CmaCh16G008360 (gene) Cucurbita maxima (Rimu)

BLAST of CmaCh16G008360 vs. Swiss-Prot
Match: PME33_ARATH (Probable pectinesterase/pectinesterase inhibitor 33 OS=Arabidopsis thaliana GN=PME33 PE=2 SV=1)

HSP 1 Score: 598.2 bits (1541), Expect = 8.8e-170
Identity = 298/511 (58.32%), Postives = 383/511 (74.95%), Query Frame = 1

Query: 36  WCNTTPHPKVCIFFMSPLKS------PPQNRIEFRKMAIKTTLEGAAKAQNYVSRFGPTC 95
           WCN TPH + C ++            PP+ R EF +M +K  L+ A    +   +FGP+C
Sbjct: 87  WCNKTPHAETCNYYFRKSSQNNINLRPPRFRSEFLRMLVKVALDQAVITHSQTVKFGPSC 146

Query: 96  KTTRQRAAWTDCFNLYNDTVFQLNRTIRCLH-AGTRLRHCTDFDAQTWLSSALTNIDMCS 155
              +++AAW+DC NL+ +TV QLNRT++ L+ A +    CTDFDAQTWLS+A TNI+ C 
Sbjct: 147 TNNQRKAAWSDCVNLFQNTVAQLNRTLKGLNPAASSDVKCTDFDAQTWLSTAQTNIETCR 206

Query: 156 SGAADLNVTDFITP-IKCQNVSRMISNCLAINGRFLEGETKSRTTGAEEYQNGFPRWVSS 215
           SG+ DLNV+DF+ P I  +N+S +I NCLA+NG  ++    + T   +EY   FP WVS 
Sbjct: 207 SGSEDLNVSDFVMPVISNKNLSDLIGNCLAVNGVLMKQHDHTTTANHKEY---FPSWVSR 266

Query: 216 GDRKLLQSARVRA---HLVVAKDGSGKFRTVQAAINAAARRRGRSRFVIYIKRGVYRENI 275
            +R+LL SA +     HLVVA+D SG FR++QAAIN AARRR +SRFVIY+K+GVYRENI
Sbjct: 267 HERRLLVSASLAKSSPHLVVAQDRSGHFRSIQAAINFAARRRFKSRFVIYVKKGVYRENI 326

Query: 276 EIGNDNANIMLVGDGIRFTVITSGRSVAAGFTTFNSATAGIQGPGFMARGIRFVNTAGPR 335
           ++GNDN NIMLVGDG R T+ITSGRSV  G+TT+NSAT G  G  F+A+ + F+NTAGP 
Sbjct: 327 DVGNDNHNIMLVGDGERKTIITSGRSVQHGYTTYNSATGGFGGQRFVAKDMTFINTAGPL 386

Query: 336 MGQAVALRSSSDLSVFHRCSFEGYQDTLMVLSQRQFYKQCYVYGTIDFIFGNAAVVLQNC 395
            GQAVA+RSSSDLSVF+R    G+QDTL + SQRQF+++CY+ GTIDFIFGNAAVV QNC
Sbjct: 387 RGQAVAVRSSSDLSVFYRVGIHGFQDTLYIHSQRQFFRECYISGTIDFIFGNAAVVFQNC 446

Query: 396 LIYVRRPLKGQVNVITAQGREDPFQNSGISIHNSQIRAAADLQPMIGSVKTYLGRPWKKY 455
           +I VRRPL GQ N+ITAQGR DPFQN+GI+IH+S+I AA+DL+P+I + KTYLGRPW+ Y
Sbjct: 447 MILVRRPLHGQANIITAQGRGDPFQNTGITIHSSRIIAASDLKPVIRAYKTYLGRPWQAY 506

Query: 456 SRTVIMRSYIDSLVSPMGWLAW-QGTRFAQDTLYYGEYRNFGPRASTRLRVRWPGFHAIT 515
           SR  IM++YID+ +SP+GW  W +G+ FA +T++YGEY+NFGP +STR RVRW GFHAIT
Sbjct: 507 SRVTIMKTYIDNSISPLGWSPWLRGSNFALNTVFYGEYKNFGPGSSTRWRVRWKGFHAIT 566

Query: 516 TRNVASKFTVRRLIAGHTWLPATGVPFKLDL 535
           +  VAS+FTV  LIAG +WLPATGVPFK  L
Sbjct: 567 STAVASRFTVGSLIAGGSWLPATGVPFKSGL 594

BLAST of CmaCh16G008360 vs. Swiss-Prot
Match: PME59_ARATH (Probable pectinesterase/pectinesterase inhibitor 59 OS=Arabidopsis thaliana GN=PME59 PE=2 SV=1)

HSP 1 Score: 577.0 bits (1486), Expect = 2.1e-163
Identity = 299/538 (55.58%), Postives = 381/538 (70.82%), Query Frame = 1

Query: 1   MAPHIQIAFFSIALFALLSNSSSSPTKTSAN-TINKWCNTTPHPKVCIFFMSPLKS--PP 60
           M   + I F  + L  LL      P  T A+     WC+ TP+P  C  +         P
Sbjct: 4   MMQKLSILFLHLILLVLLC---VHPLTTVADRNSTDWCDKTPYPDPCKCYFKNHNGFQQP 63

Query: 61  QNRIEFRKMAIKTTLEGAAKAQNYVSRFGPTCKTTRQRAAWTDCFNLYNDTVFQLNRTIR 120
               EFR M ++  ++ A  A+  ++  G  C  ++++A   DC +LY DT+ QLNRT+ 
Sbjct: 64  TQLSEFRVMLVEAAMDRAISARAELTNSGKNCTDSKKQAVLADCIDLYGDTIMQLNRTLH 123

Query: 121 CLH--AGTRLRHCTDFDAQTWLSSALTNIDMCSSGAADLNVTDFITPIKCQ-NVSRMISN 180
            +   AG   + CTDFDAQTWLS+ALTN + C  G++DLNVTDFITPI     +S +ISN
Sbjct: 124 GVSPKAGAA-KSCTDFDAQTWLSTALTNTETCRRGSSDLNVTDFITPIVSNTKISHLISN 183

Query: 181 CLAINGRFLEGETKSRTTGAEEYQNGFPRWVSSGDRKLLQSARVRAHLVVAKDGSGKFRT 240
           CLA+NG  L    K  TT     Q GFP W+S  D++LL++  VRA+LVVAKDGSG F T
Sbjct: 184 CLAVNGALLTAGNKGNTTA---NQKGFPTWLSRKDKRLLRA--VRANLVVAKDGSGHFNT 243

Query: 241 VQAAINAAARRRGRS-RFVIYIKRGVYRENIEIGNDNANIMLVGDGIRFTVITSGRSVAA 300
           VQAAI+ A RR+  S RFVIY+KRG+Y+ENI +  +N +IMLVGDG+R T+IT GRSV  
Sbjct: 244 VQAAIDVAGRRKVTSGRFVIYVKRGIYQENINVRLNNDDIMLVGDGMRSTIITGGRSVQG 303

Query: 301 GFTTFNSATAGIQGPGFMARGIRFVNTAGPRMGQAVALRSSSDLSVFHRCSFEGYQDTLM 360
           G+TT+NSATAGI+G  F+A+GI F NTAGP  GQAVALRSSSDLS+F++CS EGYQDTLM
Sbjct: 304 GYTTYNSATAGIEGLHFIAKGITFRNTAGPAKGQAVALRSSSDLSIFYKCSIEGYQDTLM 363

Query: 361 VLSQRQFYKQCYVYGTIDFIFGNAAVVLQNCLIYVRRPLKGQVNVITAQGREDPFQNSGI 420
           V SQRQFY++CY+YGT+DFIFGNAA V QNCLI  RRPLKGQ NVITAQGR DPFQN+GI
Sbjct: 364 VHSQRQFYRECYIYGTVDFIFGNAAAVFQNCLILPRRPLKGQANVITAQGRADPFQNTGI 423

Query: 421 SIHNSQIRAAADLQPMIGSVKTYLGRPWKKYSRTVIMRSYIDSLVSPMGWLAW-QGTRFA 480
           SIHNS+I  A DL+P++G+VKTY+GRPW K+SRTV++++Y+D++VSP+GW  W +G+ F 
Sbjct: 424 SIHNSRILPAPDLKPVVGTVKTYMGRPWMKFSRTVVLQTYLDNVVSPVGWSPWIEGSVFG 483

Query: 481 QDTLYYGEYRNFGPRASTRLRVRWPGFHAITTRNVASKFTVRRLIAGHTWLPATGVPF 531
            DTL+Y EY+N GP +STR RV W GFH +   + AS FTV + IAG  WLP TG+PF
Sbjct: 484 LDTLFYAEYKNTGPASSTRWRVSWKGFHVLGRASDASAFTVGKFIAGTAWLPRTGIPF 532

BLAST of CmaCh16G008360 vs. Swiss-Prot
Match: PME60_ARATH (Probable pectinesterase/pectinesterase inhibitor 60 OS=Arabidopsis thaliana GN=PME60 PE=2 SV=1)

HSP 1 Score: 576.2 bits (1484), Expect = 3.6e-163
Identity = 296/537 (55.12%), Postives = 385/537 (71.69%), Query Frame = 1

Query: 7   IAFFSIALFALLS---NSSSSPTKTSANTINKWCNTTPHPKVCIFFM---SPLKSPPQNR 66
           I+F S+ L  +L       ++    ++  I+ WC+ TP+P  C  +    S  + P Q  
Sbjct: 9   ISFLSLHLLLILLLCLRPLTTVADGNSTNIDGWCDKTPYPYPCKRYFIKHSGFRLPTQIS 68

Query: 67  IEFRKMAIKTTLEGAAKAQNYVSRFGPTCKTTRQRAAWTDCFNLYNDTVFQLNRTIRCLH 126
            EFR + ++  ++ A  A + ++     C   +++A   DC NLY DTV QLNRT++ + 
Sbjct: 69  -EFRVLLVEAAMDRAVSAWDKLTNSSKNCTDFKKQAVLADCINLYGDTVMQLNRTLQGVS 128

Query: 127 AGTRLRHCTDFDAQTWLSSALTNIDMCSSGAADLNVTDFITPIKCQN-VSRMISNCLAIN 186
           + T  R CTDFDAQTWLS+ALTN + C  G++DLNV+DF TPI     +S +ISNCLA+N
Sbjct: 129 SKTG-RRCTDFDAQTWLSTALTNTETCRRGSSDLNVSDFTTPIVSNTKISHLISNCLAVN 188

Query: 187 GRFLEGETKSRTTGAEEYQNGFPRWVSSGDRKLLQSARVRAHLVVAKDGSGKFRTVQAAI 246
           G  L       TTG  +   GFP WVS  +R+LLQ   VRA+LVVAKDGSG F+TVQAAI
Sbjct: 189 GALLTAGKNDSTTGDSK---GFPTWVSRKERRLLQLQSVRANLVVAKDGSGHFKTVQAAI 248

