CmaCh08G008740 (gene) Cucurbita maxima (Rimu)

Overview
NameCmaCh08G008740
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu))
Description4-coumarate--CoA ligase-like 5
LocationCma_Chr08 : 5404921 .. 5406982 (+)
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TATTCCTCTGTTCTACTTCAATATTCTTATGCTCTGTGGCATTTGAATGTTGATGATGGGCGCGGCGATTTCTACCATTTCTCGTCATTCGCTCCAAAGTAGCCCTGATTTTCGAATCTCTTGCCAATCGAAGGTAATTTTTGAAATCCCTTTTGAATGTCATTATGTTATGATTTAGAGAGAAAATGAATGAATTATAATGTAGAGAGACAGGTCGGAGTTAAGGAGGTATTGTCCAATGCTAGAAAGTTGTTTGGTGTTAGGTATTAGTTCCTGATATTTGGAGGTCTTCAGCGGAGAGGTATGGCGATCGGGTAGTATTACTGGATCCATACCATAATCCTGCTTCCAAAATTACTTATAAACAGGTATCCATACCATAATCTTGTTAGTATTCCTCGTTTGCTTCTTCAGCATAGTCCGTGATTATGTTTTTGCTTCATTTTAGCAACGAAACACCATCTTGTTTCTTATATTACTTACTTTTTTTTTTTTCTTCATAAATTTAAATTTGCAGCTAGAACAAAGTATCTTGAATTTCTGTGAAGGTCTTAGGGCCATTGGAATAAAGCCAGATGAAAAGATTGCACTCTTTTCTGATAATTCATGCCGATTGGCTTGTTGCCGATCAAGGTATGCGTTGATTGTATAAAATATTGTATTTCATTTTGTTATATTTTAATAATTTGGGTTCAAGTATTATGAGTATGGGAGCAATTAATGTTGTTAGAGGTTCAAGATCATCAAGTGAGGAGTTGTTGCAAATATACAATCACAATGAAAGGTTTCTTAAATTGCTTCATATTTACTGTATGTTACAGAGAATCAAAGGTTTTAATATAAACTATGTTTTGGTTTTAAATTTAAACTTTCCCAGTGTAGCATTAGTTGTAGACAACCCTGAATTGTTCAATCGAATAGTTGAAACATTCCGTTCGAAGGCATCCACGAGATGTATCATTCTTCTATGGGGCGAAAACTCAAGCCTGGCAGCTGAGGGACTGGATGGTATTACTTTCTTTGATTATAATGACATCATGGATATGGGATGAGAGTCGTAAACTTATGTTTCATAGTAAGTTATATACATATGAAGCTATCAACTTGGATGATATTGCTACTCTTATCTATACAAGTGGCACAACTGGAAGCCCTAAAGCTGTCATGCTTACCCACAGAAATTTCTTGCACCAGGTTTGTTTCATTACTCTCTTTATTTCACCAACGTATGATGTGTTCTAAGGCTGCTTTGTATGTCAAACATTTTCCAGATAAAGAATTTATGGGATGCTATACCTGCTAAAGTTGGGGATAAATTTCTAAGCATGCTTCCACCTTGGCATGCGTACGAACGGGCTTGTGAATATTTCATATTTACACATGGAGTGGAGCAAACGTACACAACGACAAGAAAGTTAAAGGTATTTCAAACTCCTCTGTATTTGAATTGTCAGGATGATCTTCGTGATTATCAACCAGACTACTTGGTTTCTGTTCCATTGGTGTGATTATAGTTCCCAACAAGGGACAACTGTTGTCTGCTACATCCGATCTTAGCAATGAAACCTTGACAAGCCTGATTTATAGTGAACTCATAAAATGGTTAGACTTTTGTGATGTTCCTATGTTCATCATTAGCCATTGACTGTTCGTTCTTAGTCTTTTGTGAAGAAGGAAGGGTGGTTGATAGCAATATAATATGTTGGTTTAATTCATGCAGGACCTCAGAATGTTCATTTCAAATTGGACCAATCCTCATTGTCAATGAACCATTCACAGTATGAAATTCGTGAATGTAATATAAAAAGATAATGGGTTACTAAGCCCTACAATGGAAATTCGAAGAGACAACGTTACAAGAAGGAAATTGAGAACTTATTCAAGGCAATATAATGTGGATAATTAGTCTCTGGACTTGGACAAACATTTAGCACAAGCTATATATATAAATGACTTTATATGTGATTCAAGGCCATGAATGTAAGTACCAACTTACTATCCTAAGGCCATGAACTTACGCCCCATGTATTTTTACGATAGGTAGGTGCTATTCCAACCCATG

mRNA sequence

TATTCCTCTGTTCTACTTCAATATTCTTATGCTCTGTGGCATTTGAATGTTGATGATGGGCGCGGCGATTTCTACCATTTCTCGTCATTCGCTCCAAAGTAGCCCTGATTTTCGAATCTCTTGCCAATCGAAGGTATTAGTTCCTGATATTTGGAGGTCTTCAGCGGAGAGGTATGGCGATCGGGTAGTATTACTGGATCCATACCATAATCCTGCTTCCAAAATTACTTATAAACAGCTAGAACAAAGTATCTTGAATTTCTGTGAAGGTCTTAGGGCCATTGGAATAAAGCCAGATGAAAAGATTGCACTCTTTTCTGATAATTCATGCCGATTGGCTTGTTGCCGATCAAGTATTATGAGTATGGGAGCAATTAATGTTGTTAGAGGTTCAAGATCATCAAGTGAGGAGTTGTTGCAAATATACAATCACAATGAAAGTGTAGCATTAGTTGTAGACAACCCTGAATTGTTCAATCGAATAGTTGAAACATTCCGTTCGAAGGCATCCACGAGATGTATCATTCTTCTATGGGGCGAAAACTCAAGCCTGGCAGCTGAGGGACTGGATGCTATCAACTTGGATGATATTGCTACTCTTATCTATACAAGTGGCACAACTGGAAGCCCTAAAGCTGTCATGCTTACCCACAGAAATTTCTTGCACCAGATAAAGAATTTATGGGATGCTATACCTGCTAAAGTTGGGGATAAATTTCTAAGCATGCTTCCACCTTGGCATGCGTACGAACGGGCTTGTGAATATTTCATATTTACACATGGAGTGGAGCAAACGTACACAACGACAAGAAAGTTAAAGGTATTTCAAACTCCTCTGTATTTGAATTGTCAGGATGATCTTCGTGATTATCAACCAGACTACTTGGTTTCTGTTCCATTGGTGTGATTATAGTTCCCAACAAGGGACAACTGTTGTCTGCTACATCCGATCTTAGCAATGAAACCTTGACAAGCCTGATTTATAGTGAACTCATAAAATGGACCTCAGAATGTTCATTTCAAATTGGACCAATCCTCATTGTCAATGAACCATTCACAGTATGAAATTCGTGAATGTAATATAAAAAGATAATGGGTTACTAAGCCCTACAATGGAAATTCGAAGAGACAACGTTACAAGAAGGAAATTGAGAACTTATTCAAGGCAATATAATGTGGATAATTAGTCTCTGGACTTGGACAAACATTTAGCACAAGCTATATATATAAATGACTTTATATGTGATTCAAGGCCATGAATGTAAGTACCAACTTACTATCCTAAGGCCATGAACTTACGCCCCATGTATTTTTACGATAGGTAGGTGCTATTCCAACCCATG

Coding sequence (CDS)

