CmaCh16G008230 (gene) Cucurbita maxima (Rimu)

NameCmaCh16G008230
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu))
DescriptionSesquiterpene synthase
LocationCma_Chr16 : 4577683 .. 4578570 (+)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAAGCGGCGAACGGTGCATGTAGAAGATGTCCTTGAGGAAGCTCTTGATTTTAGTGTCAAGCATCTAAAAAATATTATAGCCAACACAAACTCCAAATTTGGTGCTGAAATTGTACCGTGCCTTGAAATAGCCACCAAGAAAAGACCTTCCAAGATTAAAAGCAAGAGAGTACATATCAATGTATCAAGAAAATCCTTCCCATTCTCAAACTTTGCTTGCTTTTCAAAACTGGATTTTAACATATTACAAAAATTTCATCAAAAGGAGCGGAAAGAACTCACAAGGTGCAATTTCATATATTTTTTTCTCTAATAATTTTATTTAGCTTTTCCAACTTCATATTCGTTAAACTATTGATATAATTAAATTTTAAATAACCTAACCTATCATATTAATTGAGTTTAATAATAATTTAAAATGATATTAAAAAATGACTTAGAACTAAATTATTGTCCCTTCTTCTCCGATTAATGTTTGGACTTTCATAAATTTGTGTGAGAAGGTTAAAATATTGATTTAATATTTTTGGGTTCATTGGTAATATTTTATTTTAAATTATAGGCTCTTTCTCTTCTATAAATTATATTTCCTTCTTATTTACGTATTTTATATTTTTATAGGTGGTGGAAAGATTTGAACGTCCAAACAAATTTTCCTTATGCCAGAGATAAAATTGTTGAGTGTTACTTTTGGACATTGGGTGCATACTTTGAACCTCGGTTTAATGTTGGAAGGAAAATATTAACCAAAGTAATCGTCATTTCTTCCATTTTAGATGACACATATGATACCTATGGAACATTTGAAGAACTCAAAATCTTCCCTCAAACAATTCAAAGATATTTGACAAAGATCTTAGCCTTAAAAAGCTTGACTTACAACTAA

mRNA sequence

ATGAAGCGGCGAACGGTGCATGTAGAAGATGTCCTTGAGGAAGCTCTTGATTTTAGTGTCAAGCATCTAAAAAATATTATAGCCAACACAAACTCCAAATTTGGTGCTGAAATTGTACCGTGCCTTGAAATAGCCACCAAGAAAAGACCTTCCAAGATTAAAAGCAAGAGAGTACATATCAATGTATCAAGAAAATCCTTCCCATTCTCAAACTTTGCTTGCTTTTCAAAACTGGATTTTAACATATTACAAAAATTTCATCAAAAGGAGCGGAAAGAACTCACAAGGTGGTGGAAAGATTTGAACGTCCAAACAAATTTTCCTTATGCCAGAGATAAAATTGTTGAGTGTTACTTTTGGACATTGGGTGCATACTTTGAACCTCGGTTTAATGTTGGAAGGAAAATATTAACCAAAGTAATCGTCATTTCTTCCATTTTAGATGACACATATGATACCTATGGAACATTTGAAGAACTCAAAATCTTCCCTCAAACAATTCAAAGATATTTGACAAAGATCTTAGCCTTAAAAAGCTTGACTTACAACTAA

Coding sequence (CDS)

ATGAAGCGGCGAACGGTGCATGTAGAAGATGTCCTTGAGGAAGCTCTTGATTTTAGTGTCAAGCATCTAAAAAATATTATAGCCAACACAAACTCCAAATTTGGTGCTGAAATTGTACCGTGCCTTGAAATAGCCACCAAGAAAAGACCTTCCAAGATTAAAAGCAAGAGAGTACATATCAATGTATCAAGAAAATCCTTCCCATTCTCAAACTTTGCTTGCTTTTCAAAACTGGATTTTAACATATTACAAAAATTTCATCAAAAGGAGCGGAAAGAACTCACAAGGTGGTGGAAAGATTTGAACGTCCAAACAAATTTTCCTTATGCCAGAGATAAAATTGTTGAGTGTTACTTTTGGACATTGGGTGCATACTTTGAACCTCGGTTTAATGTTGGAAGGAAAATATTAACCAAAGTAATCGTCATTTCTTCCATTTTAGATGACACATATGATACCTATGGAACATTTGAAGAACTCAAAATCTTCCCTCAAACAATTCAAAGATATTTGACAAAGATCTTAGCCTTAAAAAGCTTGACTTACAACTAA

Protein sequence

MKRRTVHVEDVLEEALDFSVKHLKNIIANTNSKFGAEIVPCLEIATKKRPSKIKSKRVHINVSRKSFPFSNFACFSKLDFNILQKFHQKERKELTRWWKDLNVQTNFPYARDKIVECYFWTLGAYFEPRFNVGRKILTKVIVISSILDDTYDTYGTFEELKIFPQTIQRYLTKILALKSLTYN
BLAST of CmaCh16G008230 vs. Swiss-Prot
Match: GCSY1_CUCME ((+)-gamma-cadinene synthase OS=Cucumis melo PE=1 SV=1)

HSP 1 Score: 183.0 bits (463), Expect = 3.0e-45
Identity = 90/167 (53.89%), Postives = 116/167 (69.46%), Query Frame = 1

