CmaCh03G010720 (gene) Cucurbita maxima (Rimu)

NameCmaCh03G010720
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu))
DescriptionNodulin MtN21/EamA-like transporter family protein
LocationCma_Chr03 : 7376133 .. 7378102 (+)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CATCACCAAATATGGCTTCCAACAACCCTTACATCGTTGCATTCTTCATCCAAATCACCTACGCCGGTATGAGCCTCATGTCCAAGGCCGCCTTCGCCTCTGGTATGAACACTTATATCTTCCTCTTCTATCGTCAAGCTGCTGGTACTCTTGTTTTGGTCCCGCTCACCCTACTCTTAAAAGGGTACGTTTCGTTTTCAATGTTTTTTTTTTTTTTTTCGATTTACCAATATAAACATAGCTCATAGCGGATCATTTTGTTGCAAATATATTTCAGGAAGGAGAAGAGGCCGTTGTCTTTGAAGCATTTGTGCCAGATTTTTGTCATTTCATTGATAGGGTGTGTATTAATATTTATCAGTAATGTTAGGAATTATAACTCTCCGCAATTGTATGATATTGTCCATTTTGAGTATAAGTTCTTTCGCTTAGTGTTTTAGTTTCCCCAAAAGACCTCATAGCAATGGAGATATTGTATTCCCTACTTATCAACTCATAATCATTCCCTTAATTAGCCAATATCGGACTCCCACCTATCTTCTCCTCGAACAAAGTACATCATAGAGCCTCTCTTGAGGCCTATGGAGCCCTCAAACAACCTCCCTTTAATTGAGGCTCGACTCAATTCTCTGGAGACCTCGAACAAAGTCCCAACTTTTTTCTTTGACATTTGAGGATTCTATTAACACGATTAAATTAAGAGATGATTCTGATATCATATTAAGAATTACGACTCTTCAATGTAGGACTCACTCCCAACGATATATCAACTTTTTATTTTATTCAAAGAATCTTATTATAGTTTCAATTATGAATTGGGTTTAGGATCACTCTAGCATTGGATGCATATGGAGTAGCCATTAACTATACGTCGGCAACATTGGGCGCCGCAGCTTTCAATTGCGTTCCTGTCACCACATTCTTCTTCGCACTCATACTAAGAATGGAGAAGGTGAAGCTAAATAAAGCAGCCGGGATAGCAAAGGTGGTGGGCATAATGATATGCATGGCGGGCGCCGCCATTCTAGCTTTCTACAAAGGCCCATATTTAAGCCCAATTTTGAGCCACCACCAACTATTCCACCACGTCCAAACCCAAACACAAACCCACCACCCTCCTACCTCCCAAAACACATGGATGATCGGCTGCTTCTTCCTCCTCGTCACCAGCGTCTCTTGGGGGATCTGGTTCGTTCTCCAGGCTCGATTCTTGAAGGAGTACCCGCACCCGATGGAGTTCATGTGCGCACAGACCGTGATGAGCACGGCTCAGACCTTCGTCGTAGCCATTGCCATGGAAAGAGACCCTTTGGAGTGGAAATTGGGCTGGAATGTTCGACTCTACGCTGTGCTCTACTGTGTAAGCTCAATCAAATAACGCGTTTTAGTAATAACGACTCAGTCATTGAACTTACCCAAAAATGTAATATCTACTTATAAATCCATTATTATTTCTTAAATTAGCTTGATATCTGGTGCAGGGTGTGCTAGTCATTGGTGTGGCAAATAATGCCCAATGTTGGGTCATCAGAGAGAAGGGTCCAGTTTTTCAAGCCATGACGACGCCGTTGAACTTAATGGCTACAATAATTGGTTCTCTATTGCTACTTTCTGAAGGAATTTACGTGGGAAGGTAATAAATTACGGTAATTTAAGCTGTAAATTTTTGTGTTAAAAGTTACTGAATTTAGTGTTGTTGTTGTTGGGATTACAGTATAATAGGAGCATCGTTGCTGGTCATGAGTCTTTACAGTGTTTTGTGGGGTAAAAGCAAGGAGCTTGTCACTGCTCCAAACGACCAACAACCGTCTCCACCACTAAAACAGGCTGATGAGTCGACATGTCGTTCTGAAGTCAACTCCACGTTAGTCTAAGCAAAAGCTGTGATGAACTTGTTCGTGATATTTAATTTCTTCTCTAAAATTGTATTTTGGTTACTAAATGCAATACAATTCCCCTATCAATTTCCA

mRNA sequence

CATCACCAAATATGGCTTCCAACAACCCTTACATCGTTGCATTCTTCATCCAAATCACCTACGCCGGTATGAGCCTCATGTCCAAGGCCGCCTTCGCCTCTGGTATGAACACTTATATCTTCCTCTTCTATCGTCAAGCTGCTGGTACTCTTGTTTTGGTCCCGCTCACCCTACTCTTAAAAGGGAAGGAGAAGAGGCCGTTGTCTTTGAAGCATTTGTGCCAGATTTTTGTCATTTCATTGATAGGGATCACTCTAGCATTGGATGCATATGGAGTAGCCATTAACTATACGTCGGCAACATTGGGCGCCGCAGCTTTCAATTGCGTTCCTGTCACCACATTCTTCTTCGCACTCATACTAAGAATGGAGAAGGTGAAGCTAAATAAAGCAGCCGGGATAGCAAAGGTGGTGGGCATAATGATATGCATGGCGGGCGCCGCCATTCTAGCTTTCTACAAAGGCCCATATTTAAGCCCAATTTTGAGCCACCACCAACTATTCCACCACGTCCAAACCCAAACACAAACCCACCACCCTCCTACCTCCCAAAACACATGGATGATCGGCTGCTTCTTCCTCCTCGTCACCAGCGTCTCTTGGGGGATCTGGTTCGTTCTCCAGGCTCGATTCTTGAAGGAGTACCCGCACCCGATGGAGTTCATGTGCGCACAGACCGTGATGAGCACGGCTCAGACCTTCGTCGTAGCCATTGCCATGGAAAGAGACCCTTTGGAGTGGAAATTGGGCTGGAATGTTCGACTCTACGCTGTGCTCTACTGTGGTGTGCTAGTCATTGGTGTGGCAAATAATGCCCAATGTTGGGTCATCAGAGAGAAGGGTCCAGTTTTTCAAGCCATGACGACGCCGTTGAACTTAATGGCTACAATAATTGGTTCTCTATTGCTACTTTCTGAAGGAATTTACGTGGGAAGTATAATAGGAGCATCGTTGCTGGTCATGAGTCTTTACAGTGTTTTGTGGGGTAAAAGCAAGGAGCTTGTCACTGCTCCAAACGACCAACAACCGTCTCCACCACTAAAACAGGCTGATGAGTCGACATGTCGTTCTGAAGTCAACTCCACGTTAGTCTAAGCAAAAGCTGTGATGAACTTGTTCGTGATATTTAATTTCTTCTCTAAAATTGTATTTTGGTTACTAAATGCAATACAATTCCCCTATCAATTTCCA

Coding sequence (CDS)

ATGGCTTCCAACAACCCTTACATCGTTGCATTCTTCATCCAAATCACCTACGCCGGTATGAGCCTCATGTCCAAGGCCGCCTTCGCCTCTGGTATGAACACTTATATCTTCCTCTTCTATCGTCAAGCTGCTGGTACTCTTGTTTTGGTCCCGCTCACCCTACTCTTAAAAGGGAAGGAGAAGAGGCCGTTGTCTTTGAAGCATTTGTGCCAGATTTTTGTCATTTCATTGATAGGGATCACTCTAGCATTGGATGCATATGGAGTAGCCATTAACTATACGTCGGCAACATTGGGCGCCGCAGCTTTCAATTGCGTTCCTGTCACCACATTCTTCTTCGCACTCATACTAAGAATGGAGAAGGTGAAGCTAAATAAAGCAGCCGGGATAGCAAAGGTGGTGGGCATAATGATATGCATGGCGGGCGCCGCCATTCTAGCTTTCTACAAAGGCCCATATTTAAGCCCAATTTTGAGCCACCACCAACTATTCCACCACGTCCAAACCCAAACACAAACCCACCACCCTCCTACCTCCCAAAACACATGGATGATCGGCTGCTTCTTCCTCCTCGTCACCAGCGTCTCTTGGGGGATCTGGTTCGTTCTCCAGGCTCGATTCTTGAAGGAGTACCCGCACCCGATGGAGTTCATGTGCGCACAGACCGTGATGAGCACGGCTCAGACCTTCGTCGTAGCCATTGCCATGGAAAGAGACCCTTTGGAGTGGAAATTGGGCTGGAATGTTCGACTCTACGCTGTGCTCTACTGTGGTGTGCTAGTCATTGGTGTGGCAAATAATGCCCAATGTTGGGTCATCAGAGAGAAGGGTCCAGTTTTTCAAGCCATGACGACGCCGTTGAACTTAATGGCTACAATAATTGGTTCTCTATTGCTACTTTCTGAAGGAATTTACGTGGGAAGTATAATAGGAGCATCGTTGCTGGTCATGAGTCTTTACAGTGTTTTGTGGGGTAAAAGCAAGGAGCTTGTCACTGCTCCAAACGACCAACAACCGTCTCCACCACTAAAACAGGCTGATGAGTCGACATGTCGTTCTGAAGTCAACTCCACGTTAGTCTAA

