Cp4.1LG00g00270 (gene) Cucurbita pepo (Zucchini)

Overview
NameCp4.1LG00g00270
Typegene
OrganismCucurbita pepo (Cucurbita pepo (Zucchini))
DescriptionPectinesterase
LocationCp4.1LG00 : 494870 .. 496688 (+)
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAATCCCATCTTCTGTTTCCTGTCATTATCATCATTCCATCTTCCTTCCTTCCAATCCAAAATATCTTACCAGACCTCGAATTCGCCGGAATTAATGGCTCCTCACATCCAAATCGCATTTTTCTCCATAGCCCTTTTCGCTCTTCTTTCTAATTCCTCTTCTTCACTCACAAAAACCAGTGCCAACACCATCAACAAATGGTGCAATACGACGCCGCATCCTAAGGTTTGCATTTTCTTCATGTCACCCCTCAAATCTCCACCCCAAAATCGACTCCAGTTTAGGAAATTGGCGATCAAAACCACGTTGGAAGGAGCCGCCAAGGCTCAAAATTACGTGTCCCGATTCGGTCCCACCTGTAAAACCACTCGCCAGAGAGCCGCTTGGACCGATTGCTTCAACCTCTATAACGATACCGTTTTTCAATTAAACCGTACTATCCAATGTCTCGACGCTGGTACTCGCCTCCGGCATTGTACGGATTTCGATGCGCAAACTTGGCTCAGTTCTGCTCTCACTAACATCGATATGTGCAGCTCCGGCGCGGCGGATCTCAATGTCACGGATTTTATTACTCCCATCAAATGTCAGAATGTTTCCAAGATGATTAGCAATTGTTTGGCCATTAATGGGAGATTCTTAGAGGAAGAGACTAAAAGCAGAACAACGGGAACAGAGGAATATCAAAATGGGTTTCCCACATGGCTTTCCACTGGTGATCGGAAATTGTTGCAGTCGGCGAGGGTTAGAGCTCATTTGGTGGTGGCGAAAGATGGGTCTGGCAAATTTAGGACGGTTCAGGCGGCAATCAACGCCGCCGCGAGAAGGCGTGGGAGAAGCCGCTTTGTAATTTACATAAAGCGTGGGGTTTATAAAGAAAACATTGAAGTTGGGAATGATAATGCGAATATAATGCTGGTCGGAGATGGGATTAGATTTACCGTGATTACAAGCGGAAGAAGCGTCGCTGCCGGCTTCACCACCTTCAATTCTGCAACCGCAGGTATGCGGTTAAATATGACATATATGGTAAATTATAAGTTAGTACTAAATATTTTTGACATTAGGTATCCAAGGTCCAGGGTTCATGGCTCGAGGCATAAGGTTTGTGAACACAGCCGGACCAAGAATGGGGCAAGCAGTGGCACTACGGTCATCATCGGACCTATCGGTGTTCCATCGGTGCAGCTTCGAGGGCTACCAAGACACACTCATGGTGCTGTCACAGAGACAATTCTACAAGCAATGCTACGTATACGGTACAATCGACTTCATTTTTGGGAACGCGGCAGTGGTTCTACAAAACTGCTTGATTTACGTGCGAAGGCCTTTAAAGGGGCAAGTGAACGTGATCACCGCGCAGGGCCGAGAGGACCCATTTCAGAACAGCGGAATCTCGATCCACAACTCGCAAATACGGGCGGCGGCTGATTTGCAGCCCATGATTGGGTCTGTGAAGACGTACTTGGGGCGGCCCTGGAAGAAGTACTCGCGGACCGTCATCATGAGAAGCTATATTGATAGCTTGGTGAGCCCAATGGGTTGGTTGGCGTGGCAAGGTACTAGATTTGCTCAAGATACGTTGTATTATGGGGAGTATCGTAACTTTGGGCCCAGAGCCTCCACCCGGCTTCGAGTCAGATGGCCTGGTTTTCATGCCATTACAACCCGAAATGTGGCCTCTAAGTTCACCGTCCGGAGACTCATCGCCGGCCATACTTGGCTTCCGGCCACTGGAGTTCCCTTCAAGCTTGACCTCTGA

mRNA sequence

ATGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAATCCCATCTTCTGTTTCCTGTCATTATCATCATTCCATCTTCCTTCCTTCCAATCCAAAATATCTTACCAGACCTCGAATTCGCCGGAATTAATGGCTCCTCACATCCAAATCGCATTTTTCTCCATAGCCCTTTTCGCTCTTCTTTCTAATTCCTCTTCTTCACTCACAAAAACCAGTGCCAACACCATCAACAAATGGTGCAATACGACGCCGCATCCTAAGGTTTGCATTTTCTTCATGTCACCCCTCAAATCTCCACCCCAAAATCGACTCCAGTTTAGGAAATTGGCGATCAAAACCACGTTGGAAGGAGCCGCCAAGGCTCAAAATTACGTGTCCCGATTCGGTCCCACCTGTAAAACCACTCGCCAGAGAGCCGCTTGGACCGATTGCTTCAACCTCTATAACGATACCGTTTTTCAATTAAACCGTACTATCCAATGTCTCGACGCTGGTACTCGCCTCCGGCATTGTACGGATTTCGATGCGCAAACTTGGCTCAGTTCTGCTCTCACTAACATCGATATGTGCAGCTCCGGCGCGGCGGATCTCAATGTCACGGATTTTATTACTCCCATCAAATGTCAGAATGTTTCCAAGATGATTAGCAATTGTTTGGCCATTAATGGGAGATTCTTAGAGGAAGAGACTAAAAGCAGAACAACGGGAACAGAGGAATATCAAAATGGGTTTCCCACATGGCTTTCCACTGGTGATCGGAAATTGTTGCAGTCGGCGAGGGTTAGAGCTCATTTGGTGGTGGCGAAAGATGGGTCTGGCAAATTTAGGACGGTTCAGGCGGCAATCAACGCCGCCGCGAGAAGGCGTGGGAGAAGCCGCTTTGTAATTTACATAAAGCGTGGGGTTTATAAAGAAAACATTGAAGTTGGGAATGATAATGCGAATATAATGCTGGTCGGAGATGGGATTAGATTTACCCCCATGATTGGGTCTGTGAAGACGTACTTGGGGCGGCCCTGGAAGAAGTACTCGCGGACCGTCATCATGAGAAGCTATATTGATAGCTTGGTGAGCCCAATGGGTTGGTTGGCGTGGCAAGGTACTAGATTTGCTCAAGATACGTTGTATTATGGGGAGTATCGTAACTTTGGGCCCAGAGCCTCCACCCGGCTTCGAGTCAGATGGCCTGGTTTTCATGCCATTACAACCCGAAATGTGGCCTCTAAGTTCACCGTCCGGAGACTCATCGCCGGCCATACTTGGCTTCCGGCCACTGGAGTTCCCTTCAAGCTTGACCTCTGA

Coding sequence (CDS)

ATGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAATCCCATCTTCTGTTTCCTGTCATTATCATCATTCCATCTTCCTTCCTTCCAATCCAAAATATCTTACCAGACCTCGAATTCGCCGGAATTAATGGCTCCTCACATCCAAATCGCATTTTTCTCCATAGCCCTTTTCGCTCTTCTTTCTAATTCCTCTTCTTCACTCACAAAAACCAGTGCCAACACCATCAACAAATGGTGCAATACGACGCCGCATCCTAAGGTTTGCATTTTCTTCATGTCACCCCTCAAATCTCCACCCCAAAATCGACTCCAGTTTAGGAAATTGGCGATCAAAACCACGTTGGAAGGAGCCGCCAAGGCTCAAAATTACGTGTCCCGATTCGGTCCCACCTGTAAAACCACTCGCCAGAGAGCCGCTTGGACCGATTGCTTCAACCTCTATAACGATACCGTTTTTCAATTAAACCGTACTATCCAATGTCTCGACGCTGGTACTCGCCTCCGGCATTGTACGGATTTCGATGCGCAAACTTGGCTCAGTTCTGCTCTCACTAACATCGATATGTGCAGCTCCGGCGCGGCGGATCTCAATGTCACGGATTTTATTACTCCCATCAAATGTCAGAATGTTTCCAAGATGATTAGCAATTGTTTGGCCATTAATGGGAGATTCTTAGAGGAAGAGACTAAAAGCAGAACAACGGGAACAGAGGAATATCAAAATGGGTTTCCCACATGGCTTTCCACTGGTGATCGGAAATTGTTGCAGTCGGCGAGGGTTAGAGCTCATTTGGTGGTGGCGAAAGATGGGTCTGGCAAATTTAGGACGGTTCAGGCGGCAATCAACGCCGCCGCGAGAAGGCGTGGGAGAAGCCGCTTTGTAATTTACATAAAGCGTGGGGTTTATAAAGAAAACATTGAAGTTGGGAATGATAATGCGAATATAATGCTGGTCGGAGATGGGATTAGATTTACCCCCATGATTGGGTCTGTGAAGACGTACTTGGGGCGGCCCTGGAAGAAGTACTCGCGGACCGTCATCATGAGAAGCTATATTGATAGCTTGGTGAGCCCAATGGGTTGGTTGGCGTGGCAAGGTACTAGATTTGCTCAAGATACGTTGTATTATGGGGAGTATCGTAACTTTGGGCCCAGAGCCTCCACCCGGCTTCGAGTCAGATGGCCTGGTTTTCATGCCATTACAACCCGAAATGTGGCCTCTAAGTTCACCGTCCGGAGACTCATCGCCGGCCATACTTGGCTTCCGGCCACTGGAGTTCCCTTCAAGCTTGACCTCTGA