Query: 247 NAAARRRGRS-RFVIYIKRGVYRENIEIGNDNANIMLVGDGIRFTVITSGRSVAAGFTTF 306
           + A RR+  S RFVIY+KRG+Y+EN+ +  +N NIMLVGDG+R+T+IT GRSV  G+TT+
Sbjct: 249 DVAGRRKVTSGRFVIYVKRGIYQENLNVRLNNDNIMLVGDGMRYTIITGGRSVKGGYTTY 308

Query: 307 NSATAGIQGPGFMARGIRFVNTAGPRMGQAVALRSSSDLSVFHRCSFEGYQDTLMVLSQR 366
           +SATAGI+G  F+A+GI F NTAGP  GQAVALRSSSDLS+F+RCS EGYQDTLMV SQR
Sbjct: 309 SSATAGIEGLHFIAKGIAFQNTAGPAKGQAVALRSSSDLSIFYRCSIEGYQDTLMVHSQR 368

Query: 367 QFYKQCYVYGTIDFIFGNAAVVLQNCLIYVRRPLKGQVNVITAQGREDPFQNSGISIHNS 426
           QFY++CY+YGT+DFIFGNAAVV QNC+I  R PLKGQ NVITAQGR D FQN+GISIHNS
Sbjct: 369 QFYRECYIYGTVDFIFGNAAVVFQNCIILPRLPLKGQANVITAQGRTDLFQNTGISIHNS 428

Query: 427 QIRAAADLQPMIGSVKTYLGRPWKKYSRTVIMRSYIDSLVSPMGWLAW-QGTRFAQDTLY 486
            I  A DL+P++ SVKTY+GRPW  YSRTV++++YIDS+VSP+GW  W +G+ +  DTL+
Sbjct: 429 IIIPAPDLKPVVRSVKTYMGRPWMMYSRTVVLKTYIDSVVSPVGWSPWTKGSTYGLDTLF 488

Query: 487 YGEYRNFGPRASTRLRVRWPGFHAITTRNVASKFTVRRLIAGHTWLPATGVPFKLDL 535
           Y EY+N GP +STR RVRW GFH ++  + AS F+V + IAG  WLP +G+PF  +L
Sbjct: 489 YAEYKNIGPASSTRWRVRWKGFHVLSKASDASAFSVGKFIAGTAWLPGSGIPFTSEL 540

BLAST of CmaCh16G008360 vs. Swiss-Prot
Match: PME17_ARATH (Probable pectinesterase/pectinesterase inhibitor 17 OS=Arabidopsis thaliana GN=PME17 PE=2 SV=2)

HSP 1 Score: 504.2 bits (1297), Expect = 1.7e-141
Identity = 262/500 (52.40%), Postives = 343/500 (68.60%), Query Frame = 1

Query: 33  INKWCNTTPHPKVCIFFMSPLKS--PPQNRIEFRKMAIKTTLEGAAKAQNYVSRFGPTCK 92
           +  WC+ TP+PK C +F++   +  P ++  EF K+++K  L+ A  A+ +    GP C+
Sbjct: 30  VKAWCSQTPNPKPCEYFLTHNSNNEPIKSESEFLKISMKLVLDRAILAKTHAFTLGPKCR 89

Query: 93  TTRQRAAWTDCFNLYNDTVFQLNRTIRCLHAGTRLRHCTDFDAQTWLSSALTNIDMCSSG 152
            TR++AAW DC  LY+ TV ++N T+        ++ C+  DAQTWLS+ALTN+D C +G
Sbjct: 90  DTREKAAWEDCIKLYDLTVSKINETM-----DPNVK-CSKLDAQTWLSTALTNLDTCRAG 149

Query: 153 AADLNVTDFITPIKCQNVSRMISNCLAINGRFLEGETKSRTTGAEEYQNGFPRWVSSGDR 212
             +L VTD + P+   NVS ++ N LAIN        K         ++GFP WV  GDR
Sbjct: 150 FLELGVTDIVLPLMSNNVSNLLCNTLAIN--------KVPFNYTPPEKDGFPSWVKPGDR 209

Query: 213 KLLQSARVRAHLVVAKDGSGKFRTVQAAINAAARRRGRSRFVIYIKRGVYRENIEIGNDN 272
           KLLQS+  + + VVAKDGSG F+T++ AI+AA+   G  RFVIY+K+GVY EN+EI    
Sbjct: 210 KLLQSSTPKDNAVVAKDGSGNFKTIKEAIDAAS---GSGRFVIYVKQGVYSENLEI--RK 269

Query: 273 ANIMLVGDGIRFTVITSGRSVAAGFTTFNSATAGIQGPGFMARGIRFVNTAGPRMGQAVA 332
            N+ML GDGI  T+IT  +SV  G TTFNSAT    G GF+ARGI F NTAG    QAVA
Sbjct: 270 KNVMLRGDGIGKTIITGSKSVGGGTTTFNSATVAAVGDGFIARGITFRNTAGASNEQAVA 329

Query: 333 LRSSSDLSVFHRCSFEGYQDTLMVLSQRQFYKQCYVYGTIDFIFGNAAVVLQNCLIYVRR 392
           LRS SDLSVF++CSFE YQDTL V S RQFY+ C VYGT+DFIFGNAA VLQNC I+ RR
Sbjct: 330 LRSGSDLSVFYQCSFEAYQDTLYVHSNRQFYRDCDVYGTVDFIFGNAAAVLQNCNIFARR 389

Query: 393 PLKGQVNVITAQGREDPFQNSGISIHNSQIRAAADLQPMIGSVKTYLGRPWKKYSRTVIM 452
           P + + N ITAQGR DP QN+GI IHNS++ AA+DL+P++GS KTYLGRPW++YSRTV M
Sbjct: 390 P-RSKTNTITAQGRSDPNQNTGIIIHNSRVTAASDLRPVLGSTKTYLGRPWRQYSRTVFM 449

Query: 453 RSYIDSLVSPMGWLAWQGTRFAQDTLYYGEYRNFGPRASTRLRVRWPGFHAITTRNVASK 512
           ++ +DSL+ P GWL W G  FA  TL+Y E++N GP AST  RV WPGF  + + + ASK
Sbjct: 450 KTSLDSLIDPRGWLEWDG-NFALKTLFYAEFQNTGPGASTSGRVTWPGFRVLGSASEASK 507

Query: 513 FTVRRLIAGHTWLPATGVPF 531
           FTV   +AG +W+P++ VPF
Sbjct: 510 FTVGTFLAGGSWIPSS-VPF 507

BLAST of CmaCh16G008360 vs. Swiss-Prot
Match: PME2_CITSI (Pectinesterase 2 OS=Citrus sinensis GN=PECS-2.1 PE=2 SV=1)

HSP 1 Score: 492.7 bits (1267), Expect = 5.2e-138
Identity = 260/522 (49.81%), Postives = 344/522 (65.90%), Query Frame = 1

Query: 11  SIALFALLSNSSSSPTKTSANTINKWCNTTPHPKVCIFFMSPLKSPPQNR--IEFRKMAI 70
           S+ LF+L   S       S   +  WC  TP+P+ C +F++        +   +F K+++
Sbjct: 10  SLVLFSLSHTSFGY----SPEEVKSWCGKTPNPQPCEYFLTQKTDVTSIKQDTDFYKISL 69

Query: 71  KTTLEGAAKAQNYVSRFGPTCKTTRQRAAWTDCFNLYNDTVFQLNRTIRCLHAGTRLRHC 130
           +  LE A  AQ+     G  C+  R++AAW DC  LY  TV +LN+T            C
Sbjct: 70  QLALERATTAQSRTYTLGSKCRNEREKAAWEDCRELYELTVLKLNQTSNSSPG------C 129

Query: 131 TDFDAQTWLSSALTNIDMCSSGAADLNVTDFITPIKCQNVSRMISNCLAINGRFLEGETK 190
           T  D QTWLS+ALTN++ C +   DL V +++ P+   NV+++ISN L++N         
Sbjct: 130 TKVDKQTWLSTALTNLETCRASLEDLGVPEYVLPLLSNNVTKLISNTLSLN--------- 189

Query: 191 SRTTGAEEYQNGFPRWVSSGDRKLLQSARVRAHLVVAKDGSGKFRTVQAAINAAARRRGR 250
                   Y++GFP WV  GDRKLLQ+   RA++VVA+DGSG  +T+Q A+ AAA R G 
Sbjct: 190 KVPYNEPSYKDGFPTWVKPGDRKLLQTT-PRANIVVAQDGSGNVKTIQEAV-AAASRAGG 249

Query: 251 SRFVIYIKRGVYRENIEIGNDNANIMLVGDGIRFTVITSGRSVAAGFTTFNSATAGIQGP 310
           SR+VIYIK G Y ENIE+     NIM VGDGI  T+IT  +SV  G TTF SAT  + G 
Sbjct: 250 SRYVIYIKAGTYNENIEV--KLKNIMFVGDGIGKTIITGSKSVGGGATTFKSATVAVVGD 309

Query: 311 GFMARGIRFVNTAGPRMGQAVALRSSSDLSVFHRCSFEGYQDTLMVLSQRQFYKQCYVYG 370
            F+AR I   NTAGP   QAVALRS SDLSVF+RCSFEGYQDTL V SQRQFY++C +YG
Sbjct: 310 NFIARDITIRNTAGPNNHQAVALRSGSDLSVFYRCSFEGYQDTLYVHSQRQFYRECDIYG 369

Query: 371 TIDFIFGNAAVVLQNCLIYVRRPLKGQVNVITAQGREDPFQNSGISIHNSQIRAAADLQP 430
           T+DFIFGNAAVVLQNC I+ R+P   + N +TAQGR DP Q++GI IHN ++ AA+DL+P
Sbjct: 370 TVDFIFGNAAVVLQNCNIFARKP-PNRTNTLTAQGRTDPNQSTGIIIHNCRVTAASDLKP 429

Query: 431 MIGSVKTYLGRPWKKYSRTVIMRSYIDSLVSPMGWLAWQGTRFAQDTLYYGEYRNFGPRA 490
           +  SVKT+LGRPWK+YSRTV +++++DSL++P GW+ W G  FA +TLYY EY N GP +
Sbjct: 430 VQSSVKTFLGRPWKQYSRTVYIKTFLDSLINPAGWMEWSGD-FALNTLYYAEYMNTGPGS 489

Query: 491 STRLRVRWPGFHAITTRNVASKFTVRRLIAGHTWLPATGVPF 531
           ST  RV+W G+H +T+ +  S+FTV   IAG++WLPAT VPF
Sbjct: 490 STANRVKWRGYHVLTSPSQVSQFTVGNFIAGNSWLPATNVPF 506

