CmoCh03G010660 (gene) Cucurbita moschata (Rifu)

NameCmoCh03G010660
Typegene
OrganismCucurbita moschata (Cucurbita moschata (Rifu))
DescriptionNodulin MtN21/EamA-like transporter family protein
LocationCmo_Chr03 : 7944503 .. 7946483 (+)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ACCATCACCAAATATGGCTTCCAACAACCCTTACATCGTTGCATTCTTCATCCAAATCACCTACGCCGGTATGAGCCTCATGTCCAAGGCCGCCTTTGCCTCCGGTATGAACACGTATATCTTCCTCTTCTATCGTCAAGCTGCTGGTACTCTTGTTTTGGTCCCGCTTACTCTACTCTTAAAAGGGTAAGTTTCGTTTTCAATGTTTGTTTATTTCGATTTACCAATACGAACGTAGTTCAGTTGATCATTTTGTTGCAAATATATTTCAGGAAGGAGAAGAGGCCGTTGTCTTTGAAGCATTTGTGCCAGATTTTTGTCATTTCATTGATAGGGTGTGTATTAATGTTTATCATTAATGTCCCTAATGTTAGGAATTAGGACTATCCACACCGTGGTATGATATGTGTCTAGTGAGCCTAAGCTCTCTCCGGGAGATGTTGTATTCCCTACTTATAAATCCATAATCATTCTCTTAATTAGTCCTAAAAGGACTCATTCCCATCCTCCCCTCGAACAAAGTACACCATAGAGCCTCTTTTGAGGCCTATGGAGCTCTCGAACAGCCTCCCCTTAATTGAGGCTCGACTCCTTTCTCTGGAGACCTCGAACAAAATACCTCTTTTGTTTGACACTTGAGTCACTCTTGACTACACTTGCGAGGGTCCCAACTTATTTGTTCGACATTCGATGATTCTATTGACACGACTAAGTTAAAACATGACTCTAATACCATATTAAGAATTACGACTCTCTAATGTAGGACTCCCTTCCAACGATCCTCAACACCTAATGTATATCAACTTTTTATTTTATTGAATATAGTCTCAATTATGAATTGGGTTTAGGATCACTCTAGCATTGGATGCATATGGAGTAGCGATTAACTATACGTCGGCAACATTGGGCGCCGCAGCTTTCAACTGCGTTCCTGTCACCACATTCTTCTTCGCACTCATACTAAGAATGGAGAAGGTGAAGCTAAATAAAGCAGCCGGGATAGCAAAGGTGGTGGGCATAATGATATGCATGGCGGGCGCCGCCATTCTAGCCTTCTACAAAGGCCCATATTTGAGCCCAATTTTGAGCCACCACCAACTATTCCACCACGTCCAAACCCAAACCCAAACCCACCACCCTCCCACCTCCCAAAACACATGGATGATCGGCTGCTTCTTCCTCCTCGTCACCAGCGTCTCTTGGGGGATCTGGTTCGTTCTCCAGGCTCGATTCTTGAAGGAGTACCCGCACCCGATGGAGTTCATGTGCGCGCAGACCGTCATGAGCACGGCTCAGACCTTCTTCGTAGCCATCGCCATGGAAAGAGACCCTTTGGAGTGGAAATTGGGCTGGAATGTCCGACTCTACGCTGTGCTCTATTGTGTAAGCTTAATCAAATCACGAGTTGTTGGGCTTACCCAAAGATGTAATCTTTACTTATAAATCTATGATCATTTTCTAAATTAGCTTAACATGTGGTGGTGCAGGGAGTGCTAGTCATTGGCGTGGCAAATAATGCCCAATGTTGGGTCATCAGAGAGAAGGGTCCAGTTTTTCAAGCCATGACGACGCCGTTGAACTTAATGGCTACAATAATTGGTTCCCTATTGCTACTTTCCGAAGGAATTTACGTGGGAAGGTAATAAATTACGGTAATTTAAGCTGTAAATTTTTGTGTTAAAAGTTACTGAATTTAGTGTTGTTGTTGTTGGGATGACAGTATAATAGGAGCATCGTTGCTGGTAATGAGTCTTTACAGTGTTTTGTGGGGTAAAAGCAAGGAGCTTGTCACTGCTCCAAACGACCAACAACCGTCTCCACCAATAAAACAGGTTGAAGAGTCGACATGTCGTTCTGAAGTCAACTCCACGTTAGTCTAAGCAAAAGCTGTGATGAACTTGTTCGTGATATTTAATTTCTTCTCTAAAATTGTATTTTGGTTACTAAATGCAATACAATTCTCCTATCAATTTCAATATTA

mRNA sequence

ACCATCACCAAATATGGCTTCCAACAACCCTTACATCGTTGCATTCTTCATCCAAATCACCTACGCCGGTATGAGCCTCATGTCCAAGGCCGCCTTTGCCTCCGGTATGAACACGTATATCTTCCTCTTCTATCGTCAAGCTGCTGGTACTCTTGTTTTGGTCCCGCTTACTCTACTCTTAAAAGGGAAGGAGAAGAGGCCGTTGTCTTTGAAGCATTTGTGCCAGATTTTTGTCATTTCATTGATAGGGATCACTCTAGCATTGGATGCATATGGAGTAGCGATTAACTATACGTCGGCAACATTGGGCGCCGCAGCTTTCAACTGCGTTCCTGTCACCACATTCTTCTTCGCACTCATACTAAGAATGGAGAAGGTGAAGCTAAATAAAGCAGCCGGGATAGCAAAGGTGGTGGGCATAATGATATGCATGGCGGGCGCCGCCATTCTAGCCTTCTACAAAGGCCCATATTTGAGCCCAATTTTGAGCCACCACCAACTATTCCACCACGTCCAAACCCAAACCCAAACCCACCACCCTCCCACCTCCCAAAACACATGGATGATCGGCTGCTTCTTCCTCCTCGTCACCAGCGTCTCTTGGGGGATCTGGTTCGTTCTCCAGGCTCGATTCTTGAAGGAGTACCCGCACCCGATGGAGTTCATGTGCGCGCAGACCGTCATGAGCACGGCTCAGACCTTCTTCGTAGCCATCGCCATGGAAAGAGACCCTTTGGAGTGGAAATTGGGCTGGAATGTCCGACTCTACGCTGTGCTCTATTGTGGAGTGCTAGTCATTGGCGTGGCAAATAATGCCCAATGTTGGGTCATCAGAGAGAAGGGTCCAGTTTTTCAAGCCATGACGACGCCGTTGAACTTAATGGCTACAATAATTGGTTCCCTATTGCTACTTTCCGAAGGAATTTACGTGGGAAGTATAATAGGAGCATCGTTGCTGGTAATGAGTCTTTACAGTGTTTTGTGGGGTAAAAGCAAGGAGCTTGTCACTGCTCCAAACGACCAACAACCGTCTCCACCAATAAAACAGGTTGAAGAGTCGACATGTCGTTCTGAAGTCAACTCCACGTTAGTCTAAGCAAAAGCTGTGATGAACTTGTTCGTGATATTTAATTTCTTCTCTAAAATTGTATTTTGGTTACTAAATGCAATACAATTCTCCTATCAATTTCAATATTA

Coding sequence (CDS)

ATGGCTTCCAACAACCCTTACATCGTTGCATTCTTCATCCAAATCACCTACGCCGGTATGAGCCTCATGTCCAAGGCCGCCTTTGCCTCCGGTATGAACACGTATATCTTCCTCTTCTATCGTCAAGCTGCTGGTACTCTTGTTTTGGTCCCGCTTACTCTACTCTTAAAAGGGAAGGAGAAGAGGCCGTTGTCTTTGAAGCATTTGTGCCAGATTTTTGTCATTTCATTGATAGGGATCACTCTAGCATTGGATGCATATGGAGTAGCGATTAACTATACGTCGGCAACATTGGGCGCCGCAGCTTTCAACTGCGTTCCTGTCACCACATTCTTCTTCGCACTCATACTAAGAATGGAGAAGGTGAAGCTAAATAAAGCAGCCGGGATAGCAAAGGTGGTGGGCATAATGATATGCATGGCGGGCGCCGCCATTCTAGCCTTCTACAAAGGCCCATATTTGAGCCCAATTTTGAGCCACCACCAACTATTCCACCACGTCCAAACCCAAACCCAAACCCACCACCCTCCCACCTCCCAAAACACATGGATGATCGGCTGCTTCTTCCTCCTCGTCACCAGCGTCTCTTGGGGGATCTGGTTCGTTCTCCAGGCTCGATTCTTGAAGGAGTACCCGCACCCGATGGAGTTCATGTGCGCGCAGACCGTCATGAGCACGGCTCAGACCTTCTTCGTAGCCATCGCCATGGAAAGAGACCCTTTGGAGTGGAAATTGGGCTGGAATGTCCGACTCTACGCTGTGCTCTATTGTGGAGTGCTAGTCATTGGCGTGGCAAATAATGCCCAATGTTGGGTCATCAGAGAGAAGGGTCCAGTTTTTCAAGCCATGACGACGCCGTTGAACTTAATGGCTACAATAATTGGTTCCCTATTGCTACTTTCCGAAGGAATTTACGTGGGAAGTATAATAGGAGCATCGTTGCTGGTAATGAGTCTTTACAGTGTTTTGTGGGGTAAAAGCAAGGAGCTTGTCACTGCTCCAAACGACCAACAACCGTCTCCACCAATAAAACAGGTTGAAGAGTCGACATGTCGTTCTGAAGTCAACTCCACGTTAGTCTAA
BLAST of CmoCh03G010660 vs. Swiss-Prot
Match: WTR45_ARATH (WAT1-related protein At5g64700 OS=Arabidopsis thaliana GN=At5g64700 PE=2 SV=1)

