Carg24835 (gene) Silver-seed gourd

BLAST of Carg24835 vs. NCBI nr
Match: XP_022932129.1 (probable pectinesterase/pectinesterase inhibitor 33 [Cucurbita moschata])

HSP 1 Score: 1074.7 bits (2778), Expect = 1.6e-310
Identity = 531/534 (99.44%), Postives = 531/534 (99.44%), Query Frame = 0

Query: 1   MAPHIQIAFFSIALFALLSNSSSSPTKTSGNTINKWCNTTPHPKVCIFFMSPLKSPPQNR 60
           MAPHIQIAFFSIALFALLSNSSSSPTKTSGNTINKWCNTTPHPKVCIFFMSPLKSPPQNR
Sbjct: 1   MAPHIQIAFFSIALFALLSNSSSSPTKTSGNTINKWCNTTPHPKVCIFFMSPLKSPPQNR 60

Query: 61  LEFRKMAIKTTLEGAAKAQNYVSRFGPTCKTTRQRAAWTDCFNLYNDTVFQLNRTIQCLD 120
           LEFRKMAIKTTLEGAAKAQNYVS FGPTCKTTRQRAAWTDCFNLYNDTVFQLNRTIQCLD
Sbjct: 61  LEFRKMAIKTTLEGAAKAQNYVSLFGPTCKTTRQRAAWTDCFNLYNDTVFQLNRTIQCLD 120

Query: 121 GGNRLRHCTDFDAQTWLSSALTNIDMCSSGAADLNVTDFITPIKCQNVSKMISNCLAING 180
           GG RLRHCTDFDAQTWLSSALTNIDMCSSGAADLNVTDFITPIKCQNVSKMISNCLAING
Sbjct: 121 GGTRLRHCTDFDAQTWLSSALTNIDMCSSGAADLNVTDFITPIKCQNVSKMISNCLAING 180

Query: 181 RFLQEETKSRTMGTEEYQNGFPTWVSSGDRKLLQSARVRAHLVVAKDGSGKFRTVQAAIN 240
           RFLQEETKSRT GTEEYQNGFPTWVSSGDRKLLQSARVRAHLVVAKDGSGKFRTVQAAIN
Sbjct: 181 RFLQEETKSRTTGTEEYQNGFPTWVSSGDRKLLQSARVRAHLVVAKDGSGKFRTVQAAIN 240

Query: 241 AAARRRGRSRFVIYIKRGVYKENIEVGNDNANIMLVGDGIRFTVITSGRSVAAGFTTFNS 300
           AAARRRGRSRFVIYIKRGVYKENIEVGNDNANIMLVGDGIRFTVITSGRSVAAGFTTFNS
Sbjct: 241 AAARRRGRSRFVIYIKRGVYKENIEVGNDNANIMLVGDGIRFTVITSGRSVAAGFTTFNS 300

Query: 301 ATAGIQGPGFMARGIRFVNTAGPRMGQAVALRSSSDLSVFHRCSFEGYQDTLMVLSQRQF 360
           ATAGIQGPGFMARGIRFVNTAGPRMGQAVALRSSSDLSVFHRCSFEGYQDTLMVLSQRQF
Sbjct: 301 ATAGIQGPGFMARGIRFVNTAGPRMGQAVALRSSSDLSVFHRCSFEGYQDTLMVLSQRQF 360

Query: 361 YKQCYVYGTIDFIFGNAAVVLQNCLIYVRRPLKGQVNVITAQGREDPFQNSGISIHNSQI 420
           YKQCYVYGTIDFIFGNAAVVLQNCLIYVRRPLKGQVNVITAQGREDPFQNSGISIHNSQI
Sbjct: 361 YKQCYVYGTIDFIFGNAAVVLQNCLIYVRRPLKGQVNVITAQGREDPFQNSGISIHNSQI 420

Query: 421 RAAADLQPMIGSVKTYLGRPWKKYSRTVIMRSYIDSLVSPMGWLAWQGTRFAQDTLYYGE 480
           RAAADLQPMIGSVKTYLGRPWKKYSRTVIMRSYIDSLVSPMGWLAWQGTRFAQDTLYYGE
Sbjct: 421 RAAADLQPMIGSVKTYLGRPWKKYSRTVIMRSYIDSLVSPMGWLAWQGTRFAQDTLYYGE 480

Query: 481 YRNFGPRASTRLRVRWPGFHAITTRNVASKFTVRRLIAGHTWLPATGVPFKLDL 535
           YRNFGPRASTRLRVRWPGFHAITTRNVASKFTVRRLIAGHTWLPATGVPFKLDL
Sbjct: 481 YRNFGPRASTRLRVRWPGFHAITTRNVASKFTVRRLIAGHTWLPATGVPFKLDL 534

BLAST of Carg24835 vs. NCBI nr
Match: XP_023520442.1 (probable pectinesterase/pectinesterase inhibitor 59 isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1063.1 bits (2748), Expect = 3.0e-307
Identity = 524/534 (98.13%), Postives = 529/534 (99.06%), Query Frame = 0

Query: 1   MAPHIQIAFFSIALFALLSNSSSSPTKTSGNTINKWCNTTPHPKVCIFFMSPLKSPPQNR 60
           MAPHIQIAFFSIALFALLSNSSSS TKTS NTINKWCNTTPHPKVCIFFMSPLKSPPQNR
Sbjct: 51  MAPHIQIAFFSIALFALLSNSSSSLTKTSANTINKWCNTTPHPKVCIFFMSPLKSPPQNR 110

Query: 61  LEFRKMAIKTTLEGAAKAQNYVSRFGPTCKTTRQRAAWTDCFNLYNDTVFQLNRTIQCLD 120
           L+FRK+AIKTTLEGAAKAQNYVSRFGPTCKTTRQRAAWTDCFNLYNDTVFQLNRTIQCLD
Sbjct: 111 LQFRKLAIKTTLEGAAKAQNYVSRFGPTCKTTRQRAAWTDCFNLYNDTVFQLNRTIQCLD 170

Query: 121 GGNRLRHCTDFDAQTWLSSALTNIDMCSSGAADLNVTDFITPIKCQNVSKMISNCLAING 180
            G RLRHCTDFDAQTWLSSALTNIDMCSSGAADLNVTDFITPIKCQNVSKMISNCLAING
Sbjct: 171 AGTRLRHCTDFDAQTWLSSALTNIDMCSSGAADLNVTDFITPIKCQNVSKMISNCLAING 230

Query: 181 RFLQEETKSRTMGTEEYQNGFPTWVSSGDRKLLQSARVRAHLVVAKDGSGKFRTVQAAIN 240
           RFL+EETKSRT GTEEYQNGFPTW+S+GDRKLLQSARVRAHLVVAKDGSGKFRTVQAAIN
Sbjct: 231 RFLEEETKSRTTGTEEYQNGFPTWLSTGDRKLLQSARVRAHLVVAKDGSGKFRTVQAAIN 290

Query: 241 AAARRRGRSRFVIYIKRGVYKENIEVGNDNANIMLVGDGIRFTVITSGRSVAAGFTTFNS 300
           AAARRRGRSRFVIYIKRGVYKENIEVGNDNANIMLVGDGIRFTVITSGRSVAAGFTTFNS
Sbjct: 291 AAARRRGRSRFVIYIKRGVYKENIEVGNDNANIMLVGDGIRFTVITSGRSVAAGFTTFNS 350

Query: 301 ATAGIQGPGFMARGIRFVNTAGPRMGQAVALRSSSDLSVFHRCSFEGYQDTLMVLSQRQF 360
           ATAGIQGPGFMARGIRFVNTAGPRMGQAVALRSSSDLSVFHRCSFEGYQDTLMVLSQRQF
Sbjct: 351 ATAGIQGPGFMARGIRFVNTAGPRMGQAVALRSSSDLSVFHRCSFEGYQDTLMVLSQRQF 410

Query: 361 YKQCYVYGTIDFIFGNAAVVLQNCLIYVRRPLKGQVNVITAQGREDPFQNSGISIHNSQI 420
           YKQCYVYGTIDFIFGNAAVVLQNCLIYVRRPLKGQVNVITAQGREDPFQNSGISIHNSQI
Sbjct: 411 YKQCYVYGTIDFIFGNAAVVLQNCLIYVRRPLKGQVNVITAQGREDPFQNSGISIHNSQI 470

Query: 421 RAAADLQPMIGSVKTYLGRPWKKYSRTVIMRSYIDSLVSPMGWLAWQGTRFAQDTLYYGE 480
           RAAADLQPMIGSVKTYLGRPWKKYSRTVIMRSYIDSLVSPMGWLAWQGTRFAQDTLYYGE
Sbjct: 471 RAAADLQPMIGSVKTYLGRPWKKYSRTVIMRSYIDSLVSPMGWLAWQGTRFAQDTLYYGE 530

Query: 481 YRNFGPRASTRLRVRWPGFHAITTRNVASKFTVRRLIAGHTWLPATGVPFKLDL 535
           YRNFGPRASTRLRVRWPGFHAITTRNVASKFTVRRLIAGHTWLPATGVPFKLDL
Sbjct: 531 YRNFGPRASTRLRVRWPGFHAITTRNVASKFTVRRLIAGHTWLPATGVPFKLDL 584

BLAST of Carg24835 vs. NCBI nr
Match: XP_022985199.1 (probable pectinesterase/pectinesterase inhibitor 33 [Cucurbita maxima])

HSP 1 Score: 1057.0 bits (2732), Expect = 2.2e-305
Identity = 520/534 (97.38%), Postives = 526/534 (98.50%), Query Frame = 0

Query: 1   MAPHIQIAFFSIALFALLSNSSSSPTKTSGNTINKWCNTTPHPKVCIFFMSPLKSPPQNR 60
           MAPHIQIAFFSIALFALLSNSSSSPTKTS NTINKWCNTTPHPKVCIFFMSPLKSPPQNR
Sbjct: 1   MAPHIQIAFFSIALFALLSNSSSSPTKTSANTINKWCNTTPHPKVCIFFMSPLKSPPQNR 60

Query: 61  LEFRKMAIKTTLEGAAKAQNYVSRFGPTCKTTRQRAAWTDCFNLYNDTVFQLNRTIQCLD 120
           +EFRKMAIKTTLEGAAKAQNYVSRFGPTCKTTRQRAAWTDCFNLYNDTVFQLNRTI+CL 
Sbjct: 61  IEFRKMAIKTTLEGAAKAQNYVSRFGPTCKTTRQRAAWTDCFNLYNDTVFQLNRTIRCLH 120

Query: 121 GGNRLRHCTDFDAQTWLSSALTNIDMCSSGAADLNVTDFITPIKCQNVSKMISNCLAING 180
            G RLRHCTDFDAQTWLSSALTNIDMCSSGAADLNVTDFITPIKCQNVS+MISNCLAING
Sbjct: 121 AGTRLRHCTDFDAQTWLSSALTNIDMCSSGAADLNVTDFITPIKCQNVSRMISNCLAING 180

