CmaCh08G001100 (gene) Cucurbita maxima (Rimu) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	CmaCh08G001100
Type	gene
Organism	Cucurbita maxima (Cucurbita maxima (Rimu))
Description	E3 ubiquitin-protein ligase
Location	Cma_Chr08 : 583263 .. 585687 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

TTCTGTCTGTCGTGTCGACCTATCCGAAATTGGGTCTGAAAAAGGAAGGGAAAAGAAAAAACCCCTTCGATTTTTAACTAATTTCTCGTTTTCTTATTAATAAATTTCCCCTCTCTCAATTTCTCCGATCTTTGAGCCCTCCGATCAACAATTTTCAACCATTTTTCCGATCATTCTTCAGATTCCGTCAAGCCGGCGATATTAATGGCGAAATCATCTGACCCATCAAAACCCATCAATGGATTCTGATGTTCTTCCGATTGTCGGAGCGGAATCATTAATCATTCCAAGTCGGAGAGGCTTATTATTTTGCGAAAATGGAGTTGGATGGCTTCGAACGCGCGCCGCCGTCCATGGACGTGATGGACGAGGAGGACAAGACCCTTCAATGTCACTGTCAGTTTCCTTCGATTTCCAAGCCTCGGATCAGCAATAACGACAACCCGACCACTACAAGTGTCCACGAGCTGCTCGAATGTCCTGTCTGCACTAATTCTATGTATCCTCCAATTCATCAGGTTCCTTCAAGTTCGTTGTTTTTTCCTGCTGTTTTTGTTTCAATTCGTTTGGTGTTTGTGGTTTCTGATCTGTTTGATTATGCTGAAGTTGTGGGTTTGTTGTTAGAGAAATGTTCTGTTTAATGAGGCCTTTGGTTCATGAAATGAGTATCAATTGTTAGTTAAACCCTTGCTGTTCTATATACTCATTAGCAAAATGAATAGCTTTTTTTGTTGTTTAGAGACAATTGTTGCTATCATTTGTTGTGTCCCTTTTTGGGGTTAGCTTAGTTAATACAAAGGAGGGGGAATAAGGGAATCCTTTTCTCAATCCTCCCCCTCGCAGATATTGCTCGTTATGGATCGTCGTCAGCCAAACGGTTTTAAAATTCATTTACTAGGGAGAAATTTCACACAAGTCATGACGGTGATACGTAACGGGTCAAAATGGACATTATCAACTAGTGGTAGGCTTGGGCTGTTACAAATGGTATCAGAGTCAGACACCGAGCTGTTTGTTAGTGAGGTCACTGAGTCGTCAAGGGGAAAGGATTATGAGATCCCGCATTAGTTAGAGAGGGGAACGAAACATTCCTTAAAGGGTGTGGAAACCTCTCCCTGGCAAACGCATTTTAAGATCGTGAGGCTGATTGCGATACGTAACGAGCTAAAGCAAACAATATTTGTGAGCTGTGGACTTGGACTGTTACAGGAAGTTTTTTATCGTTGTGTAAGATACATTGTAATAATGTTGGAAGGCTCCCCTCTAGGCTTTATAACCAGGATTTTATTATTCAGAACTCGGATTCTACCTTGAGATCTATGGCTTCATCAAATGAGAAGATATTGCTGAATGTTGTGAACTTATTGTTTTGTTTGTGCAGTGTCCTAATGGACATACCCTCTGTTCTTCGTGTAAAACGAAGGTAGACAACCGTTGCCCGACGTGCAGACAAGAGCTCGGTGATATTAGATGTCTAGCATTAGAAAAGATAGCCGAATCGCTTAAACTGGCTTGTAAATTCTGCACGTTCGGGTGCTCGGAGATATTGCCTTACTATAGTAAACTAAAACATGAATCTGCCTGTTACTTCAGACCATATACCTGCCCTTATGCTGGATCAGACTGCCCAGTTGATGGGGAGATTCCTTTCCTTGTTTCTCATCTTCGAGATGATCACAAAGTCGATATGCATTCCGGATGCACGTTTAATCATCGTTACGTAAAGGCTAATCCATGTGAAGTTGAAAACGCTATATGGATGTTAACTGTGAGTATCTGTTGAACCATTTCACGAGACGATATCCAAGATCACGTCTTACTTCTTACACGAGTTTCTGTTGATGGCTCTTATTTCACCTTTGTGCAGGTCTTCCACTGTTTTGACCAGTATTTCTGTCTTCATTTTGAAGCCTTTCAGCTTGGTATGGCACCAGTTTACATGGTGTTTCTCCGATTCATGGGCGATGAAACAGATGCCCGAAACTACAGTTACAGCCTCGAGATCGGTGGGAATGGCAGGAAGCTAACATGGGAAGGCAACCCTCGAAGCATTCGGGACAACCACAAGAAAGTCCGAGACAGCCACGATGGCCTCATTATACATCGAAACATGGCACTGTTCTTCTCGGGCGGGGAAAGGAAAGAGCTGAAGTTGCGGATCATGGGACGGATTTGGAAAGAACAAAATAAACAGTGATATTGTTGTTGAATCAGCTCATAAGTCTTTGCCAATCCTAACTTGGAGGCCCCAATCAATATTACAGTGTACACAAAGGTCTTGAAACCATCTTCGACTCCAACTTCTTGTAATTGGGAATAGCTTACATTACAACATTAAACATTGAAGCTTGTTTTGAAAACTGTGTTCTTACTTGTTCTTTTTCCAATAAGAAAAAAGAGAAGAAAAGTTCAGCTGAGAAACAAA

mRNA sequence

TTCTGTCTGTCGTGTCGACCTATCCGAAATTGGGTCTGAAAAAGGAAGGGAAAAGAAAAAACCCCTTCGATTTTTAACTAATTTCTCGTTTTCTTATTAATAAATTTCCCCTCTCTCAATTTCTCCGATCTTTGAGCCCTCCGATCAACAATTTTCAACCATTTTTCCGATCATTCTTCAGATTCCGTCAAGCCGGCGATATTAATGGCGAAATCATCTGACCCATCAAAACCCATCAATGGATTCTGATGTTCTTCCGATTGTCGGAGCGGAATCATTAATCATTCCAAGTCGGAGAGGCTTATTATTTTGCGAAAATGGAGTTGGATGGCTTCGAACGCGCGCCGCCGTCCATGGACGTGATGGACGAGGAGGACAAGACCCTTCAATGTCACTGTCAGTTTCCTTCGATTTCCAAGCCTCGGATCAGCAATAACGACAACCCGACCACTACAAGTGTCCACGAGCTGCTCGAATGTCCTGTCTGCACTAATTCTATGTATCCTCCAATTCATCAGTGTCCTAATGGACATACCCTCTGTTCTTCGTGTAAAACGAAGGTAGACAACCGTTGCCCGACGTGCAGACAAGAGCTCGGTGATATTAGATGTCTAGCATTAGAAAAGATAGCCGAATCGCTTAAACTGGCTTGTAAATTCTGCACGTTCGGGTGCTCGGAGATATTGCCTTACTATAGTAAACTAAAACATGAATCTGCCTGTTACTTCAGACCATATACCTGCCCTTATGCTGGATCAGACTGCCCAGTTGATGGGGAGATTCCTTTCCTTGTTTCTCATCTTCGAGATGATCACAAAGTCGATATGCATTCCGGATGCACGTTTAATCATCGTTACGTAAAGGCTAATCCATGTGAAGTTGAAAACGCTATATGGATGTTAACTGTCTTCCACTGTTTTGACCAGTATTTCTGTCTTCATTTTGAAGCCTTTCAGCTTGGTATGGCACCAGTTTACATGGTGTTTCTCCGATTCATGGGCGATGAAACAGATGCCCGAAACTACAGTTACAGCCTCGAGATCGGTGGGAATGGCAGGAAGCTAACATGGGAAGGCAACCCTCGAAGCATTCGGGACAACCACAAGAAAGTCCGAGACAGCCACGATGGCCTCATTATACATCGAAACATGGCACTGTTCTTCTCGGGCGGGGAAAGGAAAGAGCTGAAGTTGCGGATCATGGGACGGATTTGGAAAGAACAAAATAAACAGTGATATTGTTGTTGAATCAGCTCATAAGTCTTTGCCAATCCTAACTTGGAGGCCCCAATCAATATTACAGTGTACACAAAGGTCTTGAAACCATCTTCGACTCCAACTTCTTGTAATTGGGAATAGCTTACATTACAACATTAAACATTGAAGCTTGTTTTGAAAACTGTGTTCTTACTTGTTCTTTTTCCAATAAGAAAAAAGAGAAGAAAAGTTCAGCTGAGAAACAAA

Coding sequence (CDS)

