CmoCh08G008450 (gene) Cucurbita moschata (Rifu) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	CmoCh08G008450
Type	gene
Organism	Cucurbita moschata (Cucurbita moschata (Rifu))
Description	(Long-chain-fatty-acid CoA ligase, putative) (6.2.1.3)
Location	Cmo_Chr08 : 5453101 .. 5455193 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CTGTGGCATTTGAATGTTGATGATGGGCGCGGCGATTCCTTTTACTTGCAATCAGCACTTGTGGATTTCTACCATTTCTCGTCATTCGCTCCAAAGTAGCCCTGATTTTCGAATCTCTTGCCAATCGAAGGTAATTTTTGAAATCCGTTTTGAATGTCCTTATGTTATGTTTTAGAGAGAAAATGAATGAATTATAATGTACAGAGAGGAAGGAGGTATTGTCCAATGCTAGAAAGTTGTTTGGTGGAAGGAATTAGTTCCTGATATTTGGAGGTTTTCAGCGGAGAGGTATGGCGATCGGGTAGCATTACTGGATCCATATCATAATCATGCTTCCAAGATTACTTATAAACAGGTATCCATACCATAATCTTGTTAGTATTCCTCGTTTGCTTCTTCAGCATAGTCAGTGATTATGTTTTTGCTTCATTTTAGCAACGAAACACCATCTTGTTTTTTATATTACTTACTTTTCTTTTCTTTTTTTTCTTCATAAATTTAAATTTGCAGCTAGAACAAATTATCTTGAATTTCTGTGAAGGTCTTAGGGCCATTGGAATAAAGCCAGATGAAAAGATTGCACTCTTTTCTGATAATTCATGCCGATGACTTGTTGCCGATCAAGGTATGCGTTGATTGTATAAAATATTGTAGTTCATTTTGTTATATTTTAATAGTTTGGGTTCAGGTATTATGAGTATGGGAGCAATTAATGTTGTTAGAGGTTCAAGATCATCAAGTGAGGAGTTGTTGCAAATATACAATTGAAAGGTTTCTTAAATTGCTTCATAGGTTTTAACATAAACTATGTTTTGGTTTTAAATTTAAACTTTCACTGTGTAGCATTAGTTGTAGACAACCCTGAATTGTTCAATCGAATCATTGAAACATTCCGTTCGAAGGCATCCACGAGATGTATCATTCTTCTATGGGGCGAAAACTCAAGCCTGGCAGCTGAGGGACTGGACGGTATTGGACATCATGGATATGGGATGAGAGAGTCGTAAACTTATGCTTCATAGTAAGTTATATACATATGAAGCTATCAACTTGGATGATATTGCTACTCTTATCTATACAAGTAGCACAACTGGATACCCTAAAGCTGTCATGCTTACCCACAGAAATTTATTGCACCAGGTTTGTTTCACTACTCCTCTTTATTTCACCAAAGTATGATGTGTTCTAAGGCTGCTTGGTATGTCAAACATTTTCCAGATAAAGAATTTATGGGATGTTATACCTGCTAAAGTTGGGGATAAATTTCTAAGCATGCTTTCACCTTGGCATGCGTACGAACGGGCTTGTGAATATTTCATATTTACACTTGGAGTGGAGCAAACGTACACAACAACAAGAAAGTTAAAGGTATTTCAATTACACCAAATCTGGGAAATTAGAATTTTTTTTAAACTCCTCTGTATTTGAGTTGTCAGGATGATCTTCGTGATTATCAACCAGACTACTTGGTTTCTGTTCCATTGGTGTGATTATAGTTCCCAACAAGGGACAACTGTTGTCTGCTACATCCTATCTTAGCAATGAAACCTTGACAAACCTGATTTATAGTGAACTCAGAAAATGGTTAGACTTTTGTGATGTTCCTATGTTCATCATTAGCCATTGACTGTTCGTTCTTAGTCTTTTGTGAAGAAGGAAGGGTGGTTGATAGCAATATAATATGTTGGTTTCATGCAGGACTTCAGAGTGTTCATTTCAAATTAGACCAATCCTCATTGTCAATGAACCATTCACGGTATGAAATTCGTGAAAAAGATACTTCCAATTTGCAAATCTTTTAATTTACTTTTGAACACTTTGAATTGTAGATGGATAATGGGTTACTGAGCCCTACAATGAAAGTTCGAAGACACAACGTTACTGCTTATTACAAGAAGGAAACAGAGAACTTATTCAAGGCAATATAATGTGGTAAGGCAATATAACGTGGTTAATTAGTCTCTGGACTTGGACAAACATTTAGCACAAGCTATATATATAAATAACTTTATAATATGCTACATGTGATACAAGCCTTTATATGATATTCTAGGATTAGTCCATGAACGCAAGTACCAAGATACTATCCTAAG