Query: 7   HVEDVLEEALDFSVKHLKNII---ANTNSKFGAEIVPCLEIATKKRPSKIKSKRVHINVS 66
           H E +LEEAL+F+  HL+  I   +N N  F +E+   L++  +K   +IK++       
Sbjct: 179 HGEALLEEALEFTTTHLQTYIHRYSNINPSFASEVSNALKLPIRKSVPRIKAREYLEIYQ 238

Query: 67  RKSFPFSNFACFSKLDFNILQKFHQKERKELTRWWKDLNVQTNFPYARDKIVECYFWTLG 126
           +          FSKLDFNILQK HQKE  E+ RWWKDL+V T FP+ARD+IVECYFWTLG
Sbjct: 239 QHPSHNETLLEFSKLDFNILQKLHQKELSEICRWWKDLDVPTKFPFARDRIVECYFWTLG 298

Query: 127 AYFEPRFNVGRKILTKVIVISSILDDTYDTYGTFEELKIFPQTIQRY 171
           AYFEP+++VGRK+LTKVI I+SILDD YD YGTFEEL++    IQR+
Sbjct: 299 AYFEPQYSVGRKMLTKVIAIASILDDIYDAYGTFEELQVLTPAIQRW 345

BLAST of CmaCh16G008230 vs. Swiss-Prot
Match: TPSGB_SOLHA ((E,E)-germacrene B synthase OS=Solanum habrochaites GN=SSTLH1 PE=1 SV=1)

HSP 1 Score: 157.9 bits (398), Expect = 1.0e-37
Identity = 75/169 (44.38%), Postives = 116/169 (68.64%), Query Frame = 1

Query: 6   VHVEDVLEEALDFSVKHLKNIIA---NTNSKFGAEIVPCLEIATKKRPSKIKSKRVHINV 65
           V  E++LEEAL F+  HLK+I++   N N+    E+   L    +    +++++R +I++
Sbjct: 151 VRDEEILEEALAFTTTHLKSIVSTMSNNNNSLKVEVSEALTQPIRMTLPRMEARR-YISI 210

Query: 66  SRKSFPFSNFAC-FSKLDFNILQKFHQKERKELTRWWKDLNVQTNFPYARDKIVECYFWT 125
              +   ++    F+KLDFN+LQK HQ+E  +LTRWWKDL+    +PYARD++VECYFW 
Sbjct: 211 YENNDAHNHLLLKFAKLDFNMLQKLHQRELSDLTRWWKDLDFANKYPYARDRLVECYFWI 270

Query: 126 LGAYFEPRFNVGRKILTKVIVISSILDDTYDTYGTFEELKIFPQTIQRY 171
           LG YFEP+++  RK++TKV+ ++SI+DDT+D Y TF+EL+ F   IQR+
Sbjct: 271 LGVYFEPKYSRARKMMTKVLKMTSIIDDTFDAYATFDELEPFNDAIQRW 318

BLAST of CmaCh16G008230 vs. Swiss-Prot
Match: TPS2_VALOF (Valerena-4,7(11)-diene synthase OS=Valeriana officinalis GN=TPS2 PE=1 SV=1)

HSP 1 Score: 156.0 bits (393), Expect = 4.0e-37
Identity = 70/167 (41.92%), Postives = 111/167 (66.47%), Query Frame = 1

Query: 6   VHVEDVLEEALDFSVKHLKNII--ANTNSKFGAEIVPCLEIATKKRPSKIKSKRVHINVS 65
           VH ED+LEEAL+F++  L+ ++  ++ ++   +E++  L +  +K  ++I+++       
Sbjct: 170 VHKEDILEEALEFTIYELEQVVKLSSNDTLLASEVIHALNMPIRKGLTRIEARHFISVYQ 229

Query: 66  RKSFPFSNFACFSKLDFNILQKFHQKERKELTRWWKDLNVQTNFPYARDKIVECYFWTLG 125
                      FSK+DFN+LQK HQ+E  +LT WW+ LNV    PYARD+ VECYFW LG
Sbjct: 230 HDKSHDETLLKFSKIDFNMLQKLHQRELADLTIWWEKLNVAEKMPYARDRFVECYFWGLG 289

Query: 126 AYFEPRFNVGRKILTKVIVISSILDDTYDTYGTFEELKIFPQTIQRY 171
            YFEP+++  RK+  KVI ++S++DDTYD+YGTF+EL +F   ++R+
Sbjct: 290 VYFEPQYSRARKMFVKVINLTSLIDDTYDSYGTFDELDLFTDAVKRW 336

BLAST of CmaCh16G008230 vs. Swiss-Prot
Match: TPSGC_SOLLC (Germacrene C synthase OS=Solanum lycopersicum GN=SSTLE1 PE=1 SV=1)

HSP 1 Score: 155.2 bits (391), Expect = 6.8e-37
Identity = 74/169 (43.79%), Postives = 114/169 (67.46%), Query Frame = 1

Query: 6   VHVEDVLEEALDFSVKHLKNIIAN---TNSKFGAEIVPCLEIATKKRPSKIKSKRVHINV 65
           V  E++LEEAL F+  HL++I++N    N+    E+   L    +    ++ +++ +I++
Sbjct: 155 VRNEEILEEALTFTTTHLESIVSNLSNNNNSLKVEVGEALTQPIRMTLPRMGARK-YISI 214

