CmoCh12G004870 (gene) Cucurbita moschata (Rifu)

Overview
NameCmoCh12G004870
Typegene
OrganismCucurbita moschata (Cucurbita moschata (Rifu))
DescriptionCalcium-binding EF-hand family protein, putative
LocationCmo_Chr12 : 2997928 .. 2998200 (+)
Genome Viewer
Gene Expression
   



Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTAGTAAATATGGCAAGAAGAACTCAAGCTCCATGTTGCCAAGAGAGGAGATAAAAGCAATATTTAAGGAACATGATTTCAATGGCGATGGCCGATTGAGCATCCAAGAATTGTCGAGAGCGTTCGGAATGCTGGGCGCATTTTTACCTCTCTACCGTGCAGTCCGTGGCATCCTCATTGCTGATGTCAATGGAGATGGATATATCACCGAGCATGAACTGGATAAGGTCGTGGATTATGCTATAAAATGTAAGTATAACATAATCTAA

mRNA sequence

ATGGCTAGTAAATATGGCAAGAAGAACTCAAGCTCCATGTTGCCAAGAGAGGAGATAAAAGCAATATTTAAGGAACATGATTTCAATGGCGATGGCCGATTGAGCATCCAAGAATTGTCGAGAGCGTTCGGAATGCTGGGCGCATTTTTACCTCTCTACCGTGCAGTCCGTGGCATCCTCATTGCTGATGTCAATGGAGATGGATATATCACCGAGCATGAACTGGATAAGGTCGTGGATTATGCTATAAAATGTAAGTATAACATAATCTAA

Coding sequence (CDS)

ATGGCTAGTAAATATGGCAAGAAGAACTCAAGCTCCATGTTGCCAAGAGAGGAGATAAAAGCAATATTTAAGGAACATGATTTCAATGGCGATGGCCGATTGAGCATCCAAGAATTGTCGAGAGCGTTCGGAATGCTGGGCGCATTTTTACCTCTCTACCGTGCAGTCCGTGGCATCCTCATTGCTGATGTCAATGGAGATGGATATATCACCGAGCATGAACTGGATAAGGTCGTGGATTATGCTATAAAATGTAAGTATAACATAATCTAA
Homology
[Close]
BLAST of CmoCh12G004870 vs. TrEMBL
Match: A0A0A0LKW4_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G358860 PE=4 SV=1)

HSP 1 Score: 129.0 bits (323), Expect = 2.8e-27
Identity = 59/86 (68.60%), Postives = 71/86 (82.56%), Query Frame = 1 

Query: 5  YGKKNSSSMLPREEIKAIFKEHDFNGDGRLSIQELSRAFGMLGAFLPLYRAVRGILIADV 64
          Y KK S   L +EE+K +F+EHDFNGDGRLSI+ELSRAFG  GAF PLYRA  G+ +AD 
Sbjct: 6  YDKKKSGYKLQKEEMKEMFREHDFNGDGRLSIKELSRAFGQFGAFFPLYRAAFGLFLADD 65

Query: 65 NGDGYITEHELDKVVDYAIKCKYNII 91
          +GDG+I+E ELDKVVDYAIKCKYN++
Sbjct: 66 DGDGFISEQELDKVVDYAIKCKYNLL 91

[Close]
BLAST of CmoCh12G004870 vs. TrEMBL
Match: A0A0A0LRC1_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G358870 PE=4 SV=1)

HSP 1 Score: 93.2 bits (230), Expect = 1.7e-16
Identity = 43/74 (58.11%), Postives = 56/74 (75.68%), Query Frame = 1 

Query: 14 LPREEIKAIFKEHDFNGDGRLSIQELSRAFGMLGAFLPLYRAVRGILIADVNGDGYITEH 73
          L RE+IK IF+EHD +GDGRLS+ E+ +AFG  G+ LP Y+A  G+  AD NGDG+I E 
Sbjct: 10 LSREDIKEIFREHDIDGDGRLSMNEMVKAFGCFGSILPFYKAHYGMAHADENGDGFIEEE 69

Query: 74 ELDKVVDYAIKCKY 88
          ELDK+VDYA K ++
Sbjct: 70 ELDKLVDYAQKFQH 83

[Close]
BLAST of CmoCh12G004870 vs. TrEMBL
Match: A0A0D2QZC2_GOSRA (Uncharacterized protein OS=Gossypium raimondii GN=B456_010G019300 PE=4 SV=1)