Query: 181 RFLQEETKSRTMGTEEYQNGFPTWVSSGDRKLLQSARVRAHLVVAKDGSGKFRTVQAAIN 240
           RFL+ ETKSRT G EEYQNGFP WVSSGDRKLLQSARVRAHLVVAKDGSGKFRTVQAAIN
Sbjct: 181 RFLEGETKSRTTGAEEYQNGFPRWVSSGDRKLLQSARVRAHLVVAKDGSGKFRTVQAAIN 240

Query: 241 AAARRRGRSRFVIYIKRGVYKENIEVGNDNANIMLVGDGIRFTVITSGRSVAAGFTTFNS 300
           AAARRRGRSRFVIYIKRGVY+ENIE+GNDNANIMLVGDGIRFTVITSGRSVAAGFTTFNS
Sbjct: 241 AAARRRGRSRFVIYIKRGVYRENIEIGNDNANIMLVGDGIRFTVITSGRSVAAGFTTFNS 300

Query: 301 ATAGIQGPGFMARGIRFVNTAGPRMGQAVALRSSSDLSVFHRCSFEGYQDTLMVLSQRQF 360
           ATAGIQGPGFMARGIRFVNTAGPRMGQAVALRSSSDLSVFHRCSFEGYQDTLMVLSQRQF
Sbjct: 301 ATAGIQGPGFMARGIRFVNTAGPRMGQAVALRSSSDLSVFHRCSFEGYQDTLMVLSQRQF 360

Query: 361 YKQCYVYGTIDFIFGNAAVVLQNCLIYVRRPLKGQVNVITAQGREDPFQNSGISIHNSQI 420
           YKQCYVYGTIDFIFGNAAVVLQNCLIYVRRPLKGQVNVITAQGREDPFQNSGISIHNSQI
Sbjct: 361 YKQCYVYGTIDFIFGNAAVVLQNCLIYVRRPLKGQVNVITAQGREDPFQNSGISIHNSQI 420

Query: 421 RAAADLQPMIGSVKTYLGRPWKKYSRTVIMRSYIDSLVSPMGWLAWQGTRFAQDTLYYGE 480
           RAAADLQPMIGSVKTYLGRPWKKYSRTVIMRSYIDSLVSPMGWLAWQGTRFAQDTLYYGE
Sbjct: 421 RAAADLQPMIGSVKTYLGRPWKKYSRTVIMRSYIDSLVSPMGWLAWQGTRFAQDTLYYGE 480

Query: 481 YRNFGPRASTRLRVRWPGFHAITTRNVASKFTVRRLIAGHTWLPATGVPFKLDL 535
           YRNFGPRASTRLRVRWPGFHAITTRNVASKFTVRRLIAGHTWLPATGVPFKLDL
Sbjct: 481 YRNFGPRASTRLRVRWPGFHAITTRNVASKFTVRRLIAGHTWLPATGVPFKLDL 534

BLAST of Carg24835 vs. NCBI nr
Match: XP_023520443.1 (probable pectinesterase/pectinesterase inhibitor 59 isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1046.6 bits (2705), Expect = 2.9e-302
Identity = 519/534 (97.19%), Postives = 524/534 (98.13%), Query Frame = 0

Query: 1   MAPHIQIAFFSIALFALLSNSSSSPTKTSGNTINKWCNTTPHPKVCIFFMSPLKSPPQNR 60
           MAPHIQIAFFSIALFALLSNSSSS TKTS NTINKWCNTTPHPKVCIFFMSPLKSPPQNR
Sbjct: 51  MAPHIQIAFFSIALFALLSNSSSSLTKTSANTINKWCNTTPHPKVCIFFMSPLKSPPQNR 110

Query: 61  LEFRKMAIKTTLEGAAKAQNYVSRFGPTCKTTRQRAAWTDCFNLYNDTVFQLNRTIQCLD 120
           L+FRK+AIKTTLEGAAKAQNYVSRFGPTCKTTRQRAAWTDCFNLYNDTVFQLNRTIQCLD
Sbjct: 111 LQFRKLAIKTTLEGAAKAQNYVSRFGPTCKTTRQRAAWTDCFNLYNDTVFQLNRTIQCLD 170

Query: 121 GGNRLRHCTDFDAQTWLSSALTNIDMCSSGAADLNVTDFITPIKCQNVSKMISNCLAING 180
            G RLRHCTDFDAQTWLSSALTNIDMCSSGAADLNVTDFITPIKCQNVSKMISNCLAING
Sbjct: 171 AGTRLRHCTDFDAQTWLSSALTNIDMCSSGAADLNVTDFITPIKCQNVSKMISNCLAING 230

Query: 181 RFLQEETKSRTMGTEEYQNGFPTWVSSGDRKLLQSARVRAHLVVAKDGSGKFRTVQAAIN 240
           RFL+EETKSRT GTEEYQNGFPTW+S+GDRKLLQSARVRAHLVVAKDGSGKFRTVQAAIN
Sbjct: 231 RFLEEETKSRTTGTEEYQNGFPTWLSTGDRKLLQSARVRAHLVVAKDGSGKFRTVQAAIN 290

Query: 241 AAARRRGRSRFVIYIKRGVYKENIEVGNDNANIMLVGDGIRFTVITSGRSVAAGFTTFNS 300
           AAARRRGRSRFVIYIKRGVYKENIEVGNDNANIMLVGDGIRFTVITSGRSVAAGFTTFNS
Sbjct: 291 AAARRRGRSRFVIYIKRGVYKENIEVGNDNANIMLVGDGIRFTVITSGRSVAAGFTTFNS 350

Query: 301 ATAGIQGPGFMARGIRFVNTAGPRMGQAVALRSSSDLSVFHRCSFEGYQDTLMVLSQRQF 360
           ATA     GFMARGIRFVNTAGPRMGQAVALRSSSDLSVFHRCSFEGYQDTLMVLSQRQF
Sbjct: 351 ATA-----GFMARGIRFVNTAGPRMGQAVALRSSSDLSVFHRCSFEGYQDTLMVLSQRQF 410

Query: 361 YKQCYVYGTIDFIFGNAAVVLQNCLIYVRRPLKGQVNVITAQGREDPFQNSGISIHNSQI 420
           YKQCYVYGTIDFIFGNAAVVLQNCLIYVRRPLKGQVNVITAQGREDPFQNSGISIHNSQI
Sbjct: 411 YKQCYVYGTIDFIFGNAAVVLQNCLIYVRRPLKGQVNVITAQGREDPFQNSGISIHNSQI 470

Query: 421 RAAADLQPMIGSVKTYLGRPWKKYSRTVIMRSYIDSLVSPMGWLAWQGTRFAQDTLYYGE 480
           RAAADLQPMIGSVKTYLGRPWKKYSRTVIMRSYIDSLVSPMGWLAWQGTRFAQDTLYYGE
Sbjct: 471 RAAADLQPMIGSVKTYLGRPWKKYSRTVIMRSYIDSLVSPMGWLAWQGTRFAQDTLYYGE 530

Query: 481 YRNFGPRASTRLRVRWPGFHAITTRNVASKFTVRRLIAGHTWLPATGVPFKLDL 535
           YRNFGPRASTRLRVRWPGFHAITTRNVASKFTVRRLIAGHTWLPATGVPFKLDL
Sbjct: 531 YRNFGPRASTRLRVRWPGFHAITTRNVASKFTVRRLIAGHTWLPATGVPFKLDL 579

BLAST of Carg24835 vs. NCBI nr
Match: XP_011650542.1 (PREDICTED: probable pectinesterase/pectinesterase inhibitor 59 [Cucumis sativus] >KGN56178.1 hypothetical protein Csa_3G088980 [Cucumis sativus])

HSP 1 Score: 855.5 bits (2209), Expect = 9.5e-245
Identity = 429/537 (79.89%), Postives = 468/537 (87.15%), Query Frame = 0

Query: 1   MAPHIQIAFFSIALFALLSNSSSSPTKTSGNTINKWCNTTPHPKVCIFFMSPLKSPPQNR 60
           MA H +I F SI  F LLSN+       S N I KWC TTPHP+VC+  MSP+K PPQNR
Sbjct: 1   MASHYKILFISITFFTLLSNN------VSANPIIKWCKTTPHPRVCVSLMSPIKPPPQNR 60

Query: 61  LEFRKMAIKTTLEGAAKAQNYVSRFGPTCKTTRQRAAWTDCFNLYNDTVFQLNRTIQCL- 120
            EFR+MAI+TTLE AA+A+ Y +RFGPTCKT+RQR AWTDCF LYND V QLNRT+ C+ 
Sbjct: 61  REFRQMAIQTTLEKAAEARAYTARFGPTCKTSRQRTAWTDCFKLYNDVVLQLNRTLHCVV 120

Query: 121 -DGGNRLRHCTDFDAQTWLSSALTNIDMCSSGAADLNVTDFITPIKCQNVSKMISNCLAI 180
            D     R CTDFDAQTWLSSALT+ID+C+SGAADLNVTDFITPIKC NVSKMISNCLAI
Sbjct: 121 TDEAIHRRSCTDFDAQTWLSSALTDIDLCNSGAADLNVTDFITPIKCLNVSKMISNCLAI 180

Query: 181 NGRFLQEETKSRTMGTEEYQNG-FPTWVSSGDRKLLQS--ARVRAHLVVAKDGSGKFRTV 240
           NG FL+EE     +  ++ +NG FP WVS GDRKLL+S   RVRA+LVVAKDGSG FR V
Sbjct: 181 NGGFLEEE----GVKYDDGRNGSFPMWVSEGDRKLLESRPGRVRANLVVAKDGSGTFRRV 240

Query: 241 QAAINAAARRRGRSRFVIYIKRGVYKENIEVGNDNANIMLVGDGIRFTVITSGRSVAAGF 300
           QAAI+AAARRRGR RF+IY+KRGVY+ENIEVGNDN NIMLVGDG+RFTVITSGRSVAAGF
Sbjct: 241 QAAIDAAARRRGRGRFIIYVKRGVYRENIEVGNDNGNIMLVGDGMRFTVITSGRSVAAGF 300

Query: 301 TTFNSATAGIQGPGFMARGIRFVNTAGPRMGQAVALRSSSDLSVFHRCSFEGYQDTLMVL 360
           TTF+SATAGIQGPGF+AR IRFVNTAGPRMGQAVALRSSSDLSVFHRCSFEGYQDTLMVL
Sbjct: 301 TTFSSATAGIQGPGFIARDIRFVNTAGPRMGQAVALRSSSDLSVFHRCSFEGYQDTLMVL 360

Query: 361 SQRQFYKQCYVYGTIDFIFGNAAVVLQNCLIYVRRPLKGQVNVITAQGREDPFQNSGISI 420
           SQRQFYKQCYVYGTIDFIFGNAAVVLQNC+IYVRRPLKGQVNVITAQGREDPFQNSGISI
Sbjct: 361 SQRQFYKQCYVYGTIDFIFGNAAVVLQNCMIYVRRPLKGQVNVITAQGREDPFQNSGISI 420