BLAST of CmaCh16G008360 vs. TrEMBL
Match: A0A0A0L330_CUCSA (Pectinesterase OS=Cucumis sativus GN=Csa_3G088980 PE=4 SV=1)

HSP 1 Score: 852.8 bits (2202), Expect = 2.2e-244
Identity = 429/537 (79.89%), Postives = 466/537 (86.78%), Query Frame = 1

Query: 1   MAPHIQIAFFSIALFALLSNSSSSPTKTSANTINKWCNTTPHPKVCIFFMSPLKSPPQNR 60
           MA H +I F SI  F LLSN+       SAN I KWC TTPHP+VC+  MSP+K PPQNR
Sbjct: 1   MASHYKILFISITFFTLLSNN------VSANPIIKWCKTTPHPRVCVSLMSPIKPPPQNR 60

Query: 61  IEFRKMAIKTTLEGAAKAQNYVSRFGPTCKTTRQRAAWTDCFNLYNDTVFQLNRTIRCLH 120
            EFR+MAI+TTLE AA+A+ Y +RFGPTCKT+RQR AWTDCF LYND V QLNRT+ C+ 
Sbjct: 61  REFRQMAIQTTLEKAAEARAYTARFGPTCKTSRQRTAWTDCFKLYNDVVLQLNRTLHCVV 120

Query: 121 AGTRL--RHCTDFDAQTWLSSALTNIDMCSSGAADLNVTDFITPIKCQNVSRMISNCLAI 180
               +  R CTDFDAQTWLSSALT+ID+C+SGAADLNVTDFITPIKC NVS+MISNCLAI
Sbjct: 121 TDEAIHRRSCTDFDAQTWLSSALTDIDLCNSGAADLNVTDFITPIKCLNVSKMISNCLAI 180

Query: 181 NGRFLEGETKSRTTGAEEYQNG-FPRWVSSGDRKLLQS--ARVRAHLVVAKDGSGKFRTV 240
           NG FLE E      G    +NG FP WVS GDRKLL+S   RVRA+LVVAKDGSG FR V
Sbjct: 181 NGGFLEEEGVKYDDG----RNGSFPMWVSEGDRKLLESRPGRVRANLVVAKDGSGTFRRV 240

Query: 241 QAAINAAARRRGRSRFVIYIKRGVYRENIEIGNDNANIMLVGDGIRFTVITSGRSVAAGF 300
           QAAI+AAARRRGR RF+IY+KRGVYRENIE+GNDN NIMLVGDG+RFTVITSGRSVAAGF
Sbjct: 241 QAAIDAAARRRGRGRFIIYVKRGVYRENIEVGNDNGNIMLVGDGMRFTVITSGRSVAAGF 300

Query: 301 TTFNSATAGIQGPGFMARGIRFVNTAGPRMGQAVALRSSSDLSVFHRCSFEGYQDTLMVL 360
           TTF+SATAGIQGPGF+AR IRFVNTAGPRMGQAVALRSSSDLSVFHRCSFEGYQDTLMVL
Sbjct: 301 TTFSSATAGIQGPGFIARDIRFVNTAGPRMGQAVALRSSSDLSVFHRCSFEGYQDTLMVL 360

Query: 361 SQRQFYKQCYVYGTIDFIFGNAAVVLQNCLIYVRRPLKGQVNVITAQGREDPFQNSGISI 420
           SQRQFYKQCYVYGTIDFIFGNAAVVLQNC+IYVRRPLKGQVNVITAQGREDPFQNSGISI
Sbjct: 361 SQRQFYKQCYVYGTIDFIFGNAAVVLQNCMIYVRRPLKGQVNVITAQGREDPFQNSGISI 420

Query: 421 HNSQIRAAADLQPMIGSVKTYLGRPWKKYSRTVIMRSYIDSLVSPMGWLAWQGTRFAQDT 480
           HNSQIRAAADL+PM+GSVKTYLGRPWKKYSRTVIMRSYID LVSP GWLAWQ ++FAQ T
Sbjct: 421 HNSQIRAAADLRPMVGSVKTYLGRPWKKYSRTVIMRSYIDWLVSPAGWLAWQSSKFAQAT 480

Query: 481 LYYGEYRNFGPRASTRLRVRWPGFHAITTRNVASKFTVRRLIAGHTWLPATGVPFKL 533
           LYYGEYRN GPRASTR RV+WPGFH I + NVASKF+V+RLIAG TWLPATGVPFKL
Sbjct: 481 LYYGEYRNIGPRASTRFRVKWPGFHVIKSPNVASKFSVQRLIAGQTWLPATGVPFKL 527

BLAST of CmaCh16G008360 vs. TrEMBL
Match: B9R9R4_RICCO (Pectinesterase OS=Ricinus communis GN=RCOM_1500180 PE=4 SV=1)

HSP 1 Score: 639.8 bits (1649), Expect = 2.9e-180
Identity = 319/526 (60.65%), Postives = 402/526 (76.43%), Query Frame = 1

Query: 7   IAFFSIALFALLSNSSSSPTKTSANTINKWCNTTPHPKVCIFFMSPL--KSPPQNRIEFR 66
           +A F I   +++  ++S  +KT+   +  WC+ TPHP+ C +F+     +   ++R EFR
Sbjct: 8   LAIFFIFFSSIIFTATSISSKTN---VTWWCSQTPHPETCKYFIGHRLQRVALKHRSEFR 67

Query: 67  KMAIKTTLEGAAKAQNYVSRFGPTCKTTRQRAAWTDCFNLYNDTVFQLNRTIRCLHAGTR 126
           K+ ++  LE A  AQ  VS+  P C+   QRA W DC  L++DT+ QLNRT++ L A  +
Sbjct: 68  KITVQVALERALSAQKKVSKLRPKCQDHHQRAVWVDCHKLHSDTIVQLNRTLQGL-AAKK 127

Query: 127 LRHCTDFDAQTWLSSALTNIDMCSSGAADLNVTDFITPIKCQNVSRMISNCLAINGRFLE 186
            + CTDFDAQTWLS+ALTNI  C +G+ D NV+DFI PI   N+S++ISN LAING  L 
Sbjct: 128 KKSCTDFDAQTWLSTALTNIQTCRTGSLDFNVSDFIMPIVSSNLSKLISNGLAINGVLLS 187

Query: 187 GETKSRTTGAEEYQNGFPRWVSSGDRKLLQSARVRAHLVVAKDGSGKFRTVQAAINAAAR 246
            +  S        +  FPRW S  +R+LLQ+  ++A+LVVA+DGSG FRTVQAAINAAA+
Sbjct: 188 VQNNS-------IEGLFPRWFSRKERRLLQAPSIKANLVVAQDGSGHFRTVQAAINAAAK 247

Query: 247 RRGRSRFVIYIKRGVYRENIEIGNDNANIMLVGDGIRFTVITSGRSVAAGFTTFNSATAG 306
           RR  +RFVI++K+GVYRENIE+G +N NI LVGDG+R T+ITSGRSV AG+TT++SATAG
Sbjct: 248 RRYGTRFVIHVKKGVYRENIEVGINNNNIWLVGDGLRNTIITSGRSVGAGYTTYSSATAG 307

Query: 307 IQGPGFMARGIRFVNTAGPRMGQAVALRSSSDLSVFHRCSFEGYQDTLMVLSQRQFYKQC 366
           I G  F+ARGI F NTAGP  GQAVALRS+SDLSVF+RCSFEGYQDTL V SQRQFY++C
Sbjct: 308 IDGLRFVARGITFRNTAGPLKGQAVALRSASDLSVFYRCSFEGYQDTLFVHSQRQFYREC 367

Query: 367 YVYGTIDFIFGNAAVVLQNCLIYVRRPLKGQVNVITAQGREDPFQNSGISIHNSQIRAAA 426
           YVYGTIDFIFGNAAVV QNC+IYVRRPLKGQ N+ITAQGR DPFQN+GISIHNS+I  A 
Sbjct: 368 YVYGTIDFIFGNAAVVFQNCIIYVRRPLKGQANMITAQGRNDPFQNTGISIHNSRILPAP 427

Query: 427 DLQPMIGSVKTYLGRPWKKYSRTVIMRSYIDSLVSPMGWLAWQGTRFAQDTLYYGEYRNF 486
           DL+P++ +V+TYLGRPW +YSRTV+++SYIDS + P GW  WQG+ FA +TLYY EY+NF
Sbjct: 428 DLKPVVRAVQTYLGRPWMQYSRTVVLKSYIDSFIHPAGWSQWQGSNFALNTLYYAEYKNF 487

Query: 487 GPRASTRLRVRWPGFHAITTRNVASKFTVRRLIAGHTWLPATGVPF 531
           GP +STR RV+W G+H IT+  VAS FTV RLIAG +WLPATGVPF
Sbjct: 488 GPGSSTRRRVKWKGYHVITSSTVASSFTVGRLIAGQSWLPATGVPF 522

BLAST of CmaCh16G008360 vs. TrEMBL
Match: A0A061FZE1_THECC (Pectinesterase OS=Theobroma cacao GN=TCM_014943 PE=4 SV=1)

HSP 1 Score: 628.6 bits (1620), Expect = 6.8e-177
Identity = 310/534 (58.05%), Postives = 400/534 (74.91%), Query Frame = 1

Query: 5   IQIAFFSIALFALLSNSSSSPTKTSANTINKWCNTTPHPKVCIFFM--SPLKSPPQNRIE 64
           +++    I L +  S  S++  + S + I  WC+ TPHP+ C +FM     +  P++  E
Sbjct: 1   MEVKILLIFLISFSSIFSTALARGSRSNITWWCSKTPHPEPCKYFMRHGSHRFAPEHMTE 60

Query: 65  FRKMAIKTTLEGAAKAQNYVSRFGPTCKTTRQRAAWTDCFNLYNDTVFQLNRTIRCLHAG 124
           FRKM ++  L+ A  A+  V  FG +C++  Q+AAW DC  L+++T+ QLN T++ L  G
Sbjct: 61  FRKMIVRVALDRAVVAEKNVLEFGQSCESRHQKAAWRDCLKLFDNTILQLNLTLQGLETG 120

Query: 125 TRLRHCTDFDAQTWLSSALTNIDMCSSGAADLNVTDFITPIKCQNVSRMISNCLAINGRF 184
            R+  C+DFDAQTWLS+ALTNI  C +G  D NV+DF TPI   N+S++ISN LA+NG F
Sbjct: 121 RRIS-CSDFDAQTWLSTALTNIQTCEAGFMDFNVSDFSTPITSNNISQLISNSLAVNGVF 180

Query: 185 LEGETKSRTTGAEEYQNGFPRWVSSGDRKLLQSA--RVRAHLVVAKDGSGKFRTVQAAIN 244
           L  +  +  +         P W+S  DR LL SA  +V+AHLVVAKDGSG F+TVQAA++
Sbjct: 181 LHMKNHTEVS---------PGWLSRHDRWLLASASTKVKAHLVVAKDGSGNFKTVQAALD 240

