Carg25704 (gene) Silver-seed gourd | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Carg25704
Type	gene
Organism	Cucurbita argyrosperma (Silver-seed gourd)
Description	Pyridoxamine 5'-phosphate oxidase family protein
Location	Cucurbita_argyrosperma_scaffold_341 : 66635 .. 68239 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGGCGAATACATAGCACAGCAGTAGGCGAACTAGCGGGCGATTTGGAAATGGGATCGGCAGCGGCGCCATGGAAGTCCCTTCTTCTCAGTGCTTTGGAATCTAATGCCCAAATCAAGCATTCCACATTCTTGCACCTCGTACATTTCCCTCTCTCTATTTCTCTTCAACTTGAACTACCCTTTCCCTTTTTCACAGTCGGGTTTTATTTTCTTAAATGGAATTTGTCTTCAGGCAACGATTGGAGCCAATGGAAGGCCATCAAATCGCACCGTTGTTTTCAGAGGATTTGAAGAGGGCACCGATAGGATCCATATTTACACGGATTCAAGAAGTCGCAAGGTTCTTCTACGATGAAGCTCTATATTTCGCTTTAGATCGCTCAATTCATACGCCCATTTCTTGTTGTTCTATGGTTTCCGACGCAATCGTTATCTGGGTTTCTGGATTTTCAATTATGTAATTCATATGTTTGGCTACAATAAGATAAGCTATAAAATATGCTCAGTACTGGGAATTTGTCTGCGATGGATTAGGTCAGGTTTGTGATATTAAGCATCTGAGTTTTGTCCTGCAAATGAACTTTTCGCTTTTGGTTGATTCTGCAGATTGAAGAGCTTAAAACTTGTCCATTTGCTGAGGTAACTATTCTCGTCTCTTCCTGCCTCTTAATACAGTTCATAAAACAAATGATTTGTTCTTGCTGGTCTATGTAGCTTATCTTATTTACATAACTGCAAATATGTGCCTATTTGAGTCAAATTTTGGACTTGGAATGTATTTGTTGTTCAAGATAAGTTGGTATTTCACGGAATCATGGGAGCAATTTCGTATCAGTGGAAGAATTGAGGTTGTTGATGCGTCATGTCTTGATGCTACAAAATTTAAGGTTGTGGGCATGACCCTTTCCTCTTATAACTTACAACTTGTTCACTTTCTTGTTCACTTCATTCAACTCAGTATAATCTTATATATTTATTAATGCAGAAAAGAGCAGAAGCCTGGTCCTCCATTTCTCCAAAGTCAAGAATGCAGTATTTGGGGCCTAGTCCAAATCTTCCCTATATTGCCGAACAGCCAGCCAAGGAACCTGTTCTAGATTCTTGTTCAGGTCCCGTTGATGCATTTTGTTTGCTGGTCTTGGATCCTGATCAGGTTTGTAGTATATATATATGTATATAGACTTTACGTTATGTTACATTTGACCGCGTTTCGCTCTCAGGTCGATTACTTGAATCTGAAGAGTGCAGAAAGGAAGTTGTTTAAAGCTATTCCATGTGTTGGAAGGGAGACATTATGGGAATCAGAGCGGATAAACCCTTAGCAATTGCACCTCGATTTGCAGGTATGTTGATAACAACAAGCTGCTACGTTGAAATGAAGTGATATAGTTAGTGAGTCATGTGTTGAAAATAATCCTCCGACCCTTTGGTTGTTAGTAGAGATGATACTCTGATTGAACAATCATGGTTCCCTATATCTGTAGATAGGGTGTATGTAACTCAATTCTTATGCTGTTGAAGCTGAAACAAAAGGAATGAAAGTTGGAATGAGTTGTTTGAAGCATGAAATATGCAAAGAAACAGAAGGATAGGAAAGGCTCT

mRNA sequence

ATGGGGCGAATACATAGCACAGCAGTAGGCGAACTAGCGGGCGATTTGGAAATGGGATCGGCAGCGGCGCCATGGAAGTCCCTTCTTCTCAGTGCTTTGGAATCTAATGCCCAAATCAAGCATTCCACATTCTTGCACCTCGCAACGATTGGAGCCAATGGAAGGCCATCAAATCGCACCGTTGTTTTCAGAGGATTTGAAGAGGGCACCGATAGGATCCATATTTACACGGATTCAAGAAGTCGCAAGATTGAAGAGCTTAAAACTTGTCCATTTGCTGAGATAAGTTGGTATTTCACGGAATCATGGGAGCAATTTCGTATCAGTGGAAGAATTGAGGTTGTTGATGCGTCATGTCTTGATGCTACAAAATTTAAGAAAAGAGCAGAAGCCTGGTCCTCCATTTCTCCAAAGTCAAGAATGCAGTATTTGGGGCCTAGTCCAAATCTTCCCTATATTGCCGAACAGCCAGCCAAGGAACCTGTTCTAGATTCTTGTTCAGGTCCCGTTGATGCATTTTGTTTGCTGGTCTTGGATCCTGATCAGGTCGATTACTTGAATCTGAAGAGTGCAGAAAGGAAGTTGTTTAAAGCTATTCCATGTGTTGGAAGGGAGACATTATGGGAATCAGAGCGGATAAACCCTTAGCAATTGCACCTCGATTTGCAGGTATGTTGATAACAACAAGCTGCTACGTTGAAATGAAGTGATATAGTTAGTGAGTCATGTGTTGAAAATAATCCTCCGACCCTTTGGTTGTTAGTAGAGATGATACTCTGATTGAACAATCATGGTTCCCTATATCTGTAGATAGGGTGTATGTAACTCAATTCTTATGCTGTTGAAGCTGAAACAAAAGGAATGAAAGTTGGAATGAGTTGTTTGAAGCATGAAATATGCAAAGAAACAGAAGGATAGGAAAGGCTCT