ATGTTGATGATGGGCGCGGCGATTTCTACCATTTCTCGTCATTCGCTCCAAAGTAGCCCTGATTTTCGAATCTCTTGCCAATCGAAGGTATTAGTTCCTGATATTTGGAGGTCTTCAGCGGAGAGGTATGGCGATCGGGTAGTATTACTGGATCCATACCATAATCCTGCTTCCAAAATTACTTATAAACAGCTAGAACAAAGTATCTTGAATTTCTGTGAAGGTCTTAGGGCCATTGGAATAAAGCCAGATGAAAAGATTGCACTCTTTTCTGATAATTCATGCCGATTGGCTTGTTGCCGATCAAGTATTATGAGTATGGGAGCAATTAATGTTGTTAGAGGTTCAAGATCATCAAGTGAGGAGTTGTTGCAAATATACAATCACAATGAAAGTGTAGCATTAGTTGTAGACAACCCTGAATTGTTCAATCGAATAGTTGAAACATTCCGTTCGAAGGCATCCACGAGATGTATCATTCTTCTATGGGGCGAAAACTCAAGCCTGGCAGCTGAGGGACTGGATGCTATCAACTTGGATGATATTGCTACTCTTATCTATACAAGTGGCACAACTGGAAGCCCTAAAGCTGTCATGCTTACCCACAGAAATTTCTTGCACCAGATAAAGAATTTATGGGATGCTATACCTGCTAAAGTTGGGGATAAATTTCTAAGCATGCTTCCACCTTGGCATGCGTACGAACGGGCTTGTGAATATTTCATATTTACACATGGAGTGGAGCAAACGTACACAACGACAAGAAAGTTAAAGGTATTTCAAACTCCTCTGTATTTGAATTGTCAGGATGATCTTCGTGATTATCAACCAGACTACTTGGTTTCTGTTCCATTGGTGTGA

Protein sequence

MLMMGAAISTISRHSLQSSPDFRISCQSKVLVPDIWRSSAERYGDRVVLLDPYHNPASKITYKQLEQSILNFCEGLRAIGIKPDEKIALFSDNSCRLACCRSSIMSMGAINVVRGSRSSSEELLQIYNHNESVALVVDNPELFNRIVETFRSKASTRCIILLWGENSSLAAEGLDAINLDDIATLIYTSGTTGSPKAVMLTHRNFLHQIKNLWDAIPAKVGDKFLSMLPPWHAYERACEYFIFTHGVEQTYTTTRKLKVFQTPLYLNCQDDLRDYQPDYLVSVPLV
Homology
[Close]
BLAST of CmaCh08G008740 vs. Swiss-Prot
Match: AAE15_ARATH (Long-chain-fatty-acid--[acyl-carrier-protein] ligase AEE15, chloroplastic OS=Arabidopsis thaliana GN=AAE15 PE=1 SV=1)

HSP 1 Score: 318.5 bits (815), Expect = 7.2e-86
Identity = 170/287 (59.23%), Postives = 198/287 (68.99%), Query Frame = 1 

Query: 32  VPDIWRSSAERYGDRVVLLDPYHNPASKITYKQLEQSILNFCEGLRAIGIKPDEKIALFS 91
           VPDIWRSSAE+YGDRV L+DPYH+P  K+TYKQLEQ IL+F EGLR +G+K DEKIALF+
Sbjct: 99  VPDIWRSSAEKYGDRVALVDPYHDPPLKLTYKQLEQEILDFAEGLRVLGVKADEKIALFA 158

Query: 92  DNSCRLACCRSSIMSMGAINVVRGSRSSSEELLQIYNHNESVALVVDNPELFNRIVETFR 151
           DNSCR       IM+ GA+NVVRGSRSS EELLQIY H+ESVA+VVDNPE FNRI E+F 
Sbjct: 159 DNSCRWLVSDQGIMATGAVNVVRGSRSSVEELLQIYRHSESVAIVVDNPEFFNRIAESFT 218

Query: 152 SKASTRCIILLWGENSSLAAEGL--------DAIN--LDDIATLIYTSGT---------- 211
           SKAS R +ILLWGE SSL  +G+        + IN   +  A L  ++ T          
Sbjct: 219 SKASLRFLILLWGEKSSLVTQGMQIPVYSYAEIINQGQESRAKLSASNDTRSYRNQFIDS 278

Query: 212 ------------TGSPKAVMLTHRNFLHQIKNLWDAIPAKVGDKFLSMLPPWHAYERACE 271
                       TG+PK VMLTHRN LHQIK+L   +PA+ GDKFLSMLP WHAYERA E
Sbjct: 279 DDTAAIMYTSGTTGNPKGVMLTHRNLLHQIKHLSKYVPAQAGDKFLSMLPSWHAYERASE 338

Query: 272 YFIFTHGVEQTYTTTRKLKVFQTPLYLNCQDDLRDYQPDYLVSVPLV 287
           YFIFT GVEQ YT+ R LK           DDL+ YQP+Y+VSVPLV
Sbjct: 339 YFIFTCGVEQMYTSIRYLK-----------DDLKRYQPNYIVSVPLV 374

[Close]
BLAST of CmaCh08G008740 vs. Swiss-Prot
Match: AAE16_ARATH (Probable acyl-activating enzyme 16, chloroplastic OS=Arabidopsis thaliana GN=AAE16 PE=2 SV=1)

HSP 1 Score: 311.2 bits (796), Expect = 1.2e-83
Identity = 163/287 (56.79%), Postives = 190/287 (66.20%), Query Frame = 1 

Query: 32  VPDIWRSSAERYGDRVVLLDPYHNPASKITYKQLEQSILNFCEGLRAIGIKPDEKIALFS 91
           VPDIWRSS E+YGDRV ++DPYH+P S  TY+QLEQ IL+F EGLR +G+K DEKIALF+
Sbjct: 83  VPDIWRSSVEKYGDRVAVVDPYHDPPSTFTYRQLEQEILDFVEGLRVVGVKADEKIALFA 142

Query: 92  DNSCRLACCRSSIMSMGAINVVRGSRSSSEELLQIYNHNESVALVVDNPELFNRIVETFR 151
           DNSCR       IM+ GA+NVVRGSRSS EELLQIY H+ESVALVVDNPE FNRI E+F 
Sbjct: 143 DNSCRWLVADQGIMATGAVNVVRGSRSSVEELLQIYCHSESVALVVDNPEFFNRIAESFS 202

Query: 152 SKASTRCIILLWGENSSLAAEGLDA-------------------INLDDIATLIYTS--- 211
            KA+ + +ILLWGE SSL   G                         +D     Y     
Sbjct: 203 YKAAPKFVILLWGEKSSLVTAGRHTPVYSYNEIKKFGQERRAKFARSNDSGKYEYEYIDP 262

Query: 212 ----------GTTGSPKAVMLTHRNFLHQIKNLWDAIPAKVGDKFLSMLPPWHAYERACE 271
                     GTTG+PK VMLTH+N LHQI+NL D +PA+ G++FLSMLP WHAYERACE
Sbjct: 263 DDIATIMYTSGTTGNPKGVMLTHQNLLHQIRNLSDFVPAEAGERFLSMLPSWHAYERACE 322

Query: 272 YFIFTHGVEQTYTTTRKLKVFQTPLYLNCQDDLRDYQPDYLVSVPLV 287
           YFIFT GVEQ YT+ R LK           DDL+ YQP YL+SVPLV
Sbjct: 323 YFIFTCGVEQKYTSIRFLK-----------DDLKRYQPHYLISVPLV 358

[Close]
BLAST of CmaCh08G008740 vs. Swiss-Prot
Match: FAC15_MYCMM (Long-chain-fatty-acid--CoA ligase FadD15 OS=Mycobacterium marinum (strain ATCC BAA-535 / M) GN=fadD15 PE=3 SV=1)