HSP 1 Score: 86.7 bits (213), Expect = 1.6e-14
Identity = 40/72 (55.56%), Postives = 54/72 (75.00%), Query Frame = 1 

Query: 18  EIKAIFKEHDFNGDGRLSIQELSRAFGMLGAFLPLYRAVRGILIADVNGDGYITEHELDK 77
           ++KA FK  D N DGRLSI+EL +AF  LGA++P +RA RG+ +AD NGDGY+++ ELD 
Sbjct: 30  QLKAAFKSFDVNKDGRLSIEELRKAFASLGAYIPGWRARRGLSVADGNGDGYVSDDELDD 89

Query: 78  VVDYAIKCKYNI 90
           +V YA+K  Y I
Sbjct: 90  LVKYAMKQGYTI 101

[Close]
BLAST of CmoCh12G004870 vs. TrEMBL
Match: A0A061FBR8_THECC (Calcium-binding EF-hand family protein, putative OS=Theobroma cacao GN=TCM_033854 PE=4 SV=1)

HSP 1 Score: 86.7 bits (213), Expect = 1.6e-14
Identity = 41/84 (48.81%), Postives = 59/84 (70.24%), Query Frame = 1 

Query: 4  KYGKKNSSSMLPREEIKAIFKEHDFNGDGRLSIQELSRAFGMLGAFLPLYRAVRGILIAD 63
          K  +K +  +L   E+++IFK  D+N DGRLS QEL +AF  LG+ LP +RA RG+  AD
Sbjct: 14 KCRRKTTPMLLTGTELRSIFKSFDYNQDGRLSKQELRKAFASLGSHLPGWRAGRGLHHAD 73

Query: 64 VNGDGYITEHELDKVVDYAIKCKY 88
           N DGY+++ ELD +V+YA++C Y
Sbjct: 74 ANRDGYVSDDELDDLVEYALECGY 97

[Close]
BLAST of CmoCh12G004870 vs. TrEMBL
Match: A0A0A0LKX1_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G359940 PE=4 SV=1)

HSP 1 Score: 84.3 bits (207), Expect = 8.0e-14
Identity = 39/67 (58.21%), Postives = 49/67 (73.13%), Query Frame = 1 

Query: 16 REEIKAIFKEHDFNGDGRLSIQELSRAFGMLGAFLPLYRAVRGILIADVNGDGYITEHEL 75
          REE+K IF+EHD +GDG LSI EL +A G LG  +P Y+A  G+   D NGDG I+E EL
Sbjct: 13 REEMKEIFREHDIDGDGYLSISELIKAMGFLGHSIPFYKAHYGMAYCDENGDGLISEDEL 72

Query: 76 DKVVDYA 83
          DK++DYA
Sbjct: 73 DKLIDYA 79

[Close]
BLAST of CmoCh12G004870 vs. TAIR10
Match: AT1G24620.1 (AT1G24620.1 EF hand calcium-binding protein family)

HSP 1 Score: 48.5 bits (114), Expect = 2.5e-06
Identity = 27/72 (37.50%), Postives = 37/72 (51.39%), Query Frame = 1 

Query: 3  SKYGKKNSSSMLPREEIKAIFKEHDFNGDGRLSIQELSRAFGMLGAFLPLYRAVRGILIA 62
          S Y +K  S      E++A+FK+ D NGDG++S +EL      LG  +P     + I   
Sbjct: 22 SPYLQKARSGKTEIRELEAVFKKFDVNGDGKISSKELGAIMTSLGHEVPEEELEKAITEI 81

Query: 63 DVNGDGYITEHE 75
          D  GDGYI   E
Sbjct: 82 DRKGDGYINFEE 93

[Close]
BLAST of CmoCh12G004870 vs. TAIR10
Match: AT1G76650.1 (AT1G76650.1 calmodulin-like 38)

HSP 1 Score: 47.0 bits (110), Expect = 7.2e-06
Identity = 22/62 (35.48%), Postives = 37/62 (59.68%), Query Frame = 1 

Query: 18  EIKAIFKEHDFNGDGRLSIQELSRAFGMLGAFLPLYRAVRGILIADVNGDGYITEHELDK 77
           E++A+F   D N DGR+S +EL ++F  LG  L    AV  + ++D +GDG +   E  +
Sbjct: 43  ELEAVFSYMDANRDGRISPEELQKSFMTLGEQLSDEEAVAAVRLSDTDGDGMLDFEEFSQ 102

