CmoCh07G007770 (gene) Cucurbita moschata (Rifu)

Overview
NameCmoCh07G007770
Typegene
OrganismCucurbita moschata (Cucurbita moschata (Rifu))
DescriptionGeranylgeranyl-diphosphate synthase
LocationCmo_Chr07 : 3511881 .. 3512999 (+)
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAGCTCTGTCAGTTTCGCGAATCCATTGATTCAGAGCAACTCTGTTCTCAACAATTCTCAGAAATTCAATCAAAAATCCTTTTCTTTTCTTCATCCATGGAACCCCTTGTCGTCTTTTTCCTCCAAATCCATTAAAAAACCTTCCTTTAACCTCCGCATATCGGCGGCGCAAACCAGAGTAGAAACAGAGCTCCGCCCGGTTGTTGAACAAAATGACCAAAAAGGGGTCTTCAGTTTCAAAGACTACATGGTGCAGAAGGCTAATTTGGTAAATCAAGCTTTGAACGACGCCGTTTCCCTGCGGGACCCACTGAAGATTCACGAGGCCATGCGTTACTCTCTCCTCGCCGGCGGGAAGCGTGTACGGCCAGTGCTCTGTCTCGCTGCCTGCGAGCTTGTTGGTGGTTCTGAGTCTGCCGCTATGCCGGCCGCTTGCGCCACTGAAATGATCCACACCATGTCTTTAATCCACGATGATCTTCCATGCATGGACAACGACGACCTCCGGCGAGGAAAGCCGACGAGCCACCGTATGTTTGGTGAGGACATAGCCATCCTCGCCGGTGACGCTCTTTTAGCCTTCGCATTTGAGCACATCGCAGTGGCGACAGAGGCGGGGGTATCGCCGGAGAGAGTGGTCCAAGCAATTGGAGAATTGGCAAAAGCCACCGGAACATCAGGGTTAGTCGCCGGGCAAGTGGTGGACATAGACTCCGAAGGCTCTAACGACGTCGGATTGGAACTCCTCGAGTACATCCACGTACACAAAACGGCAGCGCTTCTGGAAGCCGCCGCCGTCATGGGAGCAATCATAGGCGGCGGAAGCAACACCGAAGTGGAGAAGGTGAGGAAATTCGCCCGACAGATCGGATTACTCTTCCAAGTGGTAGACGACATTCTAGATGTGACAAAGTCGTCGGAGGAATTGGGGAAGACGGCCGGAAAAGACCTGGCGGCGGACAAAGCGACGTATCCAAAATTACTCGGAATCGAAAAATCAAAGGAATTTGCGGAGACGCTAAAGAGAGAAGCCGGAGAAGCGCTAGCCGGATTTGATCCGGCGAAGGCGGAGCCGTTAATTGCCCTGTCGAATTACATAGCCTATAGGCAAAATTGA

mRNA sequence

ATGAGCTCTGTCAGTTTCGCGAATCCATTGATTCAGAGCAACTCTGTTCTCAACAATTCTCAGAAATTCAATCAAAAATCCTTTTCTTTTCTTCATCCATGGAACCCCTTGTCGTCTTTTTCCTCCAAATCCATTAAAAAACCTTCCTTTAACCTCCGCATATCGGCGGCGCAAACCAGAGTAGAAACAGAGCTCCGCCCGGTTGTTGAACAAAATGACCAAAAAGGGGTCTTCAGTTTCAAAGACTACATGGTGCAGAAGGCTAATTTGGTAAATCAAGCTTTGAACGACGCCGTTTCCCTGCGGGACCCACTGAAGATTCACGAGGCCATGCGTTACTCTCTCCTCGCCGGCGGGAAGCGTGTACGGCCAGTGCTCTGTCTCGCTGCCTGCGAGCTTGTTGGTGGTTCTGAGTCTGCCGCTATGCCGGCCGCTTGCGCCACTGAAATGATCCACACCATGTCTTTAATCCACGATGATCTTCCATGCATGGACAACGACGACCTCCGGCGAGGAAAGCCGACGAGCCACCGTATGTTTGGTGAGGACATAGCCATCCTCGCCGGTGACGCTCTTTTAGCCTTCGCATTTGAGCACATCGCAGTGGCGACAGAGGCGGGGGTATCGCCGGAGAGAGTGGTCCAAGCAATTGGAGAATTGGCAAAAGCCACCGGAACATCAGGGTTAGTCGCCGGGCAAGTGGTGGACATAGACTCCGAAGGCTCTAACGACGTCGGATTGGAACTCCTCGAGTACATCCACGTACACAAAACGGCAGCGCTTCTGGAAGCCGCCGCCGTCATGGGAGCAATCATAGGCGGCGGAAGCAACACCGAAGTGGAGAAGGTGAGGAAATTCGCCCGACAGATCGGATTACTCTTCCAAGTGGTAGACGACATTCTAGATGTGACAAAGTCGTCGGAGGAATTGGGGAAGACGGCCGGAAAAGACCTGGCGGCGGACAAAGCGACGTATCCAAAATTACTCGGAATCGAAAAATCAAAGGAATTTGCGGAGACGCTAAAGAGAGAAGCCGGAGAAGCGCTAGCCGGATTTGATCCGGCGAAGGCGGAGCCGTTAATTGCCCTGTCGAATTACATAGCCTATAGGCAAAATTGA

Coding sequence (CDS)

ATGAGCTCTGTCAGTTTCGCGAATCCATTGATTCAGAGCAACTCTGTTCTCAACAATTCTCAGAAATTCAATCAAAAATCCTTTTCTTTTCTTCATCCATGGAACCCCTTGTCGTCTTTTTCCTCCAAATCCATTAAAAAACCTTCCTTTAACCTCCGCATATCGGCGGCGCAAACCAGAGTAGAAACAGAGCTCCGCCCGGTTGTTGAACAAAATGACCAAAAAGGGGTCTTCAGTTTCAAAGACTACATGGTGCAGAAGGCTAATTTGGTAAATCAAGCTTTGAACGACGCCGTTTCCCTGCGGGACCCACTGAAGATTCACGAGGCCATGCGTTACTCTCTCCTCGCCGGCGGGAAGCGTGTACGGCCAGTGCTCTGTCTCGCTGCCTGCGAGCTTGTTGGTGGTTCTGAGTCTGCCGCTATGCCGGCCGCTTGCGCCACTGAAATGATCCACACCATGTCTTTAATCCACGATGATCTTCCATGCATGGACAACGACGACCTCCGGCGAGGAAAGCCGACGAGCCACCGTATGTTTGGTGAGGACATAGCCATCCTCGCCGGTGACGCTCTTTTAGCCTTCGCATTTGAGCACATCGCAGTGGCGACAGAGGCGGGGGTATCGCCGGAGAGAGTGGTCCAAGCAATTGGAGAATTGGCAAAAGCCACCGGAACATCAGGGTTAGTCGCCGGGCAAGTGGTGGACATAGACTCCGAAGGCTCTAACGACGTCGGATTGGAACTCCTCGAGTACATCCACGTACACAAAACGGCAGCGCTTCTGGAAGCCGCCGCCGTCATGGGAGCAATCATAGGCGGCGGAAGCAACACCGAAGTGGAGAAGGTGAGGAAATTCGCCCGACAGATCGGATTACTCTTCCAAGTGGTAGACGACATTCTAGATGTGACAAAGTCGTCGGAGGAATTGGGGAAGACGGCCGGAAAAGACCTGGCGGCGGACAAAGCGACGTATCCAAAATTACTCGGAATCGAAAAATCAAAGGAATTTGCGGAGACGCTAAAGAGAGAAGCCGGAGAAGCGCTAGCCGGATTTGATCCGGCGAAGGCGGAGCCGTTAATTGCCCTGTCGAATTACATAGCCTATAGGCAAAATTGA
Homology
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BLAST of CmoCh07G007770 vs. Swiss-Prot
Match: GGPPS_HEVBR (Geranylgeranyl pyrophosphate synthase, chloroplastic OS=Hevea brasiliensis GN=GGPS PE=1 SV=1)

HSP 1 Score: 471.5 bits (1212), Expect = 8.7e-132
Identity = 254/376 (67.55%), Postives = 303/376 (80.59%), Query Frame = 1 

Query: 1   MSSVSFANPLIQSNSVLNNSQKFNQKSFSFLHPWNPLSS----FSSKSIKKPSFNLRISA 60
           MSSV+  +  + ++ VLN + +   KS SF  P+NPL S    F+ +  ++P  +  +SA
Sbjct: 1   MSSVNLGS-WVHTSYVLNQATRSRSKSKSFSLPFNPLKSLAISFAYRKSERPISS--VSA 60

Query: 61  AQTRVETELRPVVEQNDQKGVFSFKDYMVQKANLVNQALNDAVSLRDPLKIHEAMRYSLL 120
             T+ E  L+   EQN+    F FK YM+QK N +NQAL  A+ L++P KIHE+MRYSLL
Sbjct: 61  IITKEEETLQE--EQNNPPPSFDFKSYMLQKGNSINQALEAAIPLQEPAKIHESMRYSLL 120

Query: 121 AGGKRVRPVLCLAACELVGGSESAAMPAACATEMIHTMSLIHDDLPCMDNDDLRRGKPTS 180
           AGGKRVRP LCLAACELVGG++S AMPAACA EMIHTMSLIHDDLPCMDNDDLRRGKPT+
Sbjct: 121 AGGKRVRPALCLAACELVGGNDSMAMPAACAVEMIHTMSLIHDDLPCMDNDDLRRGKPTN 180