HSP 1 Score: 93.2 bits (230), Expect = 4.9e-18
Identity = 71/244 (29.10%), Postives = 114/244 (46.72%), Query Frame = 1 

Query: 60  ITYKQLEQSILNFCEGLRAIGIKPDEKIALFSDNSCRLACCRSSIMSMGAINVVRGSRSS 119
           IT  +  + I +   GL A+G++  +++++FS      A    +I+++GA+ V     SS
Sbjct: 46  ITCAEAARHIRSAALGLIALGVQAGDRVSIFSATCYEWAILDLAILAVGAVTVPIYETSS 105

Query: 120 SEELLQIYNHNESVALVVDNPELFNRIVETFRSKASTRCIILLWGEN----SSLAAEG-- 179
           +E++  +  ++E+V    +       I E      + R ++++ G        LA EG  
Sbjct: 106 AEQVRWVLQNSEAVLAFAETDAHAAMIAELTGDLPALRRVLVINGSGPKALEQLAEEGGS 165

Query: 180 ---------LDAINLDDIATLIYTSGTTGSPKAVMLTHRNFLHQIKNLWDAIPAKV--GD 239
                    LDA+   D ATLIYTSGTTG PK   LTH N LH+I+   + +P  +  G 
Sbjct: 166 VDRAELTARLDALRSSDPATLIYTSGTTGRPKGCQLTHSNLLHEIRGTQECLPTLLTPGQ 225

Query: 240 KFLSMLPPWHAYERACEYFIFTHGVEQTYTTTRKLKVFQTPLYLNCQDDLRDYQPDYLVS 287
           + L  LP  H   RA     F   V   +T+  K  +   PL+         ++P  +VS
Sbjct: 226 RLLVFLPLAHVLARALTLSAFASKVTVGFTSDIKNLL---PLF-------AVFKPTVVVS 279

[Close]
BLAST of CmaCh08G008740 vs. Swiss-Prot
Match: MENE_BACWK (2-succinylbenzoate--CoA ligase OS=Bacillus weihenstephanensis (strain KBAB4) GN=menE PE=3 SV=1)

HSP 1 Score: 78.2 bits (191), Expect = 1.6e-13
Identity = 49/174 (28.16%), Postives = 85/174 (48.85%), Query Frame = 1 

Query: 59  KITYKQLEQSILNFCEGLRAIGIKPDEKIALFSDNSCRLACCRSSIMSMGAINVVRGSRS 118
           K+T+ QL + +++ CE L  +G+K  +K+A+   N   +     ++  +GA+ V+  +R 
Sbjct: 27  KVTFVQLHEKVVSVCEHLTHVGVKRAQKVAVLMKNGMEMITVIHALSYIGAVAVLLNTRL 86

Query: 119 SSEELLQIYNHNESVALVVDNPELFNRIVETFRSKASTRCIILLWGENSSLAAEGLDAIN 178
           S EELL   +  E V LV D         + F ++    C      +     A   +  +
Sbjct: 87  SREELLWQMDDAEVVCLVTD---------QEFETENVPVCSFAEVMQGPKAEAFIQEEFS 146

Query: 179 LDDIATLIYTSGTTGSPKAVMLTHRNFLHQIKNLWDAIPAKVGDKFLSMLPPWH 233
           L++  T+IYTSGTTG PK V+LT+ N           +  +  D +L+ +P +H
Sbjct: 147 LEEAMTIIYTSGTTGKPKGVILTYGNHWASAVGSSLNLGLRDDDCWLACMPMFH 191

[Close]
BLAST of CmaCh08G008740 vs. Swiss-Prot
Match: ACSL1_MOUSE (Long-chain-fatty-acid--CoA ligase 1 OS=Mus musculus GN=Acsl1 PE=1 SV=2)

HSP 1 Score: 76.6 bits (187), Expect = 4.8e-13
Identity = 68/262 (25.95%), Postives = 106/262 (40.46%), Query Frame = 1 

Query: 54  HNPASKITYKQLEQSILNFCEGLRAIGIKP--DEKIALFSDNSCRLACCRSSIMSMGAIN 113
           + P   I+YK++ +       GL   G KP  ++ I LFS N            S   + 
Sbjct: 116 NQPYEWISYKEVAELAECIGSGLIQKGFKPCSEQFIGLFSQNRPEWVIVEQGCFSYSMVV 175

Query: 114 VVRGSRSSSEELLQIYNHNESVALVVDNPELFNRIVETFRSKAST--RCIILLWGENSSL 173
           V       ++ +  I N  E   +  D PE    ++E   +K +   + I+++    S L
Sbjct: 176 VPLYDTLGADAITYIVNKAELSVIFADKPEKAKLLLEGVENKLTPCLKIIVIMDSYGSDL 235

Query: 174 AAEG----LDAINL-------------------DDIATLIYTSGTTGSPKAVMLTHRNFL 233
              G    ++ I+L                   +D+A + +TSGTTG+PK  M+TH+N +
Sbjct: 236 VERGKKCGVEIISLKALEDLGRVNRVKPKPPEPEDLAIICFTSGTTGNPKGAMITHQNII 295

Query: 234 HQ----IKNLWDAIPAKVGDKFLSMLPPWHAYERACEYFIFTHGVEQTYTTTRKLKVFQT 285
           +     IK    A  A   D  +S LP  H +E   E  +  HG         K+  FQ 
Sbjct: 296 NDCSGFIKATESAFIASTDDVLISFLPLAHMFETVVECVMLCHGA--------KIGFFQG 355

[Close]
BLAST of CmaCh08G008740 vs. TrEMBL
Match: A0A022QUF6_ERYGU (Uncharacterized protein OS=Erythranthe guttata GN=MIMGU_mgv1a001985mg PE=4 SV=1)

HSP 1 Score: 360.1 bits (923), Expect = 2.4e-96
Identity = 178/290 (61.38%), Postives = 210/290 (72.41%), Query Frame = 1 

Query: 32  VPDIWRSSAERYGDRVVLLDPYHNPASKITYKQLEQSILNFCEGLRAIGIKPDEKIALFS 91
           +PDIWRSSA+++GDRV L+DPYH PA+ +TYKQLEQ ILNFCEGLR IG+KP+EK+ALF+
Sbjct: 89  IPDIWRSSAQKFGDRVALVDPYHKPATNMTYKQLEQEILNFCEGLRVIGLKPEEKLALFA 148

Query: 92  DNSCRLACCRSSIMSMGAINVVRGSRSSSEELLQIYNHNESVALVVDNPELFNRIVETFR 151
           DNSCR       IM+ GAINVVRG+RSSSEELLQIYNH+ESVALVVDNPE++NRIVETF+
Sbjct: 149 DNSCRWLVSDQGIMATGAINVVRGTRSSSEELLQIYNHSESVALVVDNPEMYNRIVETFQ 208

Query: 152 SKASTRCIILLWGENSS---------------------------LAAEGLDA-------- 211
           S+A  R ++LLWGE SS                           L     DA        
Sbjct: 209 SRAIVRFVVLLWGEKSSIPNGESATDIPIYSYEEIIKFGHENRALLLRSEDARNQYIYKT 268

Query: 212 INLDDIATLIYTSGTTGSPKAVMLTHRNFLHQIKNLWDAIPAKVGDKFLSMLPPWHAYER 271
           I+ DD+ATL+YTSGTTG+PK VMLTHRN LHQ+ NLWD +PA  GD+FLSMLPPWHAYER
Sbjct: 269 ISSDDVATLVYTSGTTGNPKGVMLTHRNLLHQVSNLWDIVPAVPGDRFLSMLPPWHAYER 328