mRNA sequence

CTGTGGCATTTGAATGTTGATGATGGGCGCGGCGATTCCTTTTACTTGCAATCAGCACTTGTGGATTTCTACCATTTCTCGTCATTCGCTCCAAAGTAGCCCTGATTTTCGAATCTCTTGCCAATCGAAGAGAGGAAGGAGGTATTGTCCAATGCTAGAAACATTAGTTGTAGACAACCCTGAATTGTTCAATCGAATCATTGAAACATTCCGTTCGAAGGCATCCACGAGATGTATCATTCTTCTATGGGGCGAAAACTCAAGCCTGGCAGCTGAGGGACTGGACGCTATCAACTTGGATGATATTGCTACTCTTATCTATACAAGTAGCACAACTGGATACCCTAAAGCTGTCATGCTTACCCACAGAAATTTATTGCACCAGATAAAGAATTTATGGGATGTTATACCTGCTAAAGTTGGGGATAAATTTCTAAGCATGCTTTCACCTTGGCATGCGTACGAACGGGCTTGTGAATATTTCATATTTACACTTGGAGTGGAGCAAACACTACTTGGTTTCTGTTCCATTGGTGTGATTATAGTTCCCAACAAGGGACAACTGTTGTCTGCTACATCCTATCTTAGCAATGAAACCTTGACAAACCTGATTTATAGTGAACTCAGAAAATGGACTTCAGAGTGTTCATTTCAAATTAGACCAATCCTCATTGTCAATGAACCATTCACGATGGATAATGGGTTACTGAGCCCTACAATGAAAGTTCGAAGACACAACGTTACTGCTTATTACAAGAAGGAAACAGAGAACTTATTCAAGGCAATATAATGTGGTAAGGCAATATAACGTGGTTAATTAGTCTCTGGACTTGGACAAACATTTAGCACAAGCTATATATATAAATAACTTTATAATATGCTACATGTGATACAAGCCTTTATATGATATTCTAGGATTAGTCCATGAACGCAAGTACCAAGATACTATCCTAAG

Coding sequence (CDS)

ATGTTGATGATGGGCGCGGCGATTCCTTTTACTTGCAATCAGCACTTGTGGATTTCTACCATTTCTCGTCATTCGCTCCAAAGTAGCCCTGATTTTCGAATCTCTTGCCAATCGAAGAGAGGAAGGAGGTATTGTCCAATGCTAGAAACATTAGTTGTAGACAACCCTGAATTGTTCAATCGAATCATTGAAACATTCCGTTCGAAGGCATCCACGAGATGTATCATTCTTCTATGGGGCGAAAACTCAAGCCTGGCAGCTGAGGGACTGGACGCTATCAACTTGGATGATATTGCTACTCTTATCTATACAAGTAGCACAACTGGATACCCTAAAGCTGTCATGCTTACCCACAGAAATTTATTGCACCAGATAAAGAATTTATGGGATGTTATACCTGCTAAAGTTGGGGATAAATTTCTAAGCATGCTTTCACCTTGGCATGCGTACGAACGGGCTTGTGAATATTTCATATTTACACTTGGAGTGGAGCAAACACTACTTGGTTTCTGTTCCATTGGTGTGATTATAGTTCCCAACAAGGGACAACTGTTGTCTGCTACATCCTATCTTAGCAATGAAACCTTGACAAACCTGATTTATAGTGAACTCAGAAAATGGACTTCAGAGTGTTCATTTCAAATTAGACCAATCCTCATTGTCAATGAACCATTCACGATGGATAATGGGTTACTGAGCCCTACAATGAAAGTTCGAAGACACAACGTTACTGCTTATTACAAGAAGGAAACAGAGAACTTATTCAAGGCAATATAA

Homology

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BLAST of CmoCh08G008450 vs. Swiss-Prot
Match: AAE16_ARATH (Probable acyl-activating enzyme 16, chloroplastic OS=Arabidopsis thaliana GN=AAE16 PE=2 SV=1)

HSP 1 Score: 152.5 bits (384), Expect = 6.2e-36
Identity = 79/147 (53.74%), Postives = 92/147 (62.59%), Query Frame = 1


Query: 51  LVVDNPELFNRIIETFRSKASTRCIILLWGENSSLAAEGLDA------------------ 110
           LVVDNPE FNRI E+F  KA+ + +ILLWGE SSL   G                     
Sbjct: 186 LVVDNPEFFNRIAESFSYKAAPKFVILLWGEKSSLVTAGRHTPVYSYNEIKKFGQERRAK 245

Query: 111 --------------INLDDIATLIYTSSTTGYPKAVMLTHRNLLHQIKNLWDVIPAKVGD 166
                         I+ DDIAT++YTS TTG PK VMLTH+NLLHQI+NL D +PA+ G+
Sbjct: 246 FARSNDSGKYEYEYIDPDDIATIMYTSGTTGNPKGVMLTHQNLLHQIRNLSDFVPAEAGE 305

