Cp4.1LG03g15960 (gene) Cucurbita pepo (Zucchini)

Overview
NameCp4.1LG03g15960
Typegene
OrganismCucurbita pepo (Cucurbita pepo (Zucchini))
DescriptionPyridoxamine 5'-phosphate oxidase family protein
LocationCp4.1LG03 : 13499032 .. 13500934 (+)
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CACGACAAAACAAAGATGGGCAAGAATACACGTAATTCCACGCCGACCAATCCAATAAAAATGATTTAATGTATTTGTTCATTTTAATAATTTAGTGTATGGATAAAAGATTGTGGTAAAATTGAAAGCTAAAAGTAAATTACTGAATTTCGCTTACTATTTCTCAATGATGTACCGAACTGAAATGGGGCGAATACATAGCAGAGCAGTAGGCGAATTAGCGGGCAATTAGGAAATGGGATCGGCAGCGGCGCCATGGAAGTCCCTTCTTCTCAGTGCTTTGGAATCAAATGCCCAAATCAAGCATTCCAGATTCTTGCACCTCGTACATTTCCCTCTCTCTATTTCTCTTCAACTTCATCTTCAACTCCCCTTTCCCTTTTTCACACTCGGGTTTTCTTTTTATAAATGGAATTTGTTTTCAGGCAACGATTGGAGGCAATGGAAGGCCATCAAATCGCACCGTTGTTTTCAGAGGATTTGAAGAGGGCACCGATGGAATCCATATTTACACGGATTCAAGAAGTCGCAAGGTTCTTCTACGACGAAGCTCTATATTTCGTTTTAGATCGCTCAATTCATACGCTCATTTCTTGTTGTTCTATGGTTTCCGACGCAATCATTATCTGGGTTTCTGGATTTTCTGCATTTTCTGCATTTTCAATTATGTAATTCATATGTTTGGCTACAATAAGATAAGCTATAAAATATGCTCAGTACTGGGAATCTGTCTGCGATGGATTAGGTCAGGTTTGTGATATTAAGCATCTGAGTTTTGTCCTGCAAATGAACTTTTCACTTTTGGTTGATTCTGCAGATTGAAGAGCTTAAAGCTTGTCCATTTGCTGAGGTAACTATTCTCGTCTCTTCCTGCCTCTTAATACAGTTCATAATACAAATGATTTGTTCTTGCTGGTCTATGTAGCTTATCTTATTTACATAACTGCAAATATGTGCCAATTTGAGTCAAATTTTGGACTTCGAATGTTTTGTTCTTCAAGATAAGTTGGTATTTCACCGAATCATGGGAGCAATTTCGTATCAGTGGAAGAATTGAGGTTGTTGATGCGTCATGTCTTGATGCTACAAAATTTAAGGTTGTGGGCATGACCCTTTCCTCTTATAACTTAGAACTTGTTCTAAATTCTCAACAGTTCACTTTCTTGTTCACTTCATTCAACTCAGAATAATCTTATATATTTATTAATGCAGAAAAGAGCAGAAGCTTGGTCATCCATTTCTCTAAAGTCAAGAATGCAGTATTTGGGGCCTAGTCCAAATCTTCCCTATATTGCCGAACAGCCAGCCAAGGAACCTGTTCTAGATCCTTGTTCAGGTCCTGTTGATGCATTTTGTTTGCTGGTCTTGGATCCTGATCAGGTTTGTAATATATATAGACATATTTCTGTAGATTAGTATATGACCTTGTTCTTTTTCGTTATGTTACATTTGACCGCGTTTCGCTCTCAGGTCGATTATTTGAATCTGAAGAGTGAAGAAAGGAAGTTGTTTAAAGCTATTCCATGTCTTGGAAGGGAGACATTATGGGAATCAGAGCGGATAAACCCTTAGCAATTGCACCTCGATTTGCACAGGTATATTGATAACAACAAGCTGGTATCTTGAAATGAACTGATATAGTTAGTTACTCATGTGTTGAAAATAATCCTCCGACCCTTTGGTTGTTAGTAGAGATGATACTGATTGAACAATCATGGTTCCCAAGTTATGAGGGGTTTTGTAGCTTCAAATTCTTTTTGCTCTACTGCCATGGCTATATCTGTAGATTGGGTGTATGTAACTTAATTCTCATGCTCTTCAACCTGAAACAAAAGGAATGAAAAGTTGGAATGAGTTGTTTGAAGCATGAAATATGCAAAGAAACAGAAGGATAGGAAAGGCT

mRNA sequence

CACGACAAAACAAAGATGGGCAAGAATACACGTAATTCCACGCCGACCAATCCAATAAAAATGATTTAATGTATTTGTTCATTTTAATAATTTAGTGTATGGATAAAAGATTGTGGTAAAATTGAAAGCTAAAAGTAAATTACTGAATTTCGCTTACTATTTCTCAATGATGTACCGAACTGAAATGGGGCGAATACATAGCAGAGCAGTAGGCGAATTAGCGGGCAATTAGGAAATGGGATCGGCAGCGGCGCCATGGAAGTCCCTTCTTCTCAGTGCTTTGGAATCAAATGCCCAAATCAAGCATTCCAGATTCTTGCACCTCGCAACGATTGGAGGCAATGGAAGGCCATCAAATCGCACCGTTGTTTTCAGAGGATTTGAAGAGGGCACCGATGGAATCCATATTTACACGGATTCAAGAAGTCGCAAGATTGAAGAGCTTAAAGCTTGTCCATTTGCTGAGATAAGTTGGTATTTCACCGAATCATGGGAGCAATTTCGTATCAGTGGAAGAATTGAGGTTGTTGATGCGTCATGTCTTGATGCTACAAAATTTAAGAAAAGAGCAGAAGCTTGGTCATCCATTTCTCTAAAGTCAAGAATGCAGTATTTGGGGCCTAGTCCAAATCTTCCCTATATTGCCGAACAGCCAGCCAAGGAACCTGTTCTAGATCCTTGTTCAGGTCCTGTTGATGCATTTTGTTTGCTGGTCTTGGATCCTGATCAGGTCGATTATTTGAATCTGAAGAGTGAAGAAAGGAAGTTGTTTAAAGCTATTCCATGTCTTGGAAGGGAGACATTATGGGAATCAGAGCGGATAAACCCTTAGCAATTGCACCTCGATTTGCACAGGTATATTGATAACAACAAGCTGGTATCTTGAAATGAACTGATATAGTTAGTTACTCATGTGTTGAAAATAATCCTCCGACCCTTTGGTTGTTAGTAGAGATGATACTGATTGAACAATCATGGTTCCCAAGTTATGAGGGGTTTTGTAGCTTCAAATTCTTTTTGCTCTACTGCCATGGCTATATCTGTAGATTGGGTGTATGTAACTTAATTCTCATGCTCTTCAACCTGAAACAAAAGGAATGAAAAGTTGGAATGAGTTGTTTGAAGCATGAAATATGCAAAGAAACAGAAGGATAGGAAAGGCT