Query: 66  SRKSFPFSNFAC-FSKLDFNILQKFHQKERKELTRWWKDLNVQTNFPYARDKIVECYFWT 125
              +    +    F+KLDFN+LQKFHQ+E  +LTRWWKDL+    +PYARD++VECYFW 
Sbjct: 215 YENNDAHHHLLLKFAKLDFNMLQKFHQRELSDLTRWWKDLDFANKYPYARDRLVECYFWI 274

Query: 126 LGAYFEPRFNVGRKILTKVIVISSILDDTYDTYGTFEELKIFPQTIQRY 171
           LG YFEP+++  RK++TKV+ ++SI+DDT+D Y TF+EL  F   IQR+
Sbjct: 275 LGVYFEPKYSRARKMMTKVLNLTSIIDDTFDAYATFDELVTFNDAIQRW 322

BLAST of CmaCh16G008230 vs. Swiss-Prot
Match: TPS1_VALOF (Germacrene C/D synthase OS=Valeriana officinalis GN=TPS1 PE=1 SV=1)

HSP 1 Score: 154.5 bits (389), Expect = 1.2e-36
Identity = 75/167 (44.91%), Postives = 109/167 (65.27%), Query Frame = 1

Query: 6   VHVEDVLEEALDFSVKHLKNIIANTNSK--FGAEIVPCLEIATKKRPSKIKSKRVHINVS 65
           VH ED+LEEAL+F+V  L+ +I ++ S     +++V  L I  +K  ++++++       
Sbjct: 171 VHKEDILEEALEFTVAQLEQVIKSSLSDKVLLSQVVHALNIPIRKSLTRLEARYFISVYE 230

Query: 66  RKSFPFSNFACFSKLDFNILQKFHQKERKELTRWWKDLNVQTNFPYARDKIVECYFWTLG 125
           +          FSKLDFNILQK HQ+E  +LT WWK+LNV    PYARD++VECYFW L 
Sbjct: 231 QDKSCNETLLKFSKLDFNILQKLHQQEVADLTLWWKNLNVSEKVPYARDRLVECYFWALA 290

Query: 126 AYFEPRFNVGRKILTKVIVISSILDDTYDTYGTFEELKIFPQTIQRY 171
            YFEP+++  RK+  K+  + S++DDTYD+YGTFEEL +F    QR+
Sbjct: 291 EYFEPQYSRARKMSGKITALISLIDDTYDSYGTFEELALFTDAAQRW 337

BLAST of CmaCh16G008230 vs. TrEMBL
Match: A0A0A0L2I8_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G097540 PE=3 SV=1)

HSP 1 Score: 187.6 bits (475), Expect = 1.4e-44
Identity = 92/167 (55.09%), Postives = 117/167 (70.06%), Query Frame = 1

Query: 7   HVEDVLEEALDFSVKHLK---NIIANTNSKFGAEIVPCLEIATKKRPSKIKSKRVHINVS 66
           H E +LEEAL+F+  HLK   ++ +N N  F +E+   L++  +K   +IK++       
Sbjct: 166 HGEALLEEALEFTTTHLKAYIHLYSNINPNFASEVSNALKLPIRKCVPRIKAREYFQIYQ 225

Query: 67  RKSFPFSNFACFSKLDFNILQKFHQKERKELTRWWKDLNVQTNFPYARDKIVECYFWTLG 126
           +          FSKLDFNILQK HQKE  E+ RWWKDL+V T FP+ARD+IVECYFWTLG
Sbjct: 226 QHPSHSETLLEFSKLDFNILQKLHQKELSEICRWWKDLDVPTKFPFARDRIVECYFWTLG 285

Query: 127 AYFEPRFNVGRKILTKVIVISSILDDTYDTYGTFEELKIFPQTIQRY 171
           AYFEP++NVGRK+LTKVI ISSILDD YD YGTFEEL++    IQR+
Sbjct: 286 AYFEPQYNVGRKMLTKVIAISSILDDIYDAYGTFEELQVLAPAIQRW 332

BLAST of CmaCh16G008230 vs. TrEMBL
Match: Q66PX8_CUCSA (Beta-caryophyllene synthase OS=Cucumis sativus PE=2 SV=1)

HSP 1 Score: 179.9 bits (455), Expect = 2.9e-42
Identity = 87/167 (52.10%), Postives = 114/167 (68.26%), Query Frame = 1

Query: 7   HVEDVLEEALDFSVKHLK---NIIANTNSKFGAEIVPCLEIATKKRPSKIKSKRVHINVS 66
           H E +LEEAL+F+  HLK   ++ +N N  F +E+   L++  +K   ++K++       
Sbjct: 176 HGEALLEEALEFTTTHLKAYIHLYSNINPNFASEVSNALKLPIRKCVPRVKAREYFEIYQ 235

Query: 67  RKSFPFSNFACFSKLDFNILQKFHQKERKELTRWWKDLNVQTNFPYARDKIVECYFWTLG 126
           ++         FSKLDFNILQK HQKE  E+ RWWKDLNV TNFP+ARD+IVECYFW L 
Sbjct: 236 QQPSHNETLLTFSKLDFNILQKLHQKEIAEICRWWKDLNVSTNFPFARDRIVECYFWILS 295

Query: 127 AYFEPRFNVGRKILTKVIVISSILDDTYDTYGTFEELKIFPQTIQRY 171
            YFEP F  GRKILTKVI ++SI+DD YD YGTFEEL++F   I+R+
Sbjct: 296 IYFEPYFKFGRKILTKVIAMTSIMDDIYDAYGTFEELQLFTLAIKRW 342