Protein sequence

MKKKKKKKKKKKKKKKKKKNPIFCFLSLSSFHLPSFQSKISYQTSNSPELMAPHIQIAFFSIALFALLSNSSSSLTKTSANTINKWCNTTPHPKVCIFFMSPLKSPPQNRLQFRKLAIKTTLEGAAKAQNYVSRFGPTCKTTRQRAAWTDCFNLYNDTVFQLNRTIQCLDAGTRLRHCTDFDAQTWLSSALTNIDMCSSGAADLNVTDFITPIKCQNVSKMISNCLAINGRFLEEETKSRTTGTEEYQNGFPTWLSTGDRKLLQSARVRAHLVVAKDGSGKFRTVQAAINAAARRRGRSRFVIYIKRGVYKENIEVGNDNANIMLVGDGIRFTPMIGSVKTYLGRPWKKYSRTVIMRSYIDSLVSPMGWLAWQGTRFAQDTLYYGEYRNFGPRASTRLRVRWPGFHAITTRNVASKFTVRRLIAGHTWLPATGVPFKLDL
Homology
[Close]
BLAST of Cp4.1LG00g00270 vs. Swiss-Prot
Match: PME33_ARATH (Probable pectinesterase/pectinesterase inhibitor 33 OS=Arabidopsis thaliana GN=PME33 PE=2 SV=1)

HSP 1 Score: 249.6 bits (636), Expect = 6.3e-65
Identity = 129/259 (49.81%), Postives = 178/259 (68.73%), Query Frame = 1 

Query: 86  WCNTTPHPKVCIFFMSPLKS------PPQNRLQFRKLAIKTTLEGAAKAQNYVSRFGPTC 145
           WCN TPH + C ++            PP+ R +F ++ +K  L+ A    +   +FGP+C
Sbjct: 87  WCNKTPHAETCNYYFRKSSQNNINLRPPRFRSEFLRMLVKVALDQAVITHSQTVKFGPSC 146

Query: 146 KTTRQRAAWTDCFNLYNDTVFQLNRTIQCLD-AGTRLRHCTDFDAQTWLSSALTNIDMCS 205
              +++AAW+DC NL+ +TV QLNRT++ L+ A +    CTDFDAQTWLS+A TNI+ C 
Sbjct: 147 TNNQRKAAWSDCVNLFQNTVAQLNRTLKGLNPAASSDVKCTDFDAQTWLSTAQTNIETCR 206

Query: 206 SGAADLNVTDFITP-IKCQNVSKMISNCLAINGRFLEEETKSRTTGTEEYQNGFPTWLST 265
           SG+ DLNV+DF+ P I  +N+S +I NCLA+NG  +++   + T   +EY   FP+W+S 
Sbjct: 207 SGSEDLNVSDFVMPVISNKNLSDLIGNCLAVNGVLMKQHDHTTTANHKEY---FPSWVSR 266

Query: 266 GDRKLLQSARVRA---HLVVAKDGSGKFRTVQAAINAAARRRGRSRFVIYIKRGVYKENI 325
            +R+LL SA +     HLVVA+D SG FR++QAAIN AARRR +SRFVIY+K+GVY+ENI
Sbjct: 267 HERRLLVSASLAKSSPHLVVAQDRSGHFRSIQAAINFAARRRFKSRFVIYVKKGVYRENI 326

Query: 326 EVGNDNANIMLVGDGIRFT 334
           +VGNDN NIMLVGDG R T
Sbjct: 327 DVGNDNHNIMLVGDGERKT 342

[Close]
BLAST of Cp4.1LG00g00270 vs. Swiss-Prot
Match: PME60_ARATH (Probable pectinesterase/pectinesterase inhibitor 60 OS=Arabidopsis thaliana GN=PME60 PE=2 SV=1)

HSP 1 Score: 235.3 bits (599), Expect = 1.2e-60
Identity = 136/289 (47.06%), Postives = 184/289 (63.67%), Query Frame = 1 

Query: 57  IAFFSIALFALLSNSSSSLTKTS---ANTINKWCNTTPHPKVCIFFM---SPLKSPPQNR 116
           I+F S+ L  +L      LT  +   +  I+ WC+ TP+P  C  +    S  + P Q  
Sbjct: 9   ISFLSLHLLLILLLCLRPLTTVADGNSTNIDGWCDKTPYPYPCKRYFIKHSGFRLPTQIS 68

Query: 117 LQFRKLAIKTTLEGAAKAQNYVSRFGPTCKTTRQRAAWTDCFNLYNDTVFQLNRTIQCLD 176
            +FR L ++  ++ A  A + ++     C   +++A   DC NLY DTV QLNRT+Q + 
Sbjct: 69  -EFRVLLVEAAMDRAVSAWDKLTNSSKNCTDFKKQAVLADCINLYGDTVMQLNRTLQGVS 128

Query: 177 AGTRLRHCTDFDAQTWLSSALTNIDMCSSGAADLNVTDFITPIKCQN-VSKMISNCLAIN 236
           + T  R CTDFDAQTWLS+ALTN + C  G++DLNV+DF TPI     +S +ISNCLA+N
Sbjct: 129 SKTG-RRCTDFDAQTWLSTALTNTETCRRGSSDLNVSDFTTPIVSNTKISHLISNCLAVN 188

Query: 237 GRFLEEETKSRTTGTEEYQNGFPTWLSTGDRKLLQSARVRAHLVVAKDGSGKFRTVQAAI 296
           G  L       TTG  +   GFPTW+S  +R+LLQ   VRA+LVVAKDGSG F+TVQAAI
Sbjct: 189 GALLTAGKNDSTTGDSK---GFPTWVSRKERRLLQLQSVRANLVVAKDGSGHFKTVQAAI 248

Query: 297 NAAARRRGRS-RFVIYIKRGVYKENIEVGNDNANIMLVGDGIRFTPMIG 338
           + A RR+  S RFVIY+KRG+Y+EN+ V  +N NIMLVGDG+R+T + G
Sbjct: 249 DVAGRRKVTSGRFVIYVKRGIYQENLNVRLNNDNIMLVGDGMRYTIITG 292

[Close]
BLAST of Cp4.1LG00g00270 vs. Swiss-Prot
Match: PME59_ARATH (Probable pectinesterase/pectinesterase inhibitor 59 OS=Arabidopsis thaliana GN=PME59 PE=2 SV=1)

HSP 1 Score: 229.2 bits (583), Expect = 8.9e-59
Identity = 134/294 (45.58%), Postives = 180/294 (61.22%), Query Frame = 1 

Query: 50  LMAPHIQIAFFSIALFALLSNSSSSLTKTSANTINKWCNTTPHPKVCIFFMSPLKS--PP 109
           +M   + I F  + L  LL      LT  +      WC+ TP+P  C  +         P
Sbjct: 3   MMMQKLSILFLHLILLVLLC--VHPLTTVADRNSTDWCDKTPYPDPCKCYFKNHNGFQQP 62

Query: 110 QNRLQFRKLAIKTTLEGAAKAQNYVSRFGPTCKTTRQRAAWTDCFNLYNDTVFQLNRTIQ 169
               +FR + ++  ++ A  A+  ++  G  C  ++++A   DC +LY DT+ QLNRT+ 
Sbjct: 63  TQLSEFRVMLVEAAMDRAISARAELTNSGKNCTDSKKQAVLADCIDLYGDTIMQLNRTLH 122