ATGGAGTTGGATGGCTTCGAACGCGCGCCGCCGTCCATGGACGTGATGGACGAGGAGGACAAGACCCTTCAATGTCACTGTCAGTTTCCTTCGATTTCCAAGCCTCGGATCAGCAATAACGACAACCCGACCACTACAAGTGTCCACGAGCTGCTCGAATGTCCTGTCTGCACTAATTCTATGTATCCTCCAATTCATCAGTGTCCTAATGGACATACCCTCTGTTCTTCGTGTAAAACGAAGGTAGACAACCGTTGCCCGACGTGCAGACAAGAGCTCGGTGATATTAGATGTCTAGCATTAGAAAAGATAGCCGAATCGCTTAAACTGGCTTGTAAATTCTGCACGTTCGGGTGCTCGGAGATATTGCCTTACTATAGTAAACTAAAACATGAATCTGCCTGTTACTTCAGACCATATACCTGCCCTTATGCTGGATCAGACTGCCCAGTTGATGGGGAGATTCCTTTCCTTGTTTCTCATCTTCGAGATGATCACAAAGTCGATATGCATTCCGGATGCACGTTTAATCATCGTTACGTAAAGGCTAATCCATGTGAAGTTGAAAACGCTATATGGATGTTAACTGTCTTCCACTGTTTTGACCAGTATTTCTGTCTTCATTTTGAAGCCTTTCAGCTTGGTATGGCACCAGTTTACATGGTGTTTCTCCGATTCATGGGCGATGAAACAGATGCCCGAAACTACAGTTACAGCCTCGAGATCGGTGGGAATGGCAGGAAGCTAACATGGGAAGGCAACCCTCGAAGCATTCGGGACAACCACAAGAAAGTCCGAGACAGCCACGATGGCCTCATTATACATCGAAACATGGCACTGTTCTTCTCGGGCGGGGAAAGGAAAGAGCTGAAGTTGCGGATCATGGGACGGATTTGGAAAGAACAAAATAAACAGTGA

Protein sequence

MELDGFERAPPSMDVMDEEDKTLQCHCQFPSISKPRISNNDNPTTTSVHELLECPVCTNSMYPPIHQCPNGHTLCSSCKTKVDNRCPTCRQELGDIRCLALEKIAESLKLACKFCTFGCSEILPYYSKLKHESACYFRPYTCPYAGSDCPVDGEIPFLVSHLRDDHKVDMHSGCTFNHRYVKANPCEVENAIWMLTVFHCFDQYFCLHFEAFQLGMAPVYMVFLRFMGDETDARNYSYSLEIGGNGRKLTWEGNPRSIRDNHKKVRDSHDGLIIHRNMALFFSGGERKELKLRIMGRIWKEQNKQ

Homology

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BLAST of CmaCh08G001100 vs. Swiss-Prot
Match: SINA3_ARATH (E3 ubiquitin-protein ligase SINAT3 OS=Arabidopsis thaliana GN=SINAT3 PE=2 SV=1)

HSP 1 Score: 498.0 bits (1281), Expect = 7.1e-140
Identity = 233/315 (73.97%), Postives = 262/315 (83.17%), Query Frame = 1


Query: 1   MELDGFERAPPSMDVMDEEDKTLQCHCQFPSISKPRISNNDN-----------PTTTSVH 60
           M+LD  +    +MDV D+E+     H  + S+SK   +NN+            PTTTSVH
Sbjct: 1   MDLDSMD-CTSTMDVTDDEEIHQDRH-SYASVSKHHHTNNNTTNVNAAASGLLPTTTSVH 60

Query: 61  ELLECPVCTNSMYPPIHQCPNGHTLCSSCKTKVDNRCPTCRQELGDIRCLALEKIAESLK 120
           ELLECPVCTNSMYPPIHQC NGHTLCS+CK +V NRCPTCRQELGDIRCLALEK+AESL+
Sbjct: 61  ELLECPVCTNSMYPPIHQCHNGHTLCSTCKARVHNRCPTCRQELGDIRCLALEKVAESLE 120

Query: 121 LACKFCTFGCSEILPYYSKLKHESACYFRPYTCPYAGSDCPVDGEIPFLVSHLRDDHKVD 180
           L CK  + GC EI PYYSKLKHE+ C FRPY+CPYAGS+C V G+IPFLV+HLRDDHKVD
Sbjct: 121 LPCKHMSLGCPEIFPYYSKLKHETVCNFRPYSCPYAGSECSVTGDIPFLVAHLRDDHKVD 180

Query: 181 MHSGCTFNHRYVKANPCEVENAIWMLTVFHCFDQYFCLHFEAFQLGMAPVYMVFLRFMGD 240
           MHSGCTFNHRYVK+NP EVENA WMLTVFHCF QYFCLHFEAFQLGMAPVYM FLRFMGD
Sbjct: 181 MHSGCTFNHRYVKSNPREVENATWMLTVFHCFGQYFCLHFEAFQLGMAPVYMAFLRFMGD 240

Query: 241 ETDARNYSYSLEIGGNGRKLTWEGNPRSIRDNHKKVRDSHDGLIIHRNMALFFSGGERKE 300
           ET+ARNY+YSLE+GG GRKL WEG PRS+RD+H+KVRDSHDGLII RNMALFFSGG+RKE
Sbjct: 241 ETEARNYNYSLEVGGYGRKLIWEGTPRSVRDSHRKVRDSHDGLIIQRNMALFFSGGDRKE 300

Query: 301 LKLRIMGRIWKEQNK 305
           LKLR+ GRIWKEQ +
Sbjct: 301 LKLRVTGRIWKEQQQ 313

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BLAST of CmaCh08G001100 vs. Swiss-Prot
Match: SINA5_ARATH (E3 ubiquitin-protein ligase SINAT5 OS=Arabidopsis thaliana GN=SINAT5 PE=1 SV=2)

HSP 1 Score: 470.7 bits (1210), Expect = 1.2e-131
Identity = 218/291 (74.91%), Postives = 246/291 (84.54%), Query Frame = 1


Query: 12  SMDVMDEEDKTLQCHCQFPSISKPRISNNDNPTTTSVHELLECPVCTNSMYPPIHQCPNG 71
           S+D + ++D+  Q H QF S      +       TSV+ELLECPVCTNSMYPPIHQC NG
Sbjct: 5   SIDSVIDDDEIHQKH-QFSSTKSQGGATVVISPATSVYELLECPVCTNSMYPPIHQCHNG 64

Query: 72  HTLCSSCKTKVDNRCPTCRQELGDIRCLALEKIAESLKLACKFCTFGCSEILPYYSKLKH 131
           HTLCS+CK++V NRCPTCRQELGDIRCLALEK+AESL+L CK+   GC  I PYYSKLKH
Sbjct: 65  HTLCSTCKSRVHNRCPTCRQELGDIRCLALEKVAESLELPCKYYNLGCLGIFPYYSKLKH 124

Query: 132 ESACYFRPYTCPYAGSDCPVDGEIPFLVSHLRDDHKVDMHSGCTFNHRYVKANPCEVENA 191
           ES C FRPY+CPYAGS+C   G+I FLV+HLRDDHKVDMH+GCTFNHRYVK+NP EVENA
Sbjct: 125 ESQCNFRPYSCPYAGSECAAVGDITFLVAHLRDDHKVDMHTGCTFNHRYVKSNPREVENA 184

Query: 192 IWMLTVFHCFDQYFCLHFEAFQLGMAPVYMVFLRFMGDETDARNYSYSLEIGGNGRKLTW 251
            WMLTVF CF QYFCLHFEAFQLGMAPVYM FLRFMGDE DARNY+YSLE+GG+GRK TW
Sbjct: 185 TWMLTVFQCFGQYFCLHFEAFQLGMAPVYMAFLRFMGDEDDARNYTYSLEVGGSGRKQTW 244

Query: 252 EGNPRSIRDNHKKVRDSHDGLIIHRNMALFFSGGERKELKLRIMGRIWKEQ 303
           EG PRS+RD+H+KVRDSHDGLII RNMALFFSGG++KELKLR+ GRIWKEQ
Sbjct: 245 EGTPRSVRDSHRKVRDSHDGLIIQRNMALFFSGGDKKELKLRVTGRIWKEQ 294

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BLAST of CmaCh08G001100 vs. Swiss-Prot
Match: SINA4_ARATH (E3 ubiquitin-protein ligase SINAT4 OS=Arabidopsis thaliana GN=SINAT4 PE=2 SV=1)

HSP 1 Score: 470.3 bits (1209), Expect = 1.6e-131
Identity = 215/271 (79.34%), Postives = 239/271 (88.19%), Query Frame = 1


Query: 37  ISNNDNPT----TTSVHELLECPVCTNSMYPPIHQCPNGHTLCSSCKTKVDNRCPTCRQE 96
           ++N   PT     TSV+ELLECPVCT SMYPPIHQC NGHTLCS+CK +V NRCPTCRQE
Sbjct: 43  VTNIVGPTATAPATSVYELLECPVCTYSMYPPIHQCHNGHTLCSTCKVRVHNRCPTCRQE 102

Query: 97  LGDIRCLALEKIAESLKLACKFCTFGCSEILPYYSKLKHESACYFRPYTCPYAGSDCPVD 156
           LGDIRCLALEK+AESL+L CKF   GC EI PYYSKLKHES C FRPY+CPYAGS+C + 
Sbjct: 103 LGDIRCLALEKVAESLELPCKFYNLGCPEIFPYYSKLKHESLCNFRPYSCPYAGSECGIV 162

