CmoCh04G009900 (gene) Cucurbita moschata (Rifu) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	CmoCh04G009900
Type	gene
Organism	Cucurbita moschata (Cucurbita moschata (Rifu))
Description	Calcium-binding EF-hand family protein, putative
Location	Cmo_Chr04 : 4989021 .. 4989739 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGATGGTGGAAATGAGAAGGCATTTGTTCCCATATATATATATATATCCCAAGGCCTAAAGGACGCTCCATGTGGGCTACCATGCAGCATGTCTTCCATCCCTACTCGCTGCTGTAAACATGCTTTCAACTTTGTCCTTACCAAGATTCCTCATTGTCAATGCTTTTATAGATAAGGATTATGAATGTTTTAAGGTTTTCTTTTCTACACTTTTATTCATATTTCTCATACAATAATAAAAAGTAAAAATAAAAATCATTTTCCATGTTAAACTGAAGATACATGCATAATAATGCTGCCTTTTTTTTTCTTTTTATACTTATTCTCTGGCACTTATCTGTTTGTAAAACAAAATTACACGTCTATATAATACACACAACACAACACCATGACACCCCAAACAAAACTCGTGAAAATCAAAAACCCATTTCAGGTTTGACGGAGATGCCTCGCAAAACCAAAATGTACCATCAGAGGCCCAAGATATCAAGACAGCAATTAAGGGTAATCTTCAGAAACCACGACCGAGATGGTGACGGCCGCCTGAGCTCCGCCGAGCTGACCAAAGCCTTCCACTACATCGGTGCCCTCCTGCCATCATTCAGAGCTTGCCACGCTCTTCTTTACACCGATTCTGACAGAGACGGATTCATCGATGAGAAGGATTTCGAGCAGCTCATTGATTATGCCGAGAAACGCCAATACACCGTCATTTAA

mRNA sequence

ATGGATGGTGGAAATGAGAAGGCATTTGTTCCCATATATATATATATATCCCAAGGCCTAAAGGACGCTCCATGTGGGCTACCATGCAGCATGTCTTCCATCCCTACTCGCTGCTGTTTGACGGAGATGCCTCGCAAAACCAAAATGTACCATCAGAGGCCCAAGATATCAAGACAGCAATTAAGGGTAATCTTCAGAAACCACGACCGAGATGGTGACGGCCGCCTGAGCTCCGCCGAGCTGACCAAAGCCTTCCACTACATCGGTGCCCTCCTGCCATCATTCAGAGCTTGCCACGCTCTTCTTTACACCGATTCTGACAGAGACGGATTCATCGATGAGAAGGATTTCGAGCAGCTCATTGATTATGCCGAGAAACGCCAATACACCGTCATTTAA

Coding sequence (CDS)

ATGGATGGTGGAAATGAGAAGGCATTTGTTCCCATATATATATATATATCCCAAGGCCTAAAGGACGCTCCATGTGGGCTACCATGCAGCATGTCTTCCATCCCTACTCGCTGCTGTTTGACGGAGATGCCTCGCAAAACCAAAATGTACCATCAGAGGCCCAAGATATCAAGACAGCAATTAAGGGTAATCTTCAGAAACCACGACCGAGATGGTGACGGCCGCCTGAGCTCCGCCGAGCTGACCAAAGCCTTCCACTACATCGGTGCCCTCCTGCCATCATTCAGAGCTTGCCACGCTCTTCTTTACACCGATTCTGACAGAGACGGATTCATCGATGAGAAGGATTTCGAGCAGCTCATTGATTATGCCGAGAAACGCCAATACACCGTCATTTAA

Homology

[Close]

BLAST of CmoCh04G009900 vs. TrEMBL
Match: A0A0A0KZR6_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_4G639730 PE=4 SV=1)

HSP 1 Score: 131.7 bits (330), Expect = 6.4e-28
Identity = 62/90 (68.89%), Postives = 76/90 (84.44%), Query Frame = 1