HSP 1 Score: 302.0 bits (772), Expect = 8.8e-81
Identity = 158/340 (46.47%), Postives = 219/340 (64.41%), Query Frame = 1

Query: 1   MASNNPYIVAFFIQITYAGMSLMSKAAFASGMNTYIFLFYRQAAGTLVLVPLTLLLKGKE 60
           M S  PY++   IQ+ Y  M L+SKA F  GMNT++F+FYRQA  T+ L PL    + K 
Sbjct: 3   MESKKPYLMVTIIQVIYTIMFLISKAVFNGGMNTFVFVFYRQAFATIFLAPLAFFFERKS 62

Query: 61  KRPLSLKHLCQIFVISLIGITLALDAYGVAINYTSATLGAAAFNCVPVTTFFFALILRME 120
             PLS     +IF++SL G+TL+LD  G+A++YTSATL AA    +P  TFF AL+  ME
Sbjct: 63  APPLSFVTFIKIFMLSLFGVTLSLDLNGIALSYTSATLAAATTASLPAITFFLALLFGME 122

Query: 121 KVKLNKAAGIAKVVGIMICMAGAAILAFYKGPYLSPILSHHQLFHHVQTQTQTHHP---P 180
           ++K+    G AK+VGI +CM G  ILA YKGP L   L  H  F+H Q     ++P    
Sbjct: 123 RLKVKSIQGTAKLVGITVCMGGVIILAIYKGPLLKLPLCPH--FYHGQEHPHRNNPGHVS 182

Query: 181 TSQNTWMIGCFFLLVTSVSWGIWFVLQARFLKEYPHPMEFMCAQTVMSTAQTFFVAIAME 240
               +W+ GC  ++ +++ WG+W VLQ R LK YP  + F     ++S+ Q+F +AIA+E
Sbjct: 183 GGSTSWLKGCVLMITSNILWGLWLVLQGRVLKVYPSKLYFTTLHCLLSSIQSFVIAIALE 242

Query: 241 RDPLEWKLGWNVRLYAVLYCGVLVIGVANNAQCWVIREKGPVFQAMTTPLNLMATIIGSL 300
           RD   WKLGWN+RL AV+YCG +V GVA   Q WVI ++GPVF +M TPL+L+ T++ S 
Sbjct: 243 RDISAWKLGWNLRLVAVIYCGFIVTGVAYYLQSWVIEKRGPVFLSMFTPLSLLFTLLSSA 302

Query: 301 LLLSEGIYVGSIIGASLLVMSLYSVLWGKSKELVTAPNDQ 338
           +LL E I +GSI+G  LL++ LY VLWGKS+E   + +D+
Sbjct: 303 ILLCEIISLGSIVGGLLLIIGLYCVLWGKSREEKNSGDDK 340

BLAST of CmoCh03G010660 vs. Swiss-Prot
Match: WTR7_ARATH (WAT1-related protein At1g43650 OS=Arabidopsis thaliana GN=At1g43650 PE=2 SV=1)

HSP 1 Score: 236.5 bits (602), Expect = 4.5e-61
Identity = 133/329 (40.43%), Postives = 188/329 (57.14%), Query Frame = 1

Query: 1   MASNNPYIVAFFIQITYAGMSLMSKAAFASGMNTYIFLFYRQAAGTLVLVPLTLLLKGKE 60
           M  +   +   F+QI YAGM L+SK A + G N ++F+FYRQA   L L P    L+  +
Sbjct: 2   MMEHKANMAMVFVQIVYAGMPLLSKVAISQGTNPFVFVFYRQAFAALALSPFAFFLESSK 61

Query: 61  KRPLSLKHLCQIFVISLIGITLALDAYGVAINYTSATLGAAAFNCVPVTTFFFALILRME 120
             PLS   L +IF ISL G+TL+L+ Y VAI  T+AT  AA  N +P  TF  AL+ R+E
Sbjct: 62  SSPLSFILLLKIFFISLCGLTLSLNLYYVAIENTTATFAAATTNAIPSITFVLALLFRLE 121

Query: 121 KVKLNKAAGIAKVVGIMICMAGAAILAFYKGPYLSPILSHHQLFHHVQTQTQTHHPPTSQ 180
            V L K+ G+AKV G M+ M GA + AF KGP          L +H  + T  +    S 
Sbjct: 122 TVTLKKSHGVAKVTGSMVGMLGALVFAFVKGP---------SLINHYNSSTIPNGTVPST 181

Query: 181 NTWMIGCFFLLVTSVSWGIWFVLQARFLKEYPHPMEFMCAQTVMSTAQTFFVAIAMERDP 240
              + G   +L  +  W +W ++Q++ +KEYP  +  +  Q + S  Q+   A+A+ R+P
Sbjct: 182 KNSVKGSITMLAANTCWCLWIIMQSKVMKEYPAKLRLVALQCLFSCIQSAVWAVAVNRNP 241

Query: 241 LEWKLGWNVRLYAVLYCGVLVIGVANNAQCWVIREKGPVFQAMTTPLNLMATIIGSLLLL 300
             WK+ + + L ++ YCG++V G+    Q W I +KGPVF A+ TPL L+ T I S  L 
Sbjct: 242 SVWKIEFGLPLLSMAYCGIMVTGLTYWLQVWAIEKKGPVFTALYTPLALILTCIVSSFLF 301

Query: 301 SEGIYVGSIIGASLLVMSLYSVLWGKSKE 330
            E  Y+GS+ GA LLV  LY  LWGK+KE
Sbjct: 302 KETFYLGSVGGAVLLVCGLYLGLWGKTKE 321

BLAST of CmoCh03G010660 vs. Swiss-Prot
Match: WAT1_ARATH (Protein WALLS ARE THIN 1 OS=Arabidopsis thaliana GN=WAT1 PE=1 SV=1)

HSP 1 Score: 195.3 bits (495), Expect = 1.2e-48
Identity = 114/326 (34.97%), Postives = 172/326 (52.76%), Query Frame = 1

Query: 7   YIVAFFIQITYAGMSLMSKAAFASGMNTYIFLFYRQAAGTLVLVPLTLLLKGKEKRPLSL 66
           +I    +Q  YAG  ++S+AA   G++  +F  YR     L+L+P    L+ KE+  ++L
Sbjct: 21  HIAMLTLQFGYAGFHVVSRAALNMGISKLVFPVYRNIIALLLLLPFAYFLEKKERPAITL 80

Query: 67  KHLCQIFVISLIGITLALDAYGVAINYTSATLGAAAFNCVPVTTFFFALILRMEKVKLNK 126
             L Q F ++LIGIT     Y + ++ TS T  ++  N VP  TF  A +LR+EKV++N+
Sbjct: 81  NFLIQFFFLALIGITANQGFYLLGLDNTSPTFASSMQNSVPAITFLMAALLRIEKVRINR 140

Query: 127 AAGIAKVVGIMICMAGAAILAFYKGPYLSPILSHHQLFHHVQTQTQTHHPP---TSQNTW 186
             GI+K++G  +C+AGA+++  YKGP +    SH  L  H+ T       P    +   W
Sbjct: 141 RDGISKILGTALCVAGASVITLYKGPTIYTPASH--LHAHLLTTNSAVLAPLGNAAPKNW 200

Query: 187 MIGCFFLLVTSVSWGIWFVLQARFLKEYPHPMEFMCAQTVMSTAQTFFVAIAMERDPLEW 246
            +GC +L+   +SW  W V QA  LK YP  +            Q   +A   ERD   W
Sbjct: 201 TLGCIYLIGHCLSWSGWLVFQAPVLKSYPARLSVTSYTCFFGIIQFLIIAAFCERDSQAW 260

Query: 247 KLGWNVRLYAVLYCGVLVIGVANNAQCWVIREKGPVFQAMTTPLNLMATIIGSLLLLSEG 306
                  L+ +LY G++  G+A   Q W I   GPVF A+  P+  +   I + + L E 
Sbjct: 261 VFHSGWELFTILYAGIVASGIAFAVQIWCIDRGGPVFVAVYQPVQTLVVAIMASIALGEE 320

Query: 307 IYVGSIIGASLLVMSLYSVLWGKSKE 330
            Y+G IIGA L++  LY VL+GKS+E
Sbjct: 321 FYLGGIIGAVLIIAGLYFVLYGKSEE 344

BLAST of CmoCh03G010660 vs. Swiss-Prot
Match: WTR38_ARATH (WAT1-related protein At5g07050 OS=Arabidopsis thaliana GN=At5g07050 PE=2 SV=1)