Query: 157 GEIPFLVSHLRDDHKVDMHSGCTFNHRYVKANPCEVENAIWMLTVFHCFDQYFCLHFEAF 216
           G+IPFLV+HLRDDHKVDMH+G TFNHRYVK+NP EVENA WMLTVFHCF QYFCLHFEAF
Sbjct: 163 GDIPFLVAHLRDDHKVDMHAGSTFNHRYVKSNPREVENATWMLTVFHCFGQYFCLHFEAF 222

Query: 217 QLGMAPVYMVFLRFMGDETDARNYSYSLEIGGNGRKLTWEGNPRSIRDNHKKVRDSHDGL 276
           QLGM PVYM FLRFMGDE DAR+YSYSLE+GG+GRKLTWEG PRSIRD+H+KVRDS+DGL
Sbjct: 223 QLGMGPVYMAFLRFMGDEEDARSYSYSLEVGGSGRKLTWEGTPRSIRDSHRKVRDSNDGL 282

Query: 277 IIHRNMALFFSGGERKELKLRIMGRIWKEQN 304
           II RNMALFFSGG+RKELKLR+ G+IWKEQ+
Sbjct: 283 IIQRNMALFFSGGDRKELKLRVTGKIWKEQH 313

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BLAST of CmaCh08G001100 vs. Swiss-Prot
Match: SINA2_ARATH (E3 ubiquitin-protein ligase SINAT2 OS=Arabidopsis thaliana GN=SINAT2 PE=1 SV=1)

HSP 1 Score: 439.9 bits (1130), Expect = 2.3e-122
Identity = 202/290 (69.66%), Postives = 235/290 (81.03%), Query Frame = 1


Query: 16  MDEEDKTLQCHCQFPSISKPR---ISNNDNPTTTSVHELLECPVCTNSMYPPIHQCPNGH 75
           MD E KT +        +KP    I      +   V+ELLECPVCTN MYPPIHQCPNGH
Sbjct: 19  MDYEVKTAKVEVNNNKPTKPGSAGIGKYGIHSNNGVYELLECPVCTNLMYPPIHQCPNGH 78

Query: 76  TLCSSCKTKVDNRCPTCRQELGDIRCLALEKIAESLKLACKFCTFGCSEILPYYSKLKHE 135
           TLCS+CK +V N CPTCR ELG+IRCLALEK+AESL++ C++   GC +I PYYSKLKHE
Sbjct: 79  TLCSNCKLRVQNTCPTCRYELGNIRCLALEKVAESLEVPCRYQNLGCHDIFPYYSKLKHE 138

Query: 136 SACYFRPYTCPYAGSDCPVDGEIPFLVSHLRDDHKVDMHSGCTFNHRYVKANPCEVENAI 195
             C FRPYTCPYAGS+C V G+IP LV HL+DDHKVDMH GCTFNHRYVK+NP EVENA 
Sbjct: 139 QHCRFRPYTCPYAGSECSVTGDIPTLVVHLKDDHKVDMHDGCTFNHRYVKSNPHEVENAT 198

Query: 196 WMLTVFHCFDQYFCLHFEAFQLGMAPVYMVFLRFMGDETDARNYSYSLEIGGNGRKLTWE 255
           WMLTVF+CF + FCLHFEAFQLGMAPVYM FLRFMGDE +A+ +SYSLE+G +GRKLTW+
Sbjct: 199 WMLTVFNCFGRQFCLHFEAFQLGMAPVYMAFLRFMGDENEAKKFSYSLEVGAHGRKLTWQ 258

Query: 256 GNPRSIRDNHKKVRDSHDGLIIHRNMALFFSGGERKELKLRIMGRIWKEQ 303
           G PRSIRD+H+KVRDS DGLII RN+AL+FSGG+R+ELKLR+ GRIWKE+
Sbjct: 259 GIPRSIRDSHRKVRDSQDGLIIPRNLALYFSGGDRQELKLRVTGRIWKEE 308

[Close]

BLAST of CmaCh08G001100 vs. Swiss-Prot
Match: SINA1_ARATH (Putative E3 ubiquitin-protein ligase SINAT1 OS=Arabidopsis thaliana GN=SINAT1 PE=3 SV=1)

HSP 1 Score: 431.0 bits (1107), Expect = 1.1e-119
Identity = 190/259 (73.36%), Postives = 223/259 (86.10%), Query Frame = 1


Query: 44  TTTSVHELLECPVCTNSMYPPIHQCPNGHTLCSSCKTKVDNRCPTCRQELGDIRCLALEK 103
           ++  V+ELLECPVCTN MYPPIHQCPNGHTLCSSCK +V N CPTCR ELG+IRCLALEK
Sbjct: 47  SSNGVYELLECPVCTNLMYPPIHQCPNGHTLCSSCKLRVQNTCPTCRYELGNIRCLALEK 106

Query: 104 IAESLKLACKFCTFGCSEILPYYSKLKHESACYFRPYTCPYAGSDCPVDGEIPFLVSHLR 163
           +AESL++ C++   GC +I PYYSKLKHE  C FR Y+CPYAGS+C V G+IP LV HL+
Sbjct: 107 VAESLEVPCRYQNLGCQDIFPYYSKLKHEQHCRFRSYSCPYAGSECSVTGDIPTLVDHLK 166

Query: 164 DDHKVDMHSGCTFNHRYVKANPCEVENAIWMLTVFHCFDQYFCLHFEAFQLGMAPVYMVF 223
           DDHKVDMH GCTFNHRYVK+NP EVENA WMLTVF+CF + FCLHFEAFQLGMAPVYM F
Sbjct: 167 DDHKVDMHDGCTFNHRYVKSNPHEVENATWMLTVFNCFGRQFCLHFEAFQLGMAPVYMAF 226

Query: 224 LRFMGDETDARNYSYSLEIGGNGRKLTWEGNPRSIRDNHKKVRDSHDGLIIHRNMALFFS 283
           LRFMGDE +A+ +SYSLE+G + RKLTW+G PRSIRD+H+KVRDS DGLII RN+AL+FS
Sbjct: 227 LRFMGDENEAKKFSYSLEVGAHSRKLTWQGIPRSIRDSHRKVRDSQDGLIIPRNLALYFS 286

Query: 284 GGERKELKLRIMGRIWKEQ 303
           G +++ELKLR+ GRIWKE+
Sbjct: 287 GSDKEELKLRVTGRIWKEE 305

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BLAST of CmaCh08G001100 vs. TrEMBL
Match: A0A0A0KF08_CUCSA (E3 ubiquitin-protein ligase OS=Cucumis sativus GN=Csa_6G087840 PE=3 SV=1)

HSP 1 Score: 614.0 bits (1582), Expect = 9.9e-173
Identity = 284/305 (93.11%), Postives = 291/305 (95.41%), Query Frame = 1


Query: 1   MELDGFERAPPSMDVMDEEDKTLQCHCQFPSISKPRISNNDNPTTTSVHELLECPVCTNS 60
           MELDG +   PSMDVMDEEDKTLQ HCQFPSISKPRI+NNDNPTTTSVHELLECPVCTNS
Sbjct: 1   MELDGID-GMPSMDVMDEEDKTLQRHCQFPSISKPRINNNDNPTTTSVHELLECPVCTNS 60

Query: 61  MYPPIHQCPNGHTLCSSCKTKVDNRCPTCRQELGDIRCLALEKIAESLKLACKFCTFGCS 120
           MYPPIHQCPNGHTLCSSCKT+VDNRCPTCRQELGDIRCLALEKIAESLKLACKF TFGC 
Sbjct: 61  MYPPIHQCPNGHTLCSSCKTRVDNRCPTCRQELGDIRCLALEKIAESLKLACKFSTFGCQ 120

Query: 121 EILPYYSKLKHESACYFRPYTCPYAGSDCPVDGEIPFLVSHLRDDHKVDMHSGCTFNHRY 180
           EILPYYSKLKHESACY+RPYTCPYAGSDCP+DG IPFLVSHLRDDHKVDMHSGCTFNHRY
Sbjct: 121 EILPYYSKLKHESACYYRPYTCPYAGSDCPIDGNIPFLVSHLRDDHKVDMHSGCTFNHRY 180

Query: 181 VKANPCEVENAIWMLTVFHCFDQYFCLHFEAFQLGMAPVYMVFLRFMGDETDARNYSYSL 240
           VKANPCEVENA WMLTVFHCF QYFCLHFEAFQLGMAPVYM FLRFMGDETDARN+SYSL
Sbjct: 181 VKANPCEVENATWMLTVFHCFGQYFCLHFEAFQLGMAPVYMAFLRFMGDETDARNFSYSL 240

Query: 241 EIGGNGRKLTWEGNPRSIRDNHKKVRDSHDGLIIHRNMALFFSGGERKELKLRIMGRIWK 300
           E+GGNGRKL WEGNPRSIRDNHKKVRDSHDGLII RNMALFFSGGERKELKLRI GRIWK
Sbjct: 241 EVGGNGRKLIWEGNPRSIRDNHKKVRDSHDGLIIQRNMALFFSGGERKELKLRITGRIWK 300

Query: 301 EQNKQ 306
           EQNKQ
Sbjct: 301 EQNKQ 304

[Close]

BLAST of CmaCh08G001100 vs. TrEMBL
Match: M5XLI4_PRUPE (E3 ubiquitin-protein ligase OS=Prunus persica GN=PRUPE_ppa008943mg PE=3 SV=1)