Query: 245 AAARRRGRSRFVIYIKRGVYRENIEIGNDNANIMLVGDGIRFTVITSGRSVAAGFTTFNS 304
           AAA+RR  SR +IY+K+GVYRENIE+ N N+NIML+GDG++ T+ITS RSV  G+TT++S
Sbjct: 241 AAAKRRRTSRLIIYVKKGVYRENIEVVNTNSNIMLIGDGMKRTIITSSRSVKTGYTTYSS 300

Query: 305 ATAGIQGPGFMARGIRFVNTAGPRMGQAVALRSSSDLSVFHRCSFEGYQDTLMVLSQRQF 364
           ATAGI GPGF+AR I F NTAGP  GQAVALRS+SDLSVF+RC+ +GYQDTLMV SQRQF
Sbjct: 301 ATAGIDGPGFIARDITFSNTAGPTKGQAVALRSASDLSVFYRCAIQGYQDTLMVHSQRQF 360

Query: 365 YKQCYVYGTIDFIFGNAAVVLQNCLIYVRRPLKGQVNVITAQGREDPFQNSGISIHNSQI 424
           Y++CY+YGTID IFGNAAVVLQNC I+ R+PLKGQ N+ITAQGR DP+QN+GI+IHNS+I
Sbjct: 361 YRECYIYGTIDIIFGNAAVVLQNCSIFARKPLKGQANIITAQGRGDPYQNTGIAIHNSRI 420

Query: 425 RAAADLQPMIGSVKTYLGRPWKKYSRTVIMRSYIDSLVSPMGWLAWQGTRFAQDTLYYGE 484
            AA DL+P++ S KTYLGRPW+KYSRTV++++Y+ SLVSP GW  W  + FA DTLYYGE
Sbjct: 421 LAANDLKPVVRSFKTYLGRPWQKYSRTVVLKTYLGSLVSPAGWSKWGDSNFALDTLYYGE 480

Query: 485 YRNFGPRASTRLRVRWPGFHAITTRNVASKFTVRRLIAGHTWLPATGVPFKLDL 535
           Y+N GP +STR RV+W GFH IT+ NVAS+FTV  LIAG +WLPATGVPF   L
Sbjct: 481 YKNNGPASSTRYRVKWRGFHVITSPNVASRFTVNSLIAGGSWLPATGVPFTAGL 524

BLAST of CmaCh16G008360 vs. TrEMBL
Match: V4SD84_9ROSI (Pectinesterase OS=Citrus clementina GN=CICLE_v10004719mg PE=4 SV=1)

HSP 1 Score: 628.6 bits (1620), Expect = 6.8e-177
Identity = 317/536 (59.14%), Postives = 405/536 (75.56%), Query Frame = 1

Query: 4   HIQIAFFSIALFALLSNSSSSPTKTSANTINKWCNTTPHPKVCIFFMSPL--KSPPQNRI 63
           +I +    I+L ++  ++ S   + SA  I  WC+ TPHP+ C +F+S    +   ++R 
Sbjct: 5   NISLLIIFISLSSIFYHALSR--RPSATNITWWCSKTPHPEPCNYFLSRSHHRYTFKHRS 64

Query: 64  EFRKMAIKTTLEGAAKAQNYVSRFGPTCKTTRQRAAWTDCFNLYNDTVFQLNRTIRCLHA 123
           +FRK+ ++  +E A KA + V  FG  C+   Q+AAW+DC  LY +TV QLNRT++ L +
Sbjct: 65  DFRKIIVQVAIEQAHKAHDQVMEFGTNCENKHQKAAWSDCVKLYANTVLQLNRTLQGLKS 124

Query: 124 GTRLRHCTDFDAQTWLSSALTNIDMCSSGAADLNVTDFITPIKCQNVSRMISNCLAINGR 183
               R CTDFDAQTWLS+ALTNI  C SG  +LNV+DFITP+   N+S++ISN LA+NG 
Sbjct: 125 D---RSCTDFDAQTWLSTALTNIRTCQSGYMELNVSDFITPVMSNNLSQLISNSLAVNGV 184

Query: 184 FLEGETKSRTTGAEEYQNGFPRWVSSGDRKLLQSARV--RAHLVVAKDGSGKFRTVQAAI 243
            L+ E  +       Y NGFP W+S  +R LL+S+ +  RA+LVVA DGSG +RT+QAAI
Sbjct: 185 LLKRENVT-------YTNGFPSWLSGHERNLLESSSLEARANLVVATDGSGNYRTIQAAI 244

Query: 244 NAAARRRGRSRFVIYIKRGVYRENIEIGNDNANIMLVGDGIRFTVITSGRSVAAGFTTFN 303
           NAAARRRG  RF+I++KRGVYRENIE+G +N NIMLVG+G+R T+ITSG+SV +G TT++
Sbjct: 245 NAAARRRGSGRFIIHVKRGVYRENIEVGLNNNNIMLVGEGMRNTIITSGQSVGSGSTTYS 304

Query: 304 SATAGIQGPGFMARGIRFVNTAGPRMGQAVALRSSSDLSVFHRCSFEGYQDTLMVLSQRQ 363
           SATAGI G  FM R I F NTAGP  GQAVALRS+SDLSVF+RC+F+GYQDTLMV SQRQ
Sbjct: 305 SATAGIDGLHFMGRDITFQNTAGPLKGQAVALRSASDLSVFYRCAFQGYQDTLMVHSQRQ 364

Query: 364 FYKQCYVYGTIDFIFGNAAVVLQNCLIYVRRPLKGQVNVITAQGREDPFQNSGISIHNSQ 423
           FYK+CY+YGTIDFIFGNAAVV QNC+I+VR+PLKGQ NVITAQGR DPFQN+ ISIH+S+
Sbjct: 365 FYKKCYIYGTIDFIFGNAAVVFQNCIIFVRKPLKGQANVITAQGRNDPFQNTAISIHSSR 424

Query: 424 IRAAADLQPMIGSVKTYLGRPWKKYSRTVIMRSYIDSLVSPMGWLAWQ-GTRFAQDTLYY 483
           +  A DL+P++ + KTYLGRPW++YSRTVI+++YID  VSP+GW  W  G  FA DTL+Y
Sbjct: 425 VLPANDLKPVVRNFKTYLGRPWQQYSRTVILKTYIDGFVSPLGWSTWSPGNNFALDTLFY 484

Query: 484 GEYRNFGPRASTRLRVRWPGFHAITTRNVASKFTVRRLIAGHTWLPATGVPFKLDL 535
           GEY N+GP +STR RV+W GFH IT+  VAS+FTV  LIAG +WLPATGVPF L L
Sbjct: 485 GEYENYGPGSSTRHRVKWRGFHVITSPKVASQFTVGSLIAGRSWLPATGVPFILGL 528

BLAST of CmaCh16G008360 vs. TrEMBL
Match: A0A162A9F0_DAUCA (Uncharacterized protein OS=Daucus carota subsp. sativus GN=DCAR_014626 PE=4 SV=1)

HSP 1 Score: 625.5 bits (1612), Expect = 5.7e-176
Identity = 314/524 (59.92%), Postives = 390/524 (74.43%), Query Frame = 1

Query: 11  SIALFALLSNSSSSPTKTSANTINKWCNTTPHPKVCIFFMS-PLKSPPQNRIEFRKMAIK 70
           S ++F  L +S  SPT  +   I+ WC TTPHP+ C +F+       PQNR  FR++  +
Sbjct: 15  SFSIFQALPDSDESPT--AGGDISFWCQTTPHPEPCKYFLGHQFDIRPQNRTHFRQLMFQ 74

Query: 71  TTLEGAAKAQNYVSRFGPTCKTTRQRAAWTDCFNLYNDTVFQLNRTIRCLHAGTRLRHCT 130
            TL+ A  AQNY +     C++ R+RAAW DC  LY DT+FQLNRT+          +C+
Sbjct: 75  VTLDRAQHAQNYTAALESQCRSRRKRAAWNDCNKLYRDTIFQLNRTLEGTKTNATGLNCS 134

Query: 131 DFDAQTWLSSALTNIDMCSSGAADLNVTDFITPIKCQNVSRMISNCLAINGRFLEGETKS 190
            FDAQTWLS+ALTN++ C SG+ +LNV+ FI PI   NVS MISN LA+N  FL     +
Sbjct: 135 SFDAQTWLSAALTNLETCRSGSVELNVSKFIAPIIGNNVSEMISNSLAVNEGFLTPRNDT 194

Query: 191 RTTGAEEYQNGFPRWVSSGDRKLLQSAR--VRAHLVVAKDGSGKFRTVQAAIN-AAARRR 250
                E     FP W +SGDR+LLQS+    RA++VVAKD SG+F +VQAAIN AA  +R
Sbjct: 195 NNGDQE-----FPSWFTSGDRRLLQSSSWSSRANVVVAKDRSGRFSSVQAAINYAATAKR 254

Query: 251 GRSRFVIYIKRGVYRENIEIGNDNANIMLVGDGIRFTVITSGRSVAAGFTTFNSATAGIQ 310
           G +RF+IY+KRGVY ENI + N+  NIMLVGDG+R+T+IT  RSVA GFTT++SATAGI 
Sbjct: 255 GNARFIIYVKRGVYAENIAVANNLNNIMLVGDGLRYTIITGSRSVARGFTTYSSATAGID 314

Query: 311 GPGFMARGIRFVNTAGPRMGQAVALRSSSDLSVFHRCSFEGYQDTLMVLSQRQFYKQCYV 370
           G GF+ARGI F NTAGP+ GQAVALRS+SDLSV++ C FEGYQDTL+V +QRQFYK CY+
Sbjct: 315 GSGFIARGITFRNTAGPQNGQAVALRSASDLSVYYACGFEGYQDTLLVHAQRQFYKLCYI 374

Query: 371 YGTIDFIFGNAAVVLQNCLIYVRRPLKGQVNVITAQGREDPFQNSGISIHNSQIRAAADL 430
           YGTIDFIFGNAAVV QNCLIYVRRPL GQVNVITAQGR DP+QN+GISI  S+I AA DL
Sbjct: 375 YGTIDFIFGNAAVVFQNCLIYVRRPLHGQVNVITAQGRADPYQNTGISIQFSRIMAAPDL 434

Query: 431 QPMIGSVKTYLGRPWKKYSRTVIMRSYIDSLVSPMGWLAWQGTRFAQDTLYYGEYRNFGP 490
            P++ + +TYLGRPW++YSRTV++++YIDSL+SP GWLAWQ + FA DTLYYGEYRN GP
Sbjct: 435 VPVLQTTRTYLGRPWQQYSRTVLIKTYIDSLISPQGWLAWQNSNFAWDTLYYGEYRNIGP 494