Query: 272 ACEYFIFTHGVEQTYTTTRKLKVFQTPLYLNCQDDLRDYQPDYLVSVPLV 287
           ACEYFIFTHGVE  YT+ +KL+           DDLR YQP Y++SVPLV
Sbjct: 329 ACEYFIFTHGVEHVYTSVQKLR-----------DDLRRYQPHYVISVPLV 367

[Close]
BLAST of CmaCh08G008740 vs. TrEMBL
Match: V7C657_PHAVU (Uncharacterized protein OS=Phaseolus vulgaris GN=PHAVU_003G051300g PE=4 SV=1)

HSP 1 Score: 351.3 bits (900), Expect = 1.1e-93
Identity = 177/287 (61.67%), Postives = 203/287 (70.73%), Query Frame = 1 

Query: 31  LVPDIWRSSAERYGDRVVLLDPYHNPASKITYKQLEQSILNFCEGLRAIGIKPDEKIALF 90
           +VPDIWRSSAE+YGD V L+DPYH+P + +TYKQLEQ+IL+F EGLR IG+KPDEK+ALF
Sbjct: 91  VVPDIWRSSAEKYGDNVALVDPYHHPPTTMTYKQLEQAILDFAEGLRVIGVKPDEKLALF 150

Query: 91  SDNSCRLACCRSSIMSMGAINVVRGSRSSSEELLQIYNHNESVALVVDNPELFNRIVETF 150
           +DNSCR       +M+ GAINVVRGSRSS  ELLQIYNH+ESVALVVDN E+FNR+  TF
Sbjct: 151 ADNSCRWLVADQGMMASGAINVVRGSRSSVAELLQIYNHSESVALVVDNSEMFNRVANTF 210

Query: 151 RSKASTRCIILLWGENSSLAAEG-------------------------------LDAINL 210
            S  + R IILLWGE S L A+                                 +AI  
Sbjct: 211 YSTTTMRFIILLWGEKSELVAQQNKHIPVFTFTQLIDLGAESRTKLSKPEQRYMYEAIKS 270

Query: 211 DDIATLIYTSGTTGSPKAVMLTHRNFLHQIKNLWDAIPAKVGDKFLSMLPPWHAYERACE 270
           D IATLIYTSGTTG+PK VMLTH+N LHQIKNLWD +PAKVGD+FLSMLPPWHAYERACE
Sbjct: 271 DSIATLIYTSGTTGNPKGVMLTHQNLLHQIKNLWDIVPAKVGDRFLSMLPPWHAYERACE 330

Query: 271 YFIFTHGVEQTYTTTRKLKVFQTPLYLNCQDDLRDYQPDYLVSVPLV 287
           YFIFT G++Q YTT R LK           DDL  YQP YL+SVPLV
Sbjct: 331 YFIFTCGIQQQYTTVRNLK-----------DDLERYQPHYLISVPLV 366

[Close]
BLAST of CmaCh08G008740 vs. TrEMBL
Match: A0A078EGI0_BRANA (BnaA04g06290D protein OS=Brassica napus GN=BnaA04g06290D PE=4 SV=1)

HSP 1 Score: 349.7 bits (896), Expect = 3.3e-93
Identity = 181/307 (58.96%), Postives = 210/307 (68.40%), Query Frame = 1 

Query: 5   GAAISTISRHSLQSSPDFRISCQSKVLVPDIWRSSAERYGDRVVLLDPYHNPASKITYKQ 64
           G  ++ I   S  S  D  +       VPDIWRSSA++YGD+V L+DPYH+P  K+TY Q
Sbjct: 68  GKEVTPIIESSSLSGGDAALRSNEWKAVPDIWRSSADKYGDKVALVDPYHDPPLKLTYNQ 127

Query: 65  LEQSILNFCEGLRAIGIKPDEKIALFSDNSCRLACCRSSIMSMGAINVVRGSRSSSEELL 124
           LEQ IL+F EGLRA+G+KPDEKIALF+DNSCR       IMS GA+NVVRGSRSS EELL
Sbjct: 128 LEQEILDFAEGLRAVGVKPDEKIALFADNSCRWLVSDQGIMSTGAVNVVRGSRSSVEELL 187

Query: 125 QIYNHNESVALVVDNPELFNRIVETFRSKASTRCIILLWGENSSLAAEGLD--------- 184
           QIY H+ESVALVVDNPE FNRI +TF SKAS R +ILLWGE SSL  +G+          
Sbjct: 188 QIYQHSESVALVVDNPEFFNRIADTFTSKASLRFLILLWGEKSSLVTQGMQIPVYSYTDI 247

Query: 185 ----------------AINLDDIATLIYTSGTTGSPKAVMLTHRNFLHQIKNLWDAIPAK 244
                            IN DD A ++YTSGTTG+PK VMLTHRN LHQIK+L   +PA+
Sbjct: 248 KNLGQEKRAGSNDTRKPINPDDTAAIMYTSGTTGNPKGVMLTHRNLLHQIKHLSAYVPAE 307

Query: 245 VGDKFLSMLPPWHAYERACEYFIFTHGVEQTYTTTRKLKVFQTPLYLNCQDDLRDYQPDY 287
            GD+FLSMLP WHAYERACEYFIFT GVEQ YT+ R LK           DDL+ YQP Y
Sbjct: 308 AGDRFLSMLPSWHAYERACEYFIFTCGVEQMYTSIRFLK-----------DDLKRYQPHY 363

[Close]
BLAST of CmaCh08G008740 vs. TrEMBL
Match: M4EVC4_BRARP (Uncharacterized protein OS=Brassica rapa subsp. pekinensis PE=4 SV=1)

HSP 1 Score: 349.4 bits (895), Expect = 4.3e-93
Identity = 181/307 (58.96%), Postives = 210/307 (68.40%), Query Frame = 1 

Query: 5   GAAISTISRHSLQSSPDFRISCQSKVLVPDIWRSSAERYGDRVVLLDPYHNPASKITYKQ 64
           G  ++ I   S  S  D  +       VPDIWRSSA++YGD+V L+DPYH+P  K+TY Q
Sbjct: 68  GKEVTPIIESSSLSGGDAALRSNEWKAVPDIWRSSADKYGDKVALVDPYHDPPLKLTYNQ 127

Query: 65  LEQSILNFCEGLRAIGIKPDEKIALFSDNSCRLACCRSSIMSMGAINVVRGSRSSSEELL 124
           LEQ IL+F EGLRA+G+KPDEKIALF+DNSCR       IMS GA+NVVRGSRSS EELL
Sbjct: 128 LEQEILDFSEGLRAVGVKPDEKIALFADNSCRWLVSDQGIMSTGAVNVVRGSRSSVEELL 187

Query: 125 QIYNHNESVALVVDNPELFNRIVETFRSKASTRCIILLWGENSSLAAEGLD--------- 184
           QIY H+ESVALVVDNPE FNRI +TF SKAS R +ILLWGE SSL  +G+          
Sbjct: 188 QIYQHSESVALVVDNPEFFNRIADTFTSKASLRFLILLWGEKSSLVTQGMQIPVYSYTDI 247

Query: 185 ----------------AINLDDIATLIYTSGTTGSPKAVMLTHRNFLHQIKNLWDAIPAK 244
                            IN DD A ++YTSGTTG+PK VMLTHRN LHQIK+L   +PA+
Sbjct: 248 KNLGQEKRAGSNDTRKPINPDDTAAIMYTSGTTGNPKGVMLTHRNLLHQIKHLSAYVPAE 307

Query: 245 VGDKFLSMLPPWHAYERACEYFIFTHGVEQTYTTTRKLKVFQTPLYLNCQDDLRDYQPDY 287
            GD+FLSMLP WHAYERACEYFIFT GVEQ YT+ R LK           DDL+ YQP Y
Sbjct: 308 AGDRFLSMLPSWHAYERACEYFIFTCGVEQMYTSIRFLK-----------DDLKRYQPHY 363

