Csa3G221750 (gene) Cucumber (Chinese Long) v2

BLAST of Csa3G221750 vs. Swiss-Prot
Match: GLYC7_ARATH (Serine hydroxymethyltransferase 7 OS=Arabidopsis thaliana GN=SHM7 PE=2 SV=1)

HSP 1 Score: 766.1 bits (1977), Expect = 2.7e-220
Identity = 385/593 (64.92%), Postives = 455/593 (76.73%), Query Frame = 1

Query: 1   MDLSNSQSNISRG--------RVSPPHRTQIADDSILLHLDSSRRGPTYSAPPVPLQLME 60
           MDLS SQ+N   G          +P  R  IADDSI L +D S R    +  P+PLQL+E
Sbjct: 1   MDLSRSQTNFQLGFGCSHASMTPTPTPRAPIADDSINLQVDQSFRSLPTTFSPIPLQLLE 60

Query: 61  PQTENHRDENGDAKRDDDECDDRDVEEFRILGHSMCLKRRRDGESSSLLGSTKRVSC--- 120
            + E     +   K      D ++ E FRILGH MCLKR+RD     LL  +K       
Sbjct: 61  QKAEKTTTVDEPKKDGGGGGDQKEDEHFRILGHHMCLKRQRD--CPLLLTQSKHPKRSSI 120

Query: 121 -EFDMETRRSSVRSWGNQTLRAADPKLYGIMEKEKQRQINGIELIASENYVCRAVMEALG 180
            + D+E+RR++VR+WG+Q +  ADP ++ +MEKEKQRQ+ GIELIASEN+VCRAVMEALG
Sbjct: 121 GDSDLESRRAAVRAWGDQPIHLADPDIHELMEKEKQRQVRGIELIASENFVCRAVMEALG 180

Query: 181 SHLTNKYSEGMPGARYYGGNQYIDEIEILCRERALAAFDLNPHSWGVNVQPYSCTSANFA 240
           SHLTNKYSEGMPGARYY GNQYID+IE LC ERAL AF L    WGVNVQPYSCTSANFA
Sbjct: 181 SHLTNKYSEGMPGARYYTGNQYIDQIENLCIERALTAFGLESDKWGVNVQPYSCTSANFA 240

Query: 241 VYTGLLLPGDRIMALDSPSGGNPSHGYCTRNGKKVAGASIFFESFPYKVNPQTGYIDYDK 300
           VYTGLLLPG+RIM LDSPSGG+ SHGYCT  GKK++ ASIFFESFPYKVNPQTGYIDYDK
Sbjct: 241 VYTGLLLPGERIMGLDSPSGGHMSHGYCTPGGKKISAASIFFESFPYKVNPQTGYIDYDK 300

Query: 301 LEERALDFRPKLLICGGSAYPRELDYARFRQIADKCGAVLMCDMAQISGLVAAKECVSPF 360
           LE++ALD+RPK+LICGGS+YPR+ D+AR RQIADKCGAVLMCDMA ISGLVA KEC +PF
Sbjct: 301 LEDKALDYRPKILICGGSSYPRDWDFARVRQIADKCGAVLMCDMAHISGLVATKECSNPF 360

Query: 361 EYCDVVTSTTHKSLRGPRGGIIFYKKGYKPRGRAIVLYQGNDVKEYDFEERINFAVFPSL 420
           ++CD+VTSTTHK LRGPRGGIIFY++G K R +       +    YD EE+INFAVFPSL
Sbjct: 361 DHCDIVTSTTHKGLRGPRGGIIFYRRGPKIRKQGHHSSHCDTSTHYDLEEKINFAVFPSL 420

Query: 421 QGGPHNNHIAALAVALKQVATLEYKGYMQQVKKNAQALASALLRRNYKLVTGGTDNHMVL 480
           QGGPHNNHIAALA+ALKQVAT EYK Y+QQ+KKNAQALA+ALLRR  +LVTGGTDNH++L
Sbjct: 421 QGGPHNNHIAALAIALKQVATPEYKAYIQQMKKNAQALAAALLRRKCRLVTGGTDNHLLL 480

Query: 481 WDLRSLGLSGKNLEQLCEMCHITLNKIIISGDNGVITTGGVRIGTPAMTSRGCLESDFEL 540
           WDL  +GL+GK  E++CEMCHITLNK  I GDNG I+ GGVRIGTPAMT+RGC+ESDFE 
Sbjct: 481 WDLTPMGLTGKVYEKVCEMCHITLNKTAIFGDNGTISPGGVRIGTPAMTTRGCIESDFET 540

Query: 541 IVEFLHTAAQIAICIQREYGKMPNAFLTGLQSNKEVVELGNRVESFSAKFSMP 582
           + +FL  AAQI   +QRE+GK    F+  L +NK++ EL NRVE+F+ ++ MP
Sbjct: 541 MADFLIKAAQITSALQREHGKSHKEFVKSLCTNKDIAELRNRVEAFALQYEMP 591

BLAST of Csa3G221750 vs. Swiss-Prot
Match: GLYC6_ARATH (Serine hydroxymethyltransferase 6 OS=Arabidopsis thaliana GN=SHM6 PE=2 SV=1)

HSP 1 Score: 765.0 bits (1974), Expect = 6.0e-220
Identity = 380/575 (66.09%), Postives = 460/575 (80.00%), Query Frame = 1

Query: 18  PHRTQIADDSILLHLDSSRRGPTYSAPPVPLQLMEPQTE------NHRDENGDAKRDDDE 77
           P R  IADDSI L +DSS R  +   PPVPLQL+E + +         +E+ +   D+D+
Sbjct: 24  PPRIPIADDSITLQIDSSFRPSSNPMPPVPLQLLEQRFDVTGSCSRVVEEDDEVVGDNDD 83

Query: 78  CDDRDVEEFRILGHSMCLKRRRDGESSSLLGST--KRVSCEFDMETRRSSVRSWGNQTLR 137
            D R+ E+F +LGH M LKR R G S SL  S+  KR   +  +E+RR+ VR+WGNQ++ 
Sbjct: 84  DDQREEEQFILLGHPMKLKRGRGGNSYSLASSSPCKRFVVDSGIESRRAVVRAWGNQSIE 143

Query: 138 AADPKLYGIMEKEKQRQINGIELIASENYVCRAVMEALGSHLTNKYSEGMPGARYYGGNQ 197
            ADP+++  MEKEKQRQ  GIELIASEN+VCRAVMEALGSHLTNKYSEGMPGARYY GNQ
Sbjct: 144 EADPEIHEFMEKEKQRQFRGIELIASENFVCRAVMEALGSHLTNKYSEGMPGARYYTGNQ 203

Query: 198 YIDEIEILCRERALAAFDLNPHSWGVNVQPYSCTSANFAVYTGLLLPGDRIMALDSPSGG 257
           YID+IEILC+ERALAAF LN   WGVNVQPYSCTSANFAV+TGLL+PG+RIM LDSPSGG
Sbjct: 204 YIDQIEILCQERALAAFGLNHEKWGVNVQPYSCTSANFAVFTGLLMPGERIMGLDSPSGG 263

Query: 258 NPSHGYCTRNGKKVAGASIFFESFPYKVNPQTGYIDYDKLEERALDFRPKLLICGGSAYP 317
           + SHGY T  GKKV+GASIFFESFPYKV+P+TGYIDYDKLEE+ALD+RPK+LICGGS+YP
Sbjct: 264 HMSHGYYTPGGKKVSGASIFFESFPYKVDPRTGYIDYDKLEEKALDYRPKILICGGSSYP 323

Query: 318 RELDYARFRQIADKCGAVLMCDMAQISGLVAAKECVSPFEYCDVVTSTTHKSLRGPRGGI 377
           R+ ++ RFR IADKCGAVLM DMAQISGLVAAKE  +PF+YCD+VTSTTHKSLRGPRGGI
Sbjct: 324 RDWEFPRFRHIADKCGAVLMFDMAQISGLVAAKESPNPFDYCDIVTSTTHKSLRGPRGGI 383

Query: 378 IFYKKGYKPRGRAIVLYQGNDVKEYDFEERINFAVFPSLQGGPHNNHIAALAVALKQVAT 437
           IFYK+G KP+ ++I L       +YDFEE+INF+VFPSLQGGPHNNHIAALA+ALKQ A+
Sbjct: 384 IFYKRGLKPKKQSINLNHCESNIQYDFEEKINFSVFPSLQGGPHNNHIAALAIALKQAAS 443

Query: 438 LEYKGYMQQVKKNAQALASALLRRNYKLVTGGTDNHMVLWDLRSLGLSGKNLEQLCEMCH 497
            EYK YM+QVKKNA+ALASAL+ R  KL+TGGTDNH++LWDL  LGL+GK  E++CEMCH
Sbjct: 444 PEYKLYMRQVKKNAKALASALISRKCKLITGGTDNHLLLWDLTPLGLTGKVYEKVCEMCH 503

Query: 498 ITLNKIIISGDNGVITTGGVRIGTPAMTSRGCLESDFELIVEFLHTAAQIAICIQREYGK 557
           IT+NK+ I  +NGVI+ GGVRIG+PAMTSRGCLE +FE + +FL+ AAQIA   QRE+GK
Sbjct: 504 ITVNKVAIFSENGVISPGGVRIGSPAMTSRGCLEPEFETMADFLYRAAQIASAAQREHGK 563

Query: 558 MPNAFLTGLQSNKEVVELGNRVESFSAKFSMPGVE 585
           +    L  +   KE+ +L N+VE+F+ +F+MP  +
Sbjct: 564 LQKEPLKSIYHCKEIADLRNQVEAFATQFAMPAFD 598

BLAST of Csa3G221750 vs. Swiss-Prot
Match: GLYC4_ARATH (Serine hydroxymethyltransferase 4 OS=Arabidopsis thaliana GN=SHM4 PE=1 SV=1)

HSP 1 Score: 591.7 bits (1524), Expect = 9.1e-168
Identity = 293/463 (63.28%), Postives = 354/463 (76.46%), Query Frame = 1

Query: 120 VRSWGNQTLRAADPKLYGIMEKEKQRQINGIELIASENYVCRAVMEALGSHLTNKYSEGM 179
           V SWGN +L + DP+++ ++EKEK+RQ  GIELIASEN+   AV+EALGS LTNKYSEG+
Sbjct: 4   VSSWGNTSLVSVDPEIHDLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYSEGI 63

Query: 180 PGARYYGGNQYIDEIEILCRERALAAFDLNPHSWGVNVQPYSCTSANFAVYTGLLLPGDR 239
           PG RYYGGN++IDEIE LCR RAL AF  +P +WGVNVQPYS + ANFA YT LL P DR
Sbjct: 64  PGNRYYGGNEFIDEIENLCRSRALEAFHCDPAAWGVNVQPYSGSPANFAAYTALLQPHDR 123

Query: 240 IMALDSPSGGNPSHGYCTRNGKKVAGASIFFESFPYKVNPQTGYIDYDKLEERALDFRPK 299
           IM LD PSGG+ +HGY T  GKK++  SI+FES PYKVN  TGYIDYDKLEE+ALDFRPK
Sbjct: 124 IMGLDLPSGGHLTHGYYTSGGKKISATSIYFESLPYKVNFTTGYIDYDKLEEKALDFRPK 183