Query: 181 HRMFGEDIAILAGDALLAFAFEHIAVATEAGVSPERVVQAIGELAKATGTSGLVAGQVVD 240
           H +FGED+A+LAGDALLAFAFEHIAV+T   VS  R+V+A+GELAKA G  GLVAGQVVD
Sbjct: 181 HIVFGEDVAVLAGDALLAFAFEHIAVST-LNVSSARIVRAVGELAKAIGAEGLVAGQVVD 240

Query: 241 IDSEGSNDVGLELLEYIHVHKTAALLEAAAVMGAIIGGGSNTEVEKVRKFARQIGLLFQV 300
           I+SEGS++V LE LE+IH+HKTA LLE A V+GAI+GGG++ EVEK+RK+AR IGLLFQV
Sbjct: 241 INSEGSSEVDLEKLEFIHIHKTAKLLEGAVVLGAILGGGTDEEVEKLRKYARDIGLLFQV 300

Query: 301 VDDILDVTKSSEELGKTAGKDLAADKATYPKLLGIEKSKEFAETLKREAGEALAGFDPAK 360
           VDDILDVTKSS+ELGKTAGKDL ADK TYPKLLGIEKS+EFAE L +EA E LAGFDP K
Sbjct: 301 VDDILDVTKSSQELGKTAGKDLVADKVTYPKLLGIEKSREFAEKLNKEAQEQLAGFDPEK 360

Query: 361 AEPLIALSNYIAYRQN 373
           A PLIAL+NYIA+RQN
Sbjct: 361 AAPLIALANYIAHRQN 370

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BLAST of CmoCh07G007770 vs. Swiss-Prot
Match: GGPPS_CATRO (Geranylgeranyl pyrophosphate synthase, chloroplastic OS=Catharanthus roseus GN=GGPS1 PE=3 SV=1)

HSP 1 Score: 457.2 bits (1175), Expect = 1.7e-127
Identity = 241/347 (69.45%), Postives = 284/347 (81.84%), Query Frame = 1 

Query: 36  PLSSFSSKSIKKPSFN---LRISAAQTRVETELRPVVEQNDQKGV-------FSFKDYMV 95
           P+S F S +I+KP F+   L ISA  T+ +T+     E   +K V       F FK YM+
Sbjct: 14  PISFFLSGTIRKPIFSCSRLSISAIITKEQTQEES--ESKSKKEVAFSSSSSFDFKAYMI 73

Query: 96  QKANLVNQALNDAVSLRDPLKIHEAMRYSLLAGGKRVRPVLCLAACELVGGSESAAMPAA 155
            KAN VN+AL DAV +R+PLKIHE+MRYSLLAGGKRVRP+LC+AACEL GG+ES AMP+A
Sbjct: 74  GKANSVNKALEDAVLVREPLKIHESMRYSLLAGGKRVRPMLCIAACELFGGTESVAMPSA 133

Query: 156 CATEMIHTMSLIHDDLPCMDNDDLRRGKPTSHRMFGEDIAILAGDALLAFAFEHIAVATE 215
           CA EMIHTMSL+HDDLPCMDNDDLRRGKPT+H++FGED+A+LAGDALLAFAFEHIA AT+
Sbjct: 134 CAVEMIHTMSLMHDDLPCMDNDDLRRGKPTNHKVFGEDVAVLAGDALLAFAFEHIATATK 193

Query: 216 AGVSPERVVQAIGELAKATGTSGLVAGQVVDIDSEGSNDVGLELLEYIHVHKTAALLEAA 275
            GVS ER+V+ +GELAK  G+ GLVAGQVVD+ SEG  DVGLE LE+IH+HKTAALLE +
Sbjct: 194 -GVSSERIVRVVGELAKCIGSEGLVAGQVVDVCSEGIADVGLEHLEFIHIHKTAALLEGS 253

Query: 276 AVMGAIIGGGSNTEVEKVRKFARQIGLLFQVVDDILDVTKSSEELGKTAGKDLAADKATY 335
            V+GAI+GG ++ ++ K+RKFAR IGLLFQVVDDILDVTKSS+ELGKTAGKDL ADK TY
Sbjct: 254 VVLGAIVGGANDEQISKLRKFARCIGLLFQVVDDILDVTKSSQELGKTAGKDLVADKVTY 313

Query: 336 PKLLGIEKSKEFAETLKREAGEALAGFDPAKAEPLIALSNYIAYRQN 373
           PKLLGI+KS+EFAE L REA E LA FDP KA PLIAL+NYIAYR N
Sbjct: 314 PKLLGIDKSREFAEKLNREAQEQLAEFDPEKAAPLIALANYIAYRDN 357

[Close]
BLAST of CmoCh07G007770 vs. Swiss-Prot
Match: GGPP1_ARATH (Heterodimeric geranylgeranyl pyrophosphate synthase large subunit 1, chloroplastic OS=Arabidopsis thaliana GN=GGPPS1 PE=1 SV=2)

HSP 1 Score: 452.6 bits (1163), Expect = 4.2e-126
Identity = 240/337 (71.22%), Postives = 278/337 (82.49%), Query Frame = 1 

Query: 39  SFSSKSIKKPSFNLRISAAQTRVETELRPVVEQNDQKGVFSFKDYMVQKANLVNQALNDA 98
           SF SK     S ++  S+  T+ E  LR       +   F F  Y++ KA LVN+AL+ A
Sbjct: 41  SFRSKRTVSSSSSIVSSSVVTK-EDNLR-----QSEPSSFDFMSYIITKAELVNKALDSA 100

Query: 99  VSLRDPLKIHEAMRYSLLAGGKRVRPVLCLAACELVGGSESAAMPAACATEMIHTMSLIH 158
           V LR+PLKIHEAMRYSLLAGGKRVRPVLC+AACELVGG ES AMPAACA EMIHTMSLIH
Sbjct: 101 VPLREPLKIHEAMRYSLLAGGKRVRPVLCIAACELVGGEESTAMPAACAVEMIHTMSLIH 160

Query: 159 DDLPCMDNDDLRRGKPTSHRMFGEDIAILAGDALLAFAFEHIAVATEAG-VSPERVVQAI 218
           DDLPCMDNDDLRRGKPT+H++FGED+A+LAGDALL+FAFEH+A AT +  VSP RVV+A+
Sbjct: 161 DDLPCMDNDDLRRGKPTNHKVFGEDVAVLAGDALLSFAFEHLASATSSDVVSPVRVVRAV 220

Query: 219 GELAKATGTSGLVAGQVVDIDSEG--SNDVGLELLEYIHVHKTAALLEAAAVMGAIIGGG 278
           GELAKA GT GLVAGQVVDI SEG   NDVGLE LE+IH+HKTAALLEA+AV+GAI+GGG
Sbjct: 221 GELAKAIGTEGLVAGQVVDISSEGLDLNDVGLEHLEFIHLHKTAALLEASAVLGAIVGGG 280

Query: 279 SNTEVEKVRKFARQIGLLFQVVDDILDVTKSSEELGKTAGKDLAADKATYPKLLGIEKSK 338
           S+ E+E++RKFAR IGLLFQVVDDILDVTKSS+ELGKTAGKDL ADK TYPK++G+EKS+
Sbjct: 281 SDDEIERLRKFARCIGLLFQVVDDILDVTKSSKELGKTAGKDLIADKLTYPKIMGLEKSR 340

Query: 339 EFAETLKREAGEALAGFDPAKAEPLIALSNYIAYRQN 373
           EFAE L REA + L GFD  K  PL+AL+NYIAYRQN
Sbjct: 341 EFAEKLNREARDQLLGFDSDKVAPLLALANYIAYRQN 371

[Close]
BLAST of CmoCh07G007770 vs. Swiss-Prot
Match: GGPPS_SINAL (Geranylgeranyl pyrophosphate synthase, chloroplastic/chromoplastic OS=Sinapis alba GN=GGPS1 PE=1 SV=1)

HSP 1 Score: 441.8 bits (1135), Expect = 7.4e-123
Identity = 233/337 (69.14%), Postives = 272/337 (80.71%), Query Frame = 1 

Query: 38  SSFSSKSIKKPSFNLRISAAQTRVETELRPVVEQNDQKGVFSFKDYMVQKANLVNQALND 97
           SS SS  I K   NL+ S++                    F F  Y+++KA+ VN+AL+ 
Sbjct: 49  SSSSSSLITKEDNNLKSSSSS-------------------FDFMSYIIRKADSVNKALDS 108

Query: 98  AVSLRDPLKIHEAMRYSLLAGGKRVRPVLCLAACELVGGSESAAMPAACATEMIHTMSLI 157
           AV LR+PLKIHEAMRYSLLAGGKRVRPVLC+AACELVGG ES AMPA CA EMIHTMSLI
Sbjct: 109 AVPLREPLKIHEAMRYSLLAGGKRVRPVLCIAACELVGGEESLAMPARCAVEMIHTMSLI 168

Query: 158 HDDLPCMDNDDLRRGKPTSHRMFGEDIAILAGDALLAFAFEHIAVATEAGVSPERVVQAI 217
           HDDLPCMDNDDLRRGKPT+H+++GED+A+LAGDALL+FAFEH+A AT + VSP RVV+A+
Sbjct: 169 HDDLPCMDNDDLRRGKPTNHKVYGEDVAVLAGDALLSFAFEHLASATSSEVSPARVVRAV 228

