGCTTCTATGGTGTCCAAGATACCTTGTGGGATGATCAAGGTCGACATTATTATCATCGTTGCGTCATTCAAGTGGTGGGAGAAGCACTTCTTCCATTGGGGTCTACAAGCTTCATAACAGCACAAGGAAGAACAGACCCAAATGATGCAAATGGGTTTGTTTTCAAAGATTGCAAAGTGTTTGGGAGTGGCTCAGCTTTCTTAGGAAGGCCATGGAGAGGCTACTCAAGGGTTATCTTTTACAATTCTAGCTTCTCCAACGTCATAAACCCTAGTGGTTGGGATCCTTGGCATTTTCTTTTATCCATTTTTAAAATCTTAGGGGACCAATTAACCTATGCAGAGTATGGTTGCTACGGACCAGGTTCCGACATGTCGCATCGAGTAAAATGGGAAAAGAAGCTGGATTTGGATGAAATACAACAGCTAACCGACATGAGTTTTATCGATACTGAAGGTTGGATGCAAGATCAACCTTGG

Coding sequence (CDS)

GGGAATGAAGATGAACGTTATAGAAGCGTGGGAGGGAAGAAATTGGCATGGCGGACAGTGGTGAAAATCCCATATGATAAGCCGTATATCATCTTGAAAGGACAAAGAAGAAAAACAACAAAGGTTGTCTGGAACGACCATTTCACTCTTGCTCAAAGTCCTACTTTCACTTCTTCAGCTGATAATGTTGTCGTTAAATCCATCACTTTTGTGAATTCGTTTAACTTTCCATGGACTAATGGAAATCGAAGAGCTCCAGCAGTTGCAGCTATGATCACTGGCGATAAGGCTTTGTTTTATAAATGTGGCTTCTATGGTGTCCAAGATACCTTGTGGGATGATCAAGGTCGACATTATTATCATCGTTGCGTCATTCAAGTGGTGGGAGAAGCACTTCTTCCATTGGGGTCTACAAGCTTCATAACAGCACAAGGAAGAACAGACCCAAATGATGCAAATGGGTTTGTTTTCAAAGATTGCAAAGTGTTTGGGAGTGGCTCAGCTTTCTTAGGAAGGCCATGGAGAGGCTACTCAAGGGTTATCTTTTACAATTCTAGCTTCTCCAACGTCATAAACCCTAGTGGTTGGGATCCTTGGCATTTTCTTTTATCCATTTTTAAAATCTTAGGGGACCAATTAACCTATGCAGAGTATGGTTGCTACGGACCAGGTTCCGACATGTCGCATCGAGTAAAATGGGAAAAGAAGCTGGATTTGGATGAAATACAACAGCTAACCGACATGAGTTTTATCGATACTGAAGGTTGGATGCAAGATCAACCTTGG

Protein sequence

GNEDERYRSVGGKKLAWRTVVKIPYDKPYIILKGQRRKTTKVVWNDHFTLAQSPTFTSSADNVVVKSITFVNSFNFPWTNGNRRAPAVAAMITGDKALFYKCGFYGVQDTLWDDQGRHYYHRCVIQVVGEALLPLGSTSFITAQGRTDPNDANGFVFKDCKVFGSGSAFLGRPWRGYSRVIFYNSSFSNVINPSGWDPWHFLLSIFKILGDQLTYAEYGCYGPGSDMSHRVKWEKKLDLDEIQQLTDMSFIDTEGWMQDQPW
Homology
[Close]
BLAST of Cp4.1LG12g00960 vs. Swiss-Prot
Match: PME29_ARATH (Probable pectinesterase 29 OS=Arabidopsis thaliana GN=PME29 PE=2 SV=1)

HSP 1 Score: 299.7 bits (766), Expect = 3.2e-80
Identity = 143/268 (53.36%), Postives = 186/268 (69.40%), Query Frame = 1 

Query: 17  WRTVVKIPYDKPYIILKGQRRKTTKVVWNDHFTLAQSPTFTSSADNVVVKSITFVNSFNF 76
           +R  +KIPY+KP+I+L G  ++ T+V W+DH+++AQSPTF++ ADN VVKSITF NS+NF
Sbjct: 71  YREKIKIPYEKPFIVLVGAGKRLTRVEWDDHYSVAQSPTFSTLADNTVVKSITFANSYNF 130

Query: 77  PWT---NGNRRAPAVAAMITGDKALFYKCGFYGVQDTLWDDQGRHYYHRC---------- 136
           P     N N R PAVAA+I GDK+ FY  GF G+QDTLWD  GRHY+HRC          
Sbjct: 131 PSKGKMNKNPRTPAVAALIGGDKSAFYSVGFAGIQDTLWDFDGRHYFHRCTIQGAVDFIF 190

Query: 137 ----------VIQVVGEALLPLGSTSFITAQGRTDPNDANGFVFKDCKVFGSGSAFLGRP 196
                     VIQV+G  L P G   +ITAQGRT+P DANGF+F +C V+G+G AFLGRP
Sbjct: 191 GTGQSIYQSCVIQVLGGQLEP-GLAGYITAQGRTNPYDANGFIFINCLVYGTGMAFLGRP 250

Query: 197 WRGYSRVIFYNSSFSNVINPSGWDPWHFLLSIFKILGDQLTYAEYGCYGPGSDMSHRVKW 256
           WRGYSRVIFYNS+ ++V+ P GWD W+F+        +QL +AE+GC+G G+++  RVKW
Sbjct: 251 WRGYSRVIFYNSNLTDVVVPEGWDAWNFVGH-----ENQLVFAEHGCFGSGANIGRRVKW 310

Query: 257 EKKLDLDEIQQLTDMSFIDTEGWMQDQP 262
            KKL    IQ L D+SFI+  GW++D P
Sbjct: 311 VKKLSESAIQNLADLSFINRGGWVEDLP 332

[Close]
BLAST of Cp4.1LG12g00960 vs. Swiss-Prot
Match: PME55_ARATH (Probable pectinesterase 55 OS=Arabidopsis thaliana GN=PME55 PE=2 SV=1)

HSP 1 Score: 283.1 bits (723), Expect = 3.1e-75
Identity = 139/268 (51.87%), Postives = 176/268 (65.67%), Query Frame = 1 

Query: 17  WRTVVKIPYDKPYIILKGQRRKTTKVVWNDHFTLAQSPTFTSSADNVVVKSITFVNSFNF 76
           +R  + IP  KP+I++ G  +++T+V W+DH +LAQSPTF + ADN VVK ITF NS+NF
Sbjct: 66  YREKITIPQKKPFIVIVGAGKRSTRVEWDDHASLAQSPTFATLADNTVVKKITFANSYNF 125

Query: 77  PWT---NGNRRAPAVAAMITGDKALFYKCGFYGVQDTLWDDQGRHYYHRC---------- 136
           P     N N R PAVAA I GDK+ FY  GF G+QDTLWD  GRHY+HRC          
Sbjct: 126 PSNGKINKNPRVPAVAAFIGGDKSAFYSVGFAGIQDTLWDSDGRHYFHRCTIQGAVDFIL 185

Query: 137 ----------VIQVVGEALLPLGSTSFITAQGRTDPNDANGFVFKDCKVFGSGSAFLGRP 196
                     VIQV+G  L P G T +ITAQGRT+ NDANGFVF +C V G G A+LGR 
Sbjct: 186 GSGQSIYQSCVIQVLGGQLGP-GVTGYITAQGRTNANDANGFVFINCLVHGFGKAYLGRA 245