Coding sequence (CDS)

ATGGGATCGGCAGCGGCGCCATGGAAGTCCCTTCTTCTCAGTGCTTTGGAATCAAATGCCCAAATCAAGCATTCCAGATTCTTGCACCTCGCAACGATTGGAGGCAATGGAAGGCCATCAAATCGCACCGTTGTTTTCAGAGGATTTGAAGAGGGCACCGATGGAATCCATATTTACACGGATTCAAGAAGTCGCAAGATTGAAGAGCTTAAAGCTTGTCCATTTGCTGAGATAAGTTGGTATTTCACCGAATCATGGGAGCAATTTCGTATCAGTGGAAGAATTGAGGTTGTTGATGCGTCATGTCTTGATGCTACAAAATTTAAGAAAAGAGCAGAAGCTTGGTCATCCATTTCTCTAAAGTCAAGAATGCAGTATTTGGGGCCTAGTCCAAATCTTCCCTATATTGCCGAACAGCCAGCCAAGGAACCTGTTCTAGATCCTTGTTCAGGTCCTGTTGATGCATTTTGTTTGCTGGTCTTGGATCCTGATCAGGTCGATTATTTGAATCTGAAGAGTGAAGAAAGGAAGTTGTTTAAAGCTATTCCATGTCTTGGAAGGGAGACATTATGGGAATCAGAGCGGATAAACCCTTAG

Protein sequence

MGSAAAPWKSLLLSALESNAQIKHSRFLHLATIGGNGRPSNRTVVFRGFEEGTDGIHIYTDSRSRKIEELKACPFAEISWYFTESWEQFRISGRIEVVDASCLDATKFKKRAEAWSSISLKSRMQYLGPSPNLPYIAEQPAKEPVLDPCSGPVDAFCLLVLDPDQVDYLNLKSEERKLFKAIPCLGRETLWESERINP
Homology
[Close]
BLAST of Cp4.1LG03g15960 vs. Swiss-Prot
Match: PPOX2_ARATH (Pyridoxine/pyridoxamine 5'-phosphate oxidase 2 OS=Arabidopsis thaliana GN=PPOX2 PE=1 SV=2)

HSP 1 Score: 240.7 bits (613), Expect = 1.3e-62
Identity = 112/198 (56.57%), Postives = 150/198 (75.76%), Query Frame = 1 

Query: 1   MGSAAAPWKSLLLSALESNAQIKHSRFLHLATIGGNGRPSNRTVVFRGFEEGTDGIHIYT 60
           MG+  APWK LL  A+E+N+ + HS ++ LATIG NGRPSNRTVVFRGFEE +D I I T
Sbjct: 1   MGTHVAPWKQLLFGAIEANSHLSHSSYVQLATIGLNGRPSNRTVVFRGFEENSDRIQINT 60

Query: 61  DSRSRKIEELKACPFAEISWYFTESWEQFRISGRIEVVDASCLDATKFKKRAEAWSSISL 120
           D RSRKIEELK CPF+E+ WYF+++WEQFRI+GRIEV+DAS  D TK ++R +AW + SL
Sbjct: 61  DLRSRKIEELKHCPFSEMCWYFSDTWEQFRINGRIEVIDASNPDQTKLQQREKAWFANSL 120

Query: 121 KSRMQYLGPSPNLPYIAEQPAKEPVLDPCSGPVDAFCLLVLDPDQVDYLNLKSEERKLFK 180
           +SR+ Y+ P+P  P  +EQ +++  LDP SGPV  +CLL+L+P++VDYLNLK+ +R  F 
Sbjct: 121 RSRLIYVCPTPGSPCNSEQSSQQVKLDPSSGPVPEYCLLLLEPEKVDYLNLKTNQRLFFS 180

Query: 181 AIPCLGRETLWESERINP 199
           ++     E  W SE++NP
Sbjct: 181 SMATGTGEKCWTSEKVNP 198

[Close]
BLAST of Cp4.1LG03g15960 vs. Swiss-Prot
Match: PDXH_MYXXA (Pyridoxine/pyridoxamine 5'-phosphate oxidase OS=Myxococcus xanthus GN=pdxH PE=1 SV=2)

HSP 1 Score: 62.4 bits (150), Expect = 6.5e-09
Identity = 50/189 (26.46%), Postives = 91/189 (48.15%), Query Frame = 1 

Query: 15  ALESNAQIKHSRFLHLATIGGNGRPSNRTVVFRGFEEGTDGIHIYTDSRSRKIEELKACP 74
           A++ NA +       +AT+G +GRPS R V+ + F+    G   YT+  SRK  E +A P
Sbjct: 49  AVDPNAMV-------VATVGDDGRPSARVVLLKDFD--ARGFVFYTNHESRKGREARAHP 108

