Cp4.1LG00g00300 (gene) Cucurbita pepo (Zucchini)

NameCp4.1LG00g00300
Typegene
OrganismCucurbita pepo (Cucurbita pepo (Zucchini))
Description(+)-gamma-cadinene synthase
LocationCp4.1LG00 : 513921 .. 515648 (+)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAGGGTGCATGGAGAAGATATCCTTGAGGAAGCGCTTGATTTTAGTGTCAAATATCTAAGAGATGTTACAACCAACTCAAACCCCAAATTTGCTGATGAAGTTCAAAGTGCCTTGAAATGGCCACTAAGAAAAACCCTTCCAAGACTAAAGGCAAGAGCGTACGTAACAATGTATCAAGAAGATCCTTCCCATTCTCAAACTTTGCTTGCTTTTTCAAAATTGGATTTCAACGTTTTACAGAAACTTCACCGAAAGGAATTGAAAGAACTCACCAGGTTTATATTTATTTATATGCTTTTTATTCTGCTTAGAGTATTTTTTTAATTTTTTTTCTAATGGTCAAAATTTCAAGTTTATAAAATAATGAAATTTAATGTATTATTTTATAGGTGGTGGAAGGATTTGGAAGTCCCAACAAACTTTCCTTTTGCTCGAGATAGAATTGTTGAGAGTTACTTTTGGACATTGGGTGCATACTTTGAACCACATTTCGGTGTTGGAAGGAAAATTTTAACTAAAGTAATCGTCATTTCTTCAATTTTAGATGATATATATGATGCCTACGGAACGTTTGAAGAACTCCAAATTTTTACTACGGCAATCCAAAGGTTATTCGAATTAGTTACGGTTCATTATGATTTTATTTTTAAAGTTCAATTTATATGCTAATATAATAAACTTACCTATTTACCTTGTGATTACATTTGAATTTTTTTTTAAAATTATTAATTTGGTTAGAAAAATAGATTTGAAAAATTGGTGTAAAAAGAATGATTTATTAAACATAGAAAATAGAAAATATAAAATACAAAAAGAAGTTTTTGCTTAAATCAAATCATATTCAAAATTAAATTCGCTTTTTTATCTATGATAAGCTTACCGAATTGCAGGTGGGATAGAAGTATGGTTAACGTGCTCCCAAAATACATGAAACATTTTTATATAGCAATGTTAGATGTTTTCGAAGAAATAAGCAAAGAAATAGGCAAGGATGAAAGTTCACTCCACATTCGTACGGCCAGAGAAGGAGTAAACTTTCTTTTTCTTCTTCTATTTTCTTTCCCTCCTTTCTATACATGTATATAAGAGTTCTCATTTTCATTTATTTTTACAGATAAAAAAGTTAGCTCAATCCTACTTTAAAGAAGCTAAATGGTTGAACAAAAAATAAAAACCAAATTTTAATGAGTACATGGAATTGGCGCTGTGTAGTACAGGGTATACCTTCATTATAAGCATTTCATTTCTTGGCATGGGTGACATAGTAACGAACGAGGTACTAGAATGGCTTTCTAGAAGACCTAAAATTGTTAAAGCCTCAGCCGTCATTTGTCGACTCATGGACGACGTCGTTTCCCACAAGGTATATATTGTTTACTATCCTTTAAAAGCAACACAAACAAAGTTATAATTATATGTTGTTCTTTTTAGTTTGAGCAAGAAAGGGAGCATGTTACTTCTGCAGTTGAATGTTACATGGAGCAATATGGTTGTTCAGAGAAAGAAGCTTGTGTTGAACTTCATAAACAAGTGGTTGATGCATGGAAGGACATAAATGAAGCATGTCTTTATCCAATCGATGTTCCAATGCGTATTCTTATGCGTGTGGTGAATCTTTCTAGAGTTATAAACGTGCTTTATTTAGACGAGGATGGATATACAAATGGAAGTGGTAGAACAAAACTTCTTATTGAATCTCTTCTTGTCAACTCAATACCTTGTTGA

mRNA sequence

ATGAGGGTGCATGGAGAAGATATCCTTGAGGAAGCGCTTGATTTTAGTGTCAAATATCTAAGAGATGTTACAACCAACTCAAACCCCAAATTTGCTGATGAAGTTCAAAGTGCCTTGAAATGGCCACTAAGAAAAACCCTTCCAAGACTAAAGGCAAGAGCGTACGTAACAATGTATCAAGAAGATCCTTCCCATTCTCAAACTTTGCTTGCTTTTTCAAAATTGGATTTCAACGTTTTACAGAAACTTCACCGAAAGGAATTGAAAGAACTCACCAGTACAGGGTATACCTTCATTATAAGCATTTCATTTCTTGGCATGGGTGACATAGTAACGAACGAGGTACTAGAATGGCTTTCTAGAAGACCTAAAATTGTTAAAGCCTCAGCCGTCATTTGTCGACTCATGGACGACGTCGTTTCCCACAAGTTTGAGCAAGAAAGGGAGCATGTTACTTCTGCAGTTGAATGTTACATGGAGCAATATGGTTGTTCAGAGAAAGAAGCTTGTGTTGAACTTCATAAACAAGTGGTTGATGCATGGAAGGACATAAATGAAGCATGTCTTTATCCAATCGATGTTCCAATGCGTATTCTTATGCGTGTGGTGAATCTTTCTAGAGTTATAAACGTGCTTTATTTAGACGAGGATGGATATACAAATGGAAGTGGTAGAACAAAACTTCTTATTGAATCTCTTCTTGTCAACTCAATACCTTGTTGA