Query: 491 RASTRLRVRWPGFHAITTRNVASKFTVRRLIAGHTWLPATGVPF 531
            +STR RV+W G+  I++ N AS+FTV  LIAG TWL +TGVPF
Sbjct: 495 GSSTRNRVKWKGYRVISSANEASRFTVANLIAGRTWLGSTGVPF 531

BLAST of CmaCh16G008360 vs. TAIR10
Match: AT3G47400.1 (AT3G47400.1 Plant invertase/pectin methylesterase inhibitor superfamily)

HSP 1 Score: 598.2 bits (1541), Expect = 5.0e-171
Identity = 298/511 (58.32%), Postives = 383/511 (74.95%), Query Frame = 1

Query: 36  WCNTTPHPKVCIFFMSPLKS------PPQNRIEFRKMAIKTTLEGAAKAQNYVSRFGPTC 95
           WCN TPH + C ++            PP+ R EF +M +K  L+ A    +   +FGP+C
Sbjct: 87  WCNKTPHAETCNYYFRKSSQNNINLRPPRFRSEFLRMLVKVALDQAVITHSQTVKFGPSC 146

Query: 96  KTTRQRAAWTDCFNLYNDTVFQLNRTIRCLH-AGTRLRHCTDFDAQTWLSSALTNIDMCS 155
              +++AAW+DC NL+ +TV QLNRT++ L+ A +    CTDFDAQTWLS+A TNI+ C 
Sbjct: 147 TNNQRKAAWSDCVNLFQNTVAQLNRTLKGLNPAASSDVKCTDFDAQTWLSTAQTNIETCR 206

Query: 156 SGAADLNVTDFITP-IKCQNVSRMISNCLAINGRFLEGETKSRTTGAEEYQNGFPRWVSS 215
           SG+ DLNV+DF+ P I  +N+S +I NCLA+NG  ++    + T   +EY   FP WVS 
Sbjct: 207 SGSEDLNVSDFVMPVISNKNLSDLIGNCLAVNGVLMKQHDHTTTANHKEY---FPSWVSR 266

Query: 216 GDRKLLQSARVRA---HLVVAKDGSGKFRTVQAAINAAARRRGRSRFVIYIKRGVYRENI 275
            +R+LL SA +     HLVVA+D SG FR++QAAIN AARRR +SRFVIY+K+GVYRENI
Sbjct: 267 HERRLLVSASLAKSSPHLVVAQDRSGHFRSIQAAINFAARRRFKSRFVIYVKKGVYRENI 326

Query: 276 EIGNDNANIMLVGDGIRFTVITSGRSVAAGFTTFNSATAGIQGPGFMARGIRFVNTAGPR 335
           ++GNDN NIMLVGDG R T+ITSGRSV  G+TT+NSAT G  G  F+A+ + F+NTAGP 
Sbjct: 327 DVGNDNHNIMLVGDGERKTIITSGRSVQHGYTTYNSATGGFGGQRFVAKDMTFINTAGPL 386

Query: 336 MGQAVALRSSSDLSVFHRCSFEGYQDTLMVLSQRQFYKQCYVYGTIDFIFGNAAVVLQNC 395
            GQAVA+RSSSDLSVF+R    G+QDTL + SQRQF+++CY+ GTIDFIFGNAAVV QNC
Sbjct: 387 RGQAVAVRSSSDLSVFYRVGIHGFQDTLYIHSQRQFFRECYISGTIDFIFGNAAVVFQNC 446

Query: 396 LIYVRRPLKGQVNVITAQGREDPFQNSGISIHNSQIRAAADLQPMIGSVKTYLGRPWKKY 455
           +I VRRPL GQ N+ITAQGR DPFQN+GI+IH+S+I AA+DL+P+I + KTYLGRPW+ Y
Sbjct: 447 MILVRRPLHGQANIITAQGRGDPFQNTGITIHSSRIIAASDLKPVIRAYKTYLGRPWQAY 506

Query: 456 SRTVIMRSYIDSLVSPMGWLAW-QGTRFAQDTLYYGEYRNFGPRASTRLRVRWPGFHAIT 515
           SR  IM++YID+ +SP+GW  W +G+ FA +T++YGEY+NFGP +STR RVRW GFHAIT
Sbjct: 507 SRVTIMKTYIDNSISPLGWSPWLRGSNFALNTVFYGEYKNFGPGSSTRWRVRWKGFHAIT 566

Query: 516 TRNVASKFTVRRLIAGHTWLPATGVPFKLDL 535
           +  VAS+FTV  LIAG +WLPATGVPFK  L
Sbjct: 567 STAVASRFTVGSLIAGGSWLPATGVPFKSGL 594

BLAST of CmaCh16G008360 vs. TAIR10
Match: AT5G51490.1 (AT5G51490.1 Plant invertase/pectin methylesterase inhibitor superfamily)

HSP 1 Score: 577.0 bits (1486), Expect = 1.2e-164
Identity = 299/538 (55.58%), Postives = 381/538 (70.82%), Query Frame = 1

Query: 1   MAPHIQIAFFSIALFALLSNSSSSPTKTSAN-TINKWCNTTPHPKVCIFFMSPLKS--PP 60
           M   + I F  + L  LL      P  T A+     WC+ TP+P  C  +         P
Sbjct: 4   MMQKLSILFLHLILLVLLC---VHPLTTVADRNSTDWCDKTPYPDPCKCYFKNHNGFQQP 63

Query: 61  QNRIEFRKMAIKTTLEGAAKAQNYVSRFGPTCKTTRQRAAWTDCFNLYNDTVFQLNRTIR 120
               EFR M ++  ++ A  A+  ++  G  C  ++++A   DC +LY DT+ QLNRT+ 
Sbjct: 64  TQLSEFRVMLVEAAMDRAISARAELTNSGKNCTDSKKQAVLADCIDLYGDTIMQLNRTLH 123

Query: 121 CLH--AGTRLRHCTDFDAQTWLSSALTNIDMCSSGAADLNVTDFITPIKCQ-NVSRMISN 180
            +   AG   + CTDFDAQTWLS+ALTN + C  G++DLNVTDFITPI     +S +ISN
Sbjct: 124 GVSPKAGAA-KSCTDFDAQTWLSTALTNTETCRRGSSDLNVTDFITPIVSNTKISHLISN 183

Query: 181 CLAINGRFLEGETKSRTTGAEEYQNGFPRWVSSGDRKLLQSARVRAHLVVAKDGSGKFRT 240
           CLA+NG  L    K  TT     Q GFP W+S  D++LL++  VRA+LVVAKDGSG F T
Sbjct: 184 CLAVNGALLTAGNKGNTTA---NQKGFPTWLSRKDKRLLRA--VRANLVVAKDGSGHFNT 243

Query: 241 VQAAINAAARRRGRS-RFVIYIKRGVYRENIEIGNDNANIMLVGDGIRFTVITSGRSVAA 300
           VQAAI+ A RR+  S RFVIY+KRG+Y+ENI +  +N +IMLVGDG+R T+IT GRSV  
Sbjct: 244 VQAAIDVAGRRKVTSGRFVIYVKRGIYQENINVRLNNDDIMLVGDGMRSTIITGGRSVQG 303

Query: 301 GFTTFNSATAGIQGPGFMARGIRFVNTAGPRMGQAVALRSSSDLSVFHRCSFEGYQDTLM 360
           G+TT+NSATAGI+G  F+A+GI F NTAGP  GQAVALRSSSDLS+F++CS EGYQDTLM
Sbjct: 304 GYTTYNSATAGIEGLHFIAKGITFRNTAGPAKGQAVALRSSSDLSIFYKCSIEGYQDTLM 363

Query: 361 VLSQRQFYKQCYVYGTIDFIFGNAAVVLQNCLIYVRRPLKGQVNVITAQGREDPFQNSGI 420
           V SQRQFY++CY+YGT+DFIFGNAA V QNCLI  RRPLKGQ NVITAQGR DPFQN+GI
Sbjct: 364 VHSQRQFYRECYIYGTVDFIFGNAAAVFQNCLILPRRPLKGQANVITAQGRADPFQNTGI 423

Query: 421 SIHNSQIRAAADLQPMIGSVKTYLGRPWKKYSRTVIMRSYIDSLVSPMGWLAW-QGTRFA 480
           SIHNS+I  A DL+P++G+VKTY+GRPW K+SRTV++++Y+D++VSP+GW  W +G+ F 
Sbjct: 424 SIHNSRILPAPDLKPVVGTVKTYMGRPWMKFSRTVVLQTYLDNVVSPVGWSPWIEGSVFG 483

Query: 481 QDTLYYGEYRNFGPRASTRLRVRWPGFHAITTRNVASKFTVRRLIAGHTWLPATGVPF 531
            DTL+Y EY+N GP +STR RV W GFH +   + AS FTV + IAG  WLP TG+PF
Sbjct: 484 LDTLFYAEYKNTGPASSTRWRVSWKGFHVLGRASDASAFTVGKFIAGTAWLPRTGIPF 532

BLAST of CmaCh16G008360 vs. TAIR10
Match: AT5G51500.1 (AT5G51500.1 Plant invertase/pectin methylesterase inhibitor superfamily)

HSP 1 Score: 576.2 bits (1484), Expect = 2.0e-164
Identity = 296/537 (55.12%), Postives = 385/537 (71.69%), Query Frame = 1

Query: 7   IAFFSIALFALLS---NSSSSPTKTSANTINKWCNTTPHPKVCIFFM---SPLKSPPQNR 66
           I+F S+ L  +L       ++    ++  I+ WC+ TP+P  C  +    S  + P Q  
Sbjct: 9   ISFLSLHLLLILLLCLRPLTTVADGNSTNIDGWCDKTPYPYPCKRYFIKHSGFRLPTQIS 68

Query: 67  IEFRKMAIKTTLEGAAKAQNYVSRFGPTCKTTRQRAAWTDCFNLYNDTVFQLNRTIRCLH 126
            EFR + ++  ++ A  A + ++     C   +++A   DC NLY DTV QLNRT++ + 
Sbjct: 69  -EFRVLLVEAAMDRAVSAWDKLTNSSKNCTDFKKQAVLADCINLYGDTVMQLNRTLQGVS 128

Query: 127 AGTRLRHCTDFDAQTWLSSALTNIDMCSSGAADLNVTDFITPIKCQN-VSRMISNCLAIN 186
           + T  R CTDFDAQTWLS+ALTN + C  G++DLNV+DF TPI     +S +ISNCLA+N
Sbjct: 129 SKTG-RRCTDFDAQTWLSTALTNTETCRRGSSDLNVSDFTTPIVSNTKISHLISNCLAVN 188

Query: 187 GRFLEGETKSRTTGAEEYQNGFPRWVSSGDRKLLQSARVRAHLVVAKDGSGKFRTVQAAI 246
           G  L       TTG  +   GFP WVS  +R+LLQ   VRA+LVVAKDGSG F+TVQAAI
Sbjct: 189 GALLTAGKNDSTTGDSK---GFPTWVSRKERRLLQLQSVRANLVVAKDGSGHFKTVQAAI 248