HSP 1 Score: 514.6 bits (1324), Expect = 8.2e-143
Identity = 238/302 (78.81%), Postives = 263/302 (87.09%), Query Frame = 1


Query: 1   MELDGFERAPPSMDVMDEEDKTLQCHCQFPSISKPRISNNDNPTTTSVHELLECPVCTNS 60
           ME DG E    S+D+MDE++   + H  F S+ KP  ++ + P TTSVHELLECPVCTNS
Sbjct: 1   MESDGIESVT-SLDMMDEDEIHGRPH-HFVSVPKPHNNSINIPGTTSVHELLECPVCTNS 60

Query: 61  MYPPIHQCPNGHTLCSSCKTKVDNRCPTCRQELGDIRCLALEKIAESLKLACKFCTFGCS 120
           MYPPIHQC NGHTLCS+CKT+V NRCPTCRQELGDIRCLALEK+AESL+L CK+C+ GC 
Sbjct: 61  MYPPIHQCHNGHTLCSTCKTRVHNRCPTCRQELGDIRCLALEKVAESLELPCKYCSLGCP 120

Query: 121 EILPYYSKLKHESACYFRPYTCPYAGSDCPVDGEIPFLVSHLRDDHKVDMHSGCTFNHRY 180
           EI PYYSKLKHE+ C FRPY CPYAGSDC   G+IPFLV+HLRDDHKVDMHSGCTFNHRY
Sbjct: 121 EIFPYYSKLKHEAVCNFRPYNCPYAGSDCSAVGDIPFLVTHLRDDHKVDMHSGCTFNHRY 180

Query: 181 VKANPCEVENAIWMLTVFHCFDQYFCLHFEAFQLGMAPVYMVFLRFMGDETDARNYSYSL 240
           VK+NP EVENA WMLTVFHCF QYFCLHFEAFQLGM PVYM FLRFMGDET+ARNY+YSL
Sbjct: 181 VKSNPREVENATWMLTVFHCFGQYFCLHFEAFQLGMGPVYMAFLRFMGDETEARNYTYSL 240

Query: 241 EIGGNGRKLTWEGNPRSIRDNHKKVRDSHDGLIIHRNMALFFSGGERKELKLRIMGRIWK 300
           E+GGNGRKL WEGNPRSIRD+HKKVRDSHDGLII RNMALFFSGG+RKELKLR+ GRIWK
Sbjct: 241 EVGGNGRKLIWEGNPRSIRDSHKKVRDSHDGLIIQRNMALFFSGGDRKELKLRVTGRIWK 300

Query: 301 EQ 303
           EQ
Sbjct: 301 EQ 300

[Close]

BLAST of CmaCh08G001100 vs. TrEMBL
Match: V4U1Z4_9ROSI (E3 ubiquitin-protein ligase OS=Citrus clementina GN=CICLE_v10021159mg PE=3 SV=1)

HSP 1 Score: 512.7 bits (1319), Expect = 3.1e-142
Identity = 241/311 (77.49%), Postives = 264/311 (84.89%), Query Frame = 1


Query: 1   MELDGFERAPPSMDVMDEEDKTLQCHCQFPSISKPRISNNDN---------PTTTSVHEL 60
           MEL   E   PS D+ DE++       QFPSISKP  + N+N         P TTSVHEL
Sbjct: 1   MELHSIE-CVPSSDLTDEDEIHHHRPHQFPSISKPHNNCNNNNTSLASVINPGTTSVHEL 60

Query: 61  LECPVCTNSMYPPIHQCPNGHTLCSSCKTKVDNRCPTCRQELGDIRCLALEKIAESLKLA 120
           LECPVCTNSMYPPIHQC NGHTLCS+CKT+V NRCPTCRQELGDIRCLALEK+AESL+L 
Sbjct: 61  LECPVCTNSMYPPIHQCHNGHTLCSTCKTRVHNRCPTCRQELGDIRCLALEKVAESLELP 120

Query: 121 CKFCTFGCSEILPYYSKLKHESACYFRPYTCPYAGSDCPVDGEIPFLVSHLRDDHKVDMH 180
           CK+ + GC EI PYYSKLKHE+ C FRPY CPYAGS+C + G+IPFLV+HLRDDHKVDMH
Sbjct: 121 CKYMSLGCPEIFPYYSKLKHEAICNFRPYNCPYAGSECSIVGDIPFLVAHLRDDHKVDMH 180

Query: 181 SGCTFNHRYVKANPCEVENAIWMLTVFHCFDQYFCLHFEAFQLGMAPVYMVFLRFMGDET 240
           SGCTFNHRYVK+NP EVENA WMLTVFHCF QYFCLHFEAFQLGMAPVYM FLRFMGDET
Sbjct: 181 SGCTFNHRYVKSNPHEVENATWMLTVFHCFGQYFCLHFEAFQLGMAPVYMAFLRFMGDET 240

Query: 241 DARNYSYSLEIGGNGRKLTWEGNPRSIRDNHKKVRDSHDGLIIHRNMALFFSGGERKELK 300
           +ARNY+YSLE+GGNGRKLTWEG PRSIRD+HKKVRDSHDGLII RNMALFFSGG+RKELK
Sbjct: 241 EARNYTYSLEVGGNGRKLTWEGTPRSIRDSHKKVRDSHDGLIIQRNMALFFSGGDRKELK 300

Query: 301 LRIMGRIWKEQ 303
           LR+ GRIWKEQ
Sbjct: 301 LRVTGRIWKEQ 310

[Close]

BLAST of CmaCh08G001100 vs. TrEMBL
Match: A0A067H6D5_CITSI (E3 ubiquitin-protein ligase OS=Citrus sinensis GN=CISIN_1g020592mg PE=3 SV=1)

HSP 1 Score: 512.7 bits (1319), Expect = 3.1e-142
Identity = 241/311 (77.49%), Postives = 264/311 (84.89%), Query Frame = 1


Query: 1   MELDGFERAPPSMDVMDEEDKTLQCHCQFPSISKPRISNNDN---------PTTTSVHEL 60
           MEL   E   PS D+ DE++       QFPSISKP  + N+N         P TTSVHEL
Sbjct: 1   MELHSIE-CVPSSDLTDEDEIHHHRPHQFPSISKPHNNCNNNNTSLASVINPGTTSVHEL 60

Query: 61  LECPVCTNSMYPPIHQCPNGHTLCSSCKTKVDNRCPTCRQELGDIRCLALEKIAESLKLA 120
           LECPVCTNSMYPPIHQC NGHTLCS+CKT+V NRCPTCRQELGDIRCLALEK+AESL+L 
Sbjct: 61  LECPVCTNSMYPPIHQCHNGHTLCSTCKTRVHNRCPTCRQELGDIRCLALEKVAESLELP 120

Query: 121 CKFCTFGCSEILPYYSKLKHESACYFRPYTCPYAGSDCPVDGEIPFLVSHLRDDHKVDMH 180
           CK+ + GC EI PYYSKLKHE+ C FRPY CPYAGS+C + G+IPFLV+HLRDDHKVDMH
Sbjct: 121 CKYMSLGCPEIFPYYSKLKHEAICNFRPYNCPYAGSECSIVGDIPFLVAHLRDDHKVDMH 180

Query: 181 SGCTFNHRYVKANPCEVENAIWMLTVFHCFDQYFCLHFEAFQLGMAPVYMVFLRFMGDET 240
           SGCTFNHRYVK+NP EVENA WMLTVFHCF QYFCLHFEAFQLGMAPVYM FLRFMGDET
Sbjct: 181 SGCTFNHRYVKSNPHEVENATWMLTVFHCFGQYFCLHFEAFQLGMAPVYMAFLRFMGDET 240

Query: 241 DARNYSYSLEIGGNGRKLTWEGNPRSIRDNHKKVRDSHDGLIIHRNMALFFSGGERKELK 300
           +ARNY+YSLE+GGNGRKLTWEG PRSIRD+HKKVRDSHDGLII RNMALFFSGG+RKELK
Sbjct: 241 EARNYTYSLEVGGNGRKLTWEGTPRSIRDSHKKVRDSHDGLIIQRNMALFFSGGDRKELK 300

Query: 301 LRIMGRIWKEQ 303
           LR+ GRIWKEQ
Sbjct: 301 LRVTGRIWKEQ 310

[Close]

BLAST of CmaCh08G001100 vs. TrEMBL
Match: A0A067K098_JATCU (E3 ubiquitin-protein ligase OS=Jatropha curcas GN=JCGZ_14254 PE=3 SV=1)

HSP 1 Score: 506.5 bits (1303), Expect = 2.2e-140
Identity = 241/319 (75.55%), Postives = 266/319 (83.39%), Query Frame = 1


Query: 1   MELDGFERAPPSMDVMDEEDKTLQCHCQFPSISKPR--------ISNNDNPTTT------ 60
           MELD  E  P S D+ DE D+    H QFPS+ KP+        I+NN+N  +       
Sbjct: 1   MELDCIETVPSS-DLTDE-DEIHHHHHQFPSVPKPQSNNININIINNNNNNNSNTVSSAI 60

