CmaCh16G008230.1 (mRNA) Cucurbita maxima (Rimu) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	CmaCh16G008230.1
Type	mRNA
Organism	Cucurbita maxima (Cucurbita maxima (Rimu))
Description	Sesquiterpene synthase
Location	Cma_Chr16 : 4577683 .. 4578570 (+)
Sequence length	552

Genome Viewer

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAAGCGGCGAACGGTGCATGTAGAAGATGTCCTTGAGGAAGCTCTTGATTTTAGTGTCAAGCATCTAAAAAATATTATAGCCAACACAAACTCCAAATTTGGTGCTGAAATTGTACCGTGCCTTGAAATAGCCACCAAGAAAAGACCTTCCAAGATTAAAAGCAAGAGAGTACATATCAATGTATCAAGAAAATCCTTCCCATTCTCAAACTTTGCTTGCTTTTCAAAACTGGATTTTAACATATTACAAAAATTTCATCAAAAGGAGCGGAAAGAACTCACAAGGTGCAATTTCATATATTTTTTTCTCTAATAATTTTATTTAGCTTTTCCAACTTCATATTCGTTAAACTATTGATATAATTAAATTTTAAATAACCTAACCTATCATATTAATTGAGTTTAATAATAATTTAAAATGATATTAAAAAATGACTTAGAACTAAATTATTGTCCCTTCTTCTCCGATTAATGTTTGGACTTTCATAAATTTGTGTGAGAAGGTTAAAATATTGATTTAATATTTTTGGGTTCATTGGTAATATTTTATTTTAAATTATAGGCTCTTTCTCTTCTATAAATTATATTTCCTTCTTATTTACGTATTTTATATTTTTATAGGTGGTGGAAAGATTTGAACGTCCAAACAAATTTTCCTTATGCCAGAGATAAAATTGTTGAGTGTTACTTTTGGACATTGGGTGCATACTTTGAACCTCGGTTTAATGTTGGAAGGAAAATATTAACCAAAGTAATCGTCATTTCTTCCATTTTAGATGACACATATGATACCTATGGAACATTTGAAGAACTCAAAATCTTCCCTCAAACAATTCAAAGATATTTGACAAAGATCTTAGCCTTAAAAAGCTTGACTTACAACTAA

mRNA sequence

ATGAAGCGGCGAACGGTGCATGTAGAAGATGTCCTTGAGGAAGCTCTTGATTTTAGTGTCAAGCATCTAAAAAATATTATAGCCAACACAAACTCCAAATTTGGTGCTGAAATTGTACCGTGCCTTGAAATAGCCACCAAGAAAAGACCTTCCAAGATTAAAAGCAAGAGAGTACATATCAATGTATCAAGAAAATCCTTCCCATTCTCAAACTTTGCTTGCTTTTCAAAACTGGATTTTAACATATTACAAAAATTTCATCAAAAGGAGCGGAAAGAACTCACAAGGTGGTGGAAAGATTTGAACGTCCAAACAAATTTTCCTTATGCCAGAGATAAAATTGTTGAGTGTTACTTTTGGACATTGGGTGCATACTTTGAACCTCGGTTTAATGTTGGAAGGAAAATATTAACCAAAGTAATCGTCATTTCTTCCATTTTAGATGACACATATGATACCTATGGAACATTTGAAGAACTCAAAATCTTCCCTCAAACAATTCAAAGATATTTGACAAAGATCTTAGCCTTAAAAAGCTTGACTTACAACTAA

Coding sequence (CDS)

ATGAAGCGGCGAACGGTGCATGTAGAAGATGTCCTTGAGGAAGCTCTTGATTTTAGTGTCAAGCATCTAAAAAATATTATAGCCAACACAAACTCCAAATTTGGTGCTGAAATTGTACCGTGCCTTGAAATAGCCACCAAGAAAAGACCTTCCAAGATTAAAAGCAAGAGAGTACATATCAATGTATCAAGAAAATCCTTCCCATTCTCAAACTTTGCTTGCTTTTCAAAACTGGATTTTAACATATTACAAAAATTTCATCAAAAGGAGCGGAAAGAACTCACAAGGTGGTGGAAAGATTTGAACGTCCAAACAAATTTTCCTTATGCCAGAGATAAAATTGTTGAGTGTTACTTTTGGACATTGGGTGCATACTTTGAACCTCGGTTTAATGTTGGAAGGAAAATATTAACCAAAGTAATCGTCATTTCTTCCATTTTAGATGACACATATGATACCTATGGAACATTTGAAGAACTCAAAATCTTCCCTCAAACAATTCAAAGATATTTGACAAAGATCTTAGCCTTAAAAAGCTTGACTTACAACTAA

Protein sequence

MKRRTVHVEDVLEEALDFSVKHLKNIIANTNSKFGAEIVPCLEIATKKRPSKIKSKRVHINVSRKSFPFSNFACFSKLDFNILQKFHQKERKELTRWWKDLNVQTNFPYARDKIVECYFWTLGAYFEPRFNVGRKILTKVIVISSILDDTYDTYGTFEELKIFPQTIQRYLTKILALKSLTYN

Homology

[Close]