Coding sequence (CDS)

ATGAGGGTGCATGGAGAAGATATCCTTGAGGAAGCGCTTGATTTTAGTGTCAAATATCTAAGAGATGTTACAACCAACTCAAACCCCAAATTTGCTGATGAAGTTCAAAGTGCCTTGAAATGGCCACTAAGAAAAACCCTTCCAAGACTAAAGGCAAGAGCGTACGTAACAATGTATCAAGAAGATCCTTCCCATTCTCAAACTTTGCTTGCTTTTTCAAAATTGGATTTCAACGTTTTACAGAAACTTCACCGAAAGGAATTGAAAGAACTCACCAGTACAGGGTATACCTTCATTATAAGCATTTCATTTCTTGGCATGGGTGACATAGTAACGAACGAGGTACTAGAATGGCTTTCTAGAAGACCTAAAATTGTTAAAGCCTCAGCCGTCATTTGTCGACTCATGGACGACGTCGTTTCCCACAAGTTTGAGCAAGAAAGGGAGCATGTTACTTCTGCAGTTGAATGTTACATGGAGCAATATGGTTGTTCAGAGAAAGAAGCTTGTGTTGAACTTCATAAACAAGTGGTTGATGCATGGAAGGACATAAATGAAGCATGTCTTTATCCAATCGATGTTCCAATGCGTATTCTTATGCGTGTGGTGAATCTTTCTAGAGTTATAAACGTGCTTTATTTAGACGAGGATGGATATACAAATGGAAGTGGTAGAACAAAACTTCTTATTGAATCTCTTCTTGTCAACTCAATACCTTGTTGA

Protein sequence

MRVHGEDILEEALDFSVKYLRDVTTNSNPKFADEVQSALKWPLRKTLPRLKARAYVTMYQEDPSHSQTLLAFSKLDFNVLQKLHRKELKELTSTGYTFIISISFLGMGDIVTNEVLEWLSRRPKIVKASAVICRLMDDVVSHKFEQEREHVTSAVECYMEQYGCSEKEACVELHKQVVDAWKDINEACLYPIDVPMRILMRVVNLSRVINVLYLDEDGYTNGSGRTKLLIESLLVNSIPC
BLAST of Cp4.1LG00g00300 vs. Swiss-Prot
Match: GCSY1_CUCME ((+)-gamma-cadinene synthase OS=Cucumis melo PE=1 SV=1)

HSP 1 Score: 231.9 bits (590), Expect = 7.5e-60
Identity = 113/167 (67.66%), Postives = 135/167 (80.84%), Query Frame = 1

Query: 80  LQKLHRKELKE-----LTSTGYTFIISISFLGMGD-IVTNEVLEWLSRRPKIVKASAVIC 139
           L K ++   KE     L +TGYT +ISISFLG+GD IVTNEVL+WLS  P+I+KAS +IC
Sbjct: 405 LNKEYKPSFKEYMELALKTTGYTMLISISFLGLGDHIVTNEVLQWLSNGPQIIKASTIIC 464

Query: 140 RLMDDVVSHKFEQEREHVTSAVECYMEQYGCSEKEACVELHKQVVDAWKDINEACLYPID 199
           RLMDD+ SHKFEQEREHV SAVECYM+QY CSE+EAC+ELHK+VVDAWKD NEA   P +
Sbjct: 465 RLMDDIASHKFEQEREHVASAVECYMKQYDCSEEEACIELHKEVVDAWKDTNEAFYRPFN 524

Query: 200 VPMRILMRVVNLSRVINVLYLDEDGYTNGSGRTKLLIESLLVNSIPC 241
           VP+ +LMRV+N SRVIN+LYLDEDGYTN    TK LI+SLLV+ +PC
Sbjct: 525 VPVPVLMRVLNFSRVINLLYLDEDGYTNAKSGTKFLIKSLLVDPLPC 571

BLAST of Cp4.1LG00g00300 vs. Swiss-Prot
Match: TPSGD_VITVI ((-)-germacrene D synthase OS=Vitis vinifera GN=VIT_19s0014g04930 PE=1 SV=1)

HSP 1 Score: 177.2 bits (448), Expect = 2.2e-43
Identity = 88/192 (45.83%), Postives = 129/192 (67.19%), Query Frame = 1

Query: 51  KARAYVTMYQEDPSHSQTLLAFSKLDFNVLQKLHRKELK---ELTSTGYTFIISISFLGM 110
           + R+Y   Y ++   +Q    + +  +  +Q++   E      L ++ Y+ + + SF+GM
Sbjct: 365 EGRSYRLYYAKEAMKNQVRAYYEEAKWLQVQQIPTMEEYMPVALVTSAYSMLATTSFVGM 424

Query: 111 GDIVTNEVLEWLSRRPKIVKASAVICRLMDDVVSHKFEQEREHVTSAVECYMEQYGCSEK 170
           GD VT E  +W+  +PKIV+ASA++CRLMDD+V HKFEQ+R HV SAVECYM+Q+G SE+
Sbjct: 425 GDAVTKESFDWIFSKPKIVRASAIVCRLMDDMVFHKFEQKRGHVASAVECYMKQHGASEQ 484

Query: 171 EACVELHKQVVDAWKDINEACLYPIDVPMRILMRVVNLSRVINVLYLDEDGYTNGSGRTK 230
           E   E  + V +AWKDINE CL P  VPM ILMRV+NL+RVI+V+Y +EDGYT+     K
Sbjct: 485 ETPNEFPQPVREAWKDINEECLIPTAVPMPILMRVLNLARVIDVIYKNEDGYTHFGAVLK 544