[Close]
BLAST of CmaCh08G008740 vs. TrEMBL
Match: A0A0D3BYS9_BRAOL (Uncharacterized protein OS=Brassica oleracea var. oleracea GN=AAE15 PE=4 SV=1)

HSP 1 Score: 348.2 bits (892), Expect = 9.5e-93
Identity = 179/307 (58.31%), Postives = 210/307 (68.40%), Query Frame = 1 

Query: 5   GAAISTISRHSLQSSPDFRISCQSKVLVPDIWRSSAERYGDRVVLLDPYHNPASKITYKQ 64
           G  ++ I   S  S  D  +S      VPDIWRSSAE+YGD++ L+DPYH+P  K+TY Q
Sbjct: 74  GKDVTPIIESSSSSGGDAALSYSEWKAVPDIWRSSAEKYGDKIALVDPYHDPPLKLTYNQ 133

Query: 65  LEQSILNFCEGLRAIGIKPDEKIALFSDNSCRLACCRSSIMSMGAINVVRGSRSSSEELL 124
           LEQ IL+F EGLRA+G+K DEKIALF+DNSCR       +MS GA+NVVRGSRSS EELL
Sbjct: 134 LEQEILDFAEGLRAVGVKADEKIALFADNSCRWLVSDQGVMSTGAVNVVRGSRSSVEELL 193

Query: 125 QIYNHNESVALVVDNPELFNRIVETFRSKASTRCIILLWGENSSLAAEGLD--------- 184
           QIY H+ESVALVVDNPE FNRI +TF SK S R +ILLWGE SSL  +G+          
Sbjct: 194 QIYQHSESVALVVDNPEFFNRIADTFTSKVSLRFLILLWGEKSSLVTQGMQIPVYSYTDI 253

Query: 185 ----------------AINLDDIATLIYTSGTTGSPKAVMLTHRNFLHQIKNLWDAIPAK 244
                           +IN DD A ++YTSGTTG+PK VMLTHRN LHQIK+L   +PA+
Sbjct: 254 KNLGQEKRAGSNDTRKSINPDDTAAIMYTSGTTGNPKGVMLTHRNLLHQIKHLSAYVPAE 313

Query: 245 VGDKFLSMLPPWHAYERACEYFIFTHGVEQTYTTTRKLKVFQTPLYLNCQDDLRDYQPDY 287
            GD+FLSMLP WHAYERACEYFIFT GVEQ YT+ R LK           DDL+ YQP Y
Sbjct: 314 AGDRFLSMLPSWHAYERACEYFIFTCGVEQMYTSIRFLK-----------DDLKRYQPHY 369

[Close]
BLAST of CmaCh08G008740 vs. TAIR10
Match: AT4G14070.1 (AT4G14070.1 acyl-activating enzyme 15)

HSP 1 Score: 318.5 bits (815), Expect = 4.1e-87
Identity = 170/287 (59.23%), Postives = 198/287 (68.99%), Query Frame = 1 

Query: 32  VPDIWRSSAERYGDRVVLLDPYHNPASKITYKQLEQSILNFCEGLRAIGIKPDEKIALFS 91
           VPDIWRSSAE+YGDRV L+DPYH+P  K+TYKQLEQ IL+F EGLR +G+K DEKIALF+
Sbjct: 99  VPDIWRSSAEKYGDRVALVDPYHDPPLKLTYKQLEQEILDFAEGLRVLGVKADEKIALFA 158

Query: 92  DNSCRLACCRSSIMSMGAINVVRGSRSSSEELLQIYNHNESVALVVDNPELFNRIVETFR 151
           DNSCR       IM+ GA+NVVRGSRSS EELLQIY H+ESVA+VVDNPE FNRI E+F 
Sbjct: 159 DNSCRWLVSDQGIMATGAVNVVRGSRSSVEELLQIYRHSESVAIVVDNPEFFNRIAESFT 218

Query: 152 SKASTRCIILLWGENSSLAAEGL--------DAIN--LDDIATLIYTSGT---------- 211
           SKAS R +ILLWGE SSL  +G+        + IN   +  A L  ++ T          
Sbjct: 219 SKASLRFLILLWGEKSSLVTQGMQIPVYSYAEIINQGQESRAKLSASNDTRSYRNQFIDS 278

Query: 212 ------------TGSPKAVMLTHRNFLHQIKNLWDAIPAKVGDKFLSMLPPWHAYERACE 271
                       TG+PK VMLTHRN LHQIK+L   +PA+ GDKFLSMLP WHAYERA E
Sbjct: 279 DDTAAIMYTSGTTGNPKGVMLTHRNLLHQIKHLSKYVPAQAGDKFLSMLPSWHAYERASE 338

Query: 272 YFIFTHGVEQTYTTTRKLKVFQTPLYLNCQDDLRDYQPDYLVSVPLV 287
           YFIFT GVEQ YT+ R LK           DDL+ YQP+Y+VSVPLV
Sbjct: 339 YFIFTCGVEQMYTSIRYLK-----------DDLKRYQPNYIVSVPLV 374

[Close]
BLAST of CmaCh08G008740 vs. TAIR10
Match: AT3G23790.1 (AT3G23790.1 AMP-dependent synthetase and ligase family protein)

HSP 1 Score: 311.2 bits (796), Expect = 6.5e-85
Identity = 163/287 (56.79%), Postives = 190/287 (66.20%), Query Frame = 1 

Query: 32  VPDIWRSSAERYGDRVVLLDPYHNPASKITYKQLEQSILNFCEGLRAIGIKPDEKIALFS 91
           VPDIWRSS E+YGDRV ++DPYH+P S  TY+QLEQ IL+F EGLR +G+K DEKIALF+
Sbjct: 83  VPDIWRSSVEKYGDRVAVVDPYHDPPSTFTYRQLEQEILDFVEGLRVVGVKADEKIALFA 142

Query: 92  DNSCRLACCRSSIMSMGAINVVRGSRSSSEELLQIYNHNESVALVVDNPELFNRIVETFR 151
           DNSCR       IM+ GA+NVVRGSRSS EELLQIY H+ESVALVVDNPE FNRI E+F 
Sbjct: 143 DNSCRWLVADQGIMATGAVNVVRGSRSSVEELLQIYCHSESVALVVDNPEFFNRIAESFS 202

Query: 152 SKASTRCIILLWGENSSLAAEGLDA-------------------INLDDIATLIYTS--- 211
            KA+ + +ILLWGE SSL   G                         +D     Y     
Sbjct: 203 YKAAPKFVILLWGEKSSLVTAGRHTPVYSYNEIKKFGQERRAKFARSNDSGKYEYEYIDP 262

Query: 212 ----------GTTGSPKAVMLTHRNFLHQIKNLWDAIPAKVGDKFLSMLPPWHAYERACE 271
                     GTTG+PK VMLTH+N LHQI+NL D +PA+ G++FLSMLP WHAYERACE
Sbjct: 263 DDIATIMYTSGTTGNPKGVMLTHQNLLHQIRNLSDFVPAEAGERFLSMLPSWHAYERACE 322

Query: 272 YFIFTHGVEQTYTTTRKLKVFQTPLYLNCQDDLRDYQPDYLVSVPLV 287
           YFIFT GVEQ YT+ R LK           DDL+ YQP YL+SVPLV
Sbjct: 323 YFIFTCGVEQKYTSIRFLK-----------DDLKRYQPHYLISVPLV 358

[Close]
BLAST of CmaCh08G008740 vs. TAIR10
Match: AT2G04350.1 (AT2G04350.1 AMP-dependent synthetase and ligase family protein)