BLAST of CmaCh16G008230.1 vs. Swiss-Prot
Match: GCSY1_CUCME ((+)-gamma-cadinene synthase OS=Cucumis melo PE=1 SV=1)

HSP 1 Score: 183.0 bits (463), Expect = 3.0e-45
Identity = 90/167 (53.89%), Postives = 116/167 (69.46%), Query Frame = 1


Query: 7   HVEDVLEEALDFSVKHLKNII---ANTNSKFGAEIVPCLEIATKKRPSKIKSKRVHINVS 66
           H E +LEEAL+F+  HL+  I   +N N  F +E+   L++  +K   +IK++       
Sbjct: 179 HGEALLEEALEFTTTHLQTYIHRYSNINPSFASEVSNALKLPIRKSVPRIKAREYLEIYQ 238

Query: 67  RKSFPFSNFACFSKLDFNILQKFHQKERKELTRWWKDLNVQTNFPYARDKIVECYFWTLG 126
           +          FSKLDFNILQK HQKE  E+ RWWKDL+V T FP+ARD+IVECYFWTLG
Sbjct: 239 QHPSHNETLLEFSKLDFNILQKLHQKELSEICRWWKDLDVPTKFPFARDRIVECYFWTLG 298

Query: 127 AYFEPRFNVGRKILTKVIVISSILDDTYDTYGTFEELKIFPQTIQRY 171
           AYFEP+++VGRK+LTKVI I+SILDD YD YGTFEEL++    IQR+
Sbjct: 299 AYFEPQYSVGRKMLTKVIAIASILDDIYDAYGTFEELQVLTPAIQRW 345

[Close]

BLAST of CmaCh16G008230.1 vs. Swiss-Prot
Match: TPSGB_SOLHA ((E,E)-germacrene B synthase OS=Solanum habrochaites GN=SSTLH1 PE=1 SV=1)

HSP 1 Score: 157.9 bits (398), Expect = 1.0e-37
Identity = 75/169 (44.38%), Postives = 116/169 (68.64%), Query Frame = 1


Query: 6   VHVEDVLEEALDFSVKHLKNIIA---NTNSKFGAEIVPCLEIATKKRPSKIKSKRVHINV 65
           V  E++LEEAL F+  HLK+I++   N N+    E+   L    +    +++++R +I++
Sbjct: 151 VRDEEILEEALAFTTTHLKSIVSTMSNNNNSLKVEVSEALTQPIRMTLPRMEARR-YISI 210

Query: 66  SRKSFPFSNFAC-FSKLDFNILQKFHQKERKELTRWWKDLNVQTNFPYARDKIVECYFWT 125
              +   ++    F+KLDFN+LQK HQ+E  +LTRWWKDL+    +PYARD++VECYFW 
Sbjct: 211 YENNDAHNHLLLKFAKLDFNMLQKLHQRELSDLTRWWKDLDFANKYPYARDRLVECYFWI 270

Query: 126 LGAYFEPRFNVGRKILTKVIVISSILDDTYDTYGTFEELKIFPQTIQRY 171
           LG YFEP+++  RK++TKV+ ++SI+DDT+D Y TF+EL+ F   IQR+
Sbjct: 271 LGVYFEPKYSRARKMMTKVLKMTSIIDDTFDAYATFDELEPFNDAIQRW 318

[Close]

BLAST of CmaCh16G008230.1 vs. Swiss-Prot
Match: TPS2_VALOF (Valerena-4,7(11)-diene synthase OS=Valeriana officinalis GN=TPS2 PE=1 SV=1)

HSP 1 Score: 156.0 bits (393), Expect = 4.0e-37
Identity = 70/167 (41.92%), Postives = 111/167 (66.47%), Query Frame = 1


Query: 6   VHVEDVLEEALDFSVKHLKNII--ANTNSKFGAEIVPCLEIATKKRPSKIKSKRVHINVS 65
           VH ED+LEEAL+F++  L+ ++  ++ ++   +E++  L +  +K  ++I+++       
Sbjct: 170 VHKEDILEEALEFTIYELEQVVKLSSNDTLLASEVIHALNMPIRKGLTRIEARHFISVYQ 229

Query: 66  RKSFPFSNFACFSKLDFNILQKFHQKERKELTRWWKDLNVQTNFPYARDKIVECYFWTLG 125
                      FSK+DFN+LQK HQ+E  +LT WW+ LNV    PYARD+ VECYFW LG
Sbjct: 230 HDKSHDETLLKFSKIDFNMLQKLHQRELADLTIWWEKLNVAEKMPYARDRFVECYFWGLG 289

Query: 126 AYFEPRFNVGRKILTKVIVISSILDDTYDTYGTFEELKIFPQTIQRY 171
            YFEP+++  RK+  KVI ++S++DDTYD+YGTF+EL +F   ++R+
Sbjct: 290 VYFEPQYSRARKMFVKVINLTSLIDDTYDSYGTFDELDLFTDAVKRW 336

[Close]

BLAST of CmaCh16G008230.1 vs. Swiss-Prot
Match: TPSGC_SOLLC (Germacrene C synthase OS=Solanum lycopersicum GN=SSTLE1 PE=1 SV=1)

HSP 1 Score: 155.2 bits (391), Expect = 6.8e-37
Identity = 74/169 (43.79%), Postives = 114/169 (67.46%), Query Frame = 1


