Cp4.1LG01g01850 (gene) Cucurbita pepo (Zucchini)

NameCp4.1LG01g01850
Typegene
OrganismCucurbita pepo (Cucurbita pepo (Zucchini))
DescriptionSerine hydroxymethyltransferase
LocationCp4.1LG01 : 2758138 .. 2762234 (-)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGATTTGTCTAAGTCCCATTCCAATCTTTCTCTTGGATTCTCATCGCATGCTTCCCCGCCTCACCGTGCTCAGATTGCTGATGATTCCGTCCTGCTGCATCTCGATTCTTCTCGACGGGAATCCTCGTATTCGTCCACCCCAGTCCCTCTTCAGTTGATGGAACAGCAGACGGAGAGTCACAGAAATGATGGTGGAGATGGGGAGAGAGATGACGATGAATACGACGACAGGGAGGTAGAAGAGTTTCGGATTCTGGGTCATCCTATGTGTTTGAAGCGTCGAAGAGATGGCGAATCTTCGACATCCTCCGGTTCTGCGAAAAGAATTTCGTGTGAATTTGATATAGAGACACGAAGGAGTGCTGTACGGTCATGGGGTGATCAGACCCTTCGAGTTGCGGACCCTAATCTATTTGGGATTATGGAGAAGGAGAAGCAGAGGCAATTCAAGGGCATTGAATTGATTGCTTCTGAAAATTATGTGTGTCGGGCTGTAATGGAAGCATTGGGAAGCCATTTGACTAATAAGTACTCGGAAGGTATGCCCGGTGCGAGGTATTACTGTGGAAATCAATATATAGATGAGATTGAAACCCTATGTCGTGAGCGCGCATTGGCGGCATTTGGTCTTAGTTCTGATAATTGGGGAGTTAACGTGCAACCTTATTCATGCACATCTGCGAACTTTGCTGTCTACACTGGTCTGTTATTGCCGGGCGATCGGATCATGGGGTTGGATACTCCTTCTGGTGGTAATACAAGCCATGGGTACTGTACCCCAAATGGAAGAAAAGTATCTGGGGCCTCAATTTTCTTTGAGAGTTTGCCATATAAGGTGAATCCACAAACTGGTTATATCGATTATGATAAGCTTGAGGAGCGGGCACTTGACTTTCGCCCAAAAATACTTATCTGTGGTGGGAGCTCGTATCCTCGTGAACTGGATTATGCAAGGTTTAGGCAGATTGCAGATAAATGTGGAGCAGTTCTGATGTGTGATATGGCTCAAATTAGTGGCCTTGTTGCTGCCAAGGTGTGATTTTTTCTCCATCTTTAGCGATGATACGACCCTTGAATATCTGTGTATATATCTTTTGGTTTTTCTTATCATATGTACTAGTTACTATCCTGTCTTCATTCTCTTGGATCTCTATTTAGTTGGTTGTTAATATAATCAAGATATTTGAATTGTTTTACCTGACATTGAGACATGTCTTCAAAGGTGGCTGTGTGGGGACTTCATTGTGGTTTTTGGTATTTAGGATATGTATGGCTTTGGCTATGAAACTTAGTGATACGAAAATGTAATTGATTGAAGTATTTTGAGACAAGACGTTTTGGTACATTGGCCAATCGTGAATGCTCTGAATCTTTAAGCTTATGACTGTGGCATTGTTTTTCTTTTCTACTTTTATTAAAGATAGTTTTTGTTTCTACGTGCTTCTATTCTTCATTTTCATTCAACAGTGTATTTATGCATTTTTCAGTTTTTTTTAGGTCTTCGTGTTATAACAATGCCTTGAATATATATATTTTTTAATAACCCGATATGGGGTCTCCTGTGCCTGCCCCAAGATCCGGACCAGGAGCCTTTTCAATTTGTTGAACCTTTTTTTGTTACTTTTTTTTTTTTTTATGGTGGAGTGCATGTTTTGACACCCGTTTCGTAGTCACTAAAGCTTGCACTTTCGTTACTCGGTGAATTGTTACTTTTTTTTTTTTACGAAAATAGTATGTTGCTTTGACATCTGTTTTTTTTTTTTTTTTCTACTTATATTGTTTGATCCATCAGTACTTCTTATGTGTAATCTTGTAGTTTTAGATGGAAAATTATCCCTACTCTTCCGTTCATATTTTGATTTTGGTTTGAATTTTCTGACAGGAATGTGTCAGCCCTTTTGGGTACTGTGATGTTGTGACTTCAACTACTCATAAAAGTCTTCGAGGTCCAAGGGGAGGTATAATTTTTTATAGGAAGGGTAGTAAGCCGAGGAAGAGAGCAATGCTTCTGTATCAGGGAAATGAAACTGACCAATATGATTTTGAGGAGAAGATAAACTTTGCAGTTTTTCCCTCCTTACAAGGTGGACCTCACAATAACCATATTGCTGCTCTTGCCGTGGCCCTAAAGCAAGTGGCTACTCCCGAGTACAAGGCATATATGCAGCAGGTGAAAAAAAATGCTCAAGCCTTGGCTGCTGCTTTACTTCGAAAGAAGTACAAGCTTGTAACCGGTGGCACAGATAACCACATGATACTCTGGGATCTAAGGTCCTTGGGATTGACAGGTGCATAATTTTGACTTGAATGTTGCAAACTGATGTGAACATGATATATTTAAGGTGCACAACTAGCTTTTTGTTTGTCTAGGGGGAAGTGGCACACAAAAAACGTTGCTCAAGCATAGTGAGGGTTGATTAAGTTATGCACTGTGGGTTATGATTAGGGGAAATGGCATATTATATTATTATTGCATAACATGATTTACATAATTTGGAATAAGTATATATCTCTTATATCAACTGAATTGCCAGACGAAATAGGATTTTTTTTTTCTTTTTATACTTAAATTAGCTTAAAAACTACTTGCTTCGGCACAAATATGATTTTTATTTAGCAATATCTGTTGCTTTTTTTCTTTTTCATTTCATAGATTATGTATGACTTTTCATTTCATAGGTTATGTATGACTTTTCATTTCATAGGTTATGTATGACTTTTCATTTCATAGATTATGCCTGTGCATTTAATTACTCTAATAAGAAAGCTGAATACATTCTTTAATAAGGTGTTAAAAGTGATGAAACTGTGAACAAGGCATGCAACTGTTAGTCATGATAAGTCTTAGCATAGCCTTCAAAACTCACCAAAACTATGGAAAAGTACAGAAAGGGTGATGAGAAATCAACCCAAAAAGGCCTTTAGGAATTGGATAAGTTTCTCCAATTGGCAGCGACTAATAATATTGTAGGTACTAAAAAAAAAATAAAAAAAAAATAAAAAAGGTTTTTTTAAGGAACTCCAAGAAGCCTTGTATTCAATGAAGATGAACTCTAAACTTCTGAAATAATTTATGATTTCATTCTAGTCGAACTTCCACAAGTAAAGATTCTTTGCTTTGTACTCTAAGGGATGCCTCCTTGGCAAACAATCTAGACTTTGCAGAACAGAAAAGGGTAGGCATTAAGCAAAATTAAACTTATCTAGCAGAGTTCCTCAAAGATTTCTGTAGCAGTATAGGAATAGACTGTTTTTGGCTTTTGAACCATCTTTATCTTGAGCAGCACAGCTATAAGAGATGAGTCTGGGGATTTCTTCTTTGTGTAATATCAGGCATATTGATGCCCGTTATCACTAAGTTTCACAGAACTTCTTGTCTTCTTAGGGCTTCCTTTCTGCCAAATGACTCCAAAAGTCTCTTGTTAAGAGTGGTGTAAAATCTATTACAACGCACAACTTGTTTTTTGTTCATATTTGTATGCGTCTATTTCTTCTGAGTCCTATTTTTGATGGTATCTGTGGTGGCTTAGTCTATTATTCTACATTTATATTGTGTAACTTACTGATGGCATGGTAATTGTAGGTAAAAACTATGAGAATGTTTGTGAGATCTGCCACATTACTCTCAACAAAATTACAATATTTGGTGATAATGGAATTATCATACCCGGAGGAGTACGTGTTGGTGAGTAATGCCCTCTTATATTTCATATTTTGGGCTCCTTTTATTCGAGTTTTTTTTTGTATGCCTTTTTATTTTATTCATTTTCTCAATGAAAACTTAGTTTCTTACCGAAAGAAATATCTTGGGGTATCCTATGTTTGGAGAGCTTAGTATGCGTTATAAATTGGCTCACTGTTATCCCTGCAGGCACCCCAGCTATGACATCAAGAGGCTGTCTGGAGTCTGATTTTGAGACAATTGCTGATTTTCTTCATACAGCCGCTCAGATTGCAATTTCAGTACAGCGAGAGTATGGGAAGCTACCAAAGACTTATCTCAAGGGCCTTCTAAGTAATAATGAAGTGGTTGAGCTCCGAAACCGCGTTGAAAGTTTTTCAGCTCAATTTGCAATGCCAGGCTTTGAAATTTGA