Query: 218 GELAKATGTSGLVAGQVVDIDSEG--SNDVGLELLEYIHVHKTAALLEAAAVMGAIIGGG 277
           GELAKA GT GLVAGQVVDI SEG   N+VGLE L++IH+HKTAALLEA+AV+G IIGGG
Sbjct: 229 GELAKAIGTEGLVAGQVVDISSEGLDLNNVGLEHLKFIHLHKTAALLEASAVLGGIIGGG 288

Query: 278 SNTEVEKVRKFARQIGLLFQVVDDILDVTKSSEELGKTAGKDLAADKATYPKLLGIEKSK 337
           S+ E+E++RKFAR IGLLFQVVDDILDVTKSS+ELGKTAGKDL ADK TYPKL+G+EKS+
Sbjct: 289 SDEEIERLRKFARCIGLLFQVVDDILDVTKSSQELGKTAGKDLIADKLTYPKLMGLEKSR 348

Query: 338 EFAETLKREAGEALAGFDPAKAEPLIALSNYIAYRQN 373
           EFAE L  EA + L GFD  K  PL+AL+NYIA RQN
Sbjct: 349 EFAEKLNTEARDQLLGFDSDKVAPLLALANYIANRQN 366

[Close]
BLAST of CmoCh07G007770 vs. Swiss-Prot
Match: GGPP7_ARATH (Geranylgeranyl pyrophosphate synthase 7, chloroplastic OS=Arabidopsis thaliana GN=At2g18620 PE=2 SV=1)

HSP 1 Score: 439.9 bits (1130), Expect = 2.8e-122
Identity = 223/297 (75.08%), Postives = 260/297 (87.54%), Query Frame = 1 

Query: 78  FSFKDYMVQKANLVNQALNDAVSLRDPLKIHEAMRYSLLAGGKRVRPVLCLAACELVGGS 137
           F F  YM+ KAN VN+AL+ AVSLR+P+KIHEA+RYSLLA GKRVRPVLC+AACELVGG 
Sbjct: 53  FDFTSYMIGKANAVNEALDSAVSLREPIKIHEAIRYSLLARGKRVRPVLCIAACELVGGE 112

Query: 138 ESAAMPAACATEMIHTMSLIHDDLPCMDNDDLRRGKPTSHRMFGEDIAILAGDALLAFAF 197
           ES A+PAACA EMIHTMSLIHDDLPCMDNDDLRRGKPT+H++FGED+A+LAGDAL++FAF
Sbjct: 113 ESVALPAACAVEMIHTMSLIHDDLPCMDNDDLRRGKPTNHKVFGEDVAVLAGDALISFAF 172

Query: 198 EHIAVATEAGVSPERVVQAIGELAKATGTSGLVAGQVVDIDSEG--SNDVGLELLEYIHV 257
           EH+A +T   VSP RVV+AIGELAKA G+ GLVAGQVVD+ S G   NDVGLE+LE+IHV
Sbjct: 173 EHLATST--AVSPARVVRAIGELAKAIGSKGLVAGQVVDLTSGGMDQNDVGLEVLEFIHV 232

Query: 258 HKTAALLEAAAVMGAIIGGGSNTEVEKVRKFARQIGLLFQVVDDILDVTKSSEELGKTAG 317
           HKTA LLEAA V+GAI+GGGS+ EVEK+R+FAR IGLLFQVVDDILDVTKSSEELGKTAG
Sbjct: 233 HKTAVLLEAATVLGAIVGGGSDEEVEKLRRFARCIGLLFQVVDDILDVTKSSEELGKTAG 292

Query: 318 KDLAADKATYPKLLGIEKSKEFAETLKREAGEALAGFDPAKAEPLIALSNYIAYRQN 373
           KDL ADK TYPKL+G+EKSK+FA+ L  +A E L GFD ++ +PL+AL+NYIA RQN
Sbjct: 293 KDLIADKLTYPKLMGLEKSKDFADKLLSDAHEQLHGFDSSRVKPLLALANYIAKRQN 347

[Close]
BLAST of CmoCh07G007770 vs. TrEMBL
Match: A0A0A0KFZ6_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_6G487640 PE=3 SV=1)

HSP 1 Score: 554.7 bits (1428), Expect = 8.7e-155
Identity = 296/373 (79.36%), Postives = 330/373 (88.47%), Query Frame = 1 

Query: 2   SSVSFANPLIQSNSVLNNSQKFNQKSFSFLHPW-NPLSSF-SSKSIKKPSFNLRISAAQT 61
           SS++F NPLIQS+SV  NS KF    F  LHP  +P+S   SSKS K  S    ISAA T
Sbjct: 3   SSLNFTNPLIQSSSVFTNSHKFKSNPFFLLHPLTSPISLLISSKSTKNSSSRRPISAALT 62

Query: 62  RVETELRPVVEQND-QKGVFSFKDYMVQKANLVNQALNDAVSLRDPLKIHEAMRYSLLAG 121
             +TEL P +EQND Q   F F+DYM+QK+NLVNQAL++AVSL+DPLKIHEAMRYSLLAG
Sbjct: 63  TEQTELHPQLEQNDVQNPPFRFRDYMIQKSNLVNQALDEAVSLQDPLKIHEAMRYSLLAG 122

Query: 122 GKRVRPVLCLAACELVGGSESAAMPAACATEMIHTMSLIHDDLPCMDNDDLRRGKPTSHR 181
           GKRVRPVLCLAAC+LVGGSESAA+PAAC+ EMIHTMSLIHDDLPCMDNDDLRRGKPT+HR
Sbjct: 123 GKRVRPVLCLAACDLVGGSESAAIPAACSIEMIHTMSLIHDDLPCMDNDDLRRGKPTNHR 182

Query: 182 MFGEDIAILAGDALLAFAFEHIAVATEAGVSPERVVQAIGELAKATGTSGLVAGQVVDID 241
           MFGED+A+LAGDALL+FAFEH+A AT+A VSPERVV+AIGELAKATGT GLVAGQVVDID
Sbjct: 183 MFGEDVAVLAGDALLSFAFEHMA-ATKAEVSPERVVRAIGELAKATGTGGLVAGQVVDID 242

Query: 242 SEGSNDVGLELLEYIHVHKTAALLEAAAVMGAIIGGGSNTEVEKVRKFARQIGLLFQVVD 301
           SEG++DVGLELLEYIHVHKTAALLEAAAVMGAI+GGGS  E+EK+R FAR+IGLLFQVVD
Sbjct: 243 SEGADDVGLELLEYIHVHKTAALLEAAAVMGAIVGGGSEEEIEKLRIFARRIGLLFQVVD 302

Query: 302 DILDVTKSSEELGKTAGKDLAADKATYPKLLGIEKSKEFAETLKREAGEALAGFDPAKAE 361
           DILDVTKS+EELGKTAGKD+AA+KATYPKL+GIEKSKEFAE L+ EA EALAGFDP KAE
Sbjct: 303 DILDVTKSTEELGKTAGKDVAAEKATYPKLMGIEKSKEFAEKLREEAIEALAGFDPVKAE 362

Query: 362 PLIALSNYIAYRQ 372
           PLIALSNYIAYRQ
Sbjct: 363 PLIALSNYIAYRQ 374

[Close]
BLAST of CmoCh07G007770 vs. TrEMBL
Match: A0A061DHH0_THECC (Geranylgeranyl pyrophosphate synthase 1 OS=Theobroma cacao GN=TCM_000455 PE=3 SV=1)

HSP 1 Score: 487.3 bits (1253), Expect = 1.7e-134
Identity = 257/362 (70.99%), Postives = 294/362 (81.22%), Query Frame = 1 

Query: 11  IQSNSVLNNSQKFNQKSFSFLHPWNPLSSFSSKSIKKPSFNLRISAAQTRVETELRPVVE 70
           +QS S+ N + +          P+NPL + SS + KK S     S +    + +     E
Sbjct: 10  VQSYSIFNQASRSKSIPLPRSFPFNPLKNLSSSTPKKRSLTSLPSISAVLTKEDTIKEEE 69

Query: 71  QNDQKGVFSFKDYMVQKANLVNQALNDAVSLRDPLKIHEAMRYSLLAGGKRVRPVLCLAA 130
           Q+ QK  F FK YMVQKA+ VNQAL+ AVSLRDP+KIHEAMRYSLLAGGKRVRP LCLAA
Sbjct: 70  QDPQKPSFDFKSYMVQKASTVNQALDSAVSLRDPVKIHEAMRYSLLAGGKRVRPALCLAA 129

Query: 131 CELVGGSESAAMPAACATEMIHTMSLIHDDLPCMDNDDLRRGKPTSHRMFGEDIAILAGD 190
           CELVGG ES AMPAACA EMIHTMSLIHDDLPCMDNDDLRRGKPT+H++FGEDIA+LAGD
Sbjct: 130 CELVGGQESMAMPAACAVEMIHTMSLIHDDLPCMDNDDLRRGKPTNHKVFGEDIAVLAGD 189

Query: 191 ALLAFAFEHIAVATEAGVSPERVVQAIGELAKATGTSGLVAGQVVDIDSEGSNDVGLELL 250
           ALLAFAFEHIAV+T  GV+P RVV+A+GELAKA GT GLVAGQVVDI+SEG  DVGL+ L
Sbjct: 190 ALLAFAFEHIAVST-VGVTPGRVVKAVGELAKAIGTEGLVAGQVVDINSEGLIDVGLDHL 249

