CmaCh04G018130 (gene) Cucurbita maxima (Rimu)

NameCmaCh04G018130
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu))
DescriptionMitotic spindle assembly checkpoint protein MAD2
LocationCma_Chr04 : 9131708 .. 9133857 (-)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CTCGTCCTGCGATTCGGAGTTCTCAAATTTCCTCAGATCCACTCGTTTTTCTCTGTCAATTTGATTACTCGATCGATTTCACTTTCCAATTTGGTTACCCCTGCATCGATTAGAAGCCCTAAATCCGATCCTCGTCTTAGAATCGAAGAAATTCGGGAACAATTCTTATTGTTTCAGAAGATTTGGTGGAATGGCTTCGAAAACAGCGTCCAAAGACATCATCACTCTTCGCGGTTCTGCAGCGATTGTCAGCGAGTTCTTCGGTATATATTGCTTTAGTGCCTTATGAAATCCTTTTTATCTCGATTTTCTGTTTACTTCTGGCGATTATCGATGAATATCCTTGGCTGATTAATTCTTTTTCGTTGTCCTGAAATTTATCATTTAGGGTATGCTGCTAATAGGTATGAATTATGGATGCGCTTTCTCTGTTCTTATATTTGTTTTGCGATCATGTAAATTTGCATTTTGTCGCTTACAAGCGATGTCGATTGTAAATGTGTAGCATCTTGTACAATCGTGGACTTTATCCTGAAGAAAGTTTTGCAAGGGTGAAGAAATATGGACTTCCAATGTTGCTTACTCAAGATGAAGGTGTTAAGACCTTCATTTCTAATCTTACTGCTCAGCTTTCTGGTAAGTTCCCCTTCTCTAAATGAAAGATTTGTATGCTTTTTTTTACTTTCCTGAGAAGTGTATTCATTGAGAAGTTTTTGAATCGTGAAATTGGTTTTAGAATGGCTGGAAGCTGGGAAGCTGCAAAGGGTTGTGCTGGTTATAATGAGCAAAGCCAACAATGAGGTGCTTGAGAGGTGGAATTTCAGAATTGAAACTGATGGAGTGGTGGTCGAGCAAGGGTAAGAAATTCATGGAAAACCTTCTCTTCCCTTGCATAGATTTCTGGATTTGGTGTTTGATTAGATAAACATTGTTGCAGTGTGTCGAGGGAAAAGAGTGATAAAGAAATAATGAGGGAGATCCAAGCAATTATGAGACAGATTGCTTCGAGCATCACTTACTTGCCCTGCCTAGATGAACCCTGTAAGTGTTGCCAAAATTATCATAATCCATCATTTATTGATTTTGGGATTGGTGTAACATAATGTGATATTGTTAAGTCGGATTCTGAATCCATGGGATGTCCTGAAGTCATTATGCAGAACCTTGCAAGCGATGCTTCAAGTATGTGTCACTCTCTTGTTCAGCCTAGATGATGAACCCTTTATGTGTTGGCAAAATTACCACGATGTGCCATATAATTTAACATACCATCATGAAATCAATACTAGCCAACTAGAACATAGCTTGAATGGTTGGGCATGTGTCTCTTTCTTATGGGACTTGAGTTCTAATCCCTTGTTTGCATTCTAAAAGGTAATCATAGCTAATTATAATGATTCGTGAGTGATTTAAAAAGTAATCTAGCTCTTTGATTTTTGGACGATATTGATGATCCATGGTACTCAATCAGGCGCTTTCTTGACGTTTTTGCTGTGTCAGGTGTATTTGATGTGTTAGCATATACAGATAAAGATCTTGCAGTTCCATTTACTTGGATCGAGAGTGACCCTAAGCTGATTGCTAATCCACAAATGGTGAAGCTGCACTCATTTGATACAAAGGTGAGGGAAAGAGACTCCGTGGAGGAATATCAAAACTTATTTGTTAAGCTAACTAACTTTTAAACGGTGTTATTGCAGATTCACAAAGTGGACACTCTTGTTTCTTACAAGAACGATGACTGCGACGACGAGTAGAAAACAAACTCGCTTTCCTGGAATTTGTTGTTGGGGTTGTGGTGGGATCCCTCCAACCCGATCGTTTTCAAGGTGGCATTTTGTGAATCTTTTATTCACATTTGGTGTTGTACTGTAATGTAATGTGTAATGTAGTAACAATGTTTTGTGGGAGTGAGTGGTGAATATAGGGCAGGATGGGTTCACTTAGAATTCCACCTGTGAATGGTTTTGGGTTTGTTTGTTTGTTGTTGTGTGTGGTTGGGGGAACTTTTGAAGACCATATGACCTTGACCACCTTCTTTATAAAGAAAACATGTTTTACGGACAGGTTTTATCTTAAGAACAAGGGTGGACCCAAGTCAGACTTGTTCAAACATATTCATAGTAAGAGAGGTTCAAATCACAATTCCA