mRNA sequence

ATGGATTTGTCTAAGTCCCATTCCAATCTTTCTCTTGGATTCTCATCGCATGCTTCCCCGCCTCACCGTGCTCAGATTGCTGATGATTCCGTCCTGCTGCATCTCGATTCTTCTCGACGGGAATCCTCGTATTCGTCCACCCCAGTCCCTCTTCAGTTGATGGAACAGCAGACGGAGAGTCACAGAAATGATGGTGGAGATGGGGAGAGAGATGACGATGAATACGACGACAGGGAGGTAGAAGAGTTTCGGATTCTGGGTCATCCTATGTGTTTGAAGCGTCGAAGAGATGGCGAATCTTCGACATCCTCCGGTTCTGCGAAAAGAATTTCGTGTGAATTTGATATAGAGACACGAAGGAGTGCTGTACGGTCATGGGGTGATCAGACCCTTCGAGTTGCGGACCCTAATCTATTTGGGATTATGGAGAAGGAGAAGCAGAGGCAATTCAAGGGCATTGAATTGATTGCTTCTGAAAATTATGTGTGTCGGGCTGTAATGGAAGCATTGGGAAGCCATTTGACTAATAAGTACTCGGAAGGTATGCCCGGTGCGAGGTATTACTGTGGAAATCAATATATAGATGAGATTGAAACCCTATGTCGTGAGCGCGCATTGGCGGCATTTGGTCTTAGTTCTGATAATTGGGGAGTTAACGTGCAACCTTATTCATGCACATCTGCGAACTTTGCTGTCTACACTGGTCTGTTATTGCCGGGCGATCGGATCATGGGGTTGGATACTCCTTCTGGTGGTAATACAAGCCATGGGTACTGTACCCCAAATGGAAGAAAAGTATCTGGGGCCTCAATTTTCTTTGAGAGTTTGCCATATAAGGAATGTGTCAGCCCTTTTGGGTACTGTGATGTTGTGACTTCAACTACTCATAAAAGTCTTCGAGGTCCAAGGGGAGGTATAATTTTTTATAGGAAGGGTAGTAAGCCGAGGAAGAGAGCAATGCTTCTGTATCAGGGAAATGAAACTGACCAATATGATTTTGAGGAGAAGATAAACTTTGCAGTTTTTCCCTCCTTACAAGGTGGACCTCACAATAACCATATTGCTGCTCTTGCCGTGGCCCTAAAGCAAGTGGCTACTCCCGAGTACAAGGCATATATGCAGCAGGTGAAAAAAAATGCTCAAGCCTTGGCTGCTGCTTTACTTCGAAAGAAGTACAAGCTTGTAACCGGTGGCACAGATAACCACATGATACTCTGGGATCTAAGGTCCTTGGGATTGACAGGTAAAAACTATGAGAATGTTTGTGAGATCTGCCACATTACTCTCAACAAAATTACAATATTTGGTGATAATGGAATTATCATACCCGGAGGAGTACGTGTTGCCGCTCAGATTGCAATTTCAGTACAGCGAGAGTATGGGAAGCTACCAAAGACTTATCTCAAGGGCCTTCTAAGTAATAATGAAGTGGTTGAGCTCCGAAACCGCGTTGAAAGTTTTTCAGCTCAATTTGCAATGCCAGGCTTTGAAATTTGA

Coding sequence (CDS)

ATGGATTTGTCTAAGTCCCATTCCAATCTTTCTCTTGGATTCTCATCGCATGCTTCCCCGCCTCACCGTGCTCAGATTGCTGATGATTCCGTCCTGCTGCATCTCGATTCTTCTCGACGGGAATCCTCGTATTCGTCCACCCCAGTCCCTCTTCAGTTGATGGAACAGCAGACGGAGAGTCACAGAAATGATGGTGGAGATGGGGAGAGAGATGACGATGAATACGACGACAGGGAGGTAGAAGAGTTTCGGATTCTGGGTCATCCTATGTGTTTGAAGCGTCGAAGAGATGGCGAATCTTCGACATCCTCCGGTTCTGCGAAAAGAATTTCGTGTGAATTTGATATAGAGACACGAAGGAGTGCTGTACGGTCATGGGGTGATCAGACCCTTCGAGTTGCGGACCCTAATCTATTTGGGATTATGGAGAAGGAGAAGCAGAGGCAATTCAAGGGCATTGAATTGATTGCTTCTGAAAATTATGTGTGTCGGGCTGTAATGGAAGCATTGGGAAGCCATTTGACTAATAAGTACTCGGAAGGTATGCCCGGTGCGAGGTATTACTGTGGAAATCAATATATAGATGAGATTGAAACCCTATGTCGTGAGCGCGCATTGGCGGCATTTGGTCTTAGTTCTGATAATTGGGGAGTTAACGTGCAACCTTATTCATGCACATCTGCGAACTTTGCTGTCTACACTGGTCTGTTATTGCCGGGCGATCGGATCATGGGGTTGGATACTCCTTCTGGTGGTAATACAAGCCATGGGTACTGTACCCCAAATGGAAGAAAAGTATCTGGGGCCTCAATTTTCTTTGAGAGTTTGCCATATAAGGAATGTGTCAGCCCTTTTGGGTACTGTGATGTTGTGACTTCAACTACTCATAAAAGTCTTCGAGGTCCAAGGGGAGGTATAATTTTTTATAGGAAGGGTAGTAAGCCGAGGAAGAGAGCAATGCTTCTGTATCAGGGAAATGAAACTGACCAATATGATTTTGAGGAGAAGATAAACTTTGCAGTTTTTCCCTCCTTACAAGGTGGACCTCACAATAACCATATTGCTGCTCTTGCCGTGGCCCTAAAGCAAGTGGCTACTCCCGAGTACAAGGCATATATGCAGCAGGTGAAAAAAAATGCTCAAGCCTTGGCTGCTGCTTTACTTCGAAAGAAGTACAAGCTTGTAACCGGTGGCACAGATAACCACATGATACTCTGGGATCTAAGGTCCTTGGGATTGACAGGTAAAAACTATGAGAATGTTTGTGAGATCTGCCACATTACTCTCAACAAAATTACAATATTTGGTGATAATGGAATTATCATACCCGGAGGAGTACGTGTTGCCGCTCAGATTGCAATTTCAGTACAGCGAGAGTATGGGAAGCTACCAAAGACTTATCTCAAGGGCCTTCTAAGTAATAATGAAGTGGTTGAGCTCCGAAACCGCGTTGAAAGTTTTTCAGCTCAATTTGCAATGCCAGGCTTTGAAATTTGA

Protein sequence

MDLSKSHSNLSLGFSSHASPPHRAQIADDSVLLHLDSSRRESSYSSTPVPLQLMEQQTESHRNDGGDGERDDDEYDDREVEEFRILGHPMCLKRRRDGESSTSSGSAKRISCEFDIETRRSAVRSWGDQTLRVADPNLFGIMEKEKQRQFKGIELIASENYVCRAVMEALGSHLTNKYSEGMPGARYYCGNQYIDEIETLCRERALAAFGLSSDNWGVNVQPYSCTSANFAVYTGLLLPGDRIMGLDTPSGGNTSHGYCTPNGRKVSGASIFFESLPYKECVSPFGYCDVVTSTTHKSLRGPRGGIIFYRKGSKPRKRAMLLYQGNETDQYDFEEKINFAVFPSLQGGPHNNHIAALAVALKQVATPEYKAYMQQVKKNAQALAAALLRKKYKLVTGGTDNHMILWDLRSLGLTGKNYENVCEICHITLNKITIFGDNGIIIPGGVRVAAQIAISVQREYGKLPKTYLKGLLSNNEVVELRNRVESFSAQFAMPGFEI
BLAST of Cp4.1LG01g01850 vs. Swiss-Prot
Match: GLYC7_ARATH (Serine hydroxymethyltransferase 7 OS=Arabidopsis thaliana GN=SHM7 PE=2 SV=1)

HSP 1 Score: 362.5 bits (929), Expect = 7.6e-99
Identity = 196/303 (64.69%), Postives = 227/303 (74.92%), Query Frame = 1

Query: 1   MDLSKSHSNLSLGFS-SHAS----PPHRAQIADDSVLLHLDSSRRESSYSSTPVPLQLME 60
           MDLS+S +N  LGF  SHAS    P  RA IADDS+ L +D S R    + +P+PLQL+E
Sbjct: 1   MDLSRSQTNFQLGFGCSHASMTPTPTPRAPIADDSINLQVDQSFRSLPTTFSPIPLQLLE 60

Query: 61  QQTESH------RNDGGDGERDDDEYDDREVEEFRILGHPMCLKRRRDGESS-TSSGSAK 120
           Q+ E        + DGG G       D +E E FRILGH MCLKR+RD     T S   K
Sbjct: 61  QKAEKTTTVDEPKKDGGGGG------DQKEDEHFRILGHHMCLKRQRDCPLLLTQSKHPK 120

Query: 121 RISC-EFDIETRRSAVRSWGDQTLRVADPNLFGIMEKEKQRQFKGIELIASENYVCRAVM 180
           R S  + D+E+RR+AVR+WGDQ + +ADP++  +MEKEKQRQ +GIELIASEN+VCRAVM
Sbjct: 121 RSSIGDSDLESRRAAVRAWGDQPIHLADPDIHELMEKEKQRQVRGIELIASENFVCRAVM 180

Query: 181 EALGSHLTNKYSEGMPGARYYCGNQYIDEIETLCRERALAAFGLSSDNWGVNVQPYSCTS 240
           EALGSHLTNKYSEGMPGARYY GNQYID+IE LC ERAL AFGL SD WGVNVQPYSCTS
Sbjct: 181 EALGSHLTNKYSEGMPGARYYTGNQYIDQIENLCIERALTAFGLESDKWGVNVQPYSCTS 240

Query: 241 ANFAVYTGLLLPGDRIMGLDTPSGGNTSHGYCTPNGRKVSGASIFFESLPYKECVSP-FG 290
           ANFAVYTGLLLPG+RIMGLD+PSGG+ SHGYCTP G+K+S ASIFFES PYK  V+P  G
Sbjct: 241 ANFAVYTGLLLPGERIMGLDSPSGGHMSHGYCTPGGKKISAASIFFESFPYK--VNPQTG 295

BLAST of Cp4.1LG01g01850 vs. Swiss-Prot
Match: GLYC6_ARATH (Serine hydroxymethyltransferase 6 OS=Arabidopsis thaliana GN=SHM6 PE=2 SV=1)

HSP 1 Score: 354.8 bits (909), Expect = 1.6e-96
Identity = 193/299 (64.55%), Postives = 225/299 (75.25%), Query Frame = 1

Query: 6   SHSNLSLGF-SSHASP---PHRAQIADDSVLLHLDSSRRESSYSSTPVPLQLMEQQTES- 65
           + S+LSLGF SSHA P   P R  IADDS+ L +DSS R SS    PVPLQL+EQ+ +  
Sbjct: 5   AQSDLSLGFGSSHALPLPHPPRIPIADDSITLQIDSSFRPSSNPMPPVPLQLLEQRFDVT 64

Query: 66  -------HRNDGGDGERDDDEYDDREVEEFRILGHPMCLKRRRDGESST--SSGSAKRIS 125
                    +D   G+ DDD  D RE E+F +LGHPM LKR R G S +  SS   KR  
Sbjct: 65  GSCSRVVEEDDEVVGDNDDD--DQREEEQFILLGHPMKLKRGRGGNSYSLASSSPCKRFV 124

Query: 126 CEFDIETRRSAVRSWGDQTLRVADPNLFGIMEKEKQRQFKGIELIASENYVCRAVMEALG 185
            +  IE+RR+ VR+WG+Q++  ADP +   MEKEKQRQF+GIELIASEN+VCRAVMEALG
Sbjct: 125 VDSGIESRRAVVRAWGNQSIEEADPEIHEFMEKEKQRQFRGIELIASENFVCRAVMEALG 184

Query: 186 SHLTNKYSEGMPGARYYCGNQYIDEIETLCRERALAAFGLSSDNWGVNVQPYSCTSANFA 245
           SHLTNKYSEGMPGARYY GNQYID+IE LC+ERALAAFGL+ + WGVNVQPYSCTSANFA
Sbjct: 185 SHLTNKYSEGMPGARYYTGNQYIDQIEILCQERALAAFGLNHEKWGVNVQPYSCTSANFA 244