HSP 1 Score: 189.5 bits (480), Expect = 6.4e-47
Identity = 104/334 (31.14%), Postives = 179/334 (53.59%), Query Frame = 1

Query: 1   MASNNPYIVAFFIQITYAGMSLMSKAAFASGMNTYIFLFYRQAAGTLVLVPLTLLLKGKE 60
           + S+ PY     +Q  YAGM++++K +  +GM+ Y+ + YR A  T V+ P     + K 
Sbjct: 13  LTSSKPYFAMISLQFGYAGMNIITKISLNTGMSHYVLVVYRHAIATAVIAPFAFFFERKA 72

Query: 61  KRPLSLKHLCQIFVISLIGITLALDAYGVAINYTSATLGAAAFNCVPVTTFFFALILRME 120
           +  ++     Q+F++ L+G  +  + Y + + YTS T   A  N +P  TF  A++ RME
Sbjct: 73  QPKITFSIFMQLFILGLLGPVIDQNFYYMGLKYTSPTFSCAMSNMLPAMTFILAVLFRME 132

Query: 121 KVKLNKAAGIAKVVGIMICMAGAAILAFYKGPYLSPILSHHQLFHHVQ----TQTQTHHP 180
            + L K    AK+ G ++ +AGA ++  YKGP +    + +    H+Q      T +   
Sbjct: 133 MLDLKKLWCQAKIAGTVVTVAGAMLMTIYKGPIVELFWTKYM---HIQDSSHANTTSSKN 192

Query: 181 PTSQNTWMIGCFFLLVTSVSWGIWFVLQARFLKEY-PHPMEFMCAQTVMSTAQTFFVAIA 240
            +S   ++ G   L+  +++W   FVLQA+ LK Y  H +        + T Q   V   
Sbjct: 193 SSSDKEFLKGSILLIFATLAWASLFVLQAKILKTYAKHQLSLTTLICFIGTLQAVAVTFV 252

Query: 241 MERDPLEWKLGWNVRLYAVLYCGVLVIGVANNAQCWVIREKGPVFQAMTTPLNLMATIIG 300
           ME +P  W++GW++ L A  Y G++   ++   Q  V++++GPVF    +PL ++   + 
Sbjct: 253 MEHNPSAWRIGWDMNLLAAAYSGIVASSISYYVQGIVMKKRGPVFATAFSPLMMVIVAVM 312

Query: 301 SLLLLSEGIYVGSIIGASLLVMSLYSVLWGKSKE 330
              +L+E I++G +IGA L+V+ LY+VLWGK KE
Sbjct: 313 GSFVLAEKIFLGGVIGAVLIVIGLYAVLWGKQKE 343

BLAST of CmoCh03G010660 vs. Swiss-Prot
Match: WTR8_ARATH (WAT1-related protein At1g44800 OS=Arabidopsis thaliana GN=At1g44800 PE=1 SV=1)

HSP 1 Score: 187.6 bits (475), Expect = 2.4e-46
Identity = 110/338 (32.54%), Postives = 184/338 (54.44%), Query Frame = 1

Query: 1   MASNNPYIVAFFIQITYAGMSLMSKAAFASGMNTYIFLFYRQAAGTLVLVPLTLLLKGKE 60
           M    P +    +Q  YAGM +++  +F  GM+ ++   YR    T+V+ P  L+ + K 
Sbjct: 6   MEKIKPILAIISLQFGYAGMYIITMVSFKHGMDHWVLATYRHVVATVVMAPFALMFERKI 65

Query: 61  KRPLSLKHLCQIFVISLIGITLALDAYGVAINYTSATLGAAAFNCVPVTTFFFALILRME 120
           +  ++L    ++  + ++   +  + Y + +  TSA+  +A  N +P  TF  ALI R+E
Sbjct: 66  RPKMTLAIFWRLLALGILEPLMDQNLYYIGLKNTSASYTSAFTNALPAVTFILALIFRLE 125

Query: 121 KVKLNKAAGIAKVVGIMICMAGAAILAFYKGPYLSPILSHHQLFHHVQTQTQTHHPPTSQ 180
            V   K   +AKVVG +I + GA I+  YKGP +  + + H  FH   + T     PT Q
Sbjct: 126 TVNFRKVHSVAKVVGTVITVGGAMIMTLYKGPAIEIVKAAHNSFHGGSSST-----PTGQ 185

Query: 181 NTWMIGCFFLLVTSVSWGIWFVLQARFLKEYPHPMEFMCAQTVMSTAQTFFVAIAMERDP 240
           + W++G   ++ +  +W  +F+LQ+  LK YP  +  +     + T      ++ M RDP
Sbjct: 186 H-WVLGTIAIMGSISTWAAFFILQSYTLKVYPAELSLVTLICGIGTILNAIASLIMVRDP 245

Query: 241 LEWKLGWNVRLYAVLYCGVLVIGVANNAQCWVIREKGPVFQAMTTPLNLMATIIGSLLLL 300
             WK+G +    A +Y GV+  G+A   Q  VI+++GPVF    +P+ ++ T     L+L
Sbjct: 246 SAWKIGMDSGTLAAVYSGVVCSGIAYYIQSIVIKQRGPVFTTSFSPMCMIITAFLGALVL 305

Query: 301 SEGIYVGSIIGASLLVMSLYSVLWGKSKELVTAPNDQQ 339
           +E I++GSIIGA  +V+ LYSV+WGKSK+ V  P D++
Sbjct: 306 AEKIHLGSIIGAVFIVLGLYSVVWGKSKDEVN-PLDEK 336

BLAST of CmoCh03G010660 vs. TrEMBL
Match: A0A0A0KFA3_CUCSA (WAT1-related protein OS=Cucumis sativus GN=Csa_6G485160 PE=3 SV=1)

HSP 1 Score: 568.2 bits (1463), Expect = 7.3e-159
Identity = 288/360 (80.00%), Postives = 318/360 (88.33%), Query Frame = 1

Query: 1   MASNNPYIVAFFIQITYAGMSLMSKAAFASGMNTYIFLFYRQAAGTLVLVPLTLLLKGKE 60
           M SN PYIV FFIQIT+AGMSLMSKAAFA+GMNTYIFLFYRQAAG+++LVPLTL+LKGKE
Sbjct: 1   MGSNKPYIVVFFIQITFAGMSLMSKAAFAAGMNTYIFLFYRQAAGSIILVPLTLILKGKE 60

Query: 61  KRPLSLKHLCQIFVISLIGITLALDAYGVAINYTSATLGAAAFNCVPVTTFFFALILRME 120
           KRPLSLKHLCQIFVISLIGITLALDAYGVAINYTSAT+GAAAFNCVPVTTFFFA++LRME
Sbjct: 61  KRPLSLKHLCQIFVISLIGITLALDAYGVAINYTSATIGAAAFNCVPVTTFFFAVLLRME 120

Query: 121 KVKLNKAAGIAKVVGIMICMAGAAILAFYKGPYLSPILSHHQLFHHVQTQTQTHHPPTSQ 180
           KV L KAAGIAKVVGIMIC+ GA+ILA YKGPYL P+ +H QLFHH+ +Q   HH   S 
Sbjct: 121 KVNLKKAAGIAKVVGIMICITGASILALYKGPYLKPLFTH-QLFHHIHSQP--HHSLNSH 180

Query: 181 NTWMIGCFFLLVTSVSWGIWFVLQARFLKEYPHPMEFMCAQTVMSTAQTFFVAIAMERDP 240
             WMIGCFFLLVTS+SWGIWFVLQARFLK YPHPMEFMC QT+MS  Q+F VAI MERDP
Sbjct: 181 TKWMIGCFFLLVTSISWGIWFVLQARFLKGYPHPMEFMCMQTLMSMVQSFIVAIVMERDP 240

Query: 241 LEWKLGWNVRLYAVLYCGVLVIGVANNAQCWVIREKGPVFQAMTTPLNLMATIIGSLLLL 300
            EWKLG+N+RL+AVLYCG+LVIGVANNAQCWVIREKGPVFQAMTTPLNL+ATIIGS  L 
Sbjct: 241 FEWKLGFNIRLFAVLYCGILVIGVANNAQCWVIREKGPVFQAMTTPLNLIATIIGSQFLF 300

Query: 301 SEGIYVGSIIGASLLVMSLYSVLWGKSKELVTAPNDQQPSPPIKQVEESTCRSEVNSTLV 360
            +G Y+GSIIGA LLV SLY VLWGK+KELVT  ND Q SPP K+ +ESTC +EVNS+LV
Sbjct: 301 PDGTYLGSIIGAFLLVTSLYCVLWGKNKELVTPSNDGQLSPPQKETKESTCPTEVNSSLV 357

BLAST of CmoCh03G010660 vs. TrEMBL
Match: A0A0A0KJ33_CUCSA (WAT1-related protein OS=Cucumis sativus GN=Csa_6G485670 PE=3 SV=1)

HSP 1 Score: 516.5 bits (1329), Expect = 2.5e-143
Identity = 264/365 (72.33%), Postives = 308/365 (84.38%), Query Frame = 1