Protein sequence

MASNNPYIVAFFIQITYAGMSLMSKAAFASGMNTYIFLFYRQAAGTLVLVPLTLLLKGKEKRPLSLKHLCQIFVISLIGITLALDAYGVAINYTSATLGAAAFNCVPVTTFFFALILRMEKVKLNKAAGIAKVVGIMICMAGAAILAFYKGPYLSPILSHHQLFHHVQTQTQTHHPPTSQNTWMIGCFFLLVTSVSWGIWFVLQARFLKEYPHPMEFMCAQTVMSTAQTFVVAIAMERDPLEWKLGWNVRLYAVLYCGVLVIGVANNAQCWVIREKGPVFQAMTTPLNLMATIIGSLLLLSEGIYVGSIIGASLLVMSLYSVLWGKSKELVTAPNDQQPSPPLKQADESTCRSEVNSTLV
BLAST of CmaCh03G010720 vs. Swiss-Prot
Match: WTR45_ARATH (WAT1-related protein At5g64700 OS=Arabidopsis thaliana GN=At5g64700 PE=2 SV=1)

HSP 1 Score: 303.5 bits (776), Expect = 3.0e-81
Identity = 159/340 (46.76%), Postives = 220/340 (64.71%), Query Frame = 1

Query: 1   MASNNPYIVAFFIQITYAGMSLMSKAAFASGMNTYIFLFYRQAAGTLVLVPLTLLLKGKE 60
           M S  PY++   IQ+ Y  M L+SKA F  GMNT++F+FYRQA  T+ L PL    + K 
Sbjct: 3   MESKKPYLMVTIIQVIYTIMFLISKAVFNGGMNTFVFVFYRQAFATIFLAPLAFFFERKS 62

Query: 61  KRPLSLKHLCQIFVISLIGITLALDAYGVAINYTSATLGAAAFNCVPVTTFFFALILRME 120
             PLS     +IF++SL G+TL+LD  G+A++YTSATL AA    +P  TFF AL+  ME
Sbjct: 63  APPLSFVTFIKIFMLSLFGVTLSLDLNGIALSYTSATLAAATTASLPAITFFLALLFGME 122

Query: 121 KVKLNKAAGIAKVVGIMICMAGAAILAFYKGPYLSPILSHHQLFHHVQTQTQTHHP---P 180
           ++K+    G AK+VGI +CM G  ILA YKGP L   L  H  F+H Q     ++P    
Sbjct: 123 RLKVKSIQGTAKLVGITVCMGGVIILAIYKGPLLKLPLCPH--FYHGQEHPHRNNPGHVS 182

Query: 181 TSQNTWMIGCFFLLVTSVSWGIWFVLQARFLKEYPHPMEFMCAQTVMSTAQTFVVAIAME 240
               +W+ GC  ++ +++ WG+W VLQ R LK YP  + F     ++S+ Q+FV+AIA+E
Sbjct: 183 GGSTSWLKGCVLMITSNILWGLWLVLQGRVLKVYPSKLYFTTLHCLLSSIQSFVIAIALE 242

Query: 241 RDPLEWKLGWNVRLYAVLYCGVLVIGVANNAQCWVIREKGPVFQAMTTPLNLMATIIGSL 300
           RD   WKLGWN+RL AV+YCG +V GVA   Q WVI ++GPVF +M TPL+L+ T++ S 
Sbjct: 243 RDISAWKLGWNLRLVAVIYCGFIVTGVAYYLQSWVIEKRGPVFLSMFTPLSLLFTLLSSA 302

Query: 301 LLLSEGIYVGSIIGASLLVMSLYSVLWGKSKELVTAPNDQ 338
           +LL E I +GSI+G  LL++ LY VLWGKS+E   + +D+
Sbjct: 303 ILLCEIISLGSIVGGLLLIIGLYCVLWGKSREEKNSGDDK 340

BLAST of CmaCh03G010720 vs. Swiss-Prot
Match: WTR7_ARATH (WAT1-related protein At1g43650 OS=Arabidopsis thaliana GN=At1g43650 PE=2 SV=1)

HSP 1 Score: 238.0 bits (606), Expect = 1.6e-61
Identity = 134/329 (40.73%), Postives = 189/329 (57.45%), Query Frame = 1

Query: 1   MASNNPYIVAFFIQITYAGMSLMSKAAFASGMNTYIFLFYRQAAGTLVLVPLTLLLKGKE 60
           M  +   +   F+QI YAGM L+SK A + G N ++F+FYRQA   L L P    L+  +
Sbjct: 2   MMEHKANMAMVFVQIVYAGMPLLSKVAISQGTNPFVFVFYRQAFAALALSPFAFFLESSK 61

Query: 61  KRPLSLKHLCQIFVISLIGITLALDAYGVAINYTSATLGAAAFNCVPVTTFFFALILRME 120
             PLS   L +IF ISL G+TL+L+ Y VAI  T+AT  AA  N +P  TF  AL+ R+E
Sbjct: 62  SSPLSFILLLKIFFISLCGLTLSLNLYYVAIENTTATFAAATTNAIPSITFVLALLFRLE 121

Query: 121 KVKLNKAAGIAKVVGIMICMAGAAILAFYKGPYLSPILSHHQLFHHVQTQTQTHHPPTSQ 180
            V L K+ G+AKV G M+ M GA + AF KGP          L +H  + T  +    S 
Sbjct: 122 TVTLKKSHGVAKVTGSMVGMLGALVFAFVKGP---------SLINHYNSSTIPNGTVPST 181

Query: 181 NTWMIGCFFLLVTSVSWGIWFVLQARFLKEYPHPMEFMCAQTVMSTAQTFVVAIAMERDP 240
              + G   +L  +  W +W ++Q++ +KEYP  +  +  Q + S  Q+ V A+A+ R+P
Sbjct: 182 KNSVKGSITMLAANTCWCLWIIMQSKVMKEYPAKLRLVALQCLFSCIQSAVWAVAVNRNP 241

Query: 241 LEWKLGWNVRLYAVLYCGVLVIGVANNAQCWVIREKGPVFQAMTTPLNLMATIIGSLLLL 300
             WK+ + + L ++ YCG++V G+    Q W I +KGPVF A+ TPL L+ T I S  L 
Sbjct: 242 SVWKIEFGLPLLSMAYCGIMVTGLTYWLQVWAIEKKGPVFTALYTPLALILTCIVSSFLF 301

Query: 301 SEGIYVGSIIGASLLVMSLYSVLWGKSKE 330
            E  Y+GS+ GA LLV  LY  LWGK+KE
Sbjct: 302 KETFYLGSVGGAVLLVCGLYLGLWGKTKE 321

BLAST of CmaCh03G010720 vs. Swiss-Prot
Match: WAT1_ARATH (Protein WALLS ARE THIN 1 OS=Arabidopsis thaliana GN=WAT1 PE=1 SV=1)

HSP 1 Score: 196.1 bits (497), Expect = 6.8e-49
Identity = 114/326 (34.97%), Postives = 173/326 (53.07%), Query Frame = 1

Query: 7   YIVAFFIQITYAGMSLMSKAAFASGMNTYIFLFYRQAAGTLVLVPLTLLLKGKEKRPLSL 66
           +I    +Q  YAG  ++S+AA   G++  +F  YR     L+L+P    L+ KE+  ++L
Sbjct: 21  HIAMLTLQFGYAGFHVVSRAALNMGISKLVFPVYRNIIALLLLLPFAYFLEKKERPAITL 80

Query: 67  KHLCQIFVISLIGITLALDAYGVAINYTSATLGAAAFNCVPVTTFFFALILRMEKVKLNK 126
             L Q F ++LIGIT     Y + ++ TS T  ++  N VP  TF  A +LR+EKV++N+
Sbjct: 81  NFLIQFFFLALIGITANQGFYLLGLDNTSPTFASSMQNSVPAITFLMAALLRIEKVRINR 140

Query: 127 AAGIAKVVGIMICMAGAAILAFYKGPYLSPILSHHQLFHHVQTQTQTHHPP---TSQNTW 186
             GI+K++G  +C+AGA+++  YKGP +    SH  L  H+ T       P    +   W
Sbjct: 141 RDGISKILGTALCVAGASVITLYKGPTIYTPASH--LHAHLLTTNSAVLAPLGNAAPKNW 200

Query: 187 MIGCFFLLVTSVSWGIWFVLQARFLKEYPHPMEFMCAQTVMSTAQTFVVAIAMERDPLEW 246
            +GC +L+   +SW  W V QA  LK YP  +            Q  ++A   ERD   W
Sbjct: 201 TLGCIYLIGHCLSWSGWLVFQAPVLKSYPARLSVTSYTCFFGIIQFLIIAAFCERDSQAW 260

Query: 247 KLGWNVRLYAVLYCGVLVIGVANNAQCWVIREKGPVFQAMTTPLNLMATIIGSLLLLSEG 306
                  L+ +LY G++  G+A   Q W I   GPVF A+  P+  +   I + + L E 
Sbjct: 261 VFHSGWELFTILYAGIVASGIAFAVQIWCIDRGGPVFVAVYQPVQTLVVAIMASIALGEE 320