Query: 197 WRGYSRVIFYNSSFSNVINPSGWDPWHFLLSIFKILGDQLTYAEYGCYGPGSDMSHRVKW 256
           WR YSRVIFYNS+ ++V++P GW  W+     ++    QLTYAE+GC+G GS+ S R KW
Sbjct: 246 WRPYSRVIFYNSNLTDVVDPLGWWEWN-----YQGYEKQLTYAEHGCFGSGSNTSRRAKW 305

Query: 257 EKKLDLDEIQQLTDMSFIDTEGWMQDQP 262
            KKL    +Q L D+SFI+  GW++D P
Sbjct: 306 VKKLSASAVQHLADLSFINRGGWVEDLP 327

[Close]
BLAST of Cp4.1LG12g00960 vs. Swiss-Prot
Match: PME10_ARATH (Putative pectinesterase 10 OS=Arabidopsis thaliana GN=PME10 PE=2 SV=1)

HSP 1 Score: 205.7 bits (522), Expect = 6.3e-52
Identity = 111/263 (42.21%), Postives = 141/263 (53.61%), Query Frame = 1 

Query: 21  VKIPYDKPYIILKGQRRKTTKVVWNDHFTLAQSPTFTSSADNVVVKSITFVNSFNFPWTN 80
           V IP  K YI ++G   + T + + DH     S TFTS   N+++  ITF N +N   ++
Sbjct: 82  VTIPRGKGYIYMQGGGIEKTIIAYGDHQLTNTSATFTSYPSNIIITGITFKNKYNIA-SS 141

Query: 81  GNRRAPAVAAMITGDKALFYKCGFYGVQDTLWDDQGRHYYHRCVIQVVGEALLPLGSTSF 140
            +   PAVAAM+ GDK       F G QDTL+DD GRHYY RCVI    + +     + F
Sbjct: 142 SSPTKPAVAAMMLGDKYAIIDSSFDGFQDTLYDDYGRHYYKRCVISGGIDFIFGGAQSIF 201

Query: 141 --------------------ITAQGRTDPNDANGFVFKDCKVFGSGSAFLGRPWRGYSRV 200
                               ITAQGR  P D  GFVFKDC V GSG A LGR W+ YSRV
Sbjct: 202 EGCTLKLRVGIYPPNEVYGTITAQGRDSPTDKGGFVFKDCTVMGSGKALLGRAWKSYSRV 261

Query: 201 IFYNSSFSNVINPSGWDPWHFLLSIFKILGDQ--LTYAEYGCYGPGSDMSHRVKWEKKLD 260
           IFY S FS+ I P GWD W       K  G +  +T+ E+GC G G+D S RV W  K  
Sbjct: 262 IFYRSMFSDNILPIGWDAW-------KAKGQEGHITFVEFGCTGVGADTSKRVPWLTKAS 321

Query: 261 LDEIQQLTDMSFIDTEGWMQDQP 262
             ++ Q T+++FID EGW+   P
Sbjct: 322 EKDVLQFTNLTFIDEEGWLSRLP 336

[Close]
BLAST of Cp4.1LG12g00960 vs. Swiss-Prot
Match: PME66_ARATH (Probable pectinesterase 66 OS=Arabidopsis thaliana GN=PME66 PE=2 SV=2)

HSP 1 Score: 194.1 bits (492), Expect = 1.9e-48
Identity = 104/267 (38.95%), Postives = 142/267 (53.18%), Query Frame = 1 

Query: 17  WRTVVKIPYDKPYIILKGQRRKTTKVVWNDHFTLAQSPTFTSSADNVVVKSITFVNSFNF 76
           +R  V IP +K +I L+G+  + T + ++DH     S TFT+ AD++V+  ITF N++N 
Sbjct: 72  YREKVTIPKEKGFIYLQGKGIEQTVIEYDDHQATDISATFTAFADDIVISGITFKNTYNI 131

Query: 77  PWTNGNRRAPAVAAMITGDKALFYKCGFYGVQDTLWDDQGRHYYHRCVIQVVGEALLPLG 136
              N     PAVAA + GD+ +     F G+QDTL+D +GRHYY RC+I    + +   G
Sbjct: 132 VPNNKREIVPAVAARMLGDRYVVTDSSFVGLQDTLFDGKGRHYYKRCIISGGIDFIFGYG 191

Query: 137 STSF--------------------ITAQGRTDPNDANGFVFKDCKVFGSGSAFLGRPWRG 196
            + F                    ITA  R  P+D  GFVF DC V G G   LGR W  
Sbjct: 192 QSLFKECTLNMTLGIYAPDNPYGTITAHQRPSPSDEGGFVFSDCTVTGVGKTLLGRAWGS 251

Query: 197 YSRVIFYNSSFSNVINPSGWDPWHFLLSIFKILGDQ--LTYAEYGCYGPGSDMSHRVKWE 256
            +RVIF  S  S+V+ P GWD W       +  G++  LT+ E GC G G+D S RV W 
Sbjct: 252 NARVIFDRSRLSDVVLPIGWDAW-------RAKGNERDLTFVEAGCTGAGADTSQRVPWL 311

Query: 257 KKLDLDEIQQLTDMSFIDTEGWMQDQP 262
           KKL L E+     +SFID +GW+   P
Sbjct: 312 KKLSLSEVDGFASVSFIDQDGWISRFP 331

[Close]
BLAST of Cp4.1LG12g00960 vs. Swiss-Prot
Match: PME11_ARATH (Putative pectinesterase 11 OS=Arabidopsis thaliana GN=PME11 PE=3 SV=1)

HSP 1 Score: 184.5 bits (467), Expect = 1.5e-45
Identity = 101/264 (38.26%), Postives = 133/264 (50.38%), Query Frame = 1 

Query: 17  WRTVVKIPYDKPYIILKGQRRKTTKVVWNDHFTLAQSPTFTSSADNVVVKSITFVNSFNF 76
           +R  V IP +KPYI L G +   T ++W+D   + +SPT T  A + V + +T  N F  
Sbjct: 90  YREKVVIPAEKPYITLSGTQASNTFLIWSDGEDILESPTLTIFASDFVCRFLTIQNKFG- 149

Query: 77  PWTNGNRRAPAVAAMITGDKALFYKCGFYGVQDTLWDDQGRHYYHRCVIQVVGEALLPLG 136
             T G     AVA  +  DKA FY C     QDTL DD G HY+  C I+   + +    
Sbjct: 150 --TAGR----AVALRVAADKAAFYGCVITSYQDTLLDDNGNHYFKNCYIEGATDFICGSA 209

Query: 137 STSF--------------ITAQGRTDPNDANGFVFKDCKVFGSGSAFLGRPWRGYSRVIF 196
           S+ +              ITAQ RT   + +GF F  CK+ GSGS FLGRPW  YSRV+F
Sbjct: 210 SSLYERCHLHSLSPNNGSITAQMRTSATEKSGFTFLGCKLTGSGSTFLGRPWGAYSRVVF 269

Query: 197 YNSSFSNVINPSGWDPWHFLLSIFKILGDQ-----LTYAEYGCYGPGSDMSHRVKWEKKL 256
             S FSNV+ P GW+ W          GD      + Y EY CYGPG+D   RV+W K+L
Sbjct: 270 AYSFFSNVVAPQGWNQW----------GDSTKENTVYYGEYKCYGPGADREQRVEWSKQL 329