Query: 300 LLICGGSAYPRELDYARFRQIADKCGAVLMCDMAQISGLVAAKECVSPFEYCDVVTSTTH 359
           LLICGGSAYPR+ DYARFR IADK GA+L+CDMA ISGLVAA+E  +PFEYCDVVT+TTH
Sbjct: 184 LLICGGSAYPRDWDYARFRAIADKVGALLLCDMAHISGLVAAQEAANPFEYCDVVTTTTH 243

Query: 360 KSLRGPRGGIIFYKKGYKPRGRAIVLYQGNDVKEYDFEERINFAVFPSLQGGPHNNHIAA 419
           KSLRGPR G+IFY+KG KP  +        +   YDFE++INFAVFP+LQGGPHN+ I A
Sbjct: 244 KSLRGPRAGMIFYRKGPKPPKKG-----QPEGAVYDFEDKINFAVFPALQGGPHNHQIGA 303

Query: 420 LAVALKQVATLEYKGYMQQVKKNAQALASALLRRNYKLVTGGTDNHMVLWDLRSLGLSGK 479
           LAVALKQ  T  +K Y +QVK NA AL + L+ + Y++VT GT+NH+VLWDLR LGL+G 
Sbjct: 304 LAVALKQANTPGFKVYAKQVKANAVALGNYLMSKGYQIVTNGTENHLVLWDLRPLGLTGN 363

Query: 480 NLEQLCEMCHITLNKIIISGDNGVITTGGVRIGTPAMTSRGCLESDFELIVEFLHTAAQI 539
            +E+LC++C ITLNK  + GD+  +  GGVRIG PAMTSRG +E DFE I EFL  A  +
Sbjct: 364 KVEKLCDLCSITLNKNAVFGDSSALAPGGVRIGAPAMTSRGLVEKDFEQIGEFLSRAVTL 423

Query: 540 AICIQREYGKMPNAFLTGLQSNKEVVELGNRVESFSAKFSMPG 583
            + IQ+ YGK+   F  GL +NK++ +L   VE FSA + MPG
Sbjct: 424 TLDIQKTYGKLLKDFNKGLVNNKDLDQLKADVEKFSASYEMPG 461

BLAST of Csa3G221750 vs. Swiss-Prot
Match: GLYC5_ARATH (Serine hydroxymethyltransferase 5 OS=Arabidopsis thaliana GN=SHM5 PE=3 SV=1)

HSP 1 Score: 580.1 bits (1494), Expect = 2.7e-164
Identity = 285/463 (61.56%), Postives = 344/463 (74.30%), Query Frame = 1

Query: 120 VRSWGNQTLRAADPKLYGIMEKEKQRQINGIELIASENYVCRAVMEALGSHLTNKYSEGM 179
           V SWGN  L   DP++Y ++EKEK RQ  GIELIA+EN+   AVMEALGS LTNKYSEGM
Sbjct: 4   VYSWGNTHLDFVDPEIYDLIEKEKHRQCRGIELIAAENFTSVAVMEALGSCLTNKYSEGM 63

Query: 180 PGARYYGGNQYIDEIEILCRERALAAFDLNPHSWGVNVQPYSCTSANFAVYTGLLLPGDR 239
           PG RYYGG ++IDEIE LCR R+L AF  NP  WGVNVQPYS + ANFA YT LL P DR
Sbjct: 64  PGNRYYGGTEFIDEIESLCRSRSLEAFHCNPEKWGVNVQPYSGSPANFAAYTALLQPHDR 123

Query: 240 IMALDSPSGGNPSHGYCTRNGKKVAGASIFFESFPYKVNPQTGYIDYDKLEERALDFRPK 299
           IM LD PSGG+ +HGY +  GK ++  SI+FE+ PYKV+ +TGYIDYDKLEE+A+DFRPK
Sbjct: 124 IMGLDLPSGGHITHGYYSSGGKNISATSIYFENLPYKVDSKTGYIDYDKLEEKAMDFRPK 183

Query: 300 LLICGGSAYPRELDYARFRQIADKCGAVLMCDMAQISGLVAAKECVSPFEYCDVVTSTTH 359
           L+ICGG++YPRE DYARFR +ADK GA L+CDMA  S LVAA+E   PFEYCDVVT++TH
Sbjct: 184 LIICGGTSYPREWDYARFRAVADKVGAFLLCDMAHNSALVAAQEAADPFEYCDVVTTSTH 243

Query: 360 KSLRGPRGGIIFYKKGYKPRGRAIVLYQGNDVKEYDFEERINFAVFPSLQGGPHNNHIAA 419
           KSLRGPR G+IFY+KG KP  +        + + YDF+ +IN AVFP+LQ GPHNN I A
Sbjct: 244 KSLRGPRAGMIFYRKGPKPAKKG-----QPEGEVYDFDAKINSAVFPALQSGPHNNKIGA 303

Query: 420 LAVALKQVATLEYKGYMQQVKKNAQALASALLRRNYKLVTGGTDNHMVLWDLRSLGLSGK 479
           LAVALKQV    +K Y +QVK NA  LAS L+ + Y LVT GTDNH++LWDLR LGL+G 
Sbjct: 304 LAVALKQVMAPSFKVYAKQVKANAACLASYLINKGYTLVTDGTDNHLILWDLRPLGLTGN 363

Query: 480 NLEQLCEMCHITLNKIIISGDNGVITTGGVRIGTPAMTSRGCLESDFELIVEFLHTAAQI 539
            +E++CE+C+ITLN+  + GD   +  GGVRIGTPAMTSRG +E DFE I EFLH A  I
Sbjct: 364 KVEKVCELCYITLNRNAVFGDTSFLAPGGVRIGTPAMTSRGLVEKDFEKIGEFLHRAVTI 423

Query: 540 AICIQREYGKMPNAFLTGLQSNKEVVELGNRVESFSAKFSMPG 583
            + IQ +YGK+   F  GL +NKE+ E+   VE F+  F MPG
Sbjct: 424 TLDIQEQYGKVMKDFNKGLVNNKEIDEIKADVEEFTYDFDMPG 461

BLAST of Csa3G221750 vs. Swiss-Prot
Match: GLYC1_DICDI (Serine hydroxymethyltransferase 1 OS=Dictyostelium discoideum GN=shmt1 PE=1 SV=1)

HSP 1 Score: 521.9 bits (1343), Expect = 8.8e-147
Identity = 260/460 (56.52%), Postives = 332/460 (72.17%), Query Frame = 1

Query: 124 GNQTLRAADPKLYGIMEKEKQRQINGIELIASENYVCRAVMEALGSHLTNKYSEGMPGAR 183
           GN  L+  D +++ +M +EK RQ  G+ELIASEN+  RAVMEALGSH TNKY+EG PG+R
Sbjct: 6   GNTPLKEVDNEIFELMNREKDRQFKGLELIASENFTSRAVMEALGSHFTNKYAEGYPGSR 65

Query: 184 YYGGNQYIDEIEILCRERALAAFDLNPHSWGVNVQPYSCTSANFAVYTGLLLPGDRIMAL 243
           YYGG + +DE+E LC++RAL AF L+   WGVNVQPYS + ANFAVYT LL P DRIM L
Sbjct: 66  YYGGTEVVDELETLCQKRALKAFRLDESKWGVNVQPYSGSPANFAVYTALLRPHDRIMGL 125

Query: 244 DSPSGGNPSHGYCTRNGKKVAGASIFFESFPYKVNPQTGYIDYDKLEERALDFRPKLLIC 303
           D PSGG+ +HGY T + KK++ +SIFFES PY++    G IDY +LEE AL F+PKL+I 
Sbjct: 126 DLPSGGHLTHGYQT-DKKKISASSIFFESMPYQIGAD-GLIDYQRLEENALLFKPKLIIS 185

Query: 304 GGSAYPRELDYARFRQIADKCGAVLMCDMAQISGLVAAKECVSPFEYCDVVTSTTHKSLR 363
           G SAYPRE DY R R IADK GA LMCDMA  SGLVAA+   SPF+YCDVVTSTTHK+LR
Sbjct: 186 GASAYPREWDYKRMRAIADKVGAYLMCDMAHYSGLVAAQLLDSPFDYCDVVTSTTHKTLR 245

Query: 364 GPRGGIIFYKKGYKPRGRAIVLYQGNDVKEYDFEERINFAVFPSLQGGPHNNHIAALAVA 423
           GPR GIIF+++G +  G       G +++EYD E +INFAVFPSLQGGPH N IA +AVA
Sbjct: 246 GPRSGIIFFRRGKRVDGN------GKEIEEYDIESKINFAVFPSLQGGPHENVIAGVAVA 305

Query: 424 LKQVATLEYKGYMQQVKKNAQALASALLRRNYKLVTGGTDNHMVLWDLRSLGLSGKNLEQ 483
           LK+  + E+K Y  QVKKNA A+ +AL+ + YKLVT GTDNH++LWDLR   L+G   E+
Sbjct: 306 LKEADSQEFKEYALQVKKNAAAIGNALMNKGYKLVTNGTDNHLILWDLRPKELTGNKFEK 365

Query: 484 LCEMCHITLNKIIISGDNGVITTGGVRIGTPAMTSRGCLESDFELIVEFLHTAAQIAICI 543
             ++ +IT+NK  + GD   I+ GG+RIG+ A+TSRG  E+DFE I +FL     I++ I
Sbjct: 366 AADIANITVNKNAVHGDTNAISPGGIRIGSSALTSRGLKEADFEKIADFLDRIVSISLEI 425

Query: 544 QREYGKMPNAFLTGLQSNKEVVELGNRVESFSAKFSMPGV 584
           Q   GK    F+  +  +KE+++L   VE FS+KF++PG+
Sbjct: 426 QGRVGKKLVDFVVEINKSKELLDLRKEVEEFSSKFTLPGI 457

BLAST of Csa3G221750 vs. TrEMBL
Match: G7IPE1_MEDTR (Serine hydroxymethyltransferase OS=Medicago truncatula GN=MTR_2g006570 PE=3 SV=1)

HSP 1 Score: 867.1 bits (2239), Expect = 1.2e-248
Identity = 429/595 (72.10%), Postives = 498/595 (83.70%), Query Frame = 1

Query: 1   MDLSNSQSNIS----RGRVSPPHRTQIADDSILLHLDSSRRGPTYSAPPVPLQLMEPQTE 60
           MDLS+ QSN+S        SPP R  I DDSI LHL+SS R P+   P VPLQLMEPQTE
Sbjct: 1   MDLSHHQSNLSLNFSSSHASPPPRPPIPDDSIALHLESSFRDPSNPVPTVPLQLMEPQTE 60

Query: 61  NHRDENGDAKRDDDECDDRDVEEFRILGHSMCLKRRRDGESSSLLGSTKRVSCE------ 120
                  D + ++DE DDR+VEEFRILGHSMC+KRRRD ESS++  +TKR S E      
Sbjct: 61  KENGNAIDIESNNDE-DDREVEEFRILGHSMCIKRRRDSESSTI--TTKRASVESDRGDR 120

Query: 121 -FDMETRRSSVRSWGNQTLRAADPKLYGIMEKEKQRQINGIELIASENYVCRAVMEALGS 180
             D+E+R+++VRSWG+Q L+ ADP +Y I+EKEK+RQ  GIELIASEN+VCRAVMEALGS
Sbjct: 121 LLDLESRKAAVRSWGDQPLQVADPDIYEIIEKEKKRQFKGIELIASENFVCRAVMEALGS 180