mRNA sequence

CTCGTCCTGCGATTCGGAGTTCTCAAATTTCCTCAGATCCACTCGTTTTTCTCTGTCAATTTGATTACTCGATCGATTTCACTTTCCAATTTGGTTACCCCTGCATCGATTAGAAGCCCTAAATCCGATCCTCGTCTTAGAATCGAAGAAATTCGGGAACAATTCTTATTGTTTCAGAAGATTTGGTGGAATGGCTTCGAAAACAGCGTCCAAAGACATCATCACTCTTCGCGGTTCTGCAGCGATTGTCAGCGAGTTCTTCGGGTATGCTGCTAATAGCATCTTGTACAATCGTGGACTTTATCCTGAAGAAAGTTTTGCAAGGGTGAAGAAATATGGACTTCCAATGTTGCTTACTCAAGATGAAGGTGTTAAGACCTTCATTTCTAATCTTACTGCTCAGCTTTCTGAATGGCTGGAAGCTGGGAAGCTGCAAAGGGTTGTGCTGGTTATAATGAGCAAAGCCAACAATGAGGTGCTTGAGAGGTGGAATTTCAGAATTGAAACTGATGGAGTGGTGGTCGAGCAAGGTGTGTCGAGGGAAAAGAGTGATAAAGAAATAATGAGGGAGATCCAAGCAATTATGAGACAGATTGCTTCGAGCATCACTTACTTGCCCTGCCTAGATGAACCCTGTGTATTTGATGTGTTAGCATATACAGATAAAGATCTTGCAGTTCCATTTACTTGGATCGAGAGTGACCCTAAGCTGATTGCTAATCCACAAATGGTGAAGCTGCACTCATTTGATACAAAGATTCACAAAGTGGACACTCTTGTTTCTTACAAGAACGATGACTGCGACGACGAGTAGAAAACAAACTCGCTTTCCTGGAATTTGTTGTTGGGGTTGTGGTGGGATCCCTCCAACCCGATCGTTTTCAAGGTGGCATTTTGTGAATCTTTTATTCACATTTGGTGTTGTACTGTAATGTAATGTGTAATGTAGTAACAATGTTTTGTGGGAGTGAGTGGTGAATATAGGGCAGGATGGGTTCACTTAGAATTCCACCTGTGAATGGTTTTGGGTTTGTTTGTTTGTTGTTGTGTGTGGTTGGGGGAACTTTTGAAGACCATATGACCTTGACCACCTTCTTTATAAAGAAAACATGTTTTACGGACAGGTTTTATCTTAAGAACAAGGGTGGACCCAAGTCAGACTTGTTCAAACATATTCATAGTAAGAGAGGTTCAAATCACAATTCCA

Coding sequence (CDS)

ATGGCTTCGAAAACAGCGTCCAAAGACATCATCACTCTTCGCGGTTCTGCAGCGATTGTCAGCGAGTTCTTCGGGTATGCTGCTAATAGCATCTTGTACAATCGTGGACTTTATCCTGAAGAAAGTTTTGCAAGGGTGAAGAAATATGGACTTCCAATGTTGCTTACTCAAGATGAAGGTGTTAAGACCTTCATTTCTAATCTTACTGCTCAGCTTTCTGAATGGCTGGAAGCTGGGAAGCTGCAAAGGGTTGTGCTGGTTATAATGAGCAAAGCCAACAATGAGGTGCTTGAGAGGTGGAATTTCAGAATTGAAACTGATGGAGTGGTGGTCGAGCAAGGTGTGTCGAGGGAAAAGAGTGATAAAGAAATAATGAGGGAGATCCAAGCAATTATGAGACAGATTGCTTCGAGCATCACTTACTTGCCCTGCCTAGATGAACCCTGTGTATTTGATGTGTTAGCATATACAGATAAAGATCTTGCAGTTCCATTTACTTGGATCGAGAGTGACCCTAAGCTGATTGCTAATCCACAAATGGTGAAGCTGCACTCATTTGATACAAAGATTCACAAAGTGGACACTCTTGTTTCTTACAAGAACGATGACTGCGACGACGAGTAG

Protein sequence

MASKTASKDIITLRGSAAIVSEFFGYAANSILYNRGLYPEESFARVKKYGLPMLLTQDEGVKTFISNLTAQLSEWLEAGKLQRVVLVIMSKANNEVLERWNFRIETDGVVVEQGVSREKSDKEIMREIQAIMRQIASSITYLPCLDEPCVFDVLAYTDKDLAVPFTWIESDPKLIANPQMVKLHSFDTKIHKVDTLVSYKNDDCDDE
BLAST of CmaCh04G018130 vs. Swiss-Prot
Match: MAD2_ARATH (Mitotic spindle checkpoint protein MAD2 OS=Arabidopsis thaliana GN=MAD2 PE=1 SV=1)

HSP 1 Score: 353.2 bits (905), Expect = 1.9e-96
Identity = 178/208 (85.58%), Postives = 193/208 (92.79%), Query Frame = 1

Query: 1   MASKTAS-KDIITLRGSAAIVSEFFGYAANSILYNRGLYPEESFARVKKYGLPMLLTQDE 60
           MASKTA+ KDIITL GSAAIVSEFF YAANSILYNR +YPEESF +VKKYGLPMLL +DE
Sbjct: 1   MASKTAAAKDIITLHGSAAIVSEFFCYAANSILYNRAVYPEESFVKVKKYGLPMLLIEDE 60

Query: 61  GVKTFISNLTAQLSEWLEAGKLQRVVLVIMSKANNEVLERWNFRIETDGVVVEQGVSREK 120
            VK+F+SNLT+Q+SEWLEAGKLQRVVLVIMSKA  EVLERWNFRIETD  VV++GVSREK
Sbjct: 61  SVKSFMSNLTSQISEWLEAGKLQRVVLVIMSKATGEVLERWNFRIETDNEVVDKGVSREK 120

Query: 121 SDKEIMREIQAIMRQIASSITYLPCLDEPCVFDVLAYTDKDLAVPFTWIESDPKLIANPQ 180
           SDKEIMREIQAIMRQ+ASS+TYLPCLDE CVFDVLAYTD D+AVPFTWIESDPKLIANPQ
Sbjct: 121 SDKEIMREIQAIMRQVASSVTYLPCLDETCVFDVLAYTDTDVAVPFTWIESDPKLIANPQ 180

Query: 181 MVKLHSFDTKIHKVDTLVSYKNDDCDDE 208
           MVKLH FDTKIHKVDTLVSYKND+ D+E
Sbjct: 181 MVKLHGFDTKIHKVDTLVSYKNDEWDEE 208