[Close]

BLAST of CmoCh08G008450 vs. Swiss-Prot
Match: AAE15_ARATH (Long-chain-fatty-acid--[acyl-carrier-protein] ligase AEE15, chloroplastic OS=Arabidopsis thaliana GN=AAE15 PE=1 SV=1)

HSP 1 Score: 136.3 bits (342), Expect = 4.6e-31
Identity = 76/147 (51.70%), Postives = 88/147 (59.86%), Query Frame = 1


Query: 51  LVVDNPELFNRIIETFRSKASTRCIILLWGENSSLAAEGL--------DAINLDDIATLI 110
           +VVDNPE FNRI E+F SKAS R +ILLWGE SSL  +G+        + IN    +   
Sbjct: 202 IVVDNPEFFNRIAESFTSKASLRFLILLWGEKSSLVTQGMQIPVYSYAEIINQGQESRAK 261

Query: 111 YTSS------------------------TTGYPKAVMLTHRNLLHQIKNLWDVIPAKVGD 166
            ++S                        TTG PK VMLTHRNLLHQIK+L   +PA+ GD
Sbjct: 262 LSASNDTRSYRNQFIDSDDTAAIMYTSGTTGNPKGVMLTHRNLLHQIKHLSKYVPAQAGD 321

[Close]

BLAST of CmoCh08G008450 vs. Swiss-Prot
Match: FAC15_MYCMM (Long-chain-fatty-acid--CoA ligase FadD15 OS=Mycobacterium marinum (strain ATCC BAA-535 / M) GN=fadD15 PE=3 SV=1)

HSP 1 Score: 59.3 bits (142), Expect = 7.1e-08
Identity = 35/85 (41.18%), Postives = 44/85 (51.76%), Query Frame = 1


Query: 90  LDAINLDDIATLIYTSSTTGYPKAVMLTHRNLLHQIKNLWDVIPAKV--GDKFLSMLSPW 149
           LDA+   D ATLIYTS TTG PK   LTH NLLH+I+   + +P  +  G + L  L   
Sbjct: 175 LDALRSSDPATLIYTSGTTGRPKGCQLTHSNLLHEIRGTQECLPTLLTPGQRLLVFLPLA 234

Query: 150 HAYERACEYFIFTLGVEQTLLGFCS 173
           H   RA     F     +  +GF S
Sbjct: 235 HVLARALTLSAF---ASKVTVGFTS 256

[Close]

BLAST of CmoCh08G008450 vs. Swiss-Prot
Match: FAD11_MYCTO (Putative fatty-acid--CoA ligase fadD11 OS=Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh) GN=fadD11 PE=3 SV=1)

HSP 1 Score: 54.7 bits (130), Expect = 1.8e-06
Identity = 27/61 (44.26%), Postives = 37/61 (60.66%), Query Frame = 1


Query: 92  AINLDDIATLIYTSSTTGYPKAVMLTHRNLLHQIKNLWDVIPAKVGDKFLSMLSPWHAYE 151
           A+  +DI TLIYTS TTG PK V +TH NLL +   + +V+  + GD+  S L   H  +
Sbjct: 127 AVQPEDIVTLIYTSGTTGNPKGVEMTHANLLFEGYAIDEVLGIRFGDRVTSFLPSAHIAD 186

Query: 152 R 153
           R
Sbjct: 187 R 187

[Close]

BLAST of CmoCh08G008450 vs. Swiss-Prot
Match: FAD11_MYCTU (Putative fatty-acid--CoA ligase fadD11 OS=Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) GN=fadD11 PE=3 SV=1)

HSP 1 Score: 54.7 bits (130), Expect = 1.8e-06
Identity = 27/61 (44.26%), Postives = 37/61 (60.66%), Query Frame = 1


Query: 92  AINLDDIATLIYTSSTTGYPKAVMLTHRNLLHQIKNLWDVIPAKVGDKFLSMLSPWHAYE 151
           A+  +DI TLIYTS TTG PK V +TH NLL +   + +V+  + GD+  S L   H  +
Sbjct: 127 AVQPEDIVTLIYTSGTTGNPKGVEMTHANLLFEGYAIDEVLGIRFGDRVTSFLPSAHIAD 186

Query: 152 R 153
           R
Sbjct: 187 R 187

[Close]

BLAST of CmoCh08G008450 vs. TrEMBL
Match: M5XAK3_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa001870mg PE=4 SV=1)

HSP 1 Score: 175.3 bits (443), Expect = 9.9e-41
Identity = 88/147 (59.86%), Postives = 99/147 (67.35%), Query Frame = 1


Query: 51  LVVDNPELFNRIIETFRSKASTRCIILLWGENSSLAAEGL-------------------- 110
           L VD+PELFNRI E F SK   + +ILLWG+ SSLA+EG                     
Sbjct: 216 LAVDSPELFNRIAEAFSSKVVMKFVILLWGDKSSLASEGKIPVFNYREILDLGRESRKSA 275