Query: 257 DLDEIQQLTDMSFIDTEGWMQDQP 262
             +E        FI  + W++  P
Sbjct: 330 SDEEATVFLSKDFIGGKDWLRPAP 336

[Close]
BLAST of Cp4.1LG12g00960 vs. TrEMBL
Match: W9SM62_9ROSA (Pectinesterase OS=Morus notabilis GN=L484_021593 PE=4 SV=1)

HSP 1 Score: 338.2 bits (866), Expect = 9.0e-90
Identity = 164/266 (61.65%), Postives = 194/266 (72.93%), Query Frame = 1 

Query: 17  WRTVVKIPYDKPYIILKGQRRKTTKVVWNDHFTLAQSPTFTSSADNVVVKSITFVNSFNF 76
           +R  +KIPYDKPYIILKG+ R+ T+VVW+DH TLAQSPTFTS ADN+V K ++F NS+N 
Sbjct: 63  YREKIKIPYDKPYIILKGEGRRKTQVVWDDHDTLAQSPTFTSLADNIVAKCMSFTNSYNR 122

Query: 77  PWTNGNRRAPAVAAMITGDKALFYKCGFYGVQDTLWDDQGRHYY---------------- 136
           P  N   RAPA AAMITGDK  F++CGF+G+QDTLWDDQGRHYY                
Sbjct: 123 P-PNDKPRAPAAAAMITGDKTKFFRCGFFGLQDTLWDDQGRHYYKLCTIQGAVDFIFGSG 182

Query: 137 ----HRCVIQVVGEALLPLGSTSFITAQGRTDPNDANGFVFKDCKVFGSGSAFLGRPWRG 196
                RC I VVG AL P G   FITAQGRT+PNDANGFVFKDC VFGSGSAFLGRPWRG
Sbjct: 183 QSIFERCSISVVGGALEP-GFQGFITAQGRTNPNDANGFVFKDCHVFGSGSAFLGRPWRG 242

Query: 197 YSRVIFYNSSFSNVINPSGWDPWHFLLSIFKILGDQLTYAEYGCYGPGSDMSHRVKWEKK 256
           Y+RV FYN++ S+VI P GW+ W+F    +     QLTYAE GC+GPGSD S RV WEKK
Sbjct: 243 YARVFFYNTNISDVIVPQGWNAWNFAGQEY-----QLTYAEEGCFGPGSDSSRRVSWEKK 302

Query: 257 LDLDEIQQLTDMSFIDTEGWMQDQPW 263
           L+ + ++QLT  SFID EGW+ +QP+
Sbjct: 303 LNEETVKQLTSSSFIDDEGWLNNQPF 321

[Close]
BLAST of Cp4.1LG12g00960 vs. TrEMBL
Match: M5Y357_PRUPE (Pectinesterase OS=Prunus persica GN=PRUPE_ppa020682mg PE=4 SV=1)

HSP 1 Score: 336.3 bits (861), Expect = 3.4e-89
Identity = 160/274 (58.39%), Postives = 199/274 (72.63%), Query Frame = 1 

Query: 9   SVGGKKLAWRTVVKIPYDKPYIILKGQRRKTTKVVWNDHFTLAQSPTFTSSADNVVVKSI 68
           S+  K   ++  V IP DKPYIILKG+ R  T++VW+DH ++AQSPTF S AD+++VKSI
Sbjct: 45  SIKIKAGTYKEKVIIPVDKPYIILKGENRHKTQIVWDDHDSVAQSPTFASYADSIIVKSI 104

Query: 69  TFVNSFNFPWTNGNRRAPAVAAMITGDKALFYKCGFYGVQDTLWDDQGRHYYH------- 128
           +FVNS+N P  N N R  AVAAMI GDK+ FY+CGF+G+QDTLWD QGRHYYH       
Sbjct: 105 SFVNSYNNPVNNKNPRVAAVAAMIYGDKSSFYRCGFFGLQDTLWDGQGRHYYHLCTIQGA 164

Query: 129 -------------RCVIQVVGEALLPLGSTSFITAQGRTDPNDANGFVFKDCKVFGSGSA 188
                        +C IQV+G AL P GS  +ITAQGR +PNDA+GFVFKDCKV G+GS 
Sbjct: 165 VDFIFGSAQSIFQKCSIQVLGGALDP-GSAGYITAQGRDNPNDASGFVFKDCKVSGTGST 224

Query: 189 FLGRPWRGYSRVIFYNSSFSNVINPSGWDPWHFLLSIFKILGDQLTYAEYGCYGPGSDMS 248
           FLGR WRGYSRVIFYNS+FS V+ P GWD WHF  +       QLTYAE+GCYGPG+D  
Sbjct: 225 FLGRAWRGYSRVIFYNSNFSQVVVPQGWDAWHFQGN-----EHQLTYAEHGCYGPGADTV 284

Query: 249 HRVKWEKKLDLDEIQQLTDMSFIDTEGWMQDQPW 263
            RV+WEKKL+ D +++LT ++FIDT+GW+ DQP+
Sbjct: 285 KRVEWEKKLNADTVRELTSLNFIDTDGWLNDQPF 312

[Close]
BLAST of Cp4.1LG12g00960 vs. TrEMBL
Match: A0A061ELR2_THECC (Pectinesterase OS=Theobroma cacao GN=TCM_020334 PE=4 SV=1)

HSP 1 Score: 325.5 bits (833), Expect = 6.0e-86
Identity = 153/265 (57.74%), Postives = 193/265 (72.83%), Query Frame = 1 

Query: 17  WRTVVKIPYDKPYIILKGQRRKTTKVVWNDHFTLAQSPTFTSSADNVVVKSITFVNSFNF 76
           +R  VKIPYDKP+IILKG  +++T V+W+DH +L QSPTF+S ADN+VV+S++FVNS+N 
Sbjct: 68  YREKVKIPYDKPFIILKGAGKRSTSVIWDDHQSLLQSPTFSSLADNIVVESMSFVNSYNS 127

Query: 77  PWTNGNRRAPAVAAMITGDKALFYKCGFYGVQDTLWDDQGRHYYHRCVIQ---------- 136
           P  + N R PAVAAM++GDK+ FYKCGF  +QDTLWDDQGRHY+ RC I+          
Sbjct: 128 P-NSKNPRVPAVAAMVSGDKSFFYKCGFLSLQDTLWDDQGRHYFKRCTIEGAVDFIFGSG 187

Query: 137 ----------VVGEALLPLGSTSFITAQGRTDPNDANGFVFKDCKVFGSGSAFLGRPWRG 196
                      +G  L P G   FITAQGRT+PNDANGFVFK+C V+GSG+ FLGRPWRG
Sbjct: 188 QSIYEDSAIHFLGGELEP-GLPGFITAQGRTNPNDANGFVFKNCNVYGSGTTFLGRPWRG 247

Query: 197 YSRVIFYNSSFSNVINPSGWDPWHFLLSIFKILGDQLTYAEYGCYGPGSDMSHRVKWEKK 256
           YSRV+FYN  FS++I P GW+ W F         +QLT+AEYG +GPGSD S RV WEKK
Sbjct: 248 YSRVLFYNCKFSDIIQPGGWEAWSFTGH-----ENQLTFAEYGNHGPGSDTSKRVSWEKK 307

Query: 257 LDLDEIQQLTDMSFIDTEGWMQDQP 262
           L ++ ++QLT M+FID+EGW+Q QP
Sbjct: 308 LSVEAVEQLTSMTFIDSEGWLQSQP 325

[Close]
BLAST of Cp4.1LG12g00960 vs. TrEMBL
Match: B9RWI7_RICCO (Pectinesterase OS=Ricinus communis GN=RCOM_1019830 PE=4 SV=1)