Query: 61  ---SVHELLECPVCTNSMYPPIHQCPNGHTLCSSCKTKVDNRCPTCRQELGDIRCLALEK 120
              SVHELLECPVCTNSMYPPIHQC NGHTLCS+CKT+V NRCPTCRQELGDIRCLALEK
Sbjct: 61  QSISVHELLECPVCTNSMYPPIHQCHNGHTLCSTCKTRVHNRCPTCRQELGDIRCLALEK 120

Query: 121 IAESLKLACKFCTFGCSEILPYYSKLKHESACYFRPYTCPYAGSDCPVDGEIPFLVSHLR 180
           +AESL+L CK+ + GC EI PYYSKLKHE+ C FRPY+CPYAGS+C V G+IPFLV+HLR
Sbjct: 121 VAESLELPCKYMSLGCPEIFPYYSKLKHEALCNFRPYSCPYAGSECAVVGDIPFLVAHLR 180

Query: 181 DDHKVDMHSGCTFNHRYVKANPCEVENAIWMLTVFHCFDQYFCLHFEAFQLGMAPVYMVF 240
           DDHKVDMHSGCTFNHRYVK+NP EVENA WMLTVFHCF QYFCLHFEAFQLGMAPVYM F
Sbjct: 181 DDHKVDMHSGCTFNHRYVKSNPREVENATWMLTVFHCFGQYFCLHFEAFQLGMAPVYMAF 240

Query: 241 LRFMGDETDARNYSYSLEIGGNGRKLTWEGNPRSIRDNHKKVRDSHDGLIIHRNMALFFS 300
           LRFMGDET+ARNYSYSLE+GGNGRKL WEG PRSIRD+H+KVRDSHDGLII RNMALFFS
Sbjct: 241 LRFMGDETEARNYSYSLEVGGNGRKLVWEGTPRSIRDSHRKVRDSHDGLIIQRNMALFFS 300

Query: 301 GGERKELKLRIMGRIWKEQ 303
           GG+RKELKLR+ GRIWKEQ
Sbjct: 301 GGDRKELKLRVTGRIWKEQ 317

[Close]

BLAST of CmaCh08G001100 vs. TAIR10
Match: AT3G61790.1 (AT3G61790.1 Protein with RING/U-box and TRAF-like domains)

HSP 1 Score: 498.0 bits (1281), Expect = 4.0e-141
Identity = 233/315 (73.97%), Postives = 262/315 (83.17%), Query Frame = 1


Query: 1   MELDGFERAPPSMDVMDEEDKTLQCHCQFPSISKPRISNNDN-----------PTTTSVH 60
           M+LD  +    +MDV D+E+     H  + S+SK   +NN+            PTTTSVH
Sbjct: 1   MDLDSMD-CTSTMDVTDDEEIHQDRH-SYASVSKHHHTNNNTTNVNAAASGLLPTTTSVH 60

Query: 61  ELLECPVCTNSMYPPIHQCPNGHTLCSSCKTKVDNRCPTCRQELGDIRCLALEKIAESLK 120
           ELLECPVCTNSMYPPIHQC NGHTLCS+CK +V NRCPTCRQELGDIRCLALEK+AESL+
Sbjct: 61  ELLECPVCTNSMYPPIHQCHNGHTLCSTCKARVHNRCPTCRQELGDIRCLALEKVAESLE 120

Query: 121 LACKFCTFGCSEILPYYSKLKHESACYFRPYTCPYAGSDCPVDGEIPFLVSHLRDDHKVD 180
           L CK  + GC EI PYYSKLKHE+ C FRPY+CPYAGS+C V G+IPFLV+HLRDDHKVD
Sbjct: 121 LPCKHMSLGCPEIFPYYSKLKHETVCNFRPYSCPYAGSECSVTGDIPFLVAHLRDDHKVD 180

Query: 181 MHSGCTFNHRYVKANPCEVENAIWMLTVFHCFDQYFCLHFEAFQLGMAPVYMVFLRFMGD 240
           MHSGCTFNHRYVK+NP EVENA WMLTVFHCF QYFCLHFEAFQLGMAPVYM FLRFMGD
Sbjct: 181 MHSGCTFNHRYVKSNPREVENATWMLTVFHCFGQYFCLHFEAFQLGMAPVYMAFLRFMGD 240

Query: 241 ETDARNYSYSLEIGGNGRKLTWEGNPRSIRDNHKKVRDSHDGLIIHRNMALFFSGGERKE 300
           ET+ARNY+YSLE+GG GRKL WEG PRS+RD+H+KVRDSHDGLII RNMALFFSGG+RKE
Sbjct: 241 ETEARNYNYSLEVGGYGRKLIWEGTPRSVRDSHRKVRDSHDGLIIQRNMALFFSGGDRKE 300

Query: 301 LKLRIMGRIWKEQNK 305
           LKLR+ GRIWKEQ +
Sbjct: 301 LKLRVTGRIWKEQQQ 313

[Close]

BLAST of CmaCh08G001100 vs. TAIR10
Match: AT4G27880.1 (AT4G27880.1 Protein with RING/U-box and TRAF-like domains)

HSP 1 Score: 470.3 bits (1209), Expect = 8.9e-133
Identity = 215/271 (79.34%), Postives = 239/271 (88.19%), Query Frame = 1


Query: 37  ISNNDNPT----TTSVHELLECPVCTNSMYPPIHQCPNGHTLCSSCKTKVDNRCPTCRQE 96
           ++N   PT     TSV+ELLECPVCT SMYPPIHQC NGHTLCS+CK +V NRCPTCRQE
Sbjct: 43  VTNIVGPTATAPATSVYELLECPVCTYSMYPPIHQCHNGHTLCSTCKVRVHNRCPTCRQE 102

Query: 97  LGDIRCLALEKIAESLKLACKFCTFGCSEILPYYSKLKHESACYFRPYTCPYAGSDCPVD 156
           LGDIRCLALEK+AESL+L CKF   GC EI PYYSKLKHES C FRPY+CPYAGS+C + 
Sbjct: 103 LGDIRCLALEKVAESLELPCKFYNLGCPEIFPYYSKLKHESLCNFRPYSCPYAGSECGIV 162

Query: 157 GEIPFLVSHLRDDHKVDMHSGCTFNHRYVKANPCEVENAIWMLTVFHCFDQYFCLHFEAF 216
           G+IPFLV+HLRDDHKVDMH+G TFNHRYVK+NP EVENA WMLTVFHCF QYFCLHFEAF
Sbjct: 163 GDIPFLVAHLRDDHKVDMHAGSTFNHRYVKSNPREVENATWMLTVFHCFGQYFCLHFEAF 222

Query: 217 QLGMAPVYMVFLRFMGDETDARNYSYSLEIGGNGRKLTWEGNPRSIRDNHKKVRDSHDGL 276
           QLGM PVYM FLRFMGDE DAR+YSYSLE+GG+GRKLTWEG PRSIRD+H+KVRDS+DGL
Sbjct: 223 QLGMGPVYMAFLRFMGDEEDARSYSYSLEVGGSGRKLTWEGTPRSIRDSHRKVRDSNDGL 282

Query: 277 IIHRNMALFFSGGERKELKLRIMGRIWKEQN 304
           II RNMALFFSGG+RKELKLR+ G+IWKEQ+
Sbjct: 283 IIQRNMALFFSGGDRKELKLRVTGKIWKEQH 313

[Close]

BLAST of CmaCh08G001100 vs. TAIR10
Match: AT3G58040.1 (AT3G58040.1 seven in absentia of Arabidopsis 2)

HSP 1 Score: 439.9 bits (1130), Expect = 1.3e-123
Identity = 202/290 (69.66%), Postives = 235/290 (81.03%), Query Frame = 1


Query: 16  MDEEDKTLQCHCQFPSISKPR---ISNNDNPTTTSVHELLECPVCTNSMYPPIHQCPNGH 75
           MD E KT +        +KP    I      +   V+ELLECPVCTN MYPPIHQCPNGH
Sbjct: 19  MDYEVKTAKVEVNNNKPTKPGSAGIGKYGIHSNNGVYELLECPVCTNLMYPPIHQCPNGH 78

Query: 76  TLCSSCKTKVDNRCPTCRQELGDIRCLALEKIAESLKLACKFCTFGCSEILPYYSKLKHE 135
           TLCS+CK +V N CPTCR ELG+IRCLALEK+AESL++ C++   GC +I PYYSKLKHE
Sbjct: 79  TLCSNCKLRVQNTCPTCRYELGNIRCLALEKVAESLEVPCRYQNLGCHDIFPYYSKLKHE 138

Query: 136 SACYFRPYTCPYAGSDCPVDGEIPFLVSHLRDDHKVDMHSGCTFNHRYVKANPCEVENAI 195
             C FRPYTCPYAGS+C V G+IP LV HL+DDHKVDMH GCTFNHRYVK+NP EVENA 
Sbjct: 139 QHCRFRPYTCPYAGSECSVTGDIPTLVVHLKDDHKVDMHDGCTFNHRYVKSNPHEVENAT 198

Query: 196 WMLTVFHCFDQYFCLHFEAFQLGMAPVYMVFLRFMGDETDARNYSYSLEIGGNGRKLTWE 255
           WMLTVF+CF + FCLHFEAFQLGMAPVYM FLRFMGDE +A+ +SYSLE+G +GRKLTW+
Sbjct: 199 WMLTVFNCFGRQFCLHFEAFQLGMAPVYMAFLRFMGDENEAKKFSYSLEVGAHGRKLTWQ 258