Query: 111 ------------DAINLDDIATLIYTSSTTGYPKAVMLTHRNLLHQIKNLWDVIPAKVGD 166
                       +AIN DDIATL+YTS TTG PK VMLTHRNLLHQIKNLWDV+PA+VGD
Sbjct: 276 PDFNDARQQYVHEAINSDDIATLVYTSGTTGNPKGVMLTHRNLLHQIKNLWDVVPAEVGD 335

[Close]

BLAST of CmoCh08G008450 vs. TrEMBL
Match: A0A0L9TMD9_PHAAN (Uncharacterized protein OS=Phaseolus angularis GN=LR48_Vigan01g115700 PE=4 SV=1)

HSP 1 Score: 166.4 bits (420), Expect = 4.6e-38
Identity = 85/146 (58.22%), Postives = 96/146 (65.75%), Query Frame = 1


Query: 51  LVVDNPELFNRIIETFRSKASTRCIILLWGENSSLA------------AEGLD------- 110
           LVVDNPE+FNRI  TF S  + R IILLWGE S L              E +D       
Sbjct: 195 LVVDNPEMFNRIANTFYSSTTMRFIILLWGEKSELVDQESKHVPVFTFTEVIDLGRESGR 254

Query: 111 ------------AINLDDIATLIYTSSTTGYPKAVMLTHRNLLHQIKNLWDVIPAKVGDK 166
                        I+LD IATL+YTS TTG PK VMLTH+NLLHQIKNLWD++PAKVGD+
Sbjct: 255 PLSKAEQRHMNEPIHLDSIATLVYTSGTTGNPKGVMLTHQNLLHQIKNLWDIVPAKVGDR 314

[Close]

BLAST of CmoCh08G008450 vs. TrEMBL
Match: A0A0S3R1K6_PHAAN (Uncharacterized protein OS=Vigna angularis var. angularis GN=Vigan.01G214400 PE=4 SV=1)

HSP 1 Score: 166.4 bits (420), Expect = 4.6e-38
Identity = 85/146 (58.22%), Postives = 96/146 (65.75%), Query Frame = 1


Query: 51  LVVDNPELFNRIIETFRSKASTRCIILLWGENSSLA------------AEGLD------- 110
           LVVDNPE+FNRI  TF S  + R IILLWGE S L              E +D       
Sbjct: 195 LVVDNPEMFNRIANTFYSSTTMRFIILLWGEKSELVDQESKHVPVFTFTEVIDLGRESGR 254

Query: 111 ------------AINLDDIATLIYTSSTTGYPKAVMLTHRNLLHQIKNLWDVIPAKVGDK 166
                        I+LD IATL+YTS TTG PK VMLTH+NLLHQIKNLWD++PAKVGD+
Sbjct: 255 PLSKAEQRHMNEPIHLDSIATLVYTSGTTGNPKGVMLTHQNLLHQIKNLWDIVPAKVGDR 314

[Close]

BLAST of CmoCh08G008450 vs. TrEMBL
Match: V7C657_PHAVU (Uncharacterized protein OS=Phaseolus vulgaris GN=PHAVU_003G051300g PE=4 SV=1)

HSP 1 Score: 164.5 bits (415), Expect = 1.8e-37
Identity = 83/146 (56.85%), Postives = 94/146 (64.38%), Query Frame = 1


Query: 51  LVVDNPELFNRIIETFRSKASTRCIILLWGENSSLAAEG--------------------- 110
           LVVDN E+FNR+  TF S  + R IILLWGE S L A+                      
Sbjct: 195 LVVDNSEMFNRVANTFYSTTTMRFIILLWGEKSELVAQQNKHIPVFTFTQLIDLGAESRT 254

Query: 111 ----------LDAINLDDIATLIYTSSTTGYPKAVMLTHRNLLHQIKNLWDVIPAKVGDK 166
                      +AI  D IATLIYTS TTG PK VMLTH+NLLHQIKNLWD++PAKVGD+
Sbjct: 255 KLSKPEQRYMYEAIKSDSIATLIYTSGTTGNPKGVMLTHQNLLHQIKNLWDIVPAKVGDR 314

[Close]

BLAST of CmoCh08G008450 vs. TrEMBL
Match: U5FIS5_POPTR (Uncharacterized protein (Fragment) OS=Populus trichocarpa GN=POPTR_0017s087001g PE=4 SV=1)

HSP 1 Score: 162.2 bits (409), Expect = 8.7e-37
Identity = 85/149 (57.05%), Postives = 96/149 (64.43%), Query Frame = 1


Query: 51  LVVDNPELFNRIIETFRSKASTRCIILLWGENSSLAA---EGL----------------- 110
           L VDNPELFNRI+ETF SKA+ R +ILLWGE S LA    EG+                 
Sbjct: 211 LAVDNPELFNRIVETFCSKAAPRFVILLWGEKSHLAINRMEGIPIFSYKEIIDLGQESCK 270