Query: 6   VHVEDVLEEALDFSVKHLKNIIAN---TNSKFGAEIVPCLEIATKKRPSKIKSKRVHINV 65
           V  E++LEEAL F+  HL++I++N    N+    E+   L    +    ++ +++ +I++
Sbjct: 155 VRNEEILEEALTFTTTHLESIVSNLSNNNNSLKVEVGEALTQPIRMTLPRMGARK-YISI 214

Query: 66  SRKSFPFSNFAC-FSKLDFNILQKFHQKERKELTRWWKDLNVQTNFPYARDKIVECYFWT 125
              +    +    F+KLDFN+LQKFHQ+E  +LTRWWKDL+    +PYARD++VECYFW 
Sbjct: 215 YENNDAHHHLLLKFAKLDFNMLQKFHQRELSDLTRWWKDLDFANKYPYARDRLVECYFWI 274

Query: 126 LGAYFEPRFNVGRKILTKVIVISSILDDTYDTYGTFEELKIFPQTIQRY 171
           LG YFEP+++  RK++TKV+ ++SI+DDT+D Y TF+EL  F   IQR+
Sbjct: 275 LGVYFEPKYSRARKMMTKVLNLTSIIDDTFDAYATFDELVTFNDAIQRW 322

[Close]

BLAST of CmaCh16G008230.1 vs. Swiss-Prot
Match: TPS1_VALOF (Germacrene C/D synthase OS=Valeriana officinalis GN=TPS1 PE=1 SV=1)

HSP 1 Score: 154.5 bits (389), Expect = 1.2e-36
Identity = 75/167 (44.91%), Postives = 109/167 (65.27%), Query Frame = 1


Query: 6   VHVEDVLEEALDFSVKHLKNIIANTNSK--FGAEIVPCLEIATKKRPSKIKSKRVHINVS 65
           VH ED+LEEAL+F+V  L+ +I ++ S     +++V  L I  +K  ++++++       
Sbjct: 171 VHKEDILEEALEFTVAQLEQVIKSSLSDKVLLSQVVHALNIPIRKSLTRLEARYFISVYE 230

Query: 66  RKSFPFSNFACFSKLDFNILQKFHQKERKELTRWWKDLNVQTNFPYARDKIVECYFWTLG 125
           +          FSKLDFNILQK HQ+E  +LT WWK+LNV    PYARD++VECYFW L 
Sbjct: 231 QDKSCNETLLKFSKLDFNILQKLHQQEVADLTLWWKNLNVSEKVPYARDRLVECYFWALA 290

Query: 126 AYFEPRFNVGRKILTKVIVISSILDDTYDTYGTFEELKIFPQTIQRY 171
            YFEP+++  RK+  K+  + S++DDTYD+YGTFEEL +F    QR+
Sbjct: 291 EYFEPQYSRARKMSGKITALISLIDDTYDSYGTFEELALFTDAAQRW 337

[Close]

BLAST of CmaCh16G008230.1 vs. TrEMBL
Match: A0A0A0L2I8_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G097540 PE=3 SV=1)

HSP 1 Score: 187.6 bits (475), Expect = 1.4e-44
Identity = 92/167 (55.09%), Postives = 117/167 (70.06%), Query Frame = 1


Query: 7   HVEDVLEEALDFSVKHLK---NIIANTNSKFGAEIVPCLEIATKKRPSKIKSKRVHINVS 66
           H E +LEEAL+F+  HLK   ++ +N N  F +E+   L++  +K   +IK++       
Sbjct: 166 HGEALLEEALEFTTTHLKAYIHLYSNINPNFASEVSNALKLPIRKCVPRIKAREYFQIYQ 225

Query: 67  RKSFPFSNFACFSKLDFNILQKFHQKERKELTRWWKDLNVQTNFPYARDKIVECYFWTLG 126
           +          FSKLDFNILQK HQKE  E+ RWWKDL+V T FP+ARD+IVECYFWTLG
Sbjct: 226 QHPSHSETLLEFSKLDFNILQKLHQKELSEICRWWKDLDVPTKFPFARDRIVECYFWTLG 285

Query: 127 AYFEPRFNVGRKILTKVIVISSILDDTYDTYGTFEELKIFPQTIQRY 171
           AYFEP++NVGRK+LTKVI ISSILDD YD YGTFEEL++    IQR+
Sbjct: 286 AYFEPQYNVGRKMLTKVIAISSILDDIYDAYGTFEELQVLAPAIQRW 332

[Close]

BLAST of CmaCh16G008230.1 vs. TrEMBL
Match: Q66PX8_CUCSA (Beta-caryophyllene synthase OS=Cucumis sativus PE=2 SV=1)

HSP 1 Score: 179.9 bits (455), Expect = 2.9e-42
Identity = 87/167 (52.10%), Postives = 114/167 (68.26%), Query Frame = 1


Query: 7   HVEDVLEEALDFSVKHLK---NIIANTNSKFGAEIVPCLEIATKKRPSKIKSKRVHINVS 66
           H E +LEEAL+F+  HLK   ++ +N N  F +E+   L++  +K   ++K++       
Sbjct: 176 HGEALLEEALEFTTTHLKAYIHLYSNINPNFASEVSNALKLPIRKCVPRVKAREYFEIYQ 235