Query: 78  VV 80
           ++
Sbjct: 103 LI 104

[Close]
BLAST of CmoCh12G004870 vs. TAIR10
Match: AT3G07490.1 (AT3G07490.1 ARF-GAP domain 11)

HSP 1 Score: 47.0 bits (110), Expect = 7.2e-06
Identity = 23/61 (37.70%), Postives = 37/61 (60.66%), Query Frame = 1 

Query: 14 LPREEIKAIFKEHDFNGDGRLSIQELSRAFGMLGAFLPLYRAVRGILIADVNGDGYITEH 73
          + + E+  IF+  D NGDG+++ QEL+ +   LG ++P    V+ I   D+NGDGY+   
Sbjct: 1  MDQAELARIFQMFDRNGDGKITKQELNDSLENLGIYIPDKDLVQMIEKIDLNGDGYVDIE 60

Query: 74 E 75
          E
Sbjct: 61 E 61

[Close]
BLAST of CmoCh12G004870 vs. NCBI nr
Match: gi|700207396|gb|KGN62515.1| (hypothetical protein Csa_2G358860 [Cucumis sativus])

HSP 1 Score: 129.0 bits (323), Expect = 4.1e-27
Identity = 59/86 (68.60%), Postives = 71/86 (82.56%), Query Frame = 1 

Query: 5  YGKKNSSSMLPREEIKAIFKEHDFNGDGRLSIQELSRAFGMLGAFLPLYRAVRGILIADV 64
          Y KK S   L +EE+K +F+EHDFNGDGRLSI+ELSRAFG  GAF PLYRA  G+ +AD 
Sbjct: 6  YDKKKSGYKLQKEEMKEMFREHDFNGDGRLSIKELSRAFGQFGAFFPLYRAAFGLFLADD 65

Query: 65 NGDGYITEHELDKVVDYAIKCKYNII 91
          +GDG+I+E ELDKVVDYAIKCKYN++
Sbjct: 66 DGDGFISEQELDKVVDYAIKCKYNLL 91

[Close]
BLAST of CmoCh12G004870 vs. NCBI nr
Match: gi|449470198|ref|XP_004152805.1| (PREDICTED: probable calcium-binding protein CML10 [Cucumis sativus])

HSP 1 Score: 117.1 bits (292), Expect = 1.6e-23
Identity = 55/80 (68.75%), Postives = 65/80 (81.25%), Query Frame = 1 

Query: 5  YGKKNSSSMLPREEIKAIFKEHDFNGDGRLSIQELSRAFGMLGAFLPLYRAVRGILIADV 64
          Y KK S   L +EE+K +F+EHDFNGDGRLSI+ELSRAFG  GAF PLYRA  G+ +AD 
Sbjct: 6  YDKKKSGYKLQKEEMKEMFREHDFNGDGRLSIKELSRAFGQFGAFFPLYRAAFGLFLADD 65

Query: 65 NGDGYITEHELDKVVDYAIK 85
          +GDG+I+E ELDKVVDYAIK
Sbjct: 66 DGDGFISEQELDKVVDYAIK 85

[Close]
BLAST of CmoCh12G004870 vs. NCBI nr
Match: gi|700207397|gb|KGN62516.1| (hypothetical protein Csa_2G358870 [Cucumis sativus])

HSP 1 Score: 93.2 bits (230), Expect = 2.5e-16
Identity = 43/74 (58.11%), Postives = 56/74 (75.68%), Query Frame = 1 

Query: 14 LPREEIKAIFKEHDFNGDGRLSIQELSRAFGMLGAFLPLYRAVRGILIADVNGDGYITEH 73
          L RE+IK IF+EHD +GDGRLS+ E+ +AFG  G+ LP Y+A  G+  AD NGDG+I E 
Sbjct: 10 LSREDIKEIFREHDIDGDGRLSMNEMVKAFGCFGSILPFYKAHYGMAHADENGDGFIEEE 69

Query: 74 ELDKVVDYAIKCKY 88
          ELDK+VDYA K ++
Sbjct: 70 ELDKLVDYAQKFQH 83

[Close]
BLAST of CmoCh12G004870 vs. NCBI nr
Match: gi|590592372|ref|XP_007017261.1| (Calcium-binding EF-hand family protein, putative [Theobroma cacao])

HSP 1 Score: 86.7 bits (213), Expect = 2.3e-14
Identity = 41/84 (48.81%), Postives = 59/84 (70.24%), Query Frame = 1 