Query: 111 --------------DAINLDDIATLIYTSSTTGYPKAVMLTHRNLLHQIKNLWDVIPAKV 166
                         + I+ DDIATL+YTS TTG PK VMLTH+NLLHQI NLWDV+PA+ 
Sbjct: 271 AFSDSDDARQHYKYETISSDDIATLVYTSGTTGNPKGVMLTHKNLLHQINNLWDVVPAQP 330

[Close]

BLAST of CmoCh08G008450 vs. TAIR10
Match: AT3G23790.1 (AT3G23790.1 AMP-dependent synthetase and ligase family protein)

HSP 1 Score: 152.5 bits (384), Expect = 3.5e-37
Identity = 79/147 (53.74%), Postives = 92/147 (62.59%), Query Frame = 1


Query: 51  LVVDNPELFNRIIETFRSKASTRCIILLWGENSSLAAEGLDA------------------ 110
           LVVDNPE FNRI E+F  KA+ + +ILLWGE SSL   G                     
Sbjct: 186 LVVDNPEFFNRIAESFSYKAAPKFVILLWGEKSSLVTAGRHTPVYSYNEIKKFGQERRAK 245

Query: 111 --------------INLDDIATLIYTSSTTGYPKAVMLTHRNLLHQIKNLWDVIPAKVGD 166
                         I+ DDIAT++YTS TTG PK VMLTH+NLLHQI+NL D +PA+ G+
Sbjct: 246 FARSNDSGKYEYEYIDPDDIATIMYTSGTTGNPKGVMLTHQNLLHQIRNLSDFVPAEAGE 305

[Close]

BLAST of CmoCh08G008450 vs. TAIR10
Match: AT4G14070.1 (AT4G14070.1 acyl-activating enzyme 15)

HSP 1 Score: 136.3 bits (342), Expect = 2.6e-32
Identity = 76/147 (51.70%), Postives = 88/147 (59.86%), Query Frame = 1


Query: 51  LVVDNPELFNRIIETFRSKASTRCIILLWGENSSLAAEGL--------DAINLDDIATLI 110
           +VVDNPE FNRI E+F SKAS R +ILLWGE SSL  +G+        + IN    +   
Sbjct: 202 IVVDNPEFFNRIAESFTSKASLRFLILLWGEKSSLVTQGMQIPVYSYAEIINQGQESRAK 261

Query: 111 YTSS------------------------TTGYPKAVMLTHRNLLHQIKNLWDVIPAKVGD 166
            ++S                        TTG PK VMLTHRNLLHQIK+L   +PA+ GD
Sbjct: 262 LSASNDTRSYRNQFIDSDDTAAIMYTSGTTGNPKGVMLTHRNLLHQIKHLSKYVPAQAGD 321

[Close]

BLAST of CmoCh08G008450 vs. TAIR10
Match: AT3G05970.1 (AT3G05970.1 long-chain acyl-CoA synthetase 6)

HSP 1 Score: 53.5 bits (127), Expect = 2.2e-07
Identity = 37/107 (34.58%), Postives = 52/107 (48.60%), Query Frame = 1


Query: 96  DDIATLIYTSSTTGYPKAVMLTHRNLLHQIKNLWDVIPAKVGDKFLSMLSPWHAYERACE 155
           DD+AT+ YTS TTG PK V+LTH NL+  +      +     D ++S L   H YERA +
Sbjct: 261 DDVATICYTSGTTGTPKGVVLTHANLIANVAGSSFSVKFFSSDVYISYLPLAHIYERANQ 320

Query: 156 YFIFTLGVEQTLLGFCSIGVIIVPNKGQLLSATSYLSNETLTNLIYS 203
                 GV    +GF     + + +    L  T + S   L N IY+
Sbjct: 321 ILTVYFGV---AVGFYQGDNMKLLDDLAALRPTVFSSVPRLYNRIYA 364

[Close]

BLAST of CmoCh08G008450 vs. TAIR10
Match: AT5G27600.1 (AT5G27600.1 long-chain acyl-CoA synthetase 7)

HSP 1 Score: 51.6 bits (122), Expect = 8.4e-07
Identity = 38/106 (35.85%), Postives = 51/106 (48.11%), Query Frame = 1


Query: 96  DDIATLIYTSSTTGYPKAVMLTHRNLLHQIKNLWDVIPAKVGDKFLSMLSPWHAYERACE 155
           +DIAT+ YTS TTG PK V+LTH NL+  +            D ++S L   H YERA +
Sbjct: 261 EDIATICYTSGTTGTPKGVVLTHGNLIANVAGSSVEAEFFPSDVYISYLPLAHIYERANQ 320