Query: 43  MPRKTKMYHQRPKISRQQLRVIFRNHDRDGDGRLSSAELTKAFHYIGALLPSFRACHALL 102
           M RK+K Y+ R K+SR+QL +IFRNHD D DGRLS+ ELT+AF YIGA LP++RA HALL
Sbjct: 1   MSRKSKNYYPRAKLSREQLLLIFRNHDSDKDGRLSTTELTEAFRYIGAFLPTYRARHALL 60

Query: 103 YTDSDRDGFIDEKDFEQLIDYAEKRQYTVI 133
           + DSDRDGFI EKDFE L++YAE+RQYT+I
Sbjct: 61  HADSDRDGFIGEKDFESLVNYAEQRQYTII 90

[Close]

BLAST of CmoCh04G009900 vs. TrEMBL
Match: A5AWP8_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_023174 PE=4 SV=1)

HSP 1 Score: 82.0 bits (201), Expect = 5.9e-13
Identity = 38/84 (45.24%), Postives = 57/84 (67.86%), Query Frame = 1


Query: 48  KMYHQRPKISRQQLRVIFRNHDRDGDGRLSSAELTKAFHYIGALLPSFRACHALLYTDSD 107
           K    +  +  QQL+ IF+ HD +GDG+L+ AEL +AF ++GAL+P +RA  AL + D+D
Sbjct: 7   KTIPSKSPLPEQQLKKIFKQHDTNGDGQLNKAELKQAFQHLGALIPGWRAHRALHHADTD 66

Query: 108 RDGFIDEKDFEQLIDYAEKRQYTV 132
            DG I EK+ + L++YA K  YT+
Sbjct: 67  GDGCISEKEMKDLVEYAAKFGYTI 90

[Close]

BLAST of CmoCh04G009900 vs. TrEMBL
Match: A0A0A0LRC1_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G358870 PE=4 SV=1)

HSP 1 Score: 81.3 bits (199), Expect = 1.0e-12
Identity = 35/77 (45.45%), Postives = 57/77 (74.03%), Query Frame = 1


Query: 53  RPKISRQQLRVIFRNHDRDGDGRLSSAELTKAFHYIGALLPSFRACHALLYTDSDRDGFI 112
           R ++SR+ ++ IFR HD DGDGRLS  E+ KAF   G++LP ++A + + + D + DGFI
Sbjct: 7   RLQLSREDIKEIFREHDIDGDGRLSMNEMVKAFGCFGSILPFYKAHYGMAHADENGDGFI 66

Query: 113 DEKDFEQLIDYAEKRQY 130
           +E++ ++L+DYA+K Q+
Sbjct: 67  EEEELDKLVDYAQKFQH 83

[Close]

BLAST of CmoCh04G009900 vs. TrEMBL
Match: A0A0A0KMQ1_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G067410 PE=4 SV=1)

HSP 1 Score: 78.2 bits (191), Expect = 8.5e-12
Identity = 31/71 (43.66%), Postives = 53/71 (74.65%), Query Frame = 1


Query: 56  ISRQQLRVIFRNHDRDGDGRLSSAELTKAFHYIGALLPSFRACHALLYTDSDRDGFIDEK 115
           +SR++++ IF+ HD + DG L+  ELTKAF ++G++ P ++AC+ ++Y D++ DG I E 
Sbjct: 96  LSRERIKEIFQYHDNNNDGFLNRMELTKAFAFLGSMFPFYKACYGMVYADANEDGLISEA 155

Query: 116 DFEQLIDYAEK 127
           + ++LIDYA K
Sbjct: 156 ELDKLIDYASK 166

[Close]

BLAST of CmoCh04G009900 vs. TrEMBL
Match: A0A0A0KIY4_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G068170 PE=4 SV=1)