Query: 246 VYTGLLLPGDRIMGLDTPSGGNTSHGYCTPNGRKVSGASIFFESLPYKECVSP-FGYCD 290
           V+TGLL+PG+RIMGLD+PSGG+ SHGY TP G+KVSGASIFFES PYK  V P  GY D
Sbjct: 245 VFTGLLMPGERIMGLDSPSGGHMSHGYYTPGGKKVSGASIFFESFPYK--VDPRTGYID 299

BLAST of Cp4.1LG01g01850 vs. Swiss-Prot
Match: GLYC5_ARATH (Serine hydroxymethyltransferase 5 OS=Arabidopsis thaliana GN=SHM5 PE=3 SV=1)

HSP 1 Score: 251.9 bits (642), Expect = 1.4e-65
Identity = 127/244 (52.05%), Postives = 157/244 (64.34%), Query Frame = 1

Query: 279 KECVSPFGYCDVVTSTTHKSLRGPRGGIIFYRKGSKPRKRAMLLYQGNETDQYDFEEKIN 338
           +E   PF YCDVVT++THKSLRGPR G+IFYRKG KP K+        E + YDF+ KIN
Sbjct: 226 QEAADPFEYCDVVTTSTHKSLRGPRAGMIFYRKGPKPAKKGQ-----PEGEVYDFDAKIN 285

Query: 339 FAVFPSLQGGPHNNHIAALAVALKQVATPEYKAYMQQVKKNAQALAAALLRKKYKLVTGG 398
            AVFP+LQ GPHNN I ALAVALKQV  P +K Y +QVK NA  LA+ L+ K Y LVT G
Sbjct: 286 SAVFPALQSGPHNNKIGALAVALKQVMAPSFKVYAKQVKANAACLASYLINKGYTLVTDG 345

Query: 399 TDNHMILWDLRSLGLTGKNYENVCEICHITLNKITIFGDNGIIIPGGVRV---------- 458
           TDNH+ILWDLR LGLTG   E VCE+C+ITLN+  +FGD   + PGGVR+          
Sbjct: 346 TDNHLILWDLRPLGLTGNKVEKVCELCYITLNRNAVFGDTSFLAPGGVRIGTPAMTSRGL 405

Query: 459 --------------AAQIAISVQREYGKLPKTYLKGLLSNNEVVELRNRVESFSAQFAMP 499
                         A  I + +Q +YGK+ K + KGL++N E+ E++  VE F+  F MP
Sbjct: 406 VEKDFEKIGEFLHRAVTITLDIQEQYGKVMKDFNKGLVNNKEIDEIKADVEEFTYDFDMP 464

BLAST of Cp4.1LG01g01850 vs. Swiss-Prot
Match: GLYC4_ARATH (Serine hydroxymethyltransferase 4 OS=Arabidopsis thaliana GN=SHM4 PE=1 SV=1)

HSP 1 Score: 251.1 bits (640), Expect = 2.5e-65
Identity = 125/242 (51.65%), Postives = 160/242 (66.12%), Query Frame = 1

Query: 279 KECVSPFGYCDVVTSTTHKSLRGPRGGIIFYRKGSKPRKRAMLLYQGNETDQYDFEEKIN 338
           +E  +PF YCDVVT+TTHKSLRGPR G+IFYRKG KP K+        E   YDFE+KIN
Sbjct: 226 QEAANPFEYCDVVTTTTHKSLRGPRAGMIFYRKGPKPPKKGQ-----PEGAVYDFEDKIN 285

Query: 339 FAVFPSLQGGPHNNHIAALAVALKQVATPEYKAYMQQVKKNAQALAAALLRKKYKLVTGG 398
           FAVFP+LQGGPHN+ I ALAVALKQ  TP +K Y +QVK NA AL   L+ K Y++VT G
Sbjct: 286 FAVFPALQGGPHNHQIGALAVALKQANTPGFKVYAKQVKANAVALGNYLMSKGYQIVTNG 345

Query: 399 TDNHMILWDLRSLGLTGKNYENVCEICHITLNKITIFGDNGIIIPGGVRV---------- 458
           T+NH++LWDLR LGLTG   E +C++C ITLNK  +FGD+  + PGGVR+          
Sbjct: 346 TENHLVLWDLRPLGLTGNKVEKLCDLCSITLNKNAVFGDSSALAPGGVRIGAPAMTSRGL 405

Query: 459 --------------AAQIAISVQREYGKLPKTYLKGLLSNNEVVELRNRVESFSAQFAMP 497
                         A  + + +Q+ YGKL K + KGL++N ++ +L+  VE FSA + MP
Sbjct: 406 VEKDFEQIGEFLSRAVTLTLDIQKTYGKLLKDFNKGLVNNKDLDQLKADVEKFSASYEMP 462

BLAST of Cp4.1LG01g01850 vs. Swiss-Prot
Match: GLYA2_SULDN (Serine hydroxymethyltransferase 2 OS=Sulfurimonas denitrificans (strain ATCC 33889 / DSM 1251) GN=glyA2 PE=3 SV=1)

HSP 1 Score: 214.2 bits (544), Expect = 3.3e-54
Identity = 136/382 (35.60%), Postives = 191/382 (50.00%), Query Frame = 1

Query: 129 QTLRVADPNLFGIMEKEKQRQFKGIELIASENYVCRAVMEALGSHLTNKYSEGMPGARYY 188
           ++L  AD  +F I+E E +RQ   +E+IASEN+   AVMEA+GS  TNKY+EG P  RYY
Sbjct: 7   KSLEHADREVFSIIEGELKRQTAHLEMIASENFTSPAVMEAMGSVFTNKYAEGYPYKRYY 66

Query: 189 CGNQYIDEIETLCRERALAAFGLSSDNWGVNVQPYSCTSANFAVYTGLLLPGDRIMGLDT 248
            G +Y D +E L  +RA   FG        NVQP+S + AN AVY  LL  GD+++G+D 
Sbjct: 67  GGCEYADAVEQLAIDRACEIFGCKF----ANVQPHSGSQANAAVYAALLKAGDKLLGMDL 126

Query: 249 PSGGNTSHGY---------------CTPNGRK-----------------VSGASIFFESL 308
             GG+ +HG                   +GR                  V GAS +   +
Sbjct: 127 SHGGHLTHGSKPSFSGQNYSSFTYGVEADGRMDYDKILEIAKAVQPKIIVCGASAYAREI 186

Query: 309 PYK---------------------------ECVSPFGYCDVVTSTTHKSLRGPRGGIIFY 368
            +K                           E +SPF +  VVT+TTHK+LRGPRGG+I  
Sbjct: 187 DFKKFREIADAVGAFLFADIAHVAGLVAADEHMSPFPHAHVVTTTTHKTLRGPRGGMIM- 246

Query: 369 RKGSKPRKRAMLLYQGNETDQYDFEEKINFAVFPSLQGGPHNNHIAALAVALKQVATPEY 428
                             TD  +  +KIN A+FP +QGGP  + IAA AVA K++  P +
Sbjct: 247 ------------------TDDEEIAKKINSAIFPGIQGGPLVHVIAAKAVAFKEILDPSW 306

Query: 429 KAYMQQVKKNAQALAAALLRKKYKLVTGGTDNHMILWDLRSLGLTGKNYENVCEICHITL 451
           + Y +QVK NA+ L   L ++ Y LV+GGTDNH++L    +   +GK+ +   E   IT+
Sbjct: 307 REYAKQVKANAKVLEVVLTKRGYDLVSGGTDNHLVLVSFLNRDFSGKDADAALERAGITV 365

BLAST of Cp4.1LG01g01850 vs. TrEMBL
Match: A0A0A0LAD1_CUCSA (Serine hydroxymethyltransferase OS=Cucumis sativus GN=Csa_3G221750 PE=3 SV=1)

HSP 1 Score: 736.5 bits (1900), Expect = 2.2e-209
Identity = 385/587 (65.59%), Postives = 429/587 (73.08%), Query Frame = 1

Query: 1   MDLSKSHSNLSLGFSSHASPPHRAQIADDSVLLHLDSSRRESSYSSTPVPLQLMEQQTES 60
           MDLS S SN+S G     SPPHR QIADDS+LLHLDSSRR  +YS+ PVPLQLME QTE+
Sbjct: 1   MDLSNSQSNISRG---RVSPPHRTQIADDSILLHLDSSRRGPTYSAPPVPLQLMEPQTEN 60

Query: 61  HRNDGGDGERDDDEYDDREVEEFRILGHPMCLKRRRDGESSTSSGSAKRISCEFDIETRR 120
           HR++ GD +RDDDE DDR+VEEFRILGH MCLKRRRDGESS+  GS KR+SCEFD+ETRR
Sbjct: 61  HRDENGDAKRDDDECDDRDVEEFRILGHSMCLKRRRDGESSSLLGSTKRVSCEFDMETRR 120

Query: 121 SAVRSWGDQTLRVADPNLFGIMEKEKQRQFKGIELIASENYVCRAVMEALGSHLTNKYSE 180
           S+VRSWG+QTLR ADP L+GIMEKEKQRQ  GIELIASENYVCRAVMEALGSHLTNKYSE
Sbjct: 121 SSVRSWGNQTLRAADPKLYGIMEKEKQRQINGIELIASENYVCRAVMEALGSHLTNKYSE 180

Query: 181 GMPGARYYCGNQYIDEIETLCRERALAAFGLSSDNWGVNVQPYSCTSANFAVYTGLLLPG 240
           GMPGARYY GNQYIDEIE LCRERALAAF L+  +WGVNVQPYSCTSANFAVYTGLLLPG
Sbjct: 181 GMPGARYYGGNQYIDEIEILCRERALAAFDLNPHSWGVNVQPYSCTSANFAVYTGLLLPG 240

Query: 241 DRIMGLDTPSGGNTSHGYCTPNGRKVS--------------------------------- 300
           DRIM LD+PSGGN SHGYCT NG+KV+                                 
Sbjct: 241 DRIMALDSPSGGNPSHGYCTRNGKKVAGASIFFESFPYKVNPQTGYIDYDKLEERALDFR 300

Query: 301 ------GASIFFESLPY---------------------------KECVSPFGYCDVVTST 360
                 G S +   L Y                           KECVSPF YCDVVTST
Sbjct: 301 PKLLICGGSAYPRELDYARFRQIADKCGAVLMCDMAQISGLVAAKECVSPFEYCDVVTST 360