Query: 307 IYVGSIIGASLLVMSLYSVLWGKSKE 330
            Y+G IIGA L++  LY VL+GKS+E
Sbjct: 321 FYLGGIIGAVLIIAGLYFVLYGKSEE 344

BLAST of CmaCh03G010720 vs. Swiss-Prot
Match: WTR38_ARATH (WAT1-related protein At5g07050 OS=Arabidopsis thaliana GN=At5g07050 PE=2 SV=1)

HSP 1 Score: 189.9 bits (481), Expect = 4.9e-47
Identity = 104/334 (31.14%), Postives = 179/334 (53.59%), Query Frame = 1

Query: 1   MASNNPYIVAFFIQITYAGMSLMSKAAFASGMNTYIFLFYRQAAGTLVLVPLTLLLKGKE 60
           + S+ PY     +Q  YAGM++++K +  +GM+ Y+ + YR A  T V+ P     + K 
Sbjct: 13  LTSSKPYFAMISLQFGYAGMNIITKISLNTGMSHYVLVVYRHAIATAVIAPFAFFFERKA 72

Query: 61  KRPLSLKHLCQIFVISLIGITLALDAYGVAINYTSATLGAAAFNCVPVTTFFFALILRME 120
           +  ++     Q+F++ L+G  +  + Y + + YTS T   A  N +P  TF  A++ RME
Sbjct: 73  QPKITFSIFMQLFILGLLGPVIDQNFYYMGLKYTSPTFSCAMSNMLPAMTFILAVLFRME 132

Query: 121 KVKLNKAAGIAKVVGIMICMAGAAILAFYKGPYLSPILSHHQLFHHVQ----TQTQTHHP 180
            + L K    AK+ G ++ +AGA ++  YKGP +    + +    H+Q      T +   
Sbjct: 133 MLDLKKLWCQAKIAGTVVTVAGAMLMTIYKGPIVELFWTKYM---HIQDSSHANTTSSKN 192

Query: 181 PTSQNTWMIGCFFLLVTSVSWGIWFVLQARFLKEY-PHPMEFMCAQTVMSTAQTFVVAIA 240
            +S   ++ G   L+  +++W   FVLQA+ LK Y  H +        + T Q   V   
Sbjct: 193 SSSDKEFLKGSILLIFATLAWASLFVLQAKILKTYAKHQLSLTTLICFIGTLQAVAVTFV 252

Query: 241 MERDPLEWKLGWNVRLYAVLYCGVLVIGVANNAQCWVIREKGPVFQAMTTPLNLMATIIG 300
           ME +P  W++GW++ L A  Y G++   ++   Q  V++++GPVF    +PL ++   + 
Sbjct: 253 MEHNPSAWRIGWDMNLLAAAYSGIVASSISYYVQGIVMKKRGPVFATAFSPLMMVIVAVM 312

Query: 301 SLLLLSEGIYVGSIIGASLLVMSLYSVLWGKSKE 330
              +L+E I++G +IGA L+V+ LY+VLWGK KE
Sbjct: 313 GSFVLAEKIFLGGVIGAVLIVIGLYAVLWGKQKE 343

BLAST of CmaCh03G010720 vs. Swiss-Prot
Match: WTR8_ARATH (WAT1-related protein At1g44800 OS=Arabidopsis thaliana GN=At1g44800 PE=1 SV=1)

HSP 1 Score: 188.3 bits (477), Expect = 1.4e-46
Identity = 110/338 (32.54%), Postives = 185/338 (54.73%), Query Frame = 1

Query: 1   MASNNPYIVAFFIQITYAGMSLMSKAAFASGMNTYIFLFYRQAAGTLVLVPLTLLLKGKE 60
           M    P +    +Q  YAGM +++  +F  GM+ ++   YR    T+V+ P  L+ + K 
Sbjct: 6   MEKIKPILAIISLQFGYAGMYIITMVSFKHGMDHWVLATYRHVVATVVMAPFALMFERKI 65

Query: 61  KRPLSLKHLCQIFVISLIGITLALDAYGVAINYTSATLGAAAFNCVPVTTFFFALILRME 120
           +  ++L    ++  + ++   +  + Y + +  TSA+  +A  N +P  TF  ALI R+E
Sbjct: 66  RPKMTLAIFWRLLALGILEPLMDQNLYYIGLKNTSASYTSAFTNALPAVTFILALIFRLE 125

Query: 121 KVKLNKAAGIAKVVGIMICMAGAAILAFYKGPYLSPILSHHQLFHHVQTQTQTHHPPTSQ 180
            V   K   +AKVVG +I + GA I+  YKGP +  + + H  FH   + T     PT Q
Sbjct: 126 TVNFRKVHSVAKVVGTVITVGGAMIMTLYKGPAIEIVKAAHNSFHGGSSST-----PTGQ 185

Query: 181 NTWMIGCFFLLVTSVSWGIWFVLQARFLKEYPHPMEFMCAQTVMSTAQTFVVAIAMERDP 240
           + W++G   ++ +  +W  +F+LQ+  LK YP  +  +     + T    + ++ M RDP
Sbjct: 186 H-WVLGTIAIMGSISTWAAFFILQSYTLKVYPAELSLVTLICGIGTILNAIASLIMVRDP 245

Query: 241 LEWKLGWNVRLYAVLYCGVLVIGVANNAQCWVIREKGPVFQAMTTPLNLMATIIGSLLLL 300
             WK+G +    A +Y GV+  G+A   Q  VI+++GPVF    +P+ ++ T     L+L
Sbjct: 246 SAWKIGMDSGTLAAVYSGVVCSGIAYYIQSIVIKQRGPVFTTSFSPMCMIITAFLGALVL 305

Query: 301 SEGIYVGSIIGASLLVMSLYSVLWGKSKELVTAPNDQQ 339
           +E I++GSIIGA  +V+ LYSV+WGKSK+ V  P D++
Sbjct: 306 AEKIHLGSIIGAVFIVLGLYSVVWGKSKDEVN-PLDEK 336

BLAST of CmaCh03G010720 vs. TrEMBL
Match: A0A0A0KFA3_CUCSA (WAT1-related protein OS=Cucumis sativus GN=Csa_6G485160 PE=3 SV=1)

HSP 1 Score: 568.5 bits (1464), Expect = 5.6e-159
Identity = 288/360 (80.00%), Postives = 318/360 (88.33%), Query Frame = 1

Query: 1   MASNNPYIVAFFIQITYAGMSLMSKAAFASGMNTYIFLFYRQAAGTLVLVPLTLLLKGKE 60
           M SN PYIV FFIQIT+AGMSLMSKAAFA+GMNTYIFLFYRQAAG+++LVPLTL+LKGKE
Sbjct: 1   MGSNKPYIVVFFIQITFAGMSLMSKAAFAAGMNTYIFLFYRQAAGSIILVPLTLILKGKE 60

Query: 61  KRPLSLKHLCQIFVISLIGITLALDAYGVAINYTSATLGAAAFNCVPVTTFFFALILRME 120
           KRPLSLKHLCQIFVISLIGITLALDAYGVAINYTSAT+GAAAFNCVPVTTFFFA++LRME
Sbjct: 61  KRPLSLKHLCQIFVISLIGITLALDAYGVAINYTSATIGAAAFNCVPVTTFFFAVLLRME 120

Query: 121 KVKLNKAAGIAKVVGIMICMAGAAILAFYKGPYLSPILSHHQLFHHVQTQTQTHHPPTSQ 180
           KV L KAAGIAKVVGIMIC+ GA+ILA YKGPYL P+ +H QLFHH+ +Q   HH   S 
Sbjct: 121 KVNLKKAAGIAKVVGIMICITGASILALYKGPYLKPLFTH-QLFHHIHSQP--HHSLNSH 180

Query: 181 NTWMIGCFFLLVTSVSWGIWFVLQARFLKEYPHPMEFMCAQTVMSTAQTFVVAIAMERDP 240
             WMIGCFFLLVTS+SWGIWFVLQARFLK YPHPMEFMC QT+MS  Q+F+VAI MERDP
Sbjct: 181 TKWMIGCFFLLVTSISWGIWFVLQARFLKGYPHPMEFMCMQTLMSMVQSFIVAIVMERDP 240

Query: 241 LEWKLGWNVRLYAVLYCGVLVIGVANNAQCWVIREKGPVFQAMTTPLNLMATIIGSLLLL 300
            EWKLG+N+RL+AVLYCG+LVIGVANNAQCWVIREKGPVFQAMTTPLNL+ATIIGS  L 
Sbjct: 241 FEWKLGFNIRLFAVLYCGILVIGVANNAQCWVIREKGPVFQAMTTPLNLIATIIGSQFLF 300

Query: 301 SEGIYVGSIIGASLLVMSLYSVLWGKSKELVTAPNDQQPSPPLKQADESTCRSEVNSTLV 360
            +G Y+GSIIGA LLV SLY VLWGK+KELVT  ND Q SPP K+  ESTC +EVNS+LV
Sbjct: 301 PDGTYLGSIIGAFLLVTSLYCVLWGKNKELVTPSNDGQLSPPQKETKESTCPTEVNSSLV 357