Coding sequence (CDS)

ATGGGGCGAATACATAGCACAGCAGTAGGCGAACTAGCGGGCGATTTGGAAATGGGATCGGCAGCGGCGCCATGGAAGTCCCTTCTTCTCAGTGCTTTGGAATCTAATGCCCAAATCAAGCATTCCACATTCTTGCACCTCGCAACGATTGGAGCCAATGGAAGGCCATCAAATCGCACCGTTGTTTTCAGAGGATTTGAAGAGGGCACCGATAGGATCCATATTTACACGGATTCAAGAAGTCGCAAGATTGAAGAGCTTAAAACTTGTCCATTTGCTGAGATAAGTTGGTATTTCACGGAATCATGGGAGCAATTTCGTATCAGTGGAAGAATTGAGGTTGTTGATGCGTCATGTCTTGATGCTACAAAATTTAAGAAAAGAGCAGAAGCCTGGTCCTCCATTTCTCCAAAGTCAAGAATGCAGTATTTGGGGCCTAGTCCAAATCTTCCCTATATTGCCGAACAGCCAGCCAAGGAACCTGTTCTAGATTCTTGTTCAGGTCCCGTTGATGCATTTTGTTTGCTGGTCTTGGATCCTGATCAGGTCGATTACTTGAATCTGAAGAGTGCAGAAAGGAAGTTGTTTAAAGCTATTCCATGTGTTGGAAGGGAGACATTATGGGAATCAGAGCGGATAAACCCTTAG

Protein sequence

MGRIHSTAVGELAGDLEMGSAAAPWKSLLLSALESNAQIKHSTFLHLATIGANGRPSNRTVVFRGFEEGTDRIHIYTDSRSRKIEELKTCPFAEISWYFTESWEQFRISGRIEVVDASCLDATKFKKRAEAWSSISPKSRMQYLGPSPNLPYIAEQPAKEPVLDSCSGPVDAFCLLVLDPDQVDYLNLKSAERKLFKAIPCVGRETLWESERINP

Homology

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BLAST of Carg25704 vs. NCBI nr
Match: XP_022924710.1 (pyridoxine/pyridoxamine 5'-phosphate oxidase 2 isoform X1 [Cucurbita moschata])

HSP 1 Score: 441.0 bits (1133), Expect = 2.3e-120
Identity = 215/215 (100.00%), Postives = 215/215 (100.00%), Query Frame = 0


Query: 1   MGRIHSTAVGELAGDLEMGSAAAPWKSLLLSALESNAQIKHSTFLHLATIGANGRPSNRT 60
           MGRIHSTAVGELAGDLEMGSAAAPWKSLLLSALESNAQIKHSTFLHLATIGANGRPSNRT
Sbjct: 7   MGRIHSTAVGELAGDLEMGSAAAPWKSLLLSALESNAQIKHSTFLHLATIGANGRPSNRT 66

Query: 61  VVFRGFEEGTDRIHIYTDSRSRKIEELKTCPFAEISWYFTESWEQFRISGRIEVVDASCL 120
           VVFRGFEEGTDRIHIYTDSRSRKIEELKTCPFAEISWYFTESWEQFRISGRIEVVDASCL
Sbjct: 67  VVFRGFEEGTDRIHIYTDSRSRKIEELKTCPFAEISWYFTESWEQFRISGRIEVVDASCL 126

Query: 121 DATKFKKRAEAWSSISPKSRMQYLGPSPNLPYIAEQPAKEPVLDSCSGPVDAFCLLVLDP 180
           DATKFKKRAEAWSSISPKSRMQYLGPSPNLPYIAEQPAKEPVLDSCSGPVDAFCLLVLDP
Sbjct: 127 DATKFKKRAEAWSSISPKSRMQYLGPSPNLPYIAEQPAKEPVLDSCSGPVDAFCLLVLDP 186

Query: 181 DQVDYLNLKSAERKLFKAIPCVGRETLWESERINP 216
           DQVDYLNLKSAERKLFKAIPCVGRETLWESERINP
Sbjct: 187 DQVDYLNLKSAERKLFKAIPCVGRETLWESERINP 221

[Close]

BLAST of Carg25704 vs. NCBI nr
Match: XP_022980848.1 (pyridoxine/pyridoxamine 5'-phosphate oxidase 2 isoform X1 [Cucurbita maxima])

HSP 1 Score: 395.2 bits (1014), Expect = 1.4e-106
Identity = 192/198 (96.97%), Postives = 193/198 (97.47%), Query Frame = 0


Query: 18  MGSAAAPWKSLLLSALESNAQIKHSTFLHLATIGANGRPSNRTVVFRGFEEGTDRIHIYT 77
           MGSAAAPWKSLLLSALESNAQI HS FLHLATIG NGRPSNRTVVFRGFEEGTD I IYT
Sbjct: 1   MGSAAAPWKSLLLSALESNAQITHSRFLHLATIGGNGRPSNRTVVFRGFEEGTDGIRIYT 60