BLAST of CmaCh16G008230 vs. TrEMBL
Match: A0A0A0L579_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G097040 PE=3 SV=1)

HSP 1 Score: 179.9 bits (455), Expect = 2.9e-42
Identity = 87/167 (52.10%), Postives = 114/167 (68.26%), Query Frame = 1

Query: 7   HVEDVLEEALDFSVKHLK---NIIANTNSKFGAEIVPCLEIATKKRPSKIKSKRVHINVS 66
           H E +LEEAL+F+  HLK   ++ +N N  F +E+   L++  +K   ++K++       
Sbjct: 176 HGEALLEEALEFTTTHLKAYIHLYSNINPNFASEVSNALKLPIRKCVPRVKAREYFEIYQ 235

Query: 67  RKSFPFSNFACFSKLDFNILQKFHQKERKELTRWWKDLNVQTNFPYARDKIVECYFWTLG 126
           ++         FSKLDFNILQK HQKE  E+ RWWKDLNV TNFP+ARD+IVECYFW L 
Sbjct: 236 QQPSHNETLLTFSKLDFNILQKLHQKEIAEICRWWKDLNVSTNFPFARDRIVECYFWILS 295

Query: 127 AYFEPRFNVGRKILTKVIVISSILDDTYDTYGTFEELKIFPQTIQRY 171
            YFEP F  GRKILTKVI ++SI+DD YD YGTFEEL++F   I+R+
Sbjct: 296 IYFEPYFKFGRKILTKVIAMTSIMDDIYDAYGTFEELQLFTLAIKRW 342

BLAST of CmaCh16G008230 vs. TrEMBL
Match: A0A0B4VEV4_CUCME (Sesquiterpene synthase Tps1 OS=Cucumis melo PE=2 SV=1)

HSP 1 Score: 179.1 bits (453), Expect = 4.9e-42
Identity = 87/168 (51.79%), Postives = 113/168 (67.26%), Query Frame = 1

Query: 6   VHVEDVLEEALDFSVKHLKNII---ANTNSKFGAEIVPCLEIATKKRPSKIKSKRVHINV 65
           VH E +LEEAL+F+  HL+  +   +N N  F +E+   L++  +K   ++K++      
Sbjct: 178 VHGEALLEEALEFTTTHLQTYVHRYSNINPSFASEVSNALKLPIRKGVPRMKAREYLEIY 237

Query: 66  SRKSFPFSNFACFSKLDFNILQKFHQKERKELTRWWKDLNVQTNFPYARDKIVECYFWTL 125
            +          FSKLDFN LQK HQKE  E+ RWWKDLNV TNFP+ARD+IVECYFW L
Sbjct: 238 QQHPSHNETLLTFSKLDFNALQKLHQKELAEICRWWKDLNVSTNFPFARDQIVECYFWIL 297

Query: 126 GAYFEPRFNVGRKILTKVIVISSILDDTYDTYGTFEELKIFPQTIQRY 171
             YFEPRF  GRKILTKVI ++SI+DD YD YGTFEEL++F   I+R+
Sbjct: 298 SIYFEPRFKFGRKILTKVIAMTSIMDDIYDAYGTFEELQVFTLAIKRW 345

BLAST of CmaCh16G008230 vs. TrEMBL
Match: T2D1F3_9ROSI (Sesquiterpene synthase OS=Aquilaria sinensis PE=2 SV=1)

HSP 1 Score: 163.7 bits (413), Expect = 2.1e-37
Identity = 82/170 (48.24%), Postives = 114/170 (67.06%), Query Frame = 1

Query: 6   VHVEDVLEEALDFSVKHLKNIIANTNS--KFGAEIVPCLEIATKKRPSKIKSKRVHINVS 65
           +H ED+LE+A DFS KHLK+I  +T+S  K  A+    L+    K   ++++K  +I+V 
Sbjct: 152 LHGEDILEQAFDFSTKHLKSIAEDTSSSPKLAAQASQALKYPVPKNIPRLEAK-YYISV- 211

Query: 66  RKSFPFSN---FACFSKLDFNILQKFHQKERKELTRWWKDLNVQTNFPYARDKIVECYFW 125
              +P  N      F++LDFNILQK HQ E +EL RWWKDL++    PYARD+I E +FW
Sbjct: 212 ---YPLLNTPVVLAFAELDFNILQKLHQNELRELARWWKDLDIPRRLPYARDRITELFFW 271

Query: 126 TLGAYFEPRFNVGRKILTKVIVISSILDDTYDTYGTFEELKIFPQTIQRY 171
            L  Y+EP + +GRKILTKVI ++S+LDD YD YGT EEL++  + IQR+
Sbjct: 272 VLAVYYEPCYTLGRKILTKVIALTSLLDDMYDAYGTIEELELLTKAIQRW 316

BLAST of CmaCh16G008230 vs. TAIR10
Match: AT5G23960.1 (AT5G23960.1 terpene synthase 21)

HSP 1 Score: 115.2 bits (287), Expect = 4.4e-26
Identity = 56/165 (33.94%), Postives = 93/165 (56.36%), Query Frame = 1