Query: 421 HNSQIRAAADLQPMIGSVKTYLGRPWKKYSRTVIMRSYIDSLVSPMGWLAWQGTRFAQDT 480
           HNSQIRAAADL+PM+GSVKTYLGRPWKKYSRTVIMRSYID LVSP GWLAWQ ++FAQ T
Sbjct: 421 HNSQIRAAADLRPMVGSVKTYLGRPWKKYSRTVIMRSYIDWLVSPAGWLAWQSSKFAQAT 480

Query: 481 LYYGEYRNFGPRASTRLRVRWPGFHAITTRNVASKFTVRRLIAGHTWLPATGVPFKL 533
           LYYGEYRN GPRASTR RV+WPGFH I + NVASKF+V+RLIAG TWLPATGVPFKL
Sbjct: 481 LYYGEYRNIGPRASTRFRVKWPGFHVIKSPNVASKFSVQRLIAGQTWLPATGVPFKL 527

BLAST of Carg24835 vs. TAIR10
Match: AT3G47400.1 (Plant invertase/pectin methylesterase inhibitor superfamily)

HSP 1 Score: 598.2 bits (1541), Expect = 5.0e-171
Identity = 297/511 (58.12%), Postives = 384/511 (75.15%), Query Frame = 0

Query: 36  WCNTTPHPKVCIFFMSPLKS------PPQNRLEFRKMAIKTTLEGAAKAQNYVSRFGPTC 95
           WCN TPH + C ++            PP+ R EF +M +K  L+ A    +   +FGP+C
Sbjct: 87  WCNKTPHAETCNYYFRKSSQNNINLRPPRFRSEFLRMLVKVALDQAVITHSQTVKFGPSC 146

Query: 96  KTTRQRAAWTDCFNLYNDTVFQLNRTIQCLD-GGNRLRHCTDFDAQTWLSSALTNIDMCS 155
              +++AAW+DC NL+ +TV QLNRT++ L+   +    CTDFDAQTWLS+A TNI+ C 
Sbjct: 147 TNNQRKAAWSDCVNLFQNTVAQLNRTLKGLNPAASSDVKCTDFDAQTWLSTAQTNIETCR 206

Query: 156 SGAADLNVTDFITP-IKCQNVSKMISNCLAINGRFLQEETKSRTMGTEEYQNGFPTWVSS 215
           SG+ DLNV+DF+ P I  +N+S +I NCLA+NG  +++   + T   +EY   FP+WVS 
Sbjct: 207 SGSEDLNVSDFVMPVISNKNLSDLIGNCLAVNGVLMKQHDHTTTANHKEY---FPSWVSR 266

Query: 216 GDRKLLQS---ARVRAHLVVAKDGSGKFRTVQAAINAAARRRGRSRFVIYIKRGVYKENI 275
            +R+LL S   A+   HLVVA+D SG FR++QAAIN AARRR +SRFVIY+K+GVY+ENI
Sbjct: 267 HERRLLVSASLAKSSPHLVVAQDRSGHFRSIQAAINFAARRRFKSRFVIYVKKGVYRENI 326

Query: 276 EVGNDNANIMLVGDGIRFTVITSGRSVAAGFTTFNSATAGIQGPGFMARGIRFVNTAGPR 335
           +VGNDN NIMLVGDG R T+ITSGRSV  G+TT+NSAT G  G  F+A+ + F+NTAGP 
Sbjct: 327 DVGNDNHNIMLVGDGERKTIITSGRSVQHGYTTYNSATGGFGGQRFVAKDMTFINTAGPL 386

Query: 336 MGQAVALRSSSDLSVFHRCSFEGYQDTLMVLSQRQFYKQCYVYGTIDFIFGNAAVVLQNC 395
            GQAVA+RSSSDLSVF+R    G+QDTL + SQRQF+++CY+ GTIDFIFGNAAVV QNC
Sbjct: 387 RGQAVAVRSSSDLSVFYRVGIHGFQDTLYIHSQRQFFRECYISGTIDFIFGNAAVVFQNC 446

Query: 396 LIYVRRPLKGQVNVITAQGREDPFQNSGISIHNSQIRAAADLQPMIGSVKTYLGRPWKKY 455
           +I VRRPL GQ N+ITAQGR DPFQN+GI+IH+S+I AA+DL+P+I + KTYLGRPW+ Y
Sbjct: 447 MILVRRPLHGQANIITAQGRGDPFQNTGITIHSSRIIAASDLKPVIRAYKTYLGRPWQAY 506

Query: 456 SRTVIMRSYIDSLVSPMGWLAW-QGTRFAQDTLYYGEYRNFGPRASTRLRVRWPGFHAIT 515
           SR  IM++YID+ +SP+GW  W +G+ FA +T++YGEY+NFGP +STR RVRW GFHAIT
Sbjct: 507 SRVTIMKTYIDNSISPLGWSPWLRGSNFALNTVFYGEYKNFGPGSSTRWRVRWKGFHAIT 566

Query: 516 TRNVASKFTVRRLIAGHTWLPATGVPFKLDL 535
           +  VAS+FTV  LIAG +WLPATGVPFK  L
Sbjct: 567 STAVASRFTVGSLIAGGSWLPATGVPFKSGL 594

BLAST of Carg24835 vs. TAIR10
Match: AT5G51490.1 (Plant invertase/pectin methylesterase inhibitor superfamily)

HSP 1 Score: 578.6 bits (1490), Expect = 4.1e-165
Identity = 296/536 (55.22%), Postives = 378/536 (70.52%), Query Frame = 0

Query: 1   MAPHIQIAFFSIALFALLSNSSSSPTKTSGNTINKWCNTTPHPKVCIFFMSPLK--SPPQ 60
           M   + I F  + L  LL     +      +T   WC+ TP+P  C  +         P 
Sbjct: 4   MMQKLSILFLHLILLVLLCVHPLTTVADRNST--DWCDKTPYPDPCKCYFKNHNGFQQPT 63

Query: 61  NRLEFRKMAIKTTLEGAAKAQNYVSRFGPTCKTTRQRAAWTDCFNLYNDTVFQLNRTIQC 120
              EFR M ++  ++ A  A+  ++  G  C  ++++A   DC +LY DT+ QLNRT+  
Sbjct: 64  QLSEFRVMLVEAAMDRAISARAELTNSGKNCTDSKKQAVLADCIDLYGDTIMQLNRTLHG 123

Query: 121 LD-GGNRLRHCTDFDAQTWLSSALTNIDMCSSGAADLNVTDFITPIKCQ-NVSKMISNCL 180
           +       + CTDFDAQTWLS+ALTN + C  G++DLNVTDFITPI     +S +ISNCL
Sbjct: 124 VSPKAGAAKSCTDFDAQTWLSTALTNTETCRRGSSDLNVTDFITPIVSNTKISHLISNCL 183

Query: 181 AINGRFLQEETKSRTMGTEEYQNGFPTWVSSGDRKLLQSARVRAHLVVAKDGSGKFRTVQ 240
           A+NG  L    K  T      Q GFPTW+S  D++LL++  VRA+LVVAKDGSG F TVQ
Sbjct: 184 AVNGALLTAGNKGNTTAN---QKGFPTWLSRKDKRLLRA--VRANLVVAKDGSGHFNTVQ 243

Query: 241 AAINAAARRRGRS-RFVIYIKRGVYKENIEVGNDNANIMLVGDGIRFTVITSGRSVAAGF 300
           AAI+ A RR+  S RFVIY+KRG+Y+ENI V  +N +IMLVGDG+R T+IT GRSV  G+
Sbjct: 244 AAIDVAGRRKVTSGRFVIYVKRGIYQENINVRLNNDDIMLVGDGMRSTIITGGRSVQGGY 303

Query: 301 TTFNSATAGIQGPGFMARGIRFVNTAGPRMGQAVALRSSSDLSVFHRCSFEGYQDTLMVL 360
           TT+NSATAGI+G  F+A+GI F NTAGP  GQAVALRSSSDLS+F++CS EGYQDTLMV 
Sbjct: 304 TTYNSATAGIEGLHFIAKGITFRNTAGPAKGQAVALRSSSDLSIFYKCSIEGYQDTLMVH 363

Query: 361 SQRQFYKQCYVYGTIDFIFGNAAVVLQNCLIYVRRPLKGQVNVITAQGREDPFQNSGISI 420
           SQRQFY++CY+YGT+DFIFGNAA V QNCLI  RRPLKGQ NVITAQGR DPFQN+GISI
Sbjct: 364 SQRQFYRECYIYGTVDFIFGNAAAVFQNCLILPRRPLKGQANVITAQGRADPFQNTGISI 423

Query: 421 HNSQIRAAADLQPMIGSVKTYLGRPWKKYSRTVIMRSYIDSLVSPMGWLAW-QGTRFAQD 480
           HNS+I  A DL+P++G+VKTY+GRPW K+SRTV++++Y+D++VSP+GW  W +G+ F  D
Sbjct: 424 HNSRILPAPDLKPVVGTVKTYMGRPWMKFSRTVVLQTYLDNVVSPVGWSPWIEGSVFGLD 483

Query: 481 TLYYGEYRNFGPRASTRLRVRWPGFHAITTRNVASKFTVRRLIAGHTWLPATGVPF 531
           TL+Y EY+N GP +STR RV W GFH +   + AS FTV + IAG  WLP TG+PF
Sbjct: 484 TLFYAEYKNTGPASSTRWRVSWKGFHVLGRASDASAFTVGKFIAGTAWLPRTGIPF 532

BLAST of Carg24835 vs. TAIR10
Match: AT5G51500.1 (Plant invertase/pectin methylesterase inhibitor superfamily)

HSP 1 Score: 577.8 bits (1488), Expect = 7.0e-165
Identity = 301/537 (56.05%), Postives = 383/537 (71.32%), Query Frame = 0

Query: 7   IAFFSI-ALFALLSNSSSSPTKTSGNT--INKWCNTTPHPKVCIFFM---SPLKSPPQNR 66
           I+F S+  L  LL       T   GN+  I+ WC+ TP+P  C  +    S  + P Q  
Sbjct: 9   ISFLSLHLLLILLLCLRPLTTVADGNSTNIDGWCDKTPYPYPCKRYFIKHSGFRLPTQIS 68

Query: 67  LEFRKMAIKTTLEGAAKAQNYVSRFGPTCKTTRQRAAWTDCFNLYNDTVFQLNRTIQCLD 126
            EFR + ++  ++ A  A + ++     C   +++A   DC NLY DTV QLNRT+Q + 
Sbjct: 69  -EFRVLLVEAAMDRAVSAWDKLTNSSKNCTDFKKQAVLADCINLYGDTVMQLNRTLQGVS 128

Query: 127 GGNRLRHCTDFDAQTWLSSALTNIDMCSSGAADLNVTDFITPIKCQ-NVSKMISNCLAIN 186
                R CTDFDAQTWLS+ALTN + C  G++DLNV+DF TPI     +S +ISNCLA+N
Sbjct: 129 SKTG-RRCTDFDAQTWLSTALTNTETCRRGSSDLNVSDFTTPIVSNTKISHLISNCLAVN 188