Query: 181 HLTNKYSEGMPGARYYGGNQYIDEIEILCRERALAAFDLNPHSWGVNVQPYSCTSANFAV 240
           HLTNKYSEGMPGARYYGGNQYIDEIE LC ERALAAF+L+P  WGVNVQPYSCTSANFAV
Sbjct: 181 HLTNKYSEGMPGARYYGGNQYIDEIETLCCERALAAFNLDPKCWGVNVQPYSCTSANFAV 240

Query: 241 YTGLLLPGDRIMALDSPSGGNPSHGYCTRNGKKVAGASIFFESFPYKVNPQTGYIDYDKL 300
           YTGLL PGDRIM LD+PSGGN SHGY T NGKKV+GASIFFES  YK+NPQ+G+IDYDKL
Sbjct: 241 YTGLLAPGDRIMGLDTPSGGNTSHGYYTPNGKKVSGASIFFESLAYKINPQSGFIDYDKL 300

Query: 301 EERALDFRPKLLICGGSAYPRELDYARFRQIADKCGAVLMCDMAQISGLVAAKECVSPFE 360
           EERALDFRPK+LICGGS+YPRE DYARFR +ADKCGAVL+CDMAQISG++AAKECV+PF+
Sbjct: 301 EERALDFRPKILICGGSSYPREWDYARFRHVADKCGAVLLCDMAQISGIIAAKECVNPFD 360

Query: 361 YCDVVTSTTHKSLRGPRGGIIFYKKGYKPRGRAIVLYQGNDVKEYDFEERINFAVFPSLQ 420
           YCDVVTSTTHKSLRGPRGGIIFY+KG KPR R I+L QG++  +YDFEE+INFAVFPSLQ
Sbjct: 361 YCDVVTSTTHKSLRGPRGGIIFYRKGTKPRKRGILLTQGHESDQYDFEEKINFAVFPSLQ 420

Query: 421 GGPHNNHIAALAVALKQVATLEYKGYMQQVKKNAQALASALLRRNYKLVTGGTDNHMVLW 480
           GGPHNNHIAALA+ALKQVAT EYK YMQQVKKNAQALASALLRR  +LVTGGTDNH++LW
Sbjct: 421 GGPHNNHIAALAIALKQVATPEYKAYMQQVKKNAQALASALLRRKCRLVTGGTDNHLILW 480

Query: 481 DLRSLGLSGKNLEQLCEMCHITLNKIIISGDNGVITTGGVRIGTPAMTSRGCLESDFELI 540
           DLR LGL+GK  E++CE CHITLNKI I GDNG+I  GGVRIGTPAMTSRGCLE+DFE +
Sbjct: 481 DLRPLGLTGKFYEKVCEACHITLNKIAIFGDNGIIIPGGVRIGTPAMTSRGCLEADFETM 540

Query: 541 VEFLHTAAQIAICIQREYGKMPNAFLTGLQSNKEVVELGNRVESFSAKFSMPGVE 585
            +FL  AAQIA  +QRE+GK+       L+SN++++EL  RVE+F+ +F++PG +
Sbjct: 541 ADFLFRAAQIANMLQREHGKLQKTISKVLESNRDILELRARVEAFATQFALPGFD 592

BLAST of Csa3G221750 vs. TrEMBL
Match: F6H906_VITVI (Serine hydroxymethyltransferase OS=Vitis vinifera GN=VIT_12s0034g02380 PE=3 SV=1)

HSP 1 Score: 862.1 bits (2226), Expect = 4.0e-247
Identity = 434/584 (74.32%), Postives = 489/584 (83.73%), Query Frame = 1

Query: 1   MDLSNSQSNISRGRVSPPHRTQIADDSILLHLDSSRRGPTYSAPPVPLQLMEPQTENHRD 60
           MDL NS SN+S G  S     QIADDSI   +DSS R  T   P  PLQL+E   ENHR 
Sbjct: 1   MDL-NSSSNLSLG-FSSSSSVQIADDSIAFQIDSSFRESTNPMPTAPLQLLE---ENHRG 60

Query: 61  ENGDAKRDDDECDDRDVEEFRILGHSMCLKRRRDGESSSLLGSTKRVSCEFDMETRRSSV 120
           ENG    +  + ++  VEEFRILGHSMCLKRRRDGE +    + +R   E ++E RRS+V
Sbjct: 61  ENGGGGGESAD-EEEKVEEFRILGHSMCLKRRRDGEVTC--SAVRRDPMEAELEARRSAV 120

Query: 121 RSWGNQTLRAADPKLYGIMEKEKQRQINGIELIASENYVCRAVMEALGSHLTNKYSEGMP 180
           R+WGNQ L  ADP ++ IMEKEK+RQ  GIELIASEN+VCRAVMEALGSHLTNKYSEGMP
Sbjct: 121 RAWGNQRLCVADPDVFDIMEKEKRRQFKGIELIASENFVCRAVMEALGSHLTNKYSEGMP 180

Query: 181 GARYYGGNQYIDEIEILCRERALAAFDLNPHSWGVNVQPYSCTSANFAVYTGLLLPGDRI 240
           GARYY GNQYIDEIE LC +RAL AFDL+P +WGVNVQPYSCTSANFAVYTGLLLPGDRI
Sbjct: 181 GARYYCGNQYIDEIEWLCCKRALKAFDLDPENWGVNVQPYSCTSANFAVYTGLLLPGDRI 240

Query: 241 MALDSPSGGNPSHGYCTRNGKKVAGASIFFESFPYKVNPQTGYIDYDKLEERALDFRPKL 300
           M LD+PSGGN SHGY T NG+KV+GASIFFES PYKVNPQTGYID+DKLEERALDFRPK+
Sbjct: 241 MGLDTPSGGNTSHGYYTPNGRKVSGASIFFESLPYKVNPQTGYIDFDKLEERALDFRPKI 300

Query: 301 LICGGSAYPRELDYARFRQIADKCGAVLMCDMAQISGLVAAKECVSPFEYCDVVTSTTHK 360
           LICGGS+YPRE DYARFRQIADKCGAVL+CDMAQISGLVAAKECV+PF+YCD+VTSTTHK
Sbjct: 301 LICGGSSYPREWDYARFRQIADKCGAVLLCDMAQISGLVAAKECVNPFDYCDIVTSTTHK 360

Query: 361 SLRGPRGGIIFYKKGYKPRGRAIVLYQGNDVKEYDFEERINFAVFPSLQGGPHNNHIAAL 420
           SLRGPRGGIIFY+KG KPR R ++L QG+D   YD+EE+INFAVFPSLQGGPHNNHIAAL
Sbjct: 361 SLRGPRGGIIFYRKGTKPRKRGMILSQGDDNDHYDYEEKINFAVFPSLQGGPHNNHIAAL 420

Query: 421 AVALKQVATLEYKGYMQQVKKNAQALASALLRRNYKLVTGGTDNHMVLWDLRSLGLSGKN 480
           A+ALKQVAT EYK YM QVKKNAQALASALLRR  +LVTGGTDNH++LWDLR+LGL+GKN
Sbjct: 421 AIALKQVATPEYKAYMLQVKKNAQALASALLRRKCRLVTGGTDNHLLLWDLRTLGLTGKN 480

Query: 481 LEQLCEMCHITLNKIIISGDNGVITTGGVRIGTPAMTSRGCLESDFELIVEFLHTAAQIA 540
            E++CEMCHITLNKI I GDNG IT GGVRIGTPAMTSRGCLE+DFE I +FL  AAQIA
Sbjct: 481 YEKVCEMCHITLNKIAIFGDNGTITPGGVRIGTPAMTSRGCLEADFETIADFLLRAAQIA 540

Query: 541 ICIQREYGKMPNAFLTGLQSNKEVVELGNRVESFSAKFSMPGVE 585
             +QRE+GKM  AFL GL+SNK++VEL  RVE F+ +F MPG +
Sbjct: 541 SVVQREHGKMQKAFLKGLESNKDIVELRTRVEIFATQFVMPGFD 576

BLAST of Csa3G221750 vs. TrEMBL
Match: W9REX0_9ROSA (Serine hydroxymethyltransferase OS=Morus notabilis GN=L484_016722 PE=3 SV=1)

HSP 1 Score: 857.1 bits (2213), Expect = 1.3e-245
Identity = 439/595 (73.78%), Postives = 495/595 (83.19%), Query Frame = 1

Query: 1   MDLSNSQSNISRG---RVSPPHRTQIADDSILLHLDSSRRGPTYSAPPVPLQLMEPQTEN 60
           MDLS+ +S +S G   + SPP +TQIADDSI L LDSS R   YS  PVPLQL+EPQ E+
Sbjct: 1   MDLSHPRSTLSLGFSSQASPPTKTQIADDSIFLQLDSSLRDH-YSVTPVPLQLLEPQAEH 60

Query: 61  H-RDENGDAKR----DDDECDDRDVEEFRILGHSMCLKRRRDGESSS---LLGSTKRVSC 120
             R ENG+++R    D+DE DDR+VEEFRILGHSMCLKRRRD ESSS     G  KRVS 
Sbjct: 61  GMRTENGNSERTEPVDNDE-DDREVEEFRILGHSMCLKRRRDSESSSSSCTTGFPKRVSF 120

Query: 121 EFDMETRRSSVRSWGNQTLRAADPKLYGIMEKEKQRQINGIELIASENYVCRAVMEALGS 180
             D+E R ++VR WGNQ L  AD  ++ IMEKEK+RQ  GIELIASEN+VC+AVM+ALGS
Sbjct: 121 GPDLEARNAAVRMWGNQPLEVADRDVFEIMEKEKKRQFKGIELIASENFVCKAVMQALGS 180

Query: 181 HLTNKYSEGMPGARYYGGNQYIDEIEILCRERALAAFDLNPHSWGVNVQPYSCTSANFAV 240
           HLTNKYSEGMPGARYYGGNQYIDEIE LC ERALAAF+L+  +WGVNVQPYSCTSANFAV
Sbjct: 181 HLTNKYSEGMPGARYYGGNQYIDEIETLCWERALAAFNLDSENWGVNVQPYSCTSANFAV 240

Query: 241 YTGLLLPGDRIMALDSPSGGNPSHGYCTRNGKKVAGASIFFESFPYKVNPQTGYIDYDKL 300
           YTGLLLPGDRIM LD+PSGGN SHGY T NG+KV+GASIFFES PYKVNP TG IDYDKL
Sbjct: 241 YTGLLLPGDRIMGLDTPSGGNTSHGYYTPNGRKVSGASIFFESLPYKVNPLTGLIDYDKL 300

Query: 301 EERALDFRPKLLICGGSAYPRELDYARFRQIADKCGAVLMCDMAQISGLVAAKECVSPFE 360
           EERALDFRPK+LICGGS+YPRE DYARFR IADKCGAVLMCDMAQISG VAAK C +PF+
Sbjct: 301 EERALDFRPKILICGGSSYPREWDYARFRIIADKCGAVLMCDMAQISGFVAAKACDNPFD 360