Query: 7   HVEDVLEEALDFSVKHLKNIIANTNSKFGAEIVPCLEIATKKRPSKIKSKRVHINVSRKS 66
           H ED+++EAL FS  HL+ I + ++      I   L+    K  S+I++++       + 
Sbjct: 158 HGEDIIDEALAFSRSHLEEISSRSSPHLAIRIKNALKHPYHKGISRIETRQYISYYEEEE 217

Query: 67  FPFSNFACFSKLDFNILQKFHQKERKELTRWWKDLNVQTNFPYARDKIVECYFWTLGAYF 126
                   F+K+DFN+LQ  H++E   +TRW  ++  ++   Y R +I E Y W+LG YF
Sbjct: 218 SCDPTLLEFAKIDFNLLQILHREELACVTRWHHEMEFKSKVTYTRHRITEAYLWSLGTYF 277

Query: 127 EPRFNVGRKILTKVIVISSILDDTYDTYGTFEELKIFPQTIQRYL 172
           EP+++  R I T  +++ + LDD YD YGT EEL++F   +  +L
Sbjct: 278 EPQYSQARVITTMALILFTALDDMYDAYGTMEELELFTDAMDEWL 322

BLAST of CmaCh16G008230 vs. TAIR10
Match: AT3G14490.1 (AT3G14490.1 Terpenoid cyclases/Protein prenyltransferases superfamily protein)

HSP 1 Score: 109.4 bits (272), Expect = 2.4e-24
Identity = 59/170 (34.71%), Postives = 96/170 (56.47%), Query Frame = 1

Query: 9   EDVLEEALDFSVKHLKNIIANTNSKFGAEIVPCLEIATKKRPSKIKSKRVHINVSRKSFP 68
           ED+++EAL F+   L+++ +N      A   P L    +    + +   + I V+R+   
Sbjct: 211 EDIMDEALSFTRYRLESLTSNHT----ATASPHLSKHIQNALYRARYHNLEILVAREYIS 270

Query: 69  F--------SNFACFSKLDFNILQKFHQKERKELTRWWKDLNVQTNFPYARDKIVECYFW 128
           F             F+KL+FN  Q  + +E K+LT+WWK+L++ +  PY RD+IVE YF 
Sbjct: 271 FYEQEEDHDETLLKFAKLNFNYCQLHYIQELKDLTKWWKELDLASKLPYIRDRIVEVYFG 330

Query: 129 TLGAYFEPRFNVGRKILTKVIVISSILDDTYDTYGTFEELKIFPQTIQRY 171
            L  YFEPR+++GR I+TK+ +I ++ +DT D YGT  E+     + QR+
Sbjct: 331 ALALYFEPRYSLGRIIVTKITMIVTVFNDTCDAYGTLPEVTSLVDSFQRW 376

BLAST of CmaCh16G008230 vs. TAIR10
Match: AT4G16730.1 (AT4G16730.1 terpene synthase 02)

HSP 1 Score: 107.8 bits (268), Expect = 7.0e-24
Identity = 47/115 (40.87%), Postives = 71/115 (61.74%), Query Frame = 1

Query: 57  RVHINVSRKSFPFSNFAC-FSKLDFNILQKFHQKERKELTRWWKDLNVQTNFPYARDKIV 116
           R +INV  K    +     F+ +DFN+LQ  HQ+E K ++ WW    +     + RD+I 
Sbjct: 197 RWYINVYEKKHEMNPLLLEFAAIDFNMLQANHQEELKLISSWWNSTGLMKQLDFVRDRIT 256

Query: 117 ECYFWTLGAYFEPRFNVGRKILTKVIVISSILDDTYDTYGTFEELKIFPQTIQRY 171
           E YFWT+G ++EP F   RKILTK+ ++  I+DD YD YGT EEL++F   ++++
Sbjct: 257 ESYFWTIGIFYEPEFKYCRKILTKIFMLIVIMDDIYDIYGTLEELELFTNVVEKW 311

BLAST of CmaCh16G008230 vs. TAIR10
Match: AT3G25830.1 (AT3G25830.1 terpene synthase-like sequence-1,8-cineole)

HSP 1 Score: 107.5 bits (267), Expect = 9.1e-24
Identity = 44/96 (45.83%), Postives = 67/96 (69.79%), Query Frame = 1

Query: 75  FSKLDFNILQKFHQKERKELTRWWKDLNVQTNFPYARDKIVECYFWTLGAYFEPRFNVGR 134
           F+K+DFNI+Q  HQ++ K ++RWWKD  +    P+ RD+IVE YFWT+G  +EP+F   R
Sbjct: 269 FAKIDFNIVQAAHQEDLKYVSRWWKDTCLTNQLPFVRDRIVENYFWTVGLIYEPQFGYIR 328

Query: 135 KILTKVIVISSILDDTYDTYGTFEELKIFPQTIQRY 171
           +I+T V  + + +DD YD YGT EEL++F   ++ +
Sbjct: 329 RIMTIVNALVTTIDDIYDIYGTLEELELFTSMVENW 364

BLAST of CmaCh16G008230 vs. TAIR10
Match: AT3G25820.1 (AT3G25820.1 terpene synthase-like sequence-1,8-cineole)

HSP 1 Score: 107.5 bits (267), Expect = 9.1e-24
Identity = 44/96 (45.83%), Postives = 67/96 (69.79%), Query Frame = 1