Query: 1   MASNNPYIVAFFIQITYAGMSLMSKAAFASGMNTYIFLFYRQAAGTLVLVPLTLLLKGKE 60
           M S  PY+VA FIQIT+AGMSL+SKAAFA+GMNTYIFLFYRQAAG+L+L+PLTLLLKGKE
Sbjct: 1   MDSKKPYLVAIFIQITFAGMSLLSKAAFATGMNTYIFLFYRQAAGSLILIPLTLLLKGKE 60

Query: 61  KRPLSLKHLCQIFVISLIGITLALDAYGVAINYTSATLGAAAFNCVPVTTFFFALILRME 120
           KRPLS K LCQ F ISLIGITLA++AYGVA++YTSATLGAAAFNC+PV+TF FA++ RME
Sbjct: 61  KRPLSFKQLCQCFFISLIGITLAMNAYGVAVDYTSATLGAAAFNCLPVSTFIFAVLFRME 120

Query: 121 KVKLNKAAGIAKVVGIMICMAGAAILAFYKGPYLSPILSHHQLFHHVQTQTQTHHPPTSQ 180
           +V L KAAGIAKV G+MIC+ GAAILAFYKGPYL PI+SH  +FH  +++T      TSQ
Sbjct: 121 RVNLKKAAGIAKVGGMMICVGGAAILAFYKGPYLKPIISH-PIFHIEESETDI--TTTSQ 180

Query: 181 NTWMIGCFFLLVTSVSWGIWFVLQARFLKEYPHPMEFMCAQTVMSTAQTFFVAIAMERDP 240
            +W++GCFFLLV +V WGIWFV QA+FLK YPHP+EFMCAQTVMS  Q F VAI +ERDP
Sbjct: 181 KSWLLGCFFLLVATVGWGIWFVFQAKFLKGYPHPVEFMCAQTVMSVVQCFVVAIIVERDP 240

Query: 241 LEWKLGWNVRLYAVLYCGVLVIGVANNAQCWVIREKGPVFQAMTTPLNLMATIIGSLLLL 300
            EWKLGWNVRLYAVLYCG+LVIG+ANNAQCWVI+EKGPVFQAM  PLNL+ATIIGS L L
Sbjct: 241 SEWKLGWNVRLYAVLYCGILVIGIANNAQCWVIKEKGPVFQAMMMPLNLVATIIGSQLFL 300

Query: 301 SEGIYVGSIIGASLLVMSLYSVLWGKSKELVTAPNDQQ-----PSPPIKQVEESTCRSEV 360
           +EGIY+GS+IGA LLV SLYSVLWGK+KELV  P +Q+      S P K+ EE   RS+V
Sbjct: 301 AEGIYLGSVIGAILLVTSLYSVLWGKNKELVVTPTNQERPSSPDSLPQKESEEPANRSQV 360

BLAST of CmoCh03G010660 vs. TrEMBL
Match: A0A0A0KKW0_CUCSA (WAT1-related protein OS=Cucumis sativus GN=Csa_6G485170 PE=3 SV=1)

HSP 1 Score: 473.0 bits (1216), Expect = 3.2e-130
Identity = 245/362 (67.68%), Postives = 288/362 (79.56%), Query Frame = 1

Query: 1   MASNNPYIVAFFIQITYAGMSLMSKAAFASGMNTYIFLFYRQAAGTLVLVPLTLLLKGKE 60
           M S   YIVA F QI YAGMSL SK AFA+GMNTY+FLFYRQAAG+L+L+PLTLLLKGKE
Sbjct: 1   MGSKKFYIVAIFTQIIYAGMSLSSKVAFATGMNTYVFLFYRQAAGSLILIPLTLLLKGKE 60

Query: 61  KRPLSLKHLCQIFVISLIGITLALDAYGVAINYTSATLGAAAFNCVPVTTFFFALILRME 120
           KRPLSLK LC  F ISLIGITL ++AY V I+YTS T GAAA NC+P++TF FA+  RME
Sbjct: 61  KRPLSLKQLCHAFFISLIGITLTMNAYDVGIDYTSPTFGAAAINCIPISTFIFAVTFRME 120

Query: 121 KVKLNKAAGIAKVVGIMICMAGAAILAFYKGPYLSPILSHHQLFHHVQTQTQTHHPPTSQ 180
           KV L KA+GIAKV G+MIC+ GAAILAFYKGPYL PI+SH  +FH  +++T      TSQ
Sbjct: 121 KVNLKKASGIAKVGGMMICVGGAAILAFYKGPYLKPIISH-PIFHFEESETDI--TTTSQ 180

Query: 181 NTWMIGCFFLLVTSVSWGIWFVLQARFLKEYPHPMEFMCAQTVMSTAQTFFVAIAMERDP 240
            +WM+GCF LLV ++ WGIW+V QA FLK+YPHP+E MC QTVMS  Q F VAI +ERD 
Sbjct: 181 KSWMLGCFILLVATMGWGIWYVFQAMFLKDYPHPVELMCTQTVMSVVQCFVVAIIVERDH 240

Query: 241 LEWKLGWNVRLYAVLYCGVLVIGVANNAQCWVIREKGPVFQAMTTPLNLMATIIGSLLLL 300
            EWKL WNVRLYA+LYCG+LVIG+ANNAQCWVI+E GPVF +M  P+NL+ATIIGS L L
Sbjct: 241 SEWKLDWNVRLYAILYCGILVIGIANNAQCWVIKETGPVFPSMMMPINLVATIIGSQLFL 300

Query: 301 SEGIYVGSIIGASLLVMSLYSVLWGKSKELVTAP-NDQQP-SP---PIKQVEESTCRSEV 358
           +EGIY+GS+IGA LLV+SLYSVLWGKSKELV  P N  QP SP   P K+ EE   RS+V
Sbjct: 301 AEGIYLGSVIGAILLVISLYSVLWGKSKELVDTPTNQDQPFSPDFLPQKESEEQDSRSQV 359

BLAST of CmoCh03G010660 vs. TrEMBL
Match: A0A0A0KHV4_CUCSA (WAT1-related protein OS=Cucumis sativus GN=Csa_6G485150 PE=3 SV=1)

HSP 1 Score: 384.0 bits (985), Expect = 2.0e-103
Identity = 198/317 (62.46%), Postives = 240/317 (75.71%), Query Frame = 1

Query: 20  MSLMSKAAFASGMNTYIFLFYRQAAGTLVLVPLTLLLKGKEKRPLSLKHLCQIFVISLIG 79
           MSL+SKAAFASGMNT++                      KE R LSLK   +IF+ISLIG
Sbjct: 1   MSLLSKAAFASGMNTFV----------------------KESRSLSLKDFLKIFMISLIG 60

Query: 80  ITLALDAYGVAINYTSATLGAAAFNCVPVTTFFFALILRMEKVKLNKAAGIAKVVGIMIC 139
           +T+  +AYGVA++YTSA LGAAAFNC+PVTTF FA++LRMEKV L K AGIAK +GI+IC
Sbjct: 61  MTIGFNAYGVAVDYTSANLGAAAFNCLPVTTFLFAVLLRMEKVNLRKVAGIAKALGILIC 120

Query: 140 MAGAAILAFYKGPYLSPILSHHQLFHHVQTQTQTHHPPTSQNTWMIGCFFLLVTSVSWGI 199
           + G   LAFYKGPYL P+++HH L  H  +    H   +S  TW+IGCF L ++S+SWG+
Sbjct: 121 IGGVITLAFYKGPYLKPLINHHLLKFHKSSHNIPH--SSSSKTWIIGCFLLFISSISWGL 180

Query: 200 WFVLQARFLKEYPHPMEFMCAQTVMSTAQTFFVAIAMERDPLEWKLGWNVRLYAVLYCGV 259
           WFVLQA FLK YP P+EF+  QT++S AQ+F +AIAMERDP EWKLGWN+RL AV+YCGV
Sbjct: 181 WFVLQAYFLKTYPSPLEFISYQTLLSAAQSFVIAIAMERDPSEWKLGWNIRLLAVVYCGV 240

Query: 260 LVIGVANNAQCWVIREKGPVFQAMTTPLNLMATIIGSLLLLSEGIYVGSIIGASLLVMSL 319
           LV  V+N  QCWVI+EKGPVFQAMTTPLN++ATIIGS LLL EGI +GS+IGA LLV+SL
Sbjct: 241 LVTVVSNFLQCWVIKEKGPVFQAMTTPLNVIATIIGSELLLGEGINLGSLIGAILLVISL 293

Query: 320 YSVLWGKSKELVTAPND 337
           YSVLWGK+KEL  A  D
Sbjct: 301 YSVLWGKNKELDVADPD 293

BLAST of CmoCh03G010660 vs. TrEMBL
Match: M5X3D1_PRUPE (WAT1-related protein OS=Prunus persica GN=PRUPE_ppa015904mg PE=3 SV=1)

HSP 1 Score: 381.7 bits (979), Expect = 9.7e-103
Identity = 193/360 (53.61%), Postives = 255/360 (70.83%), Query Frame = 1

Query: 1   MASNNPYIVAFFIQITYAGMSLMSKAAFASGMNTYIFLFYRQAAGTLVLVPLTLLLKGKE 60
           M S  PY+   FIQI YAGM L+SKAAF  GMN+Y+F+FYRQ AG+L + PL ++ + K 
Sbjct: 1   MGSTKPYLAVIFIQIIYAGMILLSKAAFNGGMNSYVFVFYRQMAGSLFMSPLAMIFERKS 60