Query: 361 YCDVVTSTTHKSLRGPRGGIIFYKKGYKPRGRAIVLYQGNDVKEYDFEERINFAVFPSLQ 420
           YCD+VTSTTHKSLRGPRGGIIFY+KG KPR R  VL  G++ + YDFEE+INFAVFP+LQ
Sbjct: 361 YCDIVTSTTHKSLRGPRGGIIFYRKGAKPRKRGAVL--GDESENYDFEEKINFAVFPALQ 420

Query: 421 GGPHNNHIAALAVALKQVATLEYKGYMQQVKKNAQALASALLRRNYKLVTGGTDNHMVLW 480
           GGPHNNHIAALA+ALKQVAT  YK YM QVKKNAQALASALLRR  +LVTGGTDNH++LW
Sbjct: 421 GGPHNNHIAALAIALKQVATPVYKAYMHQVKKNAQALASALLRRKCRLVTGGTDNHLLLW 480

Query: 481 DLRSLGLSGKNLEQLCEMCHITLNKIIISGDNGVITTGGVRIGTPAMTSRGCLESDFELI 540
           DLR LGL+GKN E++CEMCHITLNKI I GDNG IT GGVRIGTPAMTSRGCLESDFE I
Sbjct: 481 DLRPLGLTGKNYEKVCEMCHITLNKIAIFGDNGTITPGGVRIGTPAMTSRGCLESDFETI 540

Query: 541 VEFLHTAAQIAICIQREYGKMPNAFLTGLQSNKEVVELGNRVESFSAKFSMPGVE 585
            EFL  AA IA  + RE+GK    FL  L++NK++VEL  RVESF+++F+MPG +
Sbjct: 541 AEFLLKAAHIASAVLREHGKSQKLFLKALENNKDIVELRIRVESFASQFAMPGFD 591

BLAST of Csa3G221750 vs. TrEMBL
Match: A0A0R4J4J6_SOYBN (Serine hydroxymethyltransferase OS=Glycine max GN=GLYMA_12G170300 PE=3 SV=1)

HSP 1 Score: 848.6 bits (2191), Expect = 4.6e-243
Identity = 426/584 (72.95%), Postives = 482/584 (82.53%), Query Frame = 1

Query: 1   MDLSNSQSNISRGRVSPPHRTQIADDSILLHLDSSRRGPTYSAPPVPLQLMEPQTENHRD 60
           MDLS+ QSN+S G  S                 S    P  S PPVPLQLMEPQTEN   
Sbjct: 1   MDLSHPQSNLSLGFSS-----------------SHASPPPRSDPPVPLQLMEPQTENG-- 60

Query: 61  ENGDAKRDDDECDDRDVEEFRILGHSMCLKRRRDGESSSLLGSTKRVSCEFDMETRRSSV 120
            N D + DDDE  D++VEEFRILGHSMCLKRRRD +SSS   + KRVS E D++ R+++V
Sbjct: 61  -NLDVESDDDE--DKEVEEFRILGHSMCLKRRRDCDSSSSSSAAKRVSVEPDLDARKAAV 120

Query: 121 RSWGNQTLRAADPKLYGIMEKEKQRQINGIELIASENYVCRAVMEALGSHLTNKYSEGMP 180
           R+WG Q L  ADP ++ IMEKEK+RQ  GIELIASEN+VCRAVMEALGSHLTNKYSEGMP
Sbjct: 121 RAWGCQPLSIADPDVHEIMEKEKKRQFRGIELIASENFVCRAVMEALGSHLTNKYSEGMP 180

Query: 181 GARYYGGNQYIDEIEILCRERALAAFDLNPHSWGVNVQPYSCTSANFAVYTGLLLPGDRI 240
           GARYYGGNQYIDEIE LC ERAL AF L+P  WGVNVQPYSCTSANFAVYTGLLLPGDRI
Sbjct: 181 GARYYGGNQYIDEIETLCCERALNAFGLDPKCWGVNVQPYSCTSANFAVYTGLLLPGDRI 240

Query: 241 MALDSPSGGNPSHGYCTRNGKKVAGASIFFESFPYKVNPQTGYIDYDKLEERALDFRPKL 300
           M LD+PSGGN SHGY T NGKKV+GASIFFES PYKVNPQTGYIDYDKLEERALDFRPK+
Sbjct: 241 MGLDTPSGGNTSHGYYTPNGKKVSGASIFFESLPYKVNPQTGYIDYDKLEERALDFRPKI 300

Query: 301 LICGGSAYPRELDYARFRQIADKCGAVLMCDMAQISGLVAAKECVSPFEYCDVVTSTTHK 360
           LICGGS+YPRE DYARFR IADKCGAVL+CDMAQISG++AAKECV+PF+YCD+VTSTTHK
Sbjct: 301 LICGGSSYPREWDYARFRHIADKCGAVLLCDMAQISGIIAAKECVNPFDYCDIVTSTTHK 360

Query: 361 SLRGPRGGIIFYKKGYKPRGRAIVLYQGNDVKEYDFEERINFAVFPSLQGGPHNNHIAAL 420
           SLRGPRGGIIFY+KG KPR R I+L QG++  +YDFEE+INFAVFPS+QGGPHNNHIAAL
Sbjct: 361 SLRGPRGGIIFYRKGTKPRKRGILLSQGHESDQYDFEEKINFAVFPSMQGGPHNNHIAAL 420

Query: 421 AVALKQVATLEYKGYMQQVKKNAQALASALLRRNYKLVTGGTDNHMVLWDLRSLGLSGKN 480
           A+ALKQVAT EYK YMQQVKKNAQALA ALLRR  +LVTGGTDNH++LWDLR LGL+GK 
Sbjct: 421 AIALKQVATPEYKAYMQQVKKNAQALACALLRRKCRLVTGGTDNHLILWDLRPLGLTGKF 480

Query: 481 LEQLCEMCHITLNKIIISGDNGVITTGGVRIGTPAMTSRGCLESDFELIVEFLHTAAQIA 540
            E++CE CHITLNKI I GDNG I  GGVR+GTPAMTSRGCLE+ FE + EFL  AAQIA
Sbjct: 481 YEKVCETCHITLNKIAIFGDNGTIIPGGVRVGTPAMTSRGCLEAHFETMAEFLIRAAQIA 540

Query: 541 ICIQREYGKMPNAFLTGLQSNKEVVELGNRVESFSAKFSMPGVE 585
             +QRE+GK+    L GL+SN++VVEL  RVE+F+ +F+MPG +
Sbjct: 541 SILQREHGKLQKTTLKGLESNRDVVELRARVEAFATQFAMPGFD 562

BLAST of Csa3G221750 vs. TrEMBL
Match: A0A061E176_THECC (Serine hydroxymethyltransferase 6 isoform 1 OS=Theobroma cacao GN=TCM_006931 PE=3 SV=1)

HSP 1 Score: 845.9 bits (2184), Expect = 3.0e-242
Identity = 423/592 (71.45%), Postives = 492/592 (83.11%), Query Frame = 1

Query: 1   MDLSN----SQSNISRGRVSPP---HRTQIADDSILLHLDSSRRGPTYSAPPVPLQLMEP 60
           MDLS+    + S +S G VSP    HR+QI DDSI L LD+  R  T+  PPVPLQL+EP
Sbjct: 1   MDLSSQSTDTSSTLSLGLVSPSPPSHRSQIVDDSITLQLDT--RDTTHPVPPVPLQLLEP 60

Query: 61  QTENHRD-ENGDAKRDDDECDD---RDVEEFRILGHSMCLKRRRDGESSSLLGSTKRVSC 120
           QTENH   +NG  K  +   +D   +++EEF ILGH MCLKRRRD  SSS   STKR++ 
Sbjct: 61  QTENHNSLQNGKQKESESYNEDEESKEMEEFHILGHPMCLKRRRDSCSSSSSSSTKRMTV 120

Query: 121 EFDMETRRSSVRSWGNQTLRAADPKLYGIMEKEKQRQINGIELIASENYVCRAVMEALGS 180
           E D+ETR+++V+SWG+Q L  ADP ++ IME+EK+RQ  GIELIASEN+VCRAVM+ALGS
Sbjct: 121 EPDLETRKATVKSWGSQPLHVADPDVFDIMEQEKKRQFLGIELIASENFVCRAVMDALGS 180

Query: 181 HLTNKYSEGMPGARYYGGNQYIDEIEILCRERALAAFDLNPHSWGVNVQPYSCTSANFAV 240
           HLTNKYSEGMPGARYYGGNQYIDEIE LC +RAL AF L+  +WGVNVQPYSCTSANFAV
Sbjct: 181 HLTNKYSEGMPGARYYGGNQYIDEIETLCWKRALEAFGLDAENWGVNVQPYSCTSANFAV 240

Query: 241 YTGLLLPGDRIMALDSPSGGNPSHGYCTRNGKKVAGASIFFESFPYKVNPQTGYIDYDKL 300
           YTGLLLPGDRIM LD+PSGGN SHGY T NG+KV+GASIFFES PYKVNPQTGYIDY+KL
Sbjct: 241 YTGLLLPGDRIMGLDTPSGGNTSHGYYTPNGRKVSGASIFFESLPYKVNPQTGYIDYEKL 300

Query: 301 EERALDFRPKLLICGGSAYPRELDYARFRQIADKCGAVLMCDMAQISGLVAAKECVSPFE 360
           EERALDFRPK+LICGGS+YPRE DYARFRQIADKCGAVL+CDMAQISGL+AAKE  +PF+
Sbjct: 301 EERALDFRPKILICGGSSYPREWDYARFRQIADKCGAVLLCDMAQISGLIAAKESANPFD 360

Query: 361 YCDVVTSTTHKSLRGPRGGIIFYKKGYKPRGRAIVLYQGNDVKEYDFEERINFAVFPSLQ 420
           YCD+VTSTTHKSLRGPRGGIIFY+KG KPR   ++L QG+D  +YDFEE+INFAVFPSLQ
Sbjct: 361 YCDIVTSTTHKSLRGPRGGIIFYRKGTKPRKGGMLLSQGDDNDQYDFEEKINFAVFPSLQ 420

Query: 421 GGPHNNHIAALAVALKQVATLEYKGYMQQVKKNAQALASALLRRNYKLVTGGTDNHMVLW 480
           GGPHNNHIAALA+ALKQVAT EYK YM QVKKNAQALASAL RR  +LVTGGTDNH++LW
Sbjct: 421 GGPHNNHIAALAIALKQVATPEYKAYMHQVKKNAQALASALSRRKCRLVTGGTDNHLLLW 480

Query: 481 DLRSLGLSGKNLEQLCEMCHITLNKIIISGDNGVITTGGVRIGTPAMTSRGCLESDFELI 540
           DLR  GL+GK  E++CEMCHITLNKI I GDNG IT GGVRIGTPAMTSRGCLESDFE I
Sbjct: 481 DLRPFGLTGKIYEKVCEMCHITLNKIAIFGDNGAITPGGVRIGTPAMTSRGCLESDFETI 540

Query: 541 VEFLHTAAQIAICIQREYGKMPNAFLTGLQSNKEVVELGNRVESFSAKFSMP 582
            +FL  AA IA  +QR++GK+  A L  L +NK+++EL  +VE+F+++F+MP
Sbjct: 541 ADFLLRAAHIASMMQRDHGKLQKASLKSLHNNKDLLELRTQVETFASQFAMP 590