Query: 170 CLD--AGTRLRHCTDFDAQTWLSSALTNIDMCSSGAADLNVTDFITPIKCQ-NVSKMISN 229
            +   AG   + CTDFDAQTWLS+ALTN + C  G++DLNVTDFITPI     +S +ISN
Sbjct: 123 GVSPKAGAA-KSCTDFDAQTWLSTALTNTETCRRGSSDLNVTDFITPIVSNTKISHLISN 182

Query: 230 CLAINGRFLEEETKSRTTGTEEYQNGFPTWLSTGDRKLLQSARVRAHLVVAKDGSGKFRT 289
           CLA+NG  L    K  TT     Q GFPTWLS  D++LL++  VRA+LVVAKDGSG F T
Sbjct: 183 CLAVNGALLTAGNKGNTTAN---QKGFPTWLSRKDKRLLRA--VRANLVVAKDGSGHFNT 242

Query: 290 VQAAINAAARRRGRS-RFVIYIKRGVYKENIEVGNDNANIMLVGDGIRFTPMIG 338
           VQAAI+ A RR+  S RFVIY+KRG+Y+ENI V  +N +IMLVGDG+R T + G
Sbjct: 243 VQAAIDVAGRRKVTSGRFVIYVKRGIYQENINVRLNNDDIMLVGDGMRSTIITG 288

[Close]
BLAST of Cp4.1LG00g00270 vs. Swiss-Prot
Match: PME17_ARATH (Probable pectinesterase/pectinesterase inhibitor 17 OS=Arabidopsis thaliana GN=PME17 PE=2 SV=2)

HSP 1 Score: 206.1 bits (523), Expect = 8.0e-52
Identity = 112/269 (41.64%), Postives = 168/269 (62.45%), Query Frame = 1 

Query: 72  SSSLTKTSANTINKWCNTTPHPKVCIFFMSPLKS--PPQNRLQFRKLAIKTTLEGAAKAQ 131
           +S+++  +   +  WC+ TP+PK C +F++   +  P ++  +F K+++K  L+ A  A+
Sbjct: 19  ASTVSGYNQKDVKAWCSQTPNPKPCEYFLTHNSNNEPIKSESEFLKISMKLVLDRAILAK 78

Query: 132 NYVSRFGPTCKTTRQRAAWTDCFNLYNDTVFQLNRTIQCLDAGTRLRHCTDFDAQTWLSS 191
            +    GP C+ TR++AAW DC  LY+ TV ++N T   +D   +   C+  DAQTWLS+
Sbjct: 79  THAFTLGPKCRDTREKAAWEDCIKLYDLTVSKINET---MDPNVK---CSKLDAQTWLST 138

Query: 192 ALTNIDMCSSGAADLNVTDFITPIKCQNVSKMISNCLAINGRFLEEETKSRTTGTEEYQN 251
           ALTN+D C +G  +L VTD + P+   NVS ++ N LAIN        K     T   ++
Sbjct: 139 ALTNLDTCRAGFLELGVTDIVLPLMSNNVSNLLCNTLAIN--------KVPFNYTPPEKD 198

Query: 252 GFPTWLSTGDRKLLQSARVRAHLVVAKDGSGKFRTVQAAINAAARRRGRSRFVIYIKRGV 311
           GFP+W+  GDRKLLQS+  + + VVAKDGSG F+T++ AI+AA+   G  RFVIY+K+GV
Sbjct: 199 GFPSWVKPGDRKLLQSSTPKDNAVVAKDGSGNFKTIKEAIDAAS---GSGRFVIYVKQGV 258

Query: 312 YKENIEVGNDNANIMLVGDGIRFTPMIGS 339
           Y EN+E+     N+ML GDGI  T + GS
Sbjct: 259 YSENLEI--RKKNVMLRGDGIGKTIITGS 268

[Close]
BLAST of Cp4.1LG00g00270 vs. Swiss-Prot
Match: PME2_CITSI (Pectinesterase 2 OS=Citrus sinensis GN=PECS-2.1 PE=2 SV=1)

HSP 1 Score: 191.8 bits (486), Expect = 1.6e-47
Identity = 116/280 (41.43%), Postives = 160/280 (57.14%), Query Frame = 1 

Query: 61  SIALFALLSNSSSSLTKTSANTINKWCNTTPHPKVCIFFMSPLKSPPQNR--LQFRKLAI 120
           S+ LF+L   S       S   +  WC  TP+P+ C +F++        +    F K+++
Sbjct: 10  SLVLFSLSHTSFGY----SPEEVKSWCGKTPNPQPCEYFLTQKTDVTSIKQDTDFYKISL 69

Query: 121 KTTLEGAAKAQNYVSRFGPTCKTTRQRAAWTDCFNLYNDTVFQLNRTIQCLDAGTRLRHC 180
           +  LE A  AQ+     G  C+  R++AAW DC  LY  TV +LN+T            C
Sbjct: 70  QLALERATTAQSRTYTLGSKCRNEREKAAWEDCRELYELTVLKLNQTSNSSPG------C 129

Query: 181 TDFDAQTWLSSALTNIDMCSSGAADLNVTDFITPIKCQNVSKMISNCLAINGRFLEEETK 240
           T  D QTWLS+ALTN++ C +   DL V +++ P+   NV+K+ISN L++N     E + 
Sbjct: 130 TKVDKQTWLSTALTNLETCRASLEDLGVPEYVLPLLSNNVTKLISNTLSLNKVPYNEPS- 189

Query: 241 SRTTGTEEYQNGFPTWLSTGDRKLLQSARVRAHLVVAKDGSGKFRTVQAAINAAARRRGR 300
                   Y++GFPTW+  GDRKLLQ+   RA++VVA+DGSG  +T+Q A+ AAA R G 
Sbjct: 190 --------YKDGFPTWVKPGDRKLLQTT-PRANIVVAQDGSGNVKTIQEAV-AAASRAGG 249

Query: 301 SRFVIYIKRGVYKENIEVGNDNANIMLVGDGIRFTPMIGS 339
           SR+VIYIK G Y ENIEV     NIM VGDGI  T + GS
Sbjct: 250 SRYVIYIKAGTYNENIEV--KLKNIMFVGDGIGKTIITGS 266

[Close]
BLAST of Cp4.1LG00g00270 vs. TrEMBL
Match: A0A0A0L330_CUCSA (Pectinesterase OS=Cucumis sativus GN=Csa_3G088980 PE=4 SV=1)

HSP 1 Score: 391.3 bits (1004), Expect = 1.5e-105
Identity = 202/288 (70.14%), Postives = 229/288 (79.51%), Query Frame = 1 

Query: 51  MAPHIQIAFFSIALFALLSNSSSSLTKTSANTINKWCNTTPHPKVCIFFMSPLKSPPQNR 110
           MA H +I F SI  F LLSN+       SAN I KWC TTPHP+VC+  MSP+K PPQNR
Sbjct: 1   MASHYKILFISITFFTLLSNN------VSANPIIKWCKTTPHPRVCVSLMSPIKPPPQNR 60

Query: 111 LQFRKLAIKTTLEGAAKAQNYVSRFGPTCKTTRQRAAWTDCFNLYNDTVFQLNRTIQCL- 170
            +FR++AI+TTLE AA+A+ Y +RFGPTCKT+RQR AWTDCF LYND V QLNRT+ C+ 
Sbjct: 61  REFRQMAIQTTLEKAAEARAYTARFGPTCKTSRQRTAWTDCFKLYNDVVLQLNRTLHCVV 120

Query: 171 -DAGTRLRHCTDFDAQTWLSSALTNIDMCSSGAADLNVTDFITPIKCQNVSKMISNCLAI 230
            D     R CTDFDAQTWLSSALT+ID+C+SGAADLNVTDFITPIKC NVSKMISNCLAI
Sbjct: 121 TDEAIHRRSCTDFDAQTWLSSALTDIDLCNSGAADLNVTDFITPIKCLNVSKMISNCLAI 180

Query: 231 NGRFLEEETKSRTTGTEEYQNG-FPTWLSTGDRKLLQS--ARVRAHLVVAKDGSGKFRTV 290
           NG FLEEE      G    +NG FP W+S GDRKLL+S   RVRA+LVVAKDGSG FR V
Sbjct: 181 NGGFLEEEGVKYDDG----RNGSFPMWVSEGDRKLLESRPGRVRANLVVAKDGSGTFRRV 240