Query: 75  FAEISWYFTESWEQFRISGRIEVVDASCLDATKFKKRAE-----AWSSISLKSRMQYLGP 134
           +A + +Y+    EQ R+ GR+E V  +  DA  F+ RA      AW+S+  +        
Sbjct: 109 YAALCFYWQPLNEQVRVEGRVERVTDAEADA-YFQSRARGSQVGAWASLQSQPLATREEL 168

Query: 135 SPNLPYIAEQPAKEPVLDPCSGPVDAFCLLVLDPDQVDYLNLKSEERKLFKAIPCLGRET 194
              +  + ++ A +PV  P       +    + PD++++ +  ++E +L      L  + 
Sbjct: 169 EARVAEVEQKYAGQPVPRP-----PHWSGFRVVPDRIEFWH--AQESRLHDRHVYLREDG 220

Query: 195 LWESERINP 199
            W ++ + P
Sbjct: 229 GWRTQMLYP 220

[Close]
BLAST of Cp4.1LG03g15960 vs. Swiss-Prot
Match: PDXH_MYXXD (Pyridoxine/pyridoxamine 5'-phosphate oxidase OS=Myxococcus xanthus (strain DK 1622) GN=pdxH PE=3 SV=2)

HSP 1 Score: 62.4 bits (150), Expect = 6.5e-09
Identity = 50/189 (26.46%), Postives = 91/189 (48.15%), Query Frame = 1 

Query: 15  ALESNAQIKHSRFLHLATIGGNGRPSNRTVVFRGFEEGTDGIHIYTDSRSRKIEELKACP 74
           A++ NA +       +AT+G +GRPS R V+ + F+    G   YT+  SRK  E +A P
Sbjct: 35  AVDPNAMV-------VATVGDDGRPSARVVLLKDFD--ARGFVFYTNHESRKGREARAHP 94

Query: 75  FAEISWYFTESWEQFRISGRIEVVDASCLDATKFKKRAE-----AWSSISLKSRMQYLGP 134
           +A + +Y+    EQ R+ GR+E V  +  DA  F+ RA      AW+S+  +        
Sbjct: 95  YAALCFYWQPLNEQVRVEGRVERVTDAEADA-YFQSRARGSQVGAWASLQSQPLATREEL 154

Query: 135 SPNLPYIAEQPAKEPVLDPCSGPVDAFCLLVLDPDQVDYLNLKSEERKLFKAIPCLGRET 194
              +  + ++ A +PV  P       +    + PD++++ +  ++E +L      L  + 
Sbjct: 155 EARVAEVEQKYAGQPVPRP-----PHWSGFRVVPDRIEFWH--AQESRLHDRHVYLREDG 206

Query: 195 LWESERINP 199
            W ++ + P
Sbjct: 215 GWRTQMLYP 206

[Close]
BLAST of Cp4.1LG03g15960 vs. Swiss-Prot
Match: PDXH_CHRVO (Pyridoxine/pyridoxamine 5'-phosphate oxidase OS=Chromobacterium violaceum (strain ATCC 12472 / DSM 30191 / JCM 1249 / NBRC 12614 / NCIMB 9131 / NCTC 9757) GN=pdxH PE=3 SV=1)

HSP 1 Score: 61.6 bits (148), Expect = 1.1e-08
Identity = 35/102 (34.31%), Postives = 57/102 (55.88%), Query Frame = 1 

Query: 20  AQIKHSRFLHLATIGGNGRPSNRTVVFRGFEEGTDGIHIYTDSRSRKIEELKACPFAEIS 79
           AQ+     ++LA IG +GRPS+R V+ +G E+G   +  YT+ +SRK + L+A P+  ++
Sbjct: 40  AQVPEPTAMNLAAIGADGRPSSRIVLLKGVEDGQ--LLFYTNYQSRKGQALEANPYVALN 99

Query: 80  WYFTESWEQFRISGRIEVVDASCLDA----TKFKKRAEAWSS 118
           +++ E   Q RI G+   V     DA      +  R  AW+S
Sbjct: 100 FFWPELERQVRIEGKAARVAPEVSDAYFASRPYTSRLGAWAS 139

[Close]
BLAST of Cp4.1LG03g15960 vs. Swiss-Prot
Match: PDXH_BURCM (Pyridoxine/pyridoxamine 5'-phosphate oxidase OS=Burkholderia ambifaria (strain ATCC BAA-244 / AMMD) GN=pdxH PE=3 SV=1)

HSP 1 Score: 60.5 bits (145), Expect = 2.5e-08
Identity = 37/102 (36.27%), Postives = 53/102 (51.96%), Query Frame = 1 

Query: 20  AQIKHSRFLHLATIGGNGRPSNRTVVFRGFEEGTDGIHIYTDSRSRKIEELKACPFAEIS 79
           A++     + LAT+G NGRPS R V+ +G +E   G   +T+  SRK  +L A P+A + 
Sbjct: 39  AKLPEPNTMTLATVGENGRPSARIVLIKGVDE--RGFVFFTNYESRKGRDLAAHPYAALL 98

Query: 80  WYFTESWEQFRISGRIEVVDASCLD----ATKFKKRAEAWSS 118
           +Y+ E   Q RI GRIE       D    +     R  AW+S
Sbjct: 99  FYWIELERQVRIEGRIEKTSTDESDRYFASRPLGSRIGAWAS 138

[Close]
BLAST of Cp4.1LG03g15960 vs. TrEMBL
Match: A0A0A0L745_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G119450 PE=4 SV=1)

HSP 1 Score: 342.4 bits (877), Expect = 3.6e-91
Identity = 164/197 (83.25%), Postives = 175/197 (88.83%), Query Frame = 1 