Query: 75  FSKLDFNILQKFHQKERKELTRWWKDLNVQTNFPYARDKIVECYFWTLGAYFEPRFNVGR 134
           F+K+DFNI+Q  HQ++ K ++RWWKD  +    P+ RD+IVE YFWT+G  +EP+F   R
Sbjct: 269 FAKIDFNIVQAAHQEDLKYVSRWWKDTCLTNQLPFVRDRIVENYFWTVGLIYEPQFGYIR 328

Query: 135 KILTKVIVISSILDDTYDTYGTFEELKIFPQTIQRY 171
           +I+T V  + + +DD YD YGT EEL++F   ++ +
Sbjct: 329 RIMTIVNALVTTIDDIYDIYGTLEELELFTSMVENW 364

BLAST of CmaCh16G008230 vs. NCBI nr
Match: gi|778676208|ref|XP_011650545.1| (PREDICTED: (+)-gamma-cadinene synthase [Cucumis sativus])

HSP 1 Score: 187.6 bits (475), Expect = 2.0e-44
Identity = 92/167 (55.09%), Postives = 117/167 (70.06%), Query Frame = 1

Query: 7   HVEDVLEEALDFSVKHLK---NIIANTNSKFGAEIVPCLEIATKKRPSKIKSKRVHINVS 66
           H E +LEEAL+F+  HLK   ++ +N N  F +E+   L++  +K   +IK++       
Sbjct: 166 HGEALLEEALEFTTTHLKAYIHLYSNINPNFASEVSNALKLPIRKCVPRIKAREYFQIYQ 225

Query: 67  RKSFPFSNFACFSKLDFNILQKFHQKERKELTRWWKDLNVQTNFPYARDKIVECYFWTLG 126
           +          FSKLDFNILQK HQKE  E+ RWWKDL+V T FP+ARD+IVECYFWTLG
Sbjct: 226 QHPSHSETLLEFSKLDFNILQKLHQKELSEICRWWKDLDVPTKFPFARDRIVECYFWTLG 285

Query: 127 AYFEPRFNVGRKILTKVIVISSILDDTYDTYGTFEELKIFPQTIQRY 171
           AYFEP++NVGRK+LTKVI ISSILDD YD YGTFEEL++    IQR+
Sbjct: 286 AYFEPQYNVGRKMLTKVIAISSILDDIYDAYGTFEELQVLAPAIQRW 332

BLAST of CmaCh16G008230 vs. NCBI nr
Match: gi|661902982|ref|NP_001284382.1| ((+)-gamma-cadinene synthase [Cucumis melo])

HSP 1 Score: 183.0 bits (463), Expect = 4.8e-43
Identity = 90/167 (53.89%), Postives = 116/167 (69.46%), Query Frame = 1

Query: 7   HVEDVLEEALDFSVKHLKNII---ANTNSKFGAEIVPCLEIATKKRPSKIKSKRVHINVS 66
           H E +LEEAL+F+  HL+  I   +N N  F +E+   L++  +K   +IK++       
Sbjct: 179 HGEALLEEALEFTTTHLQTYIHRYSNINPSFASEVSNALKLPIRKSVPRIKAREYLEIYQ 238

Query: 67  RKSFPFSNFACFSKLDFNILQKFHQKERKELTRWWKDLNVQTNFPYARDKIVECYFWTLG 126
           +          FSKLDFNILQK HQKE  E+ RWWKDL+V T FP+ARD+IVECYFWTLG
Sbjct: 239 QHPSHNETLLEFSKLDFNILQKLHQKELSEICRWWKDLDVPTKFPFARDRIVECYFWTLG 298

Query: 127 AYFEPRFNVGRKILTKVIVISSILDDTYDTYGTFEELKIFPQTIQRY 171
           AYFEP+++VGRK+LTKVI I+SILDD YD YGTFEEL++    IQR+
Sbjct: 299 AYFEPQYSVGRKMLTKVIAIASILDDIYDAYGTFEELQVLTPAIQRW 345

BLAST of CmaCh16G008230 vs. NCBI nr
Match: gi|700201056|gb|KGN56189.1| (hypothetical protein Csa_3G097040 [Cucumis sativus])

HSP 1 Score: 179.9 bits (455), Expect = 4.1e-42
Identity = 87/167 (52.10%), Postives = 114/167 (68.26%), Query Frame = 1

Query: 7   HVEDVLEEALDFSVKHLK---NIIANTNSKFGAEIVPCLEIATKKRPSKIKSKRVHINVS 66
           H E +LEEAL+F+  HLK   ++ +N N  F +E+   L++  +K   ++K++       
Sbjct: 176 HGEALLEEALEFTTTHLKAYIHLYSNINPNFASEVSNALKLPIRKCVPRVKAREYFEIYQ 235

Query: 67  RKSFPFSNFACFSKLDFNILQKFHQKERKELTRWWKDLNVQTNFPYARDKIVECYFWTLG 126
           ++         FSKLDFNILQK HQKE  E+ RWWKDLNV TNFP+ARD+IVECYFW L 
Sbjct: 236 QQPSHNETLLTFSKLDFNILQKLHQKEIAEICRWWKDLNVSTNFPFARDRIVECYFWILS 295

Query: 127 AYFEPRFNVGRKILTKVIVISSILDDTYDTYGTFEELKIFPQTIQRY 171
            YFEP F  GRKILTKVI ++SI+DD YD YGTFEEL++F   I+R+
Sbjct: 296 IYFEPYFKFGRKILTKVIAMTSIMDDIYDAYGTFEELQLFTLAIKRW 342