HSP 1 Score: 77.8 bits (190), Expect = 1.1e-11
Identity = 32/72 (44.44%), Postives = 53/72 (73.61%), Query Frame = 1


Query: 55  KISRQQLRVIFRNHDRDGDGRLSSAELTKAFHYIGALLPSFRACHALLYTDSDRDGFIDE 114
           + SR+Q++ IF+ HD D DG L+  E+TKAF ++G +LP ++A H ++Y D+++DG I +
Sbjct: 100 QFSREQIKEIFQYHDSDNDGFLNLREVTKAFAFLGTILPLYKAYHGMVYADANKDGLISK 159

Query: 115 KDFEQLIDYAEK 127
            + E+L+DYA K
Sbjct: 160 DELEKLVDYAYK 171

[Close]

BLAST of CmoCh04G009900 vs. TAIR10
Match: AT1G76650.1 (AT1G76650.1 calmodulin-like 38)

HSP 1 Score: 47.8 bits (112), Expect = 6.2e-06
Identity = 24/70 (34.29%), Postives = 40/70 (57.14%), Query Frame = 1


Query: 59  QQLRVIFRNHDRDGDGRLSSAELTKAFHYIGALLPSFRACHALLYTDSDRDGFIDEKDFE 118
           ++L  +F   D + DGR+S  EL K+F  +G  L    A  A+  +D+D DG +D ++F 
Sbjct: 42  RELEAVFSYMDANRDGRISPEELQKSFMTLGEQLSDEEAVAAVRLSDTDGDGMLDFEEFS 101

Query: 119 QLIDYAEKRQ 129
           QLI   ++ +
Sbjct: 102 QLIKVDDEEE 111

[Close]

BLAST of CmoCh04G009900 vs. NCBI nr
Match: gi|700200027|gb|KGN55185.1| (hypothetical protein Csa_4G639730 [Cucumis sativus])

HSP 1 Score: 131.7 bits (330), Expect = 9.2e-28
Identity = 62/90 (68.89%), Postives = 76/90 (84.44%), Query Frame = 1


Query: 43  MPRKTKMYHQRPKISRQQLRVIFRNHDRDGDGRLSSAELTKAFHYIGALLPSFRACHALL 102
           M RK+K Y+ R K+SR+QL +IFRNHD D DGRLS+ ELT+AF YIGA LP++RA HALL
Sbjct: 1   MSRKSKNYYPRAKLSREQLLLIFRNHDSDKDGRLSTTELTEAFRYIGAFLPTYRARHALL 60

Query: 103 YTDSDRDGFIDEKDFEQLIDYAEKRQYTVI 133
           + DSDRDGFI EKDFE L++YAE+RQYT+I
Sbjct: 61  HADSDRDGFIGEKDFESLVNYAEQRQYTII 90

[Close]

BLAST of CmoCh04G009900 vs. NCBI nr
Match: gi|147866710|emb|CAN78405.1| (hypothetical protein VITISV_023174 [Vitis vinifera])

HSP 1 Score: 82.0 bits (201), Expect = 8.4e-13
Identity = 38/84 (45.24%), Postives = 57/84 (67.86%), Query Frame = 1


Query: 48  KMYHQRPKISRQQLRVIFRNHDRDGDGRLSSAELTKAFHYIGALLPSFRACHALLYTDSD 107
           K    +  +  QQL+ IF+ HD +GDG+L+ AEL +AF ++GAL+P +RA  AL + D+D
Sbjct: 7   KTIPSKSPLPEQQLKKIFKQHDTNGDGQLNKAELKQAFQHLGALIPGWRAHRALHHADTD 66

Query: 108 RDGFIDEKDFEQLIDYAEKRQYTV 132
            DG I EK+ + L++YA K  YT+
Sbjct: 67  GDGCISEKEMKDLVEYAAKFGYTI 90

[Close]

BLAST of CmoCh04G009900 vs. NCBI nr
Match: gi|700207397|gb|KGN62516.1| (hypothetical protein Csa_2G358870 [Cucumis sativus])