Query: 247 NAAARRRGRS-RFVIYIKRGVYRENIEIGNDNANIMLVGDGIRFTVITSGRSVAAGFTTF 306
           + A RR+  S RFVIY+KRG+Y+EN+ +  +N NIMLVGDG+R+T+IT GRSV  G+TT+
Sbjct: 249 DVAGRRKVTSGRFVIYVKRGIYQENLNVRLNNDNIMLVGDGMRYTIITGGRSVKGGYTTY 308

Query: 307 NSATAGIQGPGFMARGIRFVNTAGPRMGQAVALRSSSDLSVFHRCSFEGYQDTLMVLSQR 366
           +SATAGI+G  F+A+GI F NTAGP  GQAVALRSSSDLS+F+RCS EGYQDTLMV SQR
Sbjct: 309 SSATAGIEGLHFIAKGIAFQNTAGPAKGQAVALRSSSDLSIFYRCSIEGYQDTLMVHSQR 368

Query: 367 QFYKQCYVYGTIDFIFGNAAVVLQNCLIYVRRPLKGQVNVITAQGREDPFQNSGISIHNS 426
           QFY++CY+YGT+DFIFGNAAVV QNC+I  R PLKGQ NVITAQGR D FQN+GISIHNS
Sbjct: 369 QFYRECYIYGTVDFIFGNAAVVFQNCIILPRLPLKGQANVITAQGRTDLFQNTGISIHNS 428

Query: 427 QIRAAADLQPMIGSVKTYLGRPWKKYSRTVIMRSYIDSLVSPMGWLAW-QGTRFAQDTLY 486
            I  A DL+P++ SVKTY+GRPW  YSRTV++++YIDS+VSP+GW  W +G+ +  DTL+
Sbjct: 429 IIIPAPDLKPVVRSVKTYMGRPWMMYSRTVVLKTYIDSVVSPVGWSPWTKGSTYGLDTLF 488

Query: 487 YGEYRNFGPRASTRLRVRWPGFHAITTRNVASKFTVRRLIAGHTWLPATGVPFKLDL 535
           Y EY+N GP +STR RVRW GFH ++  + AS F+V + IAG  WLP +G+PF  +L
Sbjct: 489 YAEYKNIGPASSTRWRVRWKGFHVLSKASDASAFSVGKFIAGTAWLPGSGIPFTSEL 540

BLAST of CmaCh16G008360 vs. TAIR10
Match: AT2G45220.1 (AT2G45220.1 Plant invertase/pectin methylesterase inhibitor superfamily)

HSP 1 Score: 504.2 bits (1297), Expect = 9.8e-143
Identity = 262/500 (52.40%), Postives = 343/500 (68.60%), Query Frame = 1

Query: 33  INKWCNTTPHPKVCIFFMSPLKS--PPQNRIEFRKMAIKTTLEGAAKAQNYVSRFGPTCK 92
           +  WC+ TP+PK C +F++   +  P ++  EF K+++K  L+ A  A+ +    GP C+
Sbjct: 30  VKAWCSQTPNPKPCEYFLTHNSNNEPIKSESEFLKISMKLVLDRAILAKTHAFTLGPKCR 89

Query: 93  TTRQRAAWTDCFNLYNDTVFQLNRTIRCLHAGTRLRHCTDFDAQTWLSSALTNIDMCSSG 152
            TR++AAW DC  LY+ TV ++N T+        ++ C+  DAQTWLS+ALTN+D C +G
Sbjct: 90  DTREKAAWEDCIKLYDLTVSKINETM-----DPNVK-CSKLDAQTWLSTALTNLDTCRAG 149

Query: 153 AADLNVTDFITPIKCQNVSRMISNCLAINGRFLEGETKSRTTGAEEYQNGFPRWVSSGDR 212
             +L VTD + P+   NVS ++ N LAIN        K         ++GFP WV  GDR
Sbjct: 150 FLELGVTDIVLPLMSNNVSNLLCNTLAIN--------KVPFNYTPPEKDGFPSWVKPGDR 209

Query: 213 KLLQSARVRAHLVVAKDGSGKFRTVQAAINAAARRRGRSRFVIYIKRGVYRENIEIGNDN 272
           KLLQS+  + + VVAKDGSG F+T++ AI+AA+   G  RFVIY+K+GVY EN+EI    
Sbjct: 210 KLLQSSTPKDNAVVAKDGSGNFKTIKEAIDAAS---GSGRFVIYVKQGVYSENLEI--RK 269

Query: 273 ANIMLVGDGIRFTVITSGRSVAAGFTTFNSATAGIQGPGFMARGIRFVNTAGPRMGQAVA 332
            N+ML GDGI  T+IT  +SV  G TTFNSAT    G GF+ARGI F NTAG    QAVA
Sbjct: 270 KNVMLRGDGIGKTIITGSKSVGGGTTTFNSATVAAVGDGFIARGITFRNTAGASNEQAVA 329

Query: 333 LRSSSDLSVFHRCSFEGYQDTLMVLSQRQFYKQCYVYGTIDFIFGNAAVVLQNCLIYVRR 392
           LRS SDLSVF++CSFE YQDTL V S RQFY+ C VYGT+DFIFGNAA VLQNC I+ RR
Sbjct: 330 LRSGSDLSVFYQCSFEAYQDTLYVHSNRQFYRDCDVYGTVDFIFGNAAAVLQNCNIFARR 389

Query: 393 PLKGQVNVITAQGREDPFQNSGISIHNSQIRAAADLQPMIGSVKTYLGRPWKKYSRTVIM 452
           P + + N ITAQGR DP QN+GI IHNS++ AA+DL+P++GS KTYLGRPW++YSRTV M
Sbjct: 390 P-RSKTNTITAQGRSDPNQNTGIIIHNSRVTAASDLRPVLGSTKTYLGRPWRQYSRTVFM 449

Query: 453 RSYIDSLVSPMGWLAWQGTRFAQDTLYYGEYRNFGPRASTRLRVRWPGFHAITTRNVASK 512
           ++ +DSL+ P GWL W G  FA  TL+Y E++N GP AST  RV WPGF  + + + ASK
Sbjct: 450 KTSLDSLIDPRGWLEWDG-NFALKTLFYAEFQNTGPGASTSGRVTWPGFRVLGSASEASK 507

Query: 513 FTVRRLIAGHTWLPATGVPF 531
           FTV   +AG +W+P++ VPF
Sbjct: 510 FTVGTFLAGGSWIPSS-VPF 507

BLAST of CmaCh16G008360 vs. TAIR10
Match: AT4G00190.1 (AT4G00190.1 pectin methylesterase 38)

HSP 1 Score: 402.1 bits (1032), Expect = 5.2e-112
Identity = 220/502 (43.82%), Postives = 309/502 (61.55%), Query Frame = 1

Query: 34  NKWCNTTPHPKVCIFFMSPLKSPPQNRIEFRKMAIKTTLEGAAKAQNYVSRFGPTCKTTR 93
           N+ C+ TPHP  C   +   K P ++  +F +++++ TL+GA KA++      P   +  
Sbjct: 5   NEMCDETPHPGECKTLLIKHK-PIRSTTQFLQVSVERTLDGAVKAKSDTYFLEPQFGS-- 64

Query: 94  QRAAWTDCFNLYNDTVFQLNRTIRCLHAGTRLRHCTDFDAQTWLSSALTNIDMCSSGAAD 153
            + AW +C +LY  T+ +LN ++ C         C+  D Q WLS+ALTN+D C    ++
Sbjct: 65  -KQAWEECMDLYEQTIHRLNESVLCPK-----NVCSRSDVQAWLSTALTNLDTCQEEMSE 124

Query: 154 LNVTDFITPIKCQNVSRMISNCLAINGRFLEGETKSRTTGAEEYQNGFPRWVSSGDRKLL 213
           L V+        ++++  + N LAIN R                QNG    +S    K L
Sbjct: 125 LGVSSH----SLESITIDVINTLAINKRM--------------EQNGKEFGISKITMKTL 184

Query: 214 QSARVRAHLVVAKDGSGKFRTVQAAINAAARR-RGRSRFVIYIKRGVYRENIEIGNDNAN 273
            S   +  +VVA+DGSG ++T+Q A+N A  R +G  R+VI++K+GVY E + +G  + N
Sbjct: 185 -SIGEKVDVVVAQDGSGDYKTIQEAVNGAGERLKGSPRYVIHVKQGVYEEYVNVGIKSNN 244

Query: 274 IMLVGDGIRFTVITSGRSVAAGFTTFNSATAGIQGPGFMARGIRFVNTAGPRMGQAVALR 333
           IM+ GDGI  T+IT  +S   GF+T+ SAT   +G GF+ R I   NTAGP   QAVALR
Sbjct: 245 IMITGDGIGKTIITGDKSKGRGFSTYKSATFVAEGDGFVGRDITIRNTAGPENHQAVALR 304

Query: 334 SSSDLSVFHRCSFEGYQDTLMVLSQRQFYKQCYVYGTIDFIFGNAAVVLQNCLIYVRRPL 393
           S+SD+SVF+RCS EGYQDTL V S RQF+++C +YGT+DFIFGNAA VLQNC I+ R P 
Sbjct: 305 SNSDMSVFYRCSIEGYQDTLYVHSGRQFFRECDIYGTVDFIFGNAAAVLQNCRIFARNPP 364

Query: 394 KGQVNVITAQGREDPFQNSGISIHNSQIRAAADLQPMIGSVKTYLGRPWKKYSRTVIMRS 453
            G VN ITAQ R +P Q +GI IHNS ++ A  +Q  +G VKTYLGRPW+ Y+RTV++ +
Sbjct: 365 NG-VNTITAQSRFNPNQTTGIVIHNSVVKGAPGVQ--LGGVKTYLGRPWRSYARTVVIGT 424

Query: 454 YIDSLVSPMGWLAWQGTRFAQDTLYYGEYRNFGPRASTRLRVRWPGFHAITTRNVASKFT 513
           Y+D+L+ P GW+ W     A  TLYYGEY+N GP + T  RV W GFH I+    A +FT
Sbjct: 425 YLDTLIEPNGWIDWDNVT-ALSTLYYGEYQNSGPGSGTENRVDWAGFHVISDIQEAREFT 474

Query: 514 VRRLIAGHTWLPATGVPFKLDL 535
           + + I   +WLP T VPF ++L
Sbjct: 485 LPKFIDSASWLPPTKVPFTINL 474

BLAST of CmaCh16G008360 vs. NCBI nr
Match: gi|778676190|ref|XP_011650542.1| (PREDICTED: probable pectinesterase/pectinesterase inhibitor 59 [Cucumis sativus])

HSP 1 Score: 852.8 bits (2202), Expect = 3.2e-244
Identity = 429/537 (79.89%), Postives = 466/537 (86.78%), Query Frame = 1