Query: 61  KRPLSLKHLCQIFVISLIGITLALDAYGVAINYTSATLGAAAFNCVPVTTFFFALILRME 120
             PLS+   C+IF++S +GITLA++AY +A+ YTSATLGAA+ NC+PV TFFFA++LRME
Sbjct: 61  ATPLSVVTFCKIFMLSFLGITLAINAYCIALTYTSATLGAASINCLPVATFFFAVLLRME 120

Query: 121 KVKLNKAAGIAKVVGIMICMAGAAILAFYKGPYLSPILSHHQLFHHVQTQTQTH-HPPTS 180
           KVK+  AAGIAKV G+++ M G A+L+FYKGP L P L HH L  H   Q Q   H  +S
Sbjct: 121 KVKIKTAAGIAKVAGLVVSMIGVAVLSFYKGPQLKPFLHHHLLQSHNSHQDQQQAHASSS 180

Query: 181 QNTWMIGCFFLLVTSVSWGIWFVLQARFLKEYPHPMEFMCAQTVMSTAQTFFVAIAMERD 240
            + WMIGCF LLV+ + W +W VLQA+ L +YP  + F   Q + S AQ+F VAIA+ERD
Sbjct: 181 GHRWMIGCFLLLVSIICWSLWLVLQAQLLVKYPAKLRFTSLQCLSSAAQSFVVAIALERD 240

Query: 241 PLEWKLGWNVRLYAVLYCGVLVIGVANNAQCWVIREKGPVFQAMTTPLNLMATIIGSLLL 300
           P +W LGWN+ L A+ YCG++V GV+   Q +VI +KGPVFQAM+ PLNL+ T+IGS+LL
Sbjct: 241 PSQWNLGWNINLLAIAYCGIMVTGVSYYLQAYVIEKKGPVFQAMSQPLNLIITVIGSVLL 300

Query: 301 LSEGIYVGSIIGASLLVMSLYSVLWGKSKELVTAPNDQQPSPPIKQVEESTCRSEVNSTL 360
           L E I +GS++G  L+  SLYSVLWGKSKEL +  N    S P++  +E     E  +T+
Sbjct: 301 LGEAISLGSVLGGILMAASLYSVLWGKSKELKSMENRTCLSVPVQPEKERAQLKEAEATI 360

BLAST of CmoCh03G010660 vs. TAIR10
Match: AT5G64700.1 (AT5G64700.1 nodulin MtN21 /EamA-like transporter family protein)

HSP 1 Score: 302.0 bits (772), Expect = 5.0e-82
Identity = 158/340 (46.47%), Postives = 219/340 (64.41%), Query Frame = 1

Query: 1   MASNNPYIVAFFIQITYAGMSLMSKAAFASGMNTYIFLFYRQAAGTLVLVPLTLLLKGKE 60
           M S  PY++   IQ+ Y  M L+SKA F  GMNT++F+FYRQA  T+ L PL    + K 
Sbjct: 3   MESKKPYLMVTIIQVIYTIMFLISKAVFNGGMNTFVFVFYRQAFATIFLAPLAFFFERKS 62

Query: 61  KRPLSLKHLCQIFVISLIGITLALDAYGVAINYTSATLGAAAFNCVPVTTFFFALILRME 120
             PLS     +IF++SL G+TL+LD  G+A++YTSATL AA    +P  TFF AL+  ME
Sbjct: 63  APPLSFVTFIKIFMLSLFGVTLSLDLNGIALSYTSATLAAATTASLPAITFFLALLFGME 122

Query: 121 KVKLNKAAGIAKVVGIMICMAGAAILAFYKGPYLSPILSHHQLFHHVQTQTQTHHP---P 180
           ++K+    G AK+VGI +CM G  ILA YKGP L   L  H  F+H Q     ++P    
Sbjct: 123 RLKVKSIQGTAKLVGITVCMGGVIILAIYKGPLLKLPLCPH--FYHGQEHPHRNNPGHVS 182

Query: 181 TSQNTWMIGCFFLLVTSVSWGIWFVLQARFLKEYPHPMEFMCAQTVMSTAQTFFVAIAME 240
               +W+ GC  ++ +++ WG+W VLQ R LK YP  + F     ++S+ Q+F +AIA+E
Sbjct: 183 GGSTSWLKGCVLMITSNILWGLWLVLQGRVLKVYPSKLYFTTLHCLLSSIQSFVIAIALE 242

Query: 241 RDPLEWKLGWNVRLYAVLYCGVLVIGVANNAQCWVIREKGPVFQAMTTPLNLMATIIGSL 300
           RD   WKLGWN+RL AV+YCG +V GVA   Q WVI ++GPVF +M TPL+L+ T++ S 
Sbjct: 243 RDISAWKLGWNLRLVAVIYCGFIVTGVAYYLQSWVIEKRGPVFLSMFTPLSLLFTLLSSA 302

Query: 301 LLLSEGIYVGSIIGASLLVMSLYSVLWGKSKELVTAPNDQ 338
           +LL E I +GSI+G  LL++ LY VLWGKS+E   + +D+
Sbjct: 303 ILLCEIISLGSIVGGLLLIIGLYCVLWGKSREEKNSGDDK 340

BLAST of CmoCh03G010660 vs. TAIR10
Match: AT1G43650.1 (AT1G43650.1 nodulin MtN21 /EamA-like transporter family protein)

HSP 1 Score: 236.5 bits (602), Expect = 2.6e-62
Identity = 133/329 (40.43%), Postives = 188/329 (57.14%), Query Frame = 1

Query: 1   MASNNPYIVAFFIQITYAGMSLMSKAAFASGMNTYIFLFYRQAAGTLVLVPLTLLLKGKE 60
           M  +   +   F+QI YAGM L+SK A + G N ++F+FYRQA   L L P    L+  +
Sbjct: 2   MMEHKANMAMVFVQIVYAGMPLLSKVAISQGTNPFVFVFYRQAFAALALSPFAFFLESSK 61

Query: 61  KRPLSLKHLCQIFVISLIGITLALDAYGVAINYTSATLGAAAFNCVPVTTFFFALILRME 120
             PLS   L +IF ISL G+TL+L+ Y VAI  T+AT  AA  N +P  TF  AL+ R+E
Sbjct: 62  SSPLSFILLLKIFFISLCGLTLSLNLYYVAIENTTATFAAATTNAIPSITFVLALLFRLE 121

Query: 121 KVKLNKAAGIAKVVGIMICMAGAAILAFYKGPYLSPILSHHQLFHHVQTQTQTHHPPTSQ 180
            V L K+ G+AKV G M+ M GA + AF KGP          L +H  + T  +    S 
Sbjct: 122 TVTLKKSHGVAKVTGSMVGMLGALVFAFVKGP---------SLINHYNSSTIPNGTVPST 181

Query: 181 NTWMIGCFFLLVTSVSWGIWFVLQARFLKEYPHPMEFMCAQTVMSTAQTFFVAIAMERDP 240
              + G   +L  +  W +W ++Q++ +KEYP  +  +  Q + S  Q+   A+A+ R+P
Sbjct: 182 KNSVKGSITMLAANTCWCLWIIMQSKVMKEYPAKLRLVALQCLFSCIQSAVWAVAVNRNP 241

Query: 241 LEWKLGWNVRLYAVLYCGVLVIGVANNAQCWVIREKGPVFQAMTTPLNLMATIIGSLLLL 300
             WK+ + + L ++ YCG++V G+    Q W I +KGPVF A+ TPL L+ T I S  L 
Sbjct: 242 SVWKIEFGLPLLSMAYCGIMVTGLTYWLQVWAIEKKGPVFTALYTPLALILTCIVSSFLF 301

Query: 301 SEGIYVGSIIGASLLVMSLYSVLWGKSKE 330
            E  Y+GS+ GA LLV  LY  LWGK+KE
Sbjct: 302 KETFYLGSVGGAVLLVCGLYLGLWGKTKE 321

BLAST of CmoCh03G010660 vs. TAIR10
Match: AT1G75500.1 (AT1G75500.1 Walls Are Thin 1)

HSP 1 Score: 195.3 bits (495), Expect = 6.5e-50
Identity = 114/326 (34.97%), Postives = 172/326 (52.76%), Query Frame = 1

Query: 7   YIVAFFIQITYAGMSLMSKAAFASGMNTYIFLFYRQAAGTLVLVPLTLLLKGKEKRPLSL 66
           +I    +Q  YAG  ++S+AA   G++  +F  YR     L+L+P    L+ KE+  ++L
Sbjct: 21  HIAMLTLQFGYAGFHVVSRAALNMGISKLVFPVYRNIIALLLLLPFAYFLEKKERPAITL 80

Query: 67  KHLCQIFVISLIGITLALDAYGVAINYTSATLGAAAFNCVPVTTFFFALILRMEKVKLNK 126
             L Q F ++LIGIT     Y + ++ TS T  ++  N VP  TF  A +LR+EKV++N+
Sbjct: 81  NFLIQFFFLALIGITANQGFYLLGLDNTSPTFASSMQNSVPAITFLMAALLRIEKVRINR 140