HSP 1 Score: 321.6 bits (823), Expect = 8.7e-85
Identity = 150/257 (58.37%), Postives = 189/257 (73.54%), Query Frame = 1 

Query: 17  WRTVVKIPYDKPYIILKGQRRKTTKVVWNDHFTLAQSPTFTSSADNVVVKSITFVNSFNF 76
           +R  VKIPYD+PYIILKG+ ++ T+++W+DH + AQSPTF S ADN++VKSI FVNS+NF
Sbjct: 85  YREKVKIPYDRPYIILKGEAKRRTQIIWDDHDSTAQSPTFMSLADNIIVKSIRFVNSYNF 144

Query: 77  PWTNGNRRAPAVAAMITGDKALFYKCGFYGVQDTLWDDQGRHYYHRCVIQVVGEALLPLG 136
             +N N R PAVAAMI GDK+ FY+CGF GVQDTLWDDQGRHY+ +C IQ   + +   G
Sbjct: 145 LNSN-NPRVPAVAAMIAGDKSAFYRCGFAGVQDTLWDDQGRHYFKKCTIQGAVDFIFGSG 204

Query: 137 ST------------SFITAQGRTDPNDANGFVFKDCKVFGSGSAFLGRPWRGYSRVIFYN 196
            +             FITAQGRT+P+DANGFVFK C VFG GSA+LGRPWRGYSRV+FY 
Sbjct: 205 QSIYEGCAIQVIGDGFITAQGRTNPSDANGFVFKRCNVFGRGSAYLGRPWRGYSRVLFYQ 264

Query: 197 SSFSNVINPSGWDPWHFLLSIFKILGDQLTYAEYGCYGPGSDMSHRVKWEKKLDLDEIQQ 256
           S+F+NVI+P GW+ W F+        +Q+T+AEYG +GPG+D  +RV W KKL    + +
Sbjct: 265 SNFTNVIHPEGWNAWDFVHH-----ENQITFAEYGNFGPGADTKNRVSWAKKLSHQTLCK 324

Query: 257 LTDMSFIDTEGWMQDQP 262
           L  MSFIDTE W+QDQP
Sbjct: 325 LVSMSFIDTENWIQDQP 335

[Close]
BLAST of Cp4.1LG12g00960 vs. TrEMBL
Match: A0A059CS97_EUCGR (Pectinesterase (Fragment) OS=Eucalyptus grandis GN=EUGRSUZ_C02449 PE=4 SV=1)

HSP 1 Score: 319.3 bits (817), Expect = 4.3e-84
Identity = 151/269 (56.13%), Postives = 190/269 (70.63%), Query Frame = 1 

Query: 13  KKLAWRTVVKIPYDKPYIILKGQRRKTTKVVWNDHFTLAQSPTFTSSADNVVVKSITFVN 72
           KK  +R  VKIP DKPYIILKGQ ++TT++VW+DH  + QSPTFTS ADN++ KSI+F N
Sbjct: 43  KKGIYREKVKIPEDKPYIILKGQAKRTTEIVWDDHDNIVQSPTFTSLADNIIAKSISFRN 102

Query: 73  SFNFPWTNGNRRAPAVAAMITGDKALFYKCGFYGVQDTLWDDQGRHYYHRCVIQ------ 132
           S+N P    + R PAVAA I+GDK +F++CGFYG+QDTLWDDQGRHY+ RC +Q      
Sbjct: 103 SYNNPINKKHPRVPAVAARISGDKNVFHRCGFYGLQDTLWDDQGRHYFKRCTVQGAVDFI 162

Query: 133 --------------VVGEALLPLGSTSFITAQGRTDPNDANGFVFKDCKVFGSGSAFLGR 192
                         V+G AL   G T FITAQGR++P+DA+GFVFK+C VFGSGSA LGR
Sbjct: 163 FGGGQSIYENCAISVLGGAL-GSGVTGFITAQGRSNPDDASGFVFKECNVFGSGSALLGR 222

Query: 193 PWRGYSRVIFYNSSFSNVINPSGWDPWHFLLSIFKILGDQLTYAEYGCYGPGSDMSHRVK 252
            WR Y+RVIFY S+F++V+NP GWD W+F          QLT++EY CYGPGS  S R  
Sbjct: 223 AWRAYARVIFYRSNFTDVVNPRGWDAWNFAGQ-----ESQLTFSEYECYGPGSGTSLRAS 282

Query: 253 WEKKLDLDEIQQLTDMSFIDTEGWMQDQP 262
           WEKKL++  + QLT  SF+D+EGW+  QP
Sbjct: 283 WEKKLNVATVNQLTSTSFVDSEGWLSSQP 305

[Close]
BLAST of Cp4.1LG12g00960 vs. TAIR10
Match: AT3G24130.1 (AT3G24130.1 Pectin lyase-like superfamily protein)

HSP 1 Score: 299.7 bits (766), Expect = 1.8e-81
Identity = 143/268 (53.36%), Postives = 186/268 (69.40%), Query Frame = 1 

Query: 17  WRTVVKIPYDKPYIILKGQRRKTTKVVWNDHFTLAQSPTFTSSADNVVVKSITFVNSFNF 76
           +R  +KIPY+KP+I+L G  ++ T+V W+DH+++AQSPTF++ ADN VVKSITF NS+NF
Sbjct: 71  YREKIKIPYEKPFIVLVGAGKRLTRVEWDDHYSVAQSPTFSTLADNTVVKSITFANSYNF 130

Query: 77  PWT---NGNRRAPAVAAMITGDKALFYKCGFYGVQDTLWDDQGRHYYHRC---------- 136
           P     N N R PAVAA+I GDK+ FY  GF G+QDTLWD  GRHY+HRC          
Sbjct: 131 PSKGKMNKNPRTPAVAALIGGDKSAFYSVGFAGIQDTLWDFDGRHYFHRCTIQGAVDFIF 190

Query: 137 ----------VIQVVGEALLPLGSTSFITAQGRTDPNDANGFVFKDCKVFGSGSAFLGRP 196
                     VIQV+G  L P G   +ITAQGRT+P DANGF+F +C V+G+G AFLGRP
Sbjct: 191 GTGQSIYQSCVIQVLGGQLEP-GLAGYITAQGRTNPYDANGFIFINCLVYGTGMAFLGRP 250

Query: 197 WRGYSRVIFYNSSFSNVINPSGWDPWHFLLSIFKILGDQLTYAEYGCYGPGSDMSHRVKW 256
           WRGYSRVIFYNS+ ++V+ P GWD W+F+        +QL +AE+GC+G G+++  RVKW
Sbjct: 251 WRGYSRVIFYNSNLTDVVVPEGWDAWNFVGH-----ENQLVFAEHGCFGSGANIGRRVKW 310

Query: 257 EKKLDLDEIQQLTDMSFIDTEGWMQDQP 262
            KKL    IQ L D+SFI+  GW++D P
Sbjct: 311 VKKLSESAIQNLADLSFINRGGWVEDLP 332

[Close]
BLAST of Cp4.1LG12g00960 vs. TAIR10
Match: AT5G18990.1 (AT5G18990.1 Pectin lyase-like superfamily protein)

HSP 1 Score: 283.1 bits (723), Expect = 1.7e-76
Identity = 139/268 (51.87%), Postives = 176/268 (65.67%), Query Frame = 1 