BLAST of CmaCh03G010720 vs. TrEMBL
Match: A0A0A0KJ33_CUCSA (WAT1-related protein OS=Cucumis sativus GN=Csa_6G485670 PE=3 SV=1)

HSP 1 Score: 518.8 bits (1335), Expect = 5.1e-144
Identity = 264/365 (72.33%), Postives = 310/365 (84.93%), Query Frame = 1

Query: 1   MASNNPYIVAFFIQITYAGMSLMSKAAFASGMNTYIFLFYRQAAGTLVLVPLTLLLKGKE 60
           M S  PY+VA FIQIT+AGMSL+SKAAFA+GMNTYIFLFYRQAAG+L+L+PLTLLLKGKE
Sbjct: 1   MDSKKPYLVAIFIQITFAGMSLLSKAAFATGMNTYIFLFYRQAAGSLILIPLTLLLKGKE 60

Query: 61  KRPLSLKHLCQIFVISLIGITLALDAYGVAINYTSATLGAAAFNCVPVTTFFFALILRME 120
           KRPLS K LCQ F ISLIGITLA++AYGVA++YTSATLGAAAFNC+PV+TF FA++ RME
Sbjct: 61  KRPLSFKQLCQCFFISLIGITLAMNAYGVAVDYTSATLGAAAFNCLPVSTFIFAVLFRME 120

Query: 121 KVKLNKAAGIAKVVGIMICMAGAAILAFYKGPYLSPILSHHQLFHHVQTQTQTHHPPTSQ 180
           +V L KAAGIAKV G+MIC+ GAAILAFYKGPYL PI+SH  +FH  +++T      TSQ
Sbjct: 121 RVNLKKAAGIAKVGGMMICVGGAAILAFYKGPYLKPIISH-PIFHIEESETDI--TTTSQ 180

Query: 181 NTWMIGCFFLLVTSVSWGIWFVLQARFLKEYPHPMEFMCAQTVMSTAQTFVVAIAMERDP 240
            +W++GCFFLLV +V WGIWFV QA+FLK YPHP+EFMCAQTVMS  Q FVVAI +ERDP
Sbjct: 181 KSWLLGCFFLLVATVGWGIWFVFQAKFLKGYPHPVEFMCAQTVMSVVQCFVVAIIVERDP 240

Query: 241 LEWKLGWNVRLYAVLYCGVLVIGVANNAQCWVIREKGPVFQAMTTPLNLMATIIGSLLLL 300
            EWKLGWNVRLYAVLYCG+LVIG+ANNAQCWVI+EKGPVFQAM  PLNL+ATIIGS L L
Sbjct: 241 SEWKLGWNVRLYAVLYCGILVIGIANNAQCWVIKEKGPVFQAMMMPLNLVATIIGSQLFL 300

Query: 301 SEGIYVGSIIGASLLVMSLYSVLWGKSKELVTAPNDQQ-----PSPPLKQADESTCRSEV 360
           +EGIY+GS+IGA LLV SLYSVLWGK+KELV  P +Q+      S P K+++E   RS+V
Sbjct: 301 AEGIYLGSVIGAILLVTSLYSVLWGKNKELVVTPTNQERPSSPDSLPQKESEEPANRSQV 360

BLAST of CmaCh03G010720 vs. TrEMBL
Match: A0A0A0KKW0_CUCSA (WAT1-related protein OS=Cucumis sativus GN=Csa_6G485170 PE=3 SV=1)

HSP 1 Score: 474.9 bits (1221), Expect = 8.5e-131
Identity = 245/362 (67.68%), Postives = 290/362 (80.11%), Query Frame = 1

Query: 1   MASNNPYIVAFFIQITYAGMSLMSKAAFASGMNTYIFLFYRQAAGTLVLVPLTLLLKGKE 60
           M S   YIVA F QI YAGMSL SK AFA+GMNTY+FLFYRQAAG+L+L+PLTLLLKGKE
Sbjct: 1   MGSKKFYIVAIFTQIIYAGMSLSSKVAFATGMNTYVFLFYRQAAGSLILIPLTLLLKGKE 60

Query: 61  KRPLSLKHLCQIFVISLIGITLALDAYGVAINYTSATLGAAAFNCVPVTTFFFALILRME 120
           KRPLSLK LC  F ISLIGITL ++AY V I+YTS T GAAA NC+P++TF FA+  RME
Sbjct: 61  KRPLSLKQLCHAFFISLIGITLTMNAYDVGIDYTSPTFGAAAINCIPISTFIFAVTFRME 120

Query: 121 KVKLNKAAGIAKVVGIMICMAGAAILAFYKGPYLSPILSHHQLFHHVQTQTQTHHPPTSQ 180
           KV L KA+GIAKV G+MIC+ GAAILAFYKGPYL PI+SH  +FH  +++T      TSQ
Sbjct: 121 KVNLKKASGIAKVGGMMICVGGAAILAFYKGPYLKPIISH-PIFHFEESETDI--TTTSQ 180

Query: 181 NTWMIGCFFLLVTSVSWGIWFVLQARFLKEYPHPMEFMCAQTVMSTAQTFVVAIAMERDP 240
            +WM+GCF LLV ++ WGIW+V QA FLK+YPHP+E MC QTVMS  Q FVVAI +ERD 
Sbjct: 181 KSWMLGCFILLVATMGWGIWYVFQAMFLKDYPHPVELMCTQTVMSVVQCFVVAIIVERDH 240

Query: 241 LEWKLGWNVRLYAVLYCGVLVIGVANNAQCWVIREKGPVFQAMTTPLNLMATIIGSLLLL 300
            EWKL WNVRLYA+LYCG+LVIG+ANNAQCWVI+E GPVF +M  P+NL+ATIIGS L L
Sbjct: 241 SEWKLDWNVRLYAILYCGILVIGIANNAQCWVIKETGPVFPSMMMPINLVATIIGSQLFL 300

Query: 301 SEGIYVGSIIGASLLVMSLYSVLWGKSKELVTAP-NDQQP-SP---PLKQADESTCRSEV 358
           +EGIY+GS+IGA LLV+SLYSVLWGKSKELV  P N  QP SP   P K+++E   RS+V
Sbjct: 301 AEGIYLGSVIGAILLVISLYSVLWGKSKELVDTPTNQDQPFSPDFLPQKESEEQDSRSQV 359

BLAST of CmaCh03G010720 vs. TrEMBL
Match: A0A0A0KHV4_CUCSA (WAT1-related protein OS=Cucumis sativus GN=Csa_6G485150 PE=3 SV=1)

HSP 1 Score: 385.6 bits (989), Expect = 6.7e-104
Identity = 199/317 (62.78%), Postives = 241/317 (76.03%), Query Frame = 1

Query: 20  MSLMSKAAFASGMNTYIFLFYRQAAGTLVLVPLTLLLKGKEKRPLSLKHLCQIFVISLIG 79
           MSL+SKAAFASGMNT++                      KE R LSLK   +IF+ISLIG
Sbjct: 1   MSLLSKAAFASGMNTFV----------------------KESRSLSLKDFLKIFMISLIG 60

Query: 80  ITLALDAYGVAINYTSATLGAAAFNCVPVTTFFFALILRMEKVKLNKAAGIAKVVGIMIC 139
           +T+  +AYGVA++YTSA LGAAAFNC+PVTTF FA++LRMEKV L K AGIAK +GI+IC
Sbjct: 61  MTIGFNAYGVAVDYTSANLGAAAFNCLPVTTFLFAVLLRMEKVNLRKVAGIAKALGILIC 120

Query: 140 MAGAAILAFYKGPYLSPILSHHQLFHHVQTQTQTHHPPTSQNTWMIGCFFLLVTSVSWGI 199
           + G   LAFYKGPYL P+++HH L  H  +    H   +S  TW+IGCF L ++S+SWG+
Sbjct: 121 IGGVITLAFYKGPYLKPLINHHLLKFHKSSHNIPH--SSSSKTWIIGCFLLFISSISWGL 180

Query: 200 WFVLQARFLKEYPHPMEFMCAQTVMSTAQTFVVAIAMERDPLEWKLGWNVRLYAVLYCGV 259
           WFVLQA FLK YP P+EF+  QT++S AQ+FV+AIAMERDP EWKLGWN+RL AV+YCGV
Sbjct: 181 WFVLQAYFLKTYPSPLEFISYQTLLSAAQSFVIAIAMERDPSEWKLGWNIRLLAVVYCGV 240

Query: 260 LVIGVANNAQCWVIREKGPVFQAMTTPLNLMATIIGSLLLLSEGIYVGSIIGASLLVMSL 319
           LV  V+N  QCWVI+EKGPVFQAMTTPLN++ATIIGS LLL EGI +GS+IGA LLV+SL
Sbjct: 241 LVTVVSNFLQCWVIKEKGPVFQAMTTPLNVIATIIGSELLLGEGINLGSLIGAILLVISL 293

Query: 320 YSVLWGKSKELVTAPND 337
           YSVLWGK+KEL  A  D
Sbjct: 301 YSVLWGKNKELDVADPD 293

BLAST of CmaCh03G010720 vs. TrEMBL
Match: M5X3D1_PRUPE (WAT1-related protein OS=Prunus persica GN=PRUPE_ppa015904mg PE=3 SV=1)