BLAST of CmaCh04G018130 vs. Swiss-Prot
Match: MAD2_MAIZE (Mitotic spindle checkpoint protein MAD2 OS=Zea mays GN=MAD2 PE=2 SV=1)

HSP 1 Score: 342.8 bits (878), Expect = 2.6e-93
Identity = 172/207 (83.09%), Postives = 188/207 (90.82%), Query Frame = 1

Query: 1   MASKTASKDIITLRGSAAIVSEFFGYAANSILYNRGLYPEESFARVKKYGLPMLLTQDEG 60
           MAS++ASKDIITLRGSAAIVSEFFGYAANSILYNR +YPEESF++VKKYGL MLLTQDEG
Sbjct: 1   MASRSASKDIITLRGSAAIVSEFFGYAANSILYNRAVYPEESFSKVKKYGLTMLLTQDEG 60

Query: 61  VKTFISNLTAQLSEWLEAGKLQRVVLVIMSKANNEVLERWNFRIETDGVVVEQGVSREKS 120
           VK FI++LT+QLSEWLEAGKLQR+VLVIMSKA +EVLERWNF I TD  VVE+G  +EKS
Sbjct: 61  VKNFIASLTSQLSEWLEAGKLQRIVLVIMSKATSEVLERWNFNIVTDAEVVEKGAIKEKS 120

Query: 121 DKEIMREIQAIMRQIASSITYLPCLDEPCVFDVLAYTDKDLAVPFTWIESDPKLIANPQM 180
           DKEIMREIQAIMRQIAS ITYLPCLDEPCVFDVLAYTD D+  P TW+ESD KLI NPQM
Sbjct: 121 DKEIMREIQAIMRQIASCITYLPCLDEPCVFDVLAYTDTDVDAPGTWVESDAKLIDNPQM 180

Query: 181 VKLHSFDTKIHKVDTLVSYKNDDCDDE 208
           VKLHSFDTKIHKVDTLVSYK D+ D+E
Sbjct: 181 VKLHSFDTKIHKVDTLVSYKKDEWDEE 207

BLAST of CmaCh04G018130 vs. Swiss-Prot
Match: MD2L1_DICDI (Mitotic spindle assembly checkpoint protein MAD2A OS=Dictyostelium discoideum GN=mad2l1-1 PE=3 SV=1)

HSP 1 Score: 223.4 bits (568), Expect = 2.3e-57
Identity = 112/200 (56.00%), Postives = 143/200 (71.50%), Query Frame = 1

Query: 1   MASKTASKDIITLRGSAAIVSEFFGYAANSILYNRGLYPEESFARVKKYGLPMLLTQDEG 60
           M +  ASK  I+L+GS  IV+EFF Y+ N+IL+ RGLYP ESF RV KYGLP+L+T D+ 
Sbjct: 1   MQAAVASKTNISLKGSTEIVTEFFSYSINTILFQRGLYPPESFTRVAKYGLPILVTNDQS 60

Query: 61  VKTFISNLTAQLSEWLEAGKLQRVVLVIMSKANNEVLERWNFRIETDGVVVEQGVSREKS 120
           +K ++ N+  QLSEWL +G +Q++VLVI      EVLERW F + TD +  E    R+K 
Sbjct: 61  LKDYLDNVLKQLSEWLLSGDVQKLVLVITDIVTKEVLERWVFDVTTD-IPKEGEAPRQKP 120

Query: 121 DKEIMREIQAIMRQIASSITYLPCLDEPCVFDVLAYTDKDLAVPFTWIESDPKLIANPQM 180
           +KEIM EIQAI+RQI +S+T+LP L   C FD+L YT KDLAVP  W ESDPK I N Q 
Sbjct: 121 EKEIMNEIQAIIRQITASVTFLPLLPNACTFDLLVYTSKDLAVPQKWEESDPKFITNSQQ 180

Query: 181 VKLHSFDTKIHKVDTLVSYK 201
           VKL SF T IHKV+++V+YK
Sbjct: 181 VKLRSFTTTIHKVESMVAYK 199

BLAST of CmaCh04G018130 vs. Swiss-Prot
Match: MD2L1_MOUSE (Mitotic spindle assembly checkpoint protein MAD2A OS=Mus musculus GN=Mad2l1 PE=1 SV=2)

HSP 1 Score: 208.0 bits (528), Expect = 1.0e-52
Identity = 103/200 (51.50%), Postives = 140/200 (70.00%), Query Frame = 1

Query: 1   MASKTASKDIITLRGSAAIVSEFFGYAANSILYNRGLYPEESFARVKKYGLPMLLTQDEG 60
           MA + A +  ITLRGSA IV+EFF +  NSILY RG+YP E+F RV+KYGL +L T D  
Sbjct: 1   MAQQLAREQGITLRGSAEIVAEFFSFGINSILYQRGIYPSETFTRVQKYGLTLLTTTDPE 60

Query: 61  VKTFISNLTAQLSEWLEAGKLQRVVLVIMSKANNEVLERWNFRIETDGVVVEQGVSREKS 120
           +  +++N+  QL EWL    +Q++V+VI +  + EVLERW F IE D    E+GV REKS
Sbjct: 61  LIKYLNNVVEQLKEWLYKCSVQKLVVVISNIESGEVLERWQFDIECDKTAKEEGVRREKS 120

Query: 121 DKEIMREIQAIMRQIASSITYLPCLDEPCVFDVLAYTDKDLAVPFTWIESDPKLIANPQM 180
            K I  EI++++RQI +++T+LP L+  C FD+L YTDKDL VP  W ES P+ I N + 
Sbjct: 121 QKAIQDEIRSVIRQITATVTFLPLLEVSCSFDLLIYTDKDLVVPEKWEESGPQFITNCEE 180