Query: 127 AAGIAKVVGIMICMAGAAILAFYKGPYLSPILSHHQLFHHVQTQTQTHHPP---TSQNTW 186
             GI+K++G  +C+AGA+++  YKGP +    SH  L  H+ T       P    +   W
Sbjct: 141 RDGISKILGTALCVAGASVITLYKGPTIYTPASH--LHAHLLTTNSAVLAPLGNAAPKNW 200

Query: 187 MIGCFFLLVTSVSWGIWFVLQARFLKEYPHPMEFMCAQTVMSTAQTFFVAIAMERDPLEW 246
            +GC +L+   +SW  W V QA  LK YP  +            Q   +A   ERD   W
Sbjct: 201 TLGCIYLIGHCLSWSGWLVFQAPVLKSYPARLSVTSYTCFFGIIQFLIIAAFCERDSQAW 260

Query: 247 KLGWNVRLYAVLYCGVLVIGVANNAQCWVIREKGPVFQAMTTPLNLMATIIGSLLLLSEG 306
                  L+ +LY G++  G+A   Q W I   GPVF A+  P+  +   I + + L E 
Sbjct: 261 VFHSGWELFTILYAGIVASGIAFAVQIWCIDRGGPVFVAVYQPVQTLVVAIMASIALGEE 320

Query: 307 IYVGSIIGASLLVMSLYSVLWGKSKE 330
            Y+G IIGA L++  LY VL+GKS+E
Sbjct: 321 FYLGGIIGAVLIIAGLYFVLYGKSEE 344

BLAST of CmoCh03G010660 vs. TAIR10
Match: AT5G07050.1 (AT5G07050.1 nodulin MtN21 /EamA-like transporter family protein)

HSP 1 Score: 189.5 bits (480), Expect = 3.6e-48
Identity = 104/334 (31.14%), Postives = 179/334 (53.59%), Query Frame = 1

Query: 1   MASNNPYIVAFFIQITYAGMSLMSKAAFASGMNTYIFLFYRQAAGTLVLVPLTLLLKGKE 60
           + S+ PY     +Q  YAGM++++K +  +GM+ Y+ + YR A  T V+ P     + K 
Sbjct: 13  LTSSKPYFAMISLQFGYAGMNIITKISLNTGMSHYVLVVYRHAIATAVIAPFAFFFERKA 72

Query: 61  KRPLSLKHLCQIFVISLIGITLALDAYGVAINYTSATLGAAAFNCVPVTTFFFALILRME 120
           +  ++     Q+F++ L+G  +  + Y + + YTS T   A  N +P  TF  A++ RME
Sbjct: 73  QPKITFSIFMQLFILGLLGPVIDQNFYYMGLKYTSPTFSCAMSNMLPAMTFILAVLFRME 132

Query: 121 KVKLNKAAGIAKVVGIMICMAGAAILAFYKGPYLSPILSHHQLFHHVQ----TQTQTHHP 180
            + L K    AK+ G ++ +AGA ++  YKGP +    + +    H+Q      T +   
Sbjct: 133 MLDLKKLWCQAKIAGTVVTVAGAMLMTIYKGPIVELFWTKYM---HIQDSSHANTTSSKN 192

Query: 181 PTSQNTWMIGCFFLLVTSVSWGIWFVLQARFLKEY-PHPMEFMCAQTVMSTAQTFFVAIA 240
            +S   ++ G   L+  +++W   FVLQA+ LK Y  H +        + T Q   V   
Sbjct: 193 SSSDKEFLKGSILLIFATLAWASLFVLQAKILKTYAKHQLSLTTLICFIGTLQAVAVTFV 252

Query: 241 MERDPLEWKLGWNVRLYAVLYCGVLVIGVANNAQCWVIREKGPVFQAMTTPLNLMATIIG 300
           ME +P  W++GW++ L A  Y G++   ++   Q  V++++GPVF    +PL ++   + 
Sbjct: 253 MEHNPSAWRIGWDMNLLAAAYSGIVASSISYYVQGIVMKKRGPVFATAFSPLMMVIVAVM 312

Query: 301 SLLLLSEGIYVGSIIGASLLVMSLYSVLWGKSKE 330
              +L+E I++G +IGA L+V+ LY+VLWGK KE
Sbjct: 313 GSFVLAEKIFLGGVIGAVLIVIGLYAVLWGKQKE 343

BLAST of CmoCh03G010660 vs. TAIR10
Match: AT1G44800.1 (AT1G44800.1 nodulin MtN21 /EamA-like transporter family protein)

HSP 1 Score: 187.6 bits (475), Expect = 1.4e-47
Identity = 110/338 (32.54%), Postives = 184/338 (54.44%), Query Frame = 1

Query: 1   MASNNPYIVAFFIQITYAGMSLMSKAAFASGMNTYIFLFYRQAAGTLVLVPLTLLLKGKE 60
           M    P +    +Q  YAGM +++  +F  GM+ ++   YR    T+V+ P  L+ + K 
Sbjct: 6   MEKIKPILAIISLQFGYAGMYIITMVSFKHGMDHWVLATYRHVVATVVMAPFALMFERKI 65

Query: 61  KRPLSLKHLCQIFVISLIGITLALDAYGVAINYTSATLGAAAFNCVPVTTFFFALILRME 120
           +  ++L    ++  + ++   +  + Y + +  TSA+  +A  N +P  TF  ALI R+E
Sbjct: 66  RPKMTLAIFWRLLALGILEPLMDQNLYYIGLKNTSASYTSAFTNALPAVTFILALIFRLE 125

Query: 121 KVKLNKAAGIAKVVGIMICMAGAAILAFYKGPYLSPILSHHQLFHHVQTQTQTHHPPTSQ 180
            V   K   +AKVVG +I + GA I+  YKGP +  + + H  FH   + T     PT Q
Sbjct: 126 TVNFRKVHSVAKVVGTVITVGGAMIMTLYKGPAIEIVKAAHNSFHGGSSST-----PTGQ 185

Query: 181 NTWMIGCFFLLVTSVSWGIWFVLQARFLKEYPHPMEFMCAQTVMSTAQTFFVAIAMERDP 240
           + W++G   ++ +  +W  +F+LQ+  LK YP  +  +     + T      ++ M RDP
Sbjct: 186 H-WVLGTIAIMGSISTWAAFFILQSYTLKVYPAELSLVTLICGIGTILNAIASLIMVRDP 245

Query: 241 LEWKLGWNVRLYAVLYCGVLVIGVANNAQCWVIREKGPVFQAMTTPLNLMATIIGSLLLL 300
             WK+G +    A +Y GV+  G+A   Q  VI+++GPVF    +P+ ++ T     L+L
Sbjct: 246 SAWKIGMDSGTLAAVYSGVVCSGIAYYIQSIVIKQRGPVFTTSFSPMCMIITAFLGALVL 305

Query: 301 SEGIYVGSIIGASLLVMSLYSVLWGKSKELVTAPNDQQ 339
           +E I++GSIIGA  +V+ LYSV+WGKSK+ V  P D++
Sbjct: 306 AEKIHLGSIIGAVFIVLGLYSVVWGKSKDEVN-PLDEK 336

BLAST of CmoCh03G010660 vs. NCBI nr
Match: gi|659081074|ref|XP_008441136.1| (PREDICTED: WAT1-related protein At5g64700-like [Cucumis melo])

HSP 1 Score: 569.7 bits (1467), Expect = 3.6e-159
Identity = 290/360 (80.56%), Postives = 321/360 (89.17%), Query Frame = 1

Query: 1   MASNNPYIVAFFIQITYAGMSLMSKAAFASGMNTYIFLFYRQAAGTLVLVPLTLLLKGKE 60
           M SN PYIVA FIQIT+AGMSLMSKAAFA+GMNTYIFLFYRQAAG+++LVPLTL+LKGKE
Sbjct: 68  MGSNKPYIVAVFIQITFAGMSLMSKAAFAAGMNTYIFLFYRQAAGSIILVPLTLILKGKE 127

Query: 61  KRPLSLKHLCQIFVISLIGITLALDAYGVAINYTSATLGAAAFNCVPVTTFFFALILRME 120
           KRPL+LKHLCQIFVISLIGITLALDAYGVAINYTSAT+GAAAFNCVPVTTFFFA++LRME
Sbjct: 128 KRPLTLKHLCQIFVISLIGITLALDAYGVAINYTSATIGAAAFNCVPVTTFFFAVLLRME 187

Query: 121 KVKLNKAAGIAKVVGIMICMAGAAILAFYKGPYLSPILSHHQLFHHVQTQTQTHHPPTSQ 180
           KV L KAAGIAKVVGIMIC+AGA+ILA YKGPYL P+ +H QLFHH+ +Q+  HH   S 
Sbjct: 188 KVNLKKAAGIAKVVGIMICIAGASILALYKGPYLKPLFTH-QLFHHIHSQS--HHSLNSH 247