Query: 291 QAAINAAARRRGRSRFVIYIKRGVYKENIEVGNDNANIMLVGDGIRFT 334
           QAAI+AAARRRGR RF+IY+KRGVY+ENIEVGNDN NIMLVGDG+RFT
Sbjct: 241 QAAIDAAARRRGRGRFIIYVKRGVYRENIEVGNDNGNIMLVGDGMRFT 278

[Close]
BLAST of Cp4.1LG00g00270 vs. TrEMBL
Match: V4SD84_9ROSI (Pectinesterase OS=Citrus clementina GN=CICLE_v10004719mg PE=4 SV=1)

HSP 1 Score: 266.9 bits (681), Expect = 4.3e-68
Identity = 144/284 (50.70%), Postives = 195/284 (68.66%), Query Frame = 1 

Query: 54  HIQIAFFSIALFALLSNSSSSLTKTSANTINKWCNTTPHPKVCIFFMSPL--KSPPQNRL 113
           +I +    I+L ++  ++ S   + SA  I  WC+ TPHP+ C +F+S    +   ++R 
Sbjct: 5   NISLLIIFISLSSIFYHALSR--RPSATNITWWCSKTPHPEPCNYFLSRSHHRYTFKHRS 64

Query: 114 QFRKLAIKTTLEGAAKAQNYVSRFGPTCKTTRQRAAWTDCFNLYNDTVFQLNRTIQCLDA 173
            FRK+ ++  +E A KA + V  FG  C+   Q+AAW+DC  LY +TV QLNRT+Q L +
Sbjct: 65  DFRKIIVQVAIEQAHKAHDQVMEFGTNCENKHQKAAWSDCVKLYANTVLQLNRTLQGLKS 124

Query: 174 GTRLRHCTDFDAQTWLSSALTNIDMCSSGAADLNVTDFITPIKCQNVSKMISNCLAINGR 233
               R CTDFDAQTWLS+ALTNI  C SG  +LNV+DFITP+   N+S++ISN LA+NG 
Sbjct: 125 D---RSCTDFDAQTWLSTALTNIRTCQSGYMELNVSDFITPVMSNNLSQLISNSLAVNGV 184

Query: 234 FLEEETKSRTTGTEEYQNGFPTWLSTGDRKLLQSARV--RAHLVVAKDGSGKFRTVQAAI 293
            L+ E  +       Y NGFP+WLS  +R LL+S+ +  RA+LVVA DGSG +RT+QAAI
Sbjct: 185 LLKRENVT-------YTNGFPSWLSGHERNLLESSSLEARANLVVATDGSGNYRTIQAAI 244

Query: 294 NAAARRRGRSRFVIYIKRGVYKENIEVGNDNANIMLVGDGIRFT 334
           NAAARRRG  RF+I++KRGVY+ENIEVG +N NIMLVG+G+R T
Sbjct: 245 NAAARRRGSGRFIIHVKRGVYRENIEVGLNNNNIMLVGEGMRNT 276

[Close]
BLAST of Cp4.1LG00g00270 vs. TrEMBL
Match: A0A067FDF5_CITSI (Pectinesterase (Fragment) OS=Citrus sinensis GN=CISIN_1g0366962mg PE=4 SV=1)

HSP 1 Score: 264.6 bits (675), Expect = 2.1e-67
Identity = 138/261 (52.87%), Postives = 182/261 (69.73%), Query Frame = 1 

Query: 77  KTSANTINKWCNTTPHPKVCIFFMSPL--KSPPQNRLQFRKLAIKTTLEGAAKAQNYVSR 136
           + SA  I  WC+ TPHP+ C +F+S    +   ++R  FRK+ ++  +E A KA   +  
Sbjct: 26  RPSATNITWWCSKTPHPEPCKYFLSRSHHRYTFKHRSDFRKIIVQVAIEQAHKAHEQLME 85

Query: 137 FGPTCKTTRQRAAWTDCFNLYNDTVFQLNRTIQCLDAGTRLRHCTDFDAQTWLSSALTNI 196
           FG  C+   Q+AAW+DC  LY +TV QLNRT+Q L +    R CTDFDAQTWLS+ALTNI
Sbjct: 86  FGTNCENKHQKAAWSDCVKLYANTVLQLNRTLQGLKSD---RSCTDFDAQTWLSTALTNI 145

Query: 197 DMCSSGAADLNVTDFITPIKCQNVSKMISNCLAINGRFLEEETKSRTTGTEEYQNGFPTW 256
             C SG  +LNV+DFITP+   N+S++ISN LA+NG  L+ E  +       Y NGFP+W
Sbjct: 146 RTCQSGYMELNVSDFITPVMSNNLSQLISNSLAVNGVLLKSENVT-------YTNGFPSW 205

Query: 257 LSTGDRKLLQSARV--RAHLVVAKDGSGKFRTVQAAINAAARRRGRSRFVIYIKRGVYKE 316
           LS  +R LL+S+ +  RA+LVVA DGSG +RT+QAAINAAA RRG  RF+I++KRGVY+E
Sbjct: 206 LSGHERNLLESSSLEARANLVVATDGSGNYRTIQAAINAAAGRRGSGRFIIHVKRGVYRE 265

Query: 317 NIEVGNDNANIMLVGDGIRFT 334
           NIEVG +N NIMLVG+G+R T
Sbjct: 266 NIEVGLNNNNIMLVGEGMRNT 276

[Close]
BLAST of Cp4.1LG00g00270 vs. TrEMBL
Match: W9SDY8_9ROSA (Pectinesterase OS=Morus notabilis GN=L484_001126 PE=4 SV=1)

HSP 1 Score: 264.6 bits (675), Expect = 2.1e-67
Identity = 132/256 (51.56%), Postives = 186/256 (72.66%), Query Frame = 1 

Query: 81  NTINKWCNTTPHPKVCIFFMSP-LKSPPQNRLQFRKLAIKTTLEGAAKAQNYVSRFGPTC 140
           +TI++WC+ TPHP  C +++S    + P+ RL+FRK+ I+  +E A +A+ + S+   +C
Sbjct: 45  HTISRWCSQTPHPGPCNYYISRRFAAAPRRRLEFRKMLIQVAMERALEARGHASQLESSC 104

Query: 141 KTT-RQRAAWTDCFNLYNDTVFQLNRTIQCLDAGTRLRHCTDFDAQTWLSSALTNIDMCS 200
           +    ++AAW DC  LY+ TV QLNRT+  L  G   R CTDFDAQTWLS+ALTNID C 
Sbjct: 105 ENKPHEKAAWRDCLKLYDGTVLQLNRTLSGL-GGPHKRSCTDFDAQTWLSTALTNIDTCQ 164

Query: 201 SGAADLNVTDFITPIKC-QNVSKMISNCLAINGRFLEEETKSRTTGTEEYQNGFPTWLST 260
           +G+++LNV++FITP+ C  NVS+++ N LA+N + LE +       T+ +   FP+W+S 
Sbjct: 165 TGSSELNVSEFITPVACGYNVSELLCNSLAVNAKLLENQEAINYNETD-HDRDFPSWVSN 224

Query: 261 GDRKLLQSARVRAHLVVAKDGSGKFRTVQAAINAAARRRGRSRFVIYIKRGVYKENIEVG 320
            +R++L+S  ++A+LVVAKDGSG FR+VQ AIN AARRR  SRFVI++KRGVY+ENIEVG
Sbjct: 225 RERRMLESPGIKANLVVAKDGSGHFRSVQEAINRAARRRIHSRFVIHVKRGVYRENIEVG 284

Query: 321 NDNANIMLVGDGIRFT 334
             N NIMLVGDG+R+T
Sbjct: 285 VGNRNIMLVGDGLRYT 298

[Close]
BLAST of Cp4.1LG00g00270 vs. TrEMBL
Match: A0A061FZE1_THECC (Pectinesterase OS=Theobroma cacao GN=TCM_014943 PE=4 SV=1)

HSP 1 Score: 260.4 bits (664), Expect = 4.0e-66
Identity = 139/293 (47.44%), Postives = 196/293 (66.89%), Query Frame = 1 

Query: 55  IQIAFFSIALFALLSNSSSSLTKTSANTINKWCNTTPHPKVCIFFM--SPLKSPPQNRLQ 114
           +++    I L +  S  S++L + S + I  WC+ TPHP+ C +FM     +  P++  +
Sbjct: 1   MEVKILLIFLISFSSIFSTALARGSRSNITWWCSKTPHPEPCKYFMRHGSHRFAPEHMTE 60