HSP 1 Score: 382.1 bits (980), Expect = 7.5e-103
Identity = 194/360 (53.89%), Postives = 255/360 (70.83%), Query Frame = 1

Query: 1   MASNNPYIVAFFIQITYAGMSLMSKAAFASGMNTYIFLFYRQAAGTLVLVPLTLLLKGKE 60
           M S  PY+   FIQI YAGM L+SKAAF  GMN+Y+F+FYRQ AG+L + PL ++ + K 
Sbjct: 1   MGSTKPYLAVIFIQIIYAGMILLSKAAFNGGMNSYVFVFYRQMAGSLFMSPLAMIFERKS 60

Query: 61  KRPLSLKHLCQIFVISLIGITLALDAYGVAINYTSATLGAAAFNCVPVTTFFFALILRME 120
             PLS+   C+IF++S +GITLA++AY +A+ YTSATLGAA+ NC+PV TFFFA++LRME
Sbjct: 61  ATPLSVVTFCKIFMLSFLGITLAINAYCIALTYTSATLGAASINCLPVATFFFAVLLRME 120

Query: 121 KVKLNKAAGIAKVVGIMICMAGAAILAFYKGPYLSPILSHHQLFHHVQTQTQTH-HPPTS 180
           KVK+  AAGIAKV G+++ M G A+L+FYKGP L P L HH L  H   Q Q   H  +S
Sbjct: 121 KVKIKTAAGIAKVAGLVVSMIGVAVLSFYKGPQLKPFLHHHLLQSHNSHQDQQQAHASSS 180

Query: 181 QNTWMIGCFFLLVTSVSWGIWFVLQARFLKEYPHPMEFMCAQTVMSTAQTFVVAIAMERD 240
            + WMIGCF LLV+ + W +W VLQA+ L +YP  + F   Q + S AQ+FVVAIA+ERD
Sbjct: 181 GHRWMIGCFLLLVSIICWSLWLVLQAQLLVKYPAKLRFTSLQCLSSAAQSFVVAIALERD 240

Query: 241 PLEWKLGWNVRLYAVLYCGVLVIGVANNAQCWVIREKGPVFQAMTTPLNLMATIIGSLLL 300
           P +W LGWN+ L A+ YCG++V GV+   Q +VI +KGPVFQAM+ PLNL+ T+IGS+LL
Sbjct: 241 PSQWNLGWNINLLAIAYCGIMVTGVSYYLQAYVIEKKGPVFQAMSQPLNLIITVIGSVLL 300

Query: 301 LSEGIYVGSIIGASLLVMSLYSVLWGKSKELVTAPNDQQPSPPLKQADESTCRSEVNSTL 360
           L E I +GS++G  L+  SLYSVLWGKSKEL +  N    S P++   E     E  +T+
Sbjct: 301 LGEAISLGSVLGGILMAASLYSVLWGKSKELKSMENRTCLSVPVQPEKERAQLKEAEATI 360

BLAST of CmaCh03G010720 vs. TAIR10
Match: AT5G64700.1 (AT5G64700.1 nodulin MtN21 /EamA-like transporter family protein)

HSP 1 Score: 303.5 bits (776), Expect = 1.7e-82
Identity = 159/340 (46.76%), Postives = 220/340 (64.71%), Query Frame = 1

Query: 1   MASNNPYIVAFFIQITYAGMSLMSKAAFASGMNTYIFLFYRQAAGTLVLVPLTLLLKGKE 60
           M S  PY++   IQ+ Y  M L+SKA F  GMNT++F+FYRQA  T+ L PL    + K 
Sbjct: 3   MESKKPYLMVTIIQVIYTIMFLISKAVFNGGMNTFVFVFYRQAFATIFLAPLAFFFERKS 62

Query: 61  KRPLSLKHLCQIFVISLIGITLALDAYGVAINYTSATLGAAAFNCVPVTTFFFALILRME 120
             PLS     +IF++SL G+TL+LD  G+A++YTSATL AA    +P  TFF AL+  ME
Sbjct: 63  APPLSFVTFIKIFMLSLFGVTLSLDLNGIALSYTSATLAAATTASLPAITFFLALLFGME 122

Query: 121 KVKLNKAAGIAKVVGIMICMAGAAILAFYKGPYLSPILSHHQLFHHVQTQTQTHHP---P 180
           ++K+    G AK+VGI +CM G  ILA YKGP L   L  H  F+H Q     ++P    
Sbjct: 123 RLKVKSIQGTAKLVGITVCMGGVIILAIYKGPLLKLPLCPH--FYHGQEHPHRNNPGHVS 182

Query: 181 TSQNTWMIGCFFLLVTSVSWGIWFVLQARFLKEYPHPMEFMCAQTVMSTAQTFVVAIAME 240
               +W+ GC  ++ +++ WG+W VLQ R LK YP  + F     ++S+ Q+FV+AIA+E
Sbjct: 183 GGSTSWLKGCVLMITSNILWGLWLVLQGRVLKVYPSKLYFTTLHCLLSSIQSFVIAIALE 242

Query: 241 RDPLEWKLGWNVRLYAVLYCGVLVIGVANNAQCWVIREKGPVFQAMTTPLNLMATIIGSL 300
           RD   WKLGWN+RL AV+YCG +V GVA   Q WVI ++GPVF +M TPL+L+ T++ S 
Sbjct: 243 RDISAWKLGWNLRLVAVIYCGFIVTGVAYYLQSWVIEKRGPVFLSMFTPLSLLFTLLSSA 302

Query: 301 LLLSEGIYVGSIIGASLLVMSLYSVLWGKSKELVTAPNDQ 338
           +LL E I +GSI+G  LL++ LY VLWGKS+E   + +D+
Sbjct: 303 ILLCEIISLGSIVGGLLLIIGLYCVLWGKSREEKNSGDDK 340

BLAST of CmaCh03G010720 vs. TAIR10
Match: AT1G43650.1 (AT1G43650.1 nodulin MtN21 /EamA-like transporter family protein)

HSP 1 Score: 238.0 bits (606), Expect = 8.8e-63
Identity = 134/329 (40.73%), Postives = 189/329 (57.45%), Query Frame = 1

Query: 1   MASNNPYIVAFFIQITYAGMSLMSKAAFASGMNTYIFLFYRQAAGTLVLVPLTLLLKGKE 60
           M  +   +   F+QI YAGM L+SK A + G N ++F+FYRQA   L L P    L+  +
Sbjct: 2   MMEHKANMAMVFVQIVYAGMPLLSKVAISQGTNPFVFVFYRQAFAALALSPFAFFLESSK 61

Query: 61  KRPLSLKHLCQIFVISLIGITLALDAYGVAINYTSATLGAAAFNCVPVTTFFFALILRME 120
             PLS   L +IF ISL G+TL+L+ Y VAI  T+AT  AA  N +P  TF  AL+ R+E
Sbjct: 62  SSPLSFILLLKIFFISLCGLTLSLNLYYVAIENTTATFAAATTNAIPSITFVLALLFRLE 121

Query: 121 KVKLNKAAGIAKVVGIMICMAGAAILAFYKGPYLSPILSHHQLFHHVQTQTQTHHPPTSQ 180
            V L K+ G+AKV G M+ M GA + AF KGP          L +H  + T  +    S 
Sbjct: 122 TVTLKKSHGVAKVTGSMVGMLGALVFAFVKGP---------SLINHYNSSTIPNGTVPST 181

Query: 181 NTWMIGCFFLLVTSVSWGIWFVLQARFLKEYPHPMEFMCAQTVMSTAQTFVVAIAMERDP 240
              + G   +L  +  W +W ++Q++ +KEYP  +  +  Q + S  Q+ V A+A+ R+P
Sbjct: 182 KNSVKGSITMLAANTCWCLWIIMQSKVMKEYPAKLRLVALQCLFSCIQSAVWAVAVNRNP 241

Query: 241 LEWKLGWNVRLYAVLYCGVLVIGVANNAQCWVIREKGPVFQAMTTPLNLMATIIGSLLLL 300
             WK+ + + L ++ YCG++V G+    Q W I +KGPVF A+ TPL L+ T I S  L 
Sbjct: 242 SVWKIEFGLPLLSMAYCGIMVTGLTYWLQVWAIEKKGPVFTALYTPLALILTCIVSSFLF 301

Query: 301 SEGIYVGSIIGASLLVMSLYSVLWGKSKE 330
            E  Y+GS+ GA LLV  LY  LWGK+KE
Sbjct: 302 KETFYLGSVGGAVLLVCGLYLGLWGKTKE 321

BLAST of CmaCh03G010720 vs. TAIR10
Match: AT1G75500.1 (AT1G75500.1 Walls Are Thin 1)

HSP 1 Score: 196.1 bits (497), Expect = 3.8e-50
Identity = 114/326 (34.97%), Postives = 173/326 (53.07%), Query Frame = 1

Query: 7   YIVAFFIQITYAGMSLMSKAAFASGMNTYIFLFYRQAAGTLVLVPLTLLLKGKEKRPLSL 66
           +I    +Q  YAG  ++S+AA   G++  +F  YR     L+L+P    L+ KE+  ++L
Sbjct: 21  HIAMLTLQFGYAGFHVVSRAALNMGISKLVFPVYRNIIALLLLLPFAYFLEKKERPAITL 80