Query: 67  RKSFPFSNFACFSKLDFNILQKFHQKERKELTRWWKDLNVQTNFPYARDKIVECYFWTLG 126
           ++         FSKLDFNILQK HQKE  E+ RWWKDLNV TNFP+ARD+IVECYFW L 
Sbjct: 236 QQPSHNETLLTFSKLDFNILQKLHQKEIAEICRWWKDLNVSTNFPFARDRIVECYFWILS 295

Query: 127 AYFEPRFNVGRKILTKVIVISSILDDTYDTYGTFEELKIFPQTIQRY 171
            YFEP F  GRKILTKVI ++SI+DD YD YGTFEEL++F   I+R+
Sbjct: 296 IYFEPYFKFGRKILTKVIAMTSIMDDIYDAYGTFEELQLFTLAIKRW 342

[Close]

BLAST of CmaCh16G008230.1 vs. TrEMBL
Match: A0A0A0L579_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G097040 PE=3 SV=1)

HSP 1 Score: 179.9 bits (455), Expect = 2.9e-42
Identity = 87/167 (52.10%), Postives = 114/167 (68.26%), Query Frame = 1


Query: 7   HVEDVLEEALDFSVKHLK---NIIANTNSKFGAEIVPCLEIATKKRPSKIKSKRVHINVS 66
           H E +LEEAL+F+  HLK   ++ +N N  F +E+   L++  +K   ++K++       
Sbjct: 176 HGEALLEEALEFTTTHLKAYIHLYSNINPNFASEVSNALKLPIRKCVPRVKAREYFEIYQ 235

Query: 67  RKSFPFSNFACFSKLDFNILQKFHQKERKELTRWWKDLNVQTNFPYARDKIVECYFWTLG 126
           ++         FSKLDFNILQK HQKE  E+ RWWKDLNV TNFP+ARD+IVECYFW L 
Sbjct: 236 QQPSHNETLLTFSKLDFNILQKLHQKEIAEICRWWKDLNVSTNFPFARDRIVECYFWILS 295

Query: 127 AYFEPRFNVGRKILTKVIVISSILDDTYDTYGTFEELKIFPQTIQRY 171
            YFEP F  GRKILTKVI ++SI+DD YD YGTFEEL++F   I+R+
Sbjct: 296 IYFEPYFKFGRKILTKVIAMTSIMDDIYDAYGTFEELQLFTLAIKRW 342

[Close]

BLAST of CmaCh16G008230.1 vs. TrEMBL
Match: A0A0B4VEV4_CUCME (Sesquiterpene synthase Tps1 OS=Cucumis melo PE=2 SV=1)

HSP 1 Score: 179.1 bits (453), Expect = 4.9e-42
Identity = 87/168 (51.79%), Postives = 113/168 (67.26%), Query Frame = 1


Query: 6   VHVEDVLEEALDFSVKHLKNII---ANTNSKFGAEIVPCLEIATKKRPSKIKSKRVHINV 65
           VH E +LEEAL+F+  HL+  +   +N N  F +E+   L++  +K   ++K++      
Sbjct: 178 VHGEALLEEALEFTTTHLQTYVHRYSNINPSFASEVSNALKLPIRKGVPRMKAREYLEIY 237

Query: 66  SRKSFPFSNFACFSKLDFNILQKFHQKERKELTRWWKDLNVQTNFPYARDKIVECYFWTL 125
            +          FSKLDFN LQK HQKE  E+ RWWKDLNV TNFP+ARD+IVECYFW L
Sbjct: 238 QQHPSHNETLLTFSKLDFNALQKLHQKELAEICRWWKDLNVSTNFPFARDQIVECYFWIL 297

Query: 126 GAYFEPRFNVGRKILTKVIVISSILDDTYDTYGTFEELKIFPQTIQRY 171
             YFEPRF  GRKILTKVI ++SI+DD YD YGTFEEL++F   I+R+
Sbjct: 298 SIYFEPRFKFGRKILTKVIAMTSIMDDIYDAYGTFEELQVFTLAIKRW 345

[Close]

BLAST of CmaCh16G008230.1 vs. TrEMBL
Match: T2D1F3_9ROSI (Sesquiterpene synthase OS=Aquilaria sinensis PE=2 SV=1)

HSP 1 Score: 163.7 bits (413), Expect = 2.1e-37
Identity = 82/170 (48.24%), Postives = 114/170 (67.06%), Query Frame = 1


Query: 6   VHVEDVLEEALDFSVKHLKNIIANTNS--KFGAEIVPCLEIATKKRPSKIKSKRVHINVS 65
           +H ED+LE+A DFS KHLK+I  +T+S  K  A+    L+    K   ++++K  +I+V 
Sbjct: 152 LHGEDILEQAFDFSTKHLKSIAEDTSSSPKLAAQASQALKYPVPKNIPRLEAK-YYISV- 211

Query: 66  RKSFPFSN---FACFSKLDFNILQKFHQKERKELTRWWKDLNVQTNFPYARDKIVECYFW 125
              +P  N      F++LDFNILQK HQ E +EL RWWKDL++    PYARD+I E +FW
Sbjct: 212 ---YPLLNTPVVLAFAELDFNILQKLHQNELRELARWWKDLDIPRRLPYARDRITELFFW 271