Query: 4  KYGKKNSSSMLPREEIKAIFKEHDFNGDGRLSIQELSRAFGMLGAFLPLYRAVRGILIAD 63
          K  +K +  +L   E+++IFK  D+N DGRLS QEL +AF  LG+ LP +RA RG+  AD
Sbjct: 14 KCRRKTTPMLLTGTELRSIFKSFDYNQDGRLSKQELRKAFASLGSHLPGWRAGRGLHHAD 73

Query: 64 VNGDGYITEHELDKVVDYAIKCKY 88
           N DGY+++ ELD +V+YA++C Y
Sbjct: 74 ANRDGYVSDDELDDLVEYALECGY 97

[Close]
BLAST of CmoCh12G004870 vs. NCBI nr
Match: gi|823232722|ref|XP_012449049.1| (PREDICTED: polcalcin Che a 3-like [Gossypium raimondii])

HSP 1 Score: 86.7 bits (213), Expect = 2.3e-14
Identity = 40/72 (55.56%), Postives = 54/72 (75.00%), Query Frame = 1 

Query: 18  EIKAIFKEHDFNGDGRLSIQELSRAFGMLGAFLPLYRAVRGILIADVNGDGYITEHELDK 77
           ++KA FK  D N DGRLSI+EL +AF  LGA++P +RA RG+ +AD NGDGY+++ ELD 
Sbjct: 30  QLKAAFKSFDVNKDGRLSIEELRKAFASLGAYIPGWRARRGLSVADGNGDGYVSDDELDD 89

Query: 78  VVDYAIKCKYNI 90
           +V YA+K  Y I
Sbjct: 90  LVKYAMKQGYTI 101

The following BLAST results are available for this feature:
BLAST of CmoCh12G004870 vs. Swiss-Prot
Match NameE-valueIdentityDescription
BLAST of CmoCh12G004870 vs. TrEMBL
Match NameE-valueIdentityDescription
A0A0A0LKW4_CUCSA2.8e-2768.60Uncharacterized protein OS=Cucumis sativus GN=Csa_2G358860 PE=4 SV=1[more]
A0A0A0LRC1_CUCSA1.7e-1658.11Uncharacterized protein OS=Cucumis sativus GN=Csa_2G358870 PE=4 SV=1[more]
A0A0D2QZC2_GOSRA1.6e-1455.56Uncharacterized protein OS=Gossypium raimondii GN=B456_010G019300 PE=4 SV=1[more]
A0A061FBR8_THECC1.6e-1448.81Calcium-binding EF-hand family protein, putative OS=Theobroma cacao GN=TCM_03385... [more]
A0A0A0LKX1_CUCSA8.0e-1458.21Uncharacterized protein OS=Cucumis sativus GN=Csa_2G359940 PE=4 SV=1[more]
BLAST of CmoCh12G004870 vs. TAIR10
Match NameE-valueIdentityDescription
AT1G24620.12.5e-0637.50 EF hand calcium-binding protein family[more]
AT1G76650.17.2e-0635.48 calmodulin-like 38[more]
AT3G07490.17.2e-0637.70 ARF-GAP domain 11[more]
BLAST of CmoCh12G004870 vs. NCBI nr
Match NameE-valueIdentityDescription
gi|700207396|gb|KGN62515.1|4.1e-2768.60hypothetical protein Csa_2G358860 [Cucumis sativus][more]
gi|449470198|ref|XP_004152805.1|1.6e-2368.75PREDICTED: probable calcium-binding protein CML10 [Cucumis sativus][more]
gi|700207397|gb|KGN62516.1|2.5e-1658.11hypothetical protein Csa_2G358870 [Cucumis sativus][more]
gi|590592372|ref|XP_007017261.1|2.3e-1448.81Calcium-binding EF-hand family protein, putative [Theobroma cacao][more]
gi|823232722|ref|XP_012449049.1|2.3e-1455.56PREDICTED: polcalcin Che a 3-like [Gossypium raimondii][more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR002048EF_hand_dom
IPR011992EF-hand-dom_pair
IPR018247EF_Hand_1_Ca_BS
Vocabulary: Molecular Function
TermDefinition
GO:0005509calcium ion binding
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008150 biological_process
cellular_component GO:0005575 cellular_component
molecular_function GO:0005509 calcium ion binding
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmoCh12G004870.1CmoCh12G004870.1mRNA