Query: 115 FRKLAIKTTLEGAAKAQNYVSRFGPTCKTTRQRAAWTDCFNLYNDTVFQLNRTIQCLDAG 174
           FRK+ ++  L+ A  A+  V  FG +C++  Q+AAW DC  L+++T+ QLN T+Q L+ G
Sbjct: 61  FRKMIVRVALDRAVVAEKNVLEFGQSCESRHQKAAWRDCLKLFDNTILQLNLTLQGLETG 120

Query: 175 TRLRHCTDFDAQTWLSSALTNIDMCSSGAADLNVTDFITPIKCQNVSKMISNCLAINGRF 234
            R+  C+DFDAQTWLS+ALTNI  C +G  D NV+DF TPI   N+S++ISN LA+NG F
Sbjct: 121 RRIS-CSDFDAQTWLSTALTNIQTCEAGFMDFNVSDFSTPITSNNISQLISNSLAVNGVF 180

Query: 235 LEEETKSRTTGTEEYQNGFPTWLSTGDRKLLQSA--RVRAHLVVAKDGSGKFRTVQAAIN 294
           L  +  +  +         P WLS  DR LL SA  +V+AHLVVAKDGSG F+TVQAA++
Sbjct: 181 LHMKNHTEVS---------PGWLSRHDRWLLASASTKVKAHLVVAKDGSGNFKTVQAALD 240

Query: 295 AAARRRGRSRFVIYIKRGVYKENIEVGNDNANIMLVGDGIRFTPMIG--SVKT 342
           AAA+RR  SR +IY+K+GVY+ENIEV N N+NIML+GDG++ T +    SVKT
Sbjct: 241 AAAKRRRTSRLIIYVKKGVYRENIEVVNTNSNIMLIGDGMKRTIITSSRSVKT 283

[Close]
BLAST of Cp4.1LG00g00270 vs. TAIR10
Match: AT3G47400.1 (AT3G47400.1 Plant invertase/pectin methylesterase inhibitor superfamily)

HSP 1 Score: 249.6 bits (636), Expect = 3.6e-66
Identity = 129/259 (49.81%), Postives = 178/259 (68.73%), Query Frame = 1 

Query: 86  WCNTTPHPKVCIFFMSPLKS------PPQNRLQFRKLAIKTTLEGAAKAQNYVSRFGPTC 145
           WCN TPH + C ++            PP+ R +F ++ +K  L+ A    +   +FGP+C
Sbjct: 87  WCNKTPHAETCNYYFRKSSQNNINLRPPRFRSEFLRMLVKVALDQAVITHSQTVKFGPSC 146

Query: 146 KTTRQRAAWTDCFNLYNDTVFQLNRTIQCLD-AGTRLRHCTDFDAQTWLSSALTNIDMCS 205
              +++AAW+DC NL+ +TV QLNRT++ L+ A +    CTDFDAQTWLS+A TNI+ C 
Sbjct: 147 TNNQRKAAWSDCVNLFQNTVAQLNRTLKGLNPAASSDVKCTDFDAQTWLSTAQTNIETCR 206

Query: 206 SGAADLNVTDFITP-IKCQNVSKMISNCLAINGRFLEEETKSRTTGTEEYQNGFPTWLST 265
           SG+ DLNV+DF+ P I  +N+S +I NCLA+NG  +++   + T   +EY   FP+W+S 
Sbjct: 207 SGSEDLNVSDFVMPVISNKNLSDLIGNCLAVNGVLMKQHDHTTTANHKEY---FPSWVSR 266

Query: 266 GDRKLLQSARVRA---HLVVAKDGSGKFRTVQAAINAAARRRGRSRFVIYIKRGVYKENI 325
            +R+LL SA +     HLVVA+D SG FR++QAAIN AARRR +SRFVIY+K+GVY+ENI
Sbjct: 267 HERRLLVSASLAKSSPHLVVAQDRSGHFRSIQAAINFAARRRFKSRFVIYVKKGVYRENI 326

Query: 326 EVGNDNANIMLVGDGIRFT 334
           +VGNDN NIMLVGDG R T
Sbjct: 327 DVGNDNHNIMLVGDGERKT 342

[Close]
BLAST of Cp4.1LG00g00270 vs. TAIR10
Match: AT5G51500.1 (AT5G51500.1 Plant invertase/pectin methylesterase inhibitor superfamily)

HSP 1 Score: 235.3 bits (599), Expect = 7.0e-62
Identity = 136/289 (47.06%), Postives = 184/289 (63.67%), Query Frame = 1 

Query: 57  IAFFSIALFALLSNSSSSLTKTS---ANTINKWCNTTPHPKVCIFFM---SPLKSPPQNR 116
           I+F S+ L  +L      LT  +   +  I+ WC+ TP+P  C  +    S  + P Q  
Sbjct: 9   ISFLSLHLLLILLLCLRPLTTVADGNSTNIDGWCDKTPYPYPCKRYFIKHSGFRLPTQIS 68

Query: 117 LQFRKLAIKTTLEGAAKAQNYVSRFGPTCKTTRQRAAWTDCFNLYNDTVFQLNRTIQCLD 176
            +FR L ++  ++ A  A + ++     C   +++A   DC NLY DTV QLNRT+Q + 
Sbjct: 69  -EFRVLLVEAAMDRAVSAWDKLTNSSKNCTDFKKQAVLADCINLYGDTVMQLNRTLQGVS 128

Query: 177 AGTRLRHCTDFDAQTWLSSALTNIDMCSSGAADLNVTDFITPIKCQN-VSKMISNCLAIN 236
           + T  R CTDFDAQTWLS+ALTN + C  G++DLNV+DF TPI     +S +ISNCLA+N
Sbjct: 129 SKTG-RRCTDFDAQTWLSTALTNTETCRRGSSDLNVSDFTTPIVSNTKISHLISNCLAVN 188

Query: 237 GRFLEEETKSRTTGTEEYQNGFPTWLSTGDRKLLQSARVRAHLVVAKDGSGKFRTVQAAI 296
           G  L       TTG  +   GFPTW+S  +R+LLQ   VRA+LVVAKDGSG F+TVQAAI
Sbjct: 189 GALLTAGKNDSTTGDSK---GFPTWVSRKERRLLQLQSVRANLVVAKDGSGHFKTVQAAI 248

Query: 297 NAAARRRGRS-RFVIYIKRGVYKENIEVGNDNANIMLVGDGIRFTPMIG 338
           + A RR+  S RFVIY+KRG+Y+EN+ V  +N NIMLVGDG+R+T + G
Sbjct: 249 DVAGRRKVTSGRFVIYVKRGIYQENLNVRLNNDNIMLVGDGMRYTIITG 292

[Close]
BLAST of Cp4.1LG00g00270 vs. TAIR10
Match: AT5G51490.1 (AT5G51490.1 Plant invertase/pectin methylesterase inhibitor superfamily)

HSP 1 Score: 229.2 bits (583), Expect = 5.0e-60
Identity = 134/294 (45.58%), Postives = 180/294 (61.22%), Query Frame = 1 

Query: 50  LMAPHIQIAFFSIALFALLSNSSSSLTKTSANTINKWCNTTPHPKVCIFFMSPLKS--PP 109
           +M   + I F  + L  LL      LT  +      WC+ TP+P  C  +         P
Sbjct: 3   MMMQKLSILFLHLILLVLLC--VHPLTTVADRNSTDWCDKTPYPDPCKCYFKNHNGFQQP 62

Query: 110 QNRLQFRKLAIKTTLEGAAKAQNYVSRFGPTCKTTRQRAAWTDCFNLYNDTVFQLNRTIQ 169
               +FR + ++  ++ A  A+  ++  G  C  ++++A   DC +LY DT+ QLNRT+ 
Sbjct: 63  TQLSEFRVMLVEAAMDRAISARAELTNSGKNCTDSKKQAVLADCIDLYGDTIMQLNRTLH 122

Query: 170 CLD--AGTRLRHCTDFDAQTWLSSALTNIDMCSSGAADLNVTDFITPIKCQ-NVSKMISN 229
            +   AG   + CTDFDAQTWLS+ALTN + C  G++DLNVTDFITPI     +S +ISN
Sbjct: 123 GVSPKAGAA-KSCTDFDAQTWLSTALTNTETCRRGSSDLNVTDFITPIVSNTKISHLISN 182