Query: 256 GNPRSIRDNHKKVRDSHDGLIIHRNMALFFSGGERKELKLRIMGRIWKEQ 303
           G PRSIRD+H+KVRDS DGLII RN+AL+FSGG+R+ELKLR+ GRIWKE+
Sbjct: 259 GIPRSIRDSHRKVRDSQDGLIIPRNLALYFSGGDRQELKLRVTGRIWKEE 308

[Close]

BLAST of CmaCh08G001100 vs. TAIR10
Match: AT2G41980.1 (AT2G41980.1 Protein with RING/U-box and TRAF-like domains)

HSP 1 Score: 431.0 bits (1107), Expect = 6.0e-121
Identity = 190/259 (73.36%), Postives = 223/259 (86.10%), Query Frame = 1


Query: 44  TTTSVHELLECPVCTNSMYPPIHQCPNGHTLCSSCKTKVDNRCPTCRQELGDIRCLALEK 103
           ++  V+ELLECPVCTN MYPPIHQCPNGHTLCSSCK +V N CPTCR ELG+IRCLALEK
Sbjct: 47  SSNGVYELLECPVCTNLMYPPIHQCPNGHTLCSSCKLRVQNTCPTCRYELGNIRCLALEK 106

Query: 104 IAESLKLACKFCTFGCSEILPYYSKLKHESACYFRPYTCPYAGSDCPVDGEIPFLVSHLR 163
           +AESL++ C++   GC +I PYYSKLKHE  C FR Y+CPYAGS+C V G+IP LV HL+
Sbjct: 107 VAESLEVPCRYQNLGCQDIFPYYSKLKHEQHCRFRSYSCPYAGSECSVTGDIPTLVDHLK 166

Query: 164 DDHKVDMHSGCTFNHRYVKANPCEVENAIWMLTVFHCFDQYFCLHFEAFQLGMAPVYMVF 223
           DDHKVDMH GCTFNHRYVK+NP EVENA WMLTVF+CF + FCLHFEAFQLGMAPVYM F
Sbjct: 167 DDHKVDMHDGCTFNHRYVKSNPHEVENATWMLTVFNCFGRQFCLHFEAFQLGMAPVYMAF 226

Query: 224 LRFMGDETDARNYSYSLEIGGNGRKLTWEGNPRSIRDNHKKVRDSHDGLIIHRNMALFFS 283
           LRFMGDE +A+ +SYSLE+G + RKLTW+G PRSIRD+H+KVRDS DGLII RN+AL+FS
Sbjct: 227 LRFMGDENEAKKFSYSLEVGAHSRKLTWQGIPRSIRDSHRKVRDSQDGLIIPRNLALYFS 286

Query: 284 GGERKELKLRIMGRIWKEQ 303
           G +++ELKLR+ GRIWKE+
Sbjct: 287 GSDKEELKLRVTGRIWKEE 305

[Close]

BLAST of CmaCh08G001100 vs. TAIR10
Match: AT5G53360.1 (AT5G53360.1 TRAF-like superfamily protein)

HSP 1 Score: 360.1 bits (923), Expect = 1.3e-99
Identity = 162/204 (79.41%), Postives = 181/204 (88.73%), Query Frame = 1


Query: 99  LALEKIAESLKLACKFCTFGCSEILPYYSKLKHESACYFRPYTCPYAGSDCPVDGEIPFL 158
           +ALEK+AESL+L CK+   GC  I PYYSKLKHES C FRPY+CPYAGS+C   G+I FL
Sbjct: 15  IALEKVAESLELPCKYYNLGCLGIFPYYSKLKHESQCNFRPYSCPYAGSECAAVGDITFL 74

Query: 159 VSHLRDDHKVDMHSGCTFNHRYVKANPCEVENAIWMLTVFHCFDQYFCLHFEAFQLGMAP 218
           V+HLRDDHKVDMH+GCTFNHRYVK+NP EVENA WMLTVF CF QYFCLHFEAFQLGMAP
Sbjct: 75  VAHLRDDHKVDMHTGCTFNHRYVKSNPREVENATWMLTVFQCFGQYFCLHFEAFQLGMAP 134

Query: 219 VYMVFLRFMGDETDARNYSYSLEIGGNGRKLTWEGNPRSIRDNHKKVRDSHDGLIIHRNM 278
           VYM FLRFMGDE DARNY+YSLE+GG+GRK TWEG PRS+RD+H+KVRDSHDGLII RNM
Sbjct: 135 VYMAFLRFMGDEDDARNYTYSLEVGGSGRKQTWEGTPRSVRDSHRKVRDSHDGLIIQRNM 194

Query: 279 ALFFSGGERKELKLRIMGRIWKEQ 303
           ALFFSGG++KELKLR+ GRIWKEQ
Sbjct: 195 ALFFSGGDKKELKLRVTGRIWKEQ 218

[Close]

BLAST of CmaCh08G001100 vs. NCBI nr
Match: gi|449445654|ref|XP_004140587.1| (PREDICTED: E3 ubiquitin-protein ligase SINAT3 [Cucumis sativus])

HSP 1 Score: 614.0 bits (1582), Expect = 1.4e-172
Identity = 284/305 (93.11%), Postives = 291/305 (95.41%), Query Frame = 1


Query: 1   MELDGFERAPPSMDVMDEEDKTLQCHCQFPSISKPRISNNDNPTTTSVHELLECPVCTNS 60
           MELDG +   PSMDVMDEEDKTLQ HCQFPSISKPRI+NNDNPTTTSVHELLECPVCTNS
Sbjct: 1   MELDGID-GMPSMDVMDEEDKTLQRHCQFPSISKPRINNNDNPTTTSVHELLECPVCTNS 60

Query: 61  MYPPIHQCPNGHTLCSSCKTKVDNRCPTCRQELGDIRCLALEKIAESLKLACKFCTFGCS 120
           MYPPIHQCPNGHTLCSSCKT+VDNRCPTCRQELGDIRCLALEKIAESLKLACKF TFGC 
Sbjct: 61  MYPPIHQCPNGHTLCSSCKTRVDNRCPTCRQELGDIRCLALEKIAESLKLACKFSTFGCQ 120

Query: 121 EILPYYSKLKHESACYFRPYTCPYAGSDCPVDGEIPFLVSHLRDDHKVDMHSGCTFNHRY 180
           EILPYYSKLKHESACY+RPYTCPYAGSDCP+DG IPFLVSHLRDDHKVDMHSGCTFNHRY
Sbjct: 121 EILPYYSKLKHESACYYRPYTCPYAGSDCPIDGNIPFLVSHLRDDHKVDMHSGCTFNHRY 180

Query: 181 VKANPCEVENAIWMLTVFHCFDQYFCLHFEAFQLGMAPVYMVFLRFMGDETDARNYSYSL 240
           VKANPCEVENA WMLTVFHCF QYFCLHFEAFQLGMAPVYM FLRFMGDETDARN+SYSL
Sbjct: 181 VKANPCEVENATWMLTVFHCFGQYFCLHFEAFQLGMAPVYMAFLRFMGDETDARNFSYSL 240

Query: 241 EIGGNGRKLTWEGNPRSIRDNHKKVRDSHDGLIIHRNMALFFSGGERKELKLRIMGRIWK 300
           E+GGNGRKL WEGNPRSIRDNHKKVRDSHDGLII RNMALFFSGGERKELKLRI GRIWK
Sbjct: 241 EVGGNGRKLIWEGNPRSIRDNHKKVRDSHDGLIIQRNMALFFSGGERKELKLRITGRIWK 300

Query: 301 EQNKQ 306
           EQNKQ
Sbjct: 301 EQNKQ 304

[Close]

BLAST of CmaCh08G001100 vs. NCBI nr
Match: gi|659120058|ref|XP_008459990.1| (PREDICTED: E3 ubiquitin-protein ligase SINAT3-like [Cucumis melo])

HSP 1 Score: 612.5 bits (1578), Expect = 4.1e-172
Identity = 283/305 (92.79%), Postives = 291/305 (95.41%), Query Frame = 1


Query: 1   MELDGFERAPPSMDVMDEEDKTLQCHCQFPSISKPRISNNDNPTTTSVHELLECPVCTNS 60
           MELDG +   PSMDVMDEEDKTLQ HCQFPSISKPRI+NNDNPTTTSVHELLECPVCTNS
Sbjct: 1   MELDGID-GMPSMDVMDEEDKTLQRHCQFPSISKPRINNNDNPTTTSVHELLECPVCTNS 60

Query: 61  MYPPIHQCPNGHTLCSSCKTKVDNRCPTCRQELGDIRCLALEKIAESLKLACKFCTFGCS 120
           MYPPIHQCPNGHTLCSSCKT+VDNRCPTCRQELGDIRCLALEKIAESLKLACKF TFGC 
Sbjct: 61  MYPPIHQCPNGHTLCSSCKTRVDNRCPTCRQELGDIRCLALEKIAESLKLACKFSTFGCQ 120

Query: 121 EILPYYSKLKHESACYFRPYTCPYAGSDCPVDGEIPFLVSHLRDDHKVDMHSGCTFNHRY 180
           EILPYYSKLKHESACY+RPYTCPYAGSDCP+DG IPFLVSHLRDDHKVDMHSGCTFNHRY
Sbjct: 121 EILPYYSKLKHESACYYRPYTCPYAGSDCPIDGNIPFLVSHLRDDHKVDMHSGCTFNHRY 180