Query: 126 TLGAYFEPRFNVGRKILTKVIVISSILDDTYDTYGTFEELKIFPQTIQRY 171
            L  Y+EP + +GRKILTKVI ++S+LDD YD YGT EEL++  + IQR+
Sbjct: 272 VLAVYYEPCYTLGRKILTKVIALTSLLDDMYDAYGTIEELELLTKAIQRW 316

[Close]

BLAST of CmaCh16G008230.1 vs. TAIR10
Match: AT5G23960.1 (AT5G23960.1 terpene synthase 21)

HSP 1 Score: 115.2 bits (287), Expect = 4.4e-26
Identity = 56/165 (33.94%), Postives = 93/165 (56.36%), Query Frame = 1


Query: 7   HVEDVLEEALDFSVKHLKNIIANTNSKFGAEIVPCLEIATKKRPSKIKSKRVHINVSRKS 66
           H ED+++EAL FS  HL+ I + ++      I   L+    K  S+I++++       + 
Sbjct: 158 HGEDIIDEALAFSRSHLEEISSRSSPHLAIRIKNALKHPYHKGISRIETRQYISYYEEEE 217

Query: 67  FPFSNFACFSKLDFNILQKFHQKERKELTRWWKDLNVQTNFPYARDKIVECYFWTLGAYF 126
                   F+K+DFN+LQ  H++E   +TRW  ++  ++   Y R +I E Y W+LG YF
Sbjct: 218 SCDPTLLEFAKIDFNLLQILHREELACVTRWHHEMEFKSKVTYTRHRITEAYLWSLGTYF 277

Query: 127 EPRFNVGRKILTKVIVISSILDDTYDTYGTFEELKIFPQTIQRYL 172
           EP+++  R I T  +++ + LDD YD YGT EEL++F   +  +L
Sbjct: 278 EPQYSQARVITTMALILFTALDDMYDAYGTMEELELFTDAMDEWL 322

[Close]

BLAST of CmaCh16G008230.1 vs. TAIR10
Match: AT3G14490.1 (AT3G14490.1 Terpenoid cyclases/Protein prenyltransferases superfamily protein)

HSP 1 Score: 109.4 bits (272), Expect = 2.4e-24
Identity = 59/170 (34.71%), Postives = 96/170 (56.47%), Query Frame = 1


Query: 9   EDVLEEALDFSVKHLKNIIANTNSKFGAEIVPCLEIATKKRPSKIKSKRVHINVSRKSFP 68
           ED+++EAL F+   L+++ +N      A   P L    +    + +   + I V+R+   
Sbjct: 211 EDIMDEALSFTRYRLESLTSNHT----ATASPHLSKHIQNALYRARYHNLEILVAREYIS 270

Query: 69  F--------SNFACFSKLDFNILQKFHQKERKELTRWWKDLNVQTNFPYARDKIVECYFW 128
           F             F+KL+FN  Q  + +E K+LT+WWK+L++ +  PY RD+IVE YF 
Sbjct: 271 FYEQEEDHDETLLKFAKLNFNYCQLHYIQELKDLTKWWKELDLASKLPYIRDRIVEVYFG 330

Query: 129 TLGAYFEPRFNVGRKILTKVIVISSILDDTYDTYGTFEELKIFPQTIQRY 171
            L  YFEPR+++GR I+TK+ +I ++ +DT D YGT  E+     + QR+
Sbjct: 331 ALALYFEPRYSLGRIIVTKITMIVTVFNDTCDAYGTLPEVTSLVDSFQRW 376

[Close]

BLAST of CmaCh16G008230.1 vs. TAIR10
Match: AT4G16730.1 (AT4G16730.1 terpene synthase 02)

HSP 1 Score: 107.8 bits (268), Expect = 7.0e-24
Identity = 47/115 (40.87%), Postives = 71/115 (61.74%), Query Frame = 1


Query: 57  RVHINVSRKSFPFSNFAC-FSKLDFNILQKFHQKERKELTRWWKDLNVQTNFPYARDKIV 116
           R +INV  K    +     F+ +DFN+LQ  HQ+E K ++ WW    +     + RD+I 
Sbjct: 197 RWYINVYEKKHEMNPLLLEFAAIDFNMLQANHQEELKLISSWWNSTGLMKQLDFVRDRIT 256

Query: 117 ECYFWTLGAYFEPRFNVGRKILTKVIVISSILDDTYDTYGTFEELKIFPQTIQRY 171
           E YFWT+G ++EP F   RKILTK+ ++  I+DD YD YGT EEL++F   ++++
Sbjct: 257 ESYFWTIGIFYEPEFKYCRKILTKIFMLIVIMDDIYDIYGTLEELELFTNVVEKW 311

[Close]

BLAST of CmaCh16G008230.1 vs. TAIR10
Match: AT3G25830.1 (AT3G25830.1 terpene synthase-like sequence-1,8-cineole)

HSP 1 Score: 107.5 bits (267), Expect = 9.1e-24
Identity = 44/96 (45.83%), Postives = 67/96 (69.79%), Query Frame = 1