Query: 156 YFIFTLGVEQTLLGFCSIGVIIVPNKGQLLSATSYLSNETLTNLIY 202
                 GV    +GF    V  + +   +L  T + S   L N IY
Sbjct: 321 IMGVYGGV---AVGFYQGDVFKLMDDFAVLRPTIFCSVPRLYNRIY 363

[Close]

BLAST of CmoCh08G008450 vs. TAIR10
Match: AT1G77590.1 (AT1G77590.1 long chain acyl-CoA synthetase 9)

HSP 1 Score: 51.2 bits (121), Expect = 1.1e-06
Identity = 39/146 (26.71%), Postives = 67/146 (45.89%), Query Frame = 1


Query: 24  HSLQSSPDFRISCQSKRGRRYCPMLETLVVDNPELFNRII---ETFRSKASTRCIILLWG 83
           HSL  +    + C SK  ++   + + L     E   R+I   + F S  ++  +   + 
Sbjct: 172 HSLNETEVTTVICGSKELKKLMDISQQL-----ETVKRVICMDDEFPSDVNSNWMATSFT 231

Query: 84  ENSSLAAEGLDAINLD---DIATLIYTSSTTGYPKAVMLTHRNLLHQIKNLWDVIP-AKV 143
           +   L  E     N     D+A ++YTS +TG PK VM+TH N+L  +  +  ++P    
Sbjct: 232 DVQKLGRENPVDPNFPLSADVAVIMYTSGSTGLPKGVMMTHGNVLATVSAVMTIVPDLGK 291

Query: 144 GDKFLSMLSPWHAYERACEYFIFTLG 163
            D +++ L   H  E A E  + T+G
Sbjct: 292 RDIYMAYLPLAHILELAAESVMATIG 312

[Close]

BLAST of CmoCh08G008450 vs. NCBI nr
Match: gi|596085684|ref|XP_007221302.1| (hypothetical protein PRUPE_ppa001870mg [Prunus persica])

HSP 1 Score: 175.3 bits (443), Expect = 1.4e-40
Identity = 88/147 (59.86%), Postives = 99/147 (67.35%), Query Frame = 1


Query: 51  LVVDNPELFNRIIETFRSKASTRCIILLWGENSSLAAEGL-------------------- 110
           L VD+PELFNRI E F SK   + +ILLWG+ SSLA+EG                     
Sbjct: 216 LAVDSPELFNRIAEAFSSKVVMKFVILLWGDKSSLASEGKIPVFNYREILDLGRESRKSA 275

Query: 111 ------------DAINLDDIATLIYTSSTTGYPKAVMLTHRNLLHQIKNLWDVIPAKVGD 166
                       +AIN DDIATL+YTS TTG PK VMLTHRNLLHQIKNLWDV+PA+VGD
Sbjct: 276 PDFNDARQQYVHEAINSDDIATLVYTSGTTGNPKGVMLTHRNLLHQIKNLWDVVPAEVGD 335

[Close]

BLAST of CmoCh08G008450 vs. NCBI nr
Match: gi|645232166|ref|XP_008222739.1| (PREDICTED: probable acyl-activating enzyme 16, chloroplastic isoform X2 [Prunus mume])

HSP 1 Score: 173.7 bits (439), Expect = 4.1e-40
Identity = 88/147 (59.86%), Postives = 98/147 (66.67%), Query Frame = 1


Query: 51  LVVDNPELFNRIIETFRSKASTRCIILLWGENSSLAAEGL-------------------- 110
           L VD+PELFNRI E F SK   + +ILLWG+ SSLA EG                     
Sbjct: 74  LAVDSPELFNRIAEAFGSKVVMKFVILLWGDKSSLANEGKIPFFNYGEILDLGRESRNSA 133

Query: 111 ------------DAINLDDIATLIYTSSTTGYPKAVMLTHRNLLHQIKNLWDVIPAKVGD 166
                       +AIN DDIATL+YTS TTG PK VMLTHRNLLHQIKNLWDV+PA+VGD
Sbjct: 134 PDFNDARQQYVHEAINSDDIATLVYTSGTTGNPKGVMLTHRNLLHQIKNLWDVVPAEVGD 193

[Close]

BLAST of CmoCh08G008450 vs. NCBI nr
Match: gi|645232163|ref|XP_008222738.1| (PREDICTED: probable acyl-activating enzyme 16, chloroplastic isoform X1 [Prunus mume])

HSP 1 Score: 173.7 bits (439), Expect = 4.1e-40
Identity = 88/147 (59.86%), Postives = 98/147 (66.67%), Query Frame = 1


Query: 51  LVVDNPELFNRIIETFRSKASTRCIILLWGENSSLAAEGL-------------------- 110
           L VD+PELFNRI E F SK   + +ILLWG+ SSLA EG                     
Sbjct: 216 LAVDSPELFNRIAEAFGSKVVMKFVILLWGDKSSLANEGKIPFFNYGEILDLGRESRNSA 275