BLAST of Csa3G221750 vs. TAIR10
Match: AT1G36370.1 (AT1G36370.1 serine hydroxymethyltransferase 7)

HSP 1 Score: 766.1 bits (1977), Expect = 1.5e-221
Identity = 385/593 (64.92%), Postives = 455/593 (76.73%), Query Frame = 1

Query: 1   MDLSNSQSNISRG--------RVSPPHRTQIADDSILLHLDSSRRGPTYSAPPVPLQLME 60
           MDLS SQ+N   G          +P  R  IADDSI L +D S R    +  P+PLQL+E
Sbjct: 1   MDLSRSQTNFQLGFGCSHASMTPTPTPRAPIADDSINLQVDQSFRSLPTTFSPIPLQLLE 60

Query: 61  PQTENHRDENGDAKRDDDECDDRDVEEFRILGHSMCLKRRRDGESSSLLGSTKRVSC--- 120
            + E     +   K      D ++ E FRILGH MCLKR+RD     LL  +K       
Sbjct: 61  QKAEKTTTVDEPKKDGGGGGDQKEDEHFRILGHHMCLKRQRD--CPLLLTQSKHPKRSSI 120

Query: 121 -EFDMETRRSSVRSWGNQTLRAADPKLYGIMEKEKQRQINGIELIASENYVCRAVMEALG 180
            + D+E+RR++VR+WG+Q +  ADP ++ +MEKEKQRQ+ GIELIASEN+VCRAVMEALG
Sbjct: 121 GDSDLESRRAAVRAWGDQPIHLADPDIHELMEKEKQRQVRGIELIASENFVCRAVMEALG 180

Query: 181 SHLTNKYSEGMPGARYYGGNQYIDEIEILCRERALAAFDLNPHSWGVNVQPYSCTSANFA 240
           SHLTNKYSEGMPGARYY GNQYID+IE LC ERAL AF L    WGVNVQPYSCTSANFA
Sbjct: 181 SHLTNKYSEGMPGARYYTGNQYIDQIENLCIERALTAFGLESDKWGVNVQPYSCTSANFA 240

Query: 241 VYTGLLLPGDRIMALDSPSGGNPSHGYCTRNGKKVAGASIFFESFPYKVNPQTGYIDYDK 300
           VYTGLLLPG+RIM LDSPSGG+ SHGYCT  GKK++ ASIFFESFPYKVNPQTGYIDYDK
Sbjct: 241 VYTGLLLPGERIMGLDSPSGGHMSHGYCTPGGKKISAASIFFESFPYKVNPQTGYIDYDK 300

Query: 301 LEERALDFRPKLLICGGSAYPRELDYARFRQIADKCGAVLMCDMAQISGLVAAKECVSPF 360
           LE++ALD+RPK+LICGGS+YPR+ D+AR RQIADKCGAVLMCDMA ISGLVA KEC +PF
Sbjct: 301 LEDKALDYRPKILICGGSSYPRDWDFARVRQIADKCGAVLMCDMAHISGLVATKECSNPF 360

Query: 361 EYCDVVTSTTHKSLRGPRGGIIFYKKGYKPRGRAIVLYQGNDVKEYDFEERINFAVFPSL 420
           ++CD+VTSTTHK LRGPRGGIIFY++G K R +       +    YD EE+INFAVFPSL
Sbjct: 361 DHCDIVTSTTHKGLRGPRGGIIFYRRGPKIRKQGHHSSHCDTSTHYDLEEKINFAVFPSL 420

Query: 421 QGGPHNNHIAALAVALKQVATLEYKGYMQQVKKNAQALASALLRRNYKLVTGGTDNHMVL 480
           QGGPHNNHIAALA+ALKQVAT EYK Y+QQ+KKNAQALA+ALLRR  +LVTGGTDNH++L
Sbjct: 421 QGGPHNNHIAALAIALKQVATPEYKAYIQQMKKNAQALAAALLRRKCRLVTGGTDNHLLL 480

Query: 481 WDLRSLGLSGKNLEQLCEMCHITLNKIIISGDNGVITTGGVRIGTPAMTSRGCLESDFEL 540
           WDL  +GL+GK  E++CEMCHITLNK  I GDNG I+ GGVRIGTPAMT+RGC+ESDFE 
Sbjct: 481 WDLTPMGLTGKVYEKVCEMCHITLNKTAIFGDNGTISPGGVRIGTPAMTTRGCIESDFET 540

Query: 541 IVEFLHTAAQIAICIQREYGKMPNAFLTGLQSNKEVVELGNRVESFSAKFSMP 582
           + +FL  AAQI   +QRE+GK    F+  L +NK++ EL NRVE+F+ ++ MP
Sbjct: 541 MADFLIKAAQITSALQREHGKSHKEFVKSLCTNKDIAELRNRVEAFALQYEMP 591

BLAST of Csa3G221750 vs. TAIR10
Match: AT1G22020.1 (AT1G22020.1 serine hydroxymethyltransferase 6)

HSP 1 Score: 765.0 bits (1974), Expect = 3.4e-221
Identity = 380/575 (66.09%), Postives = 460/575 (80.00%), Query Frame = 1

Query: 18  PHRTQIADDSILLHLDSSRRGPTYSAPPVPLQLMEPQTE------NHRDENGDAKRDDDE 77
           P R  IADDSI L +DSS R  +   PPVPLQL+E + +         +E+ +   D+D+
Sbjct: 24  PPRIPIADDSITLQIDSSFRPSSNPMPPVPLQLLEQRFDVTGSCSRVVEEDDEVVGDNDD 83

Query: 78  CDDRDVEEFRILGHSMCLKRRRDGESSSLLGST--KRVSCEFDMETRRSSVRSWGNQTLR 137
            D R+ E+F +LGH M LKR R G S SL  S+  KR   +  +E+RR+ VR+WGNQ++ 
Sbjct: 84  DDQREEEQFILLGHPMKLKRGRGGNSYSLASSSPCKRFVVDSGIESRRAVVRAWGNQSIE 143

Query: 138 AADPKLYGIMEKEKQRQINGIELIASENYVCRAVMEALGSHLTNKYSEGMPGARYYGGNQ 197
            ADP+++  MEKEKQRQ  GIELIASEN+VCRAVMEALGSHLTNKYSEGMPGARYY GNQ
Sbjct: 144 EADPEIHEFMEKEKQRQFRGIELIASENFVCRAVMEALGSHLTNKYSEGMPGARYYTGNQ 203

Query: 198 YIDEIEILCRERALAAFDLNPHSWGVNVQPYSCTSANFAVYTGLLLPGDRIMALDSPSGG 257
           YID+IEILC+ERALAAF LN   WGVNVQPYSCTSANFAV+TGLL+PG+RIM LDSPSGG
Sbjct: 204 YIDQIEILCQERALAAFGLNHEKWGVNVQPYSCTSANFAVFTGLLMPGERIMGLDSPSGG 263

Query: 258 NPSHGYCTRNGKKVAGASIFFESFPYKVNPQTGYIDYDKLEERALDFRPKLLICGGSAYP 317
           + SHGY T  GKKV+GASIFFESFPYKV+P+TGYIDYDKLEE+ALD+RPK+LICGGS+YP
Sbjct: 264 HMSHGYYTPGGKKVSGASIFFESFPYKVDPRTGYIDYDKLEEKALDYRPKILICGGSSYP 323

Query: 318 RELDYARFRQIADKCGAVLMCDMAQISGLVAAKECVSPFEYCDVVTSTTHKSLRGPRGGI 377
           R+ ++ RFR IADKCGAVLM DMAQISGLVAAKE  +PF+YCD+VTSTTHKSLRGPRGGI
Sbjct: 324 RDWEFPRFRHIADKCGAVLMFDMAQISGLVAAKESPNPFDYCDIVTSTTHKSLRGPRGGI 383

Query: 378 IFYKKGYKPRGRAIVLYQGNDVKEYDFEERINFAVFPSLQGGPHNNHIAALAVALKQVAT 437
           IFYK+G KP+ ++I L       +YDFEE+INF+VFPSLQGGPHNNHIAALA+ALKQ A+
Sbjct: 384 IFYKRGLKPKKQSINLNHCESNIQYDFEEKINFSVFPSLQGGPHNNHIAALAIALKQAAS 443

Query: 438 LEYKGYMQQVKKNAQALASALLRRNYKLVTGGTDNHMVLWDLRSLGLSGKNLEQLCEMCH 497
            EYK YM+QVKKNA+ALASAL+ R  KL+TGGTDNH++LWDL  LGL+GK  E++CEMCH
Sbjct: 444 PEYKLYMRQVKKNAKALASALISRKCKLITGGTDNHLLLWDLTPLGLTGKVYEKVCEMCH 503

Query: 498 ITLNKIIISGDNGVITTGGVRIGTPAMTSRGCLESDFELIVEFLHTAAQIAICIQREYGK 557
           IT+NK+ I  +NGVI+ GGVRIG+PAMTSRGCLE +FE + +FL+ AAQIA   QRE+GK
Sbjct: 504 ITVNKVAIFSENGVISPGGVRIGSPAMTSRGCLEPEFETMADFLYRAAQIASAAQREHGK 563

Query: 558 MPNAFLTGLQSNKEVVELGNRVESFSAKFSMPGVE 585
           +    L  +   KE+ +L N+VE+F+ +F+MP  +
Sbjct: 564 LQKEPLKSIYHCKEIADLRNQVEAFATQFAMPAFD 598

BLAST of Csa3G221750 vs. TAIR10
Match: AT4G13930.1 (AT4G13930.1 serine hydroxymethyltransferase 4)

HSP 1 Score: 591.7 bits (1524), Expect = 5.1e-169
Identity = 293/463 (63.28%), Postives = 354/463 (76.46%), Query Frame = 1

Query: 120 VRSWGNQTLRAADPKLYGIMEKEKQRQINGIELIASENYVCRAVMEALGSHLTNKYSEGM 179
           V SWGN +L + DP+++ ++EKEK+RQ  GIELIASEN+   AV+EALGS LTNKYSEG+
Sbjct: 4   VSSWGNTSLVSVDPEIHDLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYSEGI 63

Query: 180 PGARYYGGNQYIDEIEILCRERALAAFDLNPHSWGVNVQPYSCTSANFAVYTGLLLPGDR 239
           PG RYYGGN++IDEIE LCR RAL AF  +P +WGVNVQPYS + ANFA YT LL P DR
Sbjct: 64  PGNRYYGGNEFIDEIENLCRSRALEAFHCDPAAWGVNVQPYSGSPANFAAYTALLQPHDR 123

Query: 240 IMALDSPSGGNPSHGYCTRNGKKVAGASIFFESFPYKVNPQTGYIDYDKLEERALDFRPK 299
           IM LD PSGG+ +HGY T  GKK++  SI+FES PYKVN  TGYIDYDKLEE+ALDFRPK
Sbjct: 124 IMGLDLPSGGHLTHGYYTSGGKKISATSIYFESLPYKVNFTTGYIDYDKLEEKALDFRPK 183