Query: 1   MGSAAAPWKSLLLSALESNAQIKHSRFLHLATIGGNGRPSNRTVVFRGFEEGTDGIHIYT 60
           MGSA APWKSLLLSALESN   KHS+FL LATIG NGRPSNRTVVFRGFEEGTD IHIYT
Sbjct: 1   MGSAVAPWKSLLLSALESNGHFKHSKFLQLATIGTNGRPSNRTVVFRGFEEGTDRIHIYT 60

Query: 61  DSRSRKIEELKACPFAEISWYFTESWEQFRISGRIEVVDASCLDATKFKKRAEAWSSISL 120
           DSR+RKIEELK CPFAEI WYFTESWEQFRISGR+EVVDASC+DATKFK+RAEAWSSIS 
Sbjct: 61  DSRNRKIEELKNCPFAEICWYFTESWEQFRISGRMEVVDASCVDATKFKQRAEAWSSISP 120

Query: 121 KSRMQYLGPSPNLPYIAEQPAKEPVLDPCSGPVDAFCLLVLDPDQVDYLNLKSEERKLFK 180
           KSRMQYLGPSP LP++AEQPAKEP LD CSGPVD FCLLV DPDQVDYLN++S+ER  FK
Sbjct: 121 KSRMQYLGPSPGLPHLAEQPAKEPFLDSCSGPVDTFCLLVFDPDQVDYLNVRSDERNSFK 180

Query: 181 AIPCLGRETLWESERIN 198
           AI   G E LWE+ERIN
Sbjct: 181 AITSFGGEKLWETERIN 197

[Close]
BLAST of Cp4.1LG03g15960 vs. TrEMBL
Match: A0A061DTI4_THECC (Pyridoxamine 5'-phosphate oxidase family protein isoform 1 OS=Theobroma cacao GN=TCM_005113 PE=4 SV=1)

HSP 1 Score: 265.8 bits (678), Expect = 4.3e-68
Identity = 129/198 (65.15%), Postives = 150/198 (75.76%), Query Frame = 1 

Query: 1   MGSAAAPWKSLLLSALESNAQIKHSRFLHLATIGGNGRPSNRTVVFRGFEEGTDGIHIYT 60
           MG+  APWK LLL+ALESN+ IKHS F  LATIG NGRPSNRTVVFRGF+E +D   I T
Sbjct: 1   MGTVTAPWKQLLLNALESNSHIKHSSFFQLATIGSNGRPSNRTVVFRGFQENSDKFQINT 60

Query: 61  DSRSRKIEELKACPFAEISWYFTESWEQFRISGRIEVVDASCLDATKFKKRAEAWSSISL 120
           DSRS KIEELK CPFAEI WYFT+SWEQFR SGRI+V+D S  +A K ++R ++W + S+
Sbjct: 61  DSRSHKIEELKHCPFAEICWYFTDSWEQFRFSGRIDVIDGSNPEALKLQQREKSWFASSV 120

Query: 121 KSRMQYLGPSPNLPYIAEQPAKEPVLDPCSGPVDAFCLLVLDPDQVDYLNLKSEERKLFK 180
           KSR+QYLGP P  P I+E P +E  LDP +GPV AFCLLVLDPDQVDYLNLKS +R  F 
Sbjct: 121 KSRLQYLGPIPGFPCISEHPNQEIALDPSTGPVGAFCLLVLDPDQVDYLNLKSNQRLKFI 180

Query: 181 AIPCLGRETLWESERINP 199
           +   L     W SERINP
Sbjct: 181 STLGLNGVKSWTSERINP 198

[Close]
BLAST of Cp4.1LG03g15960 vs. TrEMBL
Match: A0A067K081_JATCU (Uncharacterized protein OS=Jatropha curcas GN=JCGZ_14234 PE=4 SV=1)

HSP 1 Score: 261.5 bits (667), Expect = 8.1e-67
Identity = 121/196 (61.73%), Postives = 149/196 (76.02%), Query Frame = 1 

Query: 3   SAAAPWKSLLLSALESNAQIKHSRFLHLATIGGNGRPSNRTVVFRGFEEGTDGIHIYTDS 62
           SA  PWK LL+ ALESN+ +KHS F  LAT+G +GRPSNRTVVFRGFEE +D I I TD+
Sbjct: 5   SATTPWKQLLIRALESNSHLKHSSFFQLATVGSDGRPSNRTVVFRGFEENSDKIQINTDT 64

Query: 63  RSRKIEELKACPFAEISWYFTESWEQFRISGRIEVVDASCLDATKFKKRAEAWSSISLKS 122
           R+RK+EELK CPFAEI WYFT+SWEQFRI+GR++V+D S LD+ K + R ++W   S+KS
Sbjct: 65  RTRKVEELKQCPFAEICWYFTDSWEQFRINGRVDVIDGSNLDSVKLQLREKSWYDSSIKS 124

Query: 123 RMQYLGPSPNLPYIAEQPAKEPVLDPCSGPVDAFCLLVLDPDQVDYLNLKSEERKLFKAI 182
           RMQYLGP+P LP + E+P+ E  LDP SGPV  FCLL++DP+QVDYLNLKS +R +F   
Sbjct: 125 RMQYLGPNPGLPCLNERPSNEFFLDPSSGPVAPFCLLIVDPEQVDYLNLKSNQRIMFTFT 184

Query: 183 PCLGRETLWESERINP 199
                E  W S RINP
Sbjct: 185 QNANGENCWNSVRINP 200

[Close]
BLAST of Cp4.1LG03g15960 vs. TrEMBL
Match: M5X0N2_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa011702mg PE=4 SV=1)

HSP 1 Score: 261.2 bits (666), Expect = 1.1e-66
Identity = 121/196 (61.73%), Postives = 151/196 (77.04%), Query Frame = 1 