Query: 75  FSKLDFNILQKFHQKERKELTRWWKDLNVQTNFPYARDKIVECYFWTLGAYFEPRFNVGR 134
           F+K+DFNI+Q  HQ++ K ++RWWKD  +    P+ RD+IVE YFWT+G  +EP+F   R
Sbjct: 269 FAKIDFNIVQAAHQEDLKYVSRWWKDTCLTNQLPFVRDRIVENYFWTVGLIYEPQFGYIR 328

Query: 135 KILTKVIVISSILDDTYDTYGTFEELKIFPQTIQRY 171
           +I+T V  + + +DD YD YGT EEL++F   ++ +
Sbjct: 329 RIMTIVNALVTTIDDIYDIYGTLEELELFTSMVENW 364

[Close]

BLAST of CmaCh16G008230.1 vs. TAIR10
Match: AT3G25820.1 (AT3G25820.1 terpene synthase-like sequence-1,8-cineole)

HSP 1 Score: 107.5 bits (267), Expect = 9.1e-24
Identity = 44/96 (45.83%), Postives = 67/96 (69.79%), Query Frame = 1


Query: 75  FSKLDFNILQKFHQKERKELTRWWKDLNVQTNFPYARDKIVECYFWTLGAYFEPRFNVGR 134
           F+K+DFNI+Q  HQ++ K ++RWWKD  +    P+ RD+IVE YFWT+G  +EP+F   R
Sbjct: 269 FAKIDFNIVQAAHQEDLKYVSRWWKDTCLTNQLPFVRDRIVENYFWTVGLIYEPQFGYIR 328

Query: 135 KILTKVIVISSILDDTYDTYGTFEELKIFPQTIQRY 171
           +I+T V  + + +DD YD YGT EEL++F   ++ +
Sbjct: 329 RIMTIVNALVTTIDDIYDIYGTLEELELFTSMVENW 364

[Close]

BLAST of CmaCh16G008230.1 vs. NCBI nr
Match: gi|778676208|ref|XP_011650545.1| (PREDICTED: (+)-gamma-cadinene synthase [Cucumis sativus])

HSP 1 Score: 187.6 bits (475), Expect = 2.0e-44
Identity = 92/167 (55.09%), Postives = 117/167 (70.06%), Query Frame = 1


Query: 7   HVEDVLEEALDFSVKHLK---NIIANTNSKFGAEIVPCLEIATKKRPSKIKSKRVHINVS 66
           H E +LEEAL+F+  HLK   ++ +N N  F +E+   L++  +K   +IK++       
Sbjct: 166 HGEALLEEALEFTTTHLKAYIHLYSNINPNFASEVSNALKLPIRKCVPRIKAREYFQIYQ 225

Query: 67  RKSFPFSNFACFSKLDFNILQKFHQKERKELTRWWKDLNVQTNFPYARDKIVECYFWTLG 126
           +          FSKLDFNILQK HQKE  E+ RWWKDL+V T FP+ARD+IVECYFWTLG
Sbjct: 226 QHPSHSETLLEFSKLDFNILQKLHQKELSEICRWWKDLDVPTKFPFARDRIVECYFWTLG 285

Query: 127 AYFEPRFNVGRKILTKVIVISSILDDTYDTYGTFEELKIFPQTIQRY 171
           AYFEP++NVGRK+LTKVI ISSILDD YD YGTFEEL++    IQR+
Sbjct: 286 AYFEPQYNVGRKMLTKVIAISSILDDIYDAYGTFEELQVLAPAIQRW 332

[Close]

BLAST of CmaCh16G008230.1 vs. NCBI nr
Match: gi|661902982|ref|NP_001284382.1| ((+)-gamma-cadinene synthase [Cucumis melo])

HSP 1 Score: 183.0 bits (463), Expect = 4.8e-43
Identity = 90/167 (53.89%), Postives = 116/167 (69.46%), Query Frame = 1


Query: 7   HVEDVLEEALDFSVKHLKNII---ANTNSKFGAEIVPCLEIATKKRPSKIKSKRVHINVS 66
           H E +LEEAL+F+  HL+  I   +N N  F +E+   L++  +K   +IK++       
Sbjct: 179 HGEALLEEALEFTTTHLQTYIHRYSNINPSFASEVSNALKLPIRKSVPRIKAREYLEIYQ 238

Query: 67  RKSFPFSNFACFSKLDFNILQKFHQKERKELTRWWKDLNVQTNFPYARDKIVECYFWTLG 126
           +          FSKLDFNILQK HQKE  E+ RWWKDL+V T FP+ARD+IVECYFWTLG
Sbjct: 239 QHPSHNETLLEFSKLDFNILQKLHQKELSEICRWWKDLDVPTKFPFARDRIVECYFWTLG 298

Query: 127 AYFEPRFNVGRKILTKVIVISSILDDTYDTYGTFEELKIFPQTIQRY 171
           AYFEP+++VGRK+LTKVI I+SILDD YD YGTFEEL++    IQR+
Sbjct: 299 AYFEPQYSVGRKMLTKVIAIASILDDIYDAYGTFEELQVLTPAIQRW 345

[Close]

BLAST of CmaCh16G008230.1 vs. NCBI nr
Match: gi|700201056|gb|KGN56189.1| (hypothetical protein Csa_3G097040 [Cucumis sativus])