Query: 300 LLICGGSAYPRELDYARFRQIADKCGAVLMCDMAQISGLVAAKECVSPFEYCDVVTSTTH 359
           LLICGGSAYPR+ DYARFR IADK GA+L+CDMA ISGLVAA+E  +PFEYCDVVT+TTH
Sbjct: 184 LLICGGSAYPRDWDYARFRAIADKVGALLLCDMAHISGLVAAQEAANPFEYCDVVTTTTH 243

Query: 360 KSLRGPRGGIIFYKKGYKPRGRAIVLYQGNDVKEYDFEERINFAVFPSLQGGPHNNHIAA 419
           KSLRGPR G+IFY+KG KP  +        +   YDFE++INFAVFP+LQGGPHN+ I A
Sbjct: 244 KSLRGPRAGMIFYRKGPKPPKKG-----QPEGAVYDFEDKINFAVFPALQGGPHNHQIGA 303

Query: 420 LAVALKQVATLEYKGYMQQVKKNAQALASALLRRNYKLVTGGTDNHMVLWDLRSLGLSGK 479
           LAVALKQ  T  +K Y +QVK NA AL + L+ + Y++VT GT+NH+VLWDLR LGL+G 
Sbjct: 304 LAVALKQANTPGFKVYAKQVKANAVALGNYLMSKGYQIVTNGTENHLVLWDLRPLGLTGN 363

Query: 480 NLEQLCEMCHITLNKIIISGDNGVITTGGVRIGTPAMTSRGCLESDFELIVEFLHTAAQI 539
            +E+LC++C ITLNK  + GD+  +  GGVRIG PAMTSRG +E DFE I EFL  A  +
Sbjct: 364 KVEKLCDLCSITLNKNAVFGDSSALAPGGVRIGAPAMTSRGLVEKDFEQIGEFLSRAVTL 423

Query: 540 AICIQREYGKMPNAFLTGLQSNKEVVELGNRVESFSAKFSMPG 583
            + IQ+ YGK+   F  GL +NK++ +L   VE FSA + MPG
Sbjct: 424 TLDIQKTYGKLLKDFNKGLVNNKDLDQLKADVEKFSASYEMPG 461

BLAST of Csa3G221750 vs. TAIR10
Match: AT4G13890.1 (AT4G13890.1 Pyridoxal phosphate (PLP)-dependent transferases superfamily protein)

HSP 1 Score: 580.1 bits (1494), Expect = 1.5e-165
Identity = 285/463 (61.56%), Postives = 344/463 (74.30%), Query Frame = 1

Query: 120 VRSWGNQTLRAADPKLYGIMEKEKQRQINGIELIASENYVCRAVMEALGSHLTNKYSEGM 179
           V SWGN  L   DP++Y ++EKEK RQ  GIELIA+EN+   AVMEALGS LTNKYSEGM
Sbjct: 4   VYSWGNTHLDFVDPEIYDLIEKEKHRQCRGIELIAAENFTSVAVMEALGSCLTNKYSEGM 63

Query: 180 PGARYYGGNQYIDEIEILCRERALAAFDLNPHSWGVNVQPYSCTSANFAVYTGLLLPGDR 239
           PG RYYGG ++IDEIE LCR R+L AF  NP  WGVNVQPYS + ANFA YT LL P DR
Sbjct: 64  PGNRYYGGTEFIDEIESLCRSRSLEAFHCNPEKWGVNVQPYSGSPANFAAYTALLQPHDR 123

Query: 240 IMALDSPSGGNPSHGYCTRNGKKVAGASIFFESFPYKVNPQTGYIDYDKLEERALDFRPK 299
           IM LD PSGG+ +HGY +  GK ++  SI+FE+ PYKV+ +TGYIDYDKLEE+A+DFRPK
Sbjct: 124 IMGLDLPSGGHITHGYYSSGGKNISATSIYFENLPYKVDSKTGYIDYDKLEEKAMDFRPK 183

Query: 300 LLICGGSAYPRELDYARFRQIADKCGAVLMCDMAQISGLVAAKECVSPFEYCDVVTSTTH 359
           L+ICGG++YPRE DYARFR +ADK GA L+CDMA  S LVAA+E   PFEYCDVVT++TH
Sbjct: 184 LIICGGTSYPREWDYARFRAVADKVGAFLLCDMAHNSALVAAQEAADPFEYCDVVTTSTH 243

Query: 360 KSLRGPRGGIIFYKKGYKPRGRAIVLYQGNDVKEYDFEERINFAVFPSLQGGPHNNHIAA 419
           KSLRGPR G+IFY+KG KP  +        + + YDF+ +IN AVFP+LQ GPHNN I A
Sbjct: 244 KSLRGPRAGMIFYRKGPKPAKKG-----QPEGEVYDFDAKINSAVFPALQSGPHNNKIGA 303

Query: 420 LAVALKQVATLEYKGYMQQVKKNAQALASALLRRNYKLVTGGTDNHMVLWDLRSLGLSGK 479
           LAVALKQV    +K Y +QVK NA  LAS L+ + Y LVT GTDNH++LWDLR LGL+G 
Sbjct: 304 LAVALKQVMAPSFKVYAKQVKANAACLASYLINKGYTLVTDGTDNHLILWDLRPLGLTGN 363

Query: 480 NLEQLCEMCHITLNKIIISGDNGVITTGGVRIGTPAMTSRGCLESDFELIVEFLHTAAQI 539
            +E++CE+C+ITLN+  + GD   +  GGVRIGTPAMTSRG +E DFE I EFLH A  I
Sbjct: 364 KVEKVCELCYITLNRNAVFGDTSFLAPGGVRIGTPAMTSRGLVEKDFEKIGEFLHRAVTI 423

Query: 540 AICIQREYGKMPNAFLTGLQSNKEVVELGNRVESFSAKFSMPG 583
            + IQ +YGK+   F  GL +NKE+ E+   VE F+  F MPG
Sbjct: 424 TLDIQEQYGKVMKDFNKGLVNNKEIDEIKADVEEFTYDFDMPG 461

BLAST of Csa3G221750 vs. TAIR10
Match: AT4G37930.1 (AT4G37930.1 serine transhydroxymethyltransferase 1)

HSP 1 Score: 468.8 bits (1205), Expect = 5.0e-132
Identity = 243/465 (52.26%), Postives = 317/465 (68.17%), Query Frame = 1

Query: 125 NQTLRAADPKLYGIMEKEKQRQINGIELIASENYVCRAVMEALGSHLTNKYSEGMPGARY 184
           N  L   DP++  I+E EK RQ  G+ELI SEN+   +VM+A+GS +TNKYSEG PGARY
Sbjct: 52  NAPLEEVDPEIADIIEHEKARQWKGLELIPSENFTSVSVMQAVGSVMTNKYSEGYPGARY 111

Query: 185 YGGNQYIDEIEILCRERALAAFDLNPHSWGVNVQPYSCTSANFAVYTGLLLPGDRIMALD 244
           YGGN+YID  E LC++RAL AF L+P  WGVNVQP S + ANF VYT LL P +RIMALD
Sbjct: 112 YGGNEYIDMAETLCQKRALEAFRLDPEKWGVNVQPLSGSPANFHVYTALLKPHERIMALD 171

Query: 245 SPSGGNPSHGYCTRNGKKVAGASIFFESFPYKVNPQTGYIDYDKLEERALDFRPKLLICG 304
            P GG+ SHGY T + KK++  SIFFE+ PY+++  TGYIDYD++E+ A  FRPKL++ G
Sbjct: 172 LPHGGHLSHGYQT-DTKKISAVSIFFETMPYRLDESTGYIDYDQMEKSATLFRPKLIVAG 231

Query: 305 GSAYPRELDYARFRQIADKCGAVLMCDMAQISGLVAAKECVSPFEYCDVVTSTTHKSLRG 364
            SAY R  DYAR R++ +K  AV++ DMA ISGLVAA    SPF+Y DVVT+TTHKSLRG
Sbjct: 232 ASAYARLYDYARIRKVCNKQKAVMLADMAHISGLVAANVIPSPFDYADVVTTTTHKSLRG 291

Query: 365 PRGGIIFYKKGYKPRGRAIVLYQGNDVKEYDFEERINFAVFPSLQGGPHNNHIAALAVAL 424
           PRG +IF++KG K   +     QG +V  YDFE++IN AVFP LQGGPHN+ I  LAVAL
Sbjct: 292 PRGAMIFFRKGVKEINK-----QGKEVL-YDFEDKINQAVFPGLQGGPHNHTITGLAVAL 351

Query: 425 KQVATLEYKGYMQQVKKNAQALASALLRRNYKLVTGGTDNHMVLWDLRSLGLSGKNLEQL 484
           KQ  T EYK Y +QV  N+   A  L+ R Y+LV+GGTDNH+VL +L+  G+ G  +E++
Sbjct: 352 KQATTSEYKAYQEQVLSNSAKFAQTLMERGYELVSGGTDNHLVLVNLKPKGIDGSRVEKV 411

Query: 485 CEMCHITLNKIIISGDNGVITTGGVRIGTPAMTSRGCLESDFELIVEFLHTAAQIAICIQ 544
            E  HI  NK  + GD   +  GG+R+GTPA+TSRG +E DF  + E+   A  IA+ ++
Sbjct: 412 LEAVHIASNKNTVPGDVSAMVPGGIRMGTPALTSRGFVEEDFAKVAEYFDKAVTIALKVK 471

Query: 545 RE-YGKMPNAFLTGLQSN----KEVVELGNRVESFSAKFSMPGVE 585
            E  G     F++ ++S+     E+ +L + VE F+ +F   G E
Sbjct: 472 SEAQGTKLKDFVSAMESSSTIQSEIAKLRHEVEEFAKQFPTIGFE 509

BLAST of Csa3G221750 vs. NCBI nr
Match: gi|449457095|ref|XP_004146284.1| (PREDICTED: serine hydroxymethyltransferase 7-like [Cucumis sativus])

HSP 1 Score: 1187.9 bits (3072), Expect = 0.0e+00
Identity = 585/585 (100.00%), Postives = 585/585 (100.00%), Query Frame = 1

Query: 1   MDLSNSQSNISRGRVSPPHRTQIADDSILLHLDSSRRGPTYSAPPVPLQLMEPQTENHRD 60
           MDLSNSQSNISRGRVSPPHRTQIADDSILLHLDSSRRGPTYSAPPVPLQLMEPQTENHRD
Sbjct: 1   MDLSNSQSNISRGRVSPPHRTQIADDSILLHLDSSRRGPTYSAPPVPLQLMEPQTENHRD 60

Query: 61  ENGDAKRDDDECDDRDVEEFRILGHSMCLKRRRDGESSSLLGSTKRVSCEFDMETRRSSV 120
           ENGDAKRDDDECDDRDVEEFRILGHSMCLKRRRDGESSSLLGSTKRVSCEFDMETRRSSV
Sbjct: 61  ENGDAKRDDDECDDRDVEEFRILGHSMCLKRRRDGESSSLLGSTKRVSCEFDMETRRSSV 120

Query: 121 RSWGNQTLRAADPKLYGIMEKEKQRQINGIELIASENYVCRAVMEALGSHLTNKYSEGMP 180
           RSWGNQTLRAADPKLYGIMEKEKQRQINGIELIASENYVCRAVMEALGSHLTNKYSEGMP
Sbjct: 121 RSWGNQTLRAADPKLYGIMEKEKQRQINGIELIASENYVCRAVMEALGSHLTNKYSEGMP 180