Query: 3   SAAAPWKSLLLSALESNAQIKHSRFLHLATIGGNGRPSNRTVVFRGFEEGTDGIHIYTDS 62
           +A APWK LLL+A+ESNA ++HS ++ LATI  NGRPSNRTVVFRGF+E +D I I TD 
Sbjct: 5   TAPAPWKQLLLNAMESNAHLRHSSYIQLATIACNGRPSNRTVVFRGFQEDSDKIQINTDG 64

Query: 63  RSRKIEELKACPFAEISWYFTESWEQFRISGRIEVVDASCLDATKFKKRAEAWSSISLKS 122
           RSRKIEELK CPFAEI WYFT+SWEQFRI+G ++++D S  D  K ++R ++W+S SLKS
Sbjct: 65  RSRKIEELKHCPFAEICWYFTDSWEQFRINGTVDIIDGSNPDPIKLQQRDKSWASSSLKS 124

Query: 123 RMQYLGPSPNLPYIAEQPAKEPVLDPCSGPVDAFCLLVLDPDQVDYLNLKSEERKLFKAI 182
           R+QYLGP P LP ++E+P KE  +DP +GPV AFC+LVLDP QVDYLNLKS +R  F + 
Sbjct: 125 RLQYLGPQPGLPNLSEEPPKEVSVDPSTGPVGAFCVLVLDPQQVDYLNLKSNQRLTFAST 184

Query: 183 PCLGRETLWESERINP 199
                E  W SER+NP
Sbjct: 185 GSPNEEKCWTSERVNP 200

[Close]
BLAST of Cp4.1LG03g15960 vs. TrEMBL
Match: A0A059CJ31_EUCGR (Uncharacterized protein OS=Eucalyptus grandis GN=EUGRSUZ_D02387 PE=4 SV=1)

HSP 1 Score: 257.3 bits (656), Expect = 1.5e-65
Identity = 124/200 (62.00%), Postives = 150/200 (75.00%), Query Frame = 1 

Query: 1   MGS--AAAPWKSLLLSALESNAQIKHSRFLHLATIGGNGRPSNRTVVFRGFEEGTDGIHI 60
           MGS  A APWK LLL AL SN+ +KHS F  LAT+G NGRP+NRTVVFRGF+EG+D I I
Sbjct: 32  MGSPTATAPWKQLLLDALRSNSSLKHSSFFQLATVGSNGRPANRTVVFRGFQEGSDKIQI 91

Query: 61  YTDSRSRKIEELKACPFAEISWYFTESWEQFRISGRIEVVDASCLDATKFKKRAEAWSSI 120
           +TDSRSRKIEELK  P AEI WYFT+SWEQFRISG +E++D S  D+ K ++R +AW + 
Sbjct: 92  HTDSRSRKIEELKHNPLAEICWYFTDSWEQFRISGIVEMIDHSNPDSLKLQQREKAWHAC 151

Query: 121 SLKSRMQYLGPSPNLPYIAEQPAKEPVLDPCSGPVDAFCLLVLDPDQVDYLNLKSEERKL 180
           S+KSR+QYLGP+P LPYI E    E  LD  + PVD+FCLL++DPDQVDYLNLKS +R  
Sbjct: 152 SVKSRLQYLGPTPGLPYIGEHLTDENSLDSSTSPVDSFCLLIMDPDQVDYLNLKSNQRMT 211

Query: 181 FKAIPCLGRETLWESERINP 199
           F +     +E  W  ERINP
Sbjct: 212 FTSTQSDSQEKCWALERINP 231

[Close]
BLAST of Cp4.1LG03g15960 vs. TAIR10
Match: AT2G46580.1 (AT2G46580.1 Pyridoxamine 5'-phosphate oxidase family protein)

HSP 1 Score: 240.7 bits (613), Expect = 7.5e-64
Identity = 112/198 (56.57%), Postives = 150/198 (75.76%), Query Frame = 1 

Query: 1   MGSAAAPWKSLLLSALESNAQIKHSRFLHLATIGGNGRPSNRTVVFRGFEEGTDGIHIYT 60
           MG+  APWK LL  A+E+N+ + HS ++ LATIG NGRPSNRTVVFRGFEE +D I I T
Sbjct: 1   MGTHVAPWKQLLFGAIEANSHLSHSSYVQLATIGLNGRPSNRTVVFRGFEENSDRIQINT 60

Query: 61  DSRSRKIEELKACPFAEISWYFTESWEQFRISGRIEVVDASCLDATKFKKRAEAWSSISL 120
           D RSRKIEELK CPF+E+ WYF+++WEQFRI+GRIEV+DAS  D TK ++R +AW + SL
Sbjct: 61  DLRSRKIEELKHCPFSEMCWYFSDTWEQFRINGRIEVIDASNPDQTKLQQREKAWFANSL 120

Query: 121 KSRMQYLGPSPNLPYIAEQPAKEPVLDPCSGPVDAFCLLVLDPDQVDYLNLKSEERKLFK 180
           +SR+ Y+ P+P  P  +EQ +++  LDP SGPV  +CLL+L+P++VDYLNLK+ +R  F 
Sbjct: 121 RSRLIYVCPTPGSPCNSEQSSQQVKLDPSSGPVPEYCLLLLEPEKVDYLNLKTNQRLFFS 180

Query: 181 AIPCLGRETLWESERINP 199
           ++     E  W SE++NP
Sbjct: 181 SMATGTGEKCWTSEKVNP 198

[Close]
BLAST of Cp4.1LG03g15960 vs. NCBI nr
Match: gi|659074927|ref|XP_008437871.1| (PREDICTED: pyridoxine/pyridoxamine 5'-phosphate oxidase 2 [Cucumis melo])

HSP 1 Score: 348.2 bits (892), Expect = 9.4e-93
Identity = 168/198 (84.85%), Postives = 178/198 (89.90%), Query Frame = 1 