Query: 181 VKLHSFDTKIHKVDTLVSYK 201
           V+L SF T IHKV+++V+YK
Sbjct: 181 VRLRSFTTTIHKVNSMVAYK 200

BLAST of CmaCh04G018130 vs. Swiss-Prot
Match: MD2L1_HUMAN (Mitotic spindle assembly checkpoint protein MAD2A OS=Homo sapiens GN=MAD2L1 PE=1 SV=1)

HSP 1 Score: 199.9 bits (507), Expect = 2.7e-50
Identity = 98/200 (49.00%), Postives = 138/200 (69.00%), Query Frame = 1

Query: 1   MASKTASKDIITLRGSAAIVSEFFGYAANSILYNRGLYPEESFARVKKYGLPMLLTQDEG 60
           MA + + +  ITLRGSA IV+EFF +  NSILY RG+YP E+F RV+KYGL +L+T D  
Sbjct: 1   MALQLSREQGITLRGSAEIVAEFFSFGINSILYQRGIYPSETFTRVQKYGLTLLVTTDLE 60

Query: 61  VKTFISNLTAQLSEWLEAGKLQRVVLVIMSKANNEVLERWNFRIETDGVVVEQGVSREKS 120
           +  +++N+  QL +WL    +Q++V+VI +  + EVLERW F IE D    +    REKS
Sbjct: 61  LIKYLNNVVEQLKDWLYKCSVQKLVVVISNIESGEVLERWQFDIECDKTAKDDSAPREKS 120

Query: 121 DKEIMREIQAIMRQIASSITYLPCLDEPCVFDVLAYTDKDLAVPFTWIESDPKLIANPQM 180
            K I  EI++++RQI +++T+LP L+  C FD+L YTDKDL VP  W ES P+ I N + 
Sbjct: 121 QKAIQDEIRSVIRQITATVTFLPLLEVSCSFDLLIYTDKDLVVPEKWEESGPQFITNSEE 180

Query: 181 VKLHSFDTKIHKVDTLVSYK 201
           V+L SF T IHKV+++V+YK
Sbjct: 181 VRLRSFTTTIHKVNSMVAYK 200

BLAST of CmaCh04G018130 vs. TrEMBL
Match: A0A0A0KPY5_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G590170 PE=4 SV=1)

HSP 1 Score: 397.1 bits (1019), Expect = 1.3e-107
Identity = 201/207 (97.10%), Postives = 204/207 (98.55%), Query Frame = 1

Query: 1   MASKTASKDIITLRGSAAIVSEFFGYAANSILYNRGLYPEESFARVKKYGLPMLLTQDEG 60
           MASKTA+KDIITLRGSAAIVSEFFGYAANSILYNRGLYPEESF RVKKYGLPMLLTQDEG
Sbjct: 1   MASKTATKDIITLRGSAAIVSEFFGYAANSILYNRGLYPEESFVRVKKYGLPMLLTQDEG 60

Query: 61  VKTFISNLTAQLSEWLEAGKLQRVVLVIMSKANNEVLERWNFRIETDGVVVEQGVSREKS 120
           VKTFISNLTAQLSEWLEAGKLQRVVLVIMSK+NNEVLERWNFRIETDG VVEQGVSREKS
Sbjct: 61  VKTFISNLTAQLSEWLEAGKLQRVVLVIMSKSNNEVLERWNFRIETDGEVVEQGVSREKS 120

Query: 121 DKEIMREIQAIMRQIASSITYLPCLDEPCVFDVLAYTDKDLAVPFTWIESDPKLIANPQM 180
           DKEIMREIQAIMRQIASSITYLPCLDEPCVFDVLAYTDKDLAVPFTW+ESDPKLIANPQM
Sbjct: 121 DKEIMREIQAIMRQIASSITYLPCLDEPCVFDVLAYTDKDLAVPFTWMESDPKLIANPQM 180

Query: 181 VKLHSFDTKIHKVDTLVSYKNDDCDDE 208
           VKLHSFDTKIHKVDTLVSYKNDD DDE
Sbjct: 181 VKLHSFDTKIHKVDTLVSYKNDDDDDE 207

BLAST of CmaCh04G018130 vs. TrEMBL
Match: A0A061E776_THECC (DNA-binding HORMA family protein OS=Theobroma cacao GN=TCM_010752 PE=4 SV=1)

HSP 1 Score: 380.2 bits (975), Expect = 1.6e-102
Identity = 190/207 (91.79%), Postives = 201/207 (97.10%), Query Frame = 1

Query: 1   MASKTASKDIITLRGSAAIVSEFFGYAANSILYNRGLYPEESFARVKKYGLPMLLTQDEG 60
           MAS+TA+KDIITLRGSAAIVSEFFGYAANSILYNRG+YPEESF +VKKYGLPMLLT+DEG
Sbjct: 1   MASRTAAKDIITLRGSAAIVSEFFGYAANSILYNRGVYPEESFVKVKKYGLPMLLTEDEG 60

Query: 61  VKTFISNLTAQLSEWLEAGKLQRVVLVIMSKANNEVLERWNFRIETDGVVVEQGVSREKS 120
           VK+FI+NLTAQLSEWLEAGKLQRVVLVIMSKA NEVLERWNF IETD  VVE+GVSREKS
Sbjct: 61  VKSFITNLTAQLSEWLEAGKLQRVVLVIMSKATNEVLERWNFSIETDSEVVEKGVSREKS 120

Query: 121 DKEIMREIQAIMRQIASSITYLPCLDEPCVFDVLAYTDKDLAVPFTWIESDPKLIANPQM 180
           DKEIMREIQAIMRQIASSITYLPCLDEPCVFDVLAYTDKD+AVPFTWIESDPKLIANPQM
Sbjct: 121 DKEIMREIQAIMRQIASSITYLPCLDEPCVFDVLAYTDKDVAVPFTWIESDPKLIANPQM 180

Query: 181 VKLHSFDTKIHKVDTLVSYKNDDCDDE 208
           VKLHSFDTKIHKVDTLVSYKNDD D++
Sbjct: 181 VKLHSFDTKIHKVDTLVSYKNDDWDEQ 207

BLAST of CmaCh04G018130 vs. TrEMBL
Match: A0A0D2PKM1_GOSRA (Uncharacterized protein OS=Gossypium raimondii GN=B456_005G172500 PE=4 SV=1)

HSP 1 Score: 378.3 bits (970), Expect = 6.2e-102
Identity = 190/207 (91.79%), Postives = 201/207 (97.10%), Query Frame = 1

Query: 1   MASKTASKDIITLRGSAAIVSEFFGYAANSILYNRGLYPEESFARVKKYGLPMLLTQDEG 60
           MAS+T +KDIITLRGSAAIVSEFFGYAANSILYNRG+YPEESFA+VKKYGLPMLLTQDEG
Sbjct: 1   MASRTVAKDIITLRGSAAIVSEFFGYAANSILYNRGVYPEESFAKVKKYGLPMLLTQDEG 60

Query: 61  VKTFISNLTAQLSEWLEAGKLQRVVLVIMSKANNEVLERWNFRIETDGVVVEQGVSREKS 120
           VK+FISNLTAQLSEWLEAGKLQRVVLVIMSKA NEVLERWNF IETD  VVE+GVSREKS
Sbjct: 61  VKSFISNLTAQLSEWLEAGKLQRVVLVIMSKATNEVLERWNFSIETDCEVVEKGVSREKS 120

Query: 121 DKEIMREIQAIMRQIASSITYLPCLDEPCVFDVLAYTDKDLAVPFTWIESDPKLIANPQM 180
           DKEIMREIQAIMRQIASSITYLPCLDEPCVFDVLAYTDKD+AVPFTWIESDPKLIANPQM
Sbjct: 121 DKEIMREIQAIMRQIASSITYLPCLDEPCVFDVLAYTDKDVAVPFTWIESDPKLIANPQM 180

Query: 181 VKLHSFDTKIHKVDTLVSYKNDDCDDE 208
           VKLHSFDTKIHKVDTLVSYKND+ +++
Sbjct: 181 VKLHSFDTKIHKVDTLVSYKNDEWEEQ 207

BLAST of CmaCh04G018130 vs. TrEMBL
Match: D7TAM3_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_01s0010g02430 PE=4 SV=1)

HSP 1 Score: 377.1 bits (967), Expect = 1.4e-101
Identity = 188/207 (90.82%), Postives = 199/207 (96.14%), Query Frame = 1

Query: 1   MASKTASKDIITLRGSAAIVSEFFGYAANSILYNRGLYPEESFARVKKYGLPMLLTQDEG 60
           MAS+TA+KDIITLRGSAAIVSEFFGYAANSILYNRG+YPEESF +VKKYGLPMLLTQDEG
Sbjct: 1   MASRTATKDIITLRGSAAIVSEFFGYAANSILYNRGVYPEESFGKVKKYGLPMLLTQDEG 60

Query: 61  VKTFISNLTAQLSEWLEAGKLQRVVLVIMSKANNEVLERWNFRIETDGVVVEQGVSREKS 120
           VK+FI+NLTAQLSEWLE GKLQRVVLVIMSKA NEVLERWNF IETDG VVE GVSREKS
Sbjct: 61  VKSFIANLTAQLSEWLETGKLQRVVLVIMSKATNEVLERWNFSIETDGEVVENGVSREKS 120

Query: 121 DKEIMREIQAIMRQIASSITYLPCLDEPCVFDVLAYTDKDLAVPFTWIESDPKLIANPQM 180
           DKEIMREIQAIMRQIASSITYLPC+DEPCVFDVLAYTD D+AVPFTWIESDPKLIANPQM
Sbjct: 121 DKEIMREIQAIMRQIASSITYLPCIDEPCVFDVLAYTDTDVAVPFTWIESDPKLIANPQM 180

Query: 181 VKLHSFDTKIHKVDTLVSYKNDDCDDE 208
           VKLHSFDTKIHKVDTLVSYKND+ D++
Sbjct: 181 VKLHSFDTKIHKVDTLVSYKNDEWDED 207

BLAST of CmaCh04G018130 vs. TrEMBL
Match: B9HLA6_POPTR (Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0008s17970g PE=4 SV=2)

HSP 1 Score: 374.8 bits (961), Expect = 6.9e-101
Identity = 188/207 (90.82%), Postives = 198/207 (95.65%), Query Frame = 1

Query: 1   MASKTASKDIITLRGSAAIVSEFFGYAANSILYNRGLYPEESFARVKKYGLPMLLTQDEG 60
           MAS+T +KDIITLRGSAAIVSEFFGYAANSILYNRG+YPEESF +VKKYGLPMLLTQDEG
Sbjct: 61  MASRTVAKDIITLRGSAAIVSEFFGYAANSILYNRGVYPEESFVKVKKYGLPMLLTQDEG 120

Query: 61  VKTFISNLTAQLSEWLEAGKLQRVVLVIMSKANNEVLERWNFRIETDGVVVEQGVSREKS 120
           V++FISNL AQLSEWLEAGKLQRVVLVIMSKA NEVLERWNF IETD  VVEQGVSREKS
Sbjct: 121 VRSFISNLNAQLSEWLEAGKLQRVVLVIMSKATNEVLERWNFSIETDSEVVEQGVSREKS 180

Query: 121 DKEIMREIQAIMRQIASSITYLPCLDEPCVFDVLAYTDKDLAVPFTWIESDPKLIANPQM 180
           DKEIMREIQAIMRQIASSITYLPCLDE CVFDVLAYTDKD+AVPFTWIESDPKLIANPQM
Sbjct: 181 DKEIMREIQAIMRQIASSITYLPCLDESCVFDVLAYTDKDVAVPFTWIESDPKLIANPQM 240

Query: 181 VKLHSFDTKIHKVDTLVSYKNDDCDDE 208
           VKLHSFDTKIHKVDTLVSYKND+ D++
Sbjct: 241 VKLHSFDTKIHKVDTLVSYKNDEWDEQ 267

BLAST of CmaCh04G018130 vs. TAIR10
Match: AT3G25980.1 (AT3G25980.1 DNA-binding HORMA family protein)

HSP 1 Score: 353.2 bits (905), Expect = 1.1e-97
Identity = 178/208 (85.58%), Postives = 193/208 (92.79%), Query Frame = 1

Query: 1   MASKTAS-KDIITLRGSAAIVSEFFGYAANSILYNRGLYPEESFARVKKYGLPMLLTQDE 60
           MASKTA+ KDIITL GSAAIVSEFF YAANSILYNR +YPEESF +VKKYGLPMLL +DE
Sbjct: 1   MASKTAAAKDIITLHGSAAIVSEFFCYAANSILYNRAVYPEESFVKVKKYGLPMLLIEDE 60

Query: 61  GVKTFISNLTAQLSEWLEAGKLQRVVLVIMSKANNEVLERWNFRIETDGVVVEQGVSREK 120
            VK+F+SNLT+Q+SEWLEAGKLQRVVLVIMSKA  EVLERWNFRIETD  VV++GVSREK
Sbjct: 61  SVKSFMSNLTSQISEWLEAGKLQRVVLVIMSKATGEVLERWNFRIETDNEVVDKGVSREK 120

Query: 121 SDKEIMREIQAIMRQIASSITYLPCLDEPCVFDVLAYTDKDLAVPFTWIESDPKLIANPQ 180
           SDKEIMREIQAIMRQ+ASS+TYLPCLDE CVFDVLAYTD D+AVPFTWIESDPKLIANPQ
Sbjct: 121 SDKEIMREIQAIMRQVASSVTYLPCLDETCVFDVLAYTDTDVAVPFTWIESDPKLIANPQ 180

Query: 181 MVKLHSFDTKIHKVDTLVSYKNDDCDDE 208
           MVKLH FDTKIHKVDTLVSYKND+ D+E
Sbjct: 181 MVKLHGFDTKIHKVDTLVSYKNDEWDEE 208

BLAST of CmaCh04G018130 vs. NCBI nr
Match: gi|449435035|ref|XP_004135301.1| (PREDICTED: mitotic spindle checkpoint protein MAD2 [Cucumis sativus])

HSP 1 Score: 397.1 bits (1019), Expect = 1.8e-107
Identity = 201/207 (97.10%), Postives = 204/207 (98.55%), Query Frame = 1

Query: 1   MASKTASKDIITLRGSAAIVSEFFGYAANSILYNRGLYPEESFARVKKYGLPMLLTQDEG 60
           MASKTA+KDIITLRGSAAIVSEFFGYAANSILYNRGLYPEESF RVKKYGLPMLLTQDEG
Sbjct: 1   MASKTATKDIITLRGSAAIVSEFFGYAANSILYNRGLYPEESFVRVKKYGLPMLLTQDEG 60

Query: 61  VKTFISNLTAQLSEWLEAGKLQRVVLVIMSKANNEVLERWNFRIETDGVVVEQGVSREKS 120
           VKTFISNLTAQLSEWLEAGKLQRVVLVIMSK+NNEVLERWNFRIETDG VVEQGVSREKS
Sbjct: 61  VKTFISNLTAQLSEWLEAGKLQRVVLVIMSKSNNEVLERWNFRIETDGEVVEQGVSREKS 120

Query: 121 DKEIMREIQAIMRQIASSITYLPCLDEPCVFDVLAYTDKDLAVPFTWIESDPKLIANPQM 180
           DKEIMREIQAIMRQIASSITYLPCLDEPCVFDVLAYTDKDLAVPFTW+ESDPKLIANPQM
Sbjct: 121 DKEIMREIQAIMRQIASSITYLPCLDEPCVFDVLAYTDKDLAVPFTWMESDPKLIANPQM 180

Query: 181 VKLHSFDTKIHKVDTLVSYKNDDCDDE 208
           VKLHSFDTKIHKVDTLVSYKNDD DDE
Sbjct: 181 VKLHSFDTKIHKVDTLVSYKNDDDDDE 207

BLAST of CmaCh04G018130 vs. NCBI nr
Match: gi|659090572|ref|XP_008446088.1| (PREDICTED: mitotic spindle checkpoint protein MAD2 [Cucumis melo])

HSP 1 Score: 391.7 bits (1005), Expect = 7.8e-106
Identity = 198/207 (95.65%), Postives = 203/207 (98.07%), Query Frame = 1

Query: 1   MASKTASKDIITLRGSAAIVSEFFGYAANSILYNRGLYPEESFARVKKYGLPMLLTQDEG 60
           MASKTA+KDIITLRGSAAIVSEFFGYAANSILYNRGLYPEESF RVKKYGLPMLLTQDEG
Sbjct: 1   MASKTATKDIITLRGSAAIVSEFFGYAANSILYNRGLYPEESFVRVKKYGLPMLLTQDEG 60

Query: 61  VKTFISNLTAQLSEWLEAGKLQRVVLVIMSKANNEVLERWNFRIETDGVVVEQGVSREKS 120
           VK+FISNLTAQLSEWLEAGKLQRVVLVIMSK+NNEVLERWNFRIETDG VVEQGVSREKS
Sbjct: 61  VKSFISNLTAQLSEWLEAGKLQRVVLVIMSKSNNEVLERWNFRIETDGEVVEQGVSREKS 120

Query: 121 DKEIMREIQAIMRQIASSITYLPCLDEPCVFDVLAYTDKDLAVPFTWIESDPKLIANPQM 180
           DKEIMREIQAIMRQIASSITYLPCLDEPCVFDVLAYTDKDLAVPFTW+ESDPKLIANPQM
Sbjct: 121 DKEIMREIQAIMRQIASSITYLPCLDEPCVFDVLAYTDKDLAVPFTWMESDPKLIANPQM 180

Query: 181 VKLHSFDTKIHKVDTLVSYKNDDCDDE 208
           VKLHSFDTKIHKVDTLVSYKNDD  D+
Sbjct: 181 VKLHSFDTKIHKVDTLVSYKNDDDGDD 207

BLAST of CmaCh04G018130 vs. NCBI nr
Match: gi|590695773|ref|XP_007044984.1| (DNA-binding HORMA family protein [Theobroma cacao])

HSP 1 Score: 380.2 bits (975), Expect = 2.3e-102
Identity = 190/207 (91.79%), Postives = 201/207 (97.10%), Query Frame = 1

Query: 1   MASKTASKDIITLRGSAAIVSEFFGYAANSILYNRGLYPEESFARVKKYGLPMLLTQDEG 60
           MAS+TA+KDIITLRGSAAIVSEFFGYAANSILYNRG+YPEESF +VKKYGLPMLLT+DEG
Sbjct: 1   MASRTAAKDIITLRGSAAIVSEFFGYAANSILYNRGVYPEESFVKVKKYGLPMLLTEDEG 60

Query: 61  VKTFISNLTAQLSEWLEAGKLQRVVLVIMSKANNEVLERWNFRIETDGVVVEQGVSREKS 120
           VK+FI+NLTAQLSEWLEAGKLQRVVLVIMSKA NEVLERWNF IETD  VVE+GVSREKS
Sbjct: 61  VKSFITNLTAQLSEWLEAGKLQRVVLVIMSKATNEVLERWNFSIETDSEVVEKGVSREKS 120

Query: 121 DKEIMREIQAIMRQIASSITYLPCLDEPCVFDVLAYTDKDLAVPFTWIESDPKLIANPQM 180
           DKEIMREIQAIMRQIASSITYLPCLDEPCVFDVLAYTDKD+AVPFTWIESDPKLIANPQM
Sbjct: 121 DKEIMREIQAIMRQIASSITYLPCLDEPCVFDVLAYTDKDVAVPFTWIESDPKLIANPQM 180

Query: 181 VKLHSFDTKIHKVDTLVSYKNDDCDDE 208
           VKLHSFDTKIHKVDTLVSYKNDD D++
Sbjct: 181 VKLHSFDTKIHKVDTLVSYKNDDWDEQ 207

BLAST of CmaCh04G018130 vs. NCBI nr
Match: gi|702277798|ref|XP_010044758.1| (PREDICTED: mitotic spindle checkpoint protein MAD2 [Eucalyptus grandis])

HSP 1 Score: 379.0 bits (972), Expect = 5.2e-102
Identity = 190/207 (91.79%), Postives = 200/207 (96.62%), Query Frame = 1

Query: 1   MASKTASKDIITLRGSAAIVSEFFGYAANSILYNRGLYPEESFARVKKYGLPMLLTQDEG 60
           MASKT +KDIITLRGSAAIVSEFFGYAANSILYNRG+YPEESFA+VKKYGLPMLLTQDEG
Sbjct: 1   MASKTVAKDIITLRGSAAIVSEFFGYAANSILYNRGVYPEESFAKVKKYGLPMLLTQDEG 60

Query: 61  VKTFISNLTAQLSEWLEAGKLQRVVLVIMSKANNEVLERWNFRIETDGVVVEQGVSREKS 120
           VK+FI+NL AQLSEWLEAGKLQRVVLVIMSKA  EVLERWNF IETDG VVE+GVSREKS
Sbjct: 61  VKSFIANLNAQLSEWLEAGKLQRVVLVIMSKATGEVLERWNFSIETDGEVVEKGVSREKS 120

Query: 121 DKEIMREIQAIMRQIASSITYLPCLDEPCVFDVLAYTDKDLAVPFTWIESDPKLIANPQM 180
           DKEIMREIQAIMRQIASSITYLPCLDEPCVFDVLAYTDKD+AVPFTWIESDPKLIANPQM
Sbjct: 121 DKEIMREIQAIMRQIASSITYLPCLDEPCVFDVLAYTDKDVAVPFTWIESDPKLIANPQM 180

Query: 181 VKLHSFDTKIHKVDTLVSYKNDDCDDE 208
           VKLHSFDTKIHKVDTLVSYKND+ D++
Sbjct: 181 VKLHSFDTKIHKVDTLVSYKNDEWDEQ 207

BLAST of CmaCh04G018130 vs. NCBI nr
Match: gi|823158792|ref|XP_012479233.1| (PREDICTED: mitotic spindle checkpoint protein MAD2 [Gossypium raimondii])

HSP 1 Score: 378.3 bits (970), Expect = 8.9e-102
Identity = 190/207 (91.79%), Postives = 201/207 (97.10%), Query Frame = 1

Query: 1   MASKTASKDIITLRGSAAIVSEFFGYAANSILYNRGLYPEESFARVKKYGLPMLLTQDEG 60
           MAS+T +KDIITLRGSAAIVSEFFGYAANSILYNRG+YPEESFA+VKKYGLPMLLTQDEG
Sbjct: 1   MASRTVAKDIITLRGSAAIVSEFFGYAANSILYNRGVYPEESFAKVKKYGLPMLLTQDEG 60

Query: 61  VKTFISNLTAQLSEWLEAGKLQRVVLVIMSKANNEVLERWNFRIETDGVVVEQGVSREKS 120
           VK+FISNLTAQLSEWLEAGKLQRVVLVIMSKA NEVLERWNF IETD  VVE+GVSREKS
Sbjct: 61  VKSFISNLTAQLSEWLEAGKLQRVVLVIMSKATNEVLERWNFSIETDCEVVEKGVSREKS 120

Query: 121 DKEIMREIQAIMRQIASSITYLPCLDEPCVFDVLAYTDKDLAVPFTWIESDPKLIANPQM 180
           DKEIMREIQAIMRQIASSITYLPCLDEPCVFDVLAYTDKD+AVPFTWIESDPKLIANPQM
Sbjct: 121 DKEIMREIQAIMRQIASSITYLPCLDEPCVFDVLAYTDKDVAVPFTWIESDPKLIANPQM 180

Query: 181 VKLHSFDTKIHKVDTLVSYKNDDCDDE 208
           VKLHSFDTKIHKVDTLVSYKND+ +++
Sbjct: 181 VKLHSFDTKIHKVDTLVSYKNDEWEEQ 207

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
MAD2_ARATH1.9e-9685.58Mitotic spindle checkpoint protein MAD2 OS=Arabidopsis thaliana GN=MAD2 PE=1 SV=... [more]
MAD2_MAIZE2.6e-9383.09Mitotic spindle checkpoint protein MAD2 OS=Zea mays GN=MAD2 PE=2 SV=1[more]
MD2L1_DICDI2.3e-5756.00Mitotic spindle assembly checkpoint protein MAD2A OS=Dictyostelium discoideum GN... [more]
MD2L1_MOUSE1.0e-5251.50Mitotic spindle assembly checkpoint protein MAD2A OS=Mus musculus GN=Mad2l1 PE=1... [more]
MD2L1_HUMAN2.7e-5049.00Mitotic spindle assembly checkpoint protein MAD2A OS=Homo sapiens GN=MAD2L1 PE=1... [more]
Match NameE-valueIdentityDescription
A0A0A0KPY5_CUCSA1.3e-10797.10Uncharacterized protein OS=Cucumis sativus GN=Csa_5G590170 PE=4 SV=1[more]
A0A061E776_THECC1.6e-10291.79DNA-binding HORMA family protein OS=Theobroma cacao GN=TCM_010752 PE=4 SV=1[more]
A0A0D2PKM1_GOSRA6.2e-10291.79Uncharacterized protein OS=Gossypium raimondii GN=B456_005G172500 PE=4 SV=1[more]
D7TAM3_VITVI1.4e-10190.82Putative uncharacterized protein OS=Vitis vinifera GN=VIT_01s0010g02430 PE=4 SV=... [more]
B9HLA6_POPTR6.9e-10190.82Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0008s17970g PE=4 SV=2[more]
Match NameE-valueIdentityDescription
AT3G25980.11.1e-9785.58 DNA-binding HORMA family protein[more]
Match NameE-valueIdentityDescription
gi|449435035|ref|XP_004135301.1|1.8e-10797.10PREDICTED: mitotic spindle checkpoint protein MAD2 [Cucumis sativus][more]
gi|659090572|ref|XP_008446088.1|7.8e-10695.65PREDICTED: mitotic spindle checkpoint protein MAD2 [Cucumis melo][more]
gi|590695773|ref|XP_007044984.1|2.3e-10291.79DNA-binding HORMA family protein [Theobroma cacao][more]
gi|702277798|ref|XP_010044758.1|5.2e-10291.79PREDICTED: mitotic spindle checkpoint protein MAD2 [Eucalyptus grandis][more]
gi|823158792|ref|XP_012479233.1|8.9e-10291.79PREDICTED: mitotic spindle checkpoint protein MAD2 [Gossypium raimondii][more]
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR003511HORMA_dom
IPR027097Mad2
Vocabulary: Biological Process
TermDefinition
GO:0007094mitotic spindle assembly checkpoint
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008283 cell proliferation
biological_process GO:0042023 DNA endoreduplication
biological_process GO:0016572 histone phosphorylation
biological_process GO:0007094 mitotic spindle assembly checkpoint
biological_process GO:0043248 proteasome assembly
biological_process GO:0051510 regulation of unidimensional cell growth
biological_process GO:0051788 response to misfolded protein
cellular_component GO:0010369 chromocenter
cellular_component GO:0005737 cytoplasm
cellular_component GO:0000776 kinetochore
cellular_component GO:0005828 kinetochore microtubule
cellular_component GO:0005635 nuclear envelope
cellular_component GO:0005575 cellular_component
molecular_function GO:0003677 DNA binding
molecular_function GO:0003674 molecular_function

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh04G018130.1CmaCh04G018130.1mRNA


Analysis Name: InterPro Annotations of Cucurbita maxima
Date Performed: 2017-05-20
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR003511HORMA domainGENE3DG3DSA:3.30.900.10coord: 1..203
score: 2.0
IPR003511HORMA domainPFAMPF02301HORMAcoord: 16..191
score: 5.4
IPR003511HORMA domainPROFILEPS50815HORMAcoord: 14..197
score: 45
IPR003511HORMA domainunknownSSF56019The spindle assembly checkpoint protein mad2coord: 10..204
score: 1.31
IPR027097Mitotic spindle checkpoint protein Mad2PANTHERPTHR11842:SF11MITOTIC SPINDLE ASSEMBLY CHECKPOINT PROTEIN MAD2Acoord: 1..207
score: 8.6E
NoneNo IPR availablePANTHERPTHR11842MITOTIC SPINDLE ASSEMBLY CHECKPOINT PROTEIN MAD2coord: 1..207
score: 8.6E

The following gene(s) are paralogous to this gene:
GeneParalogueOrganismBlock
CmaCh04G018130CmaCh18G007210Cucurbita maxima (Rimu)cmacmaB404