Query: 251 EYIHVHKTAALLEAAAVMGAIIGGGSNTEVEKVRKFARQIGLLFQVVDDILDVTKSSEEL 310
           E+IHVHKTA LLEAA V+GAI+GGG + +VEK+RKFAR IGLLFQVVDDILDVTKSS++L
Sbjct: 250 EFIHVHKTAVLLEAAVVLGAILGGGCDEDVEKLRKFARYIGLLFQVVDDILDVTKSSKDL 309

Query: 311 GKTAGKDLAADKATYPKLLGIEKSKEFAETLKREAGEALAGFDPAKAEPLIALSNYIAYR 370
           GKTAGKDL ADK TYPKL+GIEKS+EFAE LK +A E L GFDP KA PL+AL+NYIAYR
Sbjct: 310 GKTAGKDLVADKVTYPKLMGIEKSREFAEKLKSDALELLQGFDPEKAAPLVALANYIAYR 369

Query: 371 QN 373
           QN
Sbjct: 370 QN 370

[Close]
BLAST of CmoCh07G007770 vs. TrEMBL
Match: A0A0D2SNN1_GOSRA (Uncharacterized protein OS=Gossypium raimondii GN=B456_007G206700 PE=3 SV=1)

HSP 1 Score: 486.1 bits (1250), Expect = 3.8e-134
Identity = 262/364 (71.98%), Postives = 297/364 (81.59%), Query Frame = 1 

Query: 11  IQSNSVLNNSQKFNQKSFSFLH--PWNPLSSFSSKSIKKPSFNLRISAAQTRVETELRPV 70
           +QS S+ N     + KSF      P+NPL++ SS ++KK +F+   S      + ++   
Sbjct: 10  VQSYSMPNQVSSIS-KSFPLPRSLPFNPLTTQSSSTLKKVNFSSLPSITSVLTKEDIVKE 69

Query: 71  VEQNDQKGVFSFKDYMVQKANLVNQALNDAVSLRDPLKIHEAMRYSLLAGGKRVRPVLCL 130
            EQ+ QK  F F  YM+QKA+ VNQAL  AVSLRDP+KIHEAMRYSLLAGGKRVRPVLCL
Sbjct: 70  EEQDPQKSSFDFNSYMLQKASAVNQALESAVSLRDPVKIHEAMRYSLLAGGKRVRPVLCL 129

Query: 131 AACELVGGSESAAMPAACATEMIHTMSLIHDDLPCMDNDDLRRGKPTSHRMFGEDIAILA 190
           AAC LVGG ES AMPAACA EMIHTMSLIHDDLPCMDNDDLRRGKPT+H++FGEDIA+LA
Sbjct: 130 AACALVGGRESMAMPAACAVEMIHTMSLIHDDLPCMDNDDLRRGKPTNHKVFGEDIAVLA 189

Query: 191 GDALLAFAFEHIAVATEAGVSPERVVQAIGELAKATGTSGLVAGQVVDIDSEGSNDVGLE 250
           GDALLAFAFEHIAV+T  GV P R+V+AIGELAKA G  GLVAGQVVDI SEG  DVGL+
Sbjct: 190 GDALLAFAFEHIAVST-VGVPPSRIVRAIGELAKAIGGEGLVAGQVVDIISEGLTDVGLD 249

Query: 251 LLEYIHVHKTAALLEAAAVMGAIIGGGSNTEVEKVRKFARQIGLLFQVVDDILDVTKSSE 310
            LE+IHVHKTAALLEAA V+GAI+GGG + EVEK+RKFAR IGLLFQVVDDILDVTKSS+
Sbjct: 250 HLEFIHVHKTAALLEAAVVLGAIVGGGCDEEVEKLRKFARCIGLLFQVVDDILDVTKSSK 309

Query: 311 ELGKTAGKDLAADKATYPKLLGIEKSKEFAETLKREAGEALAGFDPAKAEPLIALSNYIA 370
           ELGKTAGKDL ADK TYPKL+GI+KSKEFAE LK +A E L GFDPAKA PLIAL+NYIA
Sbjct: 310 ELGKTAGKDLVADKVTYPKLMGIQKSKEFAEKLKTDAIELLQGFDPAKAAPLIALANYIA 369

Query: 371 YRQN 373
           YRQN
Sbjct: 370 YRQN 371

[Close]
BLAST of CmoCh07G007770 vs. TrEMBL
Match: A0A0G2QS78_MALDO (Geranylgeranyl diphosphate synthase OS=Malus domestica GN=GGPPS-1 PE=2 SV=1)

HSP 1 Score: 483.4 bits (1243), Expect = 2.5e-133
Identity = 255/364 (70.05%), Postives = 303/364 (83.24%), Query Frame = 1 

Query: 12  QSNSVLNNSQKF-NQKSFSFLHPWNPLSSFSSKSI--KKPSFNLRISAAQTRVETELRPV 71
           Q+ S+LN + +  + +SF  LHP       SS +   ++P+    +SA  T+ ET L   
Sbjct: 13  QTCSMLNQAGRSRSSQSFHLLHPLKTTIPTSSAAPHRRRPTSPFAVSAVLTKQET-LTEE 72

Query: 72  VEQNDQKGVFSFKDYMVQKANLVNQALNDAVSLRDPLKIHEAMRYSLLAGGKRVRPVLCL 131
            ++  +K  F+FK YM+Q+A  VNQAL+ AVSLRDP+ IHEAMRYSLLAGGKRVRPVLCL
Sbjct: 73  FDEESRKPTFNFKSYMLQRAESVNQALDAAVSLRDPITIHEAMRYSLLAGGKRVRPVLCL 132

Query: 132 AACELVGGSESAAMPAACATEMIHTMSLIHDDLPCMDNDDLRRGKPTSHRMFGEDIAILA 191
           AACELVGGSES AMPAACA EMIHTMSLIHDDLPCMDNDDLRRGKPT+H++FGED+A+LA
Sbjct: 133 AACELVGGSESMAMPAACAVEMIHTMSLIHDDLPCMDNDDLRRGKPTNHKVFGEDVAVLA 192

Query: 192 GDALLAFAFEHIAVATEAGVSPERVVQAIGELAKATGTSGLVAGQVVDIDSEGSNDVGLE 251
           GDALLAFAFEHI+++T  GVSP R+V+A+GELAK+ G+ GLVAGQVVDIDSEG +DVGLE
Sbjct: 193 GDALLAFAFEHISLST-VGVSPARIVRAVGELAKSIGSEGLVAGQVVDIDSEGLSDVGLE 252

Query: 252 LLEYIHVHKTAALLEAAAVMGAIIGGGSNTEVEKVRKFARQIGLLFQVVDDILDVTKSSE 311
            LEYIH+HKTAALLE + V+G+I+GGGS++E+EK+R FAR IGLLFQVVDDILDVTKSS+
Sbjct: 253 QLEYIHLHKTAALLECSVVLGSILGGGSDSEIEKLRTFARYIGLLFQVVDDILDVTKSSQ 312

Query: 312 ELGKTAGKDLAADKATYPKLLGIEKSKEFAETLKREAGEALAGFDPAKAEPLIALSNYIA 371
           ELGKTAGKDL ADK TYPKLLGIEKS+EFAE L ++A E LAGFDP KA PLIAL+NYIA
Sbjct: 313 ELGKTAGKDLVADKVTYPKLLGIEKSREFAEKLNKDAKEQLAGFDPEKAAPLIALANYIA 372

Query: 372 YRQN 373
           YRQN
Sbjct: 373 YRQN 374

[Close]
BLAST of CmoCh07G007770 vs. TrEMBL
Match: D6Q1T4_MEDSA (Geranylgeranyl-diphosphate synthase OS=Medicago sativa GN=GGPPS PE=2 SV=1)

HSP 1 Score: 478.8 bits (1231), Expect = 6.1e-132
Identity = 249/344 (72.38%), Postives = 290/344 (84.30%), Query Frame = 1 

Query: 29  SFLHPWNPLSSFSSKSIKKPSFNLRISAAQTRVETELRPVVEQNDQKGVFSFKDYMVQKA 88
           SF     P+S    K   KP+ +  + +  T+ E      +E  +Q  +F+FK YM++KA
Sbjct: 33  SFYFTKIPISVSPIKPSSKPNSSFSVLSLLTKQEP-----IEAEEQNPIFNFKSYMIEKA 92

Query: 89  NLVNQALNDAVSLRDPLKIHEAMRYSLLAGGKRVRPVLCLAACELVGGSESAAMPAACAT 148
             VN+AL+DAVSLR+PLK+HEAMRYSLLAGGKRVRPVLCLAACELVGG+E  AMPAACA 
Sbjct: 93  TRVNKALDDAVSLREPLKVHEAMRYSLLAGGKRVRPVLCLAACELVGGTEPMAMPAACAV 152

Query: 149 EMIHTMSLIHDDLPCMDNDDLRRGKPTSHRMFGEDIAILAGDALLAFAFEHIAVATEAGV 208
           EMIHTMSLIHDDLPCMDNDDLRRGKPT+H++FGED+A+LAGDALLAFAFEHIAV+T AGV
Sbjct: 153 EMIHTMSLIHDDLPCMDNDDLRRGKPTNHKVFGEDVAVLAGDALLAFAFEHIAVST-AGV 212