InterPro
Analysis Name: InterPro Annotations of Cucurbita moschata
Date Performed: 2017-05-19
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002048EF-hand domainPFAMPF13202EF-hand_5coord: 62..79
score: 0.
IPR002048EF-hand domainPFAMPF13405EF-hand_6coord: 18..47
score: 1.
IPR002048EF-hand domainSMARTSM00054efh_1coord: 18..46
score: 1.5E-4coord: 54..82
score:
IPR002048EF-hand domainPROFILEPS50222EF_HAND_2coord: 63..85
score: 7.149coord: 14..49
score: 14
IPR011992EF-hand domain pairGENE3DG3DSA:1.10.238.10coord: 16..78
score: 6.3
IPR011992EF-hand domain pairunknownSSF47473EF-handcoord: 11..76
score: 3.56
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 27..39
score: -coord: 63..75
scor
NoneNo IPR availablePANTHERPTHR10891EF-HAND CALCIUM-BINDING DOMAIN CONTAINING PROTEINcoord: 17..83
score: 8.6
NoneNo IPR availablePANTHERPTHR10891:SF592CALCIUM-BINDING PROTEIN CML38-RELATEDcoord: 17..83
score: 8.6

Synteny
The following gene(s) are orthologous to this gene:
GeneOrthologueOrganismBlock
CmoCh12G004870Cla022567Watermelon (97103) v1cmowmB134
CmoCh12G004870MELO3C010868Melon (DHL92) v3.5.1cmomeB146
CmoCh12G004870ClCG08G015860Watermelon (Charleston Gray)cmowcgB161
CmoCh12G004870Lsi08G014620Bottle gourd (USVL1VR-Ls)cmolsiB175
CmoCh12G004870CsaV3_2G027870Cucumber (Chinese Long) v3cmocucB0186
CmoCh12G004870CsGy4G022320Cucumber (Gy14) v2cgybcmoB449
The following gene(s) are paralogous to this gene:
GeneParalogueOrganismBlock
CmoCh12G004870CmoCh09G002130Cucurbita moschata (Rifu)cmocmoB004
The following block(s) are covering this gene:
GeneOrganismBlock
CmoCh12G004870Cucurbita pepo (Zucchini)cmocpeB154
CmoCh12G004870Cucurbita pepo (Zucchini)cmocpeB166
CmoCh12G004870Cucurbita pepo (Zucchini)cmocpeB172
CmoCh12G004870Cucurbita pepo (Zucchini)cmocpeB174
CmoCh12G004870Bottle gourd (USVL1VR-Ls)cmolsiB167
CmoCh12G004870Cucumber (Gy14) v2cgybcmoB171
CmoCh12G004870Melon (DHL92) v3.6.1cmomedB161
CmoCh12G004870Melon (DHL92) v3.6.1cmomedB188
CmoCh12G004870Silver-seed gourdcarcmoB0108
CmoCh12G004870Silver-seed gourdcarcmoB0257
CmoCh12G004870Silver-seed gourdcarcmoB1042
CmoCh12G004870Cucumber (Chinese Long) v3cmocucB0200
CmoCh12G004870Watermelon (97103) v2cmowmbB178
CmoCh12G004870Watermelon (97103) v2cmowmbB166
CmoCh12G004870Wax gourdcmowgoB0190
CmoCh12G004870Wax gourdcmowgoB0222
CmoCh12G004870Cucurbita moschata (Rifu)cmocmoB141
CmoCh12G004870Cucurbita moschata (Rifu)cmocmoB156
CmoCh12G004870Cucumber (Gy14) v1cgycmoB0641
CmoCh12G004870Cucurbita maxima (Rimu)cmacmoB032
CmoCh12G004870Cucurbita maxima (Rimu)cmacmoB176
CmoCh12G004870Cucurbita maxima (Rimu)cmacmoB453
CmoCh12G004870Cucurbita maxima (Rimu)cmacmoB770
CmoCh12G004870Wild cucumber (PI 183967)cmocpiB160
CmoCh12G004870Wild cucumber (PI 183967)cmocpiB172
CmoCh12G004870Cucumber (Chinese Long) v2cmocuB158
CmoCh12G004870Cucumber (Chinese Long) v2cmocuB169
CmoCh12G004870Melon (DHL92) v3.5.1cmomeB171
CmoCh12G004870Watermelon (Charleston Gray)cmowcgB149
CmoCh12G004870Watermelon (97103) v1cmowmB145