HSP 1 Score: 81.3 bits (199), Expect = 1.4e-12
Identity = 35/77 (45.45%), Postives = 57/77 (74.03%), Query Frame = 1


Query: 53  RPKISRQQLRVIFRNHDRDGDGRLSSAELTKAFHYIGALLPSFRACHALLYTDSDRDGFI 112
           R ++SR+ ++ IFR HD DGDGRLS  E+ KAF   G++LP ++A + + + D + DGFI
Sbjct: 7   RLQLSREDIKEIFREHDIDGDGRLSMNEMVKAFGCFGSILPFYKAHYGMAHADENGDGFI 66

Query: 113 DEKDFEQLIDYAEKRQY 130
           +E++ ++L+DYA+K Q+
Sbjct: 67  EEEELDKLVDYAQKFQH 83

[Close]

BLAST of CmoCh04G009900 vs. NCBI nr
Match: gi|449470198|ref|XP_004152805.1| (PREDICTED: probable calcium-binding protein CML10 [Cucumis sativus])

HSP 1 Score: 81.3 bits (199), Expect = 1.4e-12
Identity = 35/77 (45.45%), Postives = 57/77 (74.03%), Query Frame = 1


Query: 53  RPKISRQQLRVIFRNHDRDGDGRLSSAELTKAFHYIGALLPSFRACHALLYTDSDRDGFI 112
           R ++SR+ ++ IFR HD DGDGRLS  E+ KAF   G++LP ++A + + + D + DGFI
Sbjct: 88  RLQLSREDIKEIFREHDIDGDGRLSMNEMVKAFGCFGSILPFYKAHYGMAHADENGDGFI 147

Query: 113 DEKDFEQLIDYAEKRQY 130
           +E++ ++L+DYA+K Q+
Sbjct: 148 EEEELDKLVDYAQKFQH 164

[Close]

BLAST of CmoCh04G009900 vs. NCBI nr
Match: gi|700194510|gb|KGN49687.1| (hypothetical protein Csa_5G067410 [Cucumis sativus])

HSP 1 Score: 78.2 bits (191), Expect = 1.2e-11
Identity = 31/71 (43.66%), Postives = 53/71 (74.65%), Query Frame = 1


Query: 56  ISRQQLRVIFRNHDRDGDGRLSSAELTKAFHYIGALLPSFRACHALLYTDSDRDGFIDEK 115
           +SR++++ IF+ HD + DG L+  ELTKAF ++G++ P ++AC+ ++Y D++ DG I E 
Sbjct: 96  LSRERIKEIFQYHDNNNDGFLNRMELTKAFAFLGSMFPFYKACYGMVYADANEDGLISEA 155

Query: 116 DFEQLIDYAEK 127
           + ++LIDYA K
Sbjct: 156 ELDKLIDYASK 166

The following BLAST results are available for this feature:

BLAST of CmoCh04G009900 vs. Swiss-Prot


Match Name	E-value	Identity	Description

BLAST of CmoCh04G009900 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KZR6_CUCSA	6.4e-28	68.89	Uncharacterized protein OS=Cucumis sativus GN=Csa_4G639730 PE=4 SV=1	[more]
A5AWP8_VITVI	5.9e-13	45.24	Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_023174 PE=4 SV=1	[more]
A0A0A0LRC1_CUCSA	1.0e-12	45.45	Uncharacterized protein OS=Cucumis sativus GN=Csa_2G358870 PE=4 SV=1	[more]
A0A0A0KMQ1_CUCSA	8.5e-12	43.66	Uncharacterized protein OS=Cucumis sativus GN=Csa_5G067410 PE=4 SV=1	[more]
A0A0A0KIY4_CUCSA	1.1e-11	44.44	Uncharacterized protein OS=Cucumis sativus GN=Csa_5G068170 PE=4 SV=1	[more]