Query: 209 SPERVVQAIGELAKATGTSGLVAGQVVDIDSEGSNDVGLELLEYIHVHKTAALLEAAAVM 268
           SP R+V+AIGELAK+ G+ GLVAGQVVDI+SEG +DVGLE LE+IH+HKTAALLE A V+
Sbjct: 213 SPARIVRAIGELAKSIGSEGLVAGQVVDINSEGLSDVGLEQLEFIHLHKTAALLEGAVVL 272

Query: 269 GAIIGGGSNTEVEKVRKFARQIGLLFQVVDDILDVTKSSEELGKTAGKDLAADKATYPKL 328
           GAI+GGGS+ +VEK+RKFAR IGLLFQVVDDILDVTKSS+ELGKTAGKDL ADK TYPKL
Sbjct: 273 GAILGGGSDEDVEKLRKFARYIGLLFQVVDDILDVTKSSQELGKTAGKDLVADKVTYPKL 332

Query: 329 LGIEKSKEFAETLKREAGEALAGFDPAKAEPLIALSNYIAYRQN 373
           LGIEKSKEFAE L R+A E L+GFD  K+ PLIAL+NYIAYRQN
Sbjct: 333 LGIEKSKEFAEKLNRDAQEQLSGFDLNKSAPLIALANYIAYRQN 370

[Close]
BLAST of CmoCh07G007770 vs. TAIR10
Match: AT4G36810.1 (AT4G36810.1 geranylgeranyl pyrophosphate synthase 1)

HSP 1 Score: 452.6 bits (1163), Expect = 2.4e-127
Identity = 240/337 (71.22%), Postives = 278/337 (82.49%), Query Frame = 1 

Query: 39  SFSSKSIKKPSFNLRISAAQTRVETELRPVVEQNDQKGVFSFKDYMVQKANLVNQALNDA 98
           SF SK     S ++  S+  T+ E  LR       +   F F  Y++ KA LVN+AL+ A
Sbjct: 41  SFRSKRTVSSSSSIVSSSVVTK-EDNLR-----QSEPSSFDFMSYIITKAELVNKALDSA 100

Query: 99  VSLRDPLKIHEAMRYSLLAGGKRVRPVLCLAACELVGGSESAAMPAACATEMIHTMSLIH 158
           V LR+PLKIHEAMRYSLLAGGKRVRPVLC+AACELVGG ES AMPAACA EMIHTMSLIH
Sbjct: 101 VPLREPLKIHEAMRYSLLAGGKRVRPVLCIAACELVGGEESTAMPAACAVEMIHTMSLIH 160

Query: 159 DDLPCMDNDDLRRGKPTSHRMFGEDIAILAGDALLAFAFEHIAVATEAG-VSPERVVQAI 218
           DDLPCMDNDDLRRGKPT+H++FGED+A+LAGDALL+FAFEH+A AT +  VSP RVV+A+
Sbjct: 161 DDLPCMDNDDLRRGKPTNHKVFGEDVAVLAGDALLSFAFEHLASATSSDVVSPVRVVRAV 220

Query: 219 GELAKATGTSGLVAGQVVDIDSEG--SNDVGLELLEYIHVHKTAALLEAAAVMGAIIGGG 278
           GELAKA GT GLVAGQVVDI SEG   NDVGLE LE+IH+HKTAALLEA+AV+GAI+GGG
Sbjct: 221 GELAKAIGTEGLVAGQVVDISSEGLDLNDVGLEHLEFIHLHKTAALLEASAVLGAIVGGG 280

Query: 279 SNTEVEKVRKFARQIGLLFQVVDDILDVTKSSEELGKTAGKDLAADKATYPKLLGIEKSK 338
           S+ E+E++RKFAR IGLLFQVVDDILDVTKSS+ELGKTAGKDL ADK TYPK++G+EKS+
Sbjct: 281 SDDEIERLRKFARCIGLLFQVVDDILDVTKSSKELGKTAGKDLIADKLTYPKIMGLEKSR 340

Query: 339 EFAETLKREAGEALAGFDPAKAEPLIALSNYIAYRQN 373
           EFAE L REA + L GFD  K  PL+AL+NYIAYRQN
Sbjct: 341 EFAEKLNREARDQLLGFDSDKVAPLLALANYIAYRQN 371

[Close]
BLAST of CmoCh07G007770 vs. TAIR10
Match: AT2G18620.1 (AT2G18620.1 Terpenoid synthases superfamily protein)

HSP 1 Score: 439.9 bits (1130), Expect = 1.6e-123
Identity = 223/297 (75.08%), Postives = 260/297 (87.54%), Query Frame = 1 

Query: 78  FSFKDYMVQKANLVNQALNDAVSLRDPLKIHEAMRYSLLAGGKRVRPVLCLAACELVGGS 137
           F F  YM+ KAN VN+AL+ AVSLR+P+KIHEA+RYSLLA GKRVRPVLC+AACELVGG 
Sbjct: 53  FDFTSYMIGKANAVNEALDSAVSLREPIKIHEAIRYSLLARGKRVRPVLCIAACELVGGE 112

Query: 138 ESAAMPAACATEMIHTMSLIHDDLPCMDNDDLRRGKPTSHRMFGEDIAILAGDALLAFAF 197
           ES A+PAACA EMIHTMSLIHDDLPCMDNDDLRRGKPT+H++FGED+A+LAGDAL++FAF
Sbjct: 113 ESVALPAACAVEMIHTMSLIHDDLPCMDNDDLRRGKPTNHKVFGEDVAVLAGDALISFAF 172

Query: 198 EHIAVATEAGVSPERVVQAIGELAKATGTSGLVAGQVVDIDSEG--SNDVGLELLEYIHV 257
           EH+A +T   VSP RVV+AIGELAKA G+ GLVAGQVVD+ S G   NDVGLE+LE+IHV
Sbjct: 173 EHLATST--AVSPARVVRAIGELAKAIGSKGLVAGQVVDLTSGGMDQNDVGLEVLEFIHV 232

Query: 258 HKTAALLEAAAVMGAIIGGGSNTEVEKVRKFARQIGLLFQVVDDILDVTKSSEELGKTAG 317
           HKTA LLEAA V+GAI+GGGS+ EVEK+R+FAR IGLLFQVVDDILDVTKSSEELGKTAG
Sbjct: 233 HKTAVLLEAATVLGAIVGGGSDEEVEKLRRFARCIGLLFQVVDDILDVTKSSEELGKTAG 292

Query: 318 KDLAADKATYPKLLGIEKSKEFAETLKREAGEALAGFDPAKAEPLIALSNYIAYRQN 373
           KDL ADK TYPKL+G+EKSK+FA+ L  +A E L GFD ++ +PL+AL+NYIA RQN
Sbjct: 293 KDLIADKLTYPKLMGLEKSKDFADKLLSDAHEQLHGFDSSRVKPLLALANYIAKRQN 347

[Close]
BLAST of CmoCh07G007770 vs. TAIR10
Match: AT3G29430.1 (AT3G29430.1 Terpenoid synthases superfamily protein)

HSP 1 Score: 409.8 bits (1052), Expect = 1.7e-114
Identity = 214/341 (62.76%), Postives = 270/341 (79.18%), Query Frame = 1 

Query: 34  WNPLSSFSSKSIKKPSFNLRISAAQTRVETELRPVVEQNDQKGVFSFKDYMVQKANLVNQ 93
           +N LSSF++  ++K +      A  ++    + P  + ND+   F FK YM++KA  V+ 
Sbjct: 19  YNSLSSFNN--LQKRTVLSLSCALSSQGGDMIPPEGKSNDRNSAFDFKSYMIRKAESVSA 78

Query: 94  ALNDAVSLRDPLKIHEAMRYSLLAGGKRVRPVLCLAACELVGGSESAAMPAACATEMIHT 153
           ALN +V L++PL I EA+RYSLLAGGKRVRP+LC+AACELVGG E+ AM AACA EMIHT
Sbjct: 79  ALNVSVPLQEPLTIQEAVRYSLLAGGKRVRPLLCIAACELVGGDEATAMSAACAVEMIHT 138

Query: 154 MSLIHDDLPCMDNDDLRRGKPTSHRMFGEDIAILAGDALLAFAFEHIAVATEAGVSPERV 213
            SLIHDDLPCMD+ DLRRGKPT+H+ FGED+A+LAGDALLA AFEH+   +   V+PER+
Sbjct: 139 SSLIHDDLPCMDDADLRRGKPTNHKEFGEDMAVLAGDALLALAFEHMTFVSNGLVAPERM 198

Query: 214 VQAIGELAKATGTSGLVAGQVVDIDSEGSN--DVGLELLEYIHVHKTAALLEAAAVMGAI 273
           ++A+ ELAKA GT GLVAGQV D+ S+G N  DVGLE LE+IH+HKTAALLEAAAV+GAI
Sbjct: 199 IRAVMELAKAIGTKGLVAGQVTDLCSQGLNPDDVGLERLEFIHLHKTAALLEAAAVLGAI 258

Query: 274 IGGGSNTEVEKVRKFARQIGLLFQVVDDILDVTKSSEELGKTAGKDLAADKATYPKLLGI 333
           +GGG+  E+EK+RK+AR IGLLFQVVDDILDVT+S++ELGKTAGKD+ A K TYP+L+G+
Sbjct: 259 MGGGTEEEIEKLRKYARCIGLLFQVVDDILDVTESTKELGKTAGKDVMAGKLTYPRLIGL 318