BLAST of CmaCh16G008230 vs. NCBI nr
Match: gi|793420197|ref|NP_001292628.1| ((+)-gamma-cadinene synthase [Cucumis sativus])

HSP 1 Score: 179.9 bits (455), Expect = 4.1e-42
Identity = 87/167 (52.10%), Postives = 114/167 (68.26%), Query Frame = 1

Query: 7   HVEDVLEEALDFSVKHLK---NIIANTNSKFGAEIVPCLEIATKKRPSKIKSKRVHINVS 66
           H E +LEEAL+F+  HLK   ++ +N N  F +E+   L++  +K   ++K++       
Sbjct: 176 HGEALLEEALEFTTTHLKAYIHLYSNINPNFASEVSNALKLPIRKCVPRVKAREYFEIYQ 235

Query: 67  RKSFPFSNFACFSKLDFNILQKFHQKERKELTRWWKDLNVQTNFPYARDKIVECYFWTLG 126
           ++         FSKLDFNILQK HQKE  E+ RWWKDLNV TNFP+ARD+IVECYFW L 
Sbjct: 236 QQPSHNETLLTFSKLDFNILQKLHQKEIAEICRWWKDLNVSTNFPFARDRIVECYFWILS 295

Query: 127 AYFEPRFNVGRKILTKVIVISSILDDTYDTYGTFEELKIFPQTIQRY 171
            YFEP F  GRKILTKVI ++SI+DD YD YGTFEEL++F   I+R+
Sbjct: 296 IYFEPYFKFGRKILTKVIAMTSIMDDIYDAYGTFEELQLFTLAIKRW 342

BLAST of CmaCh16G008230 vs. NCBI nr
Match: gi|745672488|gb|AJD19682.1| (sesquiterpene synthase Tps1 [Cucumis melo])

HSP 1 Score: 179.1 bits (453), Expect = 7.0e-42
Identity = 87/168 (51.79%), Postives = 113/168 (67.26%), Query Frame = 1

Query: 6   VHVEDVLEEALDFSVKHLKNII---ANTNSKFGAEIVPCLEIATKKRPSKIKSKRVHINV 65
           VH E +LEEAL+F+  HL+  +   +N N  F +E+   L++  +K   ++K++      
Sbjct: 178 VHGEALLEEALEFTTTHLQTYVHRYSNINPSFASEVSNALKLPIRKGVPRMKAREYLEIY 237

Query: 66  SRKSFPFSNFACFSKLDFNILQKFHQKERKELTRWWKDLNVQTNFPYARDKIVECYFWTL 125
            +          FSKLDFN LQK HQKE  E+ RWWKDLNV TNFP+ARD+IVECYFW L
Sbjct: 238 QQHPSHNETLLTFSKLDFNALQKLHQKELAEICRWWKDLNVSTNFPFARDQIVECYFWIL 297

Query: 126 GAYFEPRFNVGRKILTKVIVISSILDDTYDTYGTFEELKIFPQTIQRY 171
             YFEPRF  GRKILTKVI ++SI+DD YD YGTFEEL++F   I+R+
Sbjct: 298 SIYFEPRFKFGRKILTKVIAMTSIMDDIYDAYGTFEELQVFTLAIKRW 345

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
GCSY1_CUCME3.0e-4553.89(+)-gamma-cadinene synthase OS=Cucumis melo PE=1 SV=1[more]
TPSGB_SOLHA1.0e-3744.38(E,E)-germacrene B synthase OS=Solanum habrochaites GN=SSTLH1 PE=1 SV=1[more]
TPS2_VALOF4.0e-3741.92Valerena-4,7(11)-diene synthase OS=Valeriana officinalis GN=TPS2 PE=1 SV=1[more]
TPSGC_SOLLC6.8e-3743.79Germacrene C synthase OS=Solanum lycopersicum GN=SSTLE1 PE=1 SV=1[more]
TPS1_VALOF1.2e-3644.91Germacrene C/D synthase OS=Valeriana officinalis GN=TPS1 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0L2I8_CUCSA1.4e-4455.09Uncharacterized protein OS=Cucumis sativus GN=Csa_3G097540 PE=3 SV=1[more]
Q66PX8_CUCSA2.9e-4252.10Beta-caryophyllene synthase OS=Cucumis sativus PE=2 SV=1[more]
A0A0A0L579_CUCSA2.9e-4252.10Uncharacterized protein OS=Cucumis sativus GN=Csa_3G097040 PE=3 SV=1[more]
A0A0B4VEV4_CUCME4.9e-4251.79Sesquiterpene synthase Tps1 OS=Cucumis melo PE=2 SV=1[more]
T2D1F3_9ROSI2.1e-3748.24Sesquiterpene synthase OS=Aquilaria sinensis PE=2 SV=1[more]
Match NameE-valueIdentityDescription
AT5G23960.14.4e-2633.94 terpene synthase 21[more]
AT3G14490.12.4e-2434.71 Terpenoid cyclases/Protein prenyltransferases superfamily protein[more]
AT4G16730.17.0e-2440.87 terpene synthase 02[more]
AT3G25830.19.1e-2445.83 terpene synthase-like sequence-1,8-cineole[more]
AT3G25820.19.1e-2445.83 terpene synthase-like sequence-1,8-cineole[more]
Match NameE-valueIdentityDescription
gi|778676208|ref|XP_011650545.1|2.0e-4455.09PREDICTED: (+)-gamma-cadinene synthase [Cucumis sativus][more]
gi|661902982|ref|NP_001284382.1|4.8e-4353.89(+)-gamma-cadinene synthase [Cucumis melo][more]
gi|700201056|gb|KGN56189.1|4.1e-4252.10hypothetical protein Csa_3G097040 [Cucumis sativus][more]
gi|793420197|ref|NP_001292628.1|4.1e-4252.10(+)-gamma-cadinene synthase [Cucumis sativus][more]
gi|745672488|gb|AJD19682.1|7.0e-4251.79sesquiterpene synthase Tps1 [Cucumis melo][more]
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR001906Terpene_synth_N
IPR005630Terpene_synthase_metal-bd
IPR008949Isoprenoid_synthase_dom_sf
Vocabulary: Biological Process
TermDefinition
GO:0008152metabolic process
Vocabulary: Molecular Function
TermDefinition
GO:0010333terpene synthase activity
GO:0016829lyase activity
GO:0000287magnesium ion binding
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0045338 farnesyl diphosphate metabolic process
biological_process GO:0051762 sesquiterpene biosynthetic process
biological_process GO:0008152 metabolic process
cellular_component GO:0005575 cellular_component
molecular_function GO:0000287 magnesium ion binding
molecular_function GO:0010334 sesquiterpene synthase activity
molecular_function GO:0016829 lyase activity
molecular_function GO:0010333 terpene synthase activity