HSP 1 Score: 179.9 bits (455), Expect = 4.1e-42
Identity = 87/167 (52.10%), Postives = 114/167 (68.26%), Query Frame = 1


Query: 7   HVEDVLEEALDFSVKHLK---NIIANTNSKFGAEIVPCLEIATKKRPSKIKSKRVHINVS 66
           H E +LEEAL+F+  HLK   ++ +N N  F +E+   L++  +K   ++K++       
Sbjct: 176 HGEALLEEALEFTTTHLKAYIHLYSNINPNFASEVSNALKLPIRKCVPRVKAREYFEIYQ 235

Query: 67  RKSFPFSNFACFSKLDFNILQKFHQKERKELTRWWKDLNVQTNFPYARDKIVECYFWTLG 126
           ++         FSKLDFNILQK HQKE  E+ RWWKDLNV TNFP+ARD+IVECYFW L 
Sbjct: 236 QQPSHNETLLTFSKLDFNILQKLHQKEIAEICRWWKDLNVSTNFPFARDRIVECYFWILS 295

Query: 127 AYFEPRFNVGRKILTKVIVISSILDDTYDTYGTFEELKIFPQTIQRY 171
            YFEP F  GRKILTKVI ++SI+DD YD YGTFEEL++F   I+R+
Sbjct: 296 IYFEPYFKFGRKILTKVIAMTSIMDDIYDAYGTFEELQLFTLAIKRW 342

[Close]

BLAST of CmaCh16G008230.1 vs. NCBI nr
Match: gi|793420197|ref|NP_001292628.1| ((+)-gamma-cadinene synthase [Cucumis sativus])

HSP 1 Score: 179.9 bits (455), Expect = 4.1e-42
Identity = 87/167 (52.10%), Postives = 114/167 (68.26%), Query Frame = 1


Query: 7   HVEDVLEEALDFSVKHLK---NIIANTNSKFGAEIVPCLEIATKKRPSKIKSKRVHINVS 66
           H E +LEEAL+F+  HLK   ++ +N N  F +E+   L++  +K   ++K++       
Sbjct: 176 HGEALLEEALEFTTTHLKAYIHLYSNINPNFASEVSNALKLPIRKCVPRVKAREYFEIYQ 235

Query: 67  RKSFPFSNFACFSKLDFNILQKFHQKERKELTRWWKDLNVQTNFPYARDKIVECYFWTLG 126
           ++         FSKLDFNILQK HQKE  E+ RWWKDLNV TNFP+ARD+IVECYFW L 
Sbjct: 236 QQPSHNETLLTFSKLDFNILQKLHQKEIAEICRWWKDLNVSTNFPFARDRIVECYFWILS 295

Query: 127 AYFEPRFNVGRKILTKVIVISSILDDTYDTYGTFEELKIFPQTIQRY 171
            YFEP F  GRKILTKVI ++SI+DD YD YGTFEEL++F   I+R+
Sbjct: 296 IYFEPYFKFGRKILTKVIAMTSIMDDIYDAYGTFEELQLFTLAIKRW 342

[Close]

BLAST of CmaCh16G008230.1 vs. NCBI nr
Match: gi|745672488|gb|AJD19682.1| (sesquiterpene synthase Tps1 [Cucumis melo])

HSP 1 Score: 179.1 bits (453), Expect = 7.0e-42
Identity = 87/168 (51.79%), Postives = 113/168 (67.26%), Query Frame = 1


Query: 6   VHVEDVLEEALDFSVKHLKNII---ANTNSKFGAEIVPCLEIATKKRPSKIKSKRVHINV 65
           VH E +LEEAL+F+  HL+  +   +N N  F +E+   L++  +K   ++K++      
Sbjct: 178 VHGEALLEEALEFTTTHLQTYVHRYSNINPSFASEVSNALKLPIRKGVPRMKAREYLEIY 237

Query: 66  SRKSFPFSNFACFSKLDFNILQKFHQKERKELTRWWKDLNVQTNFPYARDKIVECYFWTL 125
            +          FSKLDFN LQK HQKE  E+ RWWKDLNV TNFP+ARD+IVECYFW L
Sbjct: 238 QQHPSHNETLLTFSKLDFNALQKLHQKELAEICRWWKDLNVSTNFPFARDQIVECYFWIL 297

Query: 126 GAYFEPRFNVGRKILTKVIVISSILDDTYDTYGTFEELKIFPQTIQRY 171
             YFEPRF  GRKILTKVI ++SI+DD YD YGTFEEL++F   I+R+
Sbjct: 298 SIYFEPRFKFGRKILTKVIAMTSIMDDIYDAYGTFEELQVFTLAIKRW 345

The following BLAST results are available for this feature:

BLAST of CmaCh16G008230.1 vs. Swiss-Prot


Match Name	E-value	Identity	Description
GCSY1_CUCME	3.0e-45	53.89	(+)-gamma-cadinene synthase OS=Cucumis melo PE=1 SV=1	[more]
TPSGB_SOLHA	1.0e-37	44.38	(E,E)-germacrene B synthase OS=Solanum habrochaites GN=SSTLH1 PE=1 SV=1	[more]
TPS2_VALOF	4.0e-37	41.92	Valerena-4,7(11)-diene synthase OS=Valeriana officinalis GN=TPS2 PE=1 SV=1	[more]
TPSGC_SOLLC	6.8e-37	43.79	Germacrene C synthase OS=Solanum lycopersicum GN=SSTLE1 PE=1 SV=1	[more]
TPS1_VALOF	1.2e-36	44.91	Germacrene C/D synthase OS=Valeriana officinalis GN=TPS1 PE=1 SV=1	[more]