Query: 78  DSRSRKIEELKTCPFAEISWYFTESWEQFRISGRIEVVDASCLDATKFKKRAEAWSSISP 137
           DSRSRKIEELKTCPFAEISWYFTESWEQFRISGRIEVVDASCLDATKFKKRAEAWSSISP
Sbjct: 61  DSRSRKIEELKTCPFAEISWYFTESWEQFRISGRIEVVDASCLDATKFKKRAEAWSSISP 120

Query: 138 KSRMQYLGPSPNLPYIAEQPAKEPVLDSCSGPVDAFCLLVLDPDQVDYLNLKSAERKLFK 197
           KSRMQYLGPSPNLPYIAEQPAKEPVLDSCSGPVDAFCLLVLDPDQVDYLNLKSAERKLFK
Sbjct: 121 KSRMQYLGPSPNLPYIAEQPAKEPVLDSCSGPVDAFCLLVLDPDQVDYLNLKSAERKLFK 180

Query: 198 AIPCVGRETLWESERINP 216
           AIPC+GRETLWESERINP
Sbjct: 181 AIPCLGRETLWESERINP 198

[Close]

BLAST of Carg25704 vs. NCBI nr
Match: XP_023527386.1 (pyridoxine/pyridoxamine 5'-phosphate oxidase 2 isoform X1 [Cucurbita pepo subsp. pepo] >XP_023527387.1 pyridoxine/pyridoxamine 5'-phosphate oxidase 2 isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 391.0 bits (1003), Expect = 2.7e-105
Identity = 190/198 (95.96%), Postives = 191/198 (96.46%), Query Frame = 0


Query: 18  MGSAAAPWKSLLLSALESNAQIKHSTFLHLATIGANGRPSNRTVVFRGFEEGTDRIHIYT 77
           MGSAAAPWKSLLLSALESNAQIKHS FLHLATIG NGRPSNRTVVFRGFEEGTD IHIYT
Sbjct: 1   MGSAAAPWKSLLLSALESNAQIKHSRFLHLATIGGNGRPSNRTVVFRGFEEGTDGIHIYT 60

Query: 78  DSRSRKIEELKTCPFAEISWYFTESWEQFRISGRIEVVDASCLDATKFKKRAEAWSSISP 137
           DSRSRKIEELK CPFAEISWYFTESWEQFRISGRIEVVDASCLDATKFKKRAEAWSSIS 
Sbjct: 61  DSRSRKIEELKACPFAEISWYFTESWEQFRISGRIEVVDASCLDATKFKKRAEAWSSISL 120

Query: 138 KSRMQYLGPSPNLPYIAEQPAKEPVLDSCSGPVDAFCLLVLDPDQVDYLNLKSAERKLFK 197
           KSRMQYLGPSPNLPYIAEQPAKEPVLD CSGPVDAFCLLVLDPDQVDYLNLKS ERKLFK
Sbjct: 121 KSRMQYLGPSPNLPYIAEQPAKEPVLDPCSGPVDAFCLLVLDPDQVDYLNLKSEERKLFK 180

Query: 198 AIPCVGRETLWESERINP 216
           AIPC+GRETLWESERINP
Sbjct: 181 AIPCLGRETLWESERINP 198

[Close]

BLAST of Carg25704 vs. NCBI nr
Match: XP_022924711.1 (pyridoxine/pyridoxamine 5'-phosphate oxidase 2 isoform X2 [Cucurbita moschata])

HSP 1 Score: 357.8 bits (917), Expect = 2.5e-95
Identity = 183/215 (85.12%), Postives = 183/215 (85.12%), Query Frame = 0


Query: 1   MGRIHSTAVGELAGDLEMGSAAAPWKSLLLSALESNAQIKHSTFLHLATIGANGRPSNRT 60
           MGRIHSTAVGELAGDLEMGSAAAPWKSLLLSALESNAQIKHSTFLHLATIGANGRPSNRT
Sbjct: 7   MGRIHSTAVGELAGDLEMGSAAAPWKSLLLSALESNAQIKHSTFLHLATIGANGRPSNRT 66

Query: 61  VVFRGFEEGTDRIHIYTDSRSRKIEELKTCPFAEISWYFTESWEQFRISGRIEVVDASCL 120
           VVFRGFEEGTDRIHIYTDSRSRKIEELKTCPFAE                          
Sbjct: 67  VVFRGFEEGTDRIHIYTDSRSRKIEELKTCPFAE-------------------------- 126

Query: 121 DATKFKKRAEAWSSISPKSRMQYLGPSPNLPYIAEQPAKEPVLDSCSGPVDAFCLLVLDP 180
                 KRAEAWSSISPKSRMQYLGPSPNLPYIAEQPAKEPVLDSCSGPVDAFCLLVLDP
Sbjct: 127 ------KRAEAWSSISPKSRMQYLGPSPNLPYIAEQPAKEPVLDSCSGPVDAFCLLVLDP 186

Query: 181 DQVDYLNLKSAERKLFKAIPCVGRETLWESERINP 216
           DQVDYLNLKSAERKLFKAIPCVGRETLWESERINP
Sbjct: 187 DQVDYLNLKSAERKLFKAIPCVGRETLWESERINP 189

[Close]

BLAST of Carg25704 vs. NCBI nr
Match: XP_008437871.1 (PREDICTED: pyridoxine/pyridoxamine 5'-phosphate oxidase 2 [Cucumis melo] >XP_016898998.1 PREDICTED: pyridoxine/pyridoxamine 5'-phosphate oxidase 2 [Cucumis melo])

HSP 1 Score: 354.4 bits (908), Expect = 2.8e-94
Identity = 170/198 (85.86%), Postives = 179/198 (90.40%), Query Frame = 0