Query: 67  KHLCQIFVISLIGITLALDAYGVAINYTSATLGAAAFNCVPVTTFFFALILRMEKVKLNK 126
             L Q F ++LIGIT     Y + ++ TS T  ++  N VP  TF  A +LR+EKV++N+
Sbjct: 81  NFLIQFFFLALIGITANQGFYLLGLDNTSPTFASSMQNSVPAITFLMAALLRIEKVRINR 140

Query: 127 AAGIAKVVGIMICMAGAAILAFYKGPYLSPILSHHQLFHHVQTQTQTHHPP---TSQNTW 186
             GI+K++G  +C+AGA+++  YKGP +    SH  L  H+ T       P    +   W
Sbjct: 141 RDGISKILGTALCVAGASVITLYKGPTIYTPASH--LHAHLLTTNSAVLAPLGNAAPKNW 200

Query: 187 MIGCFFLLVTSVSWGIWFVLQARFLKEYPHPMEFMCAQTVMSTAQTFVVAIAMERDPLEW 246
            +GC +L+   +SW  W V QA  LK YP  +            Q  ++A   ERD   W
Sbjct: 201 TLGCIYLIGHCLSWSGWLVFQAPVLKSYPARLSVTSYTCFFGIIQFLIIAAFCERDSQAW 260

Query: 247 KLGWNVRLYAVLYCGVLVIGVANNAQCWVIREKGPVFQAMTTPLNLMATIIGSLLLLSEG 306
                  L+ +LY G++  G+A   Q W I   GPVF A+  P+  +   I + + L E 
Sbjct: 261 VFHSGWELFTILYAGIVASGIAFAVQIWCIDRGGPVFVAVYQPVQTLVVAIMASIALGEE 320

Query: 307 IYVGSIIGASLLVMSLYSVLWGKSKE 330
            Y+G IIGA L++  LY VL+GKS+E
Sbjct: 321 FYLGGIIGAVLIIAGLYFVLYGKSEE 344

BLAST of CmaCh03G010720 vs. TAIR10
Match: AT5G07050.1 (AT5G07050.1 nodulin MtN21 /EamA-like transporter family protein)

HSP 1 Score: 189.9 bits (481), Expect = 2.8e-48
Identity = 104/334 (31.14%), Postives = 179/334 (53.59%), Query Frame = 1

Query: 1   MASNNPYIVAFFIQITYAGMSLMSKAAFASGMNTYIFLFYRQAAGTLVLVPLTLLLKGKE 60
           + S+ PY     +Q  YAGM++++K +  +GM+ Y+ + YR A  T V+ P     + K 
Sbjct: 13  LTSSKPYFAMISLQFGYAGMNIITKISLNTGMSHYVLVVYRHAIATAVIAPFAFFFERKA 72

Query: 61  KRPLSLKHLCQIFVISLIGITLALDAYGVAINYTSATLGAAAFNCVPVTTFFFALILRME 120
           +  ++     Q+F++ L+G  +  + Y + + YTS T   A  N +P  TF  A++ RME
Sbjct: 73  QPKITFSIFMQLFILGLLGPVIDQNFYYMGLKYTSPTFSCAMSNMLPAMTFILAVLFRME 132

Query: 121 KVKLNKAAGIAKVVGIMICMAGAAILAFYKGPYLSPILSHHQLFHHVQ----TQTQTHHP 180
            + L K    AK+ G ++ +AGA ++  YKGP +    + +    H+Q      T +   
Sbjct: 133 MLDLKKLWCQAKIAGTVVTVAGAMLMTIYKGPIVELFWTKYM---HIQDSSHANTTSSKN 192

Query: 181 PTSQNTWMIGCFFLLVTSVSWGIWFVLQARFLKEY-PHPMEFMCAQTVMSTAQTFVVAIA 240
            +S   ++ G   L+  +++W   FVLQA+ LK Y  H +        + T Q   V   
Sbjct: 193 SSSDKEFLKGSILLIFATLAWASLFVLQAKILKTYAKHQLSLTTLICFIGTLQAVAVTFV 252

Query: 241 MERDPLEWKLGWNVRLYAVLYCGVLVIGVANNAQCWVIREKGPVFQAMTTPLNLMATIIG 300
           ME +P  W++GW++ L A  Y G++   ++   Q  V++++GPVF    +PL ++   + 
Sbjct: 253 MEHNPSAWRIGWDMNLLAAAYSGIVASSISYYVQGIVMKKRGPVFATAFSPLMMVIVAVM 312

Query: 301 SLLLLSEGIYVGSIIGASLLVMSLYSVLWGKSKE 330
              +L+E I++G +IGA L+V+ LY+VLWGK KE
Sbjct: 313 GSFVLAEKIFLGGVIGAVLIVIGLYAVLWGKQKE 343

BLAST of CmaCh03G010720 vs. TAIR10
Match: AT1G44800.1 (AT1G44800.1 nodulin MtN21 /EamA-like transporter family protein)

HSP 1 Score: 188.3 bits (477), Expect = 8.0e-48
Identity = 110/338 (32.54%), Postives = 185/338 (54.73%), Query Frame = 1

Query: 1   MASNNPYIVAFFIQITYAGMSLMSKAAFASGMNTYIFLFYRQAAGTLVLVPLTLLLKGKE 60
           M    P +    +Q  YAGM +++  +F  GM+ ++   YR    T+V+ P  L+ + K 
Sbjct: 6   MEKIKPILAIISLQFGYAGMYIITMVSFKHGMDHWVLATYRHVVATVVMAPFALMFERKI 65

Query: 61  KRPLSLKHLCQIFVISLIGITLALDAYGVAINYTSATLGAAAFNCVPVTTFFFALILRME 120
           +  ++L    ++  + ++   +  + Y + +  TSA+  +A  N +P  TF  ALI R+E
Sbjct: 66  RPKMTLAIFWRLLALGILEPLMDQNLYYIGLKNTSASYTSAFTNALPAVTFILALIFRLE 125

Query: 121 KVKLNKAAGIAKVVGIMICMAGAAILAFYKGPYLSPILSHHQLFHHVQTQTQTHHPPTSQ 180
            V   K   +AKVVG +I + GA I+  YKGP +  + + H  FH   + T     PT Q
Sbjct: 126 TVNFRKVHSVAKVVGTVITVGGAMIMTLYKGPAIEIVKAAHNSFHGGSSST-----PTGQ 185

Query: 181 NTWMIGCFFLLVTSVSWGIWFVLQARFLKEYPHPMEFMCAQTVMSTAQTFVVAIAMERDP 240
           + W++G   ++ +  +W  +F+LQ+  LK YP  +  +     + T    + ++ M RDP
Sbjct: 186 H-WVLGTIAIMGSISTWAAFFILQSYTLKVYPAELSLVTLICGIGTILNAIASLIMVRDP 245

Query: 241 LEWKLGWNVRLYAVLYCGVLVIGVANNAQCWVIREKGPVFQAMTTPLNLMATIIGSLLLL 300
             WK+G +    A +Y GV+  G+A   Q  VI+++GPVF    +P+ ++ T     L+L
Sbjct: 246 SAWKIGMDSGTLAAVYSGVVCSGIAYYIQSIVIKQRGPVFTTSFSPMCMIITAFLGALVL 305

Query: 301 SEGIYVGSIIGASLLVMSLYSVLWGKSKELVTAPNDQQ 339
           +E I++GSIIGA  +V+ LYSV+WGKSK+ V  P D++
Sbjct: 306 AEKIHLGSIIGAVFIVLGLYSVVWGKSKDEVN-PLDEK 336

BLAST of CmaCh03G010720 vs. NCBI nr
Match: gi|659081074|ref|XP_008441136.1| (PREDICTED: WAT1-related protein At5g64700-like [Cucumis melo])

HSP 1 Score: 572.4 bits (1474), Expect = 5.6e-160
Identity = 291/360 (80.83%), Postives = 322/360 (89.44%), Query Frame = 1

Query: 1   MASNNPYIVAFFIQITYAGMSLMSKAAFASGMNTYIFLFYRQAAGTLVLVPLTLLLKGKE 60
           M SN PYIVA FIQIT+AGMSLMSKAAFA+GMNTYIFLFYRQAAG+++LVPLTL+LKGKE
Sbjct: 68  MGSNKPYIVAVFIQITFAGMSLMSKAAFAAGMNTYIFLFYRQAAGSIILVPLTLILKGKE 127

Query: 61  KRPLSLKHLCQIFVISLIGITLALDAYGVAINYTSATLGAAAFNCVPVTTFFFALILRME 120
           KRPL+LKHLCQIFVISLIGITLALDAYGVAINYTSAT+GAAAFNCVPVTTFFFA++LRME
Sbjct: 128 KRPLTLKHLCQIFVISLIGITLALDAYGVAINYTSATIGAAAFNCVPVTTFFFAVLLRME 187