Query: 361 THKSLRGPRGGIIFYRKGSKPRKRAMLLYQGNETDQYDFEEKINFAVFPSLQGGPHNNHI 420
           THKSLRGPRGGIIFY+KG KPR RA++LYQGN+  +YDFEE+INFAVFPSLQGGPHNNHI
Sbjct: 361 THKSLRGPRGGIIFYKKGYKPRGRAIVLYQGNDVKEYDFEERINFAVFPSLQGGPHNNHI 420

Query: 421 AALAVALKQVATPEYKAYMQQVKKNAQALAAALLRKKYKLVTGGTDNHMILWDLRSLGLT 480
           AALAVALKQVAT EYK YMQQVKKNAQALA+ALLR+ YKLVTGGTDNHM+LWDLRSLGL+
Sbjct: 421 AALAVALKQVATLEYKGYMQQVKKNAQALASALLRRNYKLVTGGTDNHMVLWDLRSLGLS 480

Query: 481 GKNYENVCEICHITLNKITIFGDNGIIIPGGVRVAAQIAIS---VQREYGKLPK------ 498
           GKN E +CE+CHITLNKI I GDNG+I  GGVR+      S   ++ ++  + +      
Sbjct: 481 GKNLEQLCEMCHITLNKIIISGDNGVITTGGVRIGTPAMTSRGCLESDFELIVEFLHTAA 540

BLAST of Cp4.1LG01g01850 vs. TrEMBL
Match: I1KUN3_SOYBN (Serine hydroxymethyltransferase OS=Glycine max GN=GLYMA_08G187800 PE=3 SV=1)

HSP 1 Score: 734.2 bits (1894), Expect = 1.1e-208
Identity = 383/524 (73.09%), Postives = 422/524 (80.53%), Query Frame = 1

Query: 1   MDLSKSHSNLSLGFSS-HASPPHRAQIADDSVLLHLDSSRRESSYSSTPVPLQLMEQQTE 60
           MDLS   SNLSLGFSS HASPP R                     S  PVPLQLME QTE
Sbjct: 1   MDLSHPQSNLSLGFSSSHASPPRR---------------------SDPPVPLQLMEPQTE 60

Query: 61  SHRNDGGDGERDDDEYDDREVEEFRILGHPMCLKRRRDGESSTSSGSA-KRISCEFDIET 120
           +  N   D E DDD+ DD+EVEEFRILGH MCLKRRRD +SS+SS +A KR+S E D++ 
Sbjct: 61  NGNNL--DVESDDDD-DDKEVEEFRILGHSMCLKRRRDCDSSSSSAAAAKRVSVEPDLDA 120

Query: 121 RRSAVRSWGDQTLRVADPNLFGIMEKEKQRQFKGIELIASENYVCRAVMEALGSHLTNKY 180
           R++AVR+WG Q L +ADP++  IMEKEK+RQF GIELIASEN+VCRAVMEALGSHLTNKY
Sbjct: 121 RKAAVRAWGCQPLSIADPDIHEIMEKEKKRQFCGIELIASENFVCRAVMEALGSHLTNKY 180

Query: 181 SEGMPGARYYCGNQYIDEIETLCRERALAAFGLSSDNWGVNVQPYSCTSANFAVYTGLLL 240
           SEGMPG+RYY GNQYIDEIETLC ERAL AFGL    WGVNVQPYSCTSANF+VYTGLLL
Sbjct: 181 SEGMPGSRYYGGNQYIDEIETLCCERALNAFGLDPKCWGVNVQPYSCTSANFSVYTGLLL 240

Query: 241 PGDRIMGLDTPSGGNTSHGYCTPNGRKVSGASIFFESLPYKECVSPFGYCDVVTSTTHKS 300
           PGDRIMGLDTPSGGNTSHGY TPNG+KVSGASIFFESLPYKECV+PF YCD+VTSTTHKS
Sbjct: 241 PGDRIMGLDTPSGGNTSHGYYTPNGKKVSGASIFFESLPYKECVNPFDYCDIVTSTTHKS 300

Query: 301 LRGPRGGIIFYRKGSKPRKRAMLLYQGNETDQYDFEEKINFAVFPSLQGGPHNNHIAALA 360
           LRGPRGGIIFYRKG+KPR R +LL QG+E+DQYDFEEKINFAVFPS+QGGPHNNHIAALA
Sbjct: 301 LRGPRGGIIFYRKGTKPRNRGILLSQGHESDQYDFEEKINFAVFPSMQGGPHNNHIAALA 360

Query: 361 VALKQVATPEYKAYMQQVKKNAQALAAALLRKKYKLVTGGTDNHMILWDLRSLGLTGKNY 420
           +ALKQVATPEYKAYMQQVKKNAQALA ALLR+K +LVTGGTDNH+ILWDLR LGLTGK Y
Sbjct: 361 IALKQVATPEYKAYMQQVKKNAQALACALLRRKCRLVTGGTDNHLILWDLRPLGLTGKFY 420

Query: 421 ENVCEICHITLNKITIFGDNGIIIPGGVRV------------------------AAQIAI 480
           E VCE CHITLNKI IFGDNG IIPGGVRV                        AAQIA 
Sbjct: 421 EKVCETCHITLNKIAIFGDNGTIIPGGVRVGTPAMTSRGCLEADFETMAEFLIRAAQIAS 480

Query: 481 SVQREYGKLPKTYLKGLLSNNEVVELRNRVESFSAQFAMPGFEI 499
            +QRE+GKL KT LKGL S+ ++VELR RVE+F+ QFAMPGF+I
Sbjct: 481 ILQREHGKLQKTTLKGLESHRDIVELRARVEAFATQFAMPGFDI 500

BLAST of Cp4.1LG01g01850 vs. TrEMBL
Match: A0A061FNN6_THECC (Serine hydroxymethyltransferase OS=Theobroma cacao GN=TCM_034999 PE=3 SV=1)

HSP 1 Score: 664.8 bits (1714), Expect = 8.0e-188
Identity = 345/522 (66.09%), Postives = 399/522 (76.44%), Query Frame = 1

Query: 1   MDLSKSHSNLSLGFSSHASPPHRAQIADDSVLLHLDSSRRESSYSSTPVPLQLMEQQTES 60
           MDLS + S LSLGF+       +  IADDS+ L +D+S R SS     VPLQ +E     
Sbjct: 1   MDLSHAQSALSLGFT-------KTSIADDSISLQIDTSFRCSSNHVRRVPLQFLE----- 60

Query: 61  HRNDGGDGERDDDEYDD-REVEEFRILGHPMCLKRRRDGESSTSSGSAKRISCEFDIETR 120
            ++DG    +     +  +E EE RILG  MC+KR RD +S   S   KR++ E  ++ R
Sbjct: 61  GKDDGAPRFQSGKHINHIKEDEEIRILGQSMCVKRHRDSQS--VSNLCKRLATEPSLDQR 120

Query: 121 RSAVRSWGDQTLRVADPNLFGIMEKEKQRQFKGIELIASENYVCRAVMEALGSHLTNKYS 180
           R+AVRSWG+Q L  ADP L  IM KEKQRQF GIELIASEN+VCRAVMEALGSHLTNKYS
Sbjct: 121 RAAVRSWGNQRLEEADPELSEIMNKEKQRQFFGIELIASENFVCRAVMEALGSHLTNKYS 180

Query: 181 EGMPGARYYCGNQYIDEIETLCRERALAAFGLSSDNWGVNVQPYSCTSANFAVYTGLLLP 240
           EGMPGARYY GN +ID+IETLC ERALAAF L S+ WGVNVQPYSCTSANFAVYTGLLLP
Sbjct: 181 EGMPGARYYTGNHFIDQIETLCHERALAAFSLDSEKWGVNVQPYSCTSANFAVYTGLLLP 240

Query: 241 GDRIMGLDTPSGGNTSHGYCTPNGRKVSGASIFFESLPYKECVSPFGYCDVVTSTTHKSL 300
           GDRIMGLD+PSGG+ SHG+  P G+KVS ASIFFES PYKEC SPF YCD+VTSTTHKSL
Sbjct: 241 GDRIMGLDSPSGGHMSHGWYMPGGKKVSAASIFFESFPYKECASPFDYCDIVTSTTHKSL 300

Query: 301 RGPRGGIIFYRKGSKPRKRAMLLYQGNETDQYDFEEKINFAVFPSLQGGPHNNHIAALAV 360
           RGPRGGIIFYR+G+K RK+ M L  G+   QYDFEEKINFAVFPSLQGGPHNNHIAALA+
Sbjct: 301 RGPRGGIIFYRRGAKSRKQGMSLSNGDCGCQYDFEEKINFAVFPSLQGGPHNNHIAALAI 360

Query: 361 ALKQVATPEYKAYMQQVKKNAQALAAALLRKKYKLVTGGTDNHMILWDLRSLGLTGKNYE 420
           ALK VATPEYKAYMQQVKKNAQALA+AL+R+K KLVTGGTDNH++LWDL +LGLTGK YE
Sbjct: 361 ALKHVATPEYKAYMQQVKKNAQALASALVRRKCKLVTGGTDNHLLLWDLTTLGLTGKCYE 420

Query: 421 NVCEICHITLNKITIFGDNGIIIPGGVRV------------------------AAQIAIS 480
            VCE+CHITLNK  IFGDNG I PGGVR+                        AAQIA +
Sbjct: 421 KVCEMCHITLNKTAIFGDNGAICPGGVRIGSPAMTSRGCLESDFETMADFLYRAAQIASA 480

Query: 481 VQREYGKLPKTYLKGLLSNNEVVELRNRVESFSAQFAMPGFE 498
           VQR++GKL K +LKGL +N ++V+LRN+VE+F+AQFAMPGF+
Sbjct: 481 VQRDHGKLQKEFLKGLHNNKDIVDLRNQVEAFAAQFAMPGFD 508

BLAST of Cp4.1LG01g01850 vs. TrEMBL
Match: K3ZHZ4_SETIT (Serine hydroxymethyltransferase OS=Setaria italica GN=SETIT_026132mg PE=3 SV=1)

HSP 1 Score: 472.2 bits (1214), Expect = 7.6e-130
Identity = 233/400 (58.25%), Postives = 285/400 (71.25%), Query Frame = 1

Query: 123 VRSWGDQTLRVADPNLFGIMEKEKQRQFKGIELIASENYVCRAVMEALGSHLTNKYSEGM 182
           V +WG   L  ADP ++ ++E+EK+RQ +GIELIASEN+   AVMEALGS LTNKYSEGM
Sbjct: 135 VATWGLTPLAGADPEIYDLLEREKRRQRRGIELIASENFTSFAVMEALGSALTNKYSEGM 194