Query: 18  MGSAAAPWKSLLLSALESNAQIKHSTFLHLATIGANGRPSNRTVVFRGFEEGTDRIHIYT 77
           MGSA APWKSLLL ALESN QIKHS FL LATIG NGRPSNRTVVFR FEEGTDRIHIYT
Sbjct: 1   MGSAVAPWKSLLLRALESNGQIKHSKFLQLATIGTNGRPSNRTVVFREFEEGTDRIHIYT 60

Query: 78  DSRSRKIEELKTCPFAEISWYFTESWEQFRISGRIEVVDASCLDATKFKKRAEAWSSISP 137
           DSRSRKIEELK CPFAE+ WYFT++WEQFRISGR+EVVDASCLDATKFK+RAEAWSSISP
Sbjct: 61  DSRSRKIEELKNCPFAEVCWYFTDTWEQFRISGRMEVVDASCLDATKFKQRAEAWSSISP 120

Query: 138 KSRMQYLGPSPNLPYIAEQPAKEPVLDSCSGPVDAFCLLVLDPDQVDYLNLKSAERKLFK 197
           KSRMQYLGPSP LP++AEQPAKEP LDS SGPVDAFCLLV DPDQVDYLNLKS ER LF+
Sbjct: 121 KSRMQYLGPSPGLPHLAEQPAKEPFLDSYSGPVDAFCLLVFDPDQVDYLNLKSEERNLFR 180

Query: 198 AIPCVGRETLWESERINP 216
           AIP  G E LWE+ERINP
Sbjct: 181 AIPSFGGEKLWETERINP 198

[Close]

BLAST of Carg25704 vs. TAIR10
Match: AT2G46580.1 (Pyridoxamine 5'-phosphate oxidase family protein)

HSP 1 Score: 239.2 bits (609), Expect = 2.4e-63
Identity = 111/198 (56.06%), Postives = 150/198 (75.76%), Query Frame = 0


Query: 18  MGSAAAPWKSLLLSALESNAQIKHSTFLHLATIGANGRPSNRTVVFRGFEEGTDRIHIYT 77
           MG+  APWK LL  A+E+N+ + HS+++ LATIG NGRPSNRTVVFRGFEE +DRI I T
Sbjct: 1   MGTHVAPWKQLLFGAIEANSHLSHSSYVQLATIGLNGRPSNRTVVFRGFEENSDRIQINT 60

Query: 78  DSRSRKIEELKTCPFAEISWYFTESWEQFRISGRIEVVDASCLDATKFKKRAEAWSSISP 137
           D RSRKIEELK CPF+E+ WYF+++WEQFRI+GRIEV+DAS  D TK ++R +AW + S 
Sbjct: 61  DLRSRKIEELKHCPFSEMCWYFSDTWEQFRINGRIEVIDASNPDQTKLQQREKAWFANSL 120

Query: 138 KSRMQYLGPSPNLPYIAEQPAKEPVLDSCSGPVDAFCLLVLDPDQVDYLNLKSAERKLFK 197
           +SR+ Y+ P+P  P  +EQ +++  LD  SGPV  +CLL+L+P++VDYLNLK+ +R  F 
Sbjct: 121 RSRLIYVCPTPGSPCNSEQSSQQVKLDPSSGPVPEYCLLLLEPEKVDYLNLKTNQRLFFS 180

Query: 198 AIPCVGRETLWESERINP 216
           ++     E  W SE++NP
Sbjct: 181 SMATGTGEKCWTSEKVNP 198

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BLAST of Carg25704 vs. Swiss-Prot
Match: sp|Q9ZPY1|PPOX2_ARATH (Pyridoxine/pyridoxamine 5'-phosphate oxidase 2 OS=Arabidopsis thaliana OX=3702 GN=PPOX2 PE=1 SV=2)

HSP 1 Score: 239.2 bits (609), Expect = 4.3e-62
Identity = 111/198 (56.06%), Postives = 150/198 (75.76%), Query Frame = 0


Query: 18  MGSAAAPWKSLLLSALESNAQIKHSTFLHLATIGANGRPSNRTVVFRGFEEGTDRIHIYT 77
           MG+  APWK LL  A+E+N+ + HS+++ LATIG NGRPSNRTVVFRGFEE +DRI I T
Sbjct: 1   MGTHVAPWKQLLFGAIEANSHLSHSSYVQLATIGLNGRPSNRTVVFRGFEENSDRIQINT 60

Query: 78  DSRSRKIEELKTCPFAEISWYFTESWEQFRISGRIEVVDASCLDATKFKKRAEAWSSISP 137
           D RSRKIEELK CPF+E+ WYF+++WEQFRI+GRIEV+DAS  D TK ++R +AW + S 
Sbjct: 61  DLRSRKIEELKHCPFSEMCWYFSDTWEQFRINGRIEVIDASNPDQTKLQQREKAWFANSL 120

Query: 138 KSRMQYLGPSPNLPYIAEQPAKEPVLDSCSGPVDAFCLLVLDPDQVDYLNLKSAERKLFK 197
           +SR+ Y+ P+P  P  +EQ +++  LD  SGPV  +CLL+L+P++VDYLNLK+ +R  F 
Sbjct: 121 RSRLIYVCPTPGSPCNSEQSSQQVKLDPSSGPVPEYCLLLLEPEKVDYLNLKTNQRLFFS 180

Query: 198 AIPCVGRETLWESERINP 216
           ++     E  W SE++NP
Sbjct: 181 SMATGTGEKCWTSEKVNP 198

[Close]