Query: 121 KVKLNKAAGIAKVVGIMICMAGAAILAFYKGPYLSPILSHHQLFHHVQTQTQTHHPPTSQ 180
           KV L KAAGIAKVVGIMIC+AGA+ILA YKGPYL P+ +H QLFHH+ +Q+  HH   S 
Sbjct: 188 KVNLKKAAGIAKVVGIMICIAGASILALYKGPYLKPLFTH-QLFHHIHSQS--HHSLNSH 247

Query: 181 NTWMIGCFFLLVTSVSWGIWFVLQARFLKEYPHPMEFMCAQTVMSTAQTFVVAIAMERDP 240
           N WMIGCFFLLVTSVSWGIWFVLQARFLK YPHPMEFMC QT+MS  Q+F+VAI MERDP
Sbjct: 248 NKWMIGCFFLLVTSVSWGIWFVLQARFLKGYPHPMEFMCMQTLMSMVQSFIVAIVMERDP 307

Query: 241 LEWKLGWNVRLYAVLYCGVLVIGVANNAQCWVIREKGPVFQAMTTPLNLMATIIGSLLLL 300
           LEWKLG+N+RL+AVLYCG+LVIG+ANNAQCWVIREKGPVFQAMTTPLNL+ATIIGS  L 
Sbjct: 308 LEWKLGFNIRLFAVLYCGILVIGIANNAQCWVIREKGPVFQAMTTPLNLIATIIGSQFLF 367

Query: 301 SEGIYVGSIIGASLLVMSLYSVLWGKSKELVTAPNDQQPSPPLKQADESTCRSEVNSTLV 360
            +G Y+GSIIGA LLV SLY VLWGKSKELVT  ND Q SPP K+ D+ST R EVNS+LV
Sbjct: 368 PDGTYLGSIIGAFLLVTSLYCVLWGKSKELVTPSNDVQLSPPQKETDQSTRRPEVNSSLV 424

BLAST of CmaCh03G010720 vs. NCBI nr
Match: gi|778717886|ref|XP_011657775.1| (PREDICTED: WAT1-related protein At5g64700-like isoform X1 [Cucumis sativus])

HSP 1 Score: 568.5 bits (1464), Expect = 8.1e-159
Identity = 288/360 (80.00%), Postives = 318/360 (88.33%), Query Frame = 1

Query: 1   MASNNPYIVAFFIQITYAGMSLMSKAAFASGMNTYIFLFYRQAAGTLVLVPLTLLLKGKE 60
           M SN PYIV FFIQIT+AGMSLMSKAAFA+GMNTYIFLFYRQAAG+++LVPLTL+LKGKE
Sbjct: 1   MGSNKPYIVVFFIQITFAGMSLMSKAAFAAGMNTYIFLFYRQAAGSIILVPLTLILKGKE 60

Query: 61  KRPLSLKHLCQIFVISLIGITLALDAYGVAINYTSATLGAAAFNCVPVTTFFFALILRME 120
           KRPLSLKHLCQIFVISLIGITLALDAYGVAINYTSAT+GAAAFNCVPVTTFFFA++LRME
Sbjct: 61  KRPLSLKHLCQIFVISLIGITLALDAYGVAINYTSATIGAAAFNCVPVTTFFFAVLLRME 120

Query: 121 KVKLNKAAGIAKVVGIMICMAGAAILAFYKGPYLSPILSHHQLFHHVQTQTQTHHPPTSQ 180
           KV L KAAGIAKVVGIMIC+ GA+ILA YKGPYL P+ +H QLFHH+ +Q   HH   S 
Sbjct: 121 KVNLKKAAGIAKVVGIMICITGASILALYKGPYLKPLFTH-QLFHHIHSQP--HHSLNSH 180

Query: 181 NTWMIGCFFLLVTSVSWGIWFVLQARFLKEYPHPMEFMCAQTVMSTAQTFVVAIAMERDP 240
             WMIGCFFLLVTS+SWGIWFVLQARFLK YPHPMEFMC QT+MS  Q+F+VAI MERDP
Sbjct: 181 TKWMIGCFFLLVTSISWGIWFVLQARFLKGYPHPMEFMCMQTLMSMVQSFIVAIVMERDP 240

Query: 241 LEWKLGWNVRLYAVLYCGVLVIGVANNAQCWVIREKGPVFQAMTTPLNLMATIIGSLLLL 300
            EWKLG+N+RL+AVLYCG+LVIGVANNAQCWVIREKGPVFQAMTTPLNL+ATIIGS  L 
Sbjct: 241 FEWKLGFNIRLFAVLYCGILVIGVANNAQCWVIREKGPVFQAMTTPLNLIATIIGSQFLF 300

Query: 301 SEGIYVGSIIGASLLVMSLYSVLWGKSKELVTAPNDQQPSPPLKQADESTCRSEVNSTLV 360
            +G Y+GSIIGA LLV SLY VLWGK+KELVT  ND Q SPP K+  ESTC +EVNS+LV
Sbjct: 301 PDGTYLGSIIGAFLLVTSLYCVLWGKNKELVTPSNDGQLSPPQKETKESTCPTEVNSSLV 357

BLAST of CmaCh03G010720 vs. NCBI nr
Match: gi|659080822|ref|XP_008440998.1| (PREDICTED: WAT1-related protein At5g64700-like [Cucumis melo])

HSP 1 Score: 525.8 bits (1353), Expect = 6.0e-146
Identity = 271/365 (74.25%), Postives = 311/365 (85.21%), Query Frame = 1

Query: 1   MASNNPYIVAFFIQITYAGMSLMSKAAFASGMNTYIFLFYRQAAGTLVLVPLTLLLKGKE 60
           M +  PYIVA FIQIT+AGMSL+SKAAFA+GMNTYIFLFYRQAAG+L+LVPLT LLKGKE
Sbjct: 1   MDAKKPYIVAIFIQITFAGMSLLSKAAFATGMNTYIFLFYRQAAGSLILVPLTFLLKGKE 60

Query: 61  KRPLSLKHLCQIFVISLIGITLALDAYGVAINYTSATLGAAAFNCVPVTTFFFALILRME 120
           KRPLSLK LC +F ISLIGITLA++AYGVA++YTSATLGAAAFNC+PV+TF FA++ RME
Sbjct: 61  KRPLSLKQLCHVFFISLIGITLAMNAYGVAVDYTSATLGAAAFNCLPVSTFIFAVLFRME 120

Query: 121 KVKLNKAAGIAKVVGIMICMAGAAILAFYKGPYLSPILSHHQLFHHVQTQTQTHHPPTSQ 180
           KV L KAAGIAKV G+MIC+ GAAILAFYKGPYL PI+SH  LFH  +++T      TSQ
Sbjct: 121 KVNLKKAAGIAKVAGMMICVGGAAILAFYKGPYLKPIISH-PLFHIEKSETDI--TATSQ 180

Query: 181 NTWMIGCFFLLVTSVSWGIWFVLQARFLKEYPHPMEFMCAQTVMSTAQTFVVAIAMERDP 240
            +W++GCFFLLV +V WGIWFVLQA+FLK YPHP+EFMCAQTVMS  Q FVVAI +ERDP
Sbjct: 181 KSWLLGCFFLLVATVGWGIWFVLQAKFLKGYPHPVEFMCAQTVMSVVQCFVVAIIVERDP 240

Query: 241 LEWKLGWNVRLYAVLYCGVLVIGVANNAQCWVIREKGPVFQAMTTPLNLMATIIGSLLLL 300
            EWKLGWNVRLYAVLYCG+LVIG+ANNAQCWVI+EKGPVFQAM  PLNL+ATIIGS L L
Sbjct: 241 SEWKLGWNVRLYAVLYCGILVIGIANNAQCWVIKEKGPVFQAMMMPLNLVATIIGSQLFL 300

Query: 301 SEGIYVGSIIGASLLVMSLYSVLWGKSKELVTAPNDQQP--SP---PLKQADESTCRSEV 360
           +EGIY+GS+IGA LLV SLYSVLWGKSKELV  P +Q P  SP   P K+  E T RS+V
Sbjct: 301 AEGIYLGSVIGAILLVTSLYSVLWGKSKELVVTPTNQDPPSSPDPLPQKEPKEPTNRSQV 360

BLAST of CmaCh03G010720 vs. NCBI nr
Match: gi|449448590|ref|XP_004142049.1| (PREDICTED: WAT1-related protein At5g64700-like [Cucumis sativus])

HSP 1 Score: 518.8 bits (1335), Expect = 7.3e-144
Identity = 264/365 (72.33%), Postives = 310/365 (84.93%), Query Frame = 1

Query: 1   MASNNPYIVAFFIQITYAGMSLMSKAAFASGMNTYIFLFYRQAAGTLVLVPLTLLLKGKE 60
           M S  PY+VA FIQIT+AGMSL+SKAAFA+GMNTYIFLFYRQAAG+L+L+PLTLLLKGKE
Sbjct: 1   MDSKKPYLVAIFIQITFAGMSLLSKAAFATGMNTYIFLFYRQAAGSLILIPLTLLLKGKE 60

Query: 61  KRPLSLKHLCQIFVISLIGITLALDAYGVAINYTSATLGAAAFNCVPVTTFFFALILRME 120
           KRPLS K LCQ F ISLIGITLA++AYGVA++YTSATLGAAAFNC+PV+TF FA++ RME
Sbjct: 61  KRPLSFKQLCQCFFISLIGITLAMNAYGVAVDYTSATLGAAAFNCLPVSTFIFAVLFRME 120