Query: 231 LLIESLLVNSIP 240
             + S+L++ +P
Sbjct: 545 DFVTSMLIDPVP 556

BLAST of Cp4.1LG00g00300 vs. Swiss-Prot
Match: STPS1_SANAL (Sesquiterpene synthase OS=Santalum album PE=1 SV=1)

HSP 1 Score: 172.9 bits (437), Expect = 4.1e-42
Identity = 81/146 (55.48%), Postives = 109/146 (74.66%), Query Frame = 1

Query: 91  LTSTGYTFIISISFLGMGDIVTNEVLEWLSRRPKIVKASAVICRLMDDVVSHKFEQEREH 150
           L ++GYTF+ +IS+LGMG+I + E  +WL   P +++AS  +CRLMDD+ SHKFEQER H
Sbjct: 410 LVTSGYTFLTTISYLGMGEIASKEAFDWLFSHPPVIEASESVCRLMDDMRSHKFEQERGH 469

Query: 151 VTSAVECYMEQYGCSEKEACVELHKQVVDAWKDINEACLYPIDVPMRILMRVVNLSRVIN 210
           V S +ECYM+QYG +E+EA  E  KQ+V AWKDINE CL P  VP  +LMR++NL+RVI+
Sbjct: 470 VASGIECYMKQYGVTEEEAHDEFRKQLVKAWKDINEECLRPYRVPKPLLMRILNLTRVID 529

Query: 211 VLYLDEDGYTNGSGRTKLLIESLLVN 237
           V+Y +EDGYT+     K  I SLL++
Sbjct: 530 VIYKNEDGYTHVKKAMKDNIASLLID 555

BLAST of Cp4.1LG00g00300 vs. Swiss-Prot
Match: SPIST_SANSP (Probable sesquiterpene synthase OS=Santalum spicatum GN=SesquiTPS PE=3 SV=1)

HSP 1 Score: 167.9 bits (424), Expect = 1.3e-40
Identity = 78/146 (53.42%), Postives = 109/146 (74.66%), Query Frame = 1

Query: 91  LTSTGYTFIISISFLGMGDIVTNEVLEWLSRRPKIVKASAVICRLMDDVVSHKFEQEREH 150
           L + GYTF+ +IS+LGMG+I + E  +WL  RP I++AS  +CRLMDD+ SH+FEQER H
Sbjct: 413 LVTAGYTFLTTISYLGMGEIASKEAFDWLFSRPPIIEASESVCRLMDDMRSHEFEQERGH 472

Query: 151 VTSAVECYMEQYGCSEKEACVELHKQVVDAWKDINEACLYPIDVPMRILMRVVNLSRVIN 210
           V S +ECYM+QYG +E+EA  + HK++V AWKDINE CL P  +P  +LMR+++L+RVI+
Sbjct: 473 VASGIECYMKQYGVTEEEAHDKFHKRLVKAWKDINEGCLRPYPMPKPLLMRILSLTRVID 532

Query: 211 VLYLDEDGYTNGSGRTKLLIESLLVN 237
           V+Y +ED YT+     K  I SLL++
Sbjct: 533 VIYKNEDWYTHVKKPMKDKIASLLID 558

BLAST of Cp4.1LG00g00300 vs. Swiss-Prot
Match: SAUSS_SANAS (Sesquiterpene synthase OS=Santalum austrocaledonicum PE=2 SV=1)

HSP 1 Score: 166.8 bits (421), Expect = 3.0e-40
Identity = 81/146 (55.48%), Postives = 106/146 (72.60%), Query Frame = 1

Query: 91  LTSTGYTFIISISFLGMGDIVTNEVLEWLSRRPKIVKASAVICRLMDDVVSHKFEQEREH 150
           L ++GYTF+ +IS+LGMG I + E  EWL   P I++AS  ICRLMDD+ SHKFEQER H
Sbjct: 410 LVTSGYTFLTTISYLGMGGIASKEAFEWLFSHPPIIEASESICRLMDDMSSHKFEQERGH 469

Query: 151 VTSAVECYMEQYGCSEKEACVELHKQVVDAWKDINEACLYPIDVPMRILMRVVNLSRVIN 210
           V S +ECYM+QYG  E+EA  E HK++V AWKDINE  L P  VP  +LMR++NL+RV++
Sbjct: 470 VASGIECYMKQYGVIEEEAHDEFHKRLVKAWKDINEGFLRPYAVPEPLLMRILNLTRVMD 529

Query: 211 VLYLDEDGYTNGSGRTKLLIESLLVN 237
           V+Y +ED YT+     K  I SLL++
Sbjct: 530 VIYKNEDSYTHVKKAMKDNIASLLID 555

BLAST of Cp4.1LG00g00300 vs. TrEMBL
Match: A0A0A0L2I8_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G097540 PE=3 SV=1)

HSP 1 Score: 232.6 bits (592), Expect = 4.9e-58
Identity = 114/167 (68.26%), Postives = 135/167 (80.84%), Query Frame = 1

Query: 80  LQKLHRKELKE-----LTSTGYTFIISISFLGMGD-IVTNEVLEWLSRRPKIVKASAVIC 139
           L K ++   KE     L +TGYT +ISISFLG+GD IVTNEVL+WLS  P+I+KAS +IC
Sbjct: 392 LNKKYKPSFKEYMELALNTTGYTLLISISFLGLGDHIVTNEVLQWLSNGPQIIKASTIIC 451

Query: 140 RLMDDVVSHKFEQEREHVTSAVECYMEQYGCSEKEACVELHKQVVDAWKDINEACLYPID 199
           RLMDD+ SHKFEQEREHV SAVE YMEQY CSE+EACVELHK+VVDAWKD NEA   P +
Sbjct: 452 RLMDDIASHKFEQEREHVASAVESYMEQYDCSEEEACVELHKEVVDAWKDTNEAFYRPFN 511