Query: 187 GRFLQEETKSRTMGTEEYQNGFPTWVSSGDRKLLQSARVRAHLVVAKDGSGKFRTVQAAI 246
           G  L       T G  +   GFPTWVS  +R+LLQ   VRA+LVVAKDGSG F+TVQAAI
Sbjct: 189 GALLTAGKNDSTTGDSK---GFPTWVSRKERRLLQLQSVRANLVVAKDGSGHFKTVQAAI 248

Query: 247 NAAARRRGRS-RFVIYIKRGVYKENIEVGNDNANIMLVGDGIRFTVITSGRSVAAGFTTF 306
           + A RR+  S RFVIY+KRG+Y+EN+ V  +N NIMLVGDG+R+T+IT GRSV  G+TT+
Sbjct: 249 DVAGRRKVTSGRFVIYVKRGIYQENLNVRLNNDNIMLVGDGMRYTIITGGRSVKGGYTTY 308

Query: 307 NSATAGIQGPGFMARGIRFVNTAGPRMGQAVALRSSSDLSVFHRCSFEGYQDTLMVLSQR 366
           +SATAGI+G  F+A+GI F NTAGP  GQAVALRSSSDLS+F+RCS EGYQDTLMV SQR
Sbjct: 309 SSATAGIEGLHFIAKGIAFQNTAGPAKGQAVALRSSSDLSIFYRCSIEGYQDTLMVHSQR 368

Query: 367 QFYKQCYVYGTIDFIFGNAAVVLQNCLIYVRRPLKGQVNVITAQGREDPFQNSGISIHNS 426
           QFY++CY+YGT+DFIFGNAAVV QNC+I  R PLKGQ NVITAQGR D FQN+GISIHNS
Sbjct: 369 QFYRECYIYGTVDFIFGNAAVVFQNCIILPRLPLKGQANVITAQGRTDLFQNTGISIHNS 428

Query: 427 QIRAAADLQPMIGSVKTYLGRPWKKYSRTVIMRSYIDSLVSPMGWLAW-QGTRFAQDTLY 486
            I  A DL+P++ SVKTY+GRPW  YSRTV++++YIDS+VSP+GW  W +G+ +  DTL+
Sbjct: 429 IIIPAPDLKPVVRSVKTYMGRPWMMYSRTVVLKTYIDSVVSPVGWSPWTKGSTYGLDTLF 488

Query: 487 YGEYRNFGPRASTRLRVRWPGFHAITTRNVASKFTVRRLIAGHTWLPATGVPFKLDL 535
           Y EY+N GP +STR RVRW GFH ++  + AS F+V + IAG  WLP +G+PF  +L
Sbjct: 489 YAEYKNIGPASSTRWRVRWKGFHVLSKASDASAFSVGKFIAGTAWLPGSGIPFTSEL 540

BLAST of Carg24835 vs. TAIR10
Match: AT2G45220.1 (Plant invertase/pectin methylesterase inhibitor superfamily)

HSP 1 Score: 507.7 bits (1306), Expect = 8.8e-144
Identity = 263/500 (52.60%), Postives = 344/500 (68.80%), Query Frame = 0

Query: 33  INKWCNTTPHPKVCIFFM--SPLKSPPQNRLEFRKMAIKTTLEGAAKAQNYVSRFGPTCK 92
           +  WC+ TP+PK C +F+  +    P ++  EF K+++K  L+ A  A+ +    GP C+
Sbjct: 30  VKAWCSQTPNPKPCEYFLTHNSNNEPIKSESEFLKISMKLVLDRAILAKTHAFTLGPKCR 89

Query: 93  TTRQRAAWTDCFNLYNDTVFQLNRTIQCLDGGNRLRHCTDFDAQTWLSSALTNIDMCSSG 152
            TR++AAW DC  LY+ TV ++N T   +D   +   C+  DAQTWLS+ALTN+D C +G
Sbjct: 90  DTREKAAWEDCIKLYDLTVSKINET---MDPNVK---CSKLDAQTWLSTALTNLDTCRAG 149

Query: 153 AADLNVTDFITPIKCQNVSKMISNCLAINGRFLQEETKSRTMGTEEYQNGFPTWVSSGDR 212
             +L VTD + P+   NVS ++ N LAIN        K     T   ++GFP+WV  GDR
Sbjct: 150 FLELGVTDIVLPLMSNNVSNLLCNTLAIN--------KVPFNYTPPEKDGFPSWVKPGDR 209

Query: 213 KLLQSARVRAHLVVAKDGSGKFRTVQAAINAAARRRGRSRFVIYIKRGVYKENIEVGNDN 272
           KLLQS+  + + VVAKDGSG F+T++ AI+AA+   G  RFVIY+K+GVY EN+E+    
Sbjct: 210 KLLQSSTPKDNAVVAKDGSGNFKTIKEAIDAAS---GSGRFVIYVKQGVYSENLEI--RK 269

Query: 273 ANIMLVGDGIRFTVITSGRSVAAGFTTFNSATAGIQGPGFMARGIRFVNTAGPRMGQAVA 332
            N+ML GDGI  T+IT  +SV  G TTFNSAT    G GF+ARGI F NTAG    QAVA
Sbjct: 270 KNVMLRGDGIGKTIITGSKSVGGGTTTFNSATVAAVGDGFIARGITFRNTAGASNEQAVA 329

Query: 333 LRSSSDLSVFHRCSFEGYQDTLMVLSQRQFYKQCYVYGTIDFIFGNAAVVLQNCLIYVRR 392
           LRS SDLSVF++CSFE YQDTL V S RQFY+ C VYGT+DFIFGNAA VLQNC I+ RR
Sbjct: 330 LRSGSDLSVFYQCSFEAYQDTLYVHSNRQFYRDCDVYGTVDFIFGNAAAVLQNCNIFARR 389

Query: 393 PLKGQVNVITAQGREDPFQNSGISIHNSQIRAAADLQPMIGSVKTYLGRPWKKYSRTVIM 452
           P + + N ITAQGR DP QN+GI IHNS++ AA+DL+P++GS KTYLGRPW++YSRTV M
Sbjct: 390 P-RSKTNTITAQGRSDPNQNTGIIIHNSRVTAASDLRPVLGSTKTYLGRPWRQYSRTVFM 449

Query: 453 RSYIDSLVSPMGWLAWQGTRFAQDTLYYGEYRNFGPRASTRLRVRWPGFHAITTRNVASK 512
           ++ +DSL+ P GWL W G  FA  TL+Y E++N GP AST  RV WPGF  + + + ASK
Sbjct: 450 KTSLDSLIDPRGWLEWDG-NFALKTLFYAEFQNTGPGASTSGRVTWPGFRVLGSASEASK 507

Query: 513 FTVRRLIAGHTWLPATGVPF 531
           FTV   +AG +W+P++ VPF
Sbjct: 510 FTVGTFLAGGSWIPSS-VPF 507

BLAST of Carg24835 vs. TAIR10
Match: AT4G00190.1 (pectin methylesterase 38)

HSP 1 Score: 404.1 bits (1037), Expect = 1.4e-112
Identity = 221/502 (44.02%), Postives = 310/502 (61.75%), Query Frame = 0

Query: 34  NKWCNTTPHPKVCIFFMSPLKSPPQNRLEFRKMAIKTTLEGAAKAQNYVSRFGPTCKTTR 93
           N+ C+ TPHP  C   +   K P ++  +F +++++ TL+GA KA++      P   +  
Sbjct: 5   NEMCDETPHPGECKTLLIKHK-PIRSTTQFLQVSVERTLDGAVKAKSDTYFLEPQFGS-- 64

Query: 94  QRAAWTDCFNLYNDTVFQLNRTIQCLDGGNRLRHCTDFDAQTWLSSALTNIDMCSSGAAD 153
            + AW +C +LY  T+ +LN ++ C         C+  D Q WLS+ALTN+D C    ++
Sbjct: 65  -KQAWEECMDLYEQTIHRLNESVLC-----PKNVCSRSDVQAWLSTALTNLDTCQEEMSE 124

Query: 154 LNVTDFITPIKCQNVSKMISNCLAINGRFLQEETKSRTMGTEEYQNGFPTWVSSGDRKLL 213
           L V+        ++++  + N LAIN R                QNG    +S    K L
Sbjct: 125 LGVSSH----SLESITIDVINTLAINKRM--------------EQNGKEFGISKITMKTL 184

Query: 214 QSARVRAHLVVAKDGSGKFRTVQAAINAAARR-RGRSRFVIYIKRGVYKENIEVGNDNAN 273
            S   +  +VVA+DGSG ++T+Q A+N A  R +G  R+VI++K+GVY+E + VG  + N
Sbjct: 185 -SIGEKVDVVVAQDGSGDYKTIQEAVNGAGERLKGSPRYVIHVKQGVYEEYVNVGIKSNN 244

Query: 274 IMLVGDGIRFTVITSGRSVAAGFTTFNSATAGIQGPGFMARGIRFVNTAGPRMGQAVALR 333
           IM+ GDGI  T+IT  +S   GF+T+ SAT   +G GF+ R I   NTAGP   QAVALR
Sbjct: 245 IMITGDGIGKTIITGDKSKGRGFSTYKSATFVAEGDGFVGRDITIRNTAGPENHQAVALR 304

Query: 334 SSSDLSVFHRCSFEGYQDTLMVLSQRQFYKQCYVYGTIDFIFGNAAVVLQNCLIYVRRPL 393
           S+SD+SVF+RCS EGYQDTL V S RQF+++C +YGT+DFIFGNAA VLQNC I+ R P 
Sbjct: 305 SNSDMSVFYRCSIEGYQDTLYVHSGRQFFRECDIYGTVDFIFGNAAAVLQNCRIFARNPP 364

Query: 394 KGQVNVITAQGREDPFQNSGISIHNSQIRAAADLQPMIGSVKTYLGRPWKKYSRTVIMRS 453
            G VN ITAQ R +P Q +GI IHNS ++ A  +Q  +G VKTYLGRPW+ Y+RTV++ +
Sbjct: 365 NG-VNTITAQSRFNPNQTTGIVIHNSVVKGAPGVQ--LGGVKTYLGRPWRSYARTVVIGT 424

Query: 454 YIDSLVSPMGWLAWQGTRFAQDTLYYGEYRNFGPRASTRLRVRWPGFHAITTRNVASKFT 513
           Y+D+L+ P GW+ W     A  TLYYGEY+N GP + T  RV W GFH I+    A +FT
Sbjct: 425 YLDTLIEPNGWIDWDNVT-ALSTLYYGEYQNSGPGSGTENRVDWAGFHVISDIQEAREFT 474

Query: 514 VRRLIAGHTWLPATGVPFKLDL 535
           + + I   +WLP T VPF ++L
Sbjct: 485 LPKFIDSASWLPPTKVPFTINL 474

BLAST of Carg24835 vs. Swiss-Prot
Match: sp|Q9STY3|PME33_ARATH (Probable pectinesterase/pectinesterase inhibitor 33 OS=Arabidopsis thaliana OX=3702 GN=PME33 PE=2 SV=1)