Query: 183 PGARYYCGNQYIDEIETLCRERALAAFGLSSDNWGVNVQPYSCTSANFAVYTGLLLPGDR 242
           PGARYY GN  IDEIE LCR RALAAF L   +WGVNVQPYS + ANFA YT LL P DR
Sbjct: 195 PGARYYGGNDVIDEIENLCRSRALAAFRLDPASWGVNVQPYSGSPANFAAYTALLNPHDR 254

Query: 243 IMGLDTPSGGNTSHGYCTPNGRKVSGASIFFESLPYKECVSPFGYCDVVTSTTHKSLRGP 302
           IMGLD PSGG+ +HGY T  G+K+S  SI+FESLPYKE  +PF YCDVVT+TTHKSLRGP
Sbjct: 255 IMGLDLPSGGHLTHGYYTSGGKKISATSIYFESLPYKEAANPFEYCDVVTTTTHKSLRGP 314

Query: 303 RGGIIFYRKGSKPRKRAMLLYQGNETDQYDFEEKINFAVFPSLQGGPHNNHIAALAVALK 362
           R G+IFYRKG KP K+        E   YD+E+KINFAVFPSLQGGPHN+ IAALAVAL+
Sbjct: 315 RAGMIFYRKGPKPPKKGQ-----PEGAVYDYEDKINFAVFPSLQGGPHNHQIAALAVALQ 374

Query: 363 QVATPEYKAYMQQVKKNAQALAAALLRKKYKLVTGGTDNHMILWDLRSLGLTGKNYENVC 422
           Q  TP +KAY +QVK NA A+   L+ K YK+VT GT+NH++LWDLR LGLTG   E +C
Sbjct: 375 QTMTPGFKAYAKQVKANAVAIGNYLMSKGYKMVTDGTENHLVLWDLRPLGLTGNKVEKLC 434

Query: 423 EICHITLNKITIFGDNGIIIPGGVRV------------------------AAQIAISVQR 482
           ++CHITLNK  +FGD+  + PGGVR+                        A  I +++Q+
Sbjct: 435 DLCHITLNKNAVFGDSSALSPGGVRIGAPAMTSRGLVEKDFEQIGEFLHRAVTICLNIQK 494

Query: 483 EYGKLPKTYLKGLLSNNEVVELRNRVESFSAQFAMPGFEI 499
           E+GKL K + KGL++N ++  L+  VE F+  F MPGF +
Sbjct: 495 EHGKLLKDFNKGLVNNKDIENLKAEVEKFADSFDMPGFTL 529

BLAST of Cp4.1LG01g01850 vs. TrEMBL
Match: A9P855_POPTR (Serine hydroxymethyltransferase OS=Populus trichocarpa PE=2 SV=1)

HSP 1 Score: 423.7 bits (1088), Expect = 3.1e-115
Identity = 226/466 (48.50%), Postives = 279/466 (59.87%), Query Frame = 1

Query: 123 VRSWGDQTLRVADPNLFGIMEKEKQRQFKGIELIASENYVCRAVMEALGSHLTNKYSEGM 182
           V +WG+ +L   DP +  ++EKEK+RQ +GIELIASEN+   AV+EALGS LTNKYSEGM
Sbjct: 4   VTAWGNTSLESVDPEIHDLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYSEGM 63

Query: 183 PGARYYCGNQYIDEIETLCRERALAAFGLSSDNWGVNVQPYSCTSANFAVYTGLLLPGDR 242
           PG RYY GN+YID+IE LCR RAL AF L    WGVNVQPYS + ANFA YT +L P DR
Sbjct: 64  PGNRYYGGNEYIDQIENLCRSRALEAFHLDPTKWGVNVQPYSGSPANFAAYTAVLQPHDR 123

Query: 243 IMGLDTPSGGNTSHGYCTPNGRKVS----------------------------------- 302
           IMGLD PSGG+ +HGY T  G+K+S                                   
Sbjct: 124 IMGLDLPSGGHLTHGYYTSGGKKISATSIYFESLPYKVNSQSGYIDYDKLEEKALDFRPK 183

Query: 303 ----GASIFFESLPYK---------------------------ECVSPFGYCDVVTSTTH 362
               G S +     YK                           E  +PF YCD+VT+TTH
Sbjct: 184 LIICGGSAYPRDWDYKKFRSVADKCGALLLCDMAHISGLVAAQEAANPFEYCDIVTTTTH 243

Query: 363 KSLRGPRGGIIFYRKGSKPRKRAMLLYQGNETDQYDFEEKINFAVFPSLQGGPHNNHIAA 422
           KSLRGPR G+IFYRKG KP K+        E   YDFE+KINFAVFPSLQGGPHN+ I A
Sbjct: 244 KSLRGPRAGMIFYRKGPKPPKKGQ-----PENAVYDFEDKINFAVFPSLQGGPHNHQIGA 303

Query: 423 LAVALKQVATPEYKAYMQQVKKNAQALAAALLRKKYKLVTGGTDNHMILWDLRSLGLTGK 482
           LAVALKQV TP +KAY +QVK NA AL   L+ + YKLVT GT+NH++LWDLR LGLTG 
Sbjct: 304 LAVALKQVQTPGFKAYAKQVKANAVALGNYLMGQGYKLVTEGTENHLVLWDLRPLGLTGN 363

Query: 483 NYENVCEICHITLNKITIFGDNGIIIPGGVRV------------------------AAQI 499
             E +C++ +IT+NK  +FGD+  + PGGVR+                        A  I
Sbjct: 364 KVEKLCDLANITVNKNAVFGDSSALAPGGVRIGTPAMTSRGLVEKDFEQIGEFLHRAVTI 423

BLAST of Cp4.1LG01g01850 vs. TAIR10
Match: AT1G36370.1 (AT1G36370.1 serine hydroxymethyltransferase 7)

HSP 1 Score: 362.5 bits (929), Expect = 4.3e-100
Identity = 196/303 (64.69%), Postives = 227/303 (74.92%), Query Frame = 1

Query: 1   MDLSKSHSNLSLGFS-SHAS----PPHRAQIADDSVLLHLDSSRRESSYSSTPVPLQLME 60
           MDLS+S +N  LGF  SHAS    P  RA IADDS+ L +D S R    + +P+PLQL+E
Sbjct: 1   MDLSRSQTNFQLGFGCSHASMTPTPTPRAPIADDSINLQVDQSFRSLPTTFSPIPLQLLE 60

Query: 61  QQTESH------RNDGGDGERDDDEYDDREVEEFRILGHPMCLKRRRDGESS-TSSGSAK 120
           Q+ E        + DGG G       D +E E FRILGH MCLKR+RD     T S   K
Sbjct: 61  QKAEKTTTVDEPKKDGGGGG------DQKEDEHFRILGHHMCLKRQRDCPLLLTQSKHPK 120

Query: 121 RISC-EFDIETRRSAVRSWGDQTLRVADPNLFGIMEKEKQRQFKGIELIASENYVCRAVM 180
           R S  + D+E+RR+AVR+WGDQ + +ADP++  +MEKEKQRQ +GIELIASEN+VCRAVM
Sbjct: 121 RSSIGDSDLESRRAAVRAWGDQPIHLADPDIHELMEKEKQRQVRGIELIASENFVCRAVM 180

Query: 181 EALGSHLTNKYSEGMPGARYYCGNQYIDEIETLCRERALAAFGLSSDNWGVNVQPYSCTS 240
           EALGSHLTNKYSEGMPGARYY GNQYID+IE LC ERAL AFGL SD WGVNVQPYSCTS
Sbjct: 181 EALGSHLTNKYSEGMPGARYYTGNQYIDQIENLCIERALTAFGLESDKWGVNVQPYSCTS 240

Query: 241 ANFAVYTGLLLPGDRIMGLDTPSGGNTSHGYCTPNGRKVSGASIFFESLPYKECVSP-FG 290
           ANFAVYTGLLLPG+RIMGLD+PSGG+ SHGYCTP G+K+S ASIFFES PYK  V+P  G
Sbjct: 241 ANFAVYTGLLLPGERIMGLDSPSGGHMSHGYCTPGGKKISAASIFFESFPYK--VNPQTG 295

BLAST of Cp4.1LG01g01850 vs. TAIR10
Match: AT1G22020.1 (AT1G22020.1 serine hydroxymethyltransferase 6)

HSP 1 Score: 354.8 bits (909), Expect = 8.9e-98
Identity = 193/299 (64.55%), Postives = 225/299 (75.25%), Query Frame = 1

Query: 6   SHSNLSLGF-SSHASP---PHRAQIADDSVLLHLDSSRRESSYSSTPVPLQLMEQQTES- 65
           + S+LSLGF SSHA P   P R  IADDS+ L +DSS R SS    PVPLQL+EQ+ +  
Sbjct: 5   AQSDLSLGFGSSHALPLPHPPRIPIADDSITLQIDSSFRPSSNPMPPVPLQLLEQRFDVT 64

Query: 66  -------HRNDGGDGERDDDEYDDREVEEFRILGHPMCLKRRRDGESST--SSGSAKRIS 125
                    +D   G+ DDD  D RE E+F +LGHPM LKR R G S +  SS   KR  
Sbjct: 65  GSCSRVVEEDDEVVGDNDDD--DQREEEQFILLGHPMKLKRGRGGNSYSLASSSPCKRFV 124

Query: 126 CEFDIETRRSAVRSWGDQTLRVADPNLFGIMEKEKQRQFKGIELIASENYVCRAVMEALG 185
            +  IE+RR+ VR+WG+Q++  ADP +   MEKEKQRQF+GIELIASEN+VCRAVMEALG
Sbjct: 125 VDSGIESRRAVVRAWGNQSIEEADPEIHEFMEKEKQRQFRGIELIASENFVCRAVMEALG 184

Query: 186 SHLTNKYSEGMPGARYYCGNQYIDEIETLCRERALAAFGLSSDNWGVNVQPYSCTSANFA 245
           SHLTNKYSEGMPGARYY GNQYID+IE LC+ERALAAFGL+ + WGVNVQPYSCTSANFA
Sbjct: 185 SHLTNKYSEGMPGARYYTGNQYIDQIEILCQERALAAFGLNHEKWGVNVQPYSCTSANFA 244

Query: 246 VYTGLLLPGDRIMGLDTPSGGNTSHGYCTPNGRKVSGASIFFESLPYKECVSP-FGYCD 290
           V+TGLL+PG+RIMGLD+PSGG+ SHGY TP G+KVSGASIFFES PYK  V P  GY D
Sbjct: 245 VFTGLLMPGERIMGLDSPSGGHMSHGYYTPGGKKVSGASIFFESFPYK--VDPRTGYID 299

BLAST of Cp4.1LG01g01850 vs. TAIR10
Match: AT4G13890.1 (AT4G13890.1 Pyridoxal phosphate (PLP)-dependent transferases superfamily protein)