Query: 200 VPMRILMRVVNLSRVINVLYLDEDGYTNGSGRTKLLIESLLVNSIPC 241
           VP+ +LMRV+N SRV+N+LYLDEDGYTN   RTK LI+SLLV+ +PC
Sbjct: 512 VPLPVLMRVLNFSRVMNLLYLDEDGYTNAKSRTKFLIKSLLVDPLPC 558

BLAST of Cp4.1LG00g00300 vs. TrEMBL
Match: A0A0B4VEV4_CUCME (Sesquiterpene synthase Tps1 OS=Cucumis melo PE=2 SV=1)

HSP 1 Score: 191.0 bits (484), Expect = 1.6e-45
Identity = 91/148 (61.49%), Postives = 115/148 (77.70%), Query Frame = 1

Query: 91  LTSTGYTFIISISFLGMGDIVTNEVLEWLSRRPKIVKASAVICRLMDDVVSHKFEQEREH 150
           L S+GY  + +ISF+ MGDI T E+ +WL   PKI+KAS  I RLMDDVVS+KFE+EREH
Sbjct: 421 LASSGYELLSTISFVCMGDIATKEIFDWLFDCPKILKASTTISRLMDDVVSYKFEKEREH 480

Query: 151 VTSAVECYMEQYGCSEKEACVELHKQVVDAWKDINEACLYPIDVPMRILMRVVNLSRVIN 210
           V SAVECYM  YGCSE+E C +L KQV DAWK IN+ CL+P+ VP+  L+ V+NL+RV+ 
Sbjct: 481 VVSAVECYMNNYGCSEEETCADLLKQVEDAWKTINKCCLHPMTVPIPFLICVLNLTRVMA 540

Query: 211 VLYLDEDGYTNGSGRTKLLIESLLVNSI 239
           +LY  EDGYTN  GRTKLLI+SLL++ +
Sbjct: 541 LLYSGEDGYTNSKGRTKLLIQSLLIDPL 568

BLAST of Cp4.1LG00g00300 vs. TrEMBL
Match: A0A0A0L579_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G097040 PE=3 SV=1)

HSP 1 Score: 188.7 bits (478), Expect = 8.1e-45
Identity = 92/148 (62.16%), Postives = 114/148 (77.03%), Query Frame = 1

Query: 91  LTSTGYTFIISISFLGMGDIVTNEVLEWLSRRPKIVKASAVICRLMDDVVSHKFEQEREH 150
           L S+GY  + +ISF+ MGDI T EV EWL   PKI+KAS  I RLMDDVVS+KFE+EREH
Sbjct: 418 LASSGYELLSTISFVCMGDIATKEVFEWLFDCPKILKASTTISRLMDDVVSYKFEKEREH 477

Query: 151 VTSAVECYMEQYGCSEKEACVELHKQVVDAWKDINEACLYPIDVPMRILMRVVNLSRVIN 210
           + SAVECYM  +G SE E C EL KQV DAWK INE CL+P++VPM  L+ ++NL+RV+ 
Sbjct: 478 IVSAVECYMSNHGRSEDETCAELLKQVEDAWKTINECCLHPMNVPMPFLICLLNLTRVMA 537

Query: 211 VLYLDEDGYTNGSGRTKLLIESLLVNSI 239
           +LY  EDGYTN  GRTKLLI+SLL++ +
Sbjct: 538 LLYSHEDGYTNSKGRTKLLIQSLLIDPL 565

BLAST of Cp4.1LG00g00300 vs. TrEMBL
Match: A5BXR1_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_019788 PE=3 SV=1)

HSP 1 Score: 187.2 bits (474), Expect = 2.3e-44
Identity = 87/149 (58.39%), Postives = 115/149 (77.18%), Query Frame = 1

Query: 91  LTSTGYTFIISISFLGMGDIVTNEVLEWLSRRPKIVKASAVICRLMDDVVSHKFEQEREH 150
           L ++ Y+ + + SF+GMGD VT E  +W+  +PKIV+ASA++CRLMDD+VSHKFEQ+R H
Sbjct: 381 LVTSAYSMLATTSFVGMGDAVTKESFDWIFSKPKIVRASAIVCRLMDDMVSHKFEQKRGH 440

Query: 151 VTSAVECYMEQYGCSEKEACVELHKQVVDAWKDINEACLYPIDVPMRILMRVVNLSRVIN 210
           V SAVECYM+Q+G SE+E   E HKQV DAWKDINE CL P  VPM ILMRV+NL+RVI+
Sbjct: 441 VASAVECYMKQHGASEQETHNEFHKQVRDAWKDINEECLIPTAVPMPILMRVLNLARVID 500

Query: 211 VLYLDEDGYTNGSGRTKLLIESLLVNSIP 240
           V+Y +EDGYT+     K  + S+L++ +P
Sbjct: 501 VIYKNEDGYTHSGTVLKDFVTSMLIDPVP 529

BLAST of Cp4.1LG00g00300 vs. TrEMBL
Match: Q66PX8_CUCSA (Beta-caryophyllene synthase OS=Cucumis sativus PE=2 SV=1)

HSP 1 Score: 187.2 bits (474), Expect = 2.3e-44
Identity = 91/148 (61.49%), Postives = 114/148 (77.03%), Query Frame = 1