HSP 1 Score: 73.2 bits (178), Expect = 3.0e-13
Identity = 59/258 (22.87%), Postives = 112/258 (43.41%), Query Frame = 1 

Query: 61  TYKQLEQSILNFCEGLRAIGIKPDEKIALFSDNSCR-----LACCRSSIMSMGAINVVRG 120
           +Y ++ + + NF  GL  +G   D+++A+FSD           C R S      I VV  
Sbjct: 131 SYGEVFERVCNFASGLVNVGHNVDDRVAIFSDTRAEWFIAFQGCFRQS------ITVVTI 190

Query: 121 SRSSSEELLQIYNHNES-VALVVDNPELFNRIVETFRSKASTRCIILLWGENSSLAAEGL 180
             S  EE L IY+ NE+ V+ ++ + +   ++     S  + + II +  +   +A+  +
Sbjct: 191 YASLGEEAL-IYSLNETRVSTLICDSKQLKKLSAIQSSLKTVKNIIYIEEDGVDVASSDV 250

Query: 181 DAI-------------------------NLDDIATLIYTSGTTGSPKAVMLTHRNFLHQI 240
           +++                         + + +A +++TSG+TG PK VM+TH N +   
Sbjct: 251 NSMGDITVSSISEVEKLGQKNAVQPILPSKNGVAVIMFTSGSTGLPKGVMITHGNLVATA 310

Query: 241 KNLWDAIP-AKVGDKFLSMLPPWHAYERACEYFIFTHGVEQTYTTTRKLKVFQTPLYLNC 287
             +   +P     D +++ LP  H +E   E  +FT G    Y +   L      +    
Sbjct: 311 AGVMKVVPKLDKNDTYIAYLPLAHVFELEAEIVVFTSGSAIGYGSAMTLTDTSNKVKKGT 370

[Close]
BLAST of CmaCh08G008740 vs. TAIR10
Match: AT1G77590.1 (AT1G77590.1 long chain acyl-CoA synthetase 9)

HSP 1 Score: 68.9 bits (167), Expect = 5.6e-12
Identity = 57/247 (23.08%), Postives = 102/247 (41.30%), Query Frame = 1 

Query: 60  ITYKQLEQSILNFCEGLRAIGIKPDEKIALFSDNSCRLACCRSSIMSMGAINVVRGSRSS 119
           +T+ +  +++ +F  GL  IG K +E++A+F+D                   V   S   
Sbjct: 107 LTFGKTLEAVCDFASGLVQIGHKTEERVAIFADTREEWFISLQGCFRRNVTVVTIYSSLG 166

Query: 120 SEELLQIYNHNESVALVVDNPELFNRIVETFRSKASTRCIILLWGENSS------LAAEG 179
            E L    N  E   ++  + EL  ++++  +   + + +I +  E  S      +A   
Sbjct: 167 EEALCHSLNETEVTTVICGSKEL-KKLMDISQQLETVKRVICMDDEFPSDVNSNWMATSF 226

Query: 180 LDAINLD-------------DIATLIYTSGTTGSPKAVMLTHRNFLHQIKNLWDAIP-AK 239
            D   L              D+A ++YTSG+TG PK VM+TH N L  +  +   +P   
Sbjct: 227 TDVQKLGRENPVDPNFPLSADVAVIMYTSGSTGLPKGVMMTHGNVLATVSAVMTIVPDLG 286

Query: 240 VGDKFLSMLPPWHAYERACEYFIFTHGVEQTYTTTRKLKVFQTPLYLNCQDDLRDYQPDY 287
             D +++ LP  H  E A E  + T G    Y +   L      +    + D+   +P  
Sbjct: 287 KRDIYMAYLPLAHILELAAESVMATIGSAIGYGSPLTLTDTSNKIKKGTKGDVTALKPTI 346

[Close]
BLAST of CmaCh08G008740 vs. TAIR10
Match: AT4G11030.1 (AT4G11030.1 AMP-dependent synthetase and ligase family protein)

HSP 1 Score: 67.0 bits (162), Expect = 2.1e-11
Identity = 68/249 (27.31%), Postives = 110/249 (44.18%), Query Frame = 1 

Query: 34  DIWRSSAERYGDRVVL--LDPYHNPASKI---TYKQLEQSILNFCEGLRAIGIKPDEKIA 93
           DI+R++ E+Y +  +L   +  +  A K    TYK++   ++     LR+ GIK  EK  
Sbjct: 49  DIFRTAVEKYPNNRMLGRREISNGKAGKYVWKTYKEVYDIVIKLGNSLRSCGIKEGEKCG 108

Query: 94  LFSDNSCRLACCRSSIMSMGAINVVRGSRSSSEELLQIYNHNE-SVALVVDN--PELFN- 153
           ++  N C       +  + G   V       +  +  I +H E S+A V +   PELF  
Sbjct: 109 IYGINCCEWIISMEACNAHGLYCVPLYDTLGAGAVEFIISHAEVSIAFVEEKKIPELFKT 168

Query: 154 -----RIVETFRSKASTR-----------CIILLWGENSSLAAEG----LDAINLDDIAT 213
                + ++T  S    +            +I  W E   L  EG    L      DI T
Sbjct: 169 CPNSTKYMKTVVSFGGVKPEQKEEAEKLGLVIHSWDEFLKLG-EGKQYELPIKKPSDICT 228

Query: 214 LIYTSGTTGSPKAVMLTHRNF-------LHQIKNLWDAIPAKVGDKFLSMLPPWHAYERA 247
           ++YTSGTTG PK VM+++ +        +H + N+  ++  K  D ++S LP  H ++RA
Sbjct: 229 IMYTSGTTGDPKGVMISNESIVTITTGVMHFLGNVNASLSEK--DVYISYLPLAHVFDRA 288

[Close]
BLAST of CmaCh08G008740 vs. NCBI nr
Match: gi|645232163|ref|XP_008222738.1| (PREDICTED: probable acyl-activating enzyme 16, chloroplastic isoform X1 [Prunus mume])

HSP 1 Score: 365.2 bits (936), Expect = 1.1e-97
Identity = 183/287 (63.76%), Postives = 208/287 (72.47%), Query Frame = 1 

Query: 32  VPDIWRSSAERYGDRVVLLDPYHNPASKITYKQLEQSILNFCEGLRAIGIKPDEKIALFS 91
           VPDIWRSSAERYGDRV L DPYH+P S +TYKQLE+ IL+F EGLR +G+KP+EKIALF+
Sbjct: 113 VPDIWRSSAERYGDRVALTDPYHDPPSSMTYKQLEEGILDFSEGLRVVGVKPEEKIALFA 172

Query: 92  DNSCRLACCRSSIMSMGAINVVRGSRSSSEELLQIYNHNESVALVVDNPELFNRIVETFR 151
           DNSCR       IM+ GAINVVRGSRSS EELLQIY+H+ESVAL VD+PELFNRI E F 
Sbjct: 173 DNSCRWLVADQGIMATGAINVVRGSRSSVEELLQIYDHSESVALAVDSPELFNRIAEAFG 232

Query: 152 SKASTRCIILLWGENSSLAAEGL--------------------------------DAINL 211
           SK   + +ILLWG+ SSLA EG                                 +AIN 
Sbjct: 233 SKVVMKFVILLWGDKSSLANEGKIPFFNYGEILDLGRESRNSAPDFNDARQQYVHEAINS 292

Query: 212 DDIATLIYTSGTTGSPKAVMLTHRNFLHQIKNLWDAIPAKVGDKFLSMLPPWHAYERACE 271
           DDIATL+YTSGTTG+PK VMLTHRN LHQIKNLWD +PA+VGD+FLSMLPPWHAYERACE
Sbjct: 293 DDIATLVYTSGTTGNPKGVMLTHRNLLHQIKNLWDVVPAEVGDRFLSMLPPWHAYERACE 352