HSP 1 Score: 251.9 bits (642), Expect = 8.2e-67
Identity = 127/244 (52.05%), Postives = 157/244 (64.34%), Query Frame = 1

Query: 279 KECVSPFGYCDVVTSTTHKSLRGPRGGIIFYRKGSKPRKRAMLLYQGNETDQYDFEEKIN 338
           +E   PF YCDVVT++THKSLRGPR G+IFYRKG KP K+        E + YDF+ KIN
Sbjct: 226 QEAADPFEYCDVVTTSTHKSLRGPRAGMIFYRKGPKPAKKGQ-----PEGEVYDFDAKIN 285

Query: 339 FAVFPSLQGGPHNNHIAALAVALKQVATPEYKAYMQQVKKNAQALAAALLRKKYKLVTGG 398
            AVFP+LQ GPHNN I ALAVALKQV  P +K Y +QVK NA  LA+ L+ K Y LVT G
Sbjct: 286 SAVFPALQSGPHNNKIGALAVALKQVMAPSFKVYAKQVKANAACLASYLINKGYTLVTDG 345

Query: 399 TDNHMILWDLRSLGLTGKNYENVCEICHITLNKITIFGDNGIIIPGGVRV---------- 458
           TDNH+ILWDLR LGLTG   E VCE+C+ITLN+  +FGD   + PGGVR+          
Sbjct: 346 TDNHLILWDLRPLGLTGNKVEKVCELCYITLNRNAVFGDTSFLAPGGVRIGTPAMTSRGL 405

Query: 459 --------------AAQIAISVQREYGKLPKTYLKGLLSNNEVVELRNRVESFSAQFAMP 499
                         A  I + +Q +YGK+ K + KGL++N E+ E++  VE F+  F MP
Sbjct: 406 VEKDFEKIGEFLHRAVTITLDIQEQYGKVMKDFNKGLVNNKEIDEIKADVEEFTYDFDMP 464

BLAST of Cp4.1LG01g01850 vs. TAIR10
Match: AT4G13930.1 (AT4G13930.1 serine hydroxymethyltransferase 4)

HSP 1 Score: 251.1 bits (640), Expect = 1.4e-66
Identity = 125/242 (51.65%), Postives = 160/242 (66.12%), Query Frame = 1

Query: 279 KECVSPFGYCDVVTSTTHKSLRGPRGGIIFYRKGSKPRKRAMLLYQGNETDQYDFEEKIN 338
           +E  +PF YCDVVT+TTHKSLRGPR G+IFYRKG KP K+        E   YDFE+KIN
Sbjct: 226 QEAANPFEYCDVVTTTTHKSLRGPRAGMIFYRKGPKPPKKGQ-----PEGAVYDFEDKIN 285

Query: 339 FAVFPSLQGGPHNNHIAALAVALKQVATPEYKAYMQQVKKNAQALAAALLRKKYKLVTGG 398
           FAVFP+LQGGPHN+ I ALAVALKQ  TP +K Y +QVK NA AL   L+ K Y++VT G
Sbjct: 286 FAVFPALQGGPHNHQIGALAVALKQANTPGFKVYAKQVKANAVALGNYLMSKGYQIVTNG 345

Query: 399 TDNHMILWDLRSLGLTGKNYENVCEICHITLNKITIFGDNGIIIPGGVRV---------- 458
           T+NH++LWDLR LGLTG   E +C++C ITLNK  +FGD+  + PGGVR+          
Sbjct: 346 TENHLVLWDLRPLGLTGNKVEKLCDLCSITLNKNAVFGDSSALAPGGVRIGAPAMTSRGL 405

Query: 459 --------------AAQIAISVQREYGKLPKTYLKGLLSNNEVVELRNRVESFSAQFAMP 497
                         A  + + +Q+ YGKL K + KGL++N ++ +L+  VE FSA + MP
Sbjct: 406 VEKDFEQIGEFLSRAVTLTLDIQKTYGKLLKDFNKGLVNNKDLDQLKADVEKFSASYEMP 462

BLAST of Cp4.1LG01g01850 vs. TAIR10
Match: AT4G32520.1 (AT4G32520.1 serine hydroxymethyltransferase 3)

HSP 1 Score: 203.0 bits (515), Expect = 4.3e-52
Identity = 106/188 (56.38%), Postives = 130/188 (69.15%), Query Frame = 1

Query: 92  LKRRRDGESSTSSGSAKRISCEFDIETRRSAVRSWGDQTLRVADPNLFGIMEKEKQRQFK 151
           LKR    E   SS S   +    +I ++      +G   L   DP +  I+ KEK RQF+
Sbjct: 51  LKRNLVSEMRASSVSLPNV----EISSKEIPFEDYG---LGEVDPEVRTIITKEKDRQFR 110

Query: 152 GIELIASENYVCRAVMEALGSHLTNKYSEGMPGARYYCGNQYIDEIETLCRERALAAFGL 211
            +ELIASEN+  RAVMEA+GS LTNKYSEG+PG RYY GN+YID++ETLC+ RALAAF L
Sbjct: 111 SLELIASENFTSRAVMEAVGSCLTNKYSEGLPGKRYYGGNEYIDQLETLCQNRALAAFRL 170

Query: 212 SSDNWGVNVQPYSCTSANFAVYTGLLLPGDRIMGLDTPSGGNTSHGYCTPNGRKVSGASI 271
            S  WGVNVQP S + ANFAVYT +L P DRIMGLD P GG+ SHG+ T   R+VSG SI
Sbjct: 171 DSTKWGVNVQPLSGSPANFAVYTAILSPHDRIMGLDLPHGGHLSHGFMTAK-RRVSGTSI 230

Query: 272 FFESLPYK 280
           +FES+PY+
Sbjct: 231 YFESMPYR 230

BLAST of Cp4.1LG01g01850 vs. NCBI nr
Match: gi|659112103|ref|XP_008456065.1| (PREDICTED: serine hydroxymethyltransferase 7-like [Cucumis melo])

HSP 1 Score: 740.7 bits (1911), Expect = 1.6e-210
Identity = 386/587 (65.76%), Postives = 430/587 (73.25%), Query Frame = 1

Query: 1   MDLSKSHSNLSLGFSSHASPPHRAQIADDSVLLHLDSSRRESSYSSTPVPLQLMEQQTES 60
           MDLS S SN+S G     SPPHR QIADDS+LLHLDSSRR  SYSS PVPLQLME QTE+
Sbjct: 1   MDLSNSQSNISRG---RVSPPHRTQIADDSILLHLDSSRRGPSYSSPPVPLQLMEPQTEN 60

Query: 61  HRNDGGDGERDDDEYDDREVEEFRILGHPMCLKRRRDGESSTSSGSAKRISCEFDIETRR 120
           HR++ GD +RD DE DDR+VEEFRILGH MCLKRRRDG+SS+  GS KR+SCEFD+ETRR
Sbjct: 61  HRDENGDAKRDGDECDDRDVEEFRILGHSMCLKRRRDGDSSSLLGSTKRVSCEFDMETRR 120

Query: 121 SAVRSWGDQTLRVADPNLFGIMEKEKQRQFKGIELIASENYVCRAVMEALGSHLTNKYSE 180
           S+VRSWG+QTLR ADP L+GIMEKEKQRQ  GIELIASENYVCRAVMEALGSHLTNKYSE
Sbjct: 121 SSVRSWGNQTLRAADPKLYGIMEKEKQRQINGIELIASENYVCRAVMEALGSHLTNKYSE 180

Query: 181 GMPGARYYCGNQYIDEIETLCRERALAAFGLSSDNWGVNVQPYSCTSANFAVYTGLLLPG 240
           GMPGARYY GNQYIDEIE LCRERALAAF L+  +WGVNVQPYSCTSANFAVYTGLLLPG
Sbjct: 181 GMPGARYYGGNQYIDEIEILCRERALAAFDLNPHSWGVNVQPYSCTSANFAVYTGLLLPG 240

Query: 241 DRIMGLDTPSGGNTSHGYCTPNGRKVS--------------------------------- 300
           DRIM LD+PSGGN SHGYCT NG+KV+                                 
Sbjct: 241 DRIMALDSPSGGNPSHGYCTRNGKKVAGASIFFESFPYKVNPQTGYIDYDKLEERALDFR 300

Query: 301 ------GASIFFESLPY---------------------------KECVSPFGYCDVVTST 360
                 G S +   L Y                           KEC SPF YCDVVTST
Sbjct: 301 PKLLICGGSAYPRELDYARFRQIADKCGAVLMCDMAQISGLVAAKECASPFEYCDVVTST 360

Query: 361 THKSLRGPRGGIIFYRKGSKPRKRAMLLYQGNETDQYDFEEKINFAVFPSLQGGPHNNHI 420
           THKSLRGPRGGIIFY+KG KPR RA++LYQGN+  +YDFEE+INFAVFPSLQGGPHNNHI
Sbjct: 361 THKSLRGPRGGIIFYKKGYKPRGRAIVLYQGNDVKEYDFEERINFAVFPSLQGGPHNNHI 420

Query: 421 AALAVALKQVATPEYKAYMQQVKKNAQALAAALLRKKYKLVTGGTDNHMILWDLRSLGLT 480
           AALAVAL+QVATPEYKAYMQQVKKNAQALA+ALLR+ YKLVTGGTDNHM+LWDLRSLGL+
Sbjct: 421 AALAVALRQVATPEYKAYMQQVKKNAQALASALLRRNYKLVTGGTDNHMVLWDLRSLGLS 480

Query: 481 GKNYENVCEICHITLNKITIFGDNGIIIPGGVRVAAQIAIS---VQREYGKLPK------ 498
           GKN E +CE+CHITLNKI IFGDNG+I  GGVR+      S   ++ ++  + +      
Sbjct: 481 GKNLEQLCEMCHITLNKIIIFGDNGVITTGGVRIGTPAMTSRGCLESDFELIVEFLHTAA 540

BLAST of Cp4.1LG01g01850 vs. NCBI nr
Match: gi|449457095|ref|XP_004146284.1| (PREDICTED: serine hydroxymethyltransferase 7-like [Cucumis sativus])

HSP 1 Score: 736.5 bits (1900), Expect = 3.1e-209
Identity = 385/587 (65.59%), Postives = 429/587 (73.08%), Query Frame = 1

Query: 1   MDLSKSHSNLSLGFSSHASPPHRAQIADDSVLLHLDSSRRESSYSSTPVPLQLMEQQTES 60
           MDLS S SN+S G     SPPHR QIADDS+LLHLDSSRR  +YS+ PVPLQLME QTE+
Sbjct: 1   MDLSNSQSNISRG---RVSPPHRTQIADDSILLHLDSSRRGPTYSAPPVPLQLMEPQTEN 60