HSP 1 Score: 598.2 bits (1541), Expect = 9.0e-170
Identity = 297/511 (58.12%), Postives = 384/511 (75.15%), Query Frame = 0

Query: 36  WCNTTPHPKVCIFFMSPLKS------PPQNRLEFRKMAIKTTLEGAAKAQNYVSRFGPTC 95
           WCN TPH + C ++            PP+ R EF +M +K  L+ A    +   +FGP+C
Sbjct: 87  WCNKTPHAETCNYYFRKSSQNNINLRPPRFRSEFLRMLVKVALDQAVITHSQTVKFGPSC 146

Query: 96  KTTRQRAAWTDCFNLYNDTVFQLNRTIQCLD-GGNRLRHCTDFDAQTWLSSALTNIDMCS 155
              +++AAW+DC NL+ +TV QLNRT++ L+   +    CTDFDAQTWLS+A TNI+ C 
Sbjct: 147 TNNQRKAAWSDCVNLFQNTVAQLNRTLKGLNPAASSDVKCTDFDAQTWLSTAQTNIETCR 206

Query: 156 SGAADLNVTDFITP-IKCQNVSKMISNCLAINGRFLQEETKSRTMGTEEYQNGFPTWVSS 215
           SG+ DLNV+DF+ P I  +N+S +I NCLA+NG  +++   + T   +EY   FP+WVS 
Sbjct: 207 SGSEDLNVSDFVMPVISNKNLSDLIGNCLAVNGVLMKQHDHTTTANHKEY---FPSWVSR 266

Query: 216 GDRKLLQS---ARVRAHLVVAKDGSGKFRTVQAAINAAARRRGRSRFVIYIKRGVYKENI 275
            +R+LL S   A+   HLVVA+D SG FR++QAAIN AARRR +SRFVIY+K+GVY+ENI
Sbjct: 267 HERRLLVSASLAKSSPHLVVAQDRSGHFRSIQAAINFAARRRFKSRFVIYVKKGVYRENI 326

Query: 276 EVGNDNANIMLVGDGIRFTVITSGRSVAAGFTTFNSATAGIQGPGFMARGIRFVNTAGPR 335
           +VGNDN NIMLVGDG R T+ITSGRSV  G+TT+NSAT G  G  F+A+ + F+NTAGP 
Sbjct: 327 DVGNDNHNIMLVGDGERKTIITSGRSVQHGYTTYNSATGGFGGQRFVAKDMTFINTAGPL 386

Query: 336 MGQAVALRSSSDLSVFHRCSFEGYQDTLMVLSQRQFYKQCYVYGTIDFIFGNAAVVLQNC 395
            GQAVA+RSSSDLSVF+R    G+QDTL + SQRQF+++CY+ GTIDFIFGNAAVV QNC
Sbjct: 387 RGQAVAVRSSSDLSVFYRVGIHGFQDTLYIHSQRQFFRECYISGTIDFIFGNAAVVFQNC 446

Query: 396 LIYVRRPLKGQVNVITAQGREDPFQNSGISIHNSQIRAAADLQPMIGSVKTYLGRPWKKY 455
           +I VRRPL GQ N+ITAQGR DPFQN+GI+IH+S+I AA+DL+P+I + KTYLGRPW+ Y
Sbjct: 447 MILVRRPLHGQANIITAQGRGDPFQNTGITIHSSRIIAASDLKPVIRAYKTYLGRPWQAY 506

Query: 456 SRTVIMRSYIDSLVSPMGWLAW-QGTRFAQDTLYYGEYRNFGPRASTRLRVRWPGFHAIT 515
           SR  IM++YID+ +SP+GW  W +G+ FA +T++YGEY+NFGP +STR RVRW GFHAIT
Sbjct: 507 SRVTIMKTYIDNSISPLGWSPWLRGSNFALNTVFYGEYKNFGPGSSTRWRVRWKGFHAIT 566

Query: 516 TRNVASKFTVRRLIAGHTWLPATGVPFKLDL 535
           +  VAS+FTV  LIAG +WLPATGVPFK  L
Sbjct: 567 STAVASRFTVGSLIAGGSWLPATGVPFKSGL 594

BLAST of Carg24835 vs. Swiss-Prot
Match: sp|Q9FHN5|PME59_ARATH (Probable pectinesterase/pectinesterase inhibitor 59 OS=Arabidopsis thaliana OX=3702 GN=PME59 PE=2 SV=1)

HSP 1 Score: 578.6 bits (1490), Expect = 7.4e-164
Identity = 296/536 (55.22%), Postives = 378/536 (70.52%), Query Frame = 0

Query: 1   MAPHIQIAFFSIALFALLSNSSSSPTKTSGNTINKWCNTTPHPKVCIFFMSPLK--SPPQ 60
           M   + I F  + L  LL     +      +T   WC+ TP+P  C  +         P 
Sbjct: 4   MMQKLSILFLHLILLVLLCVHPLTTVADRNST--DWCDKTPYPDPCKCYFKNHNGFQQPT 63

Query: 61  NRLEFRKMAIKTTLEGAAKAQNYVSRFGPTCKTTRQRAAWTDCFNLYNDTVFQLNRTIQC 120
              EFR M ++  ++ A  A+  ++  G  C  ++++A   DC +LY DT+ QLNRT+  
Sbjct: 64  QLSEFRVMLVEAAMDRAISARAELTNSGKNCTDSKKQAVLADCIDLYGDTIMQLNRTLHG 123

Query: 121 LD-GGNRLRHCTDFDAQTWLSSALTNIDMCSSGAADLNVTDFITPIKCQ-NVSKMISNCL 180
           +       + CTDFDAQTWLS+ALTN + C  G++DLNVTDFITPI     +S +ISNCL
Sbjct: 124 VSPKAGAAKSCTDFDAQTWLSTALTNTETCRRGSSDLNVTDFITPIVSNTKISHLISNCL 183

Query: 181 AINGRFLQEETKSRTMGTEEYQNGFPTWVSSGDRKLLQSARVRAHLVVAKDGSGKFRTVQ 240
           A+NG  L    K  T      Q GFPTW+S  D++LL++  VRA+LVVAKDGSG F TVQ
Sbjct: 184 AVNGALLTAGNKGNTTAN---QKGFPTWLSRKDKRLLRA--VRANLVVAKDGSGHFNTVQ 243

Query: 241 AAINAAARRRGRS-RFVIYIKRGVYKENIEVGNDNANIMLVGDGIRFTVITSGRSVAAGF 300
           AAI+ A RR+  S RFVIY+KRG+Y+ENI V  +N +IMLVGDG+R T+IT GRSV  G+
Sbjct: 244 AAIDVAGRRKVTSGRFVIYVKRGIYQENINVRLNNDDIMLVGDGMRSTIITGGRSVQGGY 303

Query: 301 TTFNSATAGIQGPGFMARGIRFVNTAGPRMGQAVALRSSSDLSVFHRCSFEGYQDTLMVL 360
           TT+NSATAGI+G  F+A+GI F NTAGP  GQAVALRSSSDLS+F++CS EGYQDTLMV 
Sbjct: 304 TTYNSATAGIEGLHFIAKGITFRNTAGPAKGQAVALRSSSDLSIFYKCSIEGYQDTLMVH 363

Query: 361 SQRQFYKQCYVYGTIDFIFGNAAVVLQNCLIYVRRPLKGQVNVITAQGREDPFQNSGISI 420
           SQRQFY++CY+YGT+DFIFGNAA V QNCLI  RRPLKGQ NVITAQGR DPFQN+GISI
Sbjct: 364 SQRQFYRECYIYGTVDFIFGNAAAVFQNCLILPRRPLKGQANVITAQGRADPFQNTGISI 423

Query: 421 HNSQIRAAADLQPMIGSVKTYLGRPWKKYSRTVIMRSYIDSLVSPMGWLAW-QGTRFAQD 480
           HNS+I  A DL+P++G+VKTY+GRPW K+SRTV++++Y+D++VSP+GW  W +G+ F  D
Sbjct: 424 HNSRILPAPDLKPVVGTVKTYMGRPWMKFSRTVVLQTYLDNVVSPVGWSPWIEGSVFGLD 483

Query: 481 TLYYGEYRNFGPRASTRLRVRWPGFHAITTRNVASKFTVRRLIAGHTWLPATGVPF 531
           TL+Y EY+N GP +STR RV W GFH +   + AS FTV + IAG  WLP TG+PF
Sbjct: 484 TLFYAEYKNTGPASSTRWRVSWKGFHVLGRASDASAFTVGKFIAGTAWLPRTGIPF 532

BLAST of Carg24835 vs. Swiss-Prot
Match: sp|Q9FHN4|PME60_ARATH (Probable pectinesterase/pectinesterase inhibitor 60 OS=Arabidopsis thaliana OX=3702 GN=PME60 PE=2 SV=1)

HSP 1 Score: 577.8 bits (1488), Expect = 1.3e-163
Identity = 301/537 (56.05%), Postives = 383/537 (71.32%), Query Frame = 0

Query: 7   IAFFSI-ALFALLSNSSSSPTKTSGNT--INKWCNTTPHPKVCIFFM---SPLKSPPQNR 66
           I+F S+  L  LL       T   GN+  I+ WC+ TP+P  C  +    S  + P Q  
Sbjct: 9   ISFLSLHLLLILLLCLRPLTTVADGNSTNIDGWCDKTPYPYPCKRYFIKHSGFRLPTQIS 68

Query: 67  LEFRKMAIKTTLEGAAKAQNYVSRFGPTCKTTRQRAAWTDCFNLYNDTVFQLNRTIQCLD 126
            EFR + ++  ++ A  A + ++     C   +++A   DC NLY DTV QLNRT+Q + 
Sbjct: 69  -EFRVLLVEAAMDRAVSAWDKLTNSSKNCTDFKKQAVLADCINLYGDTVMQLNRTLQGVS 128

Query: 127 GGNRLRHCTDFDAQTWLSSALTNIDMCSSGAADLNVTDFITPIKCQ-NVSKMISNCLAIN 186
                R CTDFDAQTWLS+ALTN + C  G++DLNV+DF TPI     +S +ISNCLA+N
Sbjct: 129 SKTG-RRCTDFDAQTWLSTALTNTETCRRGSSDLNVSDFTTPIVSNTKISHLISNCLAVN 188

Query: 187 GRFLQEETKSRTMGTEEYQNGFPTWVSSGDRKLLQSARVRAHLVVAKDGSGKFRTVQAAI 246
           G  L       T G  +   GFPTWVS  +R+LLQ   VRA+LVVAKDGSG F+TVQAAI
Sbjct: 189 GALLTAGKNDSTTGDSK---GFPTWVSRKERRLLQLQSVRANLVVAKDGSGHFKTVQAAI 248