Query: 334 EKSKEFAETLKREAGEALAGFDPAKAEPLIALSNYIAYRQN 373
           E+S+E AE L+REA E L GFD  KA PL+AL++YIA R N
Sbjct: 319 ERSREVAEKLRREAAEQLLGFDSDKAAPLVALASYIACRHN 357

[Close]
BLAST of CmoCh07G007770 vs. TAIR10
Match: AT3G14550.1 (AT3G14550.1 geranylgeranyl pyrophosphate synthase 3)

HSP 1 Score: 407.9 bits (1047), Expect = 6.6e-114
Identity = 213/335 (63.58%), Postives = 267/335 (79.70%), Query Frame = 1 

Query: 41  SSKSIKKPSFNLRISAAQTRVETELRPVVEQ-NDQKGVFSFKDYMVQKANLVNQALNDAV 100
           S KS+KK +     SA  ++   ++ P   + ND    F FK YM++KA  VN AL+ +V
Sbjct: 26  SVKSLKKRTGLSPSSALTSQGGRDMIPPEGKCNDHNSAFDFKLYMIRKAESVNAALDVSV 85

Query: 101 SLRDPLKIHEAMRYSLLAGGKRVRPVLCLAACELVGGSESAAMPAACATEMIHTMSLIHD 160
            LR+PL + EA+RYSLLAGGKRVRP+LC+A CELVGG E+ AM AACA EMIHT SLIHD
Sbjct: 86  PLREPLTVQEAVRYSLLAGGKRVRPLLCIAVCELVGGDEATAMSAACAVEMIHTSSLIHD 145

Query: 161 DLPCMDNDDLRRGKPTSHRMFGEDIAILAGDALLAFAFEHIAVATEAGVSPERVVQAIGE 220
           DLPCMDN DLRRGKPT+H+++GED+A+LAGDALLA AFEH+   +   V+PER+++A+ E
Sbjct: 146 DLPCMDNADLRRGKPTNHKVYGEDMAVLAGDALLALAFEHMTFVSSGLVAPERMIRAVVE 205

Query: 221 LAKATGTSGLVAGQVVDIDSEGSN--DVGLELLEYIHVHKTAALLEAAAVMGAIIGGGSN 280
           LA+A GT+GLVAGQ++D+ SE  N   VGLE LE+IH+HKTAALLEAAAV+G I+GGG+ 
Sbjct: 206 LARAIGTTGLVAGQMIDLASERLNPDKVGLEHLEFIHLHKTAALLEAAAVLGVIMGGGTE 265

Query: 281 TEVEKVRKFARQIGLLFQVVDDILDVTKSSEELGKTAGKDLAADKATYPKLLGIEKSKEF 340
            E+EK+RK+AR IGLLFQVVDDILDVTKS+EELGKTAGKD+ A K TYP+L+G+E+SKE 
Sbjct: 266 EEIEKLRKYARCIGLLFQVVDDILDVTKSTEELGKTAGKDVMAGKLTYPRLIGLERSKEV 325

Query: 341 AETLKREAGEALAGFDPAKAEPLIALSNYIAYRQN 373
           AE L+REA E L GFDP+KA PL+AL++YIA R N
Sbjct: 326 AEKLRREAEEQLLGFDPSKAAPLVALASYIACRHN 360

[Close]
BLAST of CmoCh07G007770 vs. TAIR10
Match: AT3G14530.1 (AT3G14530.1 Terpenoid synthases superfamily protein)

HSP 1 Score: 407.9 bits (1047), Expect = 6.6e-114
Identity = 214/336 (63.69%), Postives = 269/336 (80.06%), Query Frame = 1 

Query: 41  SSKSIKKPSFNLRISAAQTRVETE--LRPVVEQNDQKGVFSFKDYMVQKANLVNQALNDA 100
           S KS++K +  L +S+A T  +    ++P  + ND    F FK YM++KA  VN AL+ +
Sbjct: 26  SVKSLRKRTV-LSLSSALTSQDAGHMIQPEGKSNDNNSAFDFKLYMIRKAESVNAALDVS 85

Query: 101 VSLRDPLKIHEAMRYSLLAGGKRVRPVLCLAACELVGGSESAAMPAACATEMIHTMSLIH 160
           V L  PL I EA+RYSLLAGGKRVRP+LC+AACELVGG E+ AM AACA EMIHT SLIH
Sbjct: 86  VPLLKPLTIQEAVRYSLLAGGKRVRPLLCIAACELVGGDEATAMSAACAVEMIHTSSLIH 145

Query: 161 DDLPCMDNDDLRRGKPTSHRMFGEDIAILAGDALLAFAFEHIAVATEAGVSPERVVQAIG 220
           DDLPCMDN DLRRGKPT+H+++GED+A+LAGDALLA AFEH+ V +   V+PE++++A+ 
Sbjct: 146 DDLPCMDNADLRRGKPTNHKVYGEDMAVLAGDALLALAFEHMTVVSSGLVAPEKMIRAVV 205

Query: 221 ELAKATGTSGLVAGQVVDIDSEGSN--DVGLELLEYIHVHKTAALLEAAAVMGAIIGGGS 280
           ELA+A GT+GLVAGQ++D+ SE  N   VGLE LE+IH+HKTAALLEAAAV+G I+GGG+
Sbjct: 206 ELARAIGTTGLVAGQMIDLASERLNPDKVGLEHLEFIHLHKTAALLEAAAVLGVIMGGGT 265

Query: 281 NTEVEKVRKFARQIGLLFQVVDDILDVTKSSEELGKTAGKDLAADKATYPKLLGIEKSKE 340
             E+EK+RK+AR IGLLFQVVDDILDVTKS+EELGKTAGKD+ A K TYP+L+G+E S+E
Sbjct: 266 EQEIEKLRKYARCIGLLFQVVDDILDVTKSTEELGKTAGKDVMAGKLTYPRLIGLEGSRE 325

Query: 341 FAETLKREAGEALAGFDPAKAEPLIALSNYIAYRQN 373
            AE L+REA E L GFDP+KA PL+AL++YIA R N
Sbjct: 326 VAEKLRREAEEQLLGFDPSKAAPLVALASYIACRHN 360

[Close]
BLAST of CmoCh07G007770 vs. NCBI nr
Match: gi|659079248|ref|XP_008440155.1| (PREDICTED: geranylgeranyl pyrophosphate synthase 7, chloroplastic-like [Cucumis melo])

HSP 1 Score: 556.6 bits (1433), Expect = 3.3e-155
Identity = 297/374 (79.41%), Postives = 329/374 (87.97%), Query Frame = 1 

Query: 2   SSVSFANPLIQSNSVLNNSQKFNQKSFSFLHPW-NPLSSF-SSKSIKKPSFNLRISAAQT 61
           SS +F NPLI S+SV  NS K   KS   LHP  +P+S    SKSIK PS    +SA  T
Sbjct: 3   SSFNFTNPLIHSSSVFTNSHKSKSKSSFLLHPLTSPISLLIPSKSIKTPSSRRPLSAVLT 62

Query: 62  RVETELRPVVEQND-QKGVFSFKDYMVQKANLVNQALNDAVSLRDPLKIHEAMRYSLLAG 121
             +TEL+P +EQND Q   F FKDYM+QKANLVNQALNDAVSL+DPLKIHEAMRYSLLAG
Sbjct: 63  TEQTELQPKLEQNDDQNPSFRFKDYMIQKANLVNQALNDAVSLQDPLKIHEAMRYSLLAG 122

Query: 122 GKRVRPVLCLAACELVGGSESAAMPAACATEMIHTMSLIHDDLPCMDNDDLRRGKPTSHR 181
           GKRVRPVLCLAAC+LVGG ESAA+PAAC+ EMIHTMSLIHDDLPCMDNDDLRRGKPT+HR
Sbjct: 123 GKRVRPVLCLAACDLVGGPESAAIPAACSIEMIHTMSLIHDDLPCMDNDDLRRGKPTNHR 182

Query: 182 MFGEDIAILAGDALLAFAFEHIAVA-TEAGVSPERVVQAIGELAKATGTSGLVAGQVVDI 241
           MFGED+A+LAGDALL+FAFEH+A A T AGVSPERVV+AIGELAKATG+ GLVAGQVVDI
Sbjct: 183 MFGEDVAVLAGDALLSFAFEHMAAAVTAAGVSPERVVRAIGELAKATGSGGLVAGQVVDI 242

Query: 242 DSEGSNDVGLELLEYIHVHKTAALLEAAAVMGAIIGGGSNTEVEKVRKFARQIGLLFQVV 301
           +SEG+NDVGLELLEYIHVHKTAALLEAAAV+GAI+GGGS  E+EK+R FAR+IGLLFQVV
Sbjct: 243 ESEGANDVGLELLEYIHVHKTAALLEAAAVIGAIVGGGSEEEIEKLRIFARRIGLLFQVV 302

Query: 302 DDILDVTKSSEELGKTAGKDLAADKATYPKLLGIEKSKEFAETLKREAGEALAGFDPAKA 361
           DDILDVTKS+EELGKTAGKD+AA+KATYPKL+GIEKSKEFAETL+ EA EALAGFDP KA
Sbjct: 303 DDILDVTKSTEELGKTAGKDVAAEKATYPKLMGIEKSKEFAETLREEAVEALAGFDPVKA 362