Query: 17  WRTVVKIPYDKPYIILKGQRRKTTKVVWNDHFTLAQSPTFTSSADNVVVKSITFVNSFNF 76
           +R  + IP  KP+I++ G  +++T+V W+DH +LAQSPTF + ADN VVK ITF NS+NF
Sbjct: 66  YREKITIPQKKPFIVIVGAGKRSTRVEWDDHASLAQSPTFATLADNTVVKKITFANSYNF 125

Query: 77  PWT---NGNRRAPAVAAMITGDKALFYKCGFYGVQDTLWDDQGRHYYHRC---------- 136
           P     N N R PAVAA I GDK+ FY  GF G+QDTLWD  GRHY+HRC          
Sbjct: 126 PSNGKINKNPRVPAVAAFIGGDKSAFYSVGFAGIQDTLWDSDGRHYFHRCTIQGAVDFIL 185

Query: 137 ----------VIQVVGEALLPLGSTSFITAQGRTDPNDANGFVFKDCKVFGSGSAFLGRP 196
                     VIQV+G  L P G T +ITAQGRT+ NDANGFVF +C V G G A+LGR 
Sbjct: 186 GSGQSIYQSCVIQVLGGQLGP-GVTGYITAQGRTNANDANGFVFINCLVHGFGKAYLGRA 245

Query: 197 WRGYSRVIFYNSSFSNVINPSGWDPWHFLLSIFKILGDQLTYAEYGCYGPGSDMSHRVKW 256
           WR YSRVIFYNS+ ++V++P GW  W+     ++    QLTYAE+GC+G GS+ S R KW
Sbjct: 246 WRPYSRVIFYNSNLTDVVDPLGWWEWN-----YQGYEKQLTYAEHGCFGSGSNTSRRAKW 305

Query: 257 EKKLDLDEIQQLTDMSFIDTEGWMQDQP 262
            KKL    +Q L D+SFI+  GW++D P
Sbjct: 306 VKKLSASAVQHLADLSFINRGGWVEDLP 327

[Close]
BLAST of Cp4.1LG12g00960 vs. TAIR10
Match: AT2G19150.1 (AT2G19150.1 Pectin lyase-like superfamily protein)

HSP 1 Score: 205.7 bits (522), Expect = 3.5e-53
Identity = 111/263 (42.21%), Postives = 141/263 (53.61%), Query Frame = 1 

Query: 21  VKIPYDKPYIILKGQRRKTTKVVWNDHFTLAQSPTFTSSADNVVVKSITFVNSFNFPWTN 80
           V IP  K YI ++G   + T + + DH     S TFTS   N+++  ITF N +N   ++
Sbjct: 82  VTIPRGKGYIYMQGGGIEKTIIAYGDHQLTNTSATFTSYPSNIIITGITFKNKYNIA-SS 141

Query: 81  GNRRAPAVAAMITGDKALFYKCGFYGVQDTLWDDQGRHYYHRCVIQVVGEALLPLGSTSF 140
            +   PAVAAM+ GDK       F G QDTL+DD GRHYY RCVI    + +     + F
Sbjct: 142 SSPTKPAVAAMMLGDKYAIIDSSFDGFQDTLYDDYGRHYYKRCVISGGIDFIFGGAQSIF 201

Query: 141 --------------------ITAQGRTDPNDANGFVFKDCKVFGSGSAFLGRPWRGYSRV 200
                               ITAQGR  P D  GFVFKDC V GSG A LGR W+ YSRV
Sbjct: 202 EGCTLKLRVGIYPPNEVYGTITAQGRDSPTDKGGFVFKDCTVMGSGKALLGRAWKSYSRV 261

Query: 201 IFYNSSFSNVINPSGWDPWHFLLSIFKILGDQ--LTYAEYGCYGPGSDMSHRVKWEKKLD 260
           IFY S FS+ I P GWD W       K  G +  +T+ E+GC G G+D S RV W  K  
Sbjct: 262 IFYRSMFSDNILPIGWDAW-------KAKGQEGHITFVEFGCTGVGADTSKRVPWLTKAS 321

Query: 261 LDEIQQLTDMSFIDTEGWMQDQP 262
             ++ Q T+++FID EGW+   P
Sbjct: 322 EKDVLQFTNLTFIDEEGWLSRLP 336

[Close]
BLAST of Cp4.1LG12g00960 vs. TAIR10
Match: AT2G47280.1 (AT2G47280.1 Pectin lyase-like superfamily protein)

HSP 1 Score: 194.1 bits (492), Expect = 1.1e-49
Identity = 104/267 (38.95%), Postives = 142/267 (53.18%), Query Frame = 1 

Query: 17  WRTVVKIPYDKPYIILKGQRRKTTKVVWNDHFTLAQSPTFTSSADNVVVKSITFVNSFNF 76
           +R  V IP +K +I L+G+  + T + ++DH     S TFT+ AD++V+  ITF N++N 
Sbjct: 72  YREKVTIPKEKGFIYLQGKGIEQTVIEYDDHQATDISATFTAFADDIVISGITFKNTYNI 131

Query: 77  PWTNGNRRAPAVAAMITGDKALFYKCGFYGVQDTLWDDQGRHYYHRCVIQVVGEALLPLG 136
              N     PAVAA + GD+ +     F G+QDTL+D +GRHYY RC+I    + +   G
Sbjct: 132 VPNNKREIVPAVAARMLGDRYVVTDSSFVGLQDTLFDGKGRHYYKRCIISGGIDFIFGYG 191

Query: 137 STSF--------------------ITAQGRTDPNDANGFVFKDCKVFGSGSAFLGRPWRG 196
            + F                    ITA  R  P+D  GFVF DC V G G   LGR W  
Sbjct: 192 QSLFKECTLNMTLGIYAPDNPYGTITAHQRPSPSDEGGFVFSDCTVTGVGKTLLGRAWGS 251

Query: 197 YSRVIFYNSSFSNVINPSGWDPWHFLLSIFKILGDQ--LTYAEYGCYGPGSDMSHRVKWE 256
            +RVIF  S  S+V+ P GWD W       +  G++  LT+ E GC G G+D S RV W 
Sbjct: 252 NARVIFDRSRLSDVVLPIGWDAW-------RAKGNERDLTFVEAGCTGAGADTSQRVPWL 311

Query: 257 KKLDLDEIQQLTDMSFIDTEGWMQDQP 262
           KKL L E+     +SFID +GW+   P
Sbjct: 312 KKLSLSEVDGFASVSFIDQDGWISRFP 331

[Close]
BLAST of Cp4.1LG12g00960 vs. TAIR10
Match: AT2G21610.1 (AT2G21610.1 pectinesterase 11)

HSP 1 Score: 184.5 bits (467), Expect = 8.4e-47
Identity = 101/264 (38.26%), Postives = 133/264 (50.38%), Query Frame = 1 

Query: 17  WRTVVKIPYDKPYIILKGQRRKTTKVVWNDHFTLAQSPTFTSSADNVVVKSITFVNSFNF 76
           +R  V IP +KPYI L G +   T ++W+D   + +SPT T  A + V + +T  N F  
Sbjct: 90  YREKVVIPAEKPYITLSGTQASNTFLIWSDGEDILESPTLTIFASDFVCRFLTIQNKFG- 149