Query: 91  LTSTGYTFIISISFLGMGDIVTNEVLEWLSRRPKIVKASAVICRLMDDVVSHKFEQEREH 150
           L S+GY  + +ISF+ MGDI T EV EWL   P+I+KAS  I RLMDDVVS+KFE+EREH
Sbjct: 418 LASSGYELLSTISFVCMGDIATKEVFEWLFDCPQILKASTTISRLMDDVVSYKFEKEREH 477

Query: 151 VTSAVECYMEQYGCSEKEACVELHKQVVDAWKDINEACLYPIDVPMRILMRVVNLSRVIN 210
           + SAVECYM  +G SE E C EL KQV DAWK INE CL+P++VPM  L+ ++NL+RV+ 
Sbjct: 478 IVSAVECYMSNHGRSEDETCAELLKQVEDAWKTINECCLHPMNVPMPFLICLLNLTRVMA 537

Query: 211 VLYLDEDGYTNGSGRTKLLIESLLVNSI 239
           +LY  EDGYTN  GRTKLLI+SLL++ +
Sbjct: 538 LLYSHEDGYTNSKGRTKLLIQSLLIDPL 565

BLAST of Cp4.1LG00g00300 vs. TAIR10
Match: AT5G23960.1 (AT5G23960.1 terpene synthase 21)

HSP 1 Score: 131.3 bits (329), Expect = 7.7e-31
Identity = 71/158 (44.94%), Postives = 102/158 (64.56%), Query Frame = 1

Query: 87  ELKE--LTSTGYTFIISISFLGMGDIVTNEVLEWLSRRPKIVKASAVICRLMDDVVSHKF 146
           E KE  + S+GY  +I+++F+ M D+   +  EWLS  PKI  AS +I R  DD+ S++F
Sbjct: 393 EYKENAILSSGYYALIAMTFVRMTDVAKLDAFEWLSSHPKIRVASEIISRFTDDISSYEF 452

Query: 147 EQEREHVTSAVECYMEQYGCSEKEACVELHKQVVDAWKDINEACLYPIDVPMRILMRVVN 206
           E +REHV + ++CYM+Q+G S++ A   +   V DAWKD+N+  + P   P  +LMRV+N
Sbjct: 453 EHKREHVATGIDCYMQQFGVSKERAVEVMGNIVSDAWKDLNQELMRPHVFPFPLLMRVLN 512

Query: 207 LSRVINVLYLDEDGYTNGSGRTKLL---IESLLVNSIP 240
           LSRVI+V Y  +D YTN     KLL   I SLL+ +IP
Sbjct: 513 LSRVIDVFYRYQDAYTN----PKLLKEHIVSLLIETIP 546

BLAST of Cp4.1LG00g00300 vs. TAIR10
Match: AT4G20200.1 (AT4G20200.1 Terpenoid cyclases/Protein prenyltransferases superfamily protein)

HSP 1 Score: 110.2 bits (274), Expect = 1.8e-24
Identity = 73/233 (31.33%), Postives = 124/233 (53.22%), Query Frame = 1

Query: 10  EEALDFSVKYLRDVTT---NSNPKFADEV-QSALKWPLRKTLPRLKARAYVTMYQEDPSH 69
           ++A+D    YLR V     ++  +F  E+      + +  T+   KA A   +  E  + 
Sbjct: 381 DQAMDKQPDYLRFVLNFILDTFEEFKRELGPEERSYSVNATIEEFKAAAKANIDLEKWAQ 440

Query: 70  SQTLLAFSKLDFNVLQKLHRKELKELTSTGYTFIISISFLGMGDIVTNEVLEWLSRRPKI 129
           +  + +F +            E+ E+  T Y  +  I F+ MG + T E  EWL  RPK+
Sbjct: 441 ADHIPSFEEY----------MEVGEVEVTVYASLAGI-FMSMGKMATKEAFEWLKSRPKL 500

Query: 130 VKASAVICRLMDDVVSHKFEQEREHVTSAVECYMEQYGCSEKEACVELHKQVVDAWKDIN 189
           V+  ++  RLM+D++ ++ +  R +VT+AV CYM+QYG +++EA  EL+K VV A K +N
Sbjct: 501 VQYLSIKGRLMNDLMGYEDDMSRGYVTNAVNCYMKQYGVTKEEAFRELYKIVVAANKTLN 560

Query: 190 EACLYPIDVPMRILMRVVNLSRVINVLYLDEDGYTNGSGRTKLLIESLLVNSI 239
           E  L    VP  +L   ++L+R++ V Y   +G+TN  G+ K  + S+ V+ I
Sbjct: 561 EEFLSTTGVPHFLLKATIDLARMMTVAYNVNEGFTNPQGKIKEYMTSMFVDQI 602

BLAST of Cp4.1LG00g00300 vs. TAIR10
Match: AT1G48800.1 (AT1G48800.1 Terpenoid cyclases/Protein prenyltransferases superfamily protein)

HSP 1 Score: 106.7 bits (265), Expect = 2.0e-23
Identity = 55/139 (39.57%), Postives = 87/139 (62.59%), Query Frame = 1

Query: 98  FIISISFLGMGDIVTNEVLEWLSRRPKIVKASAVICRLMDDVVSHKFEQEREHVTSAVEC 157
           F ++   LGM +I   E  EWL  R K+V+A +   RL++D+  +K +  R +VT+++ C
Sbjct: 464 FTVAGILLGMENINKKEAYEWLIFRDKLVRAMSTKARLVNDLFGYKDDMRRGYVTNSINC 523

Query: 158 YMEQYGCSEKEACVELHKQVVDAWKDINEACLYPIDVPMRILMRVVNLSRVINVLYLDED 217
           Y +QYG +E+EA  +LH+ V D  K +NE  L PI+VP ++L  V++  R IN+ Y +ED
Sbjct: 524 YKKQYGVTEEEAFRKLHQMVADGDKMMNEEFLKPINVPHQVLKAVLDTLRAINICYDNED 583