Query: 121 KVKLNKAAGIAKVVGIMICMAGAAILAFYKGPYLSPILSHHQLFHHVQTQTQTHHPPTSQ 180
           +V L KAAGIAKV G+MIC+ GAAILAFYKGPYL PI+SH  +FH  +++T      TSQ
Sbjct: 121 RVNLKKAAGIAKVGGMMICVGGAAILAFYKGPYLKPIISH-PIFHIEESETDI--TTTSQ 180

Query: 181 NTWMIGCFFLLVTSVSWGIWFVLQARFLKEYPHPMEFMCAQTVMSTAQTFVVAIAMERDP 240
            +W++GCFFLLV +V WGIWFV QA+FLK YPHP+EFMCAQTVMS  Q FVVAI +ERDP
Sbjct: 181 KSWLLGCFFLLVATVGWGIWFVFQAKFLKGYPHPVEFMCAQTVMSVVQCFVVAIIVERDP 240

Query: 241 LEWKLGWNVRLYAVLYCGVLVIGVANNAQCWVIREKGPVFQAMTTPLNLMATIIGSLLLL 300
            EWKLGWNVRLYAVLYCG+LVIG+ANNAQCWVI+EKGPVFQAM  PLNL+ATIIGS L L
Sbjct: 241 SEWKLGWNVRLYAVLYCGILVIGIANNAQCWVIKEKGPVFQAMMMPLNLVATIIGSQLFL 300

Query: 301 SEGIYVGSIIGASLLVMSLYSVLWGKSKELVTAPNDQQ-----PSPPLKQADESTCRSEV 360
           +EGIY+GS+IGA LLV SLYSVLWGK+KELV  P +Q+      S P K+++E   RS+V
Sbjct: 301 AEGIYLGSVIGAILLVTSLYSVLWGKNKELVVTPTNQERPSSPDSLPQKESEEPANRSQV 360

BLAST of CmaCh03G010720 vs. NCBI nr
Match: gi|778717898|ref|XP_011657778.1| (PREDICTED: WAT1-related protein At5g64700-like isoform X1 [Cucumis sativus])

HSP 1 Score: 474.9 bits (1221), Expect = 1.2e-130
Identity = 245/362 (67.68%), Postives = 290/362 (80.11%), Query Frame = 1

Query: 1   MASNNPYIVAFFIQITYAGMSLMSKAAFASGMNTYIFLFYRQAAGTLVLVPLTLLLKGKE 60
           M S   YIVA F QI YAGMSL SK AFA+GMNTY+FLFYRQAAG+L+L+PLTLLLKGKE
Sbjct: 1   MGSKKFYIVAIFTQIIYAGMSLSSKVAFATGMNTYVFLFYRQAAGSLILIPLTLLLKGKE 60

Query: 61  KRPLSLKHLCQIFVISLIGITLALDAYGVAINYTSATLGAAAFNCVPVTTFFFALILRME 120
           KRPLSLK LC  F ISLIGITL ++AY V I+YTS T GAAA NC+P++TF FA+  RME
Sbjct: 61  KRPLSLKQLCHAFFISLIGITLTMNAYDVGIDYTSPTFGAAAINCIPISTFIFAVTFRME 120

Query: 121 KVKLNKAAGIAKVVGIMICMAGAAILAFYKGPYLSPILSHHQLFHHVQTQTQTHHPPTSQ 180
           KV L KA+GIAKV G+MIC+ GAAILAFYKGPYL PI+SH  +FH  +++T      TSQ
Sbjct: 121 KVNLKKASGIAKVGGMMICVGGAAILAFYKGPYLKPIISH-PIFHFEESETDI--TTTSQ 180

Query: 181 NTWMIGCFFLLVTSVSWGIWFVLQARFLKEYPHPMEFMCAQTVMSTAQTFVVAIAMERDP 240
            +WM+GCF LLV ++ WGIW+V QA FLK+YPHP+E MC QTVMS  Q FVVAI +ERD 
Sbjct: 181 KSWMLGCFILLVATMGWGIWYVFQAMFLKDYPHPVELMCTQTVMSVVQCFVVAIIVERDH 240

Query: 241 LEWKLGWNVRLYAVLYCGVLVIGVANNAQCWVIREKGPVFQAMTTPLNLMATIIGSLLLL 300
            EWKL WNVRLYA+LYCG+LVIG+ANNAQCWVI+E GPVF +M  P+NL+ATIIGS L L
Sbjct: 241 SEWKLDWNVRLYAILYCGILVIGIANNAQCWVIKETGPVFPSMMMPINLVATIIGSQLFL 300

Query: 301 SEGIYVGSIIGASLLVMSLYSVLWGKSKELVTAP-NDQQP-SP---PLKQADESTCRSEV 358
           +EGIY+GS+IGA LLV+SLYSVLWGKSKELV  P N  QP SP   P K+++E   RS+V
Sbjct: 301 AEGIYLGSVIGAILLVISLYSVLWGKSKELVDTPTNQDQPFSPDFLPQKESEEQDSRSQV 359

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
WTR45_ARATH3.0e-8146.76WAT1-related protein At5g64700 OS=Arabidopsis thaliana GN=At5g64700 PE=2 SV=1[more]
WTR7_ARATH1.6e-6140.73WAT1-related protein At1g43650 OS=Arabidopsis thaliana GN=At1g43650 PE=2 SV=1[more]
WAT1_ARATH6.8e-4934.97Protein WALLS ARE THIN 1 OS=Arabidopsis thaliana GN=WAT1 PE=1 SV=1[more]
WTR38_ARATH4.9e-4731.14WAT1-related protein At5g07050 OS=Arabidopsis thaliana GN=At5g07050 PE=2 SV=1[more]
WTR8_ARATH1.4e-4632.54WAT1-related protein At1g44800 OS=Arabidopsis thaliana GN=At1g44800 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0KFA3_CUCSA5.6e-15980.00WAT1-related protein OS=Cucumis sativus GN=Csa_6G485160 PE=3 SV=1[more]
A0A0A0KJ33_CUCSA5.1e-14472.33WAT1-related protein OS=Cucumis sativus GN=Csa_6G485670 PE=3 SV=1[more]
A0A0A0KKW0_CUCSA8.5e-13167.68WAT1-related protein OS=Cucumis sativus GN=Csa_6G485170 PE=3 SV=1[more]
A0A0A0KHV4_CUCSA6.7e-10462.78WAT1-related protein OS=Cucumis sativus GN=Csa_6G485150 PE=3 SV=1[more]
M5X3D1_PRUPE7.5e-10353.89WAT1-related protein OS=Prunus persica GN=PRUPE_ppa015904mg PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT5G64700.11.7e-8246.76 nodulin MtN21 /EamA-like transporter family protein[more]
AT1G43650.18.8e-6340.73 nodulin MtN21 /EamA-like transporter family protein[more]
AT1G75500.13.8e-5034.97 Walls Are Thin 1[more]
AT5G07050.12.8e-4831.14 nodulin MtN21 /EamA-like transporter family protein[more]
AT1G44800.18.0e-4832.54 nodulin MtN21 /EamA-like transporter family protein[more]
Match NameE-valueIdentityDescription
gi|659081074|ref|XP_008441136.1|5.6e-16080.83PREDICTED: WAT1-related protein At5g64700-like [Cucumis melo][more]
gi|778717886|ref|XP_011657775.1|8.1e-15980.00PREDICTED: WAT1-related protein At5g64700-like isoform X1 [Cucumis sativus][more]
gi|659080822|ref|XP_008440998.1|6.0e-14674.25PREDICTED: WAT1-related protein At5g64700-like [Cucumis melo][more]
gi|449448590|ref|XP_004142049.1|7.3e-14472.33PREDICTED: WAT1-related protein At5g64700-like [Cucumis sativus][more]
gi|778717898|ref|XP_011657778.1|1.2e-13067.68PREDICTED: WAT1-related protein At5g64700-like isoform X1 [Cucumis sativus][more]
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR000620EamA_dom
Vocabulary: Cellular Component
TermDefinition
GO:0016021integral component of membrane
GO:0016020membrane
Vocabulary: Molecular Function
TermDefinition
GO:0022857transmembrane transporter activity
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0055085 transmembrane transport
biological_process GO:0008150 biological_process
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0016020 membrane
molecular_function GO:0022857 transmembrane transporter activity

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh03G010720.1CmaCh03G010720.1mRNA


Analysis Name: InterPro Annotations of Cucurbita maxima
Date Performed: 2017-05-20
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000620EamA domainPFAMPF00892EamAcoord: 6..134
score: 1.1E-10coord: 184..323
score: 2.4
NoneNo IPR availablePANTHERPTHR31218:SF15SUBFAMILY NOT NAMEDcoord: 1..329
score: 4.0E
NoneNo IPR availableunknownSSF103481Multidrug resistance efflux transporter EmrEcoord: 40..149
score: 8.

The following gene(s) are paralogous to this gene:
GeneParalogueOrganismBlock
CmaCh03G010720CmaCh02G005840Cucurbita maxima (Rimu)cmacmaB499
CmaCh03G010720CmaCh07G007800Cucurbita maxima (Rimu)cmacmaB523