Query: 181 VKANPCEVENAIWMLTVFHCFDQYFCLHFEAFQLGMAPVYMVFLRFMGDETDARNYSYSL 240
           VKANPCEVENA WMLTVFHCF QYFCLHFEAFQLGMAPVYM FLRFMGDE+DARN+SYSL
Sbjct: 181 VKANPCEVENATWMLTVFHCFGQYFCLHFEAFQLGMAPVYMAFLRFMGDESDARNFSYSL 240

Query: 241 EIGGNGRKLTWEGNPRSIRDNHKKVRDSHDGLIIHRNMALFFSGGERKELKLRIMGRIWK 300
           E+GGNGRKL WEGNPRSIRDNHKKVRDSHDGLII RNMALFFSGGERKELKLRI GRIWK
Sbjct: 241 EVGGNGRKLIWEGNPRSIRDNHKKVRDSHDGLIIQRNMALFFSGGERKELKLRITGRIWK 300

Query: 301 EQNKQ 306
           EQNKQ
Sbjct: 301 EQNKQ 304

[Close]

BLAST of CmaCh08G001100 vs. NCBI nr
Match: gi|1009142384|ref|XP_015888695.1| (PREDICTED: E3 ubiquitin-protein ligase SINAT3-like [Ziziphus jujuba])

HSP 1 Score: 523.1 bits (1346), Expect = 3.3e-145
Identity = 245/302 (81.13%), Postives = 264/302 (87.42%), Query Frame = 1


Query: 1   MELDGFERAPPSMDVMDEEDKTLQCHCQFPSISKPRISNNDNPTTTSVHELLECPVCTNS 60
           ME D  E  PPS DVMDE D+      QF ++SKP  + N N +TTSVHELLECPVCTNS
Sbjct: 1   MESDCIETVPPS-DVMDE-DEIQGRPLQFLTVSKPLSNTNSNSSTTSVHELLECPVCTNS 60

Query: 61  MYPPIHQCPNGHTLCSSCKTKVDNRCPTCRQELGDIRCLALEKIAESLKLACKFCTFGCS 120
           MYPPIHQC NGHTLCS+CKT V NRCPTCRQELGDIRCLALEK+AESL+L CK+C+ GCS
Sbjct: 61  MYPPIHQCHNGHTLCSTCKTMVHNRCPTCRQELGDIRCLALEKVAESLELPCKYCSLGCS 120

Query: 121 EILPYYSKLKHESACYFRPYTCPYAGSDCPVDGEIPFLVSHLRDDHKVDMHSGCTFNHRY 180
           EI PYYSKLKHES C FRPY CPYAGSDC V G+IPFLV+HLRDDHKVDMHSGCTFNHRY
Sbjct: 121 EIFPYYSKLKHESLCNFRPYNCPYAGSDCSVVGDIPFLVAHLRDDHKVDMHSGCTFNHRY 180

Query: 181 VKANPCEVENAIWMLTVFHCFDQYFCLHFEAFQLGMAPVYMVFLRFMGDETDARNYSYSL 240
           VK+NP EVENA WMLTVFHCF QYFCLHFEAFQL MAPVYM FLRFMGDET+ARNYSYSL
Sbjct: 181 VKSNPREVENATWMLTVFHCFGQYFCLHFEAFQLEMAPVYMAFLRFMGDETEARNYSYSL 240

Query: 241 EIGGNGRKLTWEGNPRSIRDNHKKVRDSHDGLIIHRNMALFFSGGERKELKLRIMGRIWK 300
           E+GGNGRKL WEGNPRSIRD+H+KVRDSHDGLII RNMALFFSGG+RKELKLR+ GRIWK
Sbjct: 241 EVGGNGRKLIWEGNPRSIRDSHRKVRDSHDGLIIQRNMALFFSGGDRKELKLRVTGRIWK 300

Query: 301 EQ 303
           EQ
Sbjct: 301 EQ 300

[Close]

BLAST of CmaCh08G001100 vs. NCBI nr
Match: gi|470141909|ref|XP_004306667.1| (PREDICTED: E3 ubiquitin-protein ligase SINAT3 [Fragaria vesca subsp. vesca])

HSP 1 Score: 518.8 bits (1335), Expect = 6.2e-144
Identity = 239/302 (79.14%), Postives = 266/302 (88.08%), Query Frame = 1


Query: 1   MELDGFERAPPSMDVMDEEDKTLQCHCQFPSISKPRISNNDNPTTTSVHELLECPVCTNS 60
           M+ +  E  PPS D+MDE++   +    F ++ KPR + N+NP TTSVHELLECPVCTNS
Sbjct: 1   MDPENMEIVPPS-DLMDEDEIHGRPRQLF-TVPKPRNNGNNNPGTTSVHELLECPVCTNS 60

Query: 61  MYPPIHQCPNGHTLCSSCKTKVDNRCPTCRQELGDIRCLALEKIAESLKLACKFCTFGCS 120
           MYPPIHQC NGHTLCS+CKT+V NRCPTCRQELGDIRCLALEK+AESL+L CK+C+ GC 
Sbjct: 61  MYPPIHQCHNGHTLCSTCKTRVHNRCPTCRQELGDIRCLALEKVAESLELPCKYCSLGCP 120

Query: 121 EILPYYSKLKHESACYFRPYTCPYAGSDCPVDGEIPFLVSHLRDDHKVDMHSGCTFNHRY 180
           EI PYYSKLKHE+ C FRPY CPYAGSDC V G+IPFLV+HLRDDHKVDMHSGCTFNHRY
Sbjct: 121 EIFPYYSKLKHEAVCNFRPYNCPYAGSDCSVVGDIPFLVAHLRDDHKVDMHSGCTFNHRY 180

Query: 181 VKANPCEVENAIWMLTVFHCFDQYFCLHFEAFQLGMAPVYMVFLRFMGDETDARNYSYSL 240
           VK+NP EVENA WMLTVFHCF QYFCLHFEAFQLGMAPVYM FLRFMGDET+ARNY+YSL
Sbjct: 181 VKSNPREVENATWMLTVFHCFGQYFCLHFEAFQLGMAPVYMAFLRFMGDETEARNYTYSL 240

Query: 241 EIGGNGRKLTWEGNPRSIRDNHKKVRDSHDGLIIHRNMALFFSGGERKELKLRIMGRIWK 300
           E+GGNGRK+ WEGNPRSIRD+HKKVRDSHDGLII RNMALFFSGG+RKELKLR+ GRIWK
Sbjct: 241 EVGGNGRKIIWEGNPRSIRDSHKKVRDSHDGLIIQRNMALFFSGGDRKELKLRVTGRIWK 300

Query: 301 EQ 303
           EQ
Sbjct: 301 EQ 300

[Close]

BLAST of CmaCh08G001100 vs. NCBI nr
Match: gi|645254668|ref|XP_008233143.1| (PREDICTED: E3 ubiquitin-protein ligase SINAT3 isoform X2 [Prunus mume])

HSP 1 Score: 514.6 bits (1324), Expect = 1.2e-142
Identity = 238/302 (78.81%), Postives = 263/302 (87.09%), Query Frame = 1


Query: 1   MELDGFERAPPSMDVMDEEDKTLQCHCQFPSISKPRISNNDNPTTTSVHELLECPVCTNS 60
           ME DG E    S+D+MDE++   + H  F S+ KP  ++ + P TTSVHELLECPVCTNS
Sbjct: 1   MESDGIESVT-SLDLMDEDEIHGRPH-HFVSVPKPHNNSINIPGTTSVHELLECPVCTNS 60

Query: 61  MYPPIHQCPNGHTLCSSCKTKVDNRCPTCRQELGDIRCLALEKIAESLKLACKFCTFGCS 120
           MYPPIHQC NGHTLCS+CKT+V NRCPTCRQELGDIRCLALEK+AESL+L CK+C+ GC 
Sbjct: 61  MYPPIHQCHNGHTLCSTCKTRVHNRCPTCRQELGDIRCLALEKVAESLELPCKYCSLGCP 120

Query: 121 EILPYYSKLKHESACYFRPYTCPYAGSDCPVDGEIPFLVSHLRDDHKVDMHSGCTFNHRY 180
           EI PYYSKLKHE+ C FRPY CPYAGSDC   G+IPFLV+HLRDDHKVDMHSGCTFNHRY
Sbjct: 121 EIFPYYSKLKHEAVCNFRPYNCPYAGSDCSAVGDIPFLVTHLRDDHKVDMHSGCTFNHRY 180

Query: 181 VKANPCEVENAIWMLTVFHCFDQYFCLHFEAFQLGMAPVYMVFLRFMGDETDARNYSYSL 240
           VK+NP EVENA WMLTVFHCF QYFCLHFEAFQLGM PVYM FLRFMGDET+ARNY+YSL
Sbjct: 181 VKSNPREVENATWMLTVFHCFGQYFCLHFEAFQLGMGPVYMAFLRFMGDETEARNYTYSL 240