Query: 218 GYTNGSGRTKLLIESLLVN 237
           G+T  +G  K  I S+ V+
Sbjct: 584 GFTRLNGNLKNYITSMYVD 602

BLAST of Cp4.1LG00g00300 vs. TAIR10
Match: AT3G29190.1 (AT3G29190.1 Terpenoid cyclases/Protein prenyltransferases superfamily protein)

HSP 1 Score: 101.3 bits (251), Expect = 8.6e-22
Identity = 55/156 (35.26%), Postives = 91/156 (58.33%), Query Frame = 1

Query: 86  KELKELTSTGYTFIISI--SFLGMGDIVTNEVLEWLSRRPKIVKASAVICRLMDDVVSHK 145
           +E  E+T  G T + ++  + + MG IV  E  +WL  RPKI++A A+  RLM+D+  +K
Sbjct: 445 EEYIEVTGVGITAMTTLMGAMMCMGKIVPKEDYKWLKSRPKIIQALAIKGRLMNDMKGYK 504

Query: 146 FEQEREHVTSAVECYMEQYGCSEKEACVELHKQVVDAWKDINEACLYPIDVPMRILMRVV 205
            +  R +  +AV CYM+QY  +E+EA  E  K V  A K +NE  L  + V   +L   +
Sbjct: 505 EDMSRGYAANAVTCYMKQYRVTEQEALKEFEKMVAVANKTVNEEFLTTMGVSRLVLKLAM 564

Query: 206 NLSRVINVLYLDEDGYTNGSGRTKLLIESLLVNSIP 240
            +  +I++ Y +++GYT+  G+ K  + +L V+ IP
Sbjct: 565 GVGLMISITYSEDEGYTHPEGKIKEKMTTLFVDQIP 600

BLAST of Cp4.1LG00g00300 vs. TAIR10
Match: AT5G44630.1 (AT5G44630.1 Terpenoid cyclases/Protein prenyltransferases superfamily protein)

HSP 1 Score: 100.9 bits (250), Expect = 1.1e-21
Identity = 53/139 (38.13%), Postives = 82/139 (58.99%), Query Frame = 1

Query: 100 ISISFLGMGDIVTNEVLEWLSRRPKIVKASAVICRLMDDVVSHKFEQEREHVTSAVECYM 159
           I+ S +G+G+I   E  EWL  RPK+V+      RLMDD+   + + E+ +  +A+  YM
Sbjct: 417 IACSIMGLGEIGKKEAFEWLISRPKLVRILGAKTRLMDDIADFEEDMEKGYTANALNYYM 476

Query: 160 EQYGCSEKEACVELHKQVVDAWKDINEACLYPIDVPMRILMRVVNLSRVINVLYLDEDGY 219
            ++G +++EA  EL K   D  K +NE CL    +P RILM+ VN +R ++VLY  +D Y
Sbjct: 477 NEHGVTKEEASRELEKMNGDMNKIVNEECLKITTMPRRILMQSVNYARSLDVLYTADDVY 536

Query: 220 TNGSGRTKLLIESLLVNSI 239
            +  G+ K  +  LLV+ I
Sbjct: 537 NHREGKLKEYMRLLLVDPI 555

BLAST of Cp4.1LG00g00300 vs. NCBI nr
Match: gi|778676208|ref|XP_011650545.1| (PREDICTED: (+)-gamma-cadinene synthase [Cucumis sativus])

HSP 1 Score: 232.6 bits (592), Expect = 7.0e-58
Identity = 114/167 (68.26%), Postives = 135/167 (80.84%), Query Frame = 1

Query: 80  LQKLHRKELKE-----LTSTGYTFIISISFLGMGD-IVTNEVLEWLSRRPKIVKASAVIC 139
           L K ++   KE     L +TGYT +ISISFLG+GD IVTNEVL+WLS  P+I+KAS +IC
Sbjct: 392 LNKKYKPSFKEYMELALNTTGYTLLISISFLGLGDHIVTNEVLQWLSNGPQIIKASTIIC 451

Query: 140 RLMDDVVSHKFEQEREHVTSAVECYMEQYGCSEKEACVELHKQVVDAWKDINEACLYPID 199
           RLMDD+ SHKFEQEREHV SAVE YMEQY CSE+EACVELHK+VVDAWKD NEA   P +
Sbjct: 452 RLMDDIASHKFEQEREHVASAVESYMEQYDCSEEEACVELHKEVVDAWKDTNEAFYRPFN 511

Query: 200 VPMRILMRVVNLSRVINVLYLDEDGYTNGSGRTKLLIESLLVNSIPC 241
           VP+ +LMRV+N SRV+N+LYLDEDGYTN   RTK LI+SLLV+ +PC
Sbjct: 512 VPLPVLMRVLNFSRVMNLLYLDEDGYTNAKSRTKFLIKSLLVDPLPC 558

BLAST of Cp4.1LG00g00300 vs. NCBI nr
Match: gi|661902982|ref|NP_001284382.1| ((+)-gamma-cadinene synthase [Cucumis melo])

HSP 1 Score: 231.9 bits (590), Expect = 1.2e-57
Identity = 113/167 (67.66%), Postives = 135/167 (80.84%), Query Frame = 1

Query: 80  LQKLHRKELKE-----LTSTGYTFIISISFLGMGD-IVTNEVLEWLSRRPKIVKASAVIC 139
           L K ++   KE     L +TGYT +ISISFLG+GD IVTNEVL+WLS  P+I+KAS +IC
Sbjct: 405 LNKEYKPSFKEYMELALKTTGYTMLISISFLGLGDHIVTNEVLQWLSNGPQIIKASTIIC 464