Query: 77  PWTNGNRRAPAVAAMITGDKALFYKCGFYGVQDTLWDDQGRHYYHRCVIQVVGEALLPLG 136
             T G     AVA  +  DKA FY C     QDTL DD G HY+  C I+   + +    
Sbjct: 150 --TAGR----AVALRVAADKAAFYGCVITSYQDTLLDDNGNHYFKNCYIEGATDFICGSA 209

Query: 137 STSF--------------ITAQGRTDPNDANGFVFKDCKVFGSGSAFLGRPWRGYSRVIF 196
           S+ +              ITAQ RT   + +GF F  CK+ GSGS FLGRPW  YSRV+F
Sbjct: 210 SSLYERCHLHSLSPNNGSITAQMRTSATEKSGFTFLGCKLTGSGSTFLGRPWGAYSRVVF 269

Query: 197 YNSSFSNVINPSGWDPWHFLLSIFKILGDQ-----LTYAEYGCYGPGSDMSHRVKWEKKL 256
             S FSNV+ P GW+ W          GD      + Y EY CYGPG+D   RV+W K+L
Sbjct: 270 AYSFFSNVVAPQGWNQW----------GDSTKENTVYYGEYKCYGPGADREQRVEWSKQL 329

Query: 257 DLDEIQQLTDMSFIDTEGWMQDQP 262
             +E        FI  + W++  P
Sbjct: 330 SDEEATVFLSKDFIGGKDWLRPAP 336

[Close]
BLAST of Cp4.1LG12g00960 vs. NCBI nr
Match: gi|659114888|ref|XP_008457276.1| (PREDICTED: probable pectinesterase 29 [Cucumis melo])

HSP 1 Score: 423.7 bits (1088), Expect = 2.3e-115
Identity = 204/322 (63.35%), Postives = 230/322 (71.43%), Query Frame = 1 

Query: 1   GNEDERYRSVGGKKLAWRTV---------------------------------------- 60
           GNE E YR +G KK AW+T+                                        
Sbjct: 19  GNEGESYRRIGRKKFAWKTLIVDKNGHGNFSTIQAAIDSVPSDNRFWVSIHIRPGLYREK 78

Query: 61  VKIPYDKPYIILKGQRRKTTKVVWNDHFTLAQSPTFTSSADNVVVKSITFVNSFNFPWTN 120
           VKIPYDKPYIILKG R++ TKVVW+DH T+AQSPTFTSSADN+VVKSI+FVNS+N+PW N
Sbjct: 79  VKIPYDKPYIILKGHRKRNTKVVWDDHLTVAQSPTFTSSADNIVVKSISFVNSYNYPWKN 138

Query: 121 GNRRAPAVAAMITGDKALFYKCGFYGVQDTLWDDQGRHYYHRCVIQ-------------- 180
           GN R PAVAAMITGDK+ FY+CGFYGVQDTLWD+QGRHYYHRC IQ              
Sbjct: 139 GNPRVPAVAAMITGDKSSFYRCGFYGVQDTLWDNQGRHYYHRCTIQGAVDFIFGAAQSIF 198

Query: 181 ------VVGEALLPLGSTSFITAQGRTDPNDANGFVFKDCKVFGSGSAFLGRPWRGYSRV 240
                 VVGEALLPLGSTSFITAQGRTDPNDANGFVFK+C VFGSGSA+LGRPWR YSRV
Sbjct: 199 QGCSISVVGEALLPLGSTSFITAQGRTDPNDANGFVFKECNVFGSGSAYLGRPWRAYSRV 258

Query: 241 IFYNSSFSNVINPSGWDPWHFLLSIFKILGDQLTYAEYGCYGPGSDMSHRVKWEKKLDLD 263
           IF+NS+FSN+INP+GWDPWHF+        +QLTYAE  CYGPGSD+S RV WEKKL   
Sbjct: 259 IFHNSNFSNIINPNGWDPWHFV-----GYENQLTYAENDCYGPGSDISRRVSWEKKLSPK 318

[Close]
BLAST of Cp4.1LG12g00960 vs. NCBI nr
Match: gi|778674132|ref|XP_011650140.1| (PREDICTED: probable pectinesterase 29 [Cucumis sativus])

HSP 1 Score: 409.1 bits (1050), Expect = 6.0e-111
Identity = 198/322 (61.49%), Postives = 225/322 (69.88%), Query Frame = 1 

Query: 1   GNEDERYRSVGGKKLAWRTV---------------------------------------- 60
           GNE E YR VG KK AW+T+                                        
Sbjct: 20  GNEGEPYRRVGSKKFAWKTLIVDKKGHGNFSTIQAAIDSVPSNNRFWVSIHIRPGLYREK 79

Query: 61  VKIPYDKPYIILKGQRRKTTKVVWNDHFTLAQSPTFTSSADNVVVKSITFVNSFNFPWTN 120
           VKIPYDKPYIILKG R++ TKVVW+DH T+AQSPTFTSSADN+VVKSI+FVNS+N+PW N
Sbjct: 80  VKIPYDKPYIILKGHRKRRTKVVWDDHLTVAQSPTFTSSADNIVVKSISFVNSYNYPWKN 139

Query: 121 GNRRAPAVAAMITGDKALFYKCGFYGVQDTLWDDQGRHYYHRCVIQ-------------- 180
           GN R PAVAAMITGDK+ FY+CGFYGVQDTLWD+QGRHYYHRC IQ              
Sbjct: 140 GNPRVPAVAAMITGDKSSFYRCGFYGVQDTLWDNQGRHYYHRCTIQGAVDFIFGAAQSIF 199

Query: 181 ------VVGEALLPLGSTSFITAQGRTDPNDANGFVFKDCKVFGSGSAFLGRPWRGYSRV 240
                 VVGEALLP GSTSFITAQGRT+PNDANGFVFK+C VFGSGSA+LGRPWR YSRV
Sbjct: 200 QGCSISVVGEALLPYGSTSFITAQGRTNPNDANGFVFKECNVFGSGSAYLGRPWRAYSRV 259

Query: 241 IFYNSSFSNVINPSGWDPWHFLLSIFKILGDQLTYAEYGCYGPGSDMSHRVKWEKKLDLD 263
           IF+NS+FSN+INP+GWDPW F+        + LTY E  CYGPGSD+S RV WEKKL   
Sbjct: 260 IFHNSNFSNIINPNGWDPWQFV-----GYENHLTYVENDCYGPGSDISGRVSWEKKLSWK 319

[Close]
BLAST of Cp4.1LG12g00960 vs. NCBI nr
Match: gi|703142522|ref|XP_010107773.1| (putative pectinesterase 29 [Morus notabilis])

HSP 1 Score: 338.2 bits (866), Expect = 1.3e-89
Identity = 164/266 (61.65%), Postives = 194/266 (72.93%), Query Frame = 1 

Query: 17  WRTVVKIPYDKPYIILKGQRRKTTKVVWNDHFTLAQSPTFTSSADNVVVKSITFVNSFNF 76
           +R  +KIPYDKPYIILKG+ R+ T+VVW+DH TLAQSPTFTS ADN+V K ++F NS+N 
Sbjct: 63  YREKIKIPYDKPYIILKGEGRRKTQVVWDDHDTLAQSPTFTSLADNIVAKCMSFTNSYNR 122

Query: 77  PWTNGNRRAPAVAAMITGDKALFYKCGFYGVQDTLWDDQGRHYY---------------- 136
           P  N   RAPA AAMITGDK  F++CGF+G+QDTLWDDQGRHYY                
Sbjct: 123 P-PNDKPRAPAAAAMITGDKTKFFRCGFFGLQDTLWDDQGRHYYKLCTIQGAVDFIFGSG 182