Query: 181 NTWMIGCFFLLVTSVSWGIWFVLQARFLKEYPHPMEFMCAQTVMSTAQTFFVAIAMERDP 240
           N WMIGCFFLLVTSVSWGIWFVLQARFLK YPHPMEFMC QT+MS  Q+F VAI MERDP
Sbjct: 248 NKWMIGCFFLLVTSVSWGIWFVLQARFLKGYPHPMEFMCMQTLMSMVQSFIVAIVMERDP 307

Query: 241 LEWKLGWNVRLYAVLYCGVLVIGVANNAQCWVIREKGPVFQAMTTPLNLMATIIGSLLLL 300
           LEWKLG+N+RL+AVLYCG+LVIG+ANNAQCWVIREKGPVFQAMTTPLNL+ATIIGS  L 
Sbjct: 308 LEWKLGFNIRLFAVLYCGILVIGIANNAQCWVIREKGPVFQAMTTPLNLIATIIGSQFLF 367

Query: 301 SEGIYVGSIIGASLLVMSLYSVLWGKSKELVTAPNDQQPSPPIKQVEESTCRSEVNSTLV 360
            +G Y+GSIIGA LLV SLY VLWGKSKELVT  ND Q SPP K+ ++ST R EVNS+LV
Sbjct: 368 PDGTYLGSIIGAFLLVTSLYCVLWGKSKELVTPSNDVQLSPPQKETDQSTRRPEVNSSLV 424

BLAST of CmoCh03G010660 vs. NCBI nr
Match: gi|778717886|ref|XP_011657775.1| (PREDICTED: WAT1-related protein At5g64700-like isoform X1 [Cucumis sativus])

HSP 1 Score: 568.2 bits (1463), Expect = 1.1e-158
Identity = 288/360 (80.00%), Postives = 318/360 (88.33%), Query Frame = 1

Query: 1   MASNNPYIVAFFIQITYAGMSLMSKAAFASGMNTYIFLFYRQAAGTLVLVPLTLLLKGKE 60
           M SN PYIV FFIQIT+AGMSLMSKAAFA+GMNTYIFLFYRQAAG+++LVPLTL+LKGKE
Sbjct: 1   MGSNKPYIVVFFIQITFAGMSLMSKAAFAAGMNTYIFLFYRQAAGSIILVPLTLILKGKE 60

Query: 61  KRPLSLKHLCQIFVISLIGITLALDAYGVAINYTSATLGAAAFNCVPVTTFFFALILRME 120
           KRPLSLKHLCQIFVISLIGITLALDAYGVAINYTSAT+GAAAFNCVPVTTFFFA++LRME
Sbjct: 61  KRPLSLKHLCQIFVISLIGITLALDAYGVAINYTSATIGAAAFNCVPVTTFFFAVLLRME 120

Query: 121 KVKLNKAAGIAKVVGIMICMAGAAILAFYKGPYLSPILSHHQLFHHVQTQTQTHHPPTSQ 180
           KV L KAAGIAKVVGIMIC+ GA+ILA YKGPYL P+ +H QLFHH+ +Q   HH   S 
Sbjct: 121 KVNLKKAAGIAKVVGIMICITGASILALYKGPYLKPLFTH-QLFHHIHSQP--HHSLNSH 180

Query: 181 NTWMIGCFFLLVTSVSWGIWFVLQARFLKEYPHPMEFMCAQTVMSTAQTFFVAIAMERDP 240
             WMIGCFFLLVTS+SWGIWFVLQARFLK YPHPMEFMC QT+MS  Q+F VAI MERDP
Sbjct: 181 TKWMIGCFFLLVTSISWGIWFVLQARFLKGYPHPMEFMCMQTLMSMVQSFIVAIVMERDP 240

Query: 241 LEWKLGWNVRLYAVLYCGVLVIGVANNAQCWVIREKGPVFQAMTTPLNLMATIIGSLLLL 300
            EWKLG+N+RL+AVLYCG+LVIGVANNAQCWVIREKGPVFQAMTTPLNL+ATIIGS  L 
Sbjct: 241 FEWKLGFNIRLFAVLYCGILVIGVANNAQCWVIREKGPVFQAMTTPLNLIATIIGSQFLF 300

Query: 301 SEGIYVGSIIGASLLVMSLYSVLWGKSKELVTAPNDQQPSPPIKQVEESTCRSEVNSTLV 360
            +G Y+GSIIGA LLV SLY VLWGK+KELVT  ND Q SPP K+ +ESTC +EVNS+LV
Sbjct: 301 PDGTYLGSIIGAFLLVTSLYCVLWGKNKELVTPSNDGQLSPPQKETKESTCPTEVNSSLV 357

BLAST of CmoCh03G010660 vs. NCBI nr
Match: gi|659080822|ref|XP_008440998.1| (PREDICTED: WAT1-related protein At5g64700-like [Cucumis melo])

HSP 1 Score: 523.9 bits (1348), Expect = 2.3e-145
Identity = 270/365 (73.97%), Postives = 311/365 (85.21%), Query Frame = 1

Query: 1   MASNNPYIVAFFIQITYAGMSLMSKAAFASGMNTYIFLFYRQAAGTLVLVPLTLLLKGKE 60
           M +  PYIVA FIQIT+AGMSL+SKAAFA+GMNTYIFLFYRQAAG+L+LVPLT LLKGKE
Sbjct: 1   MDAKKPYIVAIFIQITFAGMSLLSKAAFATGMNTYIFLFYRQAAGSLILVPLTFLLKGKE 60

Query: 61  KRPLSLKHLCQIFVISLIGITLALDAYGVAINYTSATLGAAAFNCVPVTTFFFALILRME 120
           KRPLSLK LC +F ISLIGITLA++AYGVA++YTSATLGAAAFNC+PV+TF FA++ RME
Sbjct: 61  KRPLSLKQLCHVFFISLIGITLAMNAYGVAVDYTSATLGAAAFNCLPVSTFIFAVLFRME 120

Query: 121 KVKLNKAAGIAKVVGIMICMAGAAILAFYKGPYLSPILSHHQLFHHVQTQTQTHHPPTSQ 180
           KV L KAAGIAKV G+MIC+ GAAILAFYKGPYL PI+SH  LFH  +++T      TSQ
Sbjct: 121 KVNLKKAAGIAKVAGMMICVGGAAILAFYKGPYLKPIISH-PLFHIEKSETDI--TATSQ 180

Query: 181 NTWMIGCFFLLVTSVSWGIWFVLQARFLKEYPHPMEFMCAQTVMSTAQTFFVAIAMERDP 240
            +W++GCFFLLV +V WGIWFVLQA+FLK YPHP+EFMCAQTVMS  Q F VAI +ERDP
Sbjct: 181 KSWLLGCFFLLVATVGWGIWFVLQAKFLKGYPHPVEFMCAQTVMSVVQCFVVAIIVERDP 240

Query: 241 LEWKLGWNVRLYAVLYCGVLVIGVANNAQCWVIREKGPVFQAMTTPLNLMATIIGSLLLL 300
            EWKLGWNVRLYAVLYCG+LVIG+ANNAQCWVI+EKGPVFQAM  PLNL+ATIIGS L L
Sbjct: 241 SEWKLGWNVRLYAVLYCGILVIGIANNAQCWVIKEKGPVFQAMMMPLNLVATIIGSQLFL 300

Query: 301 SEGIYVGSIIGASLLVMSLYSVLWGKSKELVTAPNDQQP--SP---PIKQVEESTCRSEV 360
           +EGIY+GS+IGA LLV SLYSVLWGKSKELV  P +Q P  SP   P K+ +E T RS+V
Sbjct: 301 AEGIYLGSVIGAILLVTSLYSVLWGKSKELVVTPTNQDPPSSPDPLPQKEPKEPTNRSQV 360

BLAST of CmoCh03G010660 vs. NCBI nr
Match: gi|449448590|ref|XP_004142049.1| (PREDICTED: WAT1-related protein At5g64700-like [Cucumis sativus])

HSP 1 Score: 516.5 bits (1329), Expect = 3.6e-143
Identity = 264/365 (72.33%), Postives = 308/365 (84.38%), Query Frame = 1

Query: 1   MASNNPYIVAFFIQITYAGMSLMSKAAFASGMNTYIFLFYRQAAGTLVLVPLTLLLKGKE 60
           M S  PY+VA FIQIT+AGMSL+SKAAFA+GMNTYIFLFYRQAAG+L+L+PLTLLLKGKE
Sbjct: 1   MDSKKPYLVAIFIQITFAGMSLLSKAAFATGMNTYIFLFYRQAAGSLILIPLTLLLKGKE 60

Query: 61  KRPLSLKHLCQIFVISLIGITLALDAYGVAINYTSATLGAAAFNCVPVTTFFFALILRME 120
           KRPLS K LCQ F ISLIGITLA++AYGVA++YTSATLGAAAFNC+PV+TF FA++ RME
Sbjct: 61  KRPLSFKQLCQCFFISLIGITLAMNAYGVAVDYTSATLGAAAFNCLPVSTFIFAVLFRME 120

Query: 121 KVKLNKAAGIAKVVGIMICMAGAAILAFYKGPYLSPILSHHQLFHHVQTQTQTHHPPTSQ 180
           +V L KAAGIAKV G+MIC+ GAAILAFYKGPYL PI+SH  +FH  +++T      TSQ
Sbjct: 121 RVNLKKAAGIAKVGGMMICVGGAAILAFYKGPYLKPIISH-PIFHIEESETDI--TTTSQ 180