BLAST of Carg25704 vs. Swiss-Prot
Match: sp|Q7P203|PDXH_CHRVO (Pyridoxine/pyridoxamine 5'-phosphate oxidase OS=Chromobacterium violaceum (strain ATCC 12472 / DSM 30191 / JCM 1249 / NBRC 12614 / NCIMB 9131 / NCTC 9757) OX=243365 GN=pdxH PE=3 SV=1)

HSP 1 Score: 63.9 bits (154), Expect = 2.5e-09
Identity = 36/102 (35.29%), Postives = 59/102 (57.84%), Query Frame = 0


Query: 37  AQIKHSTFLHLATIGANGRPSNRTVVFRGFEEGTDRIHIYTDSRSRKIEELKTCPFAEIS 96
           AQ+   T ++LA IGA+GRPS+R V+ +G E+G  ++  YT+ +SRK + L+  P+  ++
Sbjct: 40  AQVPEPTAMNLAAIGADGRPSSRIVLLKGVEDG--QLLFYTNYQSRKGQALEANPYVALN 99

Query: 97  WYFTESWEQFRISGRIEVVDASCLDA----TKFKKRAEAWSS 135
           +++ E   Q RI G+   V     DA      +  R  AW+S
Sbjct: 100 FFWPELERQVRIEGKAARVAPEVSDAYFASRPYTSRLGAWAS 139

[Close]

BLAST of Carg25704 vs. Swiss-Prot
Match: sp|Q65QT9|PDXH_MANSM (Pyridoxine/pyridoxamine 5'-phosphate oxidase OS=Mannheimia succiniciproducens (strain MBEL55E) OX=221988 GN=pdxH PE=3 SV=1)

HSP 1 Score: 57.0 bits (136), Expect = 3.0e-07
Identity = 30/102 (29.41%), Postives = 54/102 (52.94%), Query Frame = 0


Query: 37  AQIKHSTFLHLATIGANGRPSNRTVVFRGFEEGTDRIHIYTDSRSRKIEELKTCPFAEIS 96
           A++   T +++AT+G NG+PS+R V+ +  E     +  +T+  S K  +L   PFA ++
Sbjct: 39  AKVNEPTAMNVATVGENGKPSSRVVLLK--EVNERGLVFFTNYHSHKGRDLAVNPFAAVN 98

Query: 97  WYFTESWEQFRISGRIEVVDASCLD----ATKFKKRAEAWSS 135
            ++ E   Q R+ GR+E +     D    +  +  R  AW+S
Sbjct: 99  LFWAELQRQVRVEGRVERISPQASDEYFASRPYTSRIGAWAS 138

[Close]

BLAST of Carg25704 vs. Swiss-Prot
Match: sp|A1TQY3|PDXH_ACIAC (Pyridoxine/pyridoxamine 5'-phosphate oxidase OS=Acidovorax citrulli (strain AAC00-1) OX=397945 GN=pdxH PE=3 SV=1)

HSP 1 Score: 56.6 bits (135), Expect = 3.9e-07
Identity = 37/102 (36.27%), Postives = 54/102 (52.94%), Query Frame = 0


Query: 37  AQIKHSTFLHLATIGANGRPSNRTVVFRGFEEGTDRIHIYTDSRSRKIEELKTCPFAEIS 96
           AQ+     + LAT+GA+ RPS R V+ +G++E    I  YT+  SRK  +L   PFA + 
Sbjct: 52  AQVPEPNAMTLATVGADLRPSTRVVLIKGYDE--RGIVWYTNYGSRKGRQLAGNPFAALQ 111

Query: 97  WYFTESWEQFRISGRIEVVDASCLDA----TKFKKRAEAWSS 135
           +++ E     RI GR+E V  +  DA         R  AW+S
Sbjct: 112 FHWVELERVVRIEGRVEKVSDAESDAYFASRPLDSRIGAWAS 151

[Close]

BLAST of Carg25704 vs. Swiss-Prot
Match: sp|A9AGI1|PDXH_BURM1 (Pyridoxine/pyridoxamine 5'-phosphate oxidase OS=Burkholderia multivorans (strain ATCC 17616 / 249) OX=395019 GN=pdxH PE=3 SV=1)

HSP 1 Score: 55.8 bits (133), Expect = 6.7e-07
Identity = 35/102 (34.31%), Postives = 53/102 (51.96%), Query Frame = 0


Query: 37  AQIKHSTFLHLATIGANGRPSNRTVVFRGFEEGTDRIHIYTDSRSRKIEELKTCPFAEIS 96
           A++     + LAT+GA+GRPS R V+ +G +E       +T+  SRK  +L   P+A + 
Sbjct: 39  AKLPEPNTMTLATVGADGRPSARIVLVKGVDE--RGFVFFTNYESRKGRDLAAHPYAALL 98

Query: 97  WYFTESWEQFRISGRIEVVDASCLD----ATKFKKRAEAWSS 135
           +Y+ E   Q RI GR+E   A   D    +     R  AW+S
Sbjct: 99  FYWIELERQVRIEGRVEKTSAEESDRYFASRPVGSRIGAWAS 138

[Close]

BLAST of Carg25704 vs. TrEMBL
Match: tr|A0A1S4DSQ7|A0A1S4DSQ7_CUCME (pyridoxine/pyridoxamine 5'-phosphate oxidase 2 OS=Cucumis melo OX=3656 GN=LOC103483173 PE=4 SV=1)