Query: 247 NAAARRRGRS-RFVIYIKRGVYKENIEVGNDNANIMLVGDGIRFTVITSGRSVAAGFTTF 306
           + A RR+  S RFVIY+KRG+Y+EN+ V  +N NIMLVGDG+R+T+IT GRSV  G+TT+
Sbjct: 249 DVAGRRKVTSGRFVIYVKRGIYQENLNVRLNNDNIMLVGDGMRYTIITGGRSVKGGYTTY 308

Query: 307 NSATAGIQGPGFMARGIRFVNTAGPRMGQAVALRSSSDLSVFHRCSFEGYQDTLMVLSQR 366
           +SATAGI+G  F+A+GI F NTAGP  GQAVALRSSSDLS+F+RCS EGYQDTLMV SQR
Sbjct: 309 SSATAGIEGLHFIAKGIAFQNTAGPAKGQAVALRSSSDLSIFYRCSIEGYQDTLMVHSQR 368

Query: 367 QFYKQCYVYGTIDFIFGNAAVVLQNCLIYVRRPLKGQVNVITAQGREDPFQNSGISIHNS 426
           QFY++CY+YGT+DFIFGNAAVV QNC+I  R PLKGQ NVITAQGR D FQN+GISIHNS
Sbjct: 369 QFYRECYIYGTVDFIFGNAAVVFQNCIILPRLPLKGQANVITAQGRTDLFQNTGISIHNS 428

Query: 427 QIRAAADLQPMIGSVKTYLGRPWKKYSRTVIMRSYIDSLVSPMGWLAW-QGTRFAQDTLY 486
            I  A DL+P++ SVKTY+GRPW  YSRTV++++YIDS+VSP+GW  W +G+ +  DTL+
Sbjct: 429 IIIPAPDLKPVVRSVKTYMGRPWMMYSRTVVLKTYIDSVVSPVGWSPWTKGSTYGLDTLF 488

Query: 487 YGEYRNFGPRASTRLRVRWPGFHAITTRNVASKFTVRRLIAGHTWLPATGVPFKLDL 535
           Y EY+N GP +STR RVRW GFH ++  + AS F+V + IAG  WLP +G+PF  +L
Sbjct: 489 YAEYKNIGPASSTRWRVRWKGFHVLSKASDASAFSVGKFIAGTAWLPGSGIPFTSEL 540

BLAST of Carg24835 vs. Swiss-Prot
Match: sp|O22149|PME17_ARATH (Probable pectinesterase/pectinesterase inhibitor 17 OS=Arabidopsis thaliana OX=3702 GN=PME17 PE=2 SV=2)

HSP 1 Score: 507.7 bits (1306), Expect = 1.6e-142
Identity = 263/500 (52.60%), Postives = 344/500 (68.80%), Query Frame = 0

Query: 33  INKWCNTTPHPKVCIFFM--SPLKSPPQNRLEFRKMAIKTTLEGAAKAQNYVSRFGPTCK 92
           +  WC+ TP+PK C +F+  +    P ++  EF K+++K  L+ A  A+ +    GP C+
Sbjct: 30  VKAWCSQTPNPKPCEYFLTHNSNNEPIKSESEFLKISMKLVLDRAILAKTHAFTLGPKCR 89

Query: 93  TTRQRAAWTDCFNLYNDTVFQLNRTIQCLDGGNRLRHCTDFDAQTWLSSALTNIDMCSSG 152
            TR++AAW DC  LY+ TV ++N T   +D   +   C+  DAQTWLS+ALTN+D C +G
Sbjct: 90  DTREKAAWEDCIKLYDLTVSKINET---MDPNVK---CSKLDAQTWLSTALTNLDTCRAG 149

Query: 153 AADLNVTDFITPIKCQNVSKMISNCLAINGRFLQEETKSRTMGTEEYQNGFPTWVSSGDR 212
             +L VTD + P+   NVS ++ N LAIN        K     T   ++GFP+WV  GDR
Sbjct: 150 FLELGVTDIVLPLMSNNVSNLLCNTLAIN--------KVPFNYTPPEKDGFPSWVKPGDR 209

Query: 213 KLLQSARVRAHLVVAKDGSGKFRTVQAAINAAARRRGRSRFVIYIKRGVYKENIEVGNDN 272
           KLLQS+  + + VVAKDGSG F+T++ AI+AA+   G  RFVIY+K+GVY EN+E+    
Sbjct: 210 KLLQSSTPKDNAVVAKDGSGNFKTIKEAIDAAS---GSGRFVIYVKQGVYSENLEI--RK 269

Query: 273 ANIMLVGDGIRFTVITSGRSVAAGFTTFNSATAGIQGPGFMARGIRFVNTAGPRMGQAVA 332
            N+ML GDGI  T+IT  +SV  G TTFNSAT    G GF+ARGI F NTAG    QAVA
Sbjct: 270 KNVMLRGDGIGKTIITGSKSVGGGTTTFNSATVAAVGDGFIARGITFRNTAGASNEQAVA 329

Query: 333 LRSSSDLSVFHRCSFEGYQDTLMVLSQRQFYKQCYVYGTIDFIFGNAAVVLQNCLIYVRR 392
           LRS SDLSVF++CSFE YQDTL V S RQFY+ C VYGT+DFIFGNAA VLQNC I+ RR
Sbjct: 330 LRSGSDLSVFYQCSFEAYQDTLYVHSNRQFYRDCDVYGTVDFIFGNAAAVLQNCNIFARR 389

Query: 393 PLKGQVNVITAQGREDPFQNSGISIHNSQIRAAADLQPMIGSVKTYLGRPWKKYSRTVIM 452
           P + + N ITAQGR DP QN+GI IHNS++ AA+DL+P++GS KTYLGRPW++YSRTV M
Sbjct: 390 P-RSKTNTITAQGRSDPNQNTGIIIHNSRVTAASDLRPVLGSTKTYLGRPWRQYSRTVFM 449

Query: 453 RSYIDSLVSPMGWLAWQGTRFAQDTLYYGEYRNFGPRASTRLRVRWPGFHAITTRNVASK 512
           ++ +DSL+ P GWL W G  FA  TL+Y E++N GP AST  RV WPGF  + + + ASK
Sbjct: 450 KTSLDSLIDPRGWLEWDG-NFALKTLFYAEFQNTGPGASTSGRVTWPGFRVLGSASEASK 507

Query: 513 FTVRRLIAGHTWLPATGVPF 531
           FTV   +AG +W+P++ VPF
Sbjct: 510 FTVGTFLAGGSWIPSS-VPF 507

BLAST of Carg24835 vs. Swiss-Prot
Match: sp|O04887|PME2_CITSI (Pectinesterase 2 OS=Citrus sinensis OX=2711 GN=PECS-2.1 PE=2 SV=1)

HSP 1 Score: 501.5 bits (1290), Expect = 1.1e-140
Identity = 265/522 (50.77%), Postives = 350/522 (67.05%), Query Frame = 0

Query: 11  SIALFALLSNSSSSPTKTSGNTINKWCNTTPHPKVCIFFMSPLK--SPPQNRLEFRKMAI 70
           S+ LF+L    S +    S   +  WC  TP+P+ C +F++     +  +   +F K+++
Sbjct: 10  SLVLFSL----SHTSFGYSPEEVKSWCGKTPNPQPCEYFLTQKTDVTSIKQDTDFYKISL 69

Query: 71  KTTLEGAAKAQNYVSRFGPTCKTTRQRAAWTDCFNLYNDTVFQLNRTIQCLDGGNRLRHC 130
           +  LE A  AQ+     G  C+  R++AAW DC  LY  TV +LN+T     G      C
Sbjct: 70  QLALERATTAQSRTYTLGSKCRNEREKAAWEDCRELYELTVLKLNQTSNSSPG------C 129

Query: 131 TDFDAQTWLSSALTNIDMCSSGAADLNVTDFITPIKCQNVSKMISNCLAINGRFLQEETK 190
           T  D QTWLS+ALTN++ C +   DL V +++ P+   NV+K+ISN L++N     E + 
Sbjct: 130 TKVDKQTWLSTALTNLETCRASLEDLGVPEYVLPLLSNNVTKLISNTLSLNKVPYNEPS- 189

Query: 191 SRTMGTEEYQNGFPTWVSSGDRKLLQSARVRAHLVVAKDGSGKFRTVQAAINAAARRRGR 250
                   Y++GFPTWV  GDRKLLQ+   RA++VVA+DGSG  +T+Q A+ AAA R G 
Sbjct: 190 --------YKDGFPTWVKPGDRKLLQTT-PRANIVVAQDGSGNVKTIQEAV-AAASRAGG 249

Query: 251 SRFVIYIKRGVYKENIEVGNDNANIMLVGDGIRFTVITSGRSVAAGFTTFNSATAGIQGP 310
           SR+VIYIK G Y ENIEV     NIM VGDGI  T+IT  +SV  G TTF SAT  + G 
Sbjct: 250 SRYVIYIKAGTYNENIEV--KLKNIMFVGDGIGKTIITGSKSVGGGATTFKSATVAVVGD 309

Query: 311 GFMARGIRFVNTAGPRMGQAVALRSSSDLSVFHRCSFEGYQDTLMVLSQRQFYKQCYVYG 370
            F+AR I   NTAGP   QAVALRS SDLSVF+RCSFEGYQDTL V SQRQFY++C +YG
Sbjct: 310 NFIARDITIRNTAGPNNHQAVALRSGSDLSVFYRCSFEGYQDTLYVHSQRQFYRECDIYG 369

Query: 371 TIDFIFGNAAVVLQNCLIYVRRPLKGQVNVITAQGREDPFQNSGISIHNSQIRAAADLQP 430
           T+DFIFGNAAVVLQNC I+ R+P   + N +TAQGR DP Q++GI IHN ++ AA+DL+P
Sbjct: 370 TVDFIFGNAAVVLQNCNIFARKP-PNRTNTLTAQGRTDPNQSTGIIIHNCRVTAASDLKP 429

Query: 431 MIGSVKTYLGRPWKKYSRTVIMRSYIDSLVSPMGWLAWQGTRFAQDTLYYGEYRNFGPRA 490
           +  SVKT+LGRPWK+YSRTV +++++DSL++P GW+ W G  FA +TLYY EY N GP +
Sbjct: 430 VQSSVKTFLGRPWKQYSRTVYIKTFLDSLINPAGWMEWSGD-FALNTLYYAEYMNTGPGS 489

Query: 491 STRLRVRWPGFHAITTRNVASKFTVRRLIAGHTWLPATGVPF 531
           ST  RV+W G+H +T+ +  S+FTV   IAG++WLPAT VPF
Sbjct: 490 STANRVKWRGYHVLTSPSQVSQFTVGNFIAGNSWLPATNVPF 506

BLAST of Carg24835 vs. TrEMBL
Match: tr|A0A0A0L330|A0A0A0L330_CUCSA (Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_3G088980 PE=3 SV=1)

HSP 1 Score: 855.5 bits (2209), Expect = 6.3e-245
Identity = 429/537 (79.89%), Postives = 468/537 (87.15%), Query Frame = 0