Query: 181 GARYYGGNQYIDEIEILCRERALAAFDLNPHSWGVNVQPYSCTSANFAVYTGLLLPGDRI 240
           GARYYGGNQYIDEIEILCRERALAAFDLNPHSWGVNVQPYSCTSANFAVYTGLLLPGDRI
Sbjct: 181 GARYYGGNQYIDEIEILCRERALAAFDLNPHSWGVNVQPYSCTSANFAVYTGLLLPGDRI 240

Query: 241 MALDSPSGGNPSHGYCTRNGKKVAGASIFFESFPYKVNPQTGYIDYDKLEERALDFRPKL 300
           MALDSPSGGNPSHGYCTRNGKKVAGASIFFESFPYKVNPQTGYIDYDKLEERALDFRPKL
Sbjct: 241 MALDSPSGGNPSHGYCTRNGKKVAGASIFFESFPYKVNPQTGYIDYDKLEERALDFRPKL 300

Query: 301 LICGGSAYPRELDYARFRQIADKCGAVLMCDMAQISGLVAAKECVSPFEYCDVVTSTTHK 360
           LICGGSAYPRELDYARFRQIADKCGAVLMCDMAQISGLVAAKECVSPFEYCDVVTSTTHK
Sbjct: 301 LICGGSAYPRELDYARFRQIADKCGAVLMCDMAQISGLVAAKECVSPFEYCDVVTSTTHK 360

Query: 361 SLRGPRGGIIFYKKGYKPRGRAIVLYQGNDVKEYDFEERINFAVFPSLQGGPHNNHIAAL 420
           SLRGPRGGIIFYKKGYKPRGRAIVLYQGNDVKEYDFEERINFAVFPSLQGGPHNNHIAAL
Sbjct: 361 SLRGPRGGIIFYKKGYKPRGRAIVLYQGNDVKEYDFEERINFAVFPSLQGGPHNNHIAAL 420

Query: 421 AVALKQVATLEYKGYMQQVKKNAQALASALLRRNYKLVTGGTDNHMVLWDLRSLGLSGKN 480
           AVALKQVATLEYKGYMQQVKKNAQALASALLRRNYKLVTGGTDNHMVLWDLRSLGLSGKN
Sbjct: 421 AVALKQVATLEYKGYMQQVKKNAQALASALLRRNYKLVTGGTDNHMVLWDLRSLGLSGKN 480

Query: 481 LEQLCEMCHITLNKIIISGDNGVITTGGVRIGTPAMTSRGCLESDFELIVEFLHTAAQIA 540
           LEQLCEMCHITLNKIIISGDNGVITTGGVRIGTPAMTSRGCLESDFELIVEFLHTAAQIA
Sbjct: 481 LEQLCEMCHITLNKIIISGDNGVITTGGVRIGTPAMTSRGCLESDFELIVEFLHTAAQIA 540

Query: 541 ICIQREYGKMPNAFLTGLQSNKEVVELGNRVESFSAKFSMPGVET 586
           ICIQREYGKMPNAFLTGLQSNKEVVELGNRVESFSAKFSMPGVET
Sbjct: 541 ICIQREYGKMPNAFLTGLQSNKEVVELGNRVESFSAKFSMPGVET 585

BLAST of Csa3G221750 vs. NCBI nr
Match: gi|1012251186|ref|XP_015943653.1| (PREDICTED: serine hydroxymethyltransferase 6-like [Arachis duranensis])

HSP 1 Score: 880.2 bits (2273), Expect = 2.0e-252
Identity = 443/596 (74.33%), Postives = 498/596 (83.56%), Query Frame = 1

Query: 1   MDLSNSQSNISRG----RVSPPHRTQIADDSILLHLDSSRRGPTYSAPPVPLQLMEPQTE 60
           MDLS+  SN+S G      SPP R  I D+SI L L+SS R P++  PPVPLQL+EPQT 
Sbjct: 1   MDLSHPHSNLSLGFSSSHASPPRRPPIPDESITLQLESSLRDPSHPLPPVPLQLLEPQTA 60

Query: 61  ----NHRDENGDAKRDDDECDDRDVEEFRILGHSMCLKRRRD--GESSSLLGSTKRVSC- 120
               NH  E  DA  D+D   DRDVEEFRILGHSMCLKRRRD    SSS + S+KRVS  
Sbjct: 61  TDNGNHDSEFNDAADDED---DRDVEEFRILGHSMCLKRRRDCDSSSSSSISSSKRVSVS 120

Query: 121 -EFDMETRRSSVRSWGNQTLRAADPKLYGIMEKEKQRQINGIELIASENYVCRAVMEALG 180
            E D+E RR+ VRSWGNQ+L+ ADP ++ IMEKEK+RQ  GIELIASEN+VC+AVMEALG
Sbjct: 121 HESDLEARRALVRSWGNQSLQLADPDVFDIMEKEKRRQYRGIELIASENFVCKAVMEALG 180

Query: 181 SHLTNKYSEGMPGARYYGGNQYIDEIEILCRERALAAFDLNPHSWGVNVQPYSCTSANFA 240
           SHLTNKYSEGMPGARYYGGNQYIDEIE LC ERAL AF+L+P  WGVNVQPYSCTSANFA
Sbjct: 181 SHLTNKYSEGMPGARYYGGNQYIDEIETLCCERALLAFNLDPKCWGVNVQPYSCTSANFA 240

Query: 241 VYTGLLLPGDRIMALDSPSGGNPSHGYCTRNGKKVAGASIFFESFPYKVNPQTGYIDYDK 300
           VYTGLLLPGDRIM LD+PSGGN SHGY T NGKKV+GASIFFES PYKVNPQTGY+DY+K
Sbjct: 241 VYTGLLLPGDRIMGLDTPSGGNTSHGYYTPNGKKVSGASIFFESLPYKVNPQTGYVDYEK 300

Query: 301 LEERALDFRPKLLICGGSAYPRELDYARFRQIADKCGAVLMCDMAQISGLVAAKECVSPF 360
           LEERALDFRPK+LICGGS+YPRE DYARFR IADKCGAVLMCDMAQISGL+AAKEC +PF
Sbjct: 301 LEERALDFRPKILICGGSSYPREWDYARFRHIADKCGAVLMCDMAQISGLIAAKECSNPF 360

Query: 361 EYCDVVTSTTHKSLRGPRGGIIFYKKGYKPRGRAIVLYQGNDVKEYDFEERINFAVFPSL 420
           +YCD+VTSTTHKSLRGPRGGIIFY+KG KPR R I+L QGN+  +YDFEE+INFAVFPSL
Sbjct: 361 DYCDIVTSTTHKSLRGPRGGIIFYRKGAKPRKRGILLSQGNESDQYDFEEKINFAVFPSL 420

Query: 421 QGGPHNNHIAALAVALKQVATLEYKGYMQQVKKNAQALASALLRRNYKLVTGGTDNHMVL 480
           QGGPHNNHIAALA+ALKQVAT EYK YM QVKKNAQALASALLRR  +LVTGGTDNH+VL
Sbjct: 421 QGGPHNNHIAALAIALKQVATPEYKAYMHQVKKNAQALASALLRRKCRLVTGGTDNHLVL 480

Query: 481 WDLRSLGLSGKNLEQLCEMCHITLNKIIISGDNGVITTGGVRIGTPAMTSRGCLESDFEL 540
           WDLR LGL+GK  E++CE CHITLNKI I GDNGVI  GGVRIGTPAMTSRGCLE+DFE 
Sbjct: 481 WDLRPLGLTGKFYEKVCEACHITLNKIAIFGDNGVIIPGGVRIGTPAMTSRGCLEADFET 540

Query: 541 IVEFLHTAAQIAICIQREYGKMPNAFLTGLQSNKEVVELGNRVESFSAKFSMPGVE 585
           + EFL  AAQ+A  +QRE+GKM    L GL S++EVV+L  RVE+F+ +F+MPG +
Sbjct: 541 MAEFLFRAAQLANVLQREHGKMQKTVLKGLDSSREVVDLRARVEAFATQFAMPGFD 593

BLAST of Csa3G221750 vs. NCBI nr
Match: gi|1009124262|ref|XP_015878971.1| (PREDICTED: serine hydroxymethyltransferase 7-like [Ziziphus jujuba])

HSP 1 Score: 878.2 bits (2268), Expect = 7.7e-252
Identity = 432/596 (72.48%), Postives = 503/596 (84.40%), Query Frame = 1

Query: 1   MDLSNSQSNISRG---RVSPPHRTQIADDSILLHLDSSRRGPTYSAPPVPLQLMEPQTEN 60
           MDLS+ QSN+S G     SPPHRTQIADDSI L LDSS R PT+S  P+PLQL+EPQ E 
Sbjct: 1   MDLSHPQSNLSLGFSSHASPPHRTQIADDSISLQLDSSLRDPTHSVTPIPLQLLEPQAEC 60

Query: 61  HRDENGDAKRD-----DDECDDRDVEEFRILGHSMCLKRRRDGESSSLLGS----TKRVS 120
            R ENG+  RD     DD+ +DR+VEEFRILGHSMCLKRRRD ESSS   S    +KRVS
Sbjct: 61  QRTENGNLDRDMNDDDDDDDEDREVEEFRILGHSMCLKRRRDSESSSSSASATSNSKRVS 120

Query: 121 CEFDMETRRSSVRSWGNQTLRAADPKLYGIMEKEKQRQINGIELIASENYVCRAVMEALG 180
              D+E RRS+VR WG+Q LR ADP ++ IMEKEK+RQ  GIELIASEN+VC+AVMEALG
Sbjct: 121 FGPDLEARRSAVRLWGDQPLRIADPDVFHIMEKEKRRQFKGIELIASENFVCKAVMEALG 180

Query: 181 SHLTNKYSEGMPGARYYGGNQYIDEIEILCRERALAAFDLNPHSWGVNVQPYSCTSANFA 240
           SHLTNKYSEGMPGARYY GNQ+IDEIE LC ERALAAF+L+  +WGVNVQPYSCTSANFA
Sbjct: 181 SHLTNKYSEGMPGARYYCGNQHIDEIETLCCERALAAFNLDSENWGVNVQPYSCTSANFA 240

Query: 241 VYTGLLLPGDRIMALDSPSGGNPSHGYCTRNGKKVAGASIFFESFPYKVNPQTGYIDYDK 300
           VYTGLLLPGDRIM LD+P GGN SHGY   NG+KV+GASIFFES  YKVNPQTGYIDY+K
Sbjct: 241 VYTGLLLPGDRIMGLDTPCGGNTSHGYYLPNGRKVSGASIFFESLSYKVNPQTGYIDYEK 300

Query: 301 LEERALDFRPKLLICGGSAYPRELDYARFRQIADKCGAVLMCDMAQISGLVAAKECVSPF 360
           LEERALDFRPK+LICGGS+YPRE +Y+RFRQIADKCGAVLMCDMAQISGL++AKEC +PF
Sbjct: 301 LEERALDFRPKILICGGSSYPREWEYSRFRQIADKCGAVLMCDMAQISGLISAKECANPF 360