HSP 1 Score: 354.4 bits (908), Expect = 1.8e-94
Identity = 170/198 (85.86%), Postives = 179/198 (90.40%), Query Frame = 0


Query: 18  MGSAAAPWKSLLLSALESNAQIKHSTFLHLATIGANGRPSNRTVVFRGFEEGTDRIHIYT 77
           MGSA APWKSLLL ALESN QIKHS FL LATIG NGRPSNRTVVFR FEEGTDRIHIYT
Sbjct: 1   MGSAVAPWKSLLLRALESNGQIKHSKFLQLATIGTNGRPSNRTVVFREFEEGTDRIHIYT 60

Query: 78  DSRSRKIEELKTCPFAEISWYFTESWEQFRISGRIEVVDASCLDATKFKKRAEAWSSISP 137
           DSRSRKIEELK CPFAE+ WYFT++WEQFRISGR+EVVDASCLDATKFK+RAEAWSSISP
Sbjct: 61  DSRSRKIEELKNCPFAEVCWYFTDTWEQFRISGRMEVVDASCLDATKFKQRAEAWSSISP 120

Query: 138 KSRMQYLGPSPNLPYIAEQPAKEPVLDSCSGPVDAFCLLVLDPDQVDYLNLKSAERKLFK 197
           KSRMQYLGPSP LP++AEQPAKEP LDS SGPVDAFCLLV DPDQVDYLNLKS ER LF+
Sbjct: 121 KSRMQYLGPSPGLPHLAEQPAKEPFLDSYSGPVDAFCLLVFDPDQVDYLNLKSEERNLFR 180

Query: 198 AIPCVGRETLWESERINP 216
           AIP  G E LWE+ERINP
Sbjct: 181 AIPSFGGEKLWETERINP 198

[Close]

BLAST of Carg25704 vs. TrEMBL
Match: tr|A0A0A0L745|A0A0A0L745_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G119450 PE=4 SV=1)

HSP 1 Score: 349.4 bits (895), Expect = 5.9e-93
Identity = 167/197 (84.77%), Postives = 176/197 (89.34%), Query Frame = 0


Query: 18  MGSAAAPWKSLLLSALESNAQIKHSTFLHLATIGANGRPSNRTVVFRGFEEGTDRIHIYT 77
           MGSA APWKSLLLSALESN   KHS FL LATIG NGRPSNRTVVFRGFEEGTDRIHIYT
Sbjct: 1   MGSAVAPWKSLLLSALESNGHFKHSKFLQLATIGTNGRPSNRTVVFRGFEEGTDRIHIYT 60

Query: 78  DSRSRKIEELKTCPFAEISWYFTESWEQFRISGRIEVVDASCLDATKFKKRAEAWSSISP 137
           DSR+RKIEELK CPFAEI WYFTESWEQFRISGR+EVVDASC+DATKFK+RAEAWSSISP
Sbjct: 61  DSRNRKIEELKNCPFAEICWYFTESWEQFRISGRMEVVDASCVDATKFKQRAEAWSSISP 120

Query: 138 KSRMQYLGPSPNLPYIAEQPAKEPVLDSCSGPVDAFCLLVLDPDQVDYLNLKSAERKLFK 197
           KSRMQYLGPSP LP++AEQPAKEP LDSCSGPVD FCLLV DPDQVDYLN++S ER  FK
Sbjct: 121 KSRMQYLGPSPGLPHLAEQPAKEPFLDSCSGPVDTFCLLVFDPDQVDYLNVRSDERNSFK 180

Query: 198 AIPCVGRETLWESERIN 215
           AI   G E LWE+ERIN
Sbjct: 181 AITSFGGEKLWETERIN 197

[Close]

BLAST of Carg25704 vs. TrEMBL
Match: tr|A0A2R6RCG8|A0A2R6RCG8_ACTCH (Pyridoxine/pyridoxamine 5'-phosphate oxidase OS=Actinidia chinensis var. chinensis OX=1590841 GN=CEY00_Acc07562 PE=4 SV=1)

HSP 1 Score: 263.8 bits (673), Expect = 3.3e-67
Identity = 124/198 (62.63%), Postives = 154/198 (77.78%), Query Frame = 0


Query: 18  MGSAAAPWKSLLLSALESNAQIKHSTFLHLATIGANGRPSNRTVVFRGFEEGTDRIHIYT 77
           MG+ AAPWK LLL+ALESN+ +KHS+F  LATIG+NG+PSNRTVVFRGF+E +D+I I T
Sbjct: 1   MGTVAAPWKQLLLNALESNSTLKHSSFFQLATIGSNGKPSNRTVVFRGFQENSDKIQINT 60

Query: 78  DSRSRKIEELKTCPFAEISWYFTESWEQFRISGRIEVVDASCLDATKFKKRAEAWSSISP 137
           D R+ KI++LK CPFAEI WYFT+SWEQFRI+GRI+V+  SC D  K ++R ++W + S 
Sbjct: 61  DYRTHKIDDLKHCPFAEICWYFTDSWEQFRINGRIDVIYESCPDPMKLQQREKSWFASSL 120

Query: 138 KSRMQYLGPSPNLPYIAEQPAKEPVLDSCSGPVDAFCLLVLDPDQVDYLNLKSAERKLFK 197
           KSR+QYLGP+P LP + E P++E  LD  +GPV+AFCLLVLDPDQVDYLNLK  ER  F 
Sbjct: 121 KSRLQYLGPNPGLPLLGELPSQESSLDPSAGPVEAFCLLVLDPDQVDYLNLKINERLTFT 180