Query: 230 CLAINGRFLEEETKSRTTGTEEYQNGFPTWLSTGDRKLLQSARVRAHLVVAKDGSGKFRT 289
           CLA+NG  L    K  TT     Q GFPTWLS  D++LL++  VRA+LVVAKDGSG F T
Sbjct: 183 CLAVNGALLTAGNKGNTTAN---QKGFPTWLSRKDKRLLRA--VRANLVVAKDGSGHFNT 242

Query: 290 VQAAINAAARRRGRS-RFVIYIKRGVYKENIEVGNDNANIMLVGDGIRFTPMIG 338
           VQAAI+ A RR+  S RFVIY+KRG+Y+ENI V  +N +IMLVGDG+R T + G
Sbjct: 243 VQAAIDVAGRRKVTSGRFVIYVKRGIYQENINVRLNNDDIMLVGDGMRSTIITG 288

[Close]
BLAST of Cp4.1LG00g00270 vs. TAIR10
Match: AT2G45220.1 (AT2G45220.1 Plant invertase/pectin methylesterase inhibitor superfamily)

HSP 1 Score: 206.1 bits (523), Expect = 4.5e-53
Identity = 112/269 (41.64%), Postives = 168/269 (62.45%), Query Frame = 1 

Query: 72  SSSLTKTSANTINKWCNTTPHPKVCIFFMSPLKS--PPQNRLQFRKLAIKTTLEGAAKAQ 131
           +S+++  +   +  WC+ TP+PK C +F++   +  P ++  +F K+++K  L+ A  A+
Sbjct: 19  ASTVSGYNQKDVKAWCSQTPNPKPCEYFLTHNSNNEPIKSESEFLKISMKLVLDRAILAK 78

Query: 132 NYVSRFGPTCKTTRQRAAWTDCFNLYNDTVFQLNRTIQCLDAGTRLRHCTDFDAQTWLSS 191
            +    GP C+ TR++AAW DC  LY+ TV ++N T   +D   +   C+  DAQTWLS+
Sbjct: 79  THAFTLGPKCRDTREKAAWEDCIKLYDLTVSKINET---MDPNVK---CSKLDAQTWLST 138

Query: 192 ALTNIDMCSSGAADLNVTDFITPIKCQNVSKMISNCLAINGRFLEEETKSRTTGTEEYQN 251
           ALTN+D C +G  +L VTD + P+   NVS ++ N LAIN        K     T   ++
Sbjct: 139 ALTNLDTCRAGFLELGVTDIVLPLMSNNVSNLLCNTLAIN--------KVPFNYTPPEKD 198

Query: 252 GFPTWLSTGDRKLLQSARVRAHLVVAKDGSGKFRTVQAAINAAARRRGRSRFVIYIKRGV 311
           GFP+W+  GDRKLLQS+  + + VVAKDGSG F+T++ AI+AA+   G  RFVIY+K+GV
Sbjct: 199 GFPSWVKPGDRKLLQSSTPKDNAVVAKDGSGNFKTIKEAIDAAS---GSGRFVIYVKQGV 258

Query: 312 YKENIEVGNDNANIMLVGDGIRFTPMIGS 339
           Y EN+E+     N+ML GDGI  T + GS
Sbjct: 259 YSENLEI--RKKNVMLRGDGIGKTIITGS 268

[Close]
BLAST of Cp4.1LG00g00270 vs. TAIR10
Match: AT4G00190.1 (AT4G00190.1 pectin methylesterase 38)

HSP 1 Score: 134.0 bits (336), Expect = 2.2e-31
Identity = 87/255 (34.12%), Postives = 137/255 (53.73%), Query Frame = 1 

Query: 84  NKWCNTTPHPKVCIFFMSPLKSPPQNRLQFRKLAIKTTLEGAAKAQNYVSRFGPTCKTTR 143
           N+ C+ TPHP  C   +   K P ++  QF +++++ TL+GA KA++      P   +  
Sbjct: 5   NEMCDETPHPGECKTLLIKHK-PIRSTTQFLQVSVERTLDGAVKAKSDTYFLEPQFGS-- 64

Query: 144 QRAAWTDCFNLYNDTVFQLNRTIQCLDAGTRLRHCTDFDAQTWLSSALTNIDMCSSGAAD 203
            + AW +C +LY  T+ +LN ++ C         C+  D Q WLS+ALTN+D C    ++
Sbjct: 65  -KQAWEECMDLYEQTIHRLNESVLCPK-----NVCSRSDVQAWLSTALTNLDTCQEEMSE 124

Query: 204 LNVTDFITPIKCQNVSKMISNCLAINGRFLEEETKSRTTGTEEYQNGFPTWLSTGDRKLL 263
           L V+        ++++  + N LAIN R                QNG    +S    K L
Sbjct: 125 LGVSSH----SLESITIDVINTLAINKRM--------------EQNGKEFGISKITMKTL 184

Query: 264 QSARVRAHLVVAKDGSGKFRTVQAAINAAARR-RGRSRFVIYIKRGVYKENIEVGNDNAN 323
            S   +  +VVA+DGSG ++T+Q A+N A  R +G  R+VI++K+GVY+E + VG  + N
Sbjct: 185 -SIGEKVDVVVAQDGSGDYKTIQEAVNGAGERLKGSPRYVIHVKQGVYEEYVNVGIKSNN 231

Query: 324 IMLVGDGIRFTPMIG 338
           IM+ GDGI  T + G
Sbjct: 245 IMITGDGIGKTIITG 231

[Close]
BLAST of Cp4.1LG00g00270 vs. NCBI nr
Match: gi|778676190|ref|XP_011650542.1| (PREDICTED: probable pectinesterase/pectinesterase inhibitor 59 [Cucumis sativus])

HSP 1 Score: 391.3 bits (1004), Expect = 2.2e-105
Identity = 202/288 (70.14%), Postives = 229/288 (79.51%), Query Frame = 1 

Query: 51  MAPHIQIAFFSIALFALLSNSSSSLTKTSANTINKWCNTTPHPKVCIFFMSPLKSPPQNR 110
           MA H +I F SI  F LLSN+       SAN I KWC TTPHP+VC+  MSP+K PPQNR
Sbjct: 1   MASHYKILFISITFFTLLSNN------VSANPIIKWCKTTPHPRVCVSLMSPIKPPPQNR 60

Query: 111 LQFRKLAIKTTLEGAAKAQNYVSRFGPTCKTTRQRAAWTDCFNLYNDTVFQLNRTIQCL- 170
            +FR++AI+TTLE AA+A+ Y +RFGPTCKT+RQR AWTDCF LYND V QLNRT+ C+ 
Sbjct: 61  REFRQMAIQTTLEKAAEARAYTARFGPTCKTSRQRTAWTDCFKLYNDVVLQLNRTLHCVV 120

Query: 171 -DAGTRLRHCTDFDAQTWLSSALTNIDMCSSGAADLNVTDFITPIKCQNVSKMISNCLAI 230
            D     R CTDFDAQTWLSSALT+ID+C+SGAADLNVTDFITPIKC NVSKMISNCLAI
Sbjct: 121 TDEAIHRRSCTDFDAQTWLSSALTDIDLCNSGAADLNVTDFITPIKCLNVSKMISNCLAI 180

Query: 231 NGRFLEEETKSRTTGTEEYQNG-FPTWLSTGDRKLLQS--ARVRAHLVVAKDGSGKFRTV 290
           NG FLEEE      G    +NG FP W+S GDRKLL+S   RVRA+LVVAKDGSG FR V
Sbjct: 181 NGGFLEEEGVKYDDG----RNGSFPMWVSEGDRKLLESRPGRVRANLVVAKDGSGTFRRV 240

Query: 291 QAAINAAARRRGRSRFVIYIKRGVYKENIEVGNDNANIMLVGDGIRFT 334
           QAAI+AAARRRGR RF+IY+KRGVY+ENIEVGNDN NIMLVGDG+RFT
Sbjct: 241 QAAIDAAARRRGRGRFIIYVKRGVYRENIEVGNDNGNIMLVGDGMRFT 278

[Close]
BLAST of Cp4.1LG00g00270 vs. NCBI nr
Match: gi|659102836|ref|XP_008452338.1| (PREDICTED: probable pectinesterase/pectinesterase inhibitor 59 [Cucumis melo])

HSP 1 Score: 383.3 bits (983), Expect = 5.9e-103
Identity = 204/290 (70.34%), Postives = 228/290 (78.62%), Query Frame = 1 

Query: 51  MAPHIQIAFF-SIAL-FALLSNSSSSLTKTSANTINKWCNTTPHPKVCIFFMSPLKSPPQ 110
           MA H +I  F SI L F +LSN+ S+      N I KWC TTPHPKVCI FMSP+K PPQ
Sbjct: 1   MASHYKIILFISITLLFTILSNNVSA-----NNPIIKWCKTTPHPKVCISFMSPIKPPPQ 60