Query: 61  HRNDGGDGERDDDEYDDREVEEFRILGHPMCLKRRRDGESSTSSGSAKRISCEFDIETRR 120
           HR++ GD +RDDDE DDR+VEEFRILGH MCLKRRRDGESS+  GS KR+SCEFD+ETRR
Sbjct: 61  HRDENGDAKRDDDECDDRDVEEFRILGHSMCLKRRRDGESSSLLGSTKRVSCEFDMETRR 120

Query: 121 SAVRSWGDQTLRVADPNLFGIMEKEKQRQFKGIELIASENYVCRAVMEALGSHLTNKYSE 180
           S+VRSWG+QTLR ADP L+GIMEKEKQRQ  GIELIASENYVCRAVMEALGSHLTNKYSE
Sbjct: 121 SSVRSWGNQTLRAADPKLYGIMEKEKQRQINGIELIASENYVCRAVMEALGSHLTNKYSE 180

Query: 181 GMPGARYYCGNQYIDEIETLCRERALAAFGLSSDNWGVNVQPYSCTSANFAVYTGLLLPG 240
           GMPGARYY GNQYIDEIE LCRERALAAF L+  +WGVNVQPYSCTSANFAVYTGLLLPG
Sbjct: 181 GMPGARYYGGNQYIDEIEILCRERALAAFDLNPHSWGVNVQPYSCTSANFAVYTGLLLPG 240

Query: 241 DRIMGLDTPSGGNTSHGYCTPNGRKVS--------------------------------- 300
           DRIM LD+PSGGN SHGYCT NG+KV+                                 
Sbjct: 241 DRIMALDSPSGGNPSHGYCTRNGKKVAGASIFFESFPYKVNPQTGYIDYDKLEERALDFR 300

Query: 301 ------GASIFFESLPY---------------------------KECVSPFGYCDVVTST 360
                 G S +   L Y                           KECVSPF YCDVVTST
Sbjct: 301 PKLLICGGSAYPRELDYARFRQIADKCGAVLMCDMAQISGLVAAKECVSPFEYCDVVTST 360

Query: 361 THKSLRGPRGGIIFYRKGSKPRKRAMLLYQGNETDQYDFEEKINFAVFPSLQGGPHNNHI 420
           THKSLRGPRGGIIFY+KG KPR RA++LYQGN+  +YDFEE+INFAVFPSLQGGPHNNHI
Sbjct: 361 THKSLRGPRGGIIFYKKGYKPRGRAIVLYQGNDVKEYDFEERINFAVFPSLQGGPHNNHI 420

Query: 421 AALAVALKQVATPEYKAYMQQVKKNAQALAAALLRKKYKLVTGGTDNHMILWDLRSLGLT 480
           AALAVALKQVAT EYK YMQQVKKNAQALA+ALLR+ YKLVTGGTDNHM+LWDLRSLGL+
Sbjct: 421 AALAVALKQVATLEYKGYMQQVKKNAQALASALLRRNYKLVTGGTDNHMVLWDLRSLGLS 480

Query: 481 GKNYENVCEICHITLNKITIFGDNGIIIPGGVRVAAQIAIS---VQREYGKLPK------ 498
           GKN E +CE+CHITLNKI I GDNG+I  GGVR+      S   ++ ++  + +      
Sbjct: 481 GKNLEQLCEMCHITLNKIIISGDNGVITTGGVRIGTPAMTSRGCLESDFELIVEFLHTAA 540

BLAST of Cp4.1LG01g01850 vs. NCBI nr
Match: gi|947095479|gb|KRH44064.1| (hypothetical protein GLYMA_08G187800 [Glycine max])

HSP 1 Score: 734.2 bits (1894), Expect = 1.5e-208
Identity = 383/524 (73.09%), Postives = 422/524 (80.53%), Query Frame = 1

Query: 1   MDLSKSHSNLSLGFSS-HASPPHRAQIADDSVLLHLDSSRRESSYSSTPVPLQLMEQQTE 60
           MDLS   SNLSLGFSS HASPP R                     S  PVPLQLME QTE
Sbjct: 1   MDLSHPQSNLSLGFSSSHASPPRR---------------------SDPPVPLQLMEPQTE 60

Query: 61  SHRNDGGDGERDDDEYDDREVEEFRILGHPMCLKRRRDGESSTSSGSA-KRISCEFDIET 120
           +  N   D E DDD+ DD+EVEEFRILGH MCLKRRRD +SS+SS +A KR+S E D++ 
Sbjct: 61  NGNNL--DVESDDDD-DDKEVEEFRILGHSMCLKRRRDCDSSSSSAAAAKRVSVEPDLDA 120

Query: 121 RRSAVRSWGDQTLRVADPNLFGIMEKEKQRQFKGIELIASENYVCRAVMEALGSHLTNKY 180
           R++AVR+WG Q L +ADP++  IMEKEK+RQF GIELIASEN+VCRAVMEALGSHLTNKY
Sbjct: 121 RKAAVRAWGCQPLSIADPDIHEIMEKEKKRQFCGIELIASENFVCRAVMEALGSHLTNKY 180

Query: 181 SEGMPGARYYCGNQYIDEIETLCRERALAAFGLSSDNWGVNVQPYSCTSANFAVYTGLLL 240
           SEGMPG+RYY GNQYIDEIETLC ERAL AFGL    WGVNVQPYSCTSANF+VYTGLLL
Sbjct: 181 SEGMPGSRYYGGNQYIDEIETLCCERALNAFGLDPKCWGVNVQPYSCTSANFSVYTGLLL 240

Query: 241 PGDRIMGLDTPSGGNTSHGYCTPNGRKVSGASIFFESLPYKECVSPFGYCDVVTSTTHKS 300
           PGDRIMGLDTPSGGNTSHGY TPNG+KVSGASIFFESLPYKECV+PF YCD+VTSTTHKS
Sbjct: 241 PGDRIMGLDTPSGGNTSHGYYTPNGKKVSGASIFFESLPYKECVNPFDYCDIVTSTTHKS 300

Query: 301 LRGPRGGIIFYRKGSKPRKRAMLLYQGNETDQYDFEEKINFAVFPSLQGGPHNNHIAALA 360
           LRGPRGGIIFYRKG+KPR R +LL QG+E+DQYDFEEKINFAVFPS+QGGPHNNHIAALA
Sbjct: 301 LRGPRGGIIFYRKGTKPRNRGILLSQGHESDQYDFEEKINFAVFPSMQGGPHNNHIAALA 360

Query: 361 VALKQVATPEYKAYMQQVKKNAQALAAALLRKKYKLVTGGTDNHMILWDLRSLGLTGKNY 420
           +ALKQVATPEYKAYMQQVKKNAQALA ALLR+K +LVTGGTDNH+ILWDLR LGLTGK Y
Sbjct: 361 IALKQVATPEYKAYMQQVKKNAQALACALLRRKCRLVTGGTDNHLILWDLRPLGLTGKFY 420

Query: 421 ENVCEICHITLNKITIFGDNGIIIPGGVRV------------------------AAQIAI 480
           E VCE CHITLNKI IFGDNG IIPGGVRV                        AAQIA 
Sbjct: 421 EKVCETCHITLNKIAIFGDNGTIIPGGVRVGTPAMTSRGCLEADFETMAEFLIRAAQIAS 480

Query: 481 SVQREYGKLPKTYLKGLLSNNEVVELRNRVESFSAQFAMPGFEI 499
            +QRE+GKL KT LKGL S+ ++VELR RVE+F+ QFAMPGF+I
Sbjct: 481 ILQREHGKLQKTTLKGLESHRDIVELRARVEAFATQFAMPGFDI 500

BLAST of Cp4.1LG01g01850 vs. NCBI nr
Match: gi|590598484|ref|XP_007018909.1| (Serine hydroxymethyltransferase 6 isoform 2 [Theobroma cacao])

HSP 1 Score: 664.8 bits (1714), Expect = 1.1e-187
Identity = 345/522 (66.09%), Postives = 399/522 (76.44%), Query Frame = 1

Query: 1   MDLSKSHSNLSLGFSSHASPPHRAQIADDSVLLHLDSSRRESSYSSTPVPLQLMEQQTES 60
           MDLS + S LSLGF+       +  IADDS+ L +D+S R SS     VPLQ +E     
Sbjct: 1   MDLSHAQSALSLGFT-------KTSIADDSISLQIDTSFRCSSNHVRRVPLQFLE----- 60

Query: 61  HRNDGGDGERDDDEYDD-REVEEFRILGHPMCLKRRRDGESSTSSGSAKRISCEFDIETR 120
            ++DG    +     +  +E EE RILG  MC+KR RD +S   S   KR++ E  ++ R
Sbjct: 61  GKDDGAPRFQSGKHINHIKEDEEIRILGQSMCVKRHRDSQS--VSNLCKRLATEPSLDQR 120

Query: 121 RSAVRSWGDQTLRVADPNLFGIMEKEKQRQFKGIELIASENYVCRAVMEALGSHLTNKYS 180
           R+AVRSWG+Q L  ADP L  IM KEKQRQF GIELIASEN+VCRAVMEALGSHLTNKYS
Sbjct: 121 RAAVRSWGNQRLEEADPELSEIMNKEKQRQFFGIELIASENFVCRAVMEALGSHLTNKYS 180

Query: 181 EGMPGARYYCGNQYIDEIETLCRERALAAFGLSSDNWGVNVQPYSCTSANFAVYTGLLLP 240
           EGMPGARYY GN +ID+IETLC ERALAAF L S+ WGVNVQPYSCTSANFAVYTGLLLP
Sbjct: 181 EGMPGARYYTGNHFIDQIETLCHERALAAFSLDSEKWGVNVQPYSCTSANFAVYTGLLLP 240

Query: 241 GDRIMGLDTPSGGNTSHGYCTPNGRKVSGASIFFESLPYKECVSPFGYCDVVTSTTHKSL 300
           GDRIMGLD+PSGG+ SHG+  P G+KVS ASIFFES PYKEC SPF YCD+VTSTTHKSL
Sbjct: 241 GDRIMGLDSPSGGHMSHGWYMPGGKKVSAASIFFESFPYKECASPFDYCDIVTSTTHKSL 300

Query: 301 RGPRGGIIFYRKGSKPRKRAMLLYQGNETDQYDFEEKINFAVFPSLQGGPHNNHIAALAV 360
           RGPRGGIIFYR+G+K RK+ M L  G+   QYDFEEKINFAVFPSLQGGPHNNHIAALA+
Sbjct: 301 RGPRGGIIFYRRGAKSRKQGMSLSNGDCGCQYDFEEKINFAVFPSLQGGPHNNHIAALAI 360