Query: 1   MAPHIQIAFFSIALFALLSNSSSSPTKTSGNTINKWCNTTPHPKVCIFFMSPLKSPPQNR 60
           MA H +I F SI  F LLSN+       S N I KWC TTPHP+VC+  MSP+K PPQNR
Sbjct: 1   MASHYKILFISITFFTLLSNN------VSANPIIKWCKTTPHPRVCVSLMSPIKPPPQNR 60

Query: 61  LEFRKMAIKTTLEGAAKAQNYVSRFGPTCKTTRQRAAWTDCFNLYNDTVFQLNRTIQCL- 120
            EFR+MAI+TTLE AA+A+ Y +RFGPTCKT+RQR AWTDCF LYND V QLNRT+ C+ 
Sbjct: 61  REFRQMAIQTTLEKAAEARAYTARFGPTCKTSRQRTAWTDCFKLYNDVVLQLNRTLHCVV 120

Query: 121 -DGGNRLRHCTDFDAQTWLSSALTNIDMCSSGAADLNVTDFITPIKCQNVSKMISNCLAI 180
            D     R CTDFDAQTWLSSALT+ID+C+SGAADLNVTDFITPIKC NVSKMISNCLAI
Sbjct: 121 TDEAIHRRSCTDFDAQTWLSSALTDIDLCNSGAADLNVTDFITPIKCLNVSKMISNCLAI 180

Query: 181 NGRFLQEETKSRTMGTEEYQNG-FPTWVSSGDRKLLQS--ARVRAHLVVAKDGSGKFRTV 240
           NG FL+EE     +  ++ +NG FP WVS GDRKLL+S   RVRA+LVVAKDGSG FR V
Sbjct: 181 NGGFLEEE----GVKYDDGRNGSFPMWVSEGDRKLLESRPGRVRANLVVAKDGSGTFRRV 240

Query: 241 QAAINAAARRRGRSRFVIYIKRGVYKENIEVGNDNANIMLVGDGIRFTVITSGRSVAAGF 300
           QAAI+AAARRRGR RF+IY+KRGVY+ENIEVGNDN NIMLVGDG+RFTVITSGRSVAAGF
Sbjct: 241 QAAIDAAARRRGRGRFIIYVKRGVYRENIEVGNDNGNIMLVGDGMRFTVITSGRSVAAGF 300

Query: 301 TTFNSATAGIQGPGFMARGIRFVNTAGPRMGQAVALRSSSDLSVFHRCSFEGYQDTLMVL 360
           TTF+SATAGIQGPGF+AR IRFVNTAGPRMGQAVALRSSSDLSVFHRCSFEGYQDTLMVL
Sbjct: 301 TTFSSATAGIQGPGFIARDIRFVNTAGPRMGQAVALRSSSDLSVFHRCSFEGYQDTLMVL 360

Query: 361 SQRQFYKQCYVYGTIDFIFGNAAVVLQNCLIYVRRPLKGQVNVITAQGREDPFQNSGISI 420
           SQRQFYKQCYVYGTIDFIFGNAAVVLQNC+IYVRRPLKGQVNVITAQGREDPFQNSGISI
Sbjct: 361 SQRQFYKQCYVYGTIDFIFGNAAVVLQNCMIYVRRPLKGQVNVITAQGREDPFQNSGISI 420

Query: 421 HNSQIRAAADLQPMIGSVKTYLGRPWKKYSRTVIMRSYIDSLVSPMGWLAWQGTRFAQDT 480
           HNSQIRAAADL+PM+GSVKTYLGRPWKKYSRTVIMRSYID LVSP GWLAWQ ++FAQ T
Sbjct: 421 HNSQIRAAADLRPMVGSVKTYLGRPWKKYSRTVIMRSYIDWLVSPAGWLAWQSSKFAQAT 480

Query: 481 LYYGEYRNFGPRASTRLRVRWPGFHAITTRNVASKFTVRRLIAGHTWLPATGVPFKL 533
           LYYGEYRN GPRASTR RV+WPGFH I + NVASKF+V+RLIAG TWLPATGVPFKL
Sbjct: 481 LYYGEYRNIGPRASTRFRVKWPGFHVIKSPNVASKFSVQRLIAGQTWLPATGVPFKL 527

BLAST of Carg24835 vs. TrEMBL
Match: tr|A0A1S3BUR4|A0A1S3BUR4_CUCME (Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103493398 PE=3 SV=1)

HSP 1 Score: 852.4 bits (2201), Expect = 5.3e-244
Identity = 434/539 (80.52%), Postives = 468/539 (86.83%), Query Frame = 0

Query: 1   MAPHIQ-IAFFSIA-LFALLSNSSSSPTKTSGNTINKWCNTTPHPKVCIFFMSPLKSPPQ 60
           MA H + I F SI  LF +LSN+ S     + N I KWC TTPHPKVCI FMSP+K PPQ
Sbjct: 1   MASHYKIILFISITLLFTILSNNVS-----ANNPIIKWCKTTPHPKVCISFMSPIKPPPQ 60

Query: 61  NRLEFRKMAIKTTLEGAAKAQNYVSRFGPTCKTTRQRAAWTDCFNLYNDTVFQLNRTIQC 120
           NR EFR++AI+TTLE A +A+ Y +RFGPTCKTTRQR AWTDCF LYND VFQLNRT+QC
Sbjct: 61  NREEFRQVAIQTTLEKADEAKAYTARFGPTCKTTRQRTAWTDCFKLYNDVVFQLNRTLQC 120

Query: 121 L---DGGNRLRHCTDFDAQTWLSSALTNIDMCSSGAADLNVTDFITPIKCQNVSKMISNC 180
           L   +  +R   CTDFDAQTWLSSALT+ID+C+SGAADLNVTDFI PIKC NVSKMISNC
Sbjct: 121 LVTDETIHRRPTCTDFDAQTWLSSALTDIDLCNSGAADLNVTDFIAPIKCLNVSKMISNC 180

Query: 181 LAINGRFLQEETKSRTMGTEEYQNGFPTWVSSGDRKLLQS--ARVRAHLVVAKDGSGKFR 240
           LAING FL+EE      G       FP WVS GDRKLL+S   RVRA+LVVAKDGSGKFR
Sbjct: 181 LAINGGFLEEEGVKYDDGG---NGSFPMWVSEGDRKLLESRPRRVRANLVVAKDGSGKFR 240

Query: 241 TVQAAINAAARRRGRSRFVIYIKRGVYKENIEVGNDNANIMLVGDGIRFTVITSGRSVAA 300
            VQAAI+AAARRRGR RFVIY+KRGVY+ENIEVGNDN NIMLVGDG+RFTVITSGRSVAA
Sbjct: 241 RVQAAIDAAARRRGRGRFVIYVKRGVYRENIEVGNDNGNIMLVGDGMRFTVITSGRSVAA 300

Query: 301 GFTTFNSATAGIQGPGFMARGIRFVNTAGPRMGQAVALRSSSDLSVFHRCSFEGYQDTLM 360
           GFTTF+SATAGIQGPGF+AR IRFVNTAGPRMGQAVALRSSSDLSVFHRCSFEGYQDTLM
Sbjct: 301 GFTTFSSATAGIQGPGFIARDIRFVNTAGPRMGQAVALRSSSDLSVFHRCSFEGYQDTLM 360

Query: 361 VLSQRQFYKQCYVYGTIDFIFGNAAVVLQNCLIYVRRPLKGQVNVITAQGREDPFQNSGI 420
           VLSQRQFYKQCY+YGTIDFIFGNAAVVLQNCLIYVRRPLKGQVNVITAQGREDPFQNSGI
Sbjct: 361 VLSQRQFYKQCYIYGTIDFIFGNAAVVLQNCLIYVRRPLKGQVNVITAQGREDPFQNSGI 420

Query: 421 SIHNSQIRAAADLQPMIGSVKTYLGRPWKKYSRTVIMRSYIDSLVSPMGWLAWQGTRFAQ 480
           SIHNSQIRAAADL+PM+GSVKTYLGRPWKKYSRTVIMRSYIDSLVSP GWLAWQ ++FAQ
Sbjct: 421 SIHNSQIRAAADLRPMVGSVKTYLGRPWKKYSRTVIMRSYIDSLVSPAGWLAWQSSKFAQ 480

Query: 481 DTLYYGEYRNFGPRASTRLRVRWPGFHAITTRNVASKFTVRRLIAGHTWLPATGVPFKL 533
            TLYYGEY N GPRASTR RV+WPGFH I + NVASKF+VRRLIAG TWLPATGVPFKL
Sbjct: 481 ATLYYGEYHNIGPRASTRFRVKWPGFHVIKSPNVASKFSVRRLIAGQTWLPATGVPFKL 531

BLAST of Carg24835 vs. TrEMBL
Match: tr|A0A2P4HMV6|A0A2P4HMV6_QUESU (Pectinesterase OS=Quercus suber OX=58331 GN=CFP56_32926 PE=3 SV=1)

HSP 1 Score: 671.8 bits (1732), Expect = 1.3e-189
Identity = 338/526 (64.26%), Postives = 411/526 (78.14%), Query Frame = 0

Query: 10  FSIALFALLSNSSSSPTKTSGNTINKWCNTTPHPKVCIFFM--SPLKSPPQNRLEFRKMA 69
           FS   F  LSN+SS+        I+ WCN TPHP+ C +FM  S     P+NR +FRKM+
Sbjct: 15  FSTTFFTALSNNSSN--------IDWWCNKTPHPEQCKYFMNNSHQDFAPENRSDFRKMS 74

Query: 70  IKTTLEGAAKAQNYVSRFGPTCKTTRQRAAWTDCFNLYNDTVFQLNRTIQCLDGGNRLRH 129
           I+  L+ A  AQ +  +F    +  RQ+AAW+DC  LY  T+ QLNRT+Q   G    R 
Sbjct: 75  IQVALDRAMSAQIHALQFREKIENNRQKAAWSDCLKLYECTILQLNRTLQ---GIGTNRS 134

Query: 130 CTDFDAQTWLSSALTNIDMCSSGAADLNVTDFITPIKC-QNVSKMISNCLAINGRFLQEE 189
           CTDFDAQTWLS+ALTN+D C  G+ +LNV+DFI+PI    NVS++ISN LAING  L EE
Sbjct: 135 CTDFDAQTWLSTALTNLDTCRLGSLELNVSDFISPIMSNNNVSELISNSLAINGVILDEE 194

Query: 190 TKSRTMGTEEYQNGFPTWVSSGDRKLLQ--SARVRAHLVVAKDGSGKFRTVQAAINAAAR 249
           +         Y +GFP+WV+  +RKLLQ  ++ ++A+LVVAKDGSG ++T+QAAINAA+R
Sbjct: 195 S--------NYTHGFPSWVTKHERKLLQASTSSIKANLVVAKDGSGNYKTIQAAINAASR 254