Query: 362 EPLIALSNYIAYRQ 372
           EPLIALSNYIAYRQ
Sbjct: 363 EPLIALSNYIAYRQ 376

[Close]
BLAST of CmoCh07G007770 vs. NCBI nr
Match: gi|449448558|ref|XP_004142033.1| (PREDICTED: geranylgeranyl pyrophosphate synthase 7, chloroplastic [Cucumis sativus])

HSP 1 Score: 554.7 bits (1428), Expect = 1.2e-154
Identity = 296/373 (79.36%), Postives = 330/373 (88.47%), Query Frame = 1 

Query: 2   SSVSFANPLIQSNSVLNNSQKFNQKSFSFLHPW-NPLSSF-SSKSIKKPSFNLRISAAQT 61
           SS++F NPLIQS+SV  NS KF    F  LHP  +P+S   SSKS K  S    ISAA T
Sbjct: 3   SSLNFTNPLIQSSSVFTNSHKFKSNPFFLLHPLTSPISLLISSKSTKNSSSRRPISAALT 62

Query: 62  RVETELRPVVEQND-QKGVFSFKDYMVQKANLVNQALNDAVSLRDPLKIHEAMRYSLLAG 121
             +TEL P +EQND Q   F F+DYM+QK+NLVNQAL++AVSL+DPLKIHEAMRYSLLAG
Sbjct: 63  TEQTELHPQLEQNDVQNPPFRFRDYMIQKSNLVNQALDEAVSLQDPLKIHEAMRYSLLAG 122

Query: 122 GKRVRPVLCLAACELVGGSESAAMPAACATEMIHTMSLIHDDLPCMDNDDLRRGKPTSHR 181
           GKRVRPVLCLAAC+LVGGSESAA+PAAC+ EMIHTMSLIHDDLPCMDNDDLRRGKPT+HR
Sbjct: 123 GKRVRPVLCLAACDLVGGSESAAIPAACSIEMIHTMSLIHDDLPCMDNDDLRRGKPTNHR 182

Query: 182 MFGEDIAILAGDALLAFAFEHIAVATEAGVSPERVVQAIGELAKATGTSGLVAGQVVDID 241
           MFGED+A+LAGDALL+FAFEH+A AT+A VSPERVV+AIGELAKATGT GLVAGQVVDID
Sbjct: 183 MFGEDVAVLAGDALLSFAFEHMA-ATKAEVSPERVVRAIGELAKATGTGGLVAGQVVDID 242

Query: 242 SEGSNDVGLELLEYIHVHKTAALLEAAAVMGAIIGGGSNTEVEKVRKFARQIGLLFQVVD 301
           SEG++DVGLELLEYIHVHKTAALLEAAAVMGAI+GGGS  E+EK+R FAR+IGLLFQVVD
Sbjct: 243 SEGADDVGLELLEYIHVHKTAALLEAAAVMGAIVGGGSEEEIEKLRIFARRIGLLFQVVD 302

Query: 302 DILDVTKSSEELGKTAGKDLAADKATYPKLLGIEKSKEFAETLKREAGEALAGFDPAKAE 361
           DILDVTKS+EELGKTAGKD+AA+KATYPKL+GIEKSKEFAE L+ EA EALAGFDP KAE
Sbjct: 303 DILDVTKSTEELGKTAGKDVAAEKATYPKLMGIEKSKEFAEKLREEAIEALAGFDPVKAE 362

Query: 362 PLIALSNYIAYRQ 372
           PLIALSNYIAYRQ
Sbjct: 363 PLIALSNYIAYRQ 374

[Close]
BLAST of CmoCh07G007770 vs. NCBI nr
Match: gi|590703979|ref|XP_007047029.1| (Geranylgeranyl pyrophosphate synthase 1 [Theobroma cacao])

HSP 1 Score: 487.3 bits (1253), Expect = 2.4e-134
Identity = 257/362 (70.99%), Postives = 294/362 (81.22%), Query Frame = 1 

Query: 11  IQSNSVLNNSQKFNQKSFSFLHPWNPLSSFSSKSIKKPSFNLRISAAQTRVETELRPVVE 70
           +QS S+ N + +          P+NPL + SS + KK S     S +    + +     E
Sbjct: 10  VQSYSIFNQASRSKSIPLPRSFPFNPLKNLSSSTPKKRSLTSLPSISAVLTKEDTIKEEE 69

Query: 71  QNDQKGVFSFKDYMVQKANLVNQALNDAVSLRDPLKIHEAMRYSLLAGGKRVRPVLCLAA 130
           Q+ QK  F FK YMVQKA+ VNQAL+ AVSLRDP+KIHEAMRYSLLAGGKRVRP LCLAA
Sbjct: 70  QDPQKPSFDFKSYMVQKASTVNQALDSAVSLRDPVKIHEAMRYSLLAGGKRVRPALCLAA 129

Query: 131 CELVGGSESAAMPAACATEMIHTMSLIHDDLPCMDNDDLRRGKPTSHRMFGEDIAILAGD 190
           CELVGG ES AMPAACA EMIHTMSLIHDDLPCMDNDDLRRGKPT+H++FGEDIA+LAGD
Sbjct: 130 CELVGGQESMAMPAACAVEMIHTMSLIHDDLPCMDNDDLRRGKPTNHKVFGEDIAVLAGD 189

Query: 191 ALLAFAFEHIAVATEAGVSPERVVQAIGELAKATGTSGLVAGQVVDIDSEGSNDVGLELL 250
           ALLAFAFEHIAV+T  GV+P RVV+A+GELAKA GT GLVAGQVVDI+SEG  DVGL+ L
Sbjct: 190 ALLAFAFEHIAVST-VGVTPGRVVKAVGELAKAIGTEGLVAGQVVDINSEGLIDVGLDHL 249

Query: 251 EYIHVHKTAALLEAAAVMGAIIGGGSNTEVEKVRKFARQIGLLFQVVDDILDVTKSSEEL 310
           E+IHVHKTA LLEAA V+GAI+GGG + +VEK+RKFAR IGLLFQVVDDILDVTKSS++L
Sbjct: 250 EFIHVHKTAVLLEAAVVLGAILGGGCDEDVEKLRKFARYIGLLFQVVDDILDVTKSSKDL 309

Query: 311 GKTAGKDLAADKATYPKLLGIEKSKEFAETLKREAGEALAGFDPAKAEPLIALSNYIAYR 370
           GKTAGKDL ADK TYPKL+GIEKS+EFAE LK +A E L GFDP KA PL+AL+NYIAYR
Sbjct: 310 GKTAGKDLVADKVTYPKLMGIEKSREFAEKLKSDALELLQGFDPEKAAPLVALANYIAYR 369

Query: 371 QN 373
           QN
Sbjct: 370 QN 370

[Close]
BLAST of CmoCh07G007770 vs. NCBI nr
Match: gi|823192258|ref|XP_012491701.1| (PREDICTED: geranylgeranyl pyrophosphate synthase, chloroplastic-like [Gossypium raimondii])

HSP 1 Score: 486.1 bits (1250), Expect = 5.4e-134
Identity = 262/364 (71.98%), Postives = 297/364 (81.59%), Query Frame = 1 

Query: 11  IQSNSVLNNSQKFNQKSFSFLH--PWNPLSSFSSKSIKKPSFNLRISAAQTRVETELRPV 70
           +QS S+ N     + KSF      P+NPL++ SS ++KK +F+   S      + ++   
Sbjct: 10  VQSYSMPNQVSSIS-KSFPLPRSLPFNPLTTQSSSTLKKVNFSSLPSITSVLTKEDIVKE 69

Query: 71  VEQNDQKGVFSFKDYMVQKANLVNQALNDAVSLRDPLKIHEAMRYSLLAGGKRVRPVLCL 130
            EQ+ QK  F F  YM+QKA+ VNQAL  AVSLRDP+KIHEAMRYSLLAGGKRVRPVLCL
Sbjct: 70  EEQDPQKSSFDFNSYMLQKASAVNQALESAVSLRDPVKIHEAMRYSLLAGGKRVRPVLCL 129

Query: 131 AACELVGGSESAAMPAACATEMIHTMSLIHDDLPCMDNDDLRRGKPTSHRMFGEDIAILA 190
           AAC LVGG ES AMPAACA EMIHTMSLIHDDLPCMDNDDLRRGKPT+H++FGEDIA+LA
Sbjct: 130 AACALVGGRESMAMPAACAVEMIHTMSLIHDDLPCMDNDDLRRGKPTNHKVFGEDIAVLA 189

Query: 191 GDALLAFAFEHIAVATEAGVSPERVVQAIGELAKATGTSGLVAGQVVDIDSEGSNDVGLE 250
           GDALLAFAFEHIAV+T  GV P R+V+AIGELAKA G  GLVAGQVVDI SEG  DVGL+
Sbjct: 190 GDALLAFAFEHIAVST-VGVPPSRIVRAIGELAKAIGGEGLVAGQVVDIISEGLTDVGLD 249

Query: 251 LLEYIHVHKTAALLEAAAVMGAIIGGGSNTEVEKVRKFARQIGLLFQVVDDILDVTKSSE 310
            LE+IHVHKTAALLEAA V+GAI+GGG + EVEK+RKFAR IGLLFQVVDDILDVTKSS+
Sbjct: 250 HLEFIHVHKTAALLEAAVVLGAIVGGGCDEEVEKLRKFARCIGLLFQVVDDILDVTKSSK 309