Query: 111 NRLQFRKLAIKTTLEGAAKAQNYVSRFGPTCKTTRQRAAWTDCFNLYNDTVFQLNRTIQC 170
           NR +FR++AI+TTLE A +A+ Y +RFGPTCKTTRQR AWTDCF LYND VFQLNRT+QC
Sbjct: 61  NREEFRQVAIQTTLEKADEAKAYTARFGPTCKTTRQRTAWTDCFKLYNDVVFQLNRTLQC 120

Query: 171 LDAGTRLRH---CTDFDAQTWLSSALTNIDMCSSGAADLNVTDFITPIKCQNVSKMISNC 230
           L     +     CTDFDAQTWLSSALT+ID+C+SGAADLNVTDFI PIKC NVSKMISNC
Sbjct: 121 LVTDETIHRRPTCTDFDAQTWLSSALTDIDLCNSGAADLNVTDFIAPIKCLNVSKMISNC 180

Query: 231 LAINGRFLEEETKSRTTGTEEYQNGFPTWLSTGDRKLLQS--ARVRAHLVVAKDGSGKFR 290
           LAING FLEEE      G       FP W+S GDRKLL+S   RVRA+LVVAKDGSGKFR
Sbjct: 181 LAINGGFLEEEGVKYDDGG---NGSFPMWVSEGDRKLLESRPRRVRANLVVAKDGSGKFR 240

Query: 291 TVQAAINAAARRRGRSRFVIYIKRGVYKENIEVGNDNANIMLVGDGIRFT 334
            VQAAI+AAARRRGR RFVIY+KRGVY+ENIEVGNDN NIMLVGDG+RFT
Sbjct: 241 RVQAAIDAAARRRGRGRFVIYVKRGVYRENIEVGNDNGNIMLVGDGMRFT 282

[Close]
BLAST of Cp4.1LG00g00270 vs. NCBI nr
Match: gi|1009122303|ref|XP_015877930.1| (PREDICTED: probable pectinesterase/pectinesterase inhibitor 33 [Ziziphus jujuba])

HSP 1 Score: 267.7 bits (683), Expect = 3.6e-68
Identity = 145/283 (51.24%), Postives = 197/283 (69.61%), Query Frame = 1 

Query: 59  FFSIALFALLSNSSSSLTKTSANTINKWCNTTPHPKVCIFFMSPLKS--PPQNRLQFRKL 118
           F   ++F+L+    S+L++   + I+ WCN TP+P+ C +F++  +     ++  +FRK+
Sbjct: 10  FILASIFSLIF--PSALSRNHYSNISWWCNKTPNPEPCKYFLNQNRHRFAIKHTTEFRKV 69

Query: 119 AIKTTLEGAAKAQNYVSRFGPTCKTTRQRAAWTDCFNLYNDTVFQLNRTIQCLDAGTRLR 178
            ++  LE A  AQ + S FG  C+   Q+AAWTDC  LYNDTV QLNRT++ L    +  
Sbjct: 70  LVQIALERALDAQRHASEFGQNCQNKHQKAAWTDCLKLYNDTVSQLNRTLEGLGHNWK-- 129

Query: 179 HCTDFDAQTWLSSALTNIDMCSSGAADLNVTDFITPIKCQN-VSKMISNCLAINGRFLEE 238
            CTDFDAQTWLS+ALTNI  C +G+ +LNV DFI PI   N VS++ISN LAIN + L+ 
Sbjct: 130 -CTDFDAQTWLSTALTNIATCRAGSLELNVWDFILPIASNNNVSELISNTLAINSQLLDL 189

Query: 239 ETKSRTTGTEEYQNGFPTWLSTGDRKLLQSARVRAHLVVAKDGSGKFRTVQAAINAAARR 298
           + ++ T   +     FPTW+S  +RKLLQS+ ++AHLV+AKDGSG FRTVQAAINAA+RR
Sbjct: 190 KRENHTDDDD-----FPTWVSYHERKLLQSSSIKAHLVIAKDGSGHFRTVQAAINAASRR 249

Query: 299 RGRSRFVIYIKRGVYKENIEVGNDNANIMLVGDGIRFTPMIGS 339
           R  +RFVIYIK+G+Y ENI VGN+N NIMLVGDGI+ T +  S
Sbjct: 250 RRTTRFVIYIKKGIYWENIVVGNNNNNIMLVGDGIKNTIITAS 282

[Close]
BLAST of Cp4.1LG00g00270 vs. NCBI nr
Match: gi|567858026|ref|XP_006421696.1| (hypothetical protein CICLE_v10004719mg [Citrus clementina])

HSP 1 Score: 266.9 bits (681), Expect = 6.1e-68
Identity = 144/284 (50.70%), Postives = 195/284 (68.66%), Query Frame = 1 

Query: 54  HIQIAFFSIALFALLSNSSSSLTKTSANTINKWCNTTPHPKVCIFFMSPL--KSPPQNRL 113
           +I +    I+L ++  ++ S   + SA  I  WC+ TPHP+ C +F+S    +   ++R 
Sbjct: 5   NISLLIIFISLSSIFYHALSR--RPSATNITWWCSKTPHPEPCNYFLSRSHHRYTFKHRS 64

Query: 114 QFRKLAIKTTLEGAAKAQNYVSRFGPTCKTTRQRAAWTDCFNLYNDTVFQLNRTIQCLDA 173
            FRK+ ++  +E A KA + V  FG  C+   Q+AAW+DC  LY +TV QLNRT+Q L +
Sbjct: 65  DFRKIIVQVAIEQAHKAHDQVMEFGTNCENKHQKAAWSDCVKLYANTVLQLNRTLQGLKS 124

Query: 174 GTRLRHCTDFDAQTWLSSALTNIDMCSSGAADLNVTDFITPIKCQNVSKMISNCLAINGR 233
               R CTDFDAQTWLS+ALTNI  C SG  +LNV+DFITP+   N+S++ISN LA+NG 
Sbjct: 125 D---RSCTDFDAQTWLSTALTNIRTCQSGYMELNVSDFITPVMSNNLSQLISNSLAVNGV 184

Query: 234 FLEEETKSRTTGTEEYQNGFPTWLSTGDRKLLQSARV--RAHLVVAKDGSGKFRTVQAAI 293
            L+ E  +       Y NGFP+WLS  +R LL+S+ +  RA+LVVA DGSG +RT+QAAI
Sbjct: 185 LLKRENVT-------YTNGFPSWLSGHERNLLESSSLEARANLVVATDGSGNYRTIQAAI 244

Query: 294 NAAARRRGRSRFVIYIKRGVYKENIEVGNDNANIMLVGDGIRFT 334
           NAAARRRG  RF+I++KRGVY+ENIEVG +N NIMLVG+G+R T
Sbjct: 245 NAAARRRGSGRFIIHVKRGVYRENIEVGLNNNNIMLVGEGMRNT 276

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BLAST of Cp4.1LG00g00270 vs. NCBI nr
Match: gi|729382763|ref|XP_010550793.1| (PREDICTED: probable pectinesterase/pectinesterase inhibitor 33 [Tarenaya hassleriana])

HSP 1 Score: 266.2 bits (679), Expect = 1.0e-67
Identity = 140/258 (54.26%), Postives = 183/258 (70.93%), Query Frame = 1 

Query: 86  WCNTTPHPKVCIFFMS----PLKSPPQNRLQFRKLAIKTTLEGAAKAQNYVSRFGPTCKT 145
           WC+ TPHP+ C ++      P K PP+ R +FR+  ++  L+ A   Q+   RFGP+C  
Sbjct: 22  WCDKTPHPEPCKYYFKKSPRPHKPPPRFRSEFRRRLVELALDHAETTQSQTVRFGPSCTN 81

Query: 146 TRQRAAWTDCFNLYNDTVFQLNRTIQCLDAGTRLR-HCTDFDAQTWLSSALTNIDMCSSG 205
            R RAAW+DC  LY+DTV QLNRT++ LD+       CTDFDAQTWLS+A TNID C  G
Sbjct: 82  KRSRAAWSDCVKLYDDTVVQLNRTLRGLDSTAPADVRCTDFDAQTWLSTAQTNIDTCRLG 141