Query: 1   MAPHIQIAFFSIALFALLSNSSSSPTKTSANTINKWCNTTPHPKVCIFFMSPLKSPPQNR 60
           MA H +I F SI  F LLSN+       SAN I KWC TTPHP+VC+  MSP+K PPQNR
Sbjct: 1   MASHYKILFISITFFTLLSNN------VSANPIIKWCKTTPHPRVCVSLMSPIKPPPQNR 60

Query: 61  IEFRKMAIKTTLEGAAKAQNYVSRFGPTCKTTRQRAAWTDCFNLYNDTVFQLNRTIRCLH 120
            EFR+MAI+TTLE AA+A+ Y +RFGPTCKT+RQR AWTDCF LYND V QLNRT+ C+ 
Sbjct: 61  REFRQMAIQTTLEKAAEARAYTARFGPTCKTSRQRTAWTDCFKLYNDVVLQLNRTLHCVV 120

Query: 121 AGTRL--RHCTDFDAQTWLSSALTNIDMCSSGAADLNVTDFITPIKCQNVSRMISNCLAI 180
               +  R CTDFDAQTWLSSALT+ID+C+SGAADLNVTDFITPIKC NVS+MISNCLAI
Sbjct: 121 TDEAIHRRSCTDFDAQTWLSSALTDIDLCNSGAADLNVTDFITPIKCLNVSKMISNCLAI 180

Query: 181 NGRFLEGETKSRTTGAEEYQNG-FPRWVSSGDRKLLQS--ARVRAHLVVAKDGSGKFRTV 240
           NG FLE E      G    +NG FP WVS GDRKLL+S   RVRA+LVVAKDGSG FR V
Sbjct: 181 NGGFLEEEGVKYDDG----RNGSFPMWVSEGDRKLLESRPGRVRANLVVAKDGSGTFRRV 240

Query: 241 QAAINAAARRRGRSRFVIYIKRGVYRENIEIGNDNANIMLVGDGIRFTVITSGRSVAAGF 300
           QAAI+AAARRRGR RF+IY+KRGVYRENIE+GNDN NIMLVGDG+RFTVITSGRSVAAGF
Sbjct: 241 QAAIDAAARRRGRGRFIIYVKRGVYRENIEVGNDNGNIMLVGDGMRFTVITSGRSVAAGF 300

Query: 301 TTFNSATAGIQGPGFMARGIRFVNTAGPRMGQAVALRSSSDLSVFHRCSFEGYQDTLMVL 360
           TTF+SATAGIQGPGF+AR IRFVNTAGPRMGQAVALRSSSDLSVFHRCSFEGYQDTLMVL
Sbjct: 301 TTFSSATAGIQGPGFIARDIRFVNTAGPRMGQAVALRSSSDLSVFHRCSFEGYQDTLMVL 360

Query: 361 SQRQFYKQCYVYGTIDFIFGNAAVVLQNCLIYVRRPLKGQVNVITAQGREDPFQNSGISI 420
           SQRQFYKQCYVYGTIDFIFGNAAVVLQNC+IYVRRPLKGQVNVITAQGREDPFQNSGISI
Sbjct: 361 SQRQFYKQCYVYGTIDFIFGNAAVVLQNCMIYVRRPLKGQVNVITAQGREDPFQNSGISI 420

Query: 421 HNSQIRAAADLQPMIGSVKTYLGRPWKKYSRTVIMRSYIDSLVSPMGWLAWQGTRFAQDT 480
           HNSQIRAAADL+PM+GSVKTYLGRPWKKYSRTVIMRSYID LVSP GWLAWQ ++FAQ T
Sbjct: 421 HNSQIRAAADLRPMVGSVKTYLGRPWKKYSRTVIMRSYIDWLVSPAGWLAWQSSKFAQAT 480

Query: 481 LYYGEYRNFGPRASTRLRVRWPGFHAITTRNVASKFTVRRLIAGHTWLPATGVPFKL 533
           LYYGEYRN GPRASTR RV+WPGFH I + NVASKF+V+RLIAG TWLPATGVPFKL
Sbjct: 481 LYYGEYRNIGPRASTRFRVKWPGFHVIKSPNVASKFSVQRLIAGQTWLPATGVPFKL 527

BLAST of CmaCh16G008360 vs. NCBI nr
Match: gi|659102836|ref|XP_008452338.1| (PREDICTED: probable pectinesterase/pectinesterase inhibitor 59 [Cucumis melo])

HSP 1 Score: 848.6 bits (2191), Expect = 6.0e-243
Identity = 431/539 (79.96%), Postives = 465/539 (86.27%), Query Frame = 1

Query: 1   MAPHIQIAFF-SIAL-FALLSNSSSSPTKTSANTINKWCNTTPHPKVCIFFMSPLKSPPQ 60
           MA H +I  F SI L F +LSN+ S+      N I KWC TTPHPKVCI FMSP+K PPQ
Sbjct: 1   MASHYKIILFISITLLFTILSNNVSAN-----NPIIKWCKTTPHPKVCISFMSPIKPPPQ 60

Query: 61  NRIEFRKMAIKTTLEGAAKAQNYVSRFGPTCKTTRQRAAWTDCFNLYNDTVFQLNRTIRC 120
           NR EFR++AI+TTLE A +A+ Y +RFGPTCKTTRQR AWTDCF LYND VFQLNRT++C
Sbjct: 61  NREEFRQVAIQTTLEKADEAKAYTARFGPTCKTTRQRTAWTDCFKLYNDVVFQLNRTLQC 120

Query: 121 LHAGTRLRH---CTDFDAQTWLSSALTNIDMCSSGAADLNVTDFITPIKCQNVSRMISNC 180
           L     +     CTDFDAQTWLSSALT+ID+C+SGAADLNVTDFI PIKC NVS+MISNC
Sbjct: 121 LVTDETIHRRPTCTDFDAQTWLSSALTDIDLCNSGAADLNVTDFIAPIKCLNVSKMISNC 180

Query: 181 LAINGRFLEGETKSRTTGAEEYQNGFPRWVSSGDRKLLQSA--RVRAHLVVAKDGSGKFR 240
           LAING FLE E      G       FP WVS GDRKLL+S   RVRA+LVVAKDGSGKFR
Sbjct: 181 LAINGGFLEEEGVKYDDGGN---GSFPMWVSEGDRKLLESRPRRVRANLVVAKDGSGKFR 240

Query: 241 TVQAAINAAARRRGRSRFVIYIKRGVYRENIEIGNDNANIMLVGDGIRFTVITSGRSVAA 300
            VQAAI+AAARRRGR RFVIY+KRGVYRENIE+GNDN NIMLVGDG+RFTVITSGRSVAA
Sbjct: 241 RVQAAIDAAARRRGRGRFVIYVKRGVYRENIEVGNDNGNIMLVGDGMRFTVITSGRSVAA 300

Query: 301 GFTTFNSATAGIQGPGFMARGIRFVNTAGPRMGQAVALRSSSDLSVFHRCSFEGYQDTLM 360
           GFTTF+SATAGIQGPGF+AR IRFVNTAGPRMGQAVALRSSSDLSVFHRCSFEGYQDTLM
Sbjct: 301 GFTTFSSATAGIQGPGFIARDIRFVNTAGPRMGQAVALRSSSDLSVFHRCSFEGYQDTLM 360

Query: 361 VLSQRQFYKQCYVYGTIDFIFGNAAVVLQNCLIYVRRPLKGQVNVITAQGREDPFQNSGI 420
           VLSQRQFYKQCY+YGTIDFIFGNAAVVLQNCLIYVRRPLKGQVNVITAQGREDPFQNSGI
Sbjct: 361 VLSQRQFYKQCYIYGTIDFIFGNAAVVLQNCLIYVRRPLKGQVNVITAQGREDPFQNSGI 420

Query: 421 SIHNSQIRAAADLQPMIGSVKTYLGRPWKKYSRTVIMRSYIDSLVSPMGWLAWQGTRFAQ 480
           SIHNSQIRAAADL+PM+GSVKTYLGRPWKKYSRTVIMRSYIDSLVSP GWLAWQ ++FAQ
Sbjct: 421 SIHNSQIRAAADLRPMVGSVKTYLGRPWKKYSRTVIMRSYIDSLVSPAGWLAWQSSKFAQ 480

Query: 481 DTLYYGEYRNFGPRASTRLRVRWPGFHAITTRNVASKFTVRRLIAGHTWLPATGVPFKL 533
            TLYYGEY N GPRASTR RV+WPGFH I + NVASKF+VRRLIAG TWLPATGVPFKL
Sbjct: 481 ATLYYGEYHNIGPRASTRFRVKWPGFHVIKSPNVASKFSVRRLIAGQTWLPATGVPFKL 531

BLAST of CmaCh16G008360 vs. NCBI nr
Match: gi|255539749|ref|XP_002510939.1| (PREDICTED: probable pectinesterase/pectinesterase inhibitor 60 [Ricinus communis])

HSP 1 Score: 639.8 bits (1649), Expect = 4.2e-180
Identity = 319/526 (60.65%), Postives = 402/526 (76.43%), Query Frame = 1

Query: 7   IAFFSIALFALLSNSSSSPTKTSANTINKWCNTTPHPKVCIFFMSPL--KSPPQNRIEFR 66
           +A F I   +++  ++S  +KT+   +  WC+ TPHP+ C +F+     +   ++R EFR
Sbjct: 8   LAIFFIFFSSIIFTATSISSKTN---VTWWCSQTPHPETCKYFIGHRLQRVALKHRSEFR 67

Query: 67  KMAIKTTLEGAAKAQNYVSRFGPTCKTTRQRAAWTDCFNLYNDTVFQLNRTIRCLHAGTR 126
           K+ ++  LE A  AQ  VS+  P C+   QRA W DC  L++DT+ QLNRT++ L A  +
Sbjct: 68  KITVQVALERALSAQKKVSKLRPKCQDHHQRAVWVDCHKLHSDTIVQLNRTLQGL-AAKK 127

Query: 127 LRHCTDFDAQTWLSSALTNIDMCSSGAADLNVTDFITPIKCQNVSRMISNCLAINGRFLE 186
            + CTDFDAQTWLS+ALTNI  C +G+ D NV+DFI PI   N+S++ISN LAING  L 
Sbjct: 128 KKSCTDFDAQTWLSTALTNIQTCRTGSLDFNVSDFIMPIVSSNLSKLISNGLAINGVLLS 187

Query: 187 GETKSRTTGAEEYQNGFPRWVSSGDRKLLQSARVRAHLVVAKDGSGKFRTVQAAINAAAR 246
            +  S        +  FPRW S  +R+LLQ+  ++A+LVVA+DGSG FRTVQAAINAAA+
Sbjct: 188 VQNNS-------IEGLFPRWFSRKERRLLQAPSIKANLVVAQDGSGHFRTVQAAINAAAK 247