BLAST of CmoCh04G009900 vs. TAIR10


Match Name	E-value	Identity	Description
AT1G76650.1	6.2e-06	34.29	calmodulin-like 38	[more]

BLAST of CmoCh04G009900 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|700200027\|gb\|KGN55185.1\|	9.2e-28	68.89	hypothetical protein Csa_4G639730 [Cucumis sativus]	[more]
gi\|147866710\|emb\|CAN78405.1\|	8.4e-13	45.24	hypothetical protein VITISV_023174 [Vitis vinifera]	[more]
gi\|700207397\|gb\|KGN62516.1\|	1.4e-12	45.45	hypothetical protein Csa_2G358870 [Cucumis sativus]	[more]
gi\|449470198\|ref\|XP_004152805.1\|	1.4e-12	45.45	PREDICTED: probable calcium-binding protein CML10 [Cucumis sativus]	[more]
gi\|700194510\|gb\|KGN49687.1\|	1.2e-11	43.66	hypothetical protein Csa_5G067410 [Cucumis sativus]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR002048	EF_hand_dom
IPR011992	EF-hand-dom_pair
IPR018247	EF_Hand_1_Ca_BS

Vocabulary: **Molecular Function**
Term	Definition
GO:0005509	calcium ion binding

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0008150	biological_process
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0005509	calcium ion binding

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmoCh04G009900.1	CmoCh04G009900.1	mRNA

InterPro

Analysis Name: InterPro Annotations of Cucurbita moschata
Date Performed: 2017-05-19


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002048	EF-hand domain	PFAM	PF13405	EF-hand_6	coord: 61..86 score: 1.
IPR002048	EF-hand domain	PROFILE	PS50222	EF_HAND_2	coord: 105..127 score: 7.874coord: 56..91 score: 13
IPR011992	EF-hand domain pair	GENE3D	G3DSA:1.10.238.10		coord: 51..123 score: 1.6
IPR011992	EF-hand domain pair	unknown	SSF47473	EF-hand	coord: 47..122 score: 3.93
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 105..117 score: -coord: 69..81 scor
None	No IPR available	PANTHER	PTHR10891	EF-HAND CALCIUM-BINDING DOMAIN CONTAINING PROTEIN	coord: 57..124 score: 2.3
None	No IPR available	PANTHER	PTHR10891:SF592	CALCIUM-BINDING PROTEIN CML38-RELATED	coord: 57..124 score: 2.3

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
CmoCh04G009900	CmaCh04G009210	Cucurbita maxima (Rimu)	cmacmoB728
CmoCh04G009900	Carg00038	Silver-seed gourd	carcmoB0923

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
CmoCh04G009900	Cucurbita maxima (Rimu)	cmacmoB337
CmoCh04G009900	Wild cucumber (PI 183967)	cmocpiB703
CmoCh04G009900	Cucumber (Chinese Long) v2	cmocuB693
CmoCh04G009900	Melon (DHL92) v3.5.1	cmomeB680
CmoCh04G009900	Watermelon (Charleston Gray)	cmowcgB615
CmoCh04G009900	Cucurbita pepo (Zucchini)	cmocpeB667
CmoCh04G009900	Cucurbita pepo (Zucchini)	cmocpeB673
CmoCh04G009900	Bottle gourd (USVL1VR-Ls)	cmolsiB678
CmoCh04G009900	Bottle gourd (USVL1VR-Ls)	cmolsiB684
CmoCh04G009900	Cucumber (Gy14) v2	cgybcmoB393
CmoCh04G009900	Melon (DHL92) v3.6.1	cmomedB775
CmoCh04G009900	Cucumber (Chinese Long) v3	cmocucB0822
CmoCh04G009900	Watermelon (97103) v2	cmowmbB689
CmoCh04G009900	Cucurbita moschata (Rifu)	cmocmoB285
CmoCh04G009900	Cucumber (Gy14) v1	cgycmoB0118