Query: 311 ELGKTAGKDLAADKATYPKLLGIEKSKEFAETLKREAGEALAGFDPAKAEPLIALSNYIA 370
           ELGKTAGKDL ADK TYPKL+GI+KSKEFAE LK +A E L GFDPAKA PLIAL+NYIA
Sbjct: 310 ELGKTAGKDLVADKVTYPKLMGIQKSKEFAEKLKTDAIELLQGFDPAKAAPLIALANYIA 369

Query: 371 YRQN 373
           YRQN
Sbjct: 370 YRQN 371

[Close]
BLAST of CmoCh07G007770 vs. NCBI nr
Match: gi|657948668|ref|XP_008338839.1| (PREDICTED: geranylgeranyl pyrophosphate synthase, chloroplastic-like [Malus domestica])

HSP 1 Score: 484.6 bits (1246), Expect = 1.6e-133
Identity = 256/365 (70.14%), Postives = 306/365 (83.84%), Query Frame = 1 

Query: 12  QSNSVLNNSQKF-NQKSFSFLHPWN---PLSSFSSKSIKKPSFNLRISAAQTRVETELRP 71
           Q+ S+LN + +  + +SF  LHP     P+SS ++   ++P+    +SA  T+ ET L  
Sbjct: 13  QTCSMLNQAGRSRSSQSFHLLHPLKTTIPISS-AAPHRRRPTSPFAVSAVLTKQET-LTE 72

Query: 72  VVEQNDQKGVFSFKDYMVQKANLVNQALNDAVSLRDPLKIHEAMRYSLLAGGKRVRPVLC 131
             ++  +K  F+FK YM+Q+A  VNQAL+ AVSLRDP+ IHEAMRYSLLAGGKRVRPVLC
Sbjct: 73  EFDEESRKPTFNFKSYMLQRAESVNQALDAAVSLRDPITIHEAMRYSLLAGGKRVRPVLC 132

Query: 132 LAACELVGGSESAAMPAACATEMIHTMSLIHDDLPCMDNDDLRRGKPTSHRMFGEDIAIL 191
           LAACELVGGSES AMPAACA EMIHTMSLIHDDLPCMDNDDLRRGKPT+H++FGED+A+L
Sbjct: 133 LAACELVGGSESMAMPAACAVEMIHTMSLIHDDLPCMDNDDLRRGKPTNHKVFGEDVAVL 192

Query: 192 AGDALLAFAFEHIAVATEAGVSPERVVQAIGELAKATGTSGLVAGQVVDIDSEGSNDVGL 251
           AGDALLAFAFEHI+++T  GVSP R+V+A+GELAK+ G+ GLVAGQVVDIDSEG +DVGL
Sbjct: 193 AGDALLAFAFEHISLST-VGVSPARIVRAVGELAKSIGSEGLVAGQVVDIDSEGLSDVGL 252

Query: 252 ELLEYIHVHKTAALLEAAAVMGAIIGGGSNTEVEKVRKFARQIGLLFQVVDDILDVTKSS 311
           E LEYIH+HKTAALLE + V+G+I+GGGS++E+EK+R FAR IGLLFQVVDDILDVTKSS
Sbjct: 253 EQLEYIHLHKTAALLECSVVLGSILGGGSDSEIEKLRTFARYIGLLFQVVDDILDVTKSS 312

Query: 312 EELGKTAGKDLAADKATYPKLLGIEKSKEFAETLKREAGEALAGFDPAKAEPLIALSNYI 371
           +ELGKTAGKDL ADK TYPKLLGIEKS+EFAE L ++A E LAGFDP KA PLIAL+NYI
Sbjct: 313 QELGKTAGKDLVADKVTYPKLLGIEKSREFAEKLNKDAKEQLAGFDPEKAAPLIALANYI 372

Query: 372 AYRQN 373
           AYRQN
Sbjct: 373 AYRQN 374

The following BLAST results are available for this feature:
BLAST of CmoCh07G007770 vs. Swiss-Prot
Match NameE-valueIdentityDescription
GGPPS_HEVBR8.7e-13267.55Geranylgeranyl pyrophosphate synthase, chloroplastic OS=Hevea brasiliensis GN=GG... [more]
GGPPS_CATRO1.7e-12769.45Geranylgeranyl pyrophosphate synthase, chloroplastic OS=Catharanthus roseus GN=G... [more]
GGPP1_ARATH4.2e-12671.22Heterodimeric geranylgeranyl pyrophosphate synthase large subunit 1, chloroplast... [more]
GGPPS_SINAL7.4e-12369.14Geranylgeranyl pyrophosphate synthase, chloroplastic/chromoplastic OS=Sinapis al... [more]
GGPP7_ARATH2.8e-12275.08Geranylgeranyl pyrophosphate synthase 7, chloroplastic OS=Arabidopsis thaliana G... [more]
BLAST of CmoCh07G007770 vs. TrEMBL
Match NameE-valueIdentityDescription
A0A0A0KFZ6_CUCSA8.7e-15579.36Uncharacterized protein OS=Cucumis sativus GN=Csa_6G487640 PE=3 SV=1[more]
A0A061DHH0_THECC1.7e-13470.99Geranylgeranyl pyrophosphate synthase 1 OS=Theobroma cacao GN=TCM_000455 PE=3 SV... [more]
A0A0D2SNN1_GOSRA3.8e-13471.98Uncharacterized protein OS=Gossypium raimondii GN=B456_007G206700 PE=3 SV=1[more]
A0A0G2QS78_MALDO2.5e-13370.05Geranylgeranyl diphosphate synthase OS=Malus domestica GN=GGPPS-1 PE=2 SV=1[more]
D6Q1T4_MEDSA6.1e-13272.38Geranylgeranyl-diphosphate synthase OS=Medicago sativa GN=GGPPS PE=2 SV=1[more]
BLAST of CmoCh07G007770 vs. TAIR10
Match NameE-valueIdentityDescription
AT4G36810.12.4e-12771.22 geranylgeranyl pyrophosphate synthase 1[more]
AT2G18620.11.6e-12375.08 Terpenoid synthases superfamily protein[more]
AT3G29430.11.7e-11462.76 Terpenoid synthases superfamily protein[more]
AT3G14550.16.6e-11463.58 geranylgeranyl pyrophosphate synthase 3[more]
AT3G14530.16.6e-11463.69 Terpenoid synthases superfamily protein[more]
BLAST of CmoCh07G007770 vs. NCBI nr
Match NameE-valueIdentityDescription
gi|659079248|ref|XP_008440155.1|3.3e-15579.41PREDICTED: geranylgeranyl pyrophosphate synthase 7, chloroplastic-like [Cucumis ... [more]
gi|449448558|ref|XP_004142033.1|1.2e-15479.36PREDICTED: geranylgeranyl pyrophosphate synthase 7, chloroplastic [Cucumis sativ... [more]
gi|590703979|ref|XP_007047029.1|2.4e-13470.99Geranylgeranyl pyrophosphate synthase 1 [Theobroma cacao][more]
gi|823192258|ref|XP_012491701.1|5.4e-13471.98PREDICTED: geranylgeranyl pyrophosphate synthase, chloroplastic-like [Gossypium ... [more]
gi|657948668|ref|XP_008338839.1|1.6e-13370.14PREDICTED: geranylgeranyl pyrophosphate synthase, chloroplastic-like [Malus dome... [more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR000092Polyprenyl_synt
IPR008949Isoprenoid_synthase_dom_sf
IPR017446Polyprenyl synthetase-related
Vocabulary: Biological Process
TermDefinition
GO:0008299isoprenoid biosynthetic process
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008299 isoprenoid biosynthetic process
biological_process GO:0006694 steroid biosynthetic process
biological_process GO:0016114 terpenoid biosynthetic process
cellular_component GO:0005575 cellular_component
cellular_component GO:0005622 intracellular
molecular_function GO:0016740 transferase activity
molecular_function GO:0004161 dimethylallyltranstransferase activity
molecular_function GO:0004337 geranyltranstransferase activity
molecular_function GO:0036422 heptaprenyl diphosphate synthase activity
molecular_function GO:0004311 farnesyltranstransferase activity
molecular_function GO:0003674 molecular_function
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmoCh07G007770.1CmoCh07G007770.1mRNA


InterPro
Analysis Name: InterPro Annotations of Cucurbita moschata
Date Performed: 2017-05-19
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000092Polyprenyl synthetasePFAMPF00348polyprenyl_syntcoord: 107..343
score: 5.4
IPR000092Polyprenyl synthetasePROSITEPS00444POLYPRENYL_SYNTHASE_2coord: 290..302
scor
IPR000092Polyprenyl synthetasePROSITEPS00723POLYPRENYL_SYNTHASE_1coord: 156..172
scor
IPR008949Isoprenoid synthase domainGENE3DG3DSA:1.10.600.10coord: 78..370
score: 1.2E
IPR008949Isoprenoid synthase domainunknownSSF48576Terpenoid synthasescoord: 80..352
score: 1.52
IPR017446Polyprenyl synthetase-relatedPANTHERPTHR12001GERANYLGERANYL PYROPHOSPHATE SYNTHASEcoord: 23..372
score: 8.4E
NoneNo IPR availablePANTHERPTHR12001:SF52GERANYLGERANYL PYROPHOSPHATE SYNTHASE 10, MITOCHONDRIAL-RELATEDcoord: 23..372
score: 8.4E