Query: 198 AIPCVGRETLWESERINP 216
           +      E  W SERINP
Sbjct: 181 SRLSTDGEKFWTSERINP 198

[Close]

BLAST of Carg25704 vs. TrEMBL
Match: tr|A0A061DTI4|A0A061DTI4_THECC (Pyridoxamine 5'-phosphate oxidase family protein isoform 1 OS=Theobroma cacao OX=3641 GN=TCM_005113 PE=4 SV=1)

HSP 1 Score: 262.3 bits (669), Expect = 9.5e-67
Identity = 127/198 (64.14%), Postives = 151/198 (76.26%), Query Frame = 0


Query: 18  MGSAAAPWKSLLLSALESNAQIKHSTFLHLATIGANGRPSNRTVVFRGFEEGTDRIHIYT 77
           MG+  APWK LLL+ALESN+ IKHS+F  LATIG+NGRPSNRTVVFRGF+E +D+  I T
Sbjct: 1   MGTVTAPWKQLLLNALESNSHIKHSSFFQLATIGSNGRPSNRTVVFRGFQENSDKFQINT 60

Query: 78  DSRSRKIEELKTCPFAEISWYFTESWEQFRISGRIEVVDASCLDATKFKKRAEAWSSISP 137
           DSRS KIEELK CPFAEI WYFT+SWEQFR SGRI+V+D S  +A K ++R ++W + S 
Sbjct: 61  DSRSHKIEELKHCPFAEICWYFTDSWEQFRFSGRIDVIDGSNPEALKLQQREKSWFASSV 120

Query: 138 KSRMQYLGPSPNLPYIAEQPAKEPVLDSCSGPVDAFCLLVLDPDQVDYLNLKSAERKLFK 197
           KSR+QYLGP P  P I+E P +E  LD  +GPV AFCLLVLDPDQVDYLNLKS +R  F 
Sbjct: 121 KSRLQYLGPIPGFPCISEHPNQEIALDPSTGPVGAFCLLVLDPDQVDYLNLKSNQRLKFI 180

Query: 198 AIPCVGRETLWESERINP 216
           +   +     W SERINP
Sbjct: 181 STLGLNGVKSWTSERINP 198

[Close]

BLAST of Carg25704 vs. TrEMBL
Match: tr|A0A2I0ILT0|A0A2I0ILT0_PUNGR (Uncharacterized protein OS=Punica granatum OX=22663 GN=CRG98_034654 PE=4 SV=1)

HSP 1 Score: 262.3 bits (669), Expect = 9.5e-67
Identity = 125/196 (63.78%), Postives = 153/196 (78.06%), Query Frame = 0


Query: 20  SAAAPWKSLLLSALESNAQIKHSTFLHLATIGANGRPSNRTVVFRGFEEGTDRIHIYTDS 79
           +A APWK LLL+ALESN+ +KHS++L LATIG+NGRPSNRTVVFRGFEE +D+I I+TD 
Sbjct: 5   TATAPWKQLLLNALESNSHLKHSSYLQLATIGSNGRPSNRTVVFRGFEENSDKIQIHTDR 64

Query: 80  RSRKIEELKTCPFAEISWYFTESWEQFRISGRIEVVDASCLDATKFKKRAEAWSSISPKS 139
           RSRKIEEL+ CPFAEI WYFT+SWEQFRI+GR+EV+D    D+ K ++R +AW S S +S
Sbjct: 65  RSRKIEELRHCPFAEICWYFTDSWEQFRINGRVEVIDEMNPDSLKREQREKAWFSCSMRS 124

Query: 140 RMQYLGPSPNLPYIAEQPAKEPVLDSCSGPVDAFCLLVLDPDQVDYLNLKSAERKLFKAI 199
           R+QYLGPSP +P + E    E  +D  +GPV AFCLLVL+PDQVDYLNLKS +R  F   
Sbjct: 125 RLQYLGPSPGVPRLNEDFPAEASVDPSTGPVGAFCLLVLEPDQVDYLNLKSNQRLEFTFT 184

Query: 200 PCVGRETLWESERINP 216
              G+E  W SERINP
Sbjct: 185 RTAGKEKCWTSERINP 200

The following BLAST results are available for this feature:

BLAST of Carg25704 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022924710.1	2.3e-120	100.00	pyridoxine/pyridoxamine 5'-phosphate oxidase 2 isoform X1 [Cucurbita moschata]	[more]
XP_022980848.1	1.4e-106	96.97	pyridoxine/pyridoxamine 5'-phosphate oxidase 2 isoform X1 [Cucurbita maxima]	[more]
XP_023527386.1	2.7e-105	95.96	pyridoxine/pyridoxamine 5'-phosphate oxidase 2 isoform X1 [Cucurbita pepo subsp....	[more]
XP_022924711.1	2.5e-95	85.12	pyridoxine/pyridoxamine 5'-phosphate oxidase 2 isoform X2 [Cucurbita moschata]	[more]
XP_008437871.1	2.8e-94	85.86	PREDICTED: pyridoxine/pyridoxamine 5'-phosphate oxidase 2 [Cucumis melo] >XP_016...	[more]

BLAST of Carg25704 vs. TAIR10


Match Name	E-value	Identity	Description
AT2G46580.1	2.4e-63	56.06	Pyridoxamine 5'-phosphate oxidase family protein	[more]