Query: 272 YFIFTHGVEQTYTTTRKLKVFQTPLYLNCQDDLRDYQPDYLVSVPLV 287
           YFIFT G+EQ YTT R LK           DDLR YQP+Y++SVPLV
Sbjct: 353 YFIFTCGIEQVYTTVRNLK-----------DDLRHYQPNYIISVPLV 388

[Close]
BLAST of CmaCh08G008740 vs. NCBI nr
Match: gi|848888612|ref|XP_012844494.1| (PREDICTED: probable acyl-activating enzyme 16, chloroplastic [Erythranthe guttata])

HSP 1 Score: 360.1 bits (923), Expect = 3.5e-96
Identity = 178/290 (61.38%), Postives = 210/290 (72.41%), Query Frame = 1 

Query: 32  VPDIWRSSAERYGDRVVLLDPYHNPASKITYKQLEQSILNFCEGLRAIGIKPDEKIALFS 91
           +PDIWRSSA+++GDRV L+DPYH PA+ +TYKQLEQ ILNFCEGLR IG+KP+EK+ALF+
Sbjct: 89  IPDIWRSSAQKFGDRVALVDPYHKPATNMTYKQLEQEILNFCEGLRVIGLKPEEKLALFA 148

Query: 92  DNSCRLACCRSSIMSMGAINVVRGSRSSSEELLQIYNHNESVALVVDNPELFNRIVETFR 151
           DNSCR       IM+ GAINVVRG+RSSSEELLQIYNH+ESVALVVDNPE++NRIVETF+
Sbjct: 149 DNSCRWLVSDQGIMATGAINVVRGTRSSSEELLQIYNHSESVALVVDNPEMYNRIVETFQ 208

Query: 152 SKASTRCIILLWGENSS---------------------------LAAEGLDA-------- 211
           S+A  R ++LLWGE SS                           L     DA        
Sbjct: 209 SRAIVRFVVLLWGEKSSIPNGESATDIPIYSYEEIIKFGHENRALLLRSEDARNQYIYKT 268

Query: 212 INLDDIATLIYTSGTTGSPKAVMLTHRNFLHQIKNLWDAIPAKVGDKFLSMLPPWHAYER 271
           I+ DD+ATL+YTSGTTG+PK VMLTHRN LHQ+ NLWD +PA  GD+FLSMLPPWHAYER
Sbjct: 269 ISSDDVATLVYTSGTTGNPKGVMLTHRNLLHQVSNLWDIVPAVPGDRFLSMLPPWHAYER 328

Query: 272 ACEYFIFTHGVEQTYTTTRKLKVFQTPLYLNCQDDLRDYQPDYLVSVPLV 287
           ACEYFIFTHGVE  YT+ +KL+           DDLR YQP Y++SVPLV
Sbjct: 329 ACEYFIFTHGVEHVYTSVQKLR-----------DDLRRYQPHYVISVPLV 367

[Close]
BLAST of CmaCh08G008740 vs. NCBI nr
Match: gi|593781169|ref|XP_007153625.1| (hypothetical protein PHAVU_003G051300g [Phaseolus vulgaris])

HSP 1 Score: 351.3 bits (900), Expect = 1.6e-93
Identity = 177/287 (61.67%), Postives = 203/287 (70.73%), Query Frame = 1 

Query: 31  LVPDIWRSSAERYGDRVVLLDPYHNPASKITYKQLEQSILNFCEGLRAIGIKPDEKIALF 90
           +VPDIWRSSAE+YGD V L+DPYH+P + +TYKQLEQ+IL+F EGLR IG+KPDEK+ALF
Sbjct: 91  VVPDIWRSSAEKYGDNVALVDPYHHPPTTMTYKQLEQAILDFAEGLRVIGVKPDEKLALF 150

Query: 91  SDNSCRLACCRSSIMSMGAINVVRGSRSSSEELLQIYNHNESVALVVDNPELFNRIVETF 150
           +DNSCR       +M+ GAINVVRGSRSS  ELLQIYNH+ESVALVVDN E+FNR+  TF
Sbjct: 151 ADNSCRWLVADQGMMASGAINVVRGSRSSVAELLQIYNHSESVALVVDNSEMFNRVANTF 210

Query: 151 RSKASTRCIILLWGENSSLAAEG-------------------------------LDAINL 210
            S  + R IILLWGE S L A+                                 +AI  
Sbjct: 211 YSTTTMRFIILLWGEKSELVAQQNKHIPVFTFTQLIDLGAESRTKLSKPEQRYMYEAIKS 270

Query: 211 DDIATLIYTSGTTGSPKAVMLTHRNFLHQIKNLWDAIPAKVGDKFLSMLPPWHAYERACE 270
           D IATLIYTSGTTG+PK VMLTH+N LHQIKNLWD +PAKVGD+FLSMLPPWHAYERACE
Sbjct: 271 DSIATLIYTSGTTGNPKGVMLTHQNLLHQIKNLWDIVPAKVGDRFLSMLPPWHAYERACE 330

Query: 271 YFIFTHGVEQTYTTTRKLKVFQTPLYLNCQDDLRDYQPDYLVSVPLV 287
           YFIFT G++Q YTT R LK           DDL  YQP YL+SVPLV
Sbjct: 331 YFIFTCGIQQQYTTVRNLK-----------DDLERYQPHYLISVPLV 366

[Close]
BLAST of CmaCh08G008740 vs. NCBI nr
Match: gi|674932246|emb|CDY01190.1| (BnaA04g06290D [Brassica napus])

HSP 1 Score: 349.7 bits (896), Expect = 4.7e-93
Identity = 181/307 (58.96%), Postives = 210/307 (68.40%), Query Frame = 1 

Query: 5   GAAISTISRHSLQSSPDFRISCQSKVLVPDIWRSSAERYGDRVVLLDPYHNPASKITYKQ 64
           G  ++ I   S  S  D  +       VPDIWRSSA++YGD+V L+DPYH+P  K+TY Q
Sbjct: 68  GKEVTPIIESSSLSGGDAALRSNEWKAVPDIWRSSADKYGDKVALVDPYHDPPLKLTYNQ 127

Query: 65  LEQSILNFCEGLRAIGIKPDEKIALFSDNSCRLACCRSSIMSMGAINVVRGSRSSSEELL 124
           LEQ IL+F EGLRA+G+KPDEKIALF+DNSCR       IMS GA+NVVRGSRSS EELL
Sbjct: 128 LEQEILDFAEGLRAVGVKPDEKIALFADNSCRWLVSDQGIMSTGAVNVVRGSRSSVEELL 187

Query: 125 QIYNHNESVALVVDNPELFNRIVETFRSKASTRCIILLWGENSSLAAEGLD--------- 184
           QIY H+ESVALVVDNPE FNRI +TF SKAS R +ILLWGE SSL  +G+          
Sbjct: 188 QIYQHSESVALVVDNPEFFNRIADTFTSKASLRFLILLWGEKSSLVTQGMQIPVYSYTDI 247

Query: 185 ----------------AINLDDIATLIYTSGTTGSPKAVMLTHRNFLHQIKNLWDAIPAK 244
                            IN DD A ++YTSGTTG+PK VMLTHRN LHQIK+L   +PA+
Sbjct: 248 KNLGQEKRAGSNDTRKPINPDDTAAIMYTSGTTGNPKGVMLTHRNLLHQIKHLSAYVPAE 307