Query: 181 NTWMIGCFFLLVTSVSWGIWFVLQARFLKEYPHPMEFMCAQTVMSTAQTFFVAIAMERDP 240
            +W++GCFFLLV +V WGIWFV QA+FLK YPHP+EFMCAQTVMS  Q F VAI +ERDP
Sbjct: 181 KSWLLGCFFLLVATVGWGIWFVFQAKFLKGYPHPVEFMCAQTVMSVVQCFVVAIIVERDP 240

Query: 241 LEWKLGWNVRLYAVLYCGVLVIGVANNAQCWVIREKGPVFQAMTTPLNLMATIIGSLLLL 300
            EWKLGWNVRLYAVLYCG+LVIG+ANNAQCWVI+EKGPVFQAM  PLNL+ATIIGS L L
Sbjct: 241 SEWKLGWNVRLYAVLYCGILVIGIANNAQCWVIKEKGPVFQAMMMPLNLVATIIGSQLFL 300

Query: 301 SEGIYVGSIIGASLLVMSLYSVLWGKSKELVTAPNDQQ-----PSPPIKQVEESTCRSEV 360
           +EGIY+GS+IGA LLV SLYSVLWGK+KELV  P +Q+      S P K+ EE   RS+V
Sbjct: 301 AEGIYLGSVIGAILLVTSLYSVLWGKNKELVVTPTNQERPSSPDSLPQKESEEPANRSQV 360

BLAST of CmoCh03G010660 vs. NCBI nr
Match: gi|778717898|ref|XP_011657778.1| (PREDICTED: WAT1-related protein At5g64700-like isoform X1 [Cucumis sativus])

HSP 1 Score: 473.0 bits (1216), Expect = 4.6e-130
Identity = 245/362 (67.68%), Postives = 288/362 (79.56%), Query Frame = 1

Query: 1   MASNNPYIVAFFIQITYAGMSLMSKAAFASGMNTYIFLFYRQAAGTLVLVPLTLLLKGKE 60
           M S   YIVA F QI YAGMSL SK AFA+GMNTY+FLFYRQAAG+L+L+PLTLLLKGKE
Sbjct: 1   MGSKKFYIVAIFTQIIYAGMSLSSKVAFATGMNTYVFLFYRQAAGSLILIPLTLLLKGKE 60

Query: 61  KRPLSLKHLCQIFVISLIGITLALDAYGVAINYTSATLGAAAFNCVPVTTFFFALILRME 120
           KRPLSLK LC  F ISLIGITL ++AY V I+YTS T GAAA NC+P++TF FA+  RME
Sbjct: 61  KRPLSLKQLCHAFFISLIGITLTMNAYDVGIDYTSPTFGAAAINCIPISTFIFAVTFRME 120

Query: 121 KVKLNKAAGIAKVVGIMICMAGAAILAFYKGPYLSPILSHHQLFHHVQTQTQTHHPPTSQ 180
           KV L KA+GIAKV G+MIC+ GAAILAFYKGPYL PI+SH  +FH  +++T      TSQ
Sbjct: 121 KVNLKKASGIAKVGGMMICVGGAAILAFYKGPYLKPIISH-PIFHFEESETDI--TTTSQ 180

Query: 181 NTWMIGCFFLLVTSVSWGIWFVLQARFLKEYPHPMEFMCAQTVMSTAQTFFVAIAMERDP 240
            +WM+GCF LLV ++ WGIW+V QA FLK+YPHP+E MC QTVMS  Q F VAI +ERD 
Sbjct: 181 KSWMLGCFILLVATMGWGIWYVFQAMFLKDYPHPVELMCTQTVMSVVQCFVVAIIVERDH 240

Query: 241 LEWKLGWNVRLYAVLYCGVLVIGVANNAQCWVIREKGPVFQAMTTPLNLMATIIGSLLLL 300
            EWKL WNVRLYA+LYCG+LVIG+ANNAQCWVI+E GPVF +M  P+NL+ATIIGS L L
Sbjct: 241 SEWKLDWNVRLYAILYCGILVIGIANNAQCWVIKETGPVFPSMMMPINLVATIIGSQLFL 300

Query: 301 SEGIYVGSIIGASLLVMSLYSVLWGKSKELVTAP-NDQQP-SP---PIKQVEESTCRSEV 358
           +EGIY+GS+IGA LLV+SLYSVLWGKSKELV  P N  QP SP   P K+ EE   RS+V
Sbjct: 301 AEGIYLGSVIGAILLVISLYSVLWGKSKELVDTPTNQDQPFSPDFLPQKESEEQDSRSQV 359

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
WTR45_ARATH8.8e-8146.47WAT1-related protein At5g64700 OS=Arabidopsis thaliana GN=At5g64700 PE=2 SV=1[more]
WTR7_ARATH4.5e-6140.43WAT1-related protein At1g43650 OS=Arabidopsis thaliana GN=At1g43650 PE=2 SV=1[more]
WAT1_ARATH1.2e-4834.97Protein WALLS ARE THIN 1 OS=Arabidopsis thaliana GN=WAT1 PE=1 SV=1[more]
WTR38_ARATH6.4e-4731.14WAT1-related protein At5g07050 OS=Arabidopsis thaliana GN=At5g07050 PE=2 SV=1[more]
WTR8_ARATH2.4e-4632.54WAT1-related protein At1g44800 OS=Arabidopsis thaliana GN=At1g44800 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0KFA3_CUCSA7.3e-15980.00WAT1-related protein OS=Cucumis sativus GN=Csa_6G485160 PE=3 SV=1[more]
A0A0A0KJ33_CUCSA2.5e-14372.33WAT1-related protein OS=Cucumis sativus GN=Csa_6G485670 PE=3 SV=1[more]
A0A0A0KKW0_CUCSA3.2e-13067.68WAT1-related protein OS=Cucumis sativus GN=Csa_6G485170 PE=3 SV=1[more]
A0A0A0KHV4_CUCSA2.0e-10362.46WAT1-related protein OS=Cucumis sativus GN=Csa_6G485150 PE=3 SV=1[more]
M5X3D1_PRUPE9.7e-10353.61WAT1-related protein OS=Prunus persica GN=PRUPE_ppa015904mg PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT5G64700.15.0e-8246.47 nodulin MtN21 /EamA-like transporter family protein[more]
AT1G43650.12.6e-6240.43 nodulin MtN21 /EamA-like transporter family protein[more]
AT1G75500.16.5e-5034.97 Walls Are Thin 1[more]
AT5G07050.13.6e-4831.14 nodulin MtN21 /EamA-like transporter family protein[more]
AT1G44800.11.4e-4732.54 nodulin MtN21 /EamA-like transporter family protein[more]
Match NameE-valueIdentityDescription
gi|659081074|ref|XP_008441136.1|3.6e-15980.56PREDICTED: WAT1-related protein At5g64700-like [Cucumis melo][more]
gi|778717886|ref|XP_011657775.1|1.1e-15880.00PREDICTED: WAT1-related protein At5g64700-like isoform X1 [Cucumis sativus][more]
gi|659080822|ref|XP_008440998.1|2.3e-14573.97PREDICTED: WAT1-related protein At5g64700-like [Cucumis melo][more]
gi|449448590|ref|XP_004142049.1|3.6e-14372.33PREDICTED: WAT1-related protein At5g64700-like [Cucumis sativus][more]
gi|778717898|ref|XP_011657778.1|4.6e-13067.68PREDICTED: WAT1-related protein At5g64700-like isoform X1 [Cucumis sativus][more]
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR000620EamA_dom
Vocabulary: Cellular Component
TermDefinition
GO:0016021integral component of membrane
GO:0016020membrane
Vocabulary: Molecular Function
TermDefinition
GO:0022857transmembrane transporter activity
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0055085 transmembrane transport
biological_process GO:0008150 biological_process
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0016020 membrane
molecular_function GO:0022857 transmembrane transporter activity

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmoCh03G010660.1CmoCh03G010660.1mRNA


Analysis Name: InterPro Annotations of Cucurbita moschata
Date Performed: 2017-05-19
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000620EamA domainPFAMPF00892EamAcoord: 184..323
score: 2.0E-10coord: 6..134
score: 1.1
NoneNo IPR availablePANTHERPTHR31218:SF15SUBFAMILY NOT NAMEDcoord: 1..329
score: 7.7E
NoneNo IPR availableunknownSSF103481Multidrug resistance efflux transporter EmrEcoord: 40..149
score: 8.

The following gene(s) are paralogous to this gene:
GeneParalogueOrganismBlock
CmoCh03G010660CmoCh02G005910Cucurbita moschata (Rifu)cmocmoB431
CmoCh03G010660CmoCh07G008040Cucurbita moschata (Rifu)cmocmoB455
The following block(s) are covering this gene:
GeneOrganismBlock
CmoCh03G010660Cucumber (Chinese Long) v3cmocucB0793
CmoCh03G010660Wax gourdcmowgoB0777
CmoCh03G010660Cucurbita pepo (Zucchini)cmocpeB636
CmoCh03G010660Silver-seed gourdcarcmoB1424