Query: 111 ------------DAINLDDIATLIYTSSTTGYPKAVMLTHRNLLHQIKNLWDVIPAKVGD 166
                       +AIN DDIATL+YTS TTG PK VMLTHRNLLHQIKNLWDV+PA+VGD
Sbjct: 276 PDFNDARQQYVHEAINSDDIATLVYTSGTTGNPKGVMLTHRNLLHQIKNLWDVVPAEVGD 335

[Close]

BLAST of CmoCh08G008450 vs. NCBI nr
Match: gi|1009175670|ref|XP_015869012.1| (PREDICTED: long-chain-fatty-acid--[acyl-carrier-protein])

HSP 1 Score: 171.4 bits (433), Expect = 2.1e-39
Identity = 88/149 (59.06%), Postives = 97/149 (65.10%), Query Frame = 1


Query: 51  LVVDNPELFNRIIETFRSKASTRCIILLWGENSSLAAEGL-------------------- 110
           LVVD+PELFNRI E FRS A  R +ILLWG  SSL +EG+                    
Sbjct: 81  LVVDSPELFNRISEVFRSNAVMRFVILLWGNKSSLPSEGVEGIPVFSHKEIINLGRESRK 140

Query: 111 --------------DAINLDDIATLIYTSSTTGYPKAVMLTHRNLLHQIKNLWDVIPAKV 166
                         +AIN DDIATL+YTS TTG PK VMLTHRNLLHQI NLWD++PA V
Sbjct: 141 AMFDSNDAGKLYKYEAINSDDIATLVYTSGTTGNPKGVMLTHRNLLHQIHNLWDIVPAAV 200

[Close]

BLAST of CmoCh08G008450 vs. NCBI nr
Match: gi|965658051|dbj|BAT74470.1| (hypothetical protein VIGAN_01214400 [Vigna angularis var. angularis])

HSP 1 Score: 166.4 bits (420), Expect = 6.6e-38
Identity = 85/146 (58.22%), Postives = 96/146 (65.75%), Query Frame = 1


Query: 51  LVVDNPELFNRIIETFRSKASTRCIILLWGENSSLA------------AEGLD------- 110
           LVVDNPE+FNRI  TF S  + R IILLWGE S L              E +D       
Sbjct: 195 LVVDNPEMFNRIANTFYSSTTMRFIILLWGEKSELVDQESKHVPVFTFTEVIDLGRESGR 254

Query: 111 ------------AINLDDIATLIYTSSTTGYPKAVMLTHRNLLHQIKNLWDVIPAKVGDK 166
                        I+LD IATL+YTS TTG PK VMLTH+NLLHQIKNLWD++PAKVGD+
Sbjct: 255 PLSKAEQRHMNEPIHLDSIATLVYTSGTTGNPKGVMLTHQNLLHQIKNLWDIVPAKVGDR 314

The following BLAST results are available for this feature:

BLAST of CmoCh08G008450 vs. Swiss-Prot


Match Name	E-value	Identity	Description
AAE16_ARATH	6.2e-36	53.74	Probable acyl-activating enzyme 16, chloroplastic OS=Arabidopsis thaliana GN=AAE...	[more]
AAE15_ARATH	4.6e-31	51.70	Long-chain-fatty-acid--[acyl-carrier-protein] ligase AEE15, chloroplastic OS=Ara...	[more]
FAC15_MYCMM	7.1e-08	41.18	Long-chain-fatty-acid--CoA ligase FadD15 OS=Mycobacterium marinum (strain ATCC B...	[more]
FAD11_MYCTO	1.8e-06	44.26	Putative fatty-acid--CoA ligase fadD11 OS=Mycobacterium tuberculosis (strain CDC...	[more]
FAD11_MYCTU	1.8e-06	44.26	Putative fatty-acid--CoA ligase fadD11 OS=Mycobacterium tuberculosis (strain ATC...	[more]

BLAST of CmoCh08G008450 vs. TrEMBL


Match Name	E-value	Identity	Description
M5XAK3_PRUPE	9.9e-41	59.86	Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa001870mg PE=4 SV=1	[more]
A0A0L9TMD9_PHAAN	4.6e-38	58.22	Uncharacterized protein OS=Phaseolus angularis GN=LR48_Vigan01g115700 PE=4 SV=1	[more]
A0A0S3R1K6_PHAAN	4.6e-38	58.22	Uncharacterized protein OS=Vigna angularis var. angularis GN=Vigan.01G214400 PE=...	[more]
V7C657_PHAVU	1.8e-37	56.85	Uncharacterized protein OS=Phaseolus vulgaris GN=PHAVU_003G051300g PE=4 SV=1	[more]
U5FIS5_POPTR	8.7e-37	57.05	Uncharacterized protein (Fragment) OS=Populus trichocarpa GN=POPTR_0017s087001g ...	[more]