Query: 247 RRGRSRFVIYIKRGVYRENIEIGNDNANIMLVGDGIRFTVITSGRSVAAGFTTFNSATAG 306
           RR  +RFVI++K+GVYRENIE+G +N NI LVGDG+R T+ITSGRSV AG+TT++SATAG
Sbjct: 248 RRYGTRFVIHVKKGVYRENIEVGINNNNIWLVGDGLRNTIITSGRSVGAGYTTYSSATAG 307

Query: 307 IQGPGFMARGIRFVNTAGPRMGQAVALRSSSDLSVFHRCSFEGYQDTLMVLSQRQFYKQC 366
           I G  F+ARGI F NTAGP  GQAVALRS+SDLSVF+RCSFEGYQDTL V SQRQFY++C
Sbjct: 308 IDGLRFVARGITFRNTAGPLKGQAVALRSASDLSVFYRCSFEGYQDTLFVHSQRQFYREC 367

Query: 367 YVYGTIDFIFGNAAVVLQNCLIYVRRPLKGQVNVITAQGREDPFQNSGISIHNSQIRAAA 426
           YVYGTIDFIFGNAAVV QNC+IYVRRPLKGQ N+ITAQGR DPFQN+GISIHNS+I  A 
Sbjct: 368 YVYGTIDFIFGNAAVVFQNCIIYVRRPLKGQANMITAQGRNDPFQNTGISIHNSRILPAP 427

Query: 427 DLQPMIGSVKTYLGRPWKKYSRTVIMRSYIDSLVSPMGWLAWQGTRFAQDTLYYGEYRNF 486
           DL+P++ +V+TYLGRPW +YSRTV+++SYIDS + P GW  WQG+ FA +TLYY EY+NF
Sbjct: 428 DLKPVVRAVQTYLGRPWMQYSRTVVLKSYIDSFIHPAGWSQWQGSNFALNTLYYAEYKNF 487

Query: 487 GPRASTRLRVRWPGFHAITTRNVASKFTVRRLIAGHTWLPATGVPF 531
           GP +STR RV+W G+H IT+  VAS FTV RLIAG +WLPATGVPF
Sbjct: 488 GPGSSTRRRVKWKGYHVITSSTVASSFTVGRLIAGQSWLPATGVPF 522

BLAST of CmaCh16G008360 vs. NCBI nr
Match: gi|645257387|ref|XP_008234390.1| (PREDICTED: probable pectinesterase/pectinesterase inhibitor 60 [Prunus mume])

HSP 1 Score: 638.6 bits (1646), Expect = 9.4e-180
Identity = 321/529 (60.68%), Postives = 403/529 (76.18%), Query Frame = 1

Query: 11  SIALFALLSNSSSSPTKTSANTINKWCNTTPHPKVCIFFMSPLKSP--PQNRIEFRKMAI 70
           SI +F+ +  ++ S +   +N I  WC  TPHP+ C + MS  +    P++  EFRKM +
Sbjct: 10  SILIFSFIFQTALSRSHHHSNRIRTWCQKTPHPEPCNYCMSHSRHRLVPKHTSEFRKMMV 69

Query: 71  KTTLEGAAKAQNYVSRFGPTCKTTRQRAAWTDCFNLYNDTVFQLNRTIRCLHAGTRLRHC 130
           +  LE A  A++Y S+FG  C+  +Q+AAW DC  L+ DTV QLN T+  L  GT+ R+C
Sbjct: 70  QVALERALNAKSYASQFGQNCQNNQQKAAWADCLKLFEDTVHQLNITLDGL--GTK-RNC 129

Query: 131 TDFD-AQTWLSSALTNIDMCSSGAADLNVTDFITPIKCQN--VSRMISNCLAINGRFLEG 190
           ++FD AQTWLS+ALTNI  C  G+ +LNV+DFI PI   N  VS +ISN LAI  + L  
Sbjct: 130 SNFDDAQTWLSAALTNIHTCQVGSMELNVSDFIAPIYTSNNNVSELISNGLAIGSQLLGT 189

Query: 191 ETKSRTTGAEEYQNGFPRWVSSGDRKLLQSARVRAHLVVAKDGSGKFRTVQAAINAAARR 250
           E        EEY    P WVS  +R+LLQ+++++A+LVVAKDGSG FRTVQAAI+AAA+R
Sbjct: 190 E---ENYADEEY----PNWVSKHERRLLQASKIKANLVVAKDGSGHFRTVQAAIDAAAKR 249

Query: 251 RGRSRFVIYIKRGVYRENIEIGNDNANIMLVGDGIRFTVITSGRSVAAGFTTFNSATAGI 310
           +  SRF+IY+K+GVYRENIE+ N N NIML+G  +R+T+IT+ RSV  G TT+NSATAGI
Sbjct: 250 KITSRFIIYVKKGVYRENIEVSNTNNNIMLIGASMRYTIITASRSVNGGSTTYNSATAGI 309

Query: 311 QGPGFMARGIRFVNTAGPRMGQAVALRSSSDLSVFHRCSFEGYQDTLMVLSQRQFYKQCY 370
            GPGF+ARGI F NTAGP  GQAVALRS+SDLSVF+RC+F+GYQDTLMV SQRQFY++CY
Sbjct: 310 DGPGFIARGITFSNTAGPLKGQAVALRSASDLSVFYRCAFQGYQDTLMVHSQRQFYRECY 369

Query: 371 VYGTIDFIFGNAAVVLQNCLIYVRRPLKGQVNVITAQGREDPFQNSGISIHNSQIRAAAD 430
           +YGTIDFIFGNAA V Q+C+IYVRRPLKGQVNVITAQ R DP+QN+ ISIHNSQ+RAAAD
Sbjct: 370 IYGTIDFIFGNAAAVFQSCIIYVRRPLKGQVNVITAQARNDPYQNTAISIHNSQVRAAAD 429

Query: 431 LQPMIGSVKTYLGRPWKKYSRTVIMRSYIDSLVSPMGWLAWQGTRFAQDTLYYGEYRNFG 490
           L+P++ + KTYLGRPW +YSR V M+ Y+DSLV+P+GWL WQ + FA  TLYY EY+NFG
Sbjct: 430 LKPVVTAFKTYLGRPWMQYSRVVFMKCYLDSLVNPVGWLEWQRSNFALSTLYYAEYKNFG 489

Query: 491 PRASTRLRVRWPGFHAITTRNVASKFTVRRLIAGHTWLPATGVPFKLDL 535
           P +STR RV+WPGFH IT+ NVASKFTV  LIAG +WLPATGVP+   L
Sbjct: 490 PASSTRYRVKWPGFHVITSANVASKFTVSSLIAGRSWLPATGVPYTAGL 528

BLAST of CmaCh16G008360 vs. NCBI nr
Match: gi|658037270|ref|XP_008354203.1| (PREDICTED: probable pectinesterase/pectinesterase inhibitor 33 [Malus domestica])

HSP 1 Score: 632.1 bits (1629), Expect = 8.8e-178
Identity = 314/509 (61.69%), Postives = 386/509 (75.83%), Query Frame = 1

Query: 33  INKWCNTTPHPKVCIFFMSPL--KSPPQNRIEFRKMAIKTTLEGAAKAQNYVSRFGPTCK 92
           I  WC  TPHP+ C + +S    + PP++  EFRKM ++  LE A  A  Y  ++G  C+
Sbjct: 38  IRTWCRKTPHPESCNYCLSNSIHRFPPKHTSEFRKMLVQVALERALDAITYTFQYGQNCR 97

Query: 93  TTRQRAAWTDCFNLYNDTVFQLNRTIRCLHAGTRLRHCTDFDAQTWLSSALTNIDMCSSG 152
              +++AW DC  L+ +TV QLN T+  L  G + R C++FDAQTWLS+ALTNI  C  G
Sbjct: 98  NNNEKSAWADCLXLFENTVNQLNVTLEGL--GNK-RKCSNFDAQTWLSAALTNIHTCRVG 157

Query: 153 AADLNVTDFITPI--KCQNVSRMISNCLAINGRFLEGETKSRTTGAEEYQNG---FPRWV 212
           + +LNVTDFITPI     NVS +ISN LAI  + L         G EE  N    FP WV
Sbjct: 158 SLELNVTDFITPIYRSSNNVSELISNSLAIGSQLL---------GTEESYNESEKFPNWV 217

Query: 213 SSGDRKLLQSARVRAHLVVAKDGSGKFRTVQAAINAAARRRGRSRFVIYIKRGVYRENIE 272
           S  DR+LLQ++ +RA+LVVAKDGSG F TVQAA++AAARRR +S+F+IY+K+GVY ENI+
Sbjct: 218 SKHDRRLLQASNIRANLVVAKDGSGXFSTVQAALDAAARRRVKSKFIIYVKKGVYXENIQ 277

Query: 273 IGNDNANIMLVGDGIRFTVITSGRSVAAGFTTFNSATAGIQGPGFMARGIRFVNTAGPRM 332
           + N N+ IMLVG GIR+T+ITS RSV+ G+TT+NSATAGI G GF+ARGI F NTAGP  
Sbjct: 278 VSNTNSKIMLVGAGIRYTIITSSRSVSEGYTTYNSATAGINGXGFIARGITFSNTAGPLK 337

Query: 333 GQAVALRSSSDLSVFHRCSFEGYQDTLMVLSQRQFYKQCYVYGTIDFIFGNAAVVLQNCL 392
           GQAVALRS+SDLSVF+RC+F+GYQDTLMV SQRQFY++CY++GTIDFIFGNAA V QNC+
Sbjct: 338 GQAVALRSASDLSVFYRCAFQGYQDTLMVYSQRQFYRECYIFGTIDFIFGNAAAVFQNCM 397

Query: 393 IYVRRPLKGQVNVITAQGREDPFQNSGISIHNSQIRAAADLQPMIGSVKTYLGRPWKKYS 452
           IYVR+PL GQVNVITAQGR DPFQN+ ISIHNSQ+RAAADL+P+ GS KTYLGRPW +YS
Sbjct: 398 IYVRKPLWGQVNVITAQGRNDPFQNTAISIHNSQVRAAADLKPVAGSYKTYLGRPWMQYS 457

Query: 453 RTVIMRSYIDSLVSPMGWLAWQGTRFAQDTLYYGEYRNFGPRASTRLRVRWPGFHAITTR 512
           R V M+ Y+D+LV+P GWL WQ + FA  TLYYGEY+NFGP + TR RV+WPGFHAIT+ 
Sbjct: 458 RVVFMKCYLDNLVNPAGWLRWQRSNFALSTLYYGEYQNFGPGSCTRYRVKWPGFHAITSP 517

Query: 513 NVASKFTVRRLIAGHTWLPATGVPFKLDL 535
           NVAS FT   LIAG +WLP TGVPF + L
Sbjct: 518 NVASMFTAGSLIAGWSWLPTTGVPFTMGL 534