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh16G008230.1CmaCh16G008230.1mRNA


Analysis Name: InterPro Annotations of Cucurbita maxima
Date Performed: 2017-05-20
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001906Terpene synthase, N-terminal domainGENE3DG3DSA:1.50.30.10coord: 6..76
score: 1.
IPR005630Terpene synthase, metal-binding domainPFAMPF03936Terpene_synth_Ccoord: 75..172
score: 8.7
IPR008949Isoprenoid synthase domainGENE3DG3DSA:1.10.600.10coord: 77..170
score: 4.0
IPR008949Isoprenoid synthase domainunknownSSF48576Terpenoid synthasescoord: 75..170
score: 2.76
NoneNo IPR availablePANTHERPTHR31225FAMILY NOT NAMEDcoord: 6..170
score: 7.5
NoneNo IPR availablePANTHERPTHR31225:SF15ALPHA-HUMULENE/(-)-(E)-BETA-CARYOPHYLLENE SYNTHASEcoord: 6..170
score: 7.5

The following gene(s) are orthologous to this gene:
GeneOrthologueOrganismBlock
CmaCh16G008230CmoCh16G008820Cucurbita moschata (Rifu)cmacmoB322
CmaCh16G008230Cp4.1LG00g00300Cucurbita pepo (Zucchini)cmacpeB337
CmaCh16G008230MELO3C016593.2Melon (DHL92) v3.6.1cmamedB345
CmaCh16G008230Bhi11G000588Wax gourdcmawgoB0417
The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:
GeneOrganismBlock
CmaCh16G008230Watermelon (97103) v1cmawmB306
CmaCh16G008230Cucurbita pepo (Zucchini)cmacpeB350
CmaCh16G008230Cucurbita pepo (Zucchini)cmacpeB356
CmaCh16G008230Cucurbita pepo (Zucchini)cmacpeB362
CmaCh16G008230Bottle gourd (USVL1VR-Ls)cmalsiB315
CmaCh16G008230Cucumber (Gy14) v2cgybcmaB084
CmaCh16G008230Cucumber (Gy14) v2cgybcmaB341
CmaCh16G008230Melon (DHL92) v3.6.1cmamedB330
CmaCh16G008230Silver-seed gourdcarcmaB0946
CmaCh16G008230Silver-seed gourdcarcmaB1243
CmaCh16G008230Cucumber (Chinese Long) v3cmacucB0371
CmaCh16G008230Cucumber (Chinese Long) v3cmacucB0382
CmaCh16G008230Watermelon (97103) v2cmawmbB336
CmaCh16G008230Watermelon (97103) v2cmawmbB343
CmaCh16G008230Wax gourdcmawgoB0421
CmaCh16G008230Cucurbita maxima (Rimu)cmacmaB041
CmaCh16G008230Cucurbita maxima (Rimu)cmacmaB339
CmaCh16G008230Cucurbita maxima (Rimu)cmacmaB350
CmaCh16G008230Cucumber (Gy14) v1cgycmaB0121
CmaCh16G008230Cucumber (Gy14) v1cgycmaB0337
CmaCh16G008230Cucurbita moschata (Rifu)cmacmoB317
CmaCh16G008230Cucurbita moschata (Rifu)cmacmoB324
CmaCh16G008230Cucurbita moschata (Rifu)cmacmoB328
CmaCh16G008230Cucurbita moschata (Rifu)cmacmoB337
CmaCh16G008230Wild cucumber (PI 183967)cmacpiB324
CmaCh16G008230Wild cucumber (PI 183967)cmacpiB334
CmaCh16G008230Cucumber (Chinese Long) v2cmacuB318
CmaCh16G008230Cucumber (Chinese Long) v2cmacuB328
CmaCh16G008230Melon (DHL92) v3.5.1cmameB292
CmaCh16G008230Watermelon (Charleston Gray)cmawcgB283
CmaCh16G008230Watermelon (Charleston Gray)cmawcgB294
CmaCh16G008230Watermelon (Charleston Gray)cmawcgB298