Query: 361 ALKQVATPEYKAYMQQVKKNAQALAAALLRKKYKLVTGGTDNHMILWDLRSLGLTGKNYE 420
           ALK VATPEYKAYMQQVKKNAQALA+AL+R+K KLVTGGTDNH++LWDL +LGLTGK YE
Sbjct: 361 ALKHVATPEYKAYMQQVKKNAQALASALVRRKCKLVTGGTDNHLLLWDLTTLGLTGKCYE 420

Query: 421 NVCEICHITLNKITIFGDNGIIIPGGVRV------------------------AAQIAIS 480
            VCE+CHITLNK  IFGDNG I PGGVR+                        AAQIA +
Sbjct: 421 KVCEMCHITLNKTAIFGDNGAICPGGVRIGSPAMTSRGCLESDFETMADFLYRAAQIASA 480

Query: 481 VQREYGKLPKTYLKGLLSNNEVVELRNRVESFSAQFAMPGFE 498
           VQR++GKL K +LKGL +N ++V+LRN+VE+F+AQFAMPGF+
Sbjct: 481 VQRDHGKLQKEFLKGLHNNKDIVDLRNQVEAFAAQFAMPGFD 508

BLAST of Cp4.1LG01g01850 vs. NCBI nr
Match: gi|944230153|gb|KQK94539.1| (hypothetical protein SETIT_026132mg [Setaria italica])

HSP 1 Score: 472.2 bits (1214), Expect = 1.1e-129
Identity = 233/400 (58.25%), Postives = 285/400 (71.25%), Query Frame = 1

Query: 123 VRSWGDQTLRVADPNLFGIMEKEKQRQFKGIELIASENYVCRAVMEALGSHLTNKYSEGM 182
           V +WG   L  ADP ++ ++E+EK+RQ +GIELIASEN+   AVMEALGS LTNKYSEGM
Sbjct: 135 VATWGLTPLAGADPEIYDLLEREKRRQRRGIELIASENFTSFAVMEALGSALTNKYSEGM 194

Query: 183 PGARYYCGNQYIDEIETLCRERALAAFGLSSDNWGVNVQPYSCTSANFAVYTGLLLPGDR 242
           PGARYY GN  IDEIE LCR RALAAF L   +WGVNVQPYS + ANFA YT LL P DR
Sbjct: 195 PGARYYGGNDVIDEIENLCRSRALAAFRLDPASWGVNVQPYSGSPANFAAYTALLNPHDR 254

Query: 243 IMGLDTPSGGNTSHGYCTPNGRKVSGASIFFESLPYKECVSPFGYCDVVTSTTHKSLRGP 302
           IMGLD PSGG+ +HGY T  G+K+S  SI+FESLPYKE  +PF YCDVVT+TTHKSLRGP
Sbjct: 255 IMGLDLPSGGHLTHGYYTSGGKKISATSIYFESLPYKEAANPFEYCDVVTTTTHKSLRGP 314

Query: 303 RGGIIFYRKGSKPRKRAMLLYQGNETDQYDFEEKINFAVFPSLQGGPHNNHIAALAVALK 362
           R G+IFYRKG KP K+        E   YD+E+KINFAVFPSLQGGPHN+ IAALAVAL+
Sbjct: 315 RAGMIFYRKGPKPPKKGQ-----PEGAVYDYEDKINFAVFPSLQGGPHNHQIAALAVALQ 374

Query: 363 QVATPEYKAYMQQVKKNAQALAAALLRKKYKLVTGGTDNHMILWDLRSLGLTGKNYENVC 422
           Q  TP +KAY +QVK NA A+   L+ K YK+VT GT+NH++LWDLR LGLTG   E +C
Sbjct: 375 QTMTPGFKAYAKQVKANAVAIGNYLMSKGYKMVTDGTENHLVLWDLRPLGLTGNKVEKLC 434

Query: 423 EICHITLNKITIFGDNGIIIPGGVRV------------------------AAQIAISVQR 482
           ++CHITLNK  +FGD+  + PGGVR+                        A  I +++Q+
Sbjct: 435 DLCHITLNKNAVFGDSSALSPGGVRIGAPAMTSRGLVEKDFEQIGEFLHRAVTICLNIQK 494

Query: 483 EYGKLPKTYLKGLLSNNEVVELRNRVESFSAQFAMPGFEI 499
           E+GKL K + KGL++N ++  L+  VE F+  F MPGF +
Sbjct: 495 EHGKLLKDFNKGLVNNKDIENLKAEVEKFADSFDMPGFTL 529

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
GLYC7_ARATH7.6e-9964.69Serine hydroxymethyltransferase 7 OS=Arabidopsis thaliana GN=SHM7 PE=2 SV=1[more]
GLYC6_ARATH1.6e-9664.55Serine hydroxymethyltransferase 6 OS=Arabidopsis thaliana GN=SHM6 PE=2 SV=1[more]
GLYC5_ARATH1.4e-6552.05Serine hydroxymethyltransferase 5 OS=Arabidopsis thaliana GN=SHM5 PE=3 SV=1[more]
GLYC4_ARATH2.5e-6551.65Serine hydroxymethyltransferase 4 OS=Arabidopsis thaliana GN=SHM4 PE=1 SV=1[more]
GLYA2_SULDN3.3e-5435.60Serine hydroxymethyltransferase 2 OS=Sulfurimonas denitrificans (strain ATCC 338... [more]
Match NameE-valueIdentityDescription
A0A0A0LAD1_CUCSA2.2e-20965.59Serine hydroxymethyltransferase OS=Cucumis sativus GN=Csa_3G221750 PE=3 SV=1[more]
I1KUN3_SOYBN1.1e-20873.09Serine hydroxymethyltransferase OS=Glycine max GN=GLYMA_08G187800 PE=3 SV=1[more]
A0A061FNN6_THECC8.0e-18866.09Serine hydroxymethyltransferase OS=Theobroma cacao GN=TCM_034999 PE=3 SV=1[more]
K3ZHZ4_SETIT7.6e-13058.25Serine hydroxymethyltransferase OS=Setaria italica GN=SETIT_026132mg PE=3 SV=1[more]
A9P855_POPTR3.1e-11548.50Serine hydroxymethyltransferase OS=Populus trichocarpa PE=2 SV=1[more]
Match NameE-valueIdentityDescription
AT1G36370.14.3e-10064.69 serine hydroxymethyltransferase 7[more]
AT1G22020.18.9e-9864.55 serine hydroxymethyltransferase 6[more]
AT4G13890.18.2e-6752.05 Pyridoxal phosphate (PLP)-dependent transferases superfamily protein[more]
AT4G13930.11.4e-6651.65 serine hydroxymethyltransferase 4[more]
AT4G32520.14.3e-5256.38 serine hydroxymethyltransferase 3[more]
Match NameE-valueIdentityDescription
gi|659112103|ref|XP_008456065.1|1.6e-21065.76PREDICTED: serine hydroxymethyltransferase 7-like [Cucumis melo][more]
gi|449457095|ref|XP_004146284.1|3.1e-20965.59PREDICTED: serine hydroxymethyltransferase 7-like [Cucumis sativus][more]
gi|947095479|gb|KRH44064.1|1.5e-20873.09hypothetical protein GLYMA_08G187800 [Glycine max][more]
gi|590598484|ref|XP_007018909.1|1.1e-18766.09Serine hydroxymethyltransferase 6 isoform 2 [Theobroma cacao][more]
gi|944230153|gb|KQK94539.1|1.1e-12958.25hypothetical protein SETIT_026132mg [Setaria italica][more]
The following terms have been associated with this gene:
Vocabulary: Molecular Function
TermDefinition
GO:0030170pyridoxal phosphate binding
GO:0004372glycine hydroxymethyltransferase activity
GO:0003824catalytic activity
GO:0016740transferase activity
Vocabulary: Biological Process
TermDefinition
GO:0006563L-serine metabolic process
GO:0006544glycine metabolic process
Vocabulary: INTERPRO
TermDefinition
IPR019798Ser_HO-MeTrfase_PLP_BS
IPR019775WD40_repeat_CS
IPR015424PyrdxlP-dep_Trfase
IPR015422PyrdxlP-dep_Trfase_dom1
IPR015421PyrdxlP-dep_Trfase_major
IPR001085Ser_HO-MeTrfase
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006544 glycine metabolic process
biological_process GO:0035999 tetrahydrofolate interconversion
biological_process GO:0006260 DNA replication
biological_process GO:0006281 DNA repair
biological_process GO:0006563 L-serine metabolic process
biological_process GO:0032508 DNA duplex unwinding
biological_process GO:0006310 DNA recombination
biological_process GO:0032259 methylation
biological_process GO:0006730 one-carbon metabolic process
biological_process GO:0019264 glycine biosynthetic process from serine
cellular_component GO:0005575 cellular_component
cellular_component GO:0005657 replication fork
molecular_function GO:0008168 methyltransferase activity
molecular_function GO:0030170 pyridoxal phosphate binding
molecular_function GO:0004372 glycine hydroxymethyltransferase activity
molecular_function GO:0005524 ATP binding
molecular_function GO:0043140 ATP-dependent 3'-5' DNA helicase activity
molecular_function GO:0003676 nucleic acid binding
molecular_function GO:0003824 catalytic activity
molecular_function GO:0016740 transferase activity

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG01g01850.1Cp4.1LG01g01850.1mRNA


Analysis Name: InterPro Annotations of Cucurbita pepo
Date Performed: 2017-12-02
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001085Serine hydroxymethyltransferasePANTHERPTHR11680SERINE HYDROXYMETHYLTRANSFERASEcoord: 99..498
score: 7.4E
IPR001085Serine hydroxymethyltransferasePFAMPF00464SHMTcoord: 133..279
score: 7.7E-58coord: 282..451
score: 1.4
IPR015421Pyridoxal phosphate-dependent transferase, major region, subdomain 1GENE3DG3DSA:3.40.640.10coord: 279..369
score: 2.3E-24coord: 160..264
score: 9.9
IPR015422Pyridoxal phosphate-dependent transferase, major region, subdomain 2GENE3DG3DSA:3.90.1150.10coord: 370..496
score: 2.5
IPR015424Pyridoxal phosphate-dependent transferaseunknownSSF53383PLP-dependent transferasescoord: 128..495
score: 5.38E
IPR019775WD40 repeat, conserved sitePROSITEPS00678WD_REPEATS_1coord: 394..408
scor
IPR019798Serine hydroxymethyltransferase, pyridoxal phosphate binding sitePROSITEPS00096SHMTcoord: 289..305
scor
NoneNo IPR availablePANTHERPTHR11680:SF7SERINE HYDROXYMETHYLTRANSFERASE 6-RELATEDcoord: 99..498
score: 7.4E