Query: 361 EYCDVVTSTTHKSLRGPRGGIIFYKKGYKPRGRAIVLYQGNDVKEYDFEERINFAVFPSL 420
           EYCD+VTSTTHKSLRGPRGGIIF++KG KPR R I+L QG++ ++YDFEE+INFAVFP++
Sbjct: 361 EYCDIVTSTTHKSLRGPRGGIIFFRKGTKPRKRGILLNQGDESEQYDFEEKINFAVFPAM 420

Query: 421 QGGPHNNHIAALAVALKQVATLEYKGYMQQVKKNAQALASALLRRNYKLVTGGTDNHMVL 480
           QGGPHNNHIAALA+ALKQVAT EYK YM QVKKNAQALASAL R+N++LVTGGTDNH+++
Sbjct: 421 QGGPHNNHIAALAIALKQVATPEYKAYMHQVKKNAQALASALKRKNFRLVTGGTDNHLLI 480

Query: 481 WDLRSLGLSGKNLEQLCEMCHITLNKIIISGDNGVITTGGVRIGTPAMTSRGCLESDFEL 540
           WDLR LGL+GK  E++CEMCHITLNKI I GDNG I   GVRIGTPAMTSRGC+E DFE 
Sbjct: 481 WDLRPLGLTGKIYEKVCEMCHITLNKIAIFGDNGTIVPRGVRIGTPAMTSRGCVEGDFET 540

Query: 541 IVEFLHTAAQIAICIQREYGKMPNAFLTGLQSNKEVVELGNRVESFSAKFSMPGVE 585
           I EFL+ AAQIA  + RE+G    A+L  L++NK++V+L  RVE+F+++F++PG +
Sbjct: 541 IAEFLYRAAQIATAVHREHGNQQKAYLKALENNKDIVDLRMRVETFASQFALPGFD 596

BLAST of Csa3G221750 vs. NCBI nr
Match: gi|1021582290|ref|XP_016180536.1| (PREDICTED: serine hydroxymethyltransferase 6-like [Arachis ipaensis])

HSP 1 Score: 877.9 bits (2267), Expect = 1.0e-251
Identity = 441/596 (73.99%), Postives = 498/596 (83.56%), Query Frame = 1

Query: 1   MDLSNSQSNISRG----RVSPPHRTQIADDSILLHLDSSRRGPTYSAPPVPLQLMEPQTE 60
           MDLS+  SN+S G      SPP R  I D+SI L L+SS R P++  PPVPLQL+EPQT 
Sbjct: 1   MDLSHPHSNLSLGFSSSHASPPRRPPIPDESITLQLESSLRDPSHPLPPVPLQLLEPQTA 60

Query: 61  ----NHRDENGDAKRDDDECDDRDVEEFRILGHSMCLKRRRD--GESSSLLGSTKRVSC- 120
               NH  E  DA  D+D   DRDVEEFRILGHSMCLKRRRD    SSS + S+KRVS  
Sbjct: 61  TDNGNHDSEFNDAADDED---DRDVEEFRILGHSMCLKRRRDCDSSSSSSISSSKRVSVS 120

Query: 121 -EFDMETRRSSVRSWGNQTLRAADPKLYGIMEKEKQRQINGIELIASENYVCRAVMEALG 180
            E D+E RR+ VRSWGNQ+L+ ADP ++ IMEKEK+RQ  GIELIASEN+VC+AVMEALG
Sbjct: 121 HESDLEARRALVRSWGNQSLQLADPDVFDIMEKEKRRQYRGIELIASENFVCKAVMEALG 180

Query: 181 SHLTNKYSEGMPGARYYGGNQYIDEIEILCRERALAAFDLNPHSWGVNVQPYSCTSANFA 240
           SHLTNKYSEGMPGARYYGGNQYIDEIE LC ERAL AF+L+P  WGVNVQPYSCTSANFA
Sbjct: 181 SHLTNKYSEGMPGARYYGGNQYIDEIETLCCERALLAFNLDPKCWGVNVQPYSCTSANFA 240

Query: 241 VYTGLLLPGDRIMALDSPSGGNPSHGYCTRNGKKVAGASIFFESFPYKVNPQTGYIDYDK 300
           VYTGLLLPGDRIM LD+PSGGN SHGY T NGKKV+GASIFFES PYKVNPQTGY+DY+K
Sbjct: 241 VYTGLLLPGDRIMGLDTPSGGNTSHGYYTPNGKKVSGASIFFESLPYKVNPQTGYVDYEK 300

Query: 301 LEERALDFRPKLLICGGSAYPRELDYARFRQIADKCGAVLMCDMAQISGLVAAKECVSPF 360
           LEERALDFRPK+LICGGS+YPRE DY+RFR IADKCGAVLMCDMAQISGL+AAKEC +PF
Sbjct: 301 LEERALDFRPKILICGGSSYPREWDYSRFRHIADKCGAVLMCDMAQISGLIAAKECSNPF 360

Query: 361 EYCDVVTSTTHKSLRGPRGGIIFYKKGYKPRGRAIVLYQGNDVKEYDFEERINFAVFPSL 420
           +YCD+VTSTTHKSLRGPRGGIIFY+KG KPR R I+L QGN+  +YDFEE+INFAVFPSL
Sbjct: 361 DYCDIVTSTTHKSLRGPRGGIIFYRKGAKPRKRGILLSQGNESDQYDFEEKINFAVFPSL 420

Query: 421 QGGPHNNHIAALAVALKQVATLEYKGYMQQVKKNAQALASALLRRNYKLVTGGTDNHMVL 480
           QGGPHNNHIAALA+ALKQVAT EYK YM QVKKNAQALASALLRR  +LVTGGTDNH+VL
Sbjct: 421 QGGPHNNHIAALAIALKQVATPEYKAYMHQVKKNAQALASALLRRKCRLVTGGTDNHLVL 480

Query: 481 WDLRSLGLSGKNLEQLCEMCHITLNKIIISGDNGVITTGGVRIGTPAMTSRGCLESDFEL 540
           WDLR LGL+GK  E++CE CHITLNKI I GDNGVI  GGVRIGTPAMTSRGCLE+DFE 
Sbjct: 481 WDLRPLGLTGKFYEKVCEACHITLNKIAIFGDNGVIIPGGVRIGTPAMTSRGCLEADFET 540

Query: 541 IVEFLHTAAQIAICIQREYGKMPNAFLTGLQSNKEVVELGNRVESFSAKFSMPGVE 585
           + EFL  AAQ+A  +QRE+GKM    L GL S+++VV+L  RVE+F+ +F+MPG +
Sbjct: 541 MAEFLFRAAQLANVLQREHGKMQKTVLKGLDSSRDVVDLRARVEAFATQFAMPGFD 593

BLAST of Csa3G221750 vs. NCBI nr
Match: gi|357445445|ref|XP_003593000.1| (serine hydroxymethyltransferase [Medicago truncatula])

HSP 1 Score: 867.1 bits (2239), Expect = 1.8e-248
Identity = 429/595 (72.10%), Postives = 498/595 (83.70%), Query Frame = 1

Query: 1   MDLSNSQSNIS----RGRVSPPHRTQIADDSILLHLDSSRRGPTYSAPPVPLQLMEPQTE 60
           MDLS+ QSN+S        SPP R  I DDSI LHL+SS R P+   P VPLQLMEPQTE
Sbjct: 1   MDLSHHQSNLSLNFSSSHASPPPRPPIPDDSIALHLESSFRDPSNPVPTVPLQLMEPQTE 60

Query: 61  NHRDENGDAKRDDDECDDRDVEEFRILGHSMCLKRRRDGESSSLLGSTKRVSCE------ 120
                  D + ++DE DDR+VEEFRILGHSMC+KRRRD ESS++  +TKR S E      
Sbjct: 61  KENGNAIDIESNNDE-DDREVEEFRILGHSMCIKRRRDSESSTI--TTKRASVESDRGDR 120

Query: 121 -FDMETRRSSVRSWGNQTLRAADPKLYGIMEKEKQRQINGIELIASENYVCRAVMEALGS 180
             D+E+R+++VRSWG+Q L+ ADP +Y I+EKEK+RQ  GIELIASEN+VCRAVMEALGS
Sbjct: 121 LLDLESRKAAVRSWGDQPLQVADPDIYEIIEKEKKRQFKGIELIASENFVCRAVMEALGS 180

Query: 181 HLTNKYSEGMPGARYYGGNQYIDEIEILCRERALAAFDLNPHSWGVNVQPYSCTSANFAV 240
           HLTNKYSEGMPGARYYGGNQYIDEIE LC ERALAAF+L+P  WGVNVQPYSCTSANFAV
Sbjct: 181 HLTNKYSEGMPGARYYGGNQYIDEIETLCCERALAAFNLDPKCWGVNVQPYSCTSANFAV 240

Query: 241 YTGLLLPGDRIMALDSPSGGNPSHGYCTRNGKKVAGASIFFESFPYKVNPQTGYIDYDKL 300
           YTGLL PGDRIM LD+PSGGN SHGY T NGKKV+GASIFFES  YK+NPQ+G+IDYDKL
Sbjct: 241 YTGLLAPGDRIMGLDTPSGGNTSHGYYTPNGKKVSGASIFFESLAYKINPQSGFIDYDKL 300

Query: 301 EERALDFRPKLLICGGSAYPRELDYARFRQIADKCGAVLMCDMAQISGLVAAKECVSPFE 360
           EERALDFRPK+LICGGS+YPRE DYARFR +ADKCGAVL+CDMAQISG++AAKECV+PF+
Sbjct: 301 EERALDFRPKILICGGSSYPREWDYARFRHVADKCGAVLLCDMAQISGIIAAKECVNPFD 360

Query: 361 YCDVVTSTTHKSLRGPRGGIIFYKKGYKPRGRAIVLYQGNDVKEYDFEERINFAVFPSLQ 420
           YCDVVTSTTHKSLRGPRGGIIFY+KG KPR R I+L QG++  +YDFEE+INFAVFPSLQ
Sbjct: 361 YCDVVTSTTHKSLRGPRGGIIFYRKGTKPRKRGILLTQGHESDQYDFEEKINFAVFPSLQ 420

Query: 421 GGPHNNHIAALAVALKQVATLEYKGYMQQVKKNAQALASALLRRNYKLVTGGTDNHMVLW 480
           GGPHNNHIAALA+ALKQVAT EYK YMQQVKKNAQALASALLRR  +LVTGGTDNH++LW
Sbjct: 421 GGPHNNHIAALAIALKQVATPEYKAYMQQVKKNAQALASALLRRKCRLVTGGTDNHLILW 480

Query: 481 DLRSLGLSGKNLEQLCEMCHITLNKIIISGDNGVITTGGVRIGTPAMTSRGCLESDFELI 540
           DLR LGL+GK  E++CE CHITLNKI I GDNG+I  GGVRIGTPAMTSRGCLE+DFE +
Sbjct: 481 DLRPLGLTGKFYEKVCEACHITLNKIAIFGDNGIIIPGGVRIGTPAMTSRGCLEADFETM 540

Query: 541 VEFLHTAAQIAICIQREYGKMPNAFLTGLQSNKEVVELGNRVESFSAKFSMPGVE 585
            +FL  AAQIA  +QRE+GK+       L+SN++++EL  RVE+F+ +F++PG +
Sbjct: 541 ADFLFRAAQIANMLQREHGKLQKTISKVLESNRDILELRARVEAFATQFALPGFD 592