Query: 137 ----HRCVIQVVGEALLPLGSTSFITAQGRTDPNDANGFVFKDCKVFGSGSAFLGRPWRG 196
                RC I VVG AL P G   FITAQGRT+PNDANGFVFKDC VFGSGSAFLGRPWRG
Sbjct: 183 QSIFERCSISVVGGALEP-GFQGFITAQGRTNPNDANGFVFKDCHVFGSGSAFLGRPWRG 242

Query: 197 YSRVIFYNSSFSNVINPSGWDPWHFLLSIFKILGDQLTYAEYGCYGPGSDMSHRVKWEKK 256
           Y+RV FYN++ S+VI P GW+ W+F    +     QLTYAE GC+GPGSD S RV WEKK
Sbjct: 243 YARVFFYNTNISDVIVPQGWNAWNFAGQEY-----QLTYAEEGCFGPGSDSSRRVSWEKK 302

Query: 257 LDLDEIQQLTDMSFIDTEGWMQDQPW 263
           L+ + ++QLT  SFID EGW+ +QP+
Sbjct: 303 LNEETVKQLTSSSFIDDEGWLNNQPF 321

[Close]
BLAST of Cp4.1LG12g00960 vs. NCBI nr
Match: gi|596263779|ref|XP_007224981.1| (hypothetical protein PRUPE_ppa020682mg [Prunus persica])

HSP 1 Score: 336.3 bits (861), Expect = 4.9e-89
Identity = 160/274 (58.39%), Postives = 199/274 (72.63%), Query Frame = 1 

Query: 9   SVGGKKLAWRTVVKIPYDKPYIILKGQRRKTTKVVWNDHFTLAQSPTFTSSADNVVVKSI 68
           S+  K   ++  V IP DKPYIILKG+ R  T++VW+DH ++AQSPTF S AD+++VKSI
Sbjct: 45  SIKIKAGTYKEKVIIPVDKPYIILKGENRHKTQIVWDDHDSVAQSPTFASYADSIIVKSI 104

Query: 69  TFVNSFNFPWTNGNRRAPAVAAMITGDKALFYKCGFYGVQDTLWDDQGRHYYH------- 128
           +FVNS+N P  N N R  AVAAMI GDK+ FY+CGF+G+QDTLWD QGRHYYH       
Sbjct: 105 SFVNSYNNPVNNKNPRVAAVAAMIYGDKSSFYRCGFFGLQDTLWDGQGRHYYHLCTIQGA 164

Query: 129 -------------RCVIQVVGEALLPLGSTSFITAQGRTDPNDANGFVFKDCKVFGSGSA 188
                        +C IQV+G AL P GS  +ITAQGR +PNDA+GFVFKDCKV G+GS 
Sbjct: 165 VDFIFGSAQSIFQKCSIQVLGGALDP-GSAGYITAQGRDNPNDASGFVFKDCKVSGTGST 224

Query: 189 FLGRPWRGYSRVIFYNSSFSNVINPSGWDPWHFLLSIFKILGDQLTYAEYGCYGPGSDMS 248
           FLGR WRGYSRVIFYNS+FS V+ P GWD WHF  +       QLTYAE+GCYGPG+D  
Sbjct: 225 FLGRAWRGYSRVIFYNSNFSQVVVPQGWDAWHFQGN-----EHQLTYAEHGCYGPGADTV 284

Query: 249 HRVKWEKKLDLDEIQQLTDMSFIDTEGWMQDQPW 263
            RV+WEKKL+ D +++LT ++FIDT+GW+ DQP+
Sbjct: 285 KRVEWEKKLNADTVRELTSLNFIDTDGWLNDQPF 312

[Close]
BLAST of Cp4.1LG12g00960 vs. NCBI nr
Match: gi|645236029|ref|XP_008224542.1| (PREDICTED: probable pectinesterase 29 [Prunus mume])

HSP 1 Score: 333.6 bits (854), Expect = 3.2e-88
Identity = 158/274 (57.66%), Postives = 198/274 (72.26%), Query Frame = 1 

Query: 9   SVGGKKLAWRTVVKIPYDKPYIILKGQRRKTTKVVWNDHFTLAQSPTFTSSADNVVVKSI 68
           S+  K   ++  V IP DKPYIILKG+ R  T +VW+DH ++AQSPTF S AD+++VKSI
Sbjct: 77  SIKIKAGTYKEKVIIPIDKPYIILKGENRHKTLIVWDDHDSVAQSPTFASYADSIIVKSI 136

Query: 69  TFVNSFNFPWTNGNRRAPAVAAMITGDKALFYKCGFYGVQDTLWDDQGRHYYH------- 128
           +FVNS+N P  N N R  AVAAMI GDK+ FY+CGF+G+QDTLWD QGRHYYH       
Sbjct: 137 SFVNSYNNPVNNKNPRVAAVAAMIYGDKSSFYRCGFFGLQDTLWDGQGRHYYHLCTIQGA 196

Query: 129 -------------RCVIQVVGEALLPLGSTSFITAQGRTDPNDANGFVFKDCKVFGSGSA 188
                        +C IQV+G AL P GS  +ITAQGR +PNDA+GFVFKDCKV G+GS 
Sbjct: 197 VDFIFGSAQSIFEKCSIQVLGGALDP-GSAGYITAQGRDNPNDASGFVFKDCKVSGTGST 256

Query: 189 FLGRPWRGYSRVIFYNSSFSNVINPSGWDPWHFLLSIFKILGDQLTYAEYGCYGPGSDMS 248
           +LGR WRGYSRVIFYNS+FS ++ P GWD WHF  +       QLTYAE+GCYGPG+D  
Sbjct: 257 YLGRAWRGYSRVIFYNSNFSKIVVPQGWDAWHFQGN-----EHQLTYAEHGCYGPGADTV 316

Query: 249 HRVKWEKKLDLDEIQQLTDMSFIDTEGWMQDQPW 263
            RV+WEKKL+ D +++LT ++FIDT+GW+ DQP+
Sbjct: 317 KRVEWEKKLNADTVRELTSLNFIDTDGWLNDQPF 344