Query: 1   MGSAAAPWKSLLLSALESNAQIKHSRFLHLATIGGNGRPSNRTVVFRGFEEGTDGIHIYT 60
           MGSA APWKSLLL ALESN QIKHS+FL LATIG NGRPSNRTVVFR FEEGTD IHIYT
Sbjct: 1   MGSAVAPWKSLLLRALESNGQIKHSKFLQLATIGTNGRPSNRTVVFREFEEGTDRIHIYT 60

Query: 61  DSRSRKIEELKACPFAEISWYFTESWEQFRISGRIEVVDASCLDATKFKKRAEAWSSISL 120
           DSRSRKIEELK CPFAE+ WYFT++WEQFRISGR+EVVDASCLDATKFK+RAEAWSSIS 
Sbjct: 61  DSRSRKIEELKNCPFAEVCWYFTDTWEQFRISGRMEVVDASCLDATKFKQRAEAWSSISP 120

Query: 121 KSRMQYLGPSPNLPYIAEQPAKEPVLDPCSGPVDAFCLLVLDPDQVDYLNLKSEERKLFK 180
           KSRMQYLGPSP LP++AEQPAKEP LD  SGPVDAFCLLV DPDQVDYLNLKSEER LF+
Sbjct: 121 KSRMQYLGPSPGLPHLAEQPAKEPFLDSYSGPVDAFCLLVFDPDQVDYLNLKSEERNLFR 180

Query: 181 AIPCLGRETLWESERINP 199
           AIP  G E LWE+ERINP
Sbjct: 181 AIPSFGGEKLWETERINP 198

[Close]
BLAST of Cp4.1LG03g15960 vs. NCBI nr
Match: gi|449432030|ref|XP_004133803.1| (PREDICTED: pyridoxine/pyridoxamine 5'-phosphate oxidase 2 [Cucumis sativus])

HSP 1 Score: 342.4 bits (877), Expect = 5.2e-91
Identity = 164/197 (83.25%), Postives = 175/197 (88.83%), Query Frame = 1 

Query: 1   MGSAAAPWKSLLLSALESNAQIKHSRFLHLATIGGNGRPSNRTVVFRGFEEGTDGIHIYT 60
           MGSA APWKSLLLSALESN   KHS+FL LATIG NGRPSNRTVVFRGFEEGTD IHIYT
Sbjct: 1   MGSAVAPWKSLLLSALESNGHFKHSKFLQLATIGTNGRPSNRTVVFRGFEEGTDRIHIYT 60

Query: 61  DSRSRKIEELKACPFAEISWYFTESWEQFRISGRIEVVDASCLDATKFKKRAEAWSSISL 120
           DSR+RKIEELK CPFAEI WYFTESWEQFRISGR+EVVDASC+DATKFK+RAEAWSSIS 
Sbjct: 61  DSRNRKIEELKNCPFAEICWYFTESWEQFRISGRMEVVDASCVDATKFKQRAEAWSSISP 120

Query: 121 KSRMQYLGPSPNLPYIAEQPAKEPVLDPCSGPVDAFCLLVLDPDQVDYLNLKSEERKLFK 180
           KSRMQYLGPSP LP++AEQPAKEP LD CSGPVD FCLLV DPDQVDYLN++S+ER  FK
Sbjct: 121 KSRMQYLGPSPGLPHLAEQPAKEPFLDSCSGPVDTFCLLVFDPDQVDYLNVRSDERNSFK 180

Query: 181 AIPCLGRETLWESERIN 198
           AI   G E LWE+ERIN
Sbjct: 181 AITSFGGEKLWETERIN 197

[Close]
BLAST of Cp4.1LG03g15960 vs. NCBI nr
Match: gi|590721072|ref|XP_007051505.1| (Pyridoxamine 5'-phosphate oxidase family protein isoform 1 [Theobroma cacao])

HSP 1 Score: 265.8 bits (678), Expect = 6.2e-68
Identity = 129/198 (65.15%), Postives = 150/198 (75.76%), Query Frame = 1 

Query: 1   MGSAAAPWKSLLLSALESNAQIKHSRFLHLATIGGNGRPSNRTVVFRGFEEGTDGIHIYT 60
           MG+  APWK LLL+ALESN+ IKHS F  LATIG NGRPSNRTVVFRGF+E +D   I T
Sbjct: 1   MGTVTAPWKQLLLNALESNSHIKHSSFFQLATIGSNGRPSNRTVVFRGFQENSDKFQINT 60

Query: 61  DSRSRKIEELKACPFAEISWYFTESWEQFRISGRIEVVDASCLDATKFKKRAEAWSSISL 120
           DSRS KIEELK CPFAEI WYFT+SWEQFR SGRI+V+D S  +A K ++R ++W + S+
Sbjct: 61  DSRSHKIEELKHCPFAEICWYFTDSWEQFRFSGRIDVIDGSNPEALKLQQREKSWFASSV 120

Query: 121 KSRMQYLGPSPNLPYIAEQPAKEPVLDPCSGPVDAFCLLVLDPDQVDYLNLKSEERKLFK 180
           KSR+QYLGP P  P I+E P +E  LDP +GPV AFCLLVLDPDQVDYLNLKS +R  F 
Sbjct: 121 KSRLQYLGPIPGFPCISEHPNQEIALDPSTGPVGAFCLLVLDPDQVDYLNLKSNQRLKFI 180

Query: 181 AIPCLGRETLWESERINP 199
           +   L     W SERINP
Sbjct: 181 STLGLNGVKSWTSERINP 198

[Close]
BLAST of Cp4.1LG03g15960 vs. NCBI nr
Match: gi|470141867|ref|XP_004306647.1| (PREDICTED: pyridoxine/pyridoxamine 5'-phosphate oxidase 2 [Fragaria vesca subsp. vesca])

HSP 1 Score: 263.1 bits (671), Expect = 4.0e-67
Identity = 124/196 (63.27%), Postives = 151/196 (77.04%), Query Frame = 1 