Query: 250 RRGRSRFVIYIKRGVYKENIEVGNDNANIMLVGDGIRFTVITSGRSVAAGFTTFNSATAG 309
           RRG  RFVI++KRGVY ENIEVGN+N NIML+GDG+RFT+ITSGRSV AG TT++SATAG
Sbjct: 255 RRGTGRFVIHVKRGVYFENIEVGNNNNNIMLIGDGMRFTIITSGRSVGAGDTTYSSATAG 314

Query: 310 IQGPGFMARGIRFVNTAGPRMGQAVALRSSSDLSVFHRCSFEGYQDTLMVLSQRQFYKQC 369
           I GP FMARGI F NTAGP  GQAVALRS+SDLSVF+RC+ +GYQDTLMV SQRQFY++C
Sbjct: 315 IDGPHFMARGITFRNTAGPLRGQAVALRSASDLSVFYRCAIQGYQDTLMVHSQRQFYREC 374

Query: 370 YVYGTIDFIFGNAAVVLQNCLIYVRRPLKGQVNVITAQGREDPFQNSGISIHNSQIRAAA 429
           Y+YGTIDFIFGNAAVV QNC+IYVRRPL+GQVNVITAQGR DP+QN+GISI NSQ+RAA 
Sbjct: 375 YIYGTIDFIFGNAAVVFQNCMIYVRRPLRGQVNVITAQGRNDPYQNTGISIQNSQVRAAP 434

Query: 430 DLQPMIGSVKTYLGRPWKKYSRTVIMRSYIDSLVSPMGWLAWQGTRFAQDTLYYGEYRNF 489
           DL+P I S KTYLGRPW +YSRTV + +YIDSLVSP+GWLAW  + FA  TLYYGEY+NF
Sbjct: 435 DLKPYIRSFKTYLGRPWMQYSRTVFLHTYIDSLVSPVGWLAWDRSSFALSTLYYGEYKNF 494

Query: 490 GPRASTRLRVRWPGFHAITTRNVASKFTVRRLIAGHTWLPATGVPF 531
           GPR+STR RVRWPGFH IT+ NVA +F+V+ L+AG +WLPATGVPF
Sbjct: 495 GPRSSTRYRVRWPGFHVITSPNVALRFSVKGLLAGSSWLPATGVPF 521

BLAST of Carg24835 vs. TrEMBL
Match: tr|A0A2N9HXR1|A0A2N9HXR1_FAGSY (Pectinesterase OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS44790 PE=3 SV=1)

HSP 1 Score: 671.0 bits (1730), Expect = 2.2e-189
Identity = 335/524 (63.93%), Postives = 413/524 (78.82%), Query Frame = 0

Query: 12  IALFALLSNSS---SSPTKTSGNTINKWCNTTPHPKVCIFFM--SPLKSPPQNRLEFRKM 71
           I    LLS SS   ++ +K S N ++ WCN TPHP+ C +FM  S     P++R +FRKM
Sbjct: 6   ILFITLLSFSSIFFTAHSKRSSN-LDWWCNKTPHPEPCNYFMRHSRHHFAPKDRSDFRKM 65

Query: 72  AIKTTLEGAAKAQNYVSRFGPTCKTTRQRAAWTDCFNLYNDTVFQLNRTIQCLDGGNRLR 131
            ++  L+    AQ +  +FG   + +RQ+ AW+DC  LY+ TV+ LNRT   L+G    R
Sbjct: 66  VVQVALDRVLSAQRHTMQFGQNLENSRQKLAWSDCSKLYDYTVYHLNRT---LEGLGTNR 125

Query: 132 HCTDFDAQTWLSSALTNIDMCSSGAADLNVTDFITPIKCQNVSKMISNCLAINGRFLQEE 191
            CTDFDAQTWLS+ALTNI+ C  GA +LNV+DFITPI   NVS++ISN LAING  L  E
Sbjct: 126 SCTDFDAQTWLSTALTNIETCRVGALELNVSDFITPITSNNVSELISNSLAINGVILDAE 185

Query: 192 TKSRTMGTEEYQNGFPTWVSSGDRKLLQSARVRAHLVVAKDGSGKFRTVQAAINAAARRR 251
           +         Y +GFP+WV+  +RKLLQ+  ++A+LVVAKDGSG++RTVQAAINAAARRR
Sbjct: 186 S--------NYTDGFPSWVTKHERKLLQATSIKANLVVAKDGSGQYRTVQAAINAAARRR 245

Query: 252 GRSRFVIYIKRGVYKENIEVGNDNANIMLVGDGIRFTVITSGRSVAAGFTTFNSATAGIQ 311
           G  RF+IY+KRG+Y+ENIEVGN+N NIMLVGDG+RFT+ITS RSV AG TT++SATAGI 
Sbjct: 246 GSGRFIIYVKRGIYRENIEVGNNNNNIMLVGDGMRFTIITSSRSVGAGATTYSSATAGID 305

Query: 312 GPGFMARGIRFVNTAGPRMGQAVALRSSSDLSVFHRCSFEGYQDTLMVLSQRQFYKQCYV 371
           G  F+ARGI F NTAGP  GQAVALRS+SDLSVF+RC+ +GYQDTLMV SQRQFY++CY+
Sbjct: 306 GLHFIARGIAFKNTAGPLRGQAVALRSASDLSVFYRCAIQGYQDTLMVHSQRQFYRECYI 365

Query: 372 YGTIDFIFGNAAVVLQNCLIYVRRPLKGQVNVITAQGREDPFQNSGISIHNSQIRAAADL 431
           YGTIDFIFGNAAVV QNCLIYVRRPLKGQVNVITAQGR DP+QN+GI+I NSQ+RAA DL
Sbjct: 366 YGTIDFIFGNAAVVFQNCLIYVRRPLKGQVNVITAQGRNDPYQNTGITIQNSQVRAAPDL 425

Query: 432 QPMIGSVKTYLGRPWKKYSRTVIMRSYIDSLVSPMGWLAWQGTRFAQDTLYYGEYRNFGP 491
           +P++ S KTYLGRPW +YSRTV +++Y+DSL+SP+GWLAW+ + FAQ TLYYGEY+NFGP
Sbjct: 426 KPVVRSFKTYLGRPWMQYSRTVFIQTYLDSLISPVGWLAWERSGFAQRTLYYGEYKNFGP 485

Query: 492 RASTRLRVRWPGFHAITTRNVASKFTVRRLIAGHTWLPATGVPF 531
            +STR RVRWPGFH IT+ NVA +F+V  LIAG +WLPATGVPF
Sbjct: 486 GSSTRYRVRWPGFHVITSPNVALRFSVGGLIAGTSWLPATGVPF 517

BLAST of Carg24835 vs. TrEMBL
Match: tr|A0A2P6SMD4|A0A2P6SMD4_ROSCH (Pectinesterase OS=Rosa chinensis OX=74649 GN=RchiOBHm_Chr1g0374561 PE=3 SV=1)

HSP 1 Score: 651.7 bits (1680), Expect = 1.4e-183
Identity = 322/531 (60.64%), Postives = 407/531 (76.65%), Query Frame = 0

Query: 4   HIQIAFFSIALFALLSNSSSSPTKTSGNTINKWCNTTPHPKVCIFFMS--PLKSPPQNRL 63
           H  + F S+     LS S   P+    N I  WCN TPHP+ C ++MS  P +  P++  
Sbjct: 9   HSLLIFSSMLFQTALSRSHHHPS----NRIRTWCNKTPHPEPCKYWMSHYPHRLTPKHST 68

Query: 64  EFRKMAIKTTLEGAAKAQNYVSRFGPTCKTTRQRAAWTDCFNLYNDTVFQLNRTIQCLDG 123
           +FRK+ ++  LE A  A++Y S+F   C++  Q+AAWTDC  L+++TV QLNRT+Q LD 
Sbjct: 69  DFRKLMVQVALERALSAKSYASQFKQNCESDPQKAAWTDCLKLFDNTVHQLNRTLQGLDT 128

Query: 124 GNRLRHCTDFDAQTWLSSALTNIDMCSSGAADLNVTDFITPI--KCQNVSKMISNCLAIN 183
            ++   CT+FDAQTWLS+ALT I  C  G+ +LNV++FITPI     NVS++ISN LAI 
Sbjct: 129 KHK-ETCTNFDAQTWLSAALTYIHTCRDGSKELNVSNFITPIYTSNNNVSELISNSLAIG 188

Query: 184 GRFLQEETKSRTMGTEEYQNGFPTWVSSGDRKLLQSARVRAHLVVAKDGSGKFRTVQAAI 243
            + L++E ++    T+E    FP+W+S+ DR+LL ++ ++A+LVVAKDGSG FRT+QAA+
Sbjct: 189 SQLLEKENRTT---TDEGYESFPSWLSNHDRRLLAASSIKANLVVAKDGSGHFRTIQAAL 248

Query: 244 NAAARRRGRSRFVIYIKRGVYKENIEVGNDNANIMLVGDGIRFTVITSGRSVAAGFTTFN 303
           N AA+R+  +RFVI++K+GVY+ENIEV N N NIMLVGD +R+T+IT GRSV  G+TTFN
Sbjct: 249 NLAAKRKRMTRFVIHVKKGVYRENIEVVNTNTNIMLVGDSLRYTIITGGRSVKGGYTTFN 308

Query: 304 SATAGIQGPGFMARGIRFVNTAGPRMGQAVALRSSSDLSVFHRCSFEGYQDTLMVLSQRQ 363
           SATAGI GPGF+ARGI F NTAG   GQAVALRSSSDLSVF+RC+F+GYQDTLMV SQRQ
Sbjct: 309 SATAGIDGPGFIARGITFSNTAGALKGQAVALRSSSDLSVFYRCAFQGYQDTLMVHSQRQ 368

Query: 364 FYKQCYVYGTIDFIFGNAAVVLQNCLIYVRRPLKGQVNVITAQGREDPFQNSGISIHNSQ 423
           FY++CY+YGTIDFIFGNAAVV Q+C+IYVR+PL GQVNVITAQ R DPFQ++GISIHNSQ
Sbjct: 369 FYRECYIYGTIDFIFGNAAVVFQSCIIYVRKPLWGQVNVITAQARNDPFQSTGISIHNSQ 428

Query: 424 IRAAADLQPMIGSVKTYLGRPWKKYSRTVIMRSYIDSLVSPMGWLAWQGTRFAQDTLYYG 483
           +RAA DL+P+I   KTYLGRPW +YSR V M+ Y+DSLV P GWL WQ T FA  TLYYG
Sbjct: 429 VRAAPDLKPVIRYYKTYLGRPWMQYSRVVFMKCYLDSLVDPAGWLEWQRTNFALSTLYYG 488

Query: 484 EYRNFGPRASTRLRVRWPGFHAITTRNVASKFTVRRLIAGHTWLPATGVPF 531
           EY+NFGP +STR RV+WPGFH IT+ NVA+ FTV  LIAG +WLP TGVPF
Sbjct: 489 EYKNFGPASSTRYRVKWPGFHVITSANVATSFTVGSLIAGRSWLPTTGVPF 531