Query: 140 RLMDDVVSHKFEQEREHVTSAVECYMEQYGCSEKEACVELHKQVVDAWKDINEACLYPID 199
           RLMDD+ SHKFEQEREHV SAVECYM+QY CSE+EAC+ELHK+VVDAWKD NEA   P +
Sbjct: 465 RLMDDIASHKFEQEREHVASAVECYMKQYDCSEEEACIELHKEVVDAWKDTNEAFYRPFN 524

Query: 200 VPMRILMRVVNLSRVINVLYLDEDGYTNGSGRTKLLIESLLVNSIPC 241
           VP+ +LMRV+N SRVIN+LYLDEDGYTN    TK LI+SLLV+ +PC
Sbjct: 525 VPVPVLMRVLNFSRVINLLYLDEDGYTNAKSGTKFLIKSLLVDPLPC 571

BLAST of Cp4.1LG00g00300 vs. NCBI nr
Match: gi|659102820|ref|XP_008452330.1| (PREDICTED: (+)-gamma-cadinene synthase-like [Cucumis melo])

HSP 1 Score: 193.7 bits (491), Expect = 3.6e-46
Identity = 92/148 (62.16%), Postives = 116/148 (78.38%), Query Frame = 1

Query: 91  LTSTGYTFIISISFLGMGDIVTNEVLEWLSRRPKIVKASAVICRLMDDVVSHKFEQEREH 150
           L S+GY  + +ISF+ MGDI T E+ +WL   PKI+KAS  I RLMDDVVS+KFE+EREH
Sbjct: 421 LASSGYELLSTISFVCMGDIATEEIFDWLFDCPKILKASTTISRLMDDVVSYKFEKEREH 480

Query: 151 VTSAVECYMEQYGCSEKEACVELHKQVVDAWKDINEACLYPIDVPMRILMRVVNLSRVIN 210
           V SAVECYM  YGCSE+E C  L KQV DAWK+IN+ CL+P+ VP+  L+ V+NL+RV+ 
Sbjct: 481 VVSAVECYMNNYGCSEEETCANLLKQVKDAWKNINKCCLHPMTVPVPFLVCVLNLTRVMA 540

Query: 211 VLYLDEDGYTNGSGRTKLLIESLLVNSI 239
           +LY DEDGYTN  GRTKLLI+SLL++ +
Sbjct: 541 LLYSDEDGYTNSKGRTKLLIQSLLIDPL 568

BLAST of Cp4.1LG00g00300 vs. NCBI nr
Match: gi|745672488|gb|AJD19682.1| (sesquiterpene synthase Tps1 [Cucumis melo])

HSP 1 Score: 191.0 bits (484), Expect = 2.3e-45
Identity = 91/148 (61.49%), Postives = 115/148 (77.70%), Query Frame = 1

Query: 91  LTSTGYTFIISISFLGMGDIVTNEVLEWLSRRPKIVKASAVICRLMDDVVSHKFEQEREH 150
           L S+GY  + +ISF+ MGDI T E+ +WL   PKI+KAS  I RLMDDVVS+KFE+EREH
Sbjct: 421 LASSGYELLSTISFVCMGDIATKEIFDWLFDCPKILKASTTISRLMDDVVSYKFEKEREH 480

Query: 151 VTSAVECYMEQYGCSEKEACVELHKQVVDAWKDINEACLYPIDVPMRILMRVVNLSRVIN 210
           V SAVECYM  YGCSE+E C +L KQV DAWK IN+ CL+P+ VP+  L+ V+NL+RV+ 
Sbjct: 481 VVSAVECYMNNYGCSEEETCADLLKQVEDAWKTINKCCLHPMTVPIPFLICVLNLTRVMA 540

Query: 211 VLYLDEDGYTNGSGRTKLLIESLLVNSI 239
           +LY  EDGYTN  GRTKLLI+SLL++ +
Sbjct: 541 LLYSGEDGYTNSKGRTKLLIQSLLIDPL 568

BLAST of Cp4.1LG00g00300 vs. NCBI nr
Match: gi|302143188|emb|CBI20483.3| (unnamed protein product [Vitis vinifera])

HSP 1 Score: 189.1 bits (479), Expect = 8.9e-45
Identity = 93/192 (48.44%), Postives = 132/192 (68.75%), Query Frame = 1

Query: 51  KARAYVTMYQEDPSHSQTLLAFSKLDFNVLQKLHRKELK---ELTSTGYTFIISISFLGM 110
           + R+Y   Y ++   +Q    + +  +  +Q++   E      L ++ Y+ + + SF+GM
Sbjct: 365 EGRSYRLYYAKEAMKNQVRAYYEEAKWLQVQQIPTMEEYMPVALVTSAYSMLATTSFVGM 424

Query: 111 GDIVTNEVLEWLSRRPKIVKASAVICRLMDDVVSHKFEQEREHVTSAVECYMEQYGCSEK 170
           GD VT E  +W+  +PKIV+ASA++CRLMDD+VSHKFEQ+R HV SAVECYM+Q+G SE+
Sbjct: 425 GDAVTKESFDWIFSKPKIVRASAIVCRLMDDMVSHKFEQKRGHVASAVECYMKQHGASEQ 484

Query: 171 EACVELHKQVVDAWKDINEACLYPIDVPMRILMRVVNLSRVINVLYLDEDGYTNGSGRTK 230
           E   E HKQV DAWKDINE CL P  VPM ILMRV+NL+RVI+V+Y +EDGYT+     K
Sbjct: 485 ETHNEFHKQVRDAWKDINEECLIPTAVPMPILMRVLNLARVIDVIYKNEDGYTHSGTVLK 544