BLAST of Carg25704 vs. Swiss-Prot


Match Name	E-value	Identity	Description
sp\|Q9ZPY1\|PPOX2_ARATH	4.3e-62	56.06	Pyridoxine/pyridoxamine 5'-phosphate oxidase 2 OS=Arabidopsis thaliana OX=3702 G...	[more]
sp\|Q7P203\|PDXH_CHRVO	2.5e-09	35.29	Pyridoxine/pyridoxamine 5'-phosphate oxidase OS=Chromobacterium violaceum (strai...	[more]
sp\|Q65QT9\|PDXH_MANSM	3.0e-07	29.41	Pyridoxine/pyridoxamine 5'-phosphate oxidase OS=Mannheimia succiniciproducens (s...	[more]
sp\|A1TQY3\|PDXH_ACIAC	3.9e-07	36.27	Pyridoxine/pyridoxamine 5'-phosphate oxidase OS=Acidovorax citrulli (strain AAC0...	[more]
sp\|A9AGI1\|PDXH_BURM1	6.7e-07	34.31	Pyridoxine/pyridoxamine 5'-phosphate oxidase OS=Burkholderia multivorans (strain...	[more]

BLAST of Carg25704 vs. TrEMBL


Match Name	E-value	Identity	Description
tr\|A0A1S4DSQ7\|A0A1S4DSQ7_CUCME	1.8e-94	85.86	pyridoxine/pyridoxamine 5'-phosphate oxidase 2 OS=Cucumis melo OX=3656 GN=LOC103...	[more]
tr\|A0A0A0L745\|A0A0A0L745_CUCSA	5.9e-93	84.77	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G119450 PE=4 SV=1	[more]
tr\|A0A2R6RCG8\|A0A2R6RCG8_ACTCH	3.3e-67	62.63	Pyridoxine/pyridoxamine 5'-phosphate oxidase OS=Actinidia chinensis var. chinens...	[more]
tr\|A0A061DTI4\|A0A061DTI4_THECC	9.5e-67	64.14	Pyridoxamine 5'-phosphate oxidase family protein isoform 1 OS=Theobroma cacao OX...	[more]
tr\|A0A2I0ILT0\|A0A2I0ILT0_PUNGR	9.5e-67	63.78	Uncharacterized protein OS=Punica granatum OX=22663 GN=CRG98_034654 PE=4 SV=1	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **Molecular Function**
Term	Definition
GO:0048037	cofactor binding
GO:0010181	FMN binding

Vocabulary: **INTERPRO**
Term	Definition
IPR012349	Split_barrel_FMN-bd
IPR024015	Pyridox_Oxase_FMN-dep_Alr4036
IPR024624	Pyridox_Oxase_Alr4036_FMN-bd

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0008150	biological_process
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0010181	FMN binding
molecular_function	GO:0048037	cofactor binding

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg25704-RA	Carg25704-RA	mRNA

InterPro

Analysis Name: InterPro Annotations of silver-seed gourd
Date Performed: 2019-03-07


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR024624	Pyridoxamine 5'-phosphate oxidase, Alr4036 family, FMN-binding domain	PFAM	PF12766	Pyridox_oxase_2	coord: 23..115 e-value: 8.7E-27 score: 93.6
IPR024015	Pyridoxamine 5'-phosphate oxidase, probable FMN-dependent, Alr4036 family	TIGRFAM	TIGR04026	TIGR04026	coord: 23..215 e-value: 1.4E-47 score: 160.0
IPR012349	FMN-binding split barrel	GENE3D	G3DSA:2.30.110.10		coord: 20..215 e-value: 4.3E-56 score: 191.8
None	No IPR available	PANTHER	PTHR10851	PYRIDOXINE-5-PHOSPHATE OXIDASE	coord: 18..215
None	No IPR available	PANTHER	PTHR10851:SF3	SUBFAMILY NOT NAMED	coord: 18..215
None	No IPR available	SUPERFAMILY	SSF50475	FMN-binding split barrel	coord: 18..215

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Carg25704	Cla021143	Watermelon (97103) v1	carwmB0122
Carg25704	Csa3G119450	Cucumber (Chinese Long) v2	carcuB0132
Carg25704	MELO3C006011	Melon (DHL92) v3.5.1	carmeB0128
Carg25704	ClCG05G000710	Watermelon (Charleston Gray)	carwcgB0122
Carg25704	CSPI03G07810	Wild cucumber (PI 183967)	carcpiB0137
Carg25704	Cucsa.259350	Cucumber (Gy14) v1	carcgyB0148
Carg25704	CmaCh14G021190	Cucurbita maxima (Rimu)	carcmaB0162
Carg25704	CmoCh14G021800	Cucurbita moschata (Rifu)	carcmoB0170
Carg25704	Lsi05G021350	Bottle gourd (USVL1VR-Ls)	carlsiB110
Carg25704	Cp4.1LG03g15960	Cucurbita pepo (Zucchini)	carcpeB0179
Carg25704	CsGy3G007960	Cucumber (Gy14) v2	carcgybB0131
Carg25704	MELO3C006011.2	Melon (DHL92) v3.6.1	carmedB0132
Carg25704	CsaV3_3G007930	Cucumber (Chinese Long) v3	carcucB0141
Carg25704	Cla97C05G080820	Watermelon (97103) v2	carwmbB0130
Carg25704	Bhi01G000367	Wax gourd	carwgoB0153

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Carg25704	Wax gourd	carwgoB0152
Carg25704	Silver-seed gourd	carcarB146
Carg25704	Cucurbita maxima (Rimu)	carcmaB0164
Carg25704	Cucurbita moschata (Rifu)	carcmoB0171
Carg25704	Cucurbita pepo (Zucchini)	carcpeB0180