Query: 3   SAAAPWKSLLLSALESNAQIKHSRFLHLATIGGNGRPSNRTVVFRGFEEGTDGIHIYTDS 62
           +A APWK LLLSALE+NA ++HS +  LATIG NGRPSNRTVVFRGF+E +D I I TD 
Sbjct: 5   TAPAPWKQLLLSALEANAHLRHSSYFQLATIGSNGRPSNRTVVFRGFQEDSDKIQINTDC 64

Query: 63  RSRKIEELKACPFAEISWYFTESWEQFRISGRIEVVDASCLDATKFKKRAEAWSSISLKS 122
           R+RKIEELK CPFAEI WYFT+SWEQFRI+G ++++D S  D  K ++R ++W + SL+S
Sbjct: 65  RTRKIEELKHCPFAEICWYFTDSWEQFRINGTVDIIDGSNSDPMKLQQREKSWFAGSLRS 124

Query: 123 RMQYLGPSPNLPYIAEQPAKEPVLDPCSGPVDAFCLLVLDPDQVDYLNLKSEERKLFKAI 182
           R+QYLGPSP LP  +EQPA E  LDP +GPV AFCLLVLDP QVDYLNLKS +R  F + 
Sbjct: 125 RLQYLGPSPGLPNPSEQPANEVSLDPSTGPVGAFCLLVLDPHQVDYLNLKSNQRLSFTST 184

Query: 183 PCLGRETLWESERINP 199
           P    E  W SE++NP
Sbjct: 185 PSSDGEKSWTSEKVNP 200

[Close]
BLAST of Cp4.1LG03g15960 vs. NCBI nr
Match: gi|643716837|gb|KDP28463.1| (hypothetical protein JCGZ_14234 [Jatropha curcas])

HSP 1 Score: 261.5 bits (667), Expect = 1.2e-66
Identity = 121/196 (61.73%), Postives = 149/196 (76.02%), Query Frame = 1 

Query: 3   SAAAPWKSLLLSALESNAQIKHSRFLHLATIGGNGRPSNRTVVFRGFEEGTDGIHIYTDS 62
           SA  PWK LL+ ALESN+ +KHS F  LAT+G +GRPSNRTVVFRGFEE +D I I TD+
Sbjct: 5   SATTPWKQLLIRALESNSHLKHSSFFQLATVGSDGRPSNRTVVFRGFEENSDKIQINTDT 64

Query: 63  RSRKIEELKACPFAEISWYFTESWEQFRISGRIEVVDASCLDATKFKKRAEAWSSISLKS 122
           R+RK+EELK CPFAEI WYFT+SWEQFRI+GR++V+D S LD+ K + R ++W   S+KS
Sbjct: 65  RTRKVEELKQCPFAEICWYFTDSWEQFRINGRVDVIDGSNLDSVKLQLREKSWYDSSIKS 124

Query: 123 RMQYLGPSPNLPYIAEQPAKEPVLDPCSGPVDAFCLLVLDPDQVDYLNLKSEERKLFKAI 182
           RMQYLGP+P LP + E+P+ E  LDP SGPV  FCLL++DP+QVDYLNLKS +R +F   
Sbjct: 125 RMQYLGPNPGLPCLNERPSNEFFLDPSSGPVAPFCLLIVDPEQVDYLNLKSNQRIMFTFT 184

Query: 183 PCLGRETLWESERINP 199
                E  W S RINP
Sbjct: 185 QNANGENCWNSVRINP 200

The following BLAST results are available for this feature:
BLAST of Cp4.1LG03g15960 vs. Swiss-Prot
Match NameE-valueIdentityDescription
PPOX2_ARATH1.3e-6256.57Pyridoxine/pyridoxamine 5'-phosphate oxidase 2 OS=Arabidopsis thaliana GN=PPOX2 ... [more]
PDXH_MYXXA6.5e-0926.46Pyridoxine/pyridoxamine 5'-phosphate oxidase OS=Myxococcus xanthus GN=pdxH PE=1 ... [more]
PDXH_MYXXD6.5e-0926.46Pyridoxine/pyridoxamine 5'-phosphate oxidase OS=Myxococcus xanthus (strain DK 16... [more]
PDXH_CHRVO1.1e-0834.31Pyridoxine/pyridoxamine 5'-phosphate oxidase OS=Chromobacterium violaceum (strai... [more]
PDXH_BURCM2.5e-0836.27Pyridoxine/pyridoxamine 5'-phosphate oxidase OS=Burkholderia ambifaria (strain A... [more]
BLAST of Cp4.1LG03g15960 vs. TrEMBL
Match NameE-valueIdentityDescription
A0A0A0L745_CUCSA3.6e-9183.25Uncharacterized protein OS=Cucumis sativus GN=Csa_3G119450 PE=4 SV=1[more]
A0A061DTI4_THECC4.3e-6865.15Pyridoxamine 5'-phosphate oxidase family protein isoform 1 OS=Theobroma cacao GN... [more]
A0A067K081_JATCU8.1e-6761.73Uncharacterized protein OS=Jatropha curcas GN=JCGZ_14234 PE=4 SV=1[more]
M5X0N2_PRUPE1.1e-6661.73Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa011702mg PE=4 SV=1[more]
A0A059CJ31_EUCGR1.5e-6562.00Uncharacterized protein OS=Eucalyptus grandis GN=EUGRSUZ_D02387 PE=4 SV=1[more]
BLAST of Cp4.1LG03g15960 vs. TAIR10
Match NameE-valueIdentityDescription
AT2G46580.17.5e-6456.57 Pyridoxamine 5'-phosphate oxidase family protein[more]
BLAST of Cp4.1LG03g15960 vs. NCBI nr
Match NameE-valueIdentityDescription
gi|659074927|ref|XP_008437871.1|9.4e-9384.85PREDICTED: pyridoxine/pyridoxamine 5'-phosphate oxidase 2 [Cucumis melo][more]
gi|449432030|ref|XP_004133803.1|5.2e-9183.25PREDICTED: pyridoxine/pyridoxamine 5'-phosphate oxidase 2 [Cucumis sativus][more]
gi|590721072|ref|XP_007051505.1|6.2e-6865.15Pyridoxamine 5'-phosphate oxidase family protein isoform 1 [Theobroma cacao][more]
gi|470141867|ref|XP_004306647.1|4.0e-6763.27PREDICTED: pyridoxine/pyridoxamine 5'-phosphate oxidase 2 [Fragaria vesca subsp.... [more]
gi|643716837|gb|KDP28463.1|1.2e-6661.73hypothetical protein JCGZ_14234 [Jatropha curcas][more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: Biological Process
TermDefinition
GO:0055114oxidation-reduction process
Vocabulary: Molecular Function
TermDefinition
GO:0016491oxidoreductase activity
GO:0010181FMN binding
Vocabulary: INTERPRO
TermDefinition
IPR024624Pyridox_Oxase_Alr4036_FMN-bd
IPR024015Pyridox_Oxase_FMN-dep_Alr4036
IPR012349Split_barrel_FMN-bd
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0055114 oxidation-reduction process
biological_process GO:0042823 pyridoxal phosphate biosynthetic process
biological_process GO:0008615 pyridoxine biosynthetic process
biological_process GO:0009443 pyridoxal 5'-phosphate salvage
biological_process GO:0008150 biological_process
cellular_component GO:0005575 cellular_component
molecular_function GO:0010181 FMN binding
molecular_function GO:0004733 pyridoxamine-phosphate oxidase activity
molecular_function GO:0016491 oxidoreductase activity
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG03g15960.1Cp4.1LG03g15960.1mRNA


InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo
Date Performed: 2017-12-02
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR012349FMN-binding split barrelGENE3DG3DSA:2.30.110.10coord: 5..198
score: 3.7
IPR012349FMN-binding split barrelunknownSSF50475FMN-binding split barrelcoord: 3..198
score: 6.0
IPR024015Pyridoxamine 5'-phosphate oxidase, probable FMN-dependent, Alr4036 familyTIGRFAMsTIGR04026TIGR04026coord: 6..198
score: 1.9
IPR024624Pyridoxamine 5'-phosphate oxidase, Alr4036 family, FMN-binding domainPFAMPF12766Pyridox_oxase_2coord: 6..98
score: 4.1
NoneNo IPR availablePANTHERPTHR10851PYRIDOXINE-5-PHOSPHATE OXIDASEcoord: 6..198
score: 1.3
NoneNo IPR availablePANTHERPTHR10851:SF3SUBFAMILY NOT NAMEDcoord: 6..198
score: 1.3

Synteny
The following gene(s) are orthologous to this gene:
GeneOrthologueOrganismBlock
Cp4.1LG03g15960Cucsa.259350Cucumber (Gy14) v1cgycpeB0682
Cp4.1LG03g15960CmaCh14G021190Cucurbita maxima (Rimu)cmacpeB283
Cp4.1LG03g15960CmoCh14G021800Cucurbita moschata (Rifu)cmocpeB245
Cp4.1LG03g15960Cla021143Watermelon (97103) v1cpewmB613
Cp4.1LG03g15960Csa3G119450Cucumber (Chinese Long) v2cpecuB609
Cp4.1LG03g15960MELO3C006011Melon (DHL92) v3.5.1cpemeB585
Cp4.1LG03g15960ClCG05G000710Watermelon (Charleston Gray)cpewcgB559
Cp4.1LG03g15960CSPI03G07810Wild cucumber (PI 183967)cpecpiB611
Cp4.1LG03g15960Lsi05G021350Bottle gourd (USVL1VR-Ls)cpelsiB506
Cp4.1LG03g15960MELO3C006011.2Melon (DHL92) v3.6.1cpemedB693
Cp4.1LG03g15960CsaV3_3G007930Cucumber (Chinese Long) v3cpecucB0759
Cp4.1LG03g15960Bhi01G000367Wax gourdcpewgoB0770
Cp4.1LG03g15960CsGy3G007960Cucumber (Gy14) v2cgybcpeB406
Cp4.1LG03g15960Carg25704Silver-seed gourdcarcpeB0179
The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:
GeneOrganismBlock
Cp4.1LG03g15960Cucumber (Chinese Long) v3cpecucB0774
Cp4.1LG03g15960Wax gourdcpewgoB0761
Cp4.1LG03g15960Cucurbita pepo (Zucchini)cpecpeB217
Cp4.1LG03g15960Cucurbita pepo (Zucchini)cpecpeB479
Cp4.1LG03g15960Cucurbita pepo (Zucchini)cpecpeB482
Cp4.1LG03g15960Cucumber (Gy14) v1cgycpeB0574
Cp4.1LG03g15960Cucurbita maxima (Rimu)cmacpeB639
Cp4.1LG03g15960Cucurbita maxima (Rimu)cmacpeB839
Cp4.1LG03g15960Cucurbita moschata (Rifu)cmocpeB589
Cp4.1LG03g15960Cucurbita moschata (Rifu)cmocpeB787
Cp4.1LG03g15960Wild cucumber (PI 183967)cpecpiB624
Cp4.1LG03g15960Cucumber (Chinese Long) v2cpecuB622
Cp4.1LG03g15960Bottle gourd (USVL1VR-Ls)cpelsiB486
Cp4.1LG03g15960Watermelon (97103) v1cpewmB616
Cp4.1LG03g15960Cucumber (Gy14) v2cgybcpeB678
Cp4.1LG03g15960Melon (DHL92) v3.6.1cpemedB651
Cp4.1LG03g15960Silver-seed gourdcarcpeB1379