Query: 231 LLIESLLVNSIP 240
             + S+L++ +P
Sbjct: 545 DFVTSMLIDPVP 556

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
GCSY1_CUCME7.5e-6067.66(+)-gamma-cadinene synthase OS=Cucumis melo PE=1 SV=1[more]
TPSGD_VITVI2.2e-4345.83(-)-germacrene D synthase OS=Vitis vinifera GN=VIT_19s0014g04930 PE=1 SV=1[more]
STPS1_SANAL4.1e-4255.48Sesquiterpene synthase OS=Santalum album PE=1 SV=1[more]
SPIST_SANSP1.3e-4053.42Probable sesquiterpene synthase OS=Santalum spicatum GN=SesquiTPS PE=3 SV=1[more]
SAUSS_SANAS3.0e-4055.48Sesquiterpene synthase OS=Santalum austrocaledonicum PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0L2I8_CUCSA4.9e-5868.26Uncharacterized protein OS=Cucumis sativus GN=Csa_3G097540 PE=3 SV=1[more]
A0A0B4VEV4_CUCME1.6e-4561.49Sesquiterpene synthase Tps1 OS=Cucumis melo PE=2 SV=1[more]
A0A0A0L579_CUCSA8.1e-4562.16Uncharacterized protein OS=Cucumis sativus GN=Csa_3G097040 PE=3 SV=1[more]
A5BXR1_VITVI2.3e-4458.39Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_019788 PE=3 SV=1[more]
Q66PX8_CUCSA2.3e-4461.49Beta-caryophyllene synthase OS=Cucumis sativus PE=2 SV=1[more]
Match NameE-valueIdentityDescription
AT5G23960.17.7e-3144.94 terpene synthase 21[more]
AT4G20200.11.8e-2431.33 Terpenoid cyclases/Protein prenyltransferases superfamily protein[more]
AT1G48800.12.0e-2339.57 Terpenoid cyclases/Protein prenyltransferases superfamily protein[more]
AT3G29190.18.6e-2235.26 Terpenoid cyclases/Protein prenyltransferases superfamily protein[more]
AT5G44630.11.1e-2138.13 Terpenoid cyclases/Protein prenyltransferases superfamily protein[more]
Match NameE-valueIdentityDescription
gi|778676208|ref|XP_011650545.1|7.0e-5868.26PREDICTED: (+)-gamma-cadinene synthase [Cucumis sativus][more]
gi|661902982|ref|NP_001284382.1|1.2e-5767.66(+)-gamma-cadinene synthase [Cucumis melo][more]
gi|659102820|ref|XP_008452330.1|3.6e-4662.16PREDICTED: (+)-gamma-cadinene synthase-like [Cucumis melo][more]
gi|745672488|gb|AJD19682.1|2.3e-4561.49sesquiterpene synthase Tps1 [Cucumis melo][more]
gi|302143188|emb|CBI20483.3|8.9e-4548.44unnamed protein product [Vitis vinifera][more]
The following terms have been associated with this gene:
Vocabulary: Molecular Function
TermDefinition
GO:0000287magnesium ion binding
GO:0016829lyase activity
GO:0010333terpene synthase activity
Vocabulary: Biological Process
TermDefinition
GO:0008152metabolic process
Vocabulary: INTERPRO
TermDefinition
IPR008949Isoprenoid_synthase_dom_sf
IPR008930Terpenoid_cyclase/PrenylTrfase
IPR005630Terpene_synthase_metal-bd
IPR001906Terpene_synth_N
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008299 isoprenoid biosynthetic process
biological_process GO:0006721 terpenoid metabolic process
biological_process GO:0045338 farnesyl diphosphate metabolic process
biological_process GO:0051762 sesquiterpene biosynthetic process
biological_process GO:0016114 terpenoid biosynthetic process
biological_process GO:0008152 metabolic process
cellular_component GO:0005575 cellular_component
cellular_component GO:0005737 cytoplasm
molecular_function GO:0000287 magnesium ion binding
molecular_function GO:0010334 sesquiterpene synthase activity
molecular_function GO:0047461 (+)-delta-cadinene synthase activity
molecular_function GO:0016829 lyase activity
molecular_function GO:0010333 terpene synthase activity

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG00g00300.1Cp4.1LG00g00300.1mRNA


Analysis Name: InterPro Annotations of Cucurbita pepo
Date Performed: 2017-12-02
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001906Terpene synthase, N-terminal domainGENE3DG3DSA:1.50.30.10coord: 1..79
score: 3.3
IPR005630Terpene synthase, metal-binding domainPFAMPF03936Terpene_synth_Ccoord: 92..183
score: 1.7
IPR008930Terpenoid cyclases/protein prenyltransferase alpha-alpha toroidunknownSSF48239Terpenoid cyclases/Protein prenyltransferasescoord: 1..65
score: 2.28
IPR008949Isoprenoid synthase domainGENE3DG3DSA:1.10.600.10coord: 91..239
score: 3.2
IPR008949Isoprenoid synthase domainunknownSSF48576Terpenoid synthasescoord: 92..238
score: 2.88
NoneNo IPR availablePANTHERPTHR31225FAMILY NOT NAMEDcoord: 1..240
score: 2.1E
NoneNo IPR availablePANTHERPTHR31225:SF15ALPHA-HUMULENE/(-)-(E)-BETA-CARYOPHYLLENE SYNTHASEcoord: 1..240
score: 2.1E

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:

None