Query: 245 VGDKFLSMLPPWHAYERACEYFIFTHGVEQTYTTTRKLKVFQTPLYLNCQDDLRDYQPDY 287
            GD+FLSMLP WHAYERACEYFIFT GVEQ YT+ R LK           DDL+ YQP Y
Sbjct: 308 AGDRFLSMLPSWHAYERACEYFIFTCGVEQMYTSIRFLK-----------DDLKRYQPHY 363

[Close]
BLAST of CmaCh08G008740 vs. NCBI nr
Match: gi|923606579|ref|XP_013742873.1| (PREDICTED: long-chain-fatty-acid--[acyl-carrier-protein])

HSP 1 Score: 349.4 bits (895), Expect = 6.1e-93
Identity = 181/307 (58.96%), Postives = 210/307 (68.40%), Query Frame = 1 

Query: 5   GAAISTISRHSLQSSPDFRISCQSKVLVPDIWRSSAERYGDRVVLLDPYHNPASKITYKQ 64
           G  ++ I   S  S  D  +       VPDIWRSSA++YGD+V L+DPYH+P  K+TY Q
Sbjct: 68  GKEVTPIIESSSLSGGDAALRSNEWKAVPDIWRSSADKYGDKVALVDPYHDPPLKLTYNQ 127

Query: 65  LEQSILNFCEGLRAIGIKPDEKIALFSDNSCRLACCRSSIMSMGAINVVRGSRSSSEELL 124
           LEQ IL+F EGLRA+G+KPDEKIALF+DNSCR       IMS GA+NVVRGSRSS EELL
Sbjct: 128 LEQEILDFSEGLRAVGVKPDEKIALFADNSCRWLVSDQGIMSTGAVNVVRGSRSSVEELL 187

Query: 125 QIYNHNESVALVVDNPELFNRIVETFRSKASTRCIILLWGENSSLAAEGLD--------- 184
           QIY H+ESVALVVDNPE FNRI +TF SKAS R +ILLWGE SSL  +G+          
Sbjct: 188 QIYQHSESVALVVDNPEFFNRIADTFTSKASLRFLILLWGEKSSLVTQGMQIPVYSYTDI 247

Query: 185 ----------------AINLDDIATLIYTSGTTGSPKAVMLTHRNFLHQIKNLWDAIPAK 244
                            IN DD A ++YTSGTTG+PK VMLTHRN LHQIK+L   +PA+
Sbjct: 248 KNLGQEKRAGSNDTRKPINPDDTAAIMYTSGTTGNPKGVMLTHRNLLHQIKHLSAYVPAE 307

Query: 245 VGDKFLSMLPPWHAYERACEYFIFTHGVEQTYTTTRKLKVFQTPLYLNCQDDLRDYQPDY 287
            GD+FLSMLP WHAYERACEYFIFT GVEQ YT+ R LK           DDL+ YQP Y
Sbjct: 308 AGDRFLSMLPSWHAYERACEYFIFTCGVEQMYTSIRFLK-----------DDLKRYQPHY 363

The following BLAST results are available for this feature:
BLAST of CmaCh08G008740 vs. Swiss-Prot
Match NameE-valueIdentityDescription
AAE15_ARATH7.2e-8659.23Long-chain-fatty-acid--[acyl-carrier-protein] ligase AEE15, chloroplastic OS=Ara... [more]
AAE16_ARATH1.2e-8356.79Probable acyl-activating enzyme 16, chloroplastic OS=Arabidopsis thaliana GN=AAE... [more]
FAC15_MYCMM4.9e-1829.10Long-chain-fatty-acid--CoA ligase FadD15 OS=Mycobacterium marinum (strain ATCC B... [more]
MENE_BACWK1.6e-1328.162-succinylbenzoate--CoA ligase OS=Bacillus weihenstephanensis (strain KBAB4) GN=... [more]
ACSL1_MOUSE4.8e-1325.95Long-chain-fatty-acid--CoA ligase 1 OS=Mus musculus GN=Acsl1 PE=1 SV=2[more]
BLAST of CmaCh08G008740 vs. TrEMBL
Match NameE-valueIdentityDescription
A0A022QUF6_ERYGU2.4e-9661.38Uncharacterized protein OS=Erythranthe guttata GN=MIMGU_mgv1a001985mg PE=4 SV=1[more]
V7C657_PHAVU1.1e-9361.67Uncharacterized protein OS=Phaseolus vulgaris GN=PHAVU_003G051300g PE=4 SV=1[more]
A0A078EGI0_BRANA3.3e-9358.96BnaA04g06290D protein OS=Brassica napus GN=BnaA04g06290D PE=4 SV=1[more]
M4EVC4_BRARP4.3e-9358.96Uncharacterized protein OS=Brassica rapa subsp. pekinensis PE=4 SV=1[more]
A0A0D3BYS9_BRAOL9.5e-9358.31Uncharacterized protein OS=Brassica oleracea var. oleracea GN=AAE15 PE=4 SV=1[more]
BLAST of CmaCh08G008740 vs. TAIR10
Match NameE-valueIdentityDescription
AT4G14070.14.1e-8759.23 acyl-activating enzyme 15[more]
AT3G23790.16.5e-8556.79 AMP-dependent synthetase and ligase family protein[more]
AT2G04350.13.0e-1322.87 AMP-dependent synthetase and ligase family protein[more]
AT1G77590.15.6e-1223.08 long chain acyl-CoA synthetase 9[more]
AT4G11030.12.1e-1127.31 AMP-dependent synthetase and ligase family protein[more]
BLAST of CmaCh08G008740 vs. NCBI nr
Match NameE-valueIdentityDescription
gi|645232163|ref|XP_008222738.1|1.1e-9763.76PREDICTED: probable acyl-activating enzyme 16, chloroplastic isoform X1 [Prunus ... [more]
gi|848888612|ref|XP_012844494.1|3.5e-9661.38PREDICTED: probable acyl-activating enzyme 16, chloroplastic [Erythranthe guttat... [more]
gi|593781169|ref|XP_007153625.1|1.6e-9361.67hypothetical protein PHAVU_003G051300g [Phaseolus vulgaris][more]
gi|674932246|emb|CDY01190.1|4.7e-9358.96BnaA04g06290D [Brassica napus][more]
gi|923606579|ref|XP_013742873.1|6.1e-9358.96PREDICTED: long-chain-fatty-acid--[acyl-carrier-protein][more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR000873AMP-dep_Synth/Lig
IPR020845AMP-binding_CS
Vocabulary: Molecular Function
TermDefinition
GO:0003824catalytic activity
Vocabulary: Biological Process
TermDefinition
GO:0008152metabolic process
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008152 metabolic process
cellular_component GO:0005575 cellular_component
molecular_function GO:0003824 catalytic activity
molecular_function GO:0016874 ligase activity
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh08G008740.1CmaCh08G008740.1mRNA


InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima
Date Performed: 2017-05-20
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000873AMP-dependent synthetase/ligasePFAMPF00501AMP-bindingcoord: 38..284
score: 1.1
IPR020845AMP-binding, conserved sitePROSITEPS00455AMP_BINDINGcoord: 185..196
scor
NoneNo IPR availableGENE3DG3DSA:3.40.50.980coord: 192..260
score: 2.2E-19coord: 32..191
score: 1.0
NoneNo IPR availablePANTHERPTHR24096FAMILY NOT NAMEDcoord: 32..286
score: 1.2E
NoneNo IPR availablePANTHERPTHR24096:SF199LONG-CHAIN-FATTY-ACID--[ACYL-CARRIER-PROTEIN] LIGASE AEE15, CHLOROPLASTIC-RELATEDcoord: 32..286
score: 1.2E
NoneNo IPR availableunknownSSF56801Acetyl-CoA synthetase-likecoord: 28..285
score: 3.92