Query: 206 AADLNVTDFITP-IKCQNVSKMISNCLAINGRFLEEETKSRTTGTEEYQNGFPTWLSTGD 265
           + DLNV+DF+ P I  +N+S +ISNCLA+NG  +++ + +RTT    Y+  FP+W+S  +
Sbjct: 142 SEDLNVSDFVLPAISNKNLSDLISNCLAVNGALMKQHS-NRTTA--NYREHFPSWISRHE 201

Query: 266 RKLLQS---ARVRAHLVVAKDGSGKFRTVQAAINAAARRRGRS-RFVIYIKRGVYKENIE 325
           R+LL S   AR  A+LVVA+DGSG FR++QAAIN AARRR  + RFVIYIK+GVY+ENIE
Sbjct: 202 RRLLLSTSMARAMANLVVARDGSGHFRSIQAAINVAARRRSMAKRFVIYIKKGVYRENIE 261

Query: 326 VGNDNANIMLVGDGIRFT 334
           VGNDN N+MLVGDG R T
Sbjct: 262 VGNDNDNVMLVGDGARTT 276

The following BLAST results are available for this feature:
BLAST of Cp4.1LG00g00270 vs. Swiss-Prot
Match NameE-valueIdentityDescription
PME33_ARATH6.3e-6549.81Probable pectinesterase/pectinesterase inhibitor 33 OS=Arabidopsis thaliana GN=P... [more]
PME60_ARATH1.2e-6047.06Probable pectinesterase/pectinesterase inhibitor 60 OS=Arabidopsis thaliana GN=P... [more]
PME59_ARATH8.9e-5945.58Probable pectinesterase/pectinesterase inhibitor 59 OS=Arabidopsis thaliana GN=P... [more]
PME17_ARATH8.0e-5241.64Probable pectinesterase/pectinesterase inhibitor 17 OS=Arabidopsis thaliana GN=P... [more]
PME2_CITSI1.6e-4741.43Pectinesterase 2 OS=Citrus sinensis GN=PECS-2.1 PE=2 SV=1[more]
BLAST of Cp4.1LG00g00270 vs. TrEMBL
Match NameE-valueIdentityDescription
A0A0A0L330_CUCSA1.5e-10570.14Pectinesterase OS=Cucumis sativus GN=Csa_3G088980 PE=4 SV=1[more]
V4SD84_9ROSI4.3e-6850.70Pectinesterase OS=Citrus clementina GN=CICLE_v10004719mg PE=4 SV=1[more]
A0A067FDF5_CITSI2.1e-6752.87Pectinesterase (Fragment) OS=Citrus sinensis GN=CISIN_1g0366962mg PE=4 SV=1[more]
W9SDY8_9ROSA2.1e-6751.56Pectinesterase OS=Morus notabilis GN=L484_001126 PE=4 SV=1[more]
A0A061FZE1_THECC4.0e-6647.44Pectinesterase OS=Theobroma cacao GN=TCM_014943 PE=4 SV=1[more]
BLAST of Cp4.1LG00g00270 vs. TAIR10
Match NameE-valueIdentityDescription
AT3G47400.13.6e-6649.81 Plant invertase/pectin methylesterase inhibitor superfamily[more]
AT5G51500.17.0e-6247.06 Plant invertase/pectin methylesterase inhibitor superfamily[more]
AT5G51490.15.0e-6045.58 Plant invertase/pectin methylesterase inhibitor superfamily[more]
AT2G45220.14.5e-5341.64 Plant invertase/pectin methylesterase inhibitor superfamily[more]
AT4G00190.12.2e-3134.12 pectin methylesterase 38[more]
BLAST of Cp4.1LG00g00270 vs. NCBI nr
Match NameE-valueIdentityDescription
gi|778676190|ref|XP_011650542.1|2.2e-10570.14PREDICTED: probable pectinesterase/pectinesterase inhibitor 59 [Cucumis sativus][more]
gi|659102836|ref|XP_008452338.1|5.9e-10370.34PREDICTED: probable pectinesterase/pectinesterase inhibitor 59 [Cucumis melo][more]
gi|1009122303|ref|XP_015877930.1|3.6e-6851.24PREDICTED: probable pectinesterase/pectinesterase inhibitor 33 [Ziziphus jujuba][more]
gi|567858026|ref|XP_006421696.1|6.1e-6850.70hypothetical protein CICLE_v10004719mg [Citrus clementina][more]
gi|729382763|ref|XP_010550793.1|1.0e-6754.26PREDICTED: probable pectinesterase/pectinesterase inhibitor 33 [Tarenaya hassler... [more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: Molecular Function
TermDefinition
GO:0004857enzyme inhibitor activity
GO:0030599pectinesterase activity
Vocabulary: Biological Process
TermDefinition
GO:0042545cell wall modification
Vocabulary: Cellular Component
TermDefinition
GO:0005618cell wall
Vocabulary: INTERPRO
TermDefinition
IPR018040Pectinesterase_Tyr_AS
IPR012334Pectin_lyas_fold
IPR011050Pectin_lyase_fold/virulence
IPR006501Pectinesterase_inhib_dom
IPR000070Pectinesterase_cat
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0042545 cell wall modification
biological_process GO:0005982 starch metabolic process
biological_process GO:0005985 sucrose metabolic process
biological_process GO:0043086 negative regulation of catalytic activity
biological_process GO:0045490 pectin catabolic process
cellular_component GO:0005618 cell wall
cellular_component GO:0005576 extracellular region
molecular_function GO:0004857 enzyme inhibitor activity
molecular_function GO:0030599 pectinesterase activity
molecular_function GO:0045330 aspartyl esterase activity
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG00g00270.1Cp4.1LG00g00270.1mRNA


InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo
Date Performed: 2017-12-02
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000070Pectinesterase, catalyticPFAMPF01095Pectinesterasecoord: 334..425
score: 2.6
IPR006501Pectinesterase inhibitor domainGENE3DG3DSA:1.20.140.40coord: 81..232
score: 5.0
IPR006501Pectinesterase inhibitor domainPFAMPF04043PMEIcoord: 82..228
score: 1.2
IPR006501Pectinesterase inhibitor domainSMARTSM00856PMEI_2coord: 78..228
score: 2.0
IPR006501Pectinesterase inhibitor domainTIGRFAMsTIGR01614TIGR01614coord: 60..229
score: 8.2
IPR006501Pectinesterase inhibitor domainunknownSSF101148Plant invertase/pectin methylesterase inhibitorcoord: 81..232
score: 2.22
IPR011050Pectin lyase fold/virulence factorunknownSSF51126Pectin lyase-likecoord: 268..438
score: 1.69
IPR012334Pectin lyase foldGENE3DG3DSA:2.160.20.10coord: 338..437
score: 3.6E-30coord: 268..331
score: 1.2
IPR018040Pectinesterase, Tyr active sitePROSITEPS00800PECTINESTERASE_1coord: 297..316
scor
NoneNo IPR availablePANTHERPTHR31707FAMILY NOT NAMEDcoord: 50..440
score: 3.4E
NoneNo IPR availablePANTHERPTHR31707:SF45PECTINESTERASE/PECTINESTERASE INHIBITOR 33-RELATEDcoord: 50..440
score: 3.4E

Synteny
The following gene(s) are orthologous to this gene:
GeneOrthologueOrganismBlock
Cp4.1LG00g00270CmaCh16G008360Cucurbita maxima (Rimu)cmacpeB337
Cp4.1LG00g00270CmoCh16G009260Cucurbita moschata (Rifu)cmocpeB301
Cp4.1LG00g00270Cla003829Watermelon (97103) v1cpewmB002
Cp4.1LG00g00270Csa3G088980Cucumber (Chinese Long) v2cpecuB009
Cp4.1LG00g00270MELO3C016609Melon (DHL92) v3.5.1cpemeB014
Cp4.1LG00g00270ClCG10G006080Watermelon (Charleston Gray)cpewcgB001
Cp4.1LG00g00270CSPI03G05560Wild cucumber (PI 183967)cpecpiB007
Cp4.1LG00g00270Lsi05G006470Bottle gourd (USVL1VR-Ls)cpelsiB014
Cp4.1LG00g00270MELO3C016609.2Melon (DHL92) v3.6.1cpemedB015
Cp4.1LG00g00270CsaV3_3G005650Cucumber (Chinese Long) v3cpecucB0011
Cp4.1LG00g00270Bhi11G000548Wax gourdcpewgoB0000
Cp4.1LG00g00270CsGy3G005690Cucumber (Gy14) v2cgybcpeB280
Cp4.1LG00g00270Carg24835Silver-seed gourdcarcpeB1109
The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:

None