BLAST of CmaCh16G008230.1 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0L2I8_CUCSA	1.4e-44	55.09	Uncharacterized protein OS=Cucumis sativus GN=Csa_3G097540 PE=3 SV=1	[more]
Q66PX8_CUCSA	2.9e-42	52.10	Beta-caryophyllene synthase OS=Cucumis sativus PE=2 SV=1	[more]
A0A0A0L579_CUCSA	2.9e-42	52.10	Uncharacterized protein OS=Cucumis sativus GN=Csa_3G097040 PE=3 SV=1	[more]
A0A0B4VEV4_CUCME	4.9e-42	51.79	Sesquiterpene synthase Tps1 OS=Cucumis melo PE=2 SV=1	[more]
T2D1F3_9ROSI	2.1e-37	48.24	Sesquiterpene synthase OS=Aquilaria sinensis PE=2 SV=1	[more]

BLAST of CmaCh16G008230.1 vs. TAIR10


Match Name	E-value	Identity	Description
AT5G23960.1	4.4e-26	33.94	terpene synthase 21	[more]
AT3G14490.1	2.4e-24	34.71	Terpenoid cyclases/Protein prenyltransferases superfamily protein	[more]
AT4G16730.1	7.0e-24	40.87	terpene synthase 02	[more]
AT3G25830.1	9.1e-24	45.83	terpene synthase-like sequence-1,8-cineole	[more]
AT3G25820.1	9.1e-24	45.83	terpene synthase-like sequence-1,8-cineole	[more]

BLAST of CmaCh16G008230.1 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|778676208\|ref\|XP_011650545.1\|	2.0e-44	55.09	PREDICTED: (+)-gamma-cadinene synthase [Cucumis sativus]	[more]
gi\|661902982\|ref\|NP_001284382.1\|	4.8e-43	53.89	(+)-gamma-cadinene synthase [Cucumis melo]	[more]
gi\|700201056\|gb\|KGN56189.1\|	4.1e-42	52.10	hypothetical protein Csa_3G097040 [Cucumis sativus]	[more]
gi\|793420197\|ref\|NP_001292628.1\|	4.1e-42	52.10	(+)-gamma-cadinene synthase [Cucumis sativus]	[more]
gi\|745672488\|gb\|AJD19682.1\|	7.0e-42	51.79	sesquiterpene synthase Tps1 [Cucumis melo]	[more]

Annotated Terms

The following terms have been associated with this mRNA:

Vocabulary: **INTERPRO**
Term	Definition
IPR001906	Terpene_synth_N
IPR005630	Terpene_synthase_metal-bd
IPR008949	Isoprenoid_synthase_dom_sf

Vocabulary: **Biological Process**
Term	Definition
GO:0008152	metabolic process

Vocabulary: **Molecular Function**
Term	Definition
GO:0010333	terpene synthase activity
GO:0016829	lyase activity
GO:0000287	magnesium ion binding

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0008152	metabolic process
biological_process	GO:0045338	farnesyl diphosphate metabolic process
biological_process	GO:0051762	sesquiterpene biosynthetic process
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0016829	lyase activity
molecular_function	GO:0000287	magnesium ion binding
molecular_function	GO:0010333	terpene synthase activity
molecular_function	GO:0010334	sesquiterpene synthase activity

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
CmaCh16G008230	CmaCh16G008230	gene

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
CmaCh16G008230.1	CmaCh16G008230.1-protein	polypeptide

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CmaCh16G008230.1.exon.1	CmaCh16G008230.1.exon.1	exon
CmaCh16G008230.1.exon.2	CmaCh16G008230.1.exon.2	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CmaCh16G008230.1.CDS.1	CmaCh16G008230.1.CDS.1	CDS
CmaCh16G008230.1.CDS.2	CmaCh16G008230.1.CDS.2	CDS

InterPro

Analysis Name: InterPro Annotations of Cucurbita maxima
Date Performed: 2017-05-20


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001906	Terpene synthase, N-terminal domain	GENE3D	G3DSA:1.50.30.10		coord: 6..76 score: 1.
IPR005630	Terpene synthase, metal-binding domain	PFAM	PF03936	Terpene_synth_C	coord: 75..172 score: 8.7
IPR008949	Isoprenoid synthase domain	GENE3D	G3DSA:1.10.600.10		coord: 77..170 score: 4.0
IPR008949	Isoprenoid synthase domain	unknown	SSF48576	Terpenoid synthases	coord: 75..170 score: 2.76
None	No IPR available	PANTHER	PTHR31225	FAMILY NOT NAMED	coord: 6..170 score: 7.5
None	No IPR available	PANTHER	PTHR31225:SF15	ALPHA-HUMULENE/(-)-(E)-BETA-CARYOPHYLLENE SYNTHASE	coord: 6..170 score: 7.5