The following BLAST results are available for this feature:
BLAST of Cp4.1LG12g00960 vs. Swiss-Prot
Match NameE-valueIdentityDescription
PME29_ARATH3.2e-8053.36Probable pectinesterase 29 OS=Arabidopsis thaliana GN=PME29 PE=2 SV=1[more]
PME55_ARATH3.1e-7551.87Probable pectinesterase 55 OS=Arabidopsis thaliana GN=PME55 PE=2 SV=1[more]
PME10_ARATH6.3e-5242.21Putative pectinesterase 10 OS=Arabidopsis thaliana GN=PME10 PE=2 SV=1[more]
PME66_ARATH1.9e-4838.95Probable pectinesterase 66 OS=Arabidopsis thaliana GN=PME66 PE=2 SV=2[more]
PME11_ARATH1.5e-4538.26Putative pectinesterase 11 OS=Arabidopsis thaliana GN=PME11 PE=3 SV=1[more]
BLAST of Cp4.1LG12g00960 vs. TrEMBL
Match NameE-valueIdentityDescription
W9SM62_9ROSA9.0e-9061.65Pectinesterase OS=Morus notabilis GN=L484_021593 PE=4 SV=1[more]
M5Y357_PRUPE3.4e-8958.39Pectinesterase OS=Prunus persica GN=PRUPE_ppa020682mg PE=4 SV=1[more]
A0A061ELR2_THECC6.0e-8657.74Pectinesterase OS=Theobroma cacao GN=TCM_020334 PE=4 SV=1[more]
B9RWI7_RICCO8.7e-8558.37Pectinesterase OS=Ricinus communis GN=RCOM_1019830 PE=4 SV=1[more]
A0A059CS97_EUCGR4.3e-8456.13Pectinesterase (Fragment) OS=Eucalyptus grandis GN=EUGRSUZ_C02449 PE=4 SV=1[more]
BLAST of Cp4.1LG12g00960 vs. TAIR10
Match NameE-valueIdentityDescription
AT3G24130.11.8e-8153.36 Pectin lyase-like superfamily protein[more]
AT5G18990.11.7e-7651.87 Pectin lyase-like superfamily protein[more]
AT2G19150.13.5e-5342.21 Pectin lyase-like superfamily protein[more]
AT2G47280.11.1e-4938.95 Pectin lyase-like superfamily protein[more]
AT2G21610.18.4e-4738.26 pectinesterase 11[more]
BLAST of Cp4.1LG12g00960 vs. NCBI nr
Match NameE-valueIdentityDescription
gi|659114888|ref|XP_008457276.1|2.3e-11563.35PREDICTED: probable pectinesterase 29 [Cucumis melo][more]
gi|778674132|ref|XP_011650140.1|6.0e-11161.49PREDICTED: probable pectinesterase 29 [Cucumis sativus][more]
gi|703142522|ref|XP_010107773.1|1.3e-8961.65putative pectinesterase 29 [Morus notabilis][more]
gi|596263779|ref|XP_007224981.1|4.9e-8958.39hypothetical protein PRUPE_ppa020682mg [Prunus persica][more]
gi|645236029|ref|XP_008224542.1|3.2e-8857.66PREDICTED: probable pectinesterase 29 [Prunus mume][more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: Biological Process
TermDefinition
GO:0042545cell wall modification
Vocabulary: Molecular Function
TermDefinition
GO:0030599pectinesterase activity
Vocabulary: Cellular Component
TermDefinition
GO:0005618cell wall
Vocabulary: INTERPRO
TermDefinition
IPR012334Pectin_lyas_fold
IPR011050Pectin_lyase_fold/virulence
IPR000070Pectinesterase_cat
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0042545 cell wall modification
biological_process GO:0005982 starch metabolic process
biological_process GO:0005985 sucrose metabolic process
biological_process GO:0045490 pectin catabolic process
cellular_component GO:0005618 cell wall
cellular_component GO:0005576 extracellular region
molecular_function GO:0030599 pectinesterase activity
molecular_function GO:0045330 aspartyl esterase activity
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG12g00960.1Cp4.1LG12g00960.1mRNA


InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo
Date Performed: 2017-12-02
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000070Pectinesterase, catalyticPFAMPF01095Pectinesterasecoord: 17..126
score: 6.3E-14coord: 138..253
score: 7.7
IPR011050Pectin lyase fold/virulence factorunknownSSF51126Pectin lyase-likecoord: 20..258
score: 4.44
IPR012334Pectin lyase foldGENE3DG3DSA:2.160.20.10coord: 20..259
score: 2.8
NoneNo IPR availablePANTHERPTHR31321FAMILY NOT NAMEDcoord: 21..261
score: 2.0E
NoneNo IPR availablePANTHERPTHR31321:SF11PECTINESTERASE 10-RELATEDcoord: 21..261
score: 2.0E

Synteny
The following gene(s) are orthologous to this gene:
GeneOrthologueOrganismBlock
Cp4.1LG12g00960Cucsa.193880Cucumber (Gy14) v1cgycpeB0546
Cp4.1LG12g00960CmaCh17G001040Cucurbita maxima (Rimu)cmacpeB371
Cp4.1LG12g00960CmoCh17G001080Cucurbita moschata (Rifu)cmocpeB335
Cp4.1LG12g00960Cla002954Watermelon (97103) v1cpewmB161
Cp4.1LG12g00960Csa2G138720Cucumber (Chinese Long) v2cpecuB131
Cp4.1LG12g00960MELO3C020451Melon (DHL92) v3.5.1cpemeB137
Cp4.1LG12g00960ClCG06G007740Watermelon (Charleston Gray)cpewcgB144
Cp4.1LG12g00960CSPI02G09100Wild cucumber (PI 183967)cpecpiB132
Cp4.1LG12g00960Lsi09G011490Bottle gourd (USVL1VR-Ls)cpelsiB101
Cp4.1LG12g00960MELO3C020451.2Melon (DHL92) v3.6.1cpemedB166
Cp4.1LG12g00960CsaV3_2G011500Cucumber (Chinese Long) v3cpecucB0151
Cp4.1LG12g00960Bhi12G001302Wax gourdcpewgoB0176
Cp4.1LG12g00960CsGy2G009350Cucumber (Gy14) v2cgybcpeB180
Cp4.1LG12g00960Carg10139Silver-seed gourdcarcpeB0322
The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:
GeneOrganismBlock
Cp4.1LG12g00960Cucurbita pepo (Zucchini)cpecpeB169
Cp4.1LG12g00960Cucurbita pepo (Zucchini)cpecpeB184
Cp4.1LG12g00960Cucurbita pepo (Zucchini)cpecpeB189
Cp4.1LG12g00960Cucurbita pepo (Zucchini)cpecpeB194
Cp4.1LG12g00960Cucumber (Gy14) v1cgycpeB0670
Cp4.1LG12g00960Cucumber (Gy14) v1cgycpeB0698
Cp4.1LG12g00960Cucurbita maxima (Rimu)cmacpeB259
Cp4.1LG12g00960Cucurbita maxima (Rimu)cmacpeB519
Cp4.1LG12g00960Cucurbita maxima (Rimu)cmacpeB600
Cp4.1LG12g00960Cucurbita maxima (Rimu)cmacpeB895
Cp4.1LG12g00960Cucurbita moschata (Rifu)cmocpeB223
Cp4.1LG12g00960Cucurbita moschata (Rifu)cmocpeB549
Cp4.1LG12g00960Cucurbita moschata (Rifu)cmocpeB765
Cp4.1LG12g00960Cucurbita moschata (Rifu)cmocpeB833
Cp4.1LG12g00960Wild cucumber (PI 183967)cpecpiB127
Cp4.1LG12g00960Cucumber (Chinese Long) v2cpecuB128
Cp4.1LG12g00960Watermelon (Charleston Gray)cpewcgB138
Cp4.1LG12g00960Watermelon (97103) v1cpewmB151
Cp4.1LG12g00960Melon (DHL92) v3.5.1cpemeB141
Cp4.1LG12g00960Cucumber (Gy14) v2cgybcpeB175
Cp4.1LG12g00960Cucumber (Gy14) v2cgybcpeB176
Cp4.1LG12g00960Melon (DHL92) v3.6.1cpemedB170
Cp4.1LG12g00960Silver-seed gourdcarcpeB1087
Cp4.1LG12g00960Silver-seed gourdcarcpeB1328
Cp4.1LG12g00960Silver-seed gourdcarcpeB1501
Cp4.1LG12g00960Cucumber (Chinese Long) v3cpecucB0160
Cp4.1LG12g00960Cucumber (Chinese Long) v3cpecucB0146
Cp4.1LG12g00960Cucumber (Chinese Long) v3cpecucB0153
Cp4.1LG12g00960Wax gourdcpewgoB0192