BLAST of CmoCh08G008450 vs. TAIR10


Match Name	E-value	Identity	Description
AT3G23790.1	3.5e-37	53.74	AMP-dependent synthetase and ligase family protein	[more]
AT4G14070.1	2.6e-32	51.70	acyl-activating enzyme 15	[more]
AT3G05970.1	2.2e-07	34.58	long-chain acyl-CoA synthetase 6	[more]
AT5G27600.1	8.4e-07	35.85	long-chain acyl-CoA synthetase 7	[more]
AT1G77590.1	1.1e-06	26.71	long chain acyl-CoA synthetase 9	[more]

BLAST of CmoCh08G008450 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|596085684\|ref\|XP_007221302.1\|	1.4e-40	59.86	hypothetical protein PRUPE_ppa001870mg [Prunus persica]	[more]
gi\|645232166\|ref\|XP_008222739.1\|	4.1e-40	59.86	PREDICTED: probable acyl-activating enzyme 16, chloroplastic isoform X2 [Prunus ...	[more]
gi\|645232163\|ref\|XP_008222738.1\|	4.1e-40	59.86	PREDICTED: probable acyl-activating enzyme 16, chloroplastic isoform X1 [Prunus ...	[more]
gi\|1009175670\|ref\|XP_015869012.1\|	2.1e-39	59.06	PREDICTED: long-chain-fatty-acid--[acyl-carrier-protein]	[more]
gi\|965658051\|dbj\|BAT74470.1\|	6.6e-38	58.22	hypothetical protein VIGAN_01214400 [Vigna angularis var. angularis]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR000873	AMP-dep_Synth/Lig

Vocabulary: **Molecular Function**
Term	Definition
GO:0003824	catalytic activity

Vocabulary: **Biological Process**
Term	Definition
GO:0008152	metabolic process

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0008152	metabolic process
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0003824	catalytic activity
molecular_function	GO:0016874	ligase activity

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmoCh08G008450.1	CmoCh08G008450.1	mRNA

InterPro

Analysis Name: InterPro Annotations of Cucurbita moschata
Date Performed: 2017-05-19


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000873	AMP-dependent synthetase/ligase	PFAM	PF00501	AMP-binding	coord: 84..166 score: 8.2
None	No IPR available	GENE3D	G3DSA:3.40.50.980		coord: 96..163 score: 2.8
None	No IPR available	PANTHER	PTHR24096	FAMILY NOT NAMED	coord: 50..165 score: 7.3
None	No IPR available	PANTHER	PTHR24096:SF199	LONG-CHAIN-FATTY-ACID--[ACYL-CARRIER-PROTEIN] LIGASE AEE15, CHLOROPLASTIC-RELATED	coord: 50..165 score: 7.3
None	No IPR available	unknown	SSF56801	Acetyl-CoA synthetase-like	coord: 12..163 score: 1.7

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
CmoCh08G008450	Cucsa.135950	Cucumber (Gy14) v1	cgycmoB0377
CmoCh08G008450	CmaCh17G004700	Cucurbita maxima (Rimu)	cmacmoB377
CmoCh08G008450	CmaCh08G008740	Cucurbita maxima (Rimu)	cmacmoB912
CmoCh08G008450	Cla006650	Watermelon (97103) v1	cmowmB869
CmoCh08G008450	Csa6G007970	Cucumber (Chinese Long) v2	cmocuB890
CmoCh08G008450	MELO3C021161	Melon (DHL92) v3.5.1	cmomeB805
CmoCh08G008450	ClCG06G003220	Watermelon (Charleston Gray)	cmowcgB795
CmoCh08G008450	CSPI06G00970	Wild cucumber (PI 183967)	cmocpiB903
CmoCh08G008450	Lsi09G016140	Bottle gourd (USVL1VR-Ls)	cmolsiB803
CmoCh08G008450	Cp4.1LG12g04040	Cucurbita pepo (Zucchini)	cmocpeB840
CmoCh08G008450	Cp4.1LG17g04020	Cucurbita pepo (Zucchini)	cmocpeB853
CmoCh08G008450	MELO3C021161.2	Melon (DHL92) v3.6.1	cmomedB908
CmoCh08G008450	CsaV3_6G001070	Cucumber (Chinese Long) v3	cmocucB1055
CmoCh08G008450	Cla97C06G112610	Watermelon (97103) v2	cmowmbB891
CmoCh08G008450	Bhi12G001879	Wax gourd	cmowgoB1093
CmoCh08G008450	CsGy6G001040	Cucumber (Gy14) v2	cgybcmoB854
CmoCh08G008450	Carg13859	Silver-seed gourd	carcmoB0820
CmoCh08G008450	Carg26698	Silver-seed gourd	carcmoB0760

The following gene(s) are paralogous to this gene:


Gene	Paralogue	Organism	Block
CmoCh08G008450	CmoCh17G004450	Cucurbita moschata (Rifu)	cmocmoB321

The following block(s) are covering this gene:

None