Query: 241 EIGGNGRKLTWEGNPRSIRDNHKKVRDSHDGLIIHRNMALFFSGGERKELKLRIMGRIWK 300
           E+GGNGRKL WEGNPRSIRD+HKKVRDSHDGLII RNMALFFSGG+RKELKLR+ GRIWK
Sbjct: 241 EVGGNGRKLIWEGNPRSIRDSHKKVRDSHDGLIIQRNMALFFSGGDRKELKLRVTGRIWK 300

Query: 301 EQ 303
           EQ
Sbjct: 301 EQ 300

The following BLAST results are available for this feature:

BLAST of CmaCh08G001100 vs. Swiss-Prot


Match Name	E-value	Identity	Description
SINA3_ARATH	7.1e-140	73.97	E3 ubiquitin-protein ligase SINAT3 OS=Arabidopsis thaliana GN=SINAT3 PE=2 SV=1	[more]
SINA5_ARATH	1.2e-131	74.91	E3 ubiquitin-protein ligase SINAT5 OS=Arabidopsis thaliana GN=SINAT5 PE=1 SV=2	[more]
SINA4_ARATH	1.6e-131	79.34	E3 ubiquitin-protein ligase SINAT4 OS=Arabidopsis thaliana GN=SINAT4 PE=2 SV=1	[more]
SINA2_ARATH	2.3e-122	69.66	E3 ubiquitin-protein ligase SINAT2 OS=Arabidopsis thaliana GN=SINAT2 PE=1 SV=1	[more]
SINA1_ARATH	1.1e-119	73.36	Putative E3 ubiquitin-protein ligase SINAT1 OS=Arabidopsis thaliana GN=SINAT1 PE...	[more]

BLAST of CmaCh08G001100 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KF08_CUCSA	9.9e-173	93.11	E3 ubiquitin-protein ligase OS=Cucumis sativus GN=Csa_6G087840 PE=3 SV=1	[more]
M5XLI4_PRUPE	8.2e-143	78.81	E3 ubiquitin-protein ligase OS=Prunus persica GN=PRUPE_ppa008943mg PE=3 SV=1	[more]
V4U1Z4_9ROSI	3.1e-142	77.49	E3 ubiquitin-protein ligase OS=Citrus clementina GN=CICLE_v10021159mg PE=3 SV=1	[more]
A0A067H6D5_CITSI	3.1e-142	77.49	E3 ubiquitin-protein ligase OS=Citrus sinensis GN=CISIN_1g020592mg PE=3 SV=1	[more]
A0A067K098_JATCU	2.2e-140	75.55	E3 ubiquitin-protein ligase OS=Jatropha curcas GN=JCGZ_14254 PE=3 SV=1	[more]

BLAST of CmaCh08G001100 vs. TAIR10


Match Name	E-value	Identity	Description
AT3G61790.1	4.0e-141	73.97	Protein with RING/U-box and TRAF-like domains	[more]
AT4G27880.1	8.9e-133	79.34	Protein with RING/U-box and TRAF-like domains	[more]
AT3G58040.1	1.3e-123	69.66	seven in absentia of Arabidopsis 2	[more]
AT2G41980.1	6.0e-121	73.36	Protein with RING/U-box and TRAF-like domains	[more]
AT5G53360.1	1.3e-99	79.41	TRAF-like superfamily protein	[more]

BLAST of CmaCh08G001100 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|449445654\|ref\|XP_004140587.1\|	1.4e-172	93.11	PREDICTED: E3 ubiquitin-protein ligase SINAT3 [Cucumis sativus]	[more]
gi\|659120058\|ref\|XP_008459990.1\|	4.1e-172	92.79	PREDICTED: E3 ubiquitin-protein ligase SINAT3-like [Cucumis melo]	[more]
gi\|1009142384\|ref\|XP_015888695.1\|	3.3e-145	81.13	PREDICTED: E3 ubiquitin-protein ligase SINAT3-like [Ziziphus jujuba]	[more]
gi\|470141909\|ref\|XP_004306667.1\|	6.2e-144	79.14	PREDICTED: E3 ubiquitin-protein ligase SINAT3 [Fragaria vesca subsp. vesca]	[more]
gi\|645254668\|ref\|XP_008233143.1\|	1.2e-142	78.81	PREDICTED: E3 ubiquitin-protein ligase SINAT3 isoform X2 [Prunus mume]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR001841	Znf_RING
IPR004162	SINA-like
IPR008974	TRAF-like
IPR013010	Znf_SIAH
IPR013083	Znf_RING/FYVE/PHD
IPR013323	SIAH-type domain
IPR018121	7-in-absentia-prot_TRAF-dom

Vocabulary: **Molecular Function**
Term	Definition
GO:0005515	protein binding
GO:0008270	zinc ion binding
GO:0004842	ubiquitin-protein transferase activity

Vocabulary: **Cellular Component**
Term	Definition
GO:0005634	nucleus

Vocabulary: **Biological Process**
Term	Definition
GO:0006511	ubiquitin-dependent protein catabolic process
GO:0007275	multicellular organism development
GO:0016567	protein ubiquitination

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0007275	multicellular organism development
biological_process	GO:0016567	protein ubiquitination
biological_process	GO:0006511	ubiquitin-dependent protein catabolic process
cellular_component	GO:0005634	nucleus
molecular_function	GO:0016874	ligase activity
molecular_function	GO:0005515	protein binding
molecular_function	GO:0004842	ubiquitin-protein transferase activity
molecular_function	GO:0008270	zinc ion binding

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmaCh08G001100.1	CmaCh08G001100.1	mRNA

InterPro

Analysis Name: InterPro Annotations of Cucurbita maxima
Date Performed: 2017-05-20


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001841	Zinc finger, RING-type	PROFILE	PS50089	ZF_RING_2	coord: 54..90 score: 9
IPR004162	E3 ubiquitin-protein ligase SIN-like	PANTHER	PTHR10315	SEVEN IN ABSENTIA HOMOLOG	coord: 12..305 score: 2.9E
IPR008974	TRAF-like	GENE3D	G3DSA:2.60.210.10		coord: 172..299 score: 2.4
IPR008974	TRAF-like	unknown	SSF49599	TRAF domain-like	coord: 107..298 score: 6.34
IPR013010	Zinc finger, SIAH-type	PROFILE	PS51081	ZF_SIAH	coord: 107..167 score: 1
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	G3DSA:3.30.40.10		coord: 40..119 score: 7.2
IPR013323	SIAH-type domain	GENE3D	G3DSA:3.90.890.10		coord: 120..162 score: 2.3
IPR018121	Seven-in-absentia protein, TRAF-like domain	PFAM	PF03145	Sina	coord: 96..294 score: 1.5
None	No IPR available	PANTHER	PTHR10315:SF34	E3 UBIQUITIN-PROTEIN LIGASE SINAT3-RELATED	coord: 12..305 score: 2.9E
None	No IPR available	unknown	SSF57850	RING/U-box	coord: 48..94 score: 2.6

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
CmaCh08G001100	CmoCh17G005390	Cucurbita moschata (Rifu)	cmacmoB895
CmaCh08G001100	CmoCh08G001160	Cucurbita moschata (Rifu)	cmacmoB913
CmaCh08G001100	Cp4.1LG12g04730	Cucurbita pepo (Zucchini)	cmacpeB901
CmaCh08G001100	Cp4.1LG17g10090	Cucurbita pepo (Zucchini)	cmacpeB914
CmaCh08G001100	Carg04939	Silver-seed gourd	carcmaB0112

The following gene(s) are paralogous to this gene:


Gene	Paralogue	Organism	Block
CmaCh08G001100	CmaCh17G005590	Cucurbita maxima (Rimu)	cmacmaB381

The following block(s) are covering this gene:


Gene	Organism	Block
CmaCh08G001100	Cucurbita maxima (Rimu)	cmacmaB386
CmaCh08G001100	Cucurbita moschata (Rifu)	cmacmoB882
CmaCh08G001100	Cucurbita moschata (Rifu)	cmacmoB900
CmaCh08G001100	Wild cucumber (PI 183967)	cmacpiB932
CmaCh08G001100	Cucumber (Chinese Long) v2	cmacuB911
CmaCh08G001100	Melon (DHL92) v3.5.1	cmameB819
CmaCh08G001100	Watermelon (Charleston Gray)	cmawcgB807
CmaCh08G001100	Watermelon (97103) v1	cmawmB876
CmaCh08G001100	Cucurbita pepo (Zucchini)	cmacpeB906
CmaCh08G001100	Bottle gourd (USVL1VR-Ls)	cmalsiB814
CmaCh08G001100	Bottle gourd (USVL1VR-Ls)	cmalsiB838
CmaCh08G001100	Cucumber (Gy14) v2	cgybcmaB464
CmaCh08G001100	Melon (DHL92) v3.6.1	cmamedB917
CmaCh08G001100	Melon (DHL92) v3.6.1	cmamedB955
CmaCh08G001100	Silver-seed gourd	carcmaB0305
CmaCh08G001100	Cucumber (Chinese Long) v3	cmacucB1061
CmaCh08G001100	Cucumber (Chinese Long) v3	cmacucB1083
CmaCh08G001100	Watermelon (97103) v2	cmawmbB924
CmaCh08G001100	Wax gourd	cmawgoB1094
CmaCh08